AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1421340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421340 MtrunA17_Chr8g0360761 58.333 156 48 4 10 165 48 186 9.60e-51 161
Msa1421340 MtrunA17_Chr4g0051261 45.263 190 70 9 1 164 107 288 8.06e-39 133
Msa1421340 MtrunA17_Chr2g0293491 43.158 190 74 9 1 164 107 288 1.18e-36 128
Msa1421340 MtrunA17_Chr8g0358921 39.037 187 84 8 1 163 155 335 4.88e-30 112
Msa1421340 MtrunA17_Chr4g0002001 36.979 192 80 6 1 163 124 303 2.22e-25 99.0
Msa1421340 MtrunA17_Chr1g0187371 37.017 181 85 7 7 163 133 308 2.98e-25 98.6
Msa1421340 MtrunA17_Chr6g0483151 35.135 185 87 7 4 163 118 294 9.50e-23 93.2
Msa1421340 MtrunA17_Chr3g0099951 32.787 183 92 6 5 163 121 296 4.37e-21 88.6
Msa1421340 MtrunA17_Chr4g0057791 34.872 195 90 9 1 164 324 512 1.51e-20 87.4
Msa1421340 MtrunA17_Chr3g0093391 33.673 196 89 8 1 166 141 325 5.78e-20 85.9
Msa1421340 MtrunA17_Chr7g0248461 35.938 192 83 11 1 163 144 324 4.69e-19 82.8
Msa1421340 MtrunA17_Chr8g0387401 30.144 209 80 7 1 167 158 342 4.27e-17 77.4
Msa1421340 MtrunA17_Chr4g0045841 31.443 194 88 9 1 163 161 340 1.75e-16 75.5
Msa1421340 MtrunA17_Chr1g0203881 31.548 168 82 6 12 166 155 302 7.02e-16 73.9
Msa1421340 MtrunA17_Chr4g0030231 30.851 188 98 7 1 163 146 326 8.76e-16 73.6
Msa1421340 MtrunA17_Chr5g0397261 33.508 191 89 9 1 163 147 327 9.61e-16 73.6
Msa1421340 MtrunA17_Chr3g0130781 30.198 202 95 7 7 166 134 331 1.08e-15 73.6
Msa1421340 MtrunA17_Chr8g0348481 31.053 190 95 8 1 163 143 323 1.47e-15 72.8
Msa1421340 MtrunA17_Chr3g0141871 32.828 198 82 8 1 163 138 319 1.65e-15 72.8
Msa1421340 MtrunA17_Chr8g0378831 31.980 197 85 9 1 163 138 319 2.24e-15 72.4
Msa1421340 MtrunA17_Chr3g0102571 32.222 180 95 8 7 163 191 366 3.95e-15 71.6
Msa1421340 MtrunA17_Chr4g0061141 29.167 192 98 10 1 164 209 390 3.04e-14 69.3
Msa1421340 MtrunA17_Chr5g0402551 30.682 176 88 9 2 164 243 397 3.75e-14 69.3
Msa1421340 MtrunA17_Chr4g0065851 29.231 195 97 7 1 163 131 316 5.73e-14 68.6
Msa1421340 MtrunA17_Chr6g0453711 40.860 93 34 4 1 78 214 300 6.54e-14 68.6
Msa1421340 MtrunA17_Chr8g0374701 30.357 168 84 8 10 164 244 391 1.29e-13 67.8
Msa1421340 MtrunA17_Chr4g0045261 30.539 167 85 6 10 164 253 400 1.82e-13 67.0
Msa1421340 MtrunA17_Chr1g0173541 31.737 167 77 9 13 164 167 311 1.89e-13 67.0
Msa1421340 MtrunA17_Chr2g0324461 42.000 100 36 6 1 85 245 337 2.96e-13 66.6
Msa1421340 MtrunA17_Chr7g0273601 30.366 191 95 10 1 163 211 391 7.17e-13 65.5
Msa1421340 MtrunA17_Chr7g0256001 31.953 169 84 7 7 163 254 403 9.29e-13 65.1
Msa1421340 MtrunA17_Chr2g0287271 28.643 199 95 8 1 164 131 317 1.12e-12 64.7
Msa1421340 MtrunA17_Chr5g0402601 28.804 184 84 9 1 164 478 634 1.72e-12 64.3
Msa1421340 MtrunA17_Chr7g0218071 40.860 93 34 4 1 78 224 310 2.04e-12 64.3
Msa1421340 MtrunA17_Chr7g0274941 29.310 174 82 8 7 163 125 274 2.81e-12 63.2
Msa1421340 MtrunA17_Chr1g0202831 29.213 178 90 6 4 168 518 672 3.33e-12 63.5
Msa1421340 MtrunA17_Chr7g0257751 31.737 167 75 8 13 163 173 316 3.78e-12 63.2
Msa1421340 MtrunA17_Chr1g0194201 28.866 194 83 10 7 163 184 359 3.99e-12 63.2
Msa1421340 MtrunA17_Chr1g0191221 30.457 197 93 11 1 166 226 409 4.09e-12 63.2
Msa1421340 MtrunA17_Chr3g0131861 39.785 93 35 5 1 78 201 287 4.82e-12 63.2
