Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419410 | sp|G7J1L1|TF80_MEDTR | 66.540 | 263 | 14 | 2 | 1 | 189 | 176 | 438 | 1.96e-112 | 330 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419410 | A0A396IK03 | 65.779 | 263 | 15 | 3 | 1 | 189 | 91 | 352 | 1.21e-107 | 321 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1419410 | TF | GRAS |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419410 | MtrunA17_Chr3g0085681 | 66.540 | 263 | 14 | 2 | 1 | 189 | 176 | 438 | 1.45e-113 | 330 |
| Msa1419410 | MtrunA17_Chr3g0085361 | 65.779 | 263 | 15 | 3 | 1 | 189 | 91 | 352 | 2.32e-111 | 321 |
| Msa1419410 | MtrunA17_Chr5g0396491 | 36.727 | 275 | 89 | 4 | 1 | 190 | 207 | 481 | 1.12e-47 | 162 |
| Msa1419410 | MtrunA17_Chr4g0038851 | 35.185 | 270 | 91 | 3 | 5 | 190 | 203 | 472 | 3.37e-46 | 157 |
| Msa1419410 | MtrunA17_Chr3g0087711 | 86.207 | 58 | 8 | 0 | 1 | 58 | 1 | 58 | 1.64e-29 | 103 |
| Msa1419410 | MtrunA17_Chr7g0224271 | 28.708 | 209 | 117 | 6 | 5 | 187 | 471 | 673 | 3.79e-16 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419410 | AT1G50420.1 | 34.799 | 273 | 95 | 5 | 1 | 190 | 210 | 482 | 6.38e-44 | 152 |
| Msa1419410 | AT1G66350.1 | 25.221 | 226 | 102 | 6 | 9 | 190 | 307 | 509 | 2.84e-15 | 73.6 |
| Msa1419410 | AT5G48150.1 | 25.909 | 220 | 100 | 4 | 12 | 187 | 290 | 490 | 4.89e-11 | 61.2 |
| Msa1419410 | AT5G48150.4 | 25.909 | 220 | 100 | 4 | 12 | 187 | 290 | 490 | 4.89e-11 | 61.2 |
| Msa1419410 | AT5G48150.2 | 25.909 | 220 | 100 | 4 | 12 | 187 | 290 | 490 | 4.89e-11 | 61.2 |
| Msa1419410 | AT5G48150.3 | 25.909 | 220 | 100 | 4 | 12 | 187 | 290 | 490 | 4.89e-11 | 61.2 |
Find 31 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAAGTTTGCTAATTAT+TGG | 0.118308 | tig0024717:-3478 | None:intergenic |
| CTGTATCGGCCTGGAAATTT+AGG | 0.200404 | tig0024717:+4185 | Msa1419410:CDS |
| GACAGCTGAAGCAGGGATTT+TGG | 0.213283 | tig0024717:+3435 | Msa1419410:CDS |
| TCTGAATGGATGTAATTTAA+TGG | 0.250687 | tig0024717:+3823 | Msa1419410:CDS |
| ATGATTGTTTGCTTGTCTGC+TGG | 0.372800 | tig0024717:+4143 | Msa1419410:CDS |
| TAATCATCTCCTAAATTTCC+AGG | 0.378891 | tig0024717:-4194 | None:intergenic |
| TTTAGGTGCTTCACCTAAGA+TGG | 0.394849 | tig0024717:+3733 | Msa1419410:intron |
| GACATGAGAAGCTCGAGAAA+TGG | 0.402484 | tig0024717:+4005 | Msa1419410:CDS |
| CCTTGCCACTGATGACGAAA+TGG | 0.436802 | tig0024717:+3576 | Msa1419410:CDS |
| ATTCGAAGGCTTGAAATGAA+TGG | 0.452467 | tig0024717:+4028 | Msa1419410:CDS |
| GACGAAATGGTCTCCTCAGC+AGG | 0.469157 | tig0024717:+3589 | Msa1419410:CDS |
| TTTCATTTGACAGCTGAAGC+AGG | 0.472492 | tig0024717:+3427 | Msa1419410:CDS |
| GAAAACTTGCCTGTCAAGAC+AGG | 0.495316 | tig0024717:+3511 | Msa1419410:CDS |
