AlfalfaGEDB | Msa1419410

M. sativa cultivar zhongmu-4 / Msa1419410

KEGG: -
GO: -
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1419410	sp\|G7J1L1\|TF80_MEDTR	66.540	263	14	2	1	189	176	438	1.96e-112	330

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1419410	A0A396IK03	65.779	263	15	3	1	189	91	352	1.21e-107	321

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TFs/TRs:

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Gene ID	Type	Classification
Msa1419410	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1419410	MtrunA17_Chr3g0085681	66.540	263	14	2	1	189	176	438	1.45e-113	330
Msa1419410	MtrunA17_Chr3g0085361	65.779	263	15	3	1	189	91	352	2.32e-111	321
Msa1419410	MtrunA17_Chr5g0396491	36.727	275	89	4	1	190	207	481	1.12e-47	162
Msa1419410	MtrunA17_Chr4g0038851	35.185	270	91	3	5	190	203	472	3.37e-46	157
Msa1419410	MtrunA17_Chr3g0087711	86.207	58	8	0	1	58	1	58	1.64e-29	103
Msa1419410	MtrunA17_Chr7g0224271	28.708	209	117	6	5	187	471	673	3.79e-16	75.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1419410	AT1G50420.1	34.799	273	95	5	1	190	210	482	6.38e-44	152
Msa1419410	AT1G66350.1	25.221	226	102	6	9	190	307	509	2.84e-15	73.6
Msa1419410	AT5G48150.1	25.909	220	100	4	12	187	290	490	4.89e-11	61.2
Msa1419410	AT5G48150.4	25.909	220	100	4	12	187	290	490	4.89e-11	61.2
Msa1419410	AT5G48150.2	25.909	220	100	4	12	187	290	490	4.89e-11	61.2
Msa1419410	AT5G48150.3	25.909	220	100	4	12	187	290	490	4.89e-11	61.2

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Find 31 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGTACCTTTGAGTTACAA+GGG	0.614903	tig0024717:+4058	Msa1419410:CDS
AATGGTCTCCTCAGCAGGTG+AGG	0.601907	tig0024717:+3594	Msa1419410:CDS
ACAGCATCTCCTGTCTTGAC+AGG	0.515953	tig0024717:-3520	None:intergenic
AGCTCGAGAAATGGATTCGA+AGG	0.510467	tig0024717:+4014	Msa1419410:CDS
AGGTACCTTTGAGTTACAAG+GGG	0.670763	tig0024717:+4059	Msa1419410:CDS
ATCCTCCCCTTGTAACTCAA+AGG	0.581446	tig0024717:-4064	None:intergenic
ATGATTGTTTGCTTGTCTGC+TGG	0.372800	tig0024717:+4143	Msa1419410:CDS
ATGCAGAGAGCAGCGCACAT+GGG	0.647261	tig0024717:+3628	Msa1419410:CDS
ATTATTGGATTGAACTGCAA+AGG	0.517946	tig0024717:-3463	None:intergenic
ATTCGAAGGCTTGAAATGAA+TGG	0.452467	tig0024717:+4028	Msa1419410:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTATTGGATTGAACTGCAA+AGG	-	tig0024717:3466-3485	None:intergenic	30.0%
AAGAGGTGATAATTCTGAAT+CGG	-	tig0024717:3711-3730	None:intergenic	30.0%
TTCAGAAAAATCTCCATCTT+AGG	-	tig0024717:3749-3768	None:intergenic	30.0%
AAAGAACATTATTACCTGTG+TGG	+	tig0024717:3964-3983	Msa1419410:intron	30.0%
AAGAACATTATTACCTGTGT+GGG	+	tig0024717:3965-3984	Msa1419410:intron	30.0%
ATTATTGGATTGAACTGCAA+AGG	-	tig0024717:3466-3485	None:intergenic	30.0%
AAGAGGTGATAATTCTGAAT+CGG	-	tig0024717:3711-3730	None:intergenic	30.0%
TTCAGAAAAATCTCCATCTT+AGG	-	tig0024717:3749-3768	None:intergenic	30.0%
AAAGAACATTATTACCTGTG+TGG	+	tig0024717:3964-3983	Msa1419410:intron	30.0%
AAGAACATTATTACCTGTGT+GGG	+	tig0024717:3965-3984	Msa1419410:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024717	gene	3421	4213	3421	ID=Msa1419410;Name=Msa1419410
tig0024717	mRNA	3421	4213	3421	ID=Msa1419410-mRNA-1;Parent=Msa1419410;Name=Msa1419410-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|1\|1\|1\|3\|0\|190
tig0024717	exon	3421	3649	3421	ID=Msa1419410-mRNA-1:exon:886;Parent=Msa1419410-mRNA-1
tig0024717	exon	3738	3864	3738	ID=Msa1419410-mRNA-1:exon:887;Parent=Msa1419410-mRNA-1
tig0024717	exon	3997	4213	3997	ID=Msa1419410-mRNA-1:exon:888;Parent=Msa1419410-mRNA-1
tig0024717	CDS	3421	3649	3421	ID=Msa1419410-mRNA-1:cds;Parent=Msa1419410-mRNA-1
tig0024717	CDS	3738	3864	3738	ID=Msa1419410-mRNA-1:cds;Parent=Msa1419410-mRNA-1
tig0024717	CDS	3997	4213	3997	ID=Msa1419410-mRNA-1:cds;Parent=Msa1419410-mRNA-1

Gene Sequence

>Msa1419410

ATGAGCTTTCATTTGACAGCTGAAGCAGGGATTTTGGATTTTCCTTTGCAGTTCAATCCAATAATTAGCAAACTTGAAGATGTCGATTTCGAAAACTTGCCTGTCAAGACAGGAGATGCTGTTGCAATTTCTTCTGTTCTTCAGCTGCATTCTCTCCTTGCCACTGATGACGAAATGGTCTCCTCAGCAGGTGAGGCATCTTTCAATATGCAGAGAGCAGCGCACATGGGTGCTTCACCTAAGATGGAGATTTTTCTGAACGCTATGCGAAAACTACAACCGAAACTTTTTGTGATTACTGAACAGGAATCAAATCTGAATGGATGTAATTTAATGGAGAGAATCGATCGAGCATTAAAGGAAAGACATGAGAAGCTCGAGAAATGGATTCGAAGGCTTGAAATGAATGGATTTGTGAAGGTACCTTTGAGTTACAAGGGGAGGATAGAAGCAACTAATCTATTGCAGCGATATAGTCACAAGTACAAGTTTAAAGAAGAACATGATTGTTTGCTTGTCTGCTGGAGTGACAGACCACAGTTTTCTGTATCGGCCTGGAAATTTAGGAGATGA

Protein sequence

>Msa1419410

MSFHLTAEAGILDFPLQFNPIISKLEDVDFENLPVKTGDAVAISSVLQLHSLLATDDEMVSSAGEASFNMQRAAHMGASPKMEIFLNAMRKLQPKLFVITEQESNLNGCNLMERIDRALKERHEKLEKWIRRLEMNGFVKVPLSYKGRIEATNLLQRYSHKYKFKEEHDCLLVCWSDRPQFSVSAWKFRR*