Msa1421340 MtrunA17_Chr7g0270371 28.796 191 81 10 9 162 186 358 5.64e-12 62.8
Msa1421340 MtrunA17_Chr8g0385971 39.785 93 35 5 1 78 194 280 7.39e-12 62.4
Msa1421340 MtrunA17_Chr6g0482681 40.659 91 40 3 1 78 195 284 8.85e-12 62.4
Msa1421340 MtrunA17_Chr8g0386981 28.814 177 89 7 1 164 513 665 1.10e-11 62.0
Msa1421340 MtrunA17_Chr4g0051191 31.250 176 85 8 1 164 267 418 1.28e-11 61.6
Msa1421340 MtrunA17_Chr8g0369241 39.130 92 36 4 1 78 246 331 1.31e-11 62.0
Msa1421340 MtrunA17_Chr2g0317501 39.785 93 35 5 1 78 238 324 1.35e-11 62.0
Msa1421340 MtrunA17_Chr4g0040141 38.710 93 36 5 1 78 233 319 1.38e-11 61.6
Msa1421340 MtrunA17_Chr4g0059951 32.530 166 77 8 13 164 188 332 1.64e-11 61.6
Msa1421340 MtrunA17_Chr1g0157131 33.533 167 74 9 13 164 189 333 1.65e-11 61.6
Msa1421340 MtrunA17_Chr4g0030241 39.785 93 37 4 1 80 7 93 1.81e-11 59.7
Msa1421340 MtrunA17_Chr3g0130151 27.684 177 91 7 1 164 349 501 1.90e-11 61.2
Msa1421340 MtrunA17_Chr1g0201571 37.634 93 37 5 1 78 230 316 3.60e-11 60.5
Msa1421340 MtrunA17_Chr1g0205931 26.776 183 95 7 1 167 163 322 4.09e-11 60.5
Msa1421340 MtrunA17_Chr2g0284091 30.000 190 70 9 9 157 151 318 5.08e-11 60.1
Msa1421340 MtrunA17_Chr2g0320011 29.474 190 71 9 9 157 199 366 5.19e-11 60.1
Msa1421340 MtrunA17_Chr4g0033371 29.268 164 85 7 13 164 187 331 7.05e-11 59.7
Msa1421340 MtrunA17_Chr1g0193081 27.979 193 82 9 9 163 186 359 7.66e-11 59.3
Msa1421340 MtrunA17_Chr4g0066691 36.559 93 38 5 1 78 208 294 7.72e-11 59.7
Msa1421340 MtrunA17_Chr8g0383451 36.559 93 38 5 1 78 102 188 9.01e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421340 AT3G48750.1 41.270 189 79 8 1 164 107 288 5.64e-34 121
Msa1421340 AT1G76540.1 38.587 184 78 8 7 163 129 304 6.89e-27 103
Msa1421340 AT1G20930.1 38.122 181 83 7 7 163 131 306 8.48e-26 100
Msa1421340 AT1G18040.1 34.595 185 86 8 5 163 117 292 2.66e-25 100
Msa1421340 AT2G38620.4 35.676 185 92 6 1 163 62 241 3.26e-25 97.8
Msa1421340 AT2G38620.2 35.676 185 92 6 1 163 124 303 9.84e-25 97.8
Msa1421340 AT3G54180.1 36.264 182 89 6 5 163 124 301 2.36e-24 96.7
Msa1421340 AT1G73690.1 34.409 186 87 8 4 163 115 291 1.46e-22 92.8
Msa1421340 AT2G46070.1 35.567 194 90 9 1 167 146 331 2.67e-22 92.0
Msa1421340 AT2G46070.3 35.567 194 90 9 1 167 180 365 3.50e-22 92.0
Msa1421340 AT1G66750.1 34.426 183 89 6 5 163 118 293 2.24e-21 89.4
Msa1421340 AT5G10270.1 34.555 191 94 7 1 166 144 328 2.91e-20 87.0
Msa1421340 AT5G64960.1 32.143 196 92 8 1 166 144 328 9.55e-20 85.5
Msa1421340 AT4G01370.1 35.789 190 86 10 1 163 149 329 2.87e-19 83.6
Msa1421340 AT1G01560.2 34.737 190 88 9 1 163 146 326 4.34e-19 83.2
Msa1421340 AT5G64960.2 32.461 191 98 7 1 166 91 275 6.70e-19 83.2
Msa1421340 AT2G46070.2 35.000 180 82 9 1 153 146 317 2.61e-18 80.5
Msa1421340 AT1G07880.2 32.979 188 94 8 1 163 139 319 9.42e-18 79.3
Msa1421340 AT5G63610.1 32.673 202 90 8 7 166 139 336 9.22e-17 77.0
Msa1421340 AT1G01560.3 33.684 190 89 10 1 163 146 325 9.45e-17 76.3
Msa1421340 AT1G03740.2 31.313 198 86 10 1 164 317 498 5.73e-16 74.7
Msa1421340 AT1G03740.1 31.313 198 86 10 1 164 317 498 6.32e-16 74.7
Msa1421340 AT3G04530.1 32.000 175 85 8 1 163 117 269 3.09e-15 71.6
Msa1421340 AT2G38620.3 43.820 89 37 3 1 78 124 210 4.70e-15 70.9
Msa1421340 AT2G38620.1 43.820 89 37 3 1 78 124 210 4.70e-15 70.9