| GAACAGGAATCAAATCTGAA+TGG | 0.501626 | tig0024717:+3809 | Msa1419410:CDS |
| AGCTCGAGAAATGGATTCGA+AGG | 0.510467 | tig0024717:+4014 | Msa1419410:CDS |
| ACAGCATCTCCTGTCTTGAC+AGG | 0.515953 | tig0024717:-3520 | None:intergenic |
| ATTATTGGATTGAACTGCAA+AGG | 0.517946 | tig0024717:-3463 | None:intergenic |
| TTCATTTGACAGCTGAAGCA+GGG | 0.544408 | tig0024717:+3428 | Msa1419410:CDS |
| GAAGGTACCTTTGAGTTACA+AGG | 0.545129 | tig0024717:+4057 | Msa1419410:CDS |
| TACCTTTGAGTTACAAGGGG+AGG | 0.564683 | tig0024717:+4062 | Msa1419410:CDS |
| TATGCAGAGAGCAGCGCACA+TGG | 0.577233 | tig0024717:+3627 | Msa1419410:CDS |
| ATCCTCCCCTTGTAACTCAA+AGG | 0.581446 | tig0024717:-4064 | None:intergenic |
| GAAAGATGCCTCACCTGCTG+AGG | 0.590092 | tig0024717:-3602 | None:intergenic |
| AATGGTCTCCTCAGCAGGTG+AGG | 0.601907 | tig0024717:+3594 | Msa1419410:CDS |
| CCATTTCGTCATCAGTGGCA+AGG | 0.614165 | tig0024717:-3576 | None:intergenic |
| AAGGTACCTTTGAGTTACAA+GGG | 0.614903 | tig0024717:+4058 | Msa1419410:CDS |
| TGAAATGAATGGATTTGTGA+AGG | 0.615702 | tig0024717:+4039 | Msa1419410:CDS |
| GGAGACCATTTCGTCATCAG+TGG | 0.638809 | tig0024717:-3581 | None:intergenic |
| ATGCAGAGAGCAGCGCACAT+GGG | 0.647261 | tig0024717:+3628 | Msa1419410:CDS |
| AGGTACCTTTGAGTTACAAG+GGG | 0.670763 | tig0024717:+4059 | Msa1419410:CDS |
| CAGGCCGATACAGAAAACTG+TGG | 0.752207 | tig0024717:-4175 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTCAAGTTTGCTAATTAT+TGG | - | tig0024717:3481-3500 | None:intergenic | 25.0% |
| !!! | GAATTATCACCTCTTTTTTT+AGG | + | tig0024717:3716-3735 | Msa1419410:intron | 25.0% |
| ! | TCAGTAATCACAAAAAGTTT+CGG | - | tig0024717:3791-3810 | None:intergenic | 25.0% |
| ! | TCTGAATGGATGTAATTTAA+TGG | + | tig0024717:3823-3842 | Msa1419410:CDS | 25.0% |
| !!! | TCTTCAAGTTTGCTAATTAT+TGG | - | tig0024717:3481-3500 | None:intergenic | 25.0% |
| !!! | GAATTATCACCTCTTTTTTT+AGG | + | tig0024717:3716-3735 | Msa1419410:intron | 25.0% |
| ! | TCAGTAATCACAAAAAGTTT+CGG | - | tig0024717:3791-3810 | None:intergenic | 25.0% |
| ! | TCTGAATGGATGTAATTTAA+TGG | + | tig0024717:3823-3842 | Msa1419410:CDS | 25.0% |
| ATTATTGGATTGAACTGCAA+AGG | - | tig0024717:3466-3485 | None:intergenic | 30.0% | |
| AAGAGGTGATAATTCTGAAT+CGG | - | tig0024717:3711-3730 | None:intergenic | 30.0% | |
| TTCAGAAAAATCTCCATCTT+AGG | - | tig0024717:3749-3768 | None:intergenic | 30.0% | |
| ! | ACTTTTTGTGATTACTGAAC+AGG | + | tig0024717:3793-3812 | Msa1419410:CDS | 30.0% |
| AAAGAACATTATTACCTGTG+TGG | + | tig0024717:3964-3983 | Msa1419410:intron | 30.0% | |
| AAGAACATTATTACCTGTGT+GGG | + | tig0024717:3965-3984 | Msa1419410:intron | 30.0% | |
