Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421480 | A0A072UZD5 | 80.925 | 173 | 9 | 3 | 1 | 149 | 1 | 173 | 4.76e-89 | 266 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1421480 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421480 | MtrunA17_Chr3g0120121 | 86.885 | 122 | 7 | 2 | 37 | 149 | 1 | 122 | 5.78e-69 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGCAAAATTTCCATTT+AGG | 0.111591 | tig0025053:-43822 | Msa1421480:CDS |
| GCAAAGCTAGATTTCAAAAT+TGG | 0.182403 | tig0025053:-43418 | Msa1421480:CDS |
| GAAGTGGAAATTACCCTAAA+TGG | 0.188466 | tig0025053:+43808 | None:intergenic |
| GAAAGCAAAATTTCCATTTA+GGG | 0.224342 | tig0025053:-43821 | Msa1421480:intron |
| TGCAGGCTAGAGCGATAATA+TGG | 0.385773 | tig0025053:-42851 | Msa1421480:intron |
| ATGGTTGGCAAGTCAAGAAA+TGG | 0.426643 | tig0025053:-42832 | Msa1421480:CDS |
| CAAGAAATGGAAGGAAAGAA+TGG | 0.466725 | tig0025053:-42819 | Msa1421480:CDS |
| ATGCACTAGGTTGCATTGAA+AGG | 0.491779 | tig0025053:+42766 | None:intergenic |
| TGCACTAGGTTGCATTGAAA+GGG | 0.509370 | tig0025053:+42767 | None:intergenic |
| ACACCACAGATTTGACGAAA+AGG | 0.545745 | tig0025053:-43198 | Msa1421480:CDS |
| TGCATTGAAAGGGATAGTGT+TGG | 0.550068 | tig0025053:+42777 | None:intergenic |
| GGCTAGAGCGATAATATGGT+TGG | 0.554001 | tig0025053:-42847 | Msa1421480:CDS |
| TCCTTGAGAAGAGAAAGAAA+AGG | 0.554506 | tig0025053:-42692 | Msa1421480:CDS |
| TTACAAATGCATGCCTCACA+TGG | 0.582863 | tig0025053:-42744 | Msa1421480:CDS |
| CTTGTTGCAAATCCTCGCAA+TGG | 0.611244 | tig0025053:-43221 | Msa1421480:CDS |
| AATCTGTGGTGTCCATTGCG+AGG | 0.627615 | tig0025053:+43209 | None:intergenic |
| GCATGCATTTGTAATGCACT+AGG | 0.632578 | tig0025053:+42753 | None:intergenic |
| AAGACATAATGCATTGAGTG+TGG | 0.644515 | tig0025053:-43853 | Msa1421480:CDS |
| TTGGCAAGTCAAGAAATGGA+AGG | 0.668042 | tig0025053:-42828 | Msa1421480:CDS |
| CTTTATGGAAAAGCCATGTG+AGG | 0.679093 | tig0025053:+42731 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAAAAGATAAGTATATTA+TGG | + | tig0025053:43603-43622 | None:intergenic | 15.0% |
| !!! | TTTTATCATTAGTTAAACTT+TGG | - | tig0025053:43645-43664 | Msa1421480:intron | 15.0% |
| !! | TGAAAAAGATAAGTATATTA+TGG | + | tig0025053:43603-43622 | None:intergenic | 15.0% |
| !!! | TTTTATCATTAGTTAAACTT+TGG | - | tig0025053:43645-43664 | Msa1421480:intron | 15.0% |
| !!! | TCTTGGAAATTTTTTTTTGT+AGG | - | tig0025053:43249-43268 | Msa1421480:CDS | 20.0% |
| !!! | TCTTGGAAATTTTTTTTTGT+AGG | - | tig0025053:43249-43268 | Msa1421480:CDS | 20.0% |
| ! | AGAAAGCAAAATTTCCATTT+AGG | - | tig0025053:42698-42717 | Msa1421480:CDS | 25.0% |
| ! | GAAAGCAAAATTTCCATTTA+GGG | - | tig0025053:42699-42718 | Msa1421480:CDS | 25.0% |
| ! | ATTTCAAGGGTGATATATAT+AGG | + | tig0025053:42931-42950 | None:intergenic | 25.0% |
| !!! | TATGCTTTTTGTTAAACTCT+TGG | - | tig0025053:43232-43251 | Msa1421480:CDS | 25.0% |