Msa1421340 AT5G19450.2 35.119 168 70 8 13 164 172 316 7.41e-15 71.6
Msa1421340 AT5G19450.1 35.119 168 70 8 13 164 172 316 7.41e-15 71.6
Msa1421340 AT1G08650.1 28.342 187 79 6 1 164 120 274 1.27e-14 70.1
Msa1421340 AT1G33770.1 43.011 93 32 5 1 78 245 331 1.38e-14 70.9
Msa1421340 AT1G53570.6 30.508 177 86 7 1 164 319 471 2.41e-14 70.1
Msa1421340 AT1G53570.7 30.508 177 86 7 1 164 319 471 2.41e-14 70.1
Msa1421340 AT1G53570.1 30.508 177 86 7 1 164 319 471 4.84e-14 69.3
Msa1421340 AT1G53570.4 30.508 177 86 7 1 164 319 471 4.88e-14 69.3
Msa1421340 AT1G53570.2 30.508 177 86 7 1 164 319 471 5.02e-14 69.3
Msa1421340 AT1G53570.3 30.508 177 86 7 1 164 319 471 5.02e-14 69.3
Msa1421340 AT5G19010.1 30.052 193 100 6 1 164 131 317 8.39e-14 68.6
Msa1421340 AT2G43790.1 32.124 193 96 9 1 167 169 352 8.87e-14 68.2
Msa1421340 AT4G04740.4 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT4G04740.6 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT4G04740.5 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT4G04740.3 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT4G04740.1 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT4G04740.7 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT4G04740.2 35.152 165 74 9 13 164 184 328 1.32e-13 67.8
Msa1421340 AT1G09000.2 28.962 183 90 7 1 167 177 335 1.81e-13 67.8
Msa1421340 AT1G09000.1 28.962 183 90 7 1 167 177 335 2.10e-13 67.4
Msa1421340 AT1G49580.1 29.762 168 85 7 10 164 266 413 2.20e-13 67.4
Msa1421340 AT1G08650.2 30.409 171 84 7 1 158 120 268 2.46e-13 66.2
Msa1421340 AT2G01450.3 27.660 188 111 5 1 164 122 308 3.51e-13 66.6
Msa1421340 AT2G01450.1 27.660 188 111 5 1 164 122 308 3.51e-13 66.6
Msa1421340 AT2G01450.2 27.660 188 111 5 1 164 122 308 3.51e-13 66.6
Msa1421340 AT2G01450.4 27.660 188 111 5 1 164 122 308 3.51e-13 66.6
Msa1421340 AT4G36450.1 30.208 192 94 8 1 164 138 317 4.21e-13 66.2
Msa1421340 AT3G01085.1 31.088 193 92 10 1 163 219 400 5.31e-13 66.2
Msa1421340 AT3G01085.2 31.088 193 92 10 1 163 219 400 5.31e-13 66.2
Msa1421340 AT3G01085.3 31.088 193 92 10 1 163 219 400 5.32e-13 66.2
Msa1421340 AT3G19100.1 29.341 167 85 7 10 163 260 406 7.43e-13 65.9
Msa1421340 AT1G61950.1 31.928 166 79 7 13 164 213 358 7.88e-13 65.9
Msa1421340 AT1G71530.2 28.796 191 104 7 1 166 251 434 9.20e-13 65.5
Msa1421340 AT3G50530.1 29.167 168 86 7 10 164 264 411 1.06e-12 65.5
Msa1421340 AT1G71530.1 29.082 196 91 9 1 163 251 431 1.07e-12 65.5
Msa1421340 AT4G22940.1 28.856 201 91 9 1 166 208 391 1.28e-12 65.1
Msa1421340 AT4G22940.2 28.856 201 91 9 1 166 208 391 1.37e-12 65.1
Msa1421340 AT4G13020.5 28.571 189 103 8 1 165 114 294 2.46e-12 64.3
Msa1421340 AT4G13020.1 28.571 189 103 8 1 165 105 285 2.71e-12 63.9
Msa1421340 AT4G13020.2 28.571 189 103 8 1 165 113 293 2.76e-12 63.9
Msa1421340 AT4G13020.3 28.571 189 103 8 1 165 113 293 2.76e-12 63.9
Msa1421340 AT2G41860.1 31.928 166 78 8 13 164 169 313 2.80e-12 64.3
Msa1421340 AT2G41860.2 31.928 166 78 8 13 164 169 313 2.80e-12 64.3
Msa1421340 AT1G71530.3 29.255 188 101 7 1 163 251 431 2.82e-12 63.9
Msa1421340 AT4G13020.6 28.571 189 103 8 1 165 105 285 2.98e-12 63.9
Msa1421340 AT4G13020.4 28.571 189 103 8 1 165 105 285 3.03e-12 63.9
Msa1421340 AT4G13020.7 28.571 189 103 8 1 165 105 285 3.74e-12 63.5
