AlfalfaGEDB | Msa1418150

M. sativa cultivar zhongmu-4 / Msa1418150

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418150	A0A7J8WH47	91.765	85	7	0	1	85	124	208	1.16e-52	172

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418150	MtrunA17_Chr6g0480541	92.941	85	6	0	1	85	1125	1209	2.43e-52	174
Msa1418150	MtrunA17_Chr6g0480291	88.235	85	9	1	1	85	1	84	3.16e-50	152
Msa1418150	MtrunA17_Chr5g0437131	56.627	83	36	0	1	83	630	712	9.15e-29	107
Msa1418150	MtrunA17_Chr6g0480191	92.857	42	3	0	44	85	1	42	3.09e-24	85.9
Msa1418150	MtrunA17_Chr6g0480331	85.366	41	6	0	23	63	2	42	2.98e-21	78.6
Msa1418150	MtrunA17_Chr6g0480471	92.308	26	2	0	1	26	50	75	1.17e-11	55.1

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418150	AT1G73450.1	83.529	85	14	1	85	1068	1152	2.97e-48	163
Msa1418150	AT1G73460.2	83.529	85	14	1	85	1085	1169	3.47e-48	163
Msa1418150	AT1G73460.1	83.529	85	14	1	85	1085	1169	3.47e-48	163
Msa1418150	AT3G17750.1	82.353	85	15	1	85	1054	1138	9.83e-46	156
Msa1418150	AT4G03175.1	53.247	77	36	1	77	17	93	1.92e-26	93.6
Msa1418150	AT2G40120.1	51.948	77	37	1	77	488	564	4.94e-23	91.3

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Find 24 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATATGCTTTATGAG+AGG	0.623112	tig0024536:-78524	Msa1418150:CDS
AAAGTATGCTAGCAAAAGGA+CGG	0.592834	tig0024536:-78569	Msa1418150:exon
AAGATGATATCGGTTCGTAA+GGG	0.655236	tig0024536:+77823	None:intergenic
AAGATGTCTTGTTTGGAATC+AGG	0.354171	tig0024536:+77967	None:intergenic
AAGTATGCTAGCAAAAGGAC+GGG	0.589744	tig0024536:-78568	Msa1418150:exon
AGAATACTTCAAGATGATAT+CGG	0.467186	tig0024536:+77813	None:intergenic
ATACAGAAGGTCGCTTCTTC+GGG	0.333095	tig0024536:+77877	None:intergenic
CAACAAAGTCAATGAAGCCT+TGG	0.499867	tig0024536:+77919	None:intergenic
CAAGATGATATCGGTTCGTA+AGG	0.480516	tig0024536:+77822	None:intergenic
CGTAAGGGTATGATAACCAG+GGG	0.686121	tig0024536:+77838	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATATGAGCATGTTTGGATT+GGG	+	tig0024536:78315-78334	None:intergenic	30.0%
TAATATGAGCATGTTTGGAT+TGG	+	tig0024536:78316-78335	None:intergenic	30.0%
AATATGAGCATGTTTGGATT+GGG	+	tig0024536:78315-78334	None:intergenic	30.0%
TAATATGAGCATGTTTGGAT+TGG	+	tig0024536:78316-78335	None:intergenic	30.0%
AAAGTATGCTAGCAAAAGGA+CGG	-	tig0024536:77821-77840	Msa1418150:CDS	35.0%
TATGCTTTATGAGAGGAATC+AGG	-	tig0024536:77873-77892	Msa1418150:CDS	35.0%
TTGCAATCCTAGAGAGTTTA+CGG	-	tig0024536:78189-78208	Msa1418150:intron	35.0%
GTAATTTCCGTAAACTCTCT+AGG	+	tig0024536:78199-78218	None:intergenic	35.0%
TTCCAAACAAGACATCTTTG+AGG	-	tig0024536:78428-78447	Msa1418150:intron	35.0%
AAAGTATGCTAGCAAAAGGA+CGG	-	tig0024536:77821-77840	Msa1418150:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024536	gene	77821	78591	77821	ID=Msa1418150;Name=Msa1418150
tig0024536	mRNA	77821	78591	77821	ID=Msa1418150-mRNA-1;Parent=Msa1418150;Name=Msa1418150-mRNA-1;_AED=0.27;_eAED=0.27;_QI=5\|1\|0.5\|1\|0\|0.5\|2\|0\|85
tig0024536	exon	77821	78009	77821	ID=Msa1418150-mRNA-1:exon:210;Parent=Msa1418150-mRNA-1
tig0024536	exon	78518	78591	78518	ID=Msa1418150-mRNA-1:exon:209;Parent=Msa1418150-mRNA-1
tig0024536	five_prime_UTR	78587	78591	78587	ID=Msa1418150-mRNA-1:five_prime_utr;Parent=Msa1418150-mRNA-1
tig0024536	CDS	78518	78586	78518	ID=Msa1418150-mRNA-1:cds;Parent=Msa1418150-mRNA-1
tig0024536	CDS	77821	78009	77821	ID=Msa1418150-mRNA-1:cds;Parent=Msa1418150-mRNA-1

Gene Sequence	>Msa1418150 ATGCTAGCAAAAGGACGGGATACTTATAAGTACTTCACAAAAAATCATATGCTTTATGAGAGGAATCAGGAAAGCAACAAATTTGAATACCTGATTCCAAACAAGACATCTTTGAGGCATAGATTACCGATGGGGGACCAAGGCTTCATTGACTTTGTTGCTCATTTGCTCGAGGTTAACCCGAAGAAGCGACCTTCTGTATCTCAAGCTCTAAAGCACCCCTGGTTATCATACCCTTACGAACCGATATCATCTTGA
Protein sequence	>Msa1418150 MLAKGRDTYKYFTKNHMLYERNQESNKFEYLIPNKTSLRHRLPMGDQGFIDFVAHLLEVNPKKRPSVSQALKHPWLSYPYEPISS*