Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418150 | A0A7J8WH47 | 91.765 | 85 | 7 | 0 | 1 | 85 | 124 | 208 | 1.16e-52 | 172 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418150 | MtrunA17_Chr6g0480541 | 92.941 | 85 | 6 | 0 | 1 | 85 | 1125 | 1209 | 2.43e-52 | 174 |
| Msa1418150 | MtrunA17_Chr6g0480291 | 88.235 | 85 | 9 | 1 | 1 | 85 | 1 | 84 | 3.16e-50 | 152 |
| Msa1418150 | MtrunA17_Chr5g0437131 | 56.627 | 83 | 36 | 0 | 1 | 83 | 630 | 712 | 9.15e-29 | 107 |
| Msa1418150 | MtrunA17_Chr6g0480191 | 92.857 | 42 | 3 | 0 | 44 | 85 | 1 | 42 | 3.09e-24 | 85.9 |
| Msa1418150 | MtrunA17_Chr6g0480331 | 85.366 | 41 | 6 | 0 | 23 | 63 | 2 | 42 | 2.98e-21 | 78.6 |
| Msa1418150 | MtrunA17_Chr6g0480471 | 92.308 | 26 | 2 | 0 | 1 | 26 | 50 | 75 | 1.17e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418150 | AT1G73450.1 | 83.529 | 85 | 14 | 0 | 1 | 85 | 1068 | 1152 | 2.97e-48 | 163 |
| Msa1418150 | AT1G73460.2 | 83.529 | 85 | 14 | 0 | 1 | 85 | 1085 | 1169 | 3.47e-48 | 163 |
| Msa1418150 | AT1G73460.1 | 83.529 | 85 | 14 | 0 | 1 | 85 | 1085 | 1169 | 3.47e-48 | 163 |
| Msa1418150 | AT3G17750.1 | 82.353 | 85 | 15 | 0 | 1 | 85 | 1054 | 1138 | 9.83e-46 | 156 |
| Msa1418150 | AT4G03175.1 | 53.247 | 77 | 36 | 0 | 1 | 77 | 17 | 93 | 1.92e-26 | 93.6 |
| Msa1418150 | AT2G40120.1 | 51.948 | 77 | 37 | 0 | 1 | 77 | 488 | 564 | 4.94e-23 | 91.3 |
Find 24 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACAGAAGGTCGCTTCTTC+GGG | 0.333095 | tig0024536:+77877 | None:intergenic |
| GATACAGAAGGTCGCTTCTT+CGG | 0.340198 | tig0024536:+77876 | None:intergenic |
| TGCCTCAAAGATGTCTTGTT+TGG | 0.351784 | tig0024536:+77960 | None:intergenic |
| AAGATGTCTTGTTTGGAATC+AGG | 0.354171 | tig0024536:+77967 | None:intergenic |
| AGAATACTTCAAGATGATAT+CGG | 0.467186 | tig0024536:+77813 | None:intergenic |
| CAAGATGATATCGGTTCGTA+AGG | 0.480516 | tig0024536:+77822 | None:intergenic |
| CTCAAGCTCTAAAGCACCCC+TGG | 0.493867 | tig0024536:-77854 | Msa1418150:CDS |
| TTCCAAACAAGACATCTTTG+AGG | 0.495846 | tig0024536:-77962 | Msa1418150:CDS |
| GGTCAAAGTATGCTAGCAAA+AGG | 0.496032 | tig0024536:-78573 | None:intergenic |
| CAACAAAGTCAATGAAGCCT+TGG | 0.499867 | tig0024536:+77919 | None:intergenic |
| TATGCTTTATGAGAGGAATC+AGG | 0.504464 | tig0024536:-78517 | Msa1418150:intron |
| TTCGTAAGGGTATGATAACC+AGG | 0.523874 | tig0024536:+77836 | None:intergenic |
| TTTGAGGCATAGATTACCGA+TGG | 0.557764 | tig0024536:-77946 | Msa1418150:CDS |
| CTTTGTTGCTCATTTGCTCG+AGG | 0.575791 | tig0024536:-77904 | Msa1418150:CDS |
| AAGTATGCTAGCAAAAGGAC+GGG | 0.589744 | tig0024536:-78568 | Msa1418150:exon |
| AAAGTATGCTAGCAAAAGGA+CGG | 0.592834 | tig0024536:-78569 | Msa1418150:exon |
| TTGAGGCATAGATTACCGAT+GGG | 0.601000 | tig0024536:-77945 | Msa1418150:CDS |
| AAAATCATATGCTTTATGAG+AGG | 0.623112 | tig0024536:-78524 | Msa1418150:CDS |