Msa1421340 AT4G13020.8 28.571 189 103 8 1 165 114 294 3.84e-12 63.5
Msa1421340 AT5G50860.1 39.785 93 35 5 1 78 218 304 4.65e-12 63.5
Msa1421340 AT4G10010.2 28.788 198 95 11 1 166 260 443 5.19e-12 63.5
Msa1421340 AT1G54960.1 27.374 179 90 7 1 163 176 330 5.29e-12 63.5
Msa1421340 AT1G54960.2 27.374 179 90 7 1 163 176 330 5.29e-12 63.5
Msa1421340 AT5G39440.1 29.143 175 85 6 2 163 122 270 5.53e-12 63.2
Msa1421340 AT5G12480.2 32.335 167 74 9 13 163 80 223 5.60e-12 63.2
Msa1421340 AT5G12480.1 31.737 167 75 8 13 163 174 317 6.39e-12 63.2
Msa1421340 AT3G57530.2 31.548 168 82 7 10 164 175 322 7.45e-12 62.8
Msa1421340 AT3G57530.1 32.317 164 80 7 13 164 178 322 8.05e-12 62.8
Msa1421340 AT5G50860.2 39.785 93 35 5 1 78 218 304 9.02e-12 62.8
Msa1421340 AT1G18670.1 38.889 90 41 5 1 78 235 322 1.01e-11 62.8
Msa1421340 AT1G18670.3 38.889 90 41 5 1 78 235 322 1.12e-11 62.4
Msa1421340 AT1G18670.2 38.889 90 41 5 1 78 235 322 1.12e-11 62.4
Msa1421340 AT4G19110.2 27.273 187 91 8 9 164 112 284 1.24e-11 62.4
Msa1421340 AT4G19110.1 27.273 187 91 8 9 164 112 284 1.27e-11 62.0
Msa1421340 AT4G19110.3 27.273 187 91 8 9 164 56 228 1.30e-11 62.0
Msa1421340 AT1G53570.5 29.878 164 78 6 1 164 319 445 1.35e-11 62.0
Msa1421340 AT2G31500.1 28.743 167 82 7 13 164 181 325 1.44e-11 62.0
Msa1421340 AT4G10010.1 28.788 198 95 11 1 166 80 263 1.66e-11 62.0
Msa1421340 AT4G21940.1 33.333 165 77 9 13 164 217 361 1.71e-11 62.0
Msa1421340 AT4G21940.2 33.333 165 77 9 13 164 217 361 1.72e-11 62.0
Msa1421340 AT3G06030.1 27.374 179 90 7 1 163 176 330 1.79e-11 62.0
Msa1421340 AT4G29810.1 32.022 178 86 10 2 163 172 330 2.15e-11 61.2
Msa1421340 AT5G14640.1 28.497 193 81 10 9 163 185 358 2.43e-11 61.2
Msa1421340 AT5G14640.2 28.497 193 81 10 9 163 185 358 2.43e-11 61.2
Msa1421340 AT4G21940.3 33.939 165 76 10 13 164 217 361 2.51e-11 61.2
Msa1421340 AT4G29810.2 32.022 178 86 10 2 163 181 339 2.62e-11 61.2
Msa1421340 AT1G63700.1 29.885 174 86 6 4 164 507 657 2.64e-11 61.2
Msa1421340 AT1G63700.2 29.885 174 86 6 4 164 507 657 2.64e-11 61.2
Msa1421340 AT1G01560.4 41.758 91 34 4 1 78 146 230 3.33e-11 60.5
Msa1421340 AT1G01560.1 41.758 91 34 4 1 78 146 230 3.33e-11 60.5
Msa1421340 AT1G06390.2 29.474 190 71 10 9 157 181 348 4.80e-11 60.5
Msa1421340 AT1G06390.1 29.474 190 71 10 9 157 181 348 4.80e-11 60.5
Msa1421340 AT1G53050.2 39.785 93 35 5 1 78 238 324 5.86e-11 60.5
Msa1421340 AT1G53050.1 39.785 93 35 5 1 78 238 324 5.86e-11 60.5
Msa1421340 AT1G71530.4 38.710 93 36 4 1 78 251 337 8.82e-11 59.7

Find 35 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTGAAATCCTGCTATT+TGG 0.152353 tig0024927:+71556 None:intergenic
AGATATTGGCTGAAATATTT+TGG 0.170047 tig0024927:-72121 Msa1421340:intron
GAAATTATCTGACTTTAATT+TGG 0.188343 tig0024927:-72444 Msa1421340:intron
TGCAGTTGATATCTGGTCTA+TGG 0.259046 tig0024927:-72194 Msa1421340:CDS
ACTGAAGCCAGAATATTATT+AGG 0.269823 tig0024927:+72491 None:intergenic
ATCAACTGCAGTTGAGTAAT+TGG 0.289792 tig0024927:+72207 None:intergenic
CAGCTGAAATCCTGCTATTT+GGG 0.318029 tig0024927:+71557 None:intergenic
TCTGAAACCTAATAATATTC+TGG 0.322462 tig0024927:-72498 Msa1421340:CDS
ATGAATACCTTTGGCATGAT+TGG 0.339803 tig0024927:+71919 None:intergenic