| !! | AAATTTTCTTCCACAAAACA+AGG | + | tig0025053:43401-43420 | None:intergenic | 25.0% |
| !!! | TTGTTTTGTGGAAGAAAATT+TGG | - | tig0025053:43400-43419 | Msa1421480:CDS | 25.0% |
| ! | AGAAAGCAAAATTTCCATTT+AGG | - | tig0025053:42698-42717 | Msa1421480:CDS | 25.0% |
| ! | GAAAGCAAAATTTCCATTTA+GGG | - | tig0025053:42699-42718 | Msa1421480:CDS | 25.0% |
| ! | ATTTCAAGGGTGATATATAT+AGG | + | tig0025053:42931-42950 | None:intergenic | 25.0% |
| !!! | TATGCTTTTTGTTAAACTCT+TGG | - | tig0025053:43232-43251 | Msa1421480:CDS | 25.0% |
| !! | AAATTTTCTTCCACAAAACA+AGG | + | tig0025053:43401-43420 | None:intergenic | 25.0% |
| !!! | TTGTTTTGTGGAAGAAAATT+TGG | - | tig0025053:43400-43419 | Msa1421480:CDS | 25.0% |
| ! | CTCAATGCATTATGTCTTTT+TGG | + | tig0025053:42665-42684 | None:intergenic | 30.0% |
| GCATGAGACATATATTTCAA+GGG | + | tig0025053:42944-42963 | None:intergenic | 30.0% | |
| TGCATGAGACATATATTTCA+AGG | + | tig0025053:42945-42964 | None:intergenic | 30.0% | |
| GTATGAGAAATGCTACATAT+TGG | + | tig0025053:43037-43056 | None:intergenic | 30.0% | |
| GCAAAGCTAGATTTCAAAAT+TGG | - | tig0025053:43102-43121 | Msa1421480:intron | 30.0% | |
| !!! | ATTTCTTCATCCTTGTTTTG+TGG | - | tig0025053:43388-43407 | Msa1421480:CDS | 30.0% |
| ATTATCGCATGCAAAAAGTT+AGG | + | tig0025053:43457-43476 | None:intergenic | 30.0% | |
| ATTAGTTAAACTTTGGTTGC+AGG | - | tig0025053:43652-43671 | Msa1421480:intron | 30.0% | |
| !!! | ATTTTGAAGTGATCGCTTTA+TGG | + | tig0025053:43807-43826 | None:intergenic | 30.0% |
| ! | CTCAATGCATTATGTCTTTT+TGG | + | tig0025053:42665-42684 | None:intergenic | 30.0% |
| GCATGAGACATATATTTCAA+GGG | + | tig0025053:42944-42963 | None:intergenic | 30.0% | |
| TGCATGAGACATATATTTCA+AGG | + | tig0025053:42945-42964 | None:intergenic | 30.0% | |
| GTATGAGAAATGCTACATAT+TGG | + | tig0025053:43037-43056 | None:intergenic | 30.0% | |
| GCAAAGCTAGATTTCAAAAT+TGG | - | tig0025053:43102-43121 | Msa1421480:intron | 30.0% | |
| !!! | ATTTCTTCATCCTTGTTTTG+TGG | - | tig0025053:43388-43407 | Msa1421480:CDS | 30.0% |
| ATTATCGCATGCAAAAAGTT+AGG | + | tig0025053:43457-43476 | None:intergenic | 30.0% | |
| ATTAGTTAAACTTTGGTTGC+AGG | - | tig0025053:43652-43671 | Msa1421480:intron | 30.0% | |
| !!! | ATTTTGAAGTGATCGCTTTA+TGG | + | tig0025053:43807-43826 | None:intergenic | 30.0% |
| AAGACATAATGCATTGAGTG+TGG | - | tig0025053:42667-42686 | Msa1421480:CDS | 35.0% | |
| GAAGTGGAAATTACCCTAAA+TGG | + | tig0025053:42715-42734 | None:intergenic | 35.0% | |
| AGGGTAATTTCCACTTCTAA+AGG | - | tig0025053:42718-42737 | Msa1421480:CDS | 35.0% | |
| GGGTAATTTCCACTTCTAAA+GGG | - | tig0025053:42719-42738 | Msa1421480:CDS | 35.0% | |
| TGTAGCTGCAAAGAAATAGA+AGG | - | tig0025053:42750-42769 | Msa1421480:CDS | 35.0% | |
| AATTGAATGACTAGACACGT+GGG | + | tig0025053:42894-42913 | None:intergenic | 35.0% | |
| AAATTGAATGACTAGACACG+TGG | + | tig0025053:42895-42914 | None:intergenic | 35.0% | |