| TTTAGAGCTTGAGATACAGA+AGG | 0.647239 | tig0024536:+77864 | None:intergenic |
| AAGATGATATCGGTTCGTAA+GGG | 0.655236 | tig0024536:+77823 | None:intergenic |
| TCGTAAGGGTATGATAACCA+GGG | 0.667962 | tig0024536:+77837 | None:intergenic |
| CGTAAGGGTATGATAACCAG+GGG | 0.686121 | tig0024536:+77838 | None:intergenic |
| TGAGGCATAGATTACCGATG+GGG | 0.713357 | tig0024536:-77944 | Msa1418150:CDS |
| GAGGCATAGATTACCGATGG+GGG | 0.717861 | tig0024536:-77943 | Msa1418150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAGAAAATAACATATAT+AGG | + | tig0024536:78115-78134 | None:intergenic | 10.0% |
| !! | TTATAGAAAATAACATATAT+AGG | + | tig0024536:78115-78134 | None:intergenic | 10.0% |
| !!! | AGTCAATTTTAGAAATTTTA+GGG | + | tig0024536:78013-78032 | None:intergenic | 15.0% |
| !!! | TAGTCAATTTTAGAAATTTT+AGG | + | tig0024536:78014-78033 | None:intergenic | 15.0% |
| !! | ACAACTTATATGAAAATAAT+TGG | + | tig0024536:78156-78175 | None:intergenic | 15.0% |
| !!! | AGTCAATTTTAGAAATTTTA+GGG | + | tig0024536:78013-78032 | None:intergenic | 15.0% |
| !!! | TAGTCAATTTTAGAAATTTT+AGG | + | tig0024536:78014-78033 | None:intergenic | 15.0% |
| !! | ACAACTTATATGAAAATAAT+TGG | + | tig0024536:78156-78175 | None:intergenic | 15.0% |
| !! | TTAAATTGTTTATCCAAACA+TGG | + | tig0024536:78057-78076 | None:intergenic | 20.0% |
| !! | CATTGTATGTTTAATTATCA+TGG | - | tig0024536:78342-78361 | Msa1418150:intron | 20.0% |
| !! | TTAAATTGTTTATCCAAACA+TGG | + | tig0024536:78057-78076 | None:intergenic | 20.0% |
| !! | CATTGTATGTTTAATTATCA+TGG | - | tig0024536:78342-78361 | Msa1418150:intron | 20.0% |
| ! | AAAATCATATGCTTTATGAG+AGG | - | tig0024536:77866-77885 | Msa1418150:CDS | 25.0% |
| ! | ATGTTTAATATGAGCATGTT+TGG | + | tig0024536:78321-78340 | None:intergenic | 25.0% |
| ! | CATGGAAAAATTATTCATTG+AGG | - | tig0024536:78360-78379 | Msa1418150:intron | 25.0% |
| ! | AAAATCATATGCTTTATGAG+AGG | - | tig0024536:77866-77885 | Msa1418150:CDS | 25.0% |
| ! | ATGTTTAATATGAGCATGTT+TGG | + | tig0024536:78321-78340 | None:intergenic | 25.0% |
| ! | CATGGAAAAATTATTCATTG+AGG | - | tig0024536:78360-78379 | Msa1418150:intron | 25.0% |
| AATATGAGCATGTTTGGATT+GGG | + | tig0024536:78315-78334 | None:intergenic | 30.0% | |
| TAATATGAGCATGTTTGGAT+TGG | + | tig0024536:78316-78335 | None:intergenic | 30.0% | |
| AATATGAGCATGTTTGGATT+GGG | + | tig0024536:78315-78334 | None:intergenic | 30.0% | |
| TAATATGAGCATGTTTGGAT+TGG | + | tig0024536:78316-78335 | None:intergenic | 30.0% | |
| AAAGTATGCTAGCAAAAGGA+CGG | - | tig0024536:77821-77840 | Msa1418150:CDS | 35.0% | |
| TATGCTTTATGAGAGGAATC+AGG | - | tig0024536:77873-77892 | Msa1418150:CDS | 35.0% | |
| TTGCAATCCTAGAGAGTTTA+CGG | - | tig0024536:78189-78208 | Msa1418150:intron | 35.0% | |
| GTAATTTCCGTAAACTCTCT+AGG | + | tig0024536:78199-78218 | None:intergenic | 35.0% | |
| !! | AAGATGTCTTGTTTGGAATC+AGG | + | tig0024536:78426-78445 | None:intergenic | 35.0% |