TAAAGATGAACCGCAGATAT+TGG 0.377267 tig0024927:-72135 Msa1421340:CDS
ACTCAACTGCAGTTGATATC+TGG 0.396574 tig0024927:-72201 Msa1421340:CDS
AGGCTCCAACCTCTGTTCAT+TGG 0.404026 tig0024927:-72158 Msa1421340:CDS
GAAGAACCCATCAAAAGTTC+AGG 0.440653 tig0024927:+72232 None:intergenic
AGACCATCTGCTTCGAGCCT+AGG 0.456387 tig0024927:+71799 None:intergenic
GACCATCTGCTTCGAGCCTA+GGG 0.470227 tig0024927:+71800 None:intergenic
TCAACTCGCTCTCTGCGGAT+TGG 0.472898 tig0024927:-72555 Msa1421340:CDS
TTTGCCTGTGCCCAAATAGC+AGG 0.491915 tig0024927:-71567 Msa1421340:CDS
CTTTACCAATGAACAGAGGT+TGG 0.498726 tig0024927:+72153 None:intergenic
ATCATGGGTGGTCAAGTTGA+AGG 0.501757 tig0024927:+71512 None:intergenic
AGTTCAGGTGCTTTATAGCA+AGG 0.509440 tig0024927:+72247 None:intergenic
TCTATGGGATGCATATGCTT+AGG 0.515456 tig0024927:-72178 Msa1421340:CDS
GCAGTTGATATCTGGTCTAT+GGG 0.534989 tig0024927:-72193 Msa1421340:CDS
AAATCCTGCTATTTGGGCAC+AGG 0.541827 tig0024927:+71563 None:intergenic
TATAGAAGACACACCAGGCC+AGG 0.545839 tig0024927:+71969 None:intergenic
GCCTAGGGAATTCTGCTGCT+AGG 0.593322 tig0024927:+71815 None:intergenic
ATACCTTTGGCATGATTGGA+TGG 0.594825 tig0024927:+71923 None:intergenic
GCACGCCAACAGAAGAAACC+TGG 0.600125 tig0024927:-71987 Msa1421340:intron
TTCCCTAGGCTCGAAGCAGA+TGG 0.601568 tig0024927:-71802 Msa1421340:CDS
ACCTAGCAGCAGAATTCCCT+AGG 0.606461 tig0024927:-71816 Msa1421340:CDS
CTGCGTCAACTCGCTCTCTG+CGG 0.606752 tig0024927:-72560 Msa1421340:CDS
CCAACAGAAGAAACCTGGCC+TGG 0.607357 tig0024927:-71982 Msa1421340:intron
CGACCATCCAATCATGCCAA+AGG 0.634935 tig0024927:-71926 Msa1421340:intron
AATATTTCAGCCAATATCTG+CGG 0.646500 tig0024927:+72125 None:intergenic
TCATCTTTACCAATGAACAG+AGG 0.680053 tig0024927:+72149 None:intergenic
TTGCATATAGAAGACACACC+AGG 0.683383 tig0024927:+71964 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGTTTTATTTCAAAGA+AGG + tig0024927:71736-71755 None:intergenic 15.0%
!! ACAAATAGTATATATAGTTA+TGG + tig0024927:72000-72019 None:intergenic 15.0%
!! CACAAAATAATTAAATCAAT+TGG - tig0024927:72043-72062 Msa1421340:intron 15.0%
!!! TTTTAGTTTTATTTCAAAGA+AGG + tig0024927:71736-71755 None:intergenic 15.0%
!! ACAAATAGTATATATAGTTA+TGG + tig0024927:72000-72019 None:intergenic 15.0%
!! CACAAAATAATTAAATCAAT+TGG - tig0024927:72043-72062 Msa1421340:intron 15.0%
!! GAAATTATCTGACTTTAATT+TGG - tig0024927:71635-71654 Msa1421340:intron 20.0%
!! TCAAAGCTAATTAAGATATT+GGG + tig0024927:71765-71784 None:intergenic 20.0%
!! TTCAAAGCTAATTAAGATAT+TGG + tig0024927:71766-71785 None:intergenic 20.0%
!!! TTTATTTTCAACTTTCAGAT+GGG - tig0024927:71805-71824 Msa1421340:CDS 20.0%
!! CCTAAATTTATAAAAGGAAA+TGG + tig0024927:72212-72231 None:intergenic 20.0%
!!! CCATTTCCTTTTATAAATTT+AGG - tig0024927:72209-72228 Msa1421340:CDS 20.0%
!!! TAAACTATATTGGATTTTGT+AGG - tig0024927:72240-72259 Msa1421340:CDS 20.0%
!! ATCTACTGAATGTTTATTAA+TGG - tig0024927:72461-72480 Msa1421340:CDS 20.0%
!! GAAATTATCTGACTTTAATT+TGG - tig0024927:71635-71654 Msa1421340:intron 20.0%
!! TCAAAGCTAATTAAGATATT+GGG + tig0024927:71765-71784 None:intergenic 20.0%
!! TTCAAAGCTAATTAAGATAT+TGG + tig0024927:71766-71785 None:intergenic 20.0%
!!! TTTATTTTCAACTTTCAGAT+GGG - tig0024927:71805-71824 Msa1421340:CDS 20.0%
!! CCTAAATTTATAAAAGGAAA+TGG + tig0024927:72212-72231 None:intergenic 20.0%