| ! | TGAAATGAATGGATTTGTGA+AGG | + | tig0024717:4039-4058 | Msa1419410:CDS | 30.0% |
| ATTATTGGATTGAACTGCAA+AGG | - | tig0024717:3466-3485 | None:intergenic | 30.0% | |
| AAGAGGTGATAATTCTGAAT+CGG | - | tig0024717:3711-3730 | None:intergenic | 30.0% | |
| TTCAGAAAAATCTCCATCTT+AGG | - | tig0024717:3749-3768 | None:intergenic | 30.0% | |
| ! | ACTTTTTGTGATTACTGAAC+AGG | + | tig0024717:3793-3812 | Msa1419410:CDS | 30.0% |
| AAAGAACATTATTACCTGTG+TGG | + | tig0024717:3964-3983 | Msa1419410:intron | 30.0% | |
| AAGAACATTATTACCTGTGT+GGG | + | tig0024717:3965-3984 | Msa1419410:intron | 30.0% | |
| ! | TGAAATGAATGGATTTGTGA+AGG | + | tig0024717:4039-4058 | Msa1419410:CDS | 30.0% |
| AGAGGTGATAATTCTGAATC+GGG | - | tig0024717:3710-3729 | None:intergenic | 35.0% | |
| GAACAGGAATCAAATCTGAA+TGG | + | tig0024717:3809-3828 | Msa1419410:CDS | 35.0% | |
| !! | GATCGAGCATTGTACTTTTA+CGG | + | tig0024717:3854-3873 | Msa1419410:intron | 35.0% |
| ATTCGAAGGCTTGAAATGAA+TGG | + | tig0024717:4028-4047 | Msa1419410:CDS | 35.0% | |
| ! | AAGGTACCTTTGAGTTACAA+GGG | + | tig0024717:4058-4077 | Msa1419410:CDS | 35.0% |
| AGAGGTGATAATTCTGAATC+GGG | - | tig0024717:3710-3729 | None:intergenic | 35.0% | |
| GAACAGGAATCAAATCTGAA+TGG | + | tig0024717:3809-3828 | Msa1419410:CDS | 35.0% | |
| !! | GATCGAGCATTGTACTTTTA+CGG | + | tig0024717:3854-3873 | Msa1419410:intron | 35.0% |
| ATTCGAAGGCTTGAAATGAA+TGG | + | tig0024717:4028-4047 | Msa1419410:CDS | 35.0% | |
| ! | AAGGTACCTTTGAGTTACAA+GGG | + | tig0024717:4058-4077 | Msa1419410:CDS | 35.0% |
| TTTCATTTGACAGCTGAAGC+AGG | + | tig0024717:3427-3446 | Msa1419410:CDS | 40.0% | |
| TTCATTTGACAGCTGAAGCA+GGG | + | tig0024717:3428-3447 | Msa1419410:CDS | 40.0% | |
| ATAATTCTGAATCGGGACTC+AGG | - | tig0024717:3703-3722 | None:intergenic | 40.0% | |
| ! | GGTGAAGCACCTAAAAAAAG+AGG | - | tig0024717:3728-3747 | None:intergenic | 40.0% |
| TTTAGGTGCTTCACCTAAGA+TGG | + | tig0024717:3733-3752 | Msa1419410:intron | 40.0% | |
| ! | AAACTTGAGAGCATGCTTCT+TGG | + | tig0024717:3932-3951 | Msa1419410:intron | 40.0% |
| !! | GAAGGTACCTTTGAGTTACA+AGG | + | tig0024717:4057-4076 | Msa1419410:CDS | 40.0% |
| ! | AGGTACCTTTGAGTTACAAG+GGG | + | tig0024717:4059-4078 | Msa1419410:CDS | 40.0% |
| ATGATTGTTTGCTTGTCTGC+TGG | + | tig0024717:4143-4162 | Msa1419410:CDS | 40.0% | |
| ! | CAGACCACAGTTTTCTGTAT+CGG | + | tig0024717:4171-4190 | Msa1419410:CDS | 40.0% |
| TTTCATTTGACAGCTGAAGC+AGG | + | tig0024717:3427-3446 | Msa1419410:CDS | 40.0% | |
| TTCATTTGACAGCTGAAGCA+GGG | + | tig0024717:3428-3447 | Msa1419410:CDS | 40.0% | |
| ATAATTCTGAATCGGGACTC+AGG | - | tig0024717:3703-3722 | None:intergenic | 40.0% | |
| ! | GGTGAAGCACCTAAAAAAAG+AGG | - | tig0024717:3728-3747 | None:intergenic | 40.0% |