| TTCCAAACAAGACATCTTTG+AGG | - | tig0024536:78428-78447 | Msa1418150:intron | 35.0% | |
| ! | TTTAGAGCTTGAGATACAGA+AGG | + | tig0024536:78529-78548 | None:intergenic | 35.0% |
| !! | AAGATGATATCGGTTCGTAA+GGG | + | tig0024536:78570-78589 | None:intergenic | 35.0% |
| AAAGTATGCTAGCAAAAGGA+CGG | - | tig0024536:77821-77840 | Msa1418150:CDS | 35.0% | |
| TATGCTTTATGAGAGGAATC+AGG | - | tig0024536:77873-77892 | Msa1418150:CDS | 35.0% | |
| TTGCAATCCTAGAGAGTTTA+CGG | - | tig0024536:78189-78208 | Msa1418150:intron | 35.0% | |
| GTAATTTCCGTAAACTCTCT+AGG | + | tig0024536:78199-78218 | None:intergenic | 35.0% | |
| !! | AAGATGTCTTGTTTGGAATC+AGG | + | tig0024536:78426-78445 | None:intergenic | 35.0% |
| TTCCAAACAAGACATCTTTG+AGG | - | tig0024536:78428-78447 | Msa1418150:intron | 35.0% | |
| ! | TTTAGAGCTTGAGATACAGA+AGG | + | tig0024536:78529-78548 | None:intergenic | 35.0% |
| !! | AAGATGATATCGGTTCGTAA+GGG | + | tig0024536:78570-78589 | None:intergenic | 35.0% |
| AAGTATGCTAGCAAAAGGAC+GGG | - | tig0024536:77822-77841 | Msa1418150:CDS | 40.0% | |
| TCGCTTGTATTTGCCATGTT+TGG | - | tig0024536:78041-78060 | Msa1418150:intron | 40.0% | |
| AGACTGCTTGGGAGAATTTA+TGG | + | tig0024536:78257-78276 | None:intergenic | 40.0% | |
| ! | GGCATTGTGTATTGATATGC+AGG | - | tig0024536:78381-78400 | Msa1418150:intron | 40.0% |
| ! | TGCCTCAAAGATGTCTTGTT+TGG | + | tig0024536:78433-78452 | None:intergenic | 40.0% |
| ! | TTTGAGGCATAGATTACCGA+TGG | - | tig0024536:78444-78463 | Msa1418150:intron | 40.0% |
| TTGAGGCATAGATTACCGAT+GGG | - | tig0024536:78445-78464 | Msa1418150:intron | 40.0% | |
| CAACAAAGTCAATGAAGCCT+TGG | + | tig0024536:78474-78493 | None:intergenic | 40.0% | |
| ! | TCGTAAGGGTATGATAACCA+GGG | + | tig0024536:78556-78575 | None:intergenic | 40.0% |
| ! | TTCGTAAGGGTATGATAACC+AGG | + | tig0024536:78557-78576 | None:intergenic | 40.0% |
| !! | CAAGATGATATCGGTTCGTA+AGG | + | tig0024536:78571-78590 | None:intergenic | 40.0% |
| AAGTATGCTAGCAAAAGGAC+GGG | - | tig0024536:77822-77841 | Msa1418150:CDS | 40.0% | |
| TCGCTTGTATTTGCCATGTT+TGG | - | tig0024536:78041-78060 | Msa1418150:intron | 40.0% | |
| AGACTGCTTGGGAGAATTTA+TGG | + | tig0024536:78257-78276 | None:intergenic | 40.0% | |
| ! | GGCATTGTGTATTGATATGC+AGG | - | tig0024536:78381-78400 | Msa1418150:intron | 40.0% |
| ! | TGCCTCAAAGATGTCTTGTT+TGG | + | tig0024536:78433-78452 | None:intergenic | 40.0% |
| ! | TTTGAGGCATAGATTACCGA+TGG | - | tig0024536:78444-78463 | Msa1418150:intron | 40.0% |
| TTGAGGCATAGATTACCGAT+GGG | - | tig0024536:78445-78464 | Msa1418150:intron | 40.0% | |
| CAACAAAGTCAATGAAGCCT+TGG | + | tig0024536:78474-78493 | None:intergenic | 40.0% | |
| ! | TCGTAAGGGTATGATAACCA+GGG | + | tig0024536:78556-78575 | None:intergenic | 40.0% |
| ! | TTCGTAAGGGTATGATAACC+AGG | + | tig0024536:78557-78576 | None:intergenic | 40.0% |