!!! CCATTTCCTTTTATAAATTT+AGG - tig0024927:72209-72228 Msa1421340:CDS 20.0%
!!! TAAACTATATTGGATTTTGT+AGG - tig0024927:72240-72259 Msa1421340:CDS 20.0%
!! ATCTACTGAATGTTTATTAA+TGG - tig0024927:72461-72480 Msa1421340:CDS 20.0%
!! TCTGAAACCTAATAATATTC+TGG - tig0024927:71581-71600 Msa1421340:intron 25.0%
! TGTCATACATTAATAGAGTA+GGG + tig0024927:71692-71711 None:intergenic 25.0%
!!! GTTTATTTTCAACTTTCAGA+TGG - tig0024927:71804-71823 Msa1421340:CDS 25.0%
!!! AGATATTGGCTGAAATATTT+TGG - tig0024927:71958-71977 Msa1421340:CDS 25.0%
!!! TTTTATATGTAAAGCTTGCT+TGG - tig0024927:72070-72089 Msa1421340:intron 25.0%
! ATCCAATATAGTTTATTGTC+TGG + tig0024927:72235-72254 None:intergenic 25.0%
!! TCTGAAACCTAATAATATTC+TGG - tig0024927:71581-71600 Msa1421340:intron 25.0%
! TGTCATACATTAATAGAGTA+GGG + tig0024927:71692-71711 None:intergenic 25.0%
!!! GTTTATTTTCAACTTTCAGA+TGG - tig0024927:71804-71823 Msa1421340:CDS 25.0%
!!! AGATATTGGCTGAAATATTT+TGG - tig0024927:71958-71977 Msa1421340:CDS 25.0%
!!! TTTTATATGTAAAGCTTGCT+TGG - tig0024927:72070-72089 Msa1421340:intron 25.0%
! ATCCAATATAGTTTATTGTC+TGG + tig0024927:72235-72254 None:intergenic 25.0%
! TGTGTAGAATTTTGTGAGTA+TGG + tig0024927:71557-71576 None:intergenic 30.0%
ACTGAAGCCAGAATATTATT+AGG + tig0024927:71591-71610 None:intergenic 30.0%
!! TATCTGACTTTAATTTGGTG+CGG - tig0024927:71640-71659 Msa1421340:intron 30.0%
GTGTCATACATTAATAGAGT+AGG + tig0024927:71693-71712 None:intergenic 30.0%
AATATTTCAGCCAATATCTG+CGG + tig0024927:71957-71976 None:intergenic 30.0%
AAGTAGAACATGAATACCTT+TGG + tig0024927:72172-72191 None:intergenic 30.0%
GTCTGGCCTAAATTTATAAA+AGG + tig0024927:72218-72237 None:intergenic 30.0%
TCTTCTTTCAGTAAGTTAAC+TGG - tig0024927:72306-72325 Msa1421340:intron 30.0%
! TGTGTAGAATTTTGTGAGTA+TGG + tig0024927:71557-71576 None:intergenic 30.0%
ACTGAAGCCAGAATATTATT+AGG + tig0024927:71591-71610 None:intergenic 30.0%
!! TATCTGACTTTAATTTGGTG+CGG - tig0024927:71640-71659 Msa1421340:intron 30.0%
GTGTCATACATTAATAGAGT+AGG + tig0024927:71693-71712 None:intergenic 30.0%
AATATTTCAGCCAATATCTG+CGG + tig0024927:71957-71976 None:intergenic 30.0%
AAGTAGAACATGAATACCTT+TGG + tig0024927:72172-72191 None:intergenic 30.0%
GTCTGGCCTAAATTTATAAA+AGG + tig0024927:72218-72237 None:intergenic 30.0%
TCTTCTTTCAGTAAGTTAAC+TGG - tig0024927:72306-72325 Msa1421340:intron 30.0%
TTAATAGAGTAGGGTGAAAG+AGG + tig0024927:71683-71702 None:intergenic 35.0%
!! TAAAGCACCTGAACTTTTGA+TGG - tig0024927:71840-71859 Msa1421340:intron 35.0%
!! AAAGCACCTGAACTTTTGAT+GGG - tig0024927:71841-71860 Msa1421340:intron 35.0%
ATCAACTGCAGTTGAGTAAT+TGG + tig0024927:71875-71894 None:intergenic 35.0%
TCATCTTTACCAATGAACAG+AGG + tig0024927:71933-71952 None:intergenic 35.0%
TAAAGATGAACCGCAGATAT+TGG - tig0024927:71944-71963 Msa1421340:CDS 35.0%
ATGAATACCTTTGGCATGAT+TGG + tig0024927:72163-72182 None:intergenic 35.0%
GGCCAGACAATAAACTATAT+TGG - tig0024927:72230-72249 Msa1421340:CDS 35.0%
TTAATAGAGTAGGGTGAAAG+AGG + tig0024927:71683-71702 None:intergenic 35.0%
!! TAAAGCACCTGAACTTTTGA+TGG - tig0024927:71840-71859 Msa1421340:intron 35.0%
!! AAAGCACCTGAACTTTTGAT+GGG - tig0024927:71841-71860 Msa1421340:intron 35.0%
ATCAACTGCAGTTGAGTAAT+TGG + tig0024927:71875-71894 None:intergenic 35.0%