| TTTAGGTGCTTCACCTAAGA+TGG | + | tig0024717:3733-3752 | Msa1419410:intron | 40.0% | |
| ! | AAACTTGAGAGCATGCTTCT+TGG | + | tig0024717:3932-3951 | Msa1419410:intron | 40.0% |
| !! | GAAGGTACCTTTGAGTTACA+AGG | + | tig0024717:4057-4076 | Msa1419410:CDS | 40.0% |
| ! | AGGTACCTTTGAGTTACAAG+GGG | + | tig0024717:4059-4078 | Msa1419410:CDS | 40.0% |
| ATGATTGTTTGCTTGTCTGC+TGG | + | tig0024717:4143-4162 | Msa1419410:CDS | 40.0% | |
| ! | CAGACCACAGTTTTCTGTAT+CGG | + | tig0024717:4171-4190 | Msa1419410:CDS | 40.0% |
| GAAAACTTGCCTGTCAAGAC+AGG | + | tig0024717:3511-3530 | Msa1419410:CDS | 45.0% | |
| !!! | CGGTTCACTTTTTGACTGCT+TGG | + | tig0024717:3874-3893 | Msa1419410:intron | 45.0% |
| GACATGAGAAGCTCGAGAAA+TGG | + | tig0024717:4005-4024 | Msa1419410:CDS | 45.0% | |
| ! | AGCTCGAGAAATGGATTCGA+AGG | + | tig0024717:4014-4033 | Msa1419410:CDS | 45.0% |
| ! | TACCTTTGAGTTACAAGGGG+AGG | + | tig0024717:4062-4081 | Msa1419410:CDS | 45.0% |
| ATCCTCCCCTTGTAACTCAA+AGG | - | tig0024717:4067-4086 | None:intergenic | 45.0% | |
| ! | CTGTATCGGCCTGGAAATTT+AGG | + | tig0024717:4185-4204 | Msa1419410:CDS | 45.0% |
| GAAAACTTGCCTGTCAAGAC+AGG | + | tig0024717:3511-3530 | Msa1419410:CDS | 45.0% | |
| !!! | CGGTTCACTTTTTGACTGCT+TGG | + | tig0024717:3874-3893 | Msa1419410:intron | 45.0% |
| GACATGAGAAGCTCGAGAAA+TGG | + | tig0024717:4005-4024 | Msa1419410:CDS | 45.0% | |
| ! | AGCTCGAGAAATGGATTCGA+AGG | + | tig0024717:4014-4033 | Msa1419410:CDS | 45.0% |
| ! | TACCTTTGAGTTACAAGGGG+AGG | + | tig0024717:4062-4081 | Msa1419410:CDS | 45.0% |
| ATCCTCCCCTTGTAACTCAA+AGG | - | tig0024717:4067-4086 | None:intergenic | 45.0% | |
| ! | CTGTATCGGCCTGGAAATTT+AGG | + | tig0024717:4185-4204 | Msa1419410:CDS | 45.0% |
| ! | GACAGCTGAAGCAGGGATTT+TGG | + | tig0024717:3435-3454 | Msa1419410:CDS | 50.0% |
| ACAGCATCTCCTGTCTTGAC+AGG | - | tig0024717:3523-3542 | None:intergenic | 50.0% | |
| CCATTTCGTCATCAGTGGCA+AGG | - | tig0024717:3579-3598 | None:intergenic | 50.0% | |
| ! | CCTTGCCACTGATGACGAAA+TGG | + | tig0024717:3576-3595 | Msa1419410:CDS | 50.0% |
| GGAGACCATTTCGTCATCAG+TGG | - | tig0024717:3584-3603 | None:intergenic | 50.0% | |
| !! | CTTTCTATCGACTCCCACAC+AGG | - | tig0024717:3981-4000 | None:intergenic | 50.0% |
| CTGTGTGGGAGTCGATAGAA+AGG | + | tig0024717:3979-3998 | Msa1419410:intron | 50.0% | |
| CAGGCCGATACAGAAAACTG+TGG | - | tig0024717:4178-4197 | None:intergenic | 50.0% | |
| !! | CACAGTTTTCTGTATCGGCC+TGG | + | tig0024717:4176-4195 | Msa1419410:CDS | 50.0% |
| ! | GACAGCTGAAGCAGGGATTT+TGG | + | tig0024717:3435-3454 | Msa1419410:CDS | 50.0% |
| ACAGCATCTCCTGTCTTGAC+AGG | - | tig0024717:3523-3542 | None:intergenic | 50.0% | |