| !! | CAAGATGATATCGGTTCGTA+AGG | + | tig0024536:78571-78590 | None:intergenic | 40.0% |
| TGAGGCATAGATTACCGATG+GGG | - | tig0024536:78446-78465 | Msa1418150:intron | 45.0% | |
| CTTTGTTGCTCATTTGCTCG+AGG | - | tig0024536:78486-78505 | Msa1418150:intron | 45.0% | |
| ATACAGAAGGTCGCTTCTTC+GGG | + | tig0024536:78516-78535 | None:intergenic | 45.0% | |
| GATACAGAAGGTCGCTTCTT+CGG | + | tig0024536:78517-78536 | None:intergenic | 45.0% | |
| ! | CGTAAGGGTATGATAACCAG+GGG | + | tig0024536:78555-78574 | None:intergenic | 45.0% |
| TGAGGCATAGATTACCGATG+GGG | - | tig0024536:78446-78465 | Msa1418150:intron | 45.0% | |
| CTTTGTTGCTCATTTGCTCG+AGG | - | tig0024536:78486-78505 | Msa1418150:intron | 45.0% | |
| ATACAGAAGGTCGCTTCTTC+GGG | + | tig0024536:78516-78535 | None:intergenic | 45.0% | |
| GATACAGAAGGTCGCTTCTT+CGG | + | tig0024536:78517-78536 | None:intergenic | 45.0% | |
| ! | CGTAAGGGTATGATAACCAG+GGG | + | tig0024536:78555-78574 | None:intergenic | 45.0% |
| CAGCACTTGTGAGACTGCTT+GGG | + | tig0024536:78268-78287 | None:intergenic | 50.0% | |
| GAGGCATAGATTACCGATGG+GGG | - | tig0024536:78447-78466 | Msa1418150:intron | 50.0% | |
| CAGCACTTGTGAGACTGCTT+GGG | + | tig0024536:78268-78287 | None:intergenic | 50.0% | |
| GAGGCATAGATTACCGATGG+GGG | - | tig0024536:78447-78466 | Msa1418150:intron | 50.0% | |
| GCAGCACTTGTGAGACTGCT+TGG | + | tig0024536:78269-78288 | None:intergenic | 55.0% | |
| AGATTACCGATGGGGGACCA+AGG | - | tig0024536:78454-78473 | Msa1418150:intron | 55.0% | |
| ATGAAGCCTTGGTCCCCCAT+CGG | + | tig0024536:78463-78482 | None:intergenic | 55.0% | |
| !! | CTCAAGCTCTAAAGCACCCC+TGG | - | tig0024536:78536-78555 | Msa1418150:CDS | 55.0% |
| GCAGCACTTGTGAGACTGCT+TGG | + | tig0024536:78269-78288 | None:intergenic | 55.0% | |
| AGATTACCGATGGGGGACCA+AGG | - | tig0024536:78454-78473 | Msa1418150:intron | 55.0% | |
| ATGAAGCCTTGGTCCCCCAT+CGG | + | tig0024536:78463-78482 | None:intergenic | 55.0% | |
| !! | CTCAAGCTCTAAAGCACCCC+TGG | - | tig0024536:78536-78555 | Msa1418150:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024536 | gene | 77821 | 78591 | 77821 | ID=Msa1418150;Name=Msa1418150 |
| tig0024536 | mRNA | 77821 | 78591 | 77821 | ID=Msa1418150-mRNA-1;Parent=Msa1418150;Name=Msa1418150-mRNA-1;_AED=0.27;_eAED=0.27;_QI=5|1|0.5|1|0|0.5|2|0|85 |
| tig0024536 | exon | 77821 | 78009 | 77821 | ID=Msa1418150-mRNA-1:exon:210;Parent=Msa1418150-mRNA-1 |
| tig0024536 | exon | 78518 | 78591 | 78518 | ID=Msa1418150-mRNA-1:exon:209;Parent=Msa1418150-mRNA-1 |
| tig0024536 | five_prime_UTR | 78587 | 78591 | 78587 | ID=Msa1418150-mRNA-1:five_prime_utr;Parent=Msa1418150-mRNA-1 |
| tig0024536 | CDS | 78518 | 78586 | 78518 | ID=Msa1418150-mRNA-1:cds;Parent=Msa1418150-mRNA-1 |
| tig0024536 | CDS | 77821 | 78009 | 77821 | ID=Msa1418150-mRNA-1:cds;Parent=Msa1418150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