TCATCTTTACCAATGAACAG+AGG + tig0024927:71933-71952 None:intergenic 35.0%
TAAAGATGAACCGCAGATAT+TGG - tig0024927:71944-71963 Msa1421340:CDS 35.0%
ATGAATACCTTTGGCATGAT+TGG + tig0024927:72163-72182 None:intergenic 35.0%
GGCCAGACAATAAACTATAT+TGG - tig0024927:72230-72249 Msa1421340:CDS 35.0%
AGTTCAGGTGCTTTATAGCA+AGG + tig0024927:71835-71854 None:intergenic 40.0%
GAAGAACCCATCAAAAGTTC+AGG + tig0024927:71850-71869 None:intergenic 40.0%
! ACTCAACTGCAGTTGATATC+TGG - tig0024927:71878-71897 Msa1421340:intron 40.0%
! TGCAGTTGATATCTGGTCTA+TGG - tig0024927:71885-71904 Msa1421340:intron 40.0%
! GCAGTTGATATCTGGTCTAT+GGG - tig0024927:71886-71905 Msa1421340:intron 40.0%
TCTATGGGATGCATATGCTT+AGG - tig0024927:71901-71920 Msa1421340:intron 40.0%
CTTTACCAATGAACAGAGGT+TGG + tig0024927:71929-71948 None:intergenic 40.0%
TTGCATATAGAAGACACACC+AGG + tig0024927:72118-72137 None:intergenic 40.0%
ATACCTTTGGCATGATTGGA+TGG + tig0024927:72159-72178 None:intergenic 40.0%
! TAATGGCCAGTGTTTTGCAT+TGG - tig0024927:72478-72497 Msa1421340:CDS 40.0%
TTCTGTCCAATGCAAAACAC+TGG + tig0024927:72487-72506 None:intergenic 40.0%
! CAGCTGAAATCCTGCTATTT+GGG + tig0024927:72525-72544 None:intergenic 40.0%
TCAGCTGAAATCCTGCTATT+TGG + tig0024927:72526-72545 None:intergenic 40.0%
AGTTCAGGTGCTTTATAGCA+AGG + tig0024927:71835-71854 None:intergenic 40.0%
GAAGAACCCATCAAAAGTTC+AGG + tig0024927:71850-71869 None:intergenic 40.0%
! ACTCAACTGCAGTTGATATC+TGG - tig0024927:71878-71897 Msa1421340:intron 40.0%
! TGCAGTTGATATCTGGTCTA+TGG - tig0024927:71885-71904 Msa1421340:intron 40.0%
! GCAGTTGATATCTGGTCTAT+GGG - tig0024927:71886-71905 Msa1421340:intron 40.0%
TCTATGGGATGCATATGCTT+AGG - tig0024927:71901-71920 Msa1421340:intron 40.0%
CTTTACCAATGAACAGAGGT+TGG + tig0024927:71929-71948 None:intergenic 40.0%
TTGCATATAGAAGACACACC+AGG + tig0024927:72118-72137 None:intergenic 40.0%
ATACCTTTGGCATGATTGGA+TGG + tig0024927:72159-72178 None:intergenic 40.0%
! TAATGGCCAGTGTTTTGCAT+TGG - tig0024927:72478-72497 Msa1421340:CDS 40.0%
TTCTGTCCAATGCAAAACAC+TGG + tig0024927:72487-72506 None:intergenic 40.0%
! CAGCTGAAATCCTGCTATTT+GGG + tig0024927:72525-72544 None:intergenic 40.0%
TCAGCTGAAATCCTGCTATT+TGG + tig0024927:72526-72545 None:intergenic 40.0%
ATAGAGTAGGGTGAAAGAGG+AGG + tig0024927:71680-71699 None:intergenic 45.0%
! AAATCCTGCTATTTGGGCAC+AGG + tig0024927:72519-72538 None:intergenic 45.0%
ATAGAGTAGGGTGAAAGAGG+AGG + tig0024927:71680-71699 None:intergenic 45.0%
! AAATCCTGCTATTTGGGCAC+AGG + tig0024927:72519-72538 None:intergenic 45.0%
AGGCTCCAACCTCTGTTCAT+TGG - tig0024927:71921-71940 Msa1421340:intron 50.0%
TATAGAAGACACACCAGGCC+AGG + tig0024927:72113-72132 None:intergenic 50.0%
CGACCATCCAATCATGCCAA+AGG - tig0024927:72153-72172 Msa1421340:CDS 50.0%
ACCTAGCAGCAGAATTCCCT+AGG - tig0024927:72263-72282 Msa1421340:intron 50.0%
TTTGCCTGTGCCCAAATAGC+AGG - tig0024927:72512-72531 Msa1421340:CDS 50.0%
! AGAGCGAGTTGACGCAGAAA+TGG + tig0024927:71516-71535 None:intergenic 50.0%
AGGCTCCAACCTCTGTTCAT+TGG - tig0024927:71921-71940 Msa1421340:intron 50.0%
TATAGAAGACACACCAGGCC+AGG + tig0024927:72113-72132 None:intergenic 50.0%
CGACCATCCAATCATGCCAA+AGG - tig0024927:72153-72172 Msa1421340:CDS 50.0%
ACCTAGCAGCAGAATTCCCT+AGG - tig0024927:72263-72282 Msa1421340:intron 50.0%