| CCATTTCGTCATCAGTGGCA+AGG | - | tig0024717:3579-3598 | None:intergenic | 50.0% | |
| ! | CCTTGCCACTGATGACGAAA+TGG | + | tig0024717:3576-3595 | Msa1419410:CDS | 50.0% |
| GGAGACCATTTCGTCATCAG+TGG | - | tig0024717:3584-3603 | None:intergenic | 50.0% | |
| !! | CTTTCTATCGACTCCCACAC+AGG | - | tig0024717:3981-4000 | None:intergenic | 50.0% |
| CTGTGTGGGAGTCGATAGAA+AGG | + | tig0024717:3979-3998 | Msa1419410:intron | 50.0% | |
| CAGGCCGATACAGAAAACTG+TGG | - | tig0024717:4178-4197 | None:intergenic | 50.0% | |
| !! | CACAGTTTTCTGTATCGGCC+TGG | + | tig0024717:4176-4195 | Msa1419410:CDS | 50.0% |
| GACGAAATGGTCTCCTCAGC+AGG | + | tig0024717:3589-3608 | Msa1419410:CDS | 55.0% | |
| AATGGTCTCCTCAGCAGGTG+AGG | + | tig0024717:3594-3613 | Msa1419410:CDS | 55.0% | |
| GAAAGATGCCTCACCTGCTG+AGG | - | tig0024717:3605-3624 | None:intergenic | 55.0% | |
| TATGCAGAGAGCAGCGCACA+TGG | + | tig0024717:3627-3646 | Msa1419410:CDS | 55.0% | |
| ATGCAGAGAGCAGCGCACAT+GGG | + | tig0024717:3628-3647 | Msa1419410:CDS | 55.0% | |
| GTCAGAGGACTTTCGCAGAG+TGG | + | tig0024717:3650-3669 | Msa1419410:intron | 55.0% | |
| GACGAAATGGTCTCCTCAGC+AGG | + | tig0024717:3589-3608 | Msa1419410:CDS | 55.0% | |
| AATGGTCTCCTCAGCAGGTG+AGG | + | tig0024717:3594-3613 | Msa1419410:CDS | 55.0% | |
| GAAAGATGCCTCACCTGCTG+AGG | - | tig0024717:3605-3624 | None:intergenic | 55.0% | |
| TATGCAGAGAGCAGCGCACA+TGG | + | tig0024717:3627-3646 | Msa1419410:CDS | 55.0% | |
| ATGCAGAGAGCAGCGCACAT+GGG | + | tig0024717:3628-3647 | Msa1419410:CDS | 55.0% | |
| GTCAGAGGACTTTCGCAGAG+TGG | + | tig0024717:3650-3669 | Msa1419410:intron | 55.0% | |
| GAGCAGCGCACATGGGTCAG+AGG | + | tig0024717:3635-3654 | Msa1419410:intron | 65.0% | |
| GAGCAGCGCACATGGGTCAG+AGG | + | tig0024717:3635-3654 | Msa1419410:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024717 | gene | 3421 | 4213 | 3421 | ID=Msa1419410;Name=Msa1419410 |
| tig0024717 | mRNA | 3421 | 4213 | 3421 | ID=Msa1419410-mRNA-1;Parent=Msa1419410;Name=Msa1419410-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|190 |
| tig0024717 | exon | 3421 | 3649 | 3421 | ID=Msa1419410-mRNA-1:exon:886;Parent=Msa1419410-mRNA-1 |
| tig0024717 | exon | 3738 | 3864 | 3738 | ID=Msa1419410-mRNA-1:exon:887;Parent=Msa1419410-mRNA-1 |
| tig0024717 | exon | 3997 | 4213 | 3997 | ID=Msa1419410-mRNA-1:exon:888;Parent=Msa1419410-mRNA-1 |
| tig0024717 | CDS | 3421 | 3649 | 3421 | ID=Msa1419410-mRNA-1:cds;Parent=Msa1419410-mRNA-1 |
| tig0024717 | CDS | 3738 | 3864 | 3738 | ID=Msa1419410-mRNA-1:cds;Parent=Msa1419410-mRNA-1 |
| tig0024717 | CDS | 3997 | 4213 | 3997 | ID=Msa1419410-mRNA-1:cds;Parent=Msa1419410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
