AlfalfaGEDB | Msa1418290

M. sativa cultivar zhongmu-4 / Msa1418290

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010321 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0048580 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000112 GO:2001141
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418290	sp\|Q38741\|SBP1_ANTMA	60.177	113	41	1	30	142	22	130	8.29e-39	132

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418290	A0A072VA58	94.737	171	9	0	7	177	1	171	4.33e-101	297

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TFs/TRs:

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Gene ID	Type	Classification
Msa1418290	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418290	MtrunA17_Chr2g0317041	91.818	110	9	0	1	110	36	145	1.65e-56	175
Msa1418290	MtrunA17_Chr8g0360361	68.333	120	36	1	60	177	61	180	1.79e-51	163
Msa1418290	MtrunA17_Chr2g0317031	95.522	67	3	0	111	177	9	75	3.00e-42	136
Msa1418290	MtrunA17_Chr2g0282521	60.952	105	39	1	38	140	36	140	3.14e-38	128
Msa1418290	MtrunA17_Chr4g0046991	67.391	92	30	0	48	139	57	148	4.02e-38	128
Msa1418290	MtrunA17_Chr8g0334671	61.765	102	39	0	37	138	160	261	4.76e-36	127
Msa1418290	MtrunA17_Chr3g0122411	64.045	89	32	0	49	137	150	238	8.30e-36	129
Msa1418290	MtrunA17_Chr7g0258471	72.727	77	21	0	61	137	139	215	1.70e-34	125
Msa1418290	MtrunA17_Chr2g0302791	67.442	86	28	0	64	149	139	224	1.73e-34	128
Msa1418290	MtrunA17_Chr7g0270541	67.391	92	30	0	56	147	137	228	3.39e-34	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418290	AT1G53160.3	63.551	107	36	2	50	156	42	145	5.41e-39	131
Msa1418290	AT1G53160.2	65.049	103	33	2	54	156	46	145	9.50e-39	130
Msa1418290	AT1G53160.1	65.049	103	33	2	54	156	46	145	9.50e-39	130
Msa1418290	AT3G15270.1	51.351	148	65	2	7	148	1	147	1.51e-38	130
Msa1418290	AT1G02065.1	61.321	106	37	2	50	151	171	276	9.16e-37	130
Msa1418290	AT2G33810.1	71.250	80	23	0	60	139	50	129	6.82e-36	122
Msa1418290	AT1G69170.1	56.731	104	45	0	34	137	94	197	2.99e-35	127
Msa1418290	AT3G60030.1	62.136	103	34	2	64	166	127	224	1.26e-34	129
Msa1418290	AT1G20980.1	60.784	102	38	1	54	155	112	211	8.57e-34	126
Msa1418290	AT2G42200.1	68.675	83	26	0	62	144	72	154	1.85e-33	122