TTTGCCTGTGCCCAAATAGC+AGG - tig0024927:72512-72531 Msa1421340:CDS 50.0%
TCAACTCGCTCTCTGCGGAT+TGG - tig0024927:71524-71543 Msa1421340:CDS 55.0%
GCACGCCAACAGAAGAAACC+TGG - tig0024927:72092-72111 Msa1421340:intron 55.0%
CCAGGCCAGGTTTCTTCTGT+TGG + tig0024927:72100-72119 None:intergenic 55.0%
CCAACAGAAGAAACCTGGCC+TGG - tig0024927:72097-72116 Msa1421340:intron 55.0%
GCCTAGGGAATTCTGCTGCT+AGG + tig0024927:72267-72286 None:intergenic 55.0%
TTCCCTAGGCTCGAAGCAGA+TGG - tig0024927:72277-72296 Msa1421340:intron 55.0%
GACCATCTGCTTCGAGCCTA+GGG + tig0024927:72282-72301 None:intergenic 55.0%
AGACCATCTGCTTCGAGCCT+AGG + tig0024927:72283-72302 None:intergenic 55.0%
TCAACTCGCTCTCTGCGGAT+TGG - tig0024927:71524-71543 Msa1421340:CDS 55.0%
GCACGCCAACAGAAGAAACC+TGG - tig0024927:72092-72111 Msa1421340:intron 55.0%
CCAGGCCAGGTTTCTTCTGT+TGG + tig0024927:72100-72119 None:intergenic 55.0%
CCAACAGAAGAAACCTGGCC+TGG - tig0024927:72097-72116 Msa1421340:intron 55.0%
GCCTAGGGAATTCTGCTGCT+AGG + tig0024927:72267-72286 None:intergenic 55.0%
TTCCCTAGGCTCGAAGCAGA+TGG - tig0024927:72277-72296 Msa1421340:intron 55.0%
GACCATCTGCTTCGAGCCTA+GGG + tig0024927:72282-72301 None:intergenic 55.0%
AGACCATCTGCTTCGAGCCT+AGG + tig0024927:72283-72302 None:intergenic 55.0%
CTGCGTCAACTCGCTCTCTG+CGG - tig0024927:71519-71538 Msa1421340:CDS 60.0%
CTGCGTCAACTCGCTCTCTG+CGG - tig0024927:71519-71538 Msa1421340:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0024927 gene 71516 72585 71516 ID=Msa1421340;Name=Msa1421340
tig0024927 mRNA 71516 72585 71516 ID=Msa1421340-mRNA-1;Parent=Msa1421340;Name=Msa1421340-mRNA-1;_AED=0.45;_eAED=0.65;_QI=0|0|0|0.6|1|0.6|5|0|168
tig0024927 exon 72445 72585 72445 ID=Msa1421340-mRNA-1:exon:2058;Parent=Msa1421340-mRNA-1
tig0024927 exon 72122 72278 72122 ID=Msa1421340-mRNA-1:exon:2057;Parent=Msa1421340-mRNA-1
tig0024927 exon 71927 71997 71927 ID=Msa1421340-mRNA-1:exon:2056;Parent=Msa1421340-mRNA-1
tig0024927 exon 71786 71839 71786 ID=Msa1421340-mRNA-1:exon:2055;Parent=Msa1421340-mRNA-1
tig0024927 exon 71516 71596 71516 ID=Msa1421340-mRNA-1:exon:2054;Parent=Msa1421340-mRNA-1
tig0024927 CDS 72445 72585 72445 ID=Msa1421340-mRNA-1:cds;Parent=Msa1421340-mRNA-1
tig0024927 CDS 72122 72278 72122 ID=Msa1421340-mRNA-1:cds;Parent=Msa1421340-mRNA-1
tig0024927 CDS 71927 71997 71927 ID=Msa1421340-mRNA-1:cds;Parent=Msa1421340-mRNA-1
tig0024927 CDS 71786 71839 71786 ID=Msa1421340-mRNA-1:cds;Parent=Msa1421340-mRNA-1
tig0024927 CDS 71516 71596 71516 ID=Msa1421340-mRNA-1:cds;Parent=Msa1421340-mRNA-1
Gene Sequence

>Msa1421340

TTTCTGCGTCAACTCGCTCTCTGCGGATTGGCTTACTGCCATACTCACAAAATTCTACACAGAGATCTGAAACCTAATAATATTCTGGCTTCAGTTGAAGAATTACTTGTCAATGAAGTGAAATTATCTGACTTTAATTTGATGGGTAATCCTTGCTATAAAGCACCTGAACTTTTGATGGGTTCTTCTACCAATTACTCAACTGCAGTTGATATCTGGTCTATGGGATGCATATGCTTAGGCTCCAACCTCTGTTCATTGGTAAAGATGAACCGCAGATATTGGCTGAAATATTTTGAAGAAACCTGGCCTGGTGTGTCTTCTATATGCAACTTTATTGAAGCACTCGACCATCCAATCATGCCAAAGGACCTAGCAGCAGAATTCCCTAGGCTCGAAGCAGATGGTCTTGATCTTCTTTCAAAAATGCTTTGCCTGTGCCCAAATAGCAGGATTTCAGCTGATCAAGCACTCGAGCAATCATACCTTCAACTTGACCACCCA
Protein sequence

>Msa1421340

FLRQLALCGLAYCHTHKILHRDLKPNNILASVEELLVNEVKLSDFNLMGNPCYKAPELLMGSSTNYSTAVDIWSMGCICLGSNLCSLVKMNRRYWLKYFEETWPGVSSICNFIEALDHPIMPKDLAAEFPRLEADGLDLLSKMLCLCPNSRISADQALEQSYLQLDHP