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Find 36 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATCAGTTGAGAAGGTT+TGG	0.391376	tig0024566:-62202	Msa1418290:CDS
AACCACCACAACAGATGCTT+TGG	0.458273	tig0024566:+61965	None:intergenic
AAGAAAGGTGTTTGTGTGAG+TGG	0.453403	tig0024566:-62110	Msa1418290:CDS
AAGCAAGGAATACAATAGAG+GGG	0.716459	tig0024566:-62229	Msa1418290:CDS
AATAGAAGTTGAAGAAGAGG+AGG	0.552525	tig0024566:-62159	Msa1418290:CDS
AATGAACCTCACAAACCTTA+TGG	0.389353	tig0024566:+61988	None:intergenic
ACTGCTGGAACTTGCACTGA+AGG	0.529046	tig0024566:-59440	Msa1418290:CDS
ACTGGCCAGCAAAATCATGA+TGG	0.639807	tig0024566:-59410	Msa1418290:CDS
AGAAAAGAGCCAAGTAGAGG+TGG	0.571945	tig0024566:-62086	Msa1418290:CDS
AGAAAGGTGTTTGTGTGAGT+GGG	0.554222	tig0024566:-62109	Msa1418290:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAATAGAAGTTGAAGAAG+AGG	-	tig0024566:59414-59433	Msa1418290:CDS	30.0%
CAACTTTCTACTTGACAAAA+AGG	+	tig0024566:59529-59548	None:intergenic	30.0%
TTCATAAGTTTAGATGACAG+TGG	+	tig0024566:59693-59712	None:intergenic	30.0%
TCATCTAAACTTATGAAGCA+CGG	-	tig0024566:59696-59715	Msa1418290:intron	30.0%
AGTTTAGATTCTACGAAATG+CGG	+	tig0024566:59875-59894	None:intergenic	30.0%
AAAATTCTTGGATCCATTAG+TGG	+	tig0024566:60017-60036	None:intergenic	30.0%
TTTGGAAAACCTTTCTTTGT+TGG	+	tig0024566:60514-60533	None:intergenic	30.0%
AAAAATTCTCTCTCATCCAT+AGG	-	tig0024566:60994-61013	Msa1418290:intron	30.0%
ATTTGTTTGAAAAAGGTCCT+TGG	+	tig0024566:61771-61790	None:intergenic	30.0%
TTTCAAATAAGGTGAATGCA+TGG	+	tig0024566:62011-62030	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024566	gene	59343	62255	59343	ID=Msa1418290;Name=Msa1418290
tig0024566	mRNA	59343	62255	59343	ID=Msa1418290-mRNA-1;Parent=Msa1418290;Name=Msa1418290-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|2\|0\|177
tig0024566	exon	61927	62255	61927	ID=Msa1418290-mRNA-1:exon:280;Parent=Msa1418290-mRNA-1
tig0024566	exon	59343	59547	59343	ID=Msa1418290-mRNA-1:exon:279;Parent=Msa1418290-mRNA-1
tig0024566	CDS	61927	62255	61927	ID=Msa1418290-mRNA-1:cds;Parent=Msa1418290-mRNA-1
tig0024566	CDS	59343	59547	59343	ID=Msa1418290-mRNA-1:cds;Parent=Msa1418290-mRNA-1

Gene Sequence

>Msa1418290

ATGGAAGCAAGGAATACAATAGAGGGGAAGCAAACATCAGTTGAGAAGGTTTGGAAAGACATCTTATTTGAAGAAATAGAAGTTGAAGAAGAGGAGGAAGGTGGTGTTGAAGATGATGAGAAGAAGAAAGGTGTTTGTGTGAGTGGGAGAAAAGAGCCAAGTAGAGGTGGAAGATTTTCTCCACCTTTTTGTCAAGTAGAAAGTTGTGGAGCTGATTTAACTTTTGCAAAGAGATATCATCGTCGCCATAAGGTTTGTGAGGTTCATTCCAAAGCATCTGTTGTGGTGGTTGCAGGGATAAGGCAGAGGTTTTGCCAACAATGTAGCAGGTTTCATGAGCTTGCAGAGTTTGATGAATCGAAAAGAAGCTGTCGAAGACGCTTAGCGCGACACAATGAACGGCGTCGTAAAAGCACTGCTGGAACTTGCACTGAAGGAAGCGGTACTGGCCAGCAAAATCATGATGGTGATTGGAGACAAATTCATATGAACATAACAAGTAGTTCTGGTCACGATTTTCTGAATTTCAGATGA

Protein sequence

>Msa1418290

MEARNTIEGKQTSVEKVWKDILFEEIEVEEEEEGGVEDDEKKKGVCVSGRKEPSRGGRFSPPFCQVESCGADLTFAKRYHRRHKVCEVHSKASVVVVAGIRQRFCQQCSRFHELAEFDESKRSCRRRLARHNERRRKSTAGTCTEGSGTGQQNHDGDWRQIHMNITSSSGHDFLNFR*