| AGGGTGATATATATAGGTGT+CGG | + | tig0025053:42925-42944 | None:intergenic | 35.0% | |
| TGCTACATATTGGAGAACAA+AGG | + | tig0025053:43027-43046 | None:intergenic | 35.0% | |
| CGTGTATGGCAAAAAAAACA+TGG | - | tig0025053:43479-43498 | Msa1421480:intron | 35.0% | |
| ACATTGATCATTACACAGTG+TGG | + | tig0025053:43551-43570 | None:intergenic | 35.0% | |
| CAAGAAATGGAAGGAAAGAA+TGG | - | tig0025053:43701-43720 | Msa1421480:intron | 35.0% | |
| TCCTTGAGAAGAGAAAGAAA+AGG | - | tig0025053:43828-43847 | Msa1421480:CDS | 35.0% | |
| ! | TCCTTTTCTTTCTCTTCTCA+AGG | + | tig0025053:43832-43851 | None:intergenic | 35.0% |
| AAGACATAATGCATTGAGTG+TGG | - | tig0025053:42667-42686 | Msa1421480:CDS | 35.0% | |
| GAAGTGGAAATTACCCTAAA+TGG | + | tig0025053:42715-42734 | None:intergenic | 35.0% | |
| AGGGTAATTTCCACTTCTAA+AGG | - | tig0025053:42718-42737 | Msa1421480:CDS | 35.0% | |
| GGGTAATTTCCACTTCTAAA+GGG | - | tig0025053:42719-42738 | Msa1421480:CDS | 35.0% | |
| TGTAGCTGCAAAGAAATAGA+AGG | - | tig0025053:42750-42769 | Msa1421480:CDS | 35.0% | |
| AATTGAATGACTAGACACGT+GGG | + | tig0025053:42894-42913 | None:intergenic | 35.0% | |
| AAATTGAATGACTAGACACG+TGG | + | tig0025053:42895-42914 | None:intergenic | 35.0% | |
| AGGGTGATATATATAGGTGT+CGG | + | tig0025053:42925-42944 | None:intergenic | 35.0% | |
| TGCTACATATTGGAGAACAA+AGG | + | tig0025053:43027-43046 | None:intergenic | 35.0% | |
| CGTGTATGGCAAAAAAAACA+TGG | - | tig0025053:43479-43498 | Msa1421480:intron | 35.0% | |
| ACATTGATCATTACACAGTG+TGG | + | tig0025053:43551-43570 | None:intergenic | 35.0% | |
| CAAGAAATGGAAGGAAAGAA+TGG | - | tig0025053:43701-43720 | Msa1421480:intron | 35.0% | |
| TCCTTGAGAAGAGAAAGAAA+AGG | - | tig0025053:43828-43847 | Msa1421480:CDS | 35.0% | |
| ! | TCCTTTTCTTTCTCTTCTCA+AGG | + | tig0025053:43832-43851 | None:intergenic | 35.0% |
| ! | ATTTGCTTGCCCTTTAGAAG+TGG | + | tig0025053:42731-42750 | None:intergenic | 40.0% |
| ATACACATCGAGTTACTGTG+TGG | - | tig0025053:42782-42801 | Msa1421480:CDS | 40.0% | |
| ! | GGTGATATATATAGGTGTCG+GGG | + | tig0025053:42923-42942 | None:intergenic | 40.0% |
| GGGTGATATATATAGGTGTC+GGG | + | tig0025053:42924-42943 | None:intergenic | 40.0% | |
| ACACCACAGATTTGACGAAA+AGG | - | tig0025053:43322-43341 | Msa1421480:intron | 40.0% | |
| GCATGCGATAATTACGTGTA+TGG | - | tig0025053:43465-43484 | Msa1421480:intron | 40.0% | |
| TCATTACACAGTGTGGTCAA+GGG | + | tig0025053:43544-43563 | None:intergenic | 40.0% | |
| ATCATTACACAGTGTGGTCA+AGG | + | tig0025053:43545-43564 | None:intergenic | 40.0% | |
| ATGGTTGGCAAGTCAAGAAA+TGG | - | tig0025053:43688-43707 | Msa1421480:intron | 40.0% | |
| TTGGCAAGTCAAGAAATGGA+AGG | - | tig0025053:43692-43711 | Msa1421480:intron | 40.0% | |
| TGCATTGAAAGGGATAGTGT+TGG | + | tig0025053:43746-43765 | None:intergenic | 40.0% | |
| TGCACTAGGTTGCATTGAAA+GGG | + | tig0025053:43756-43775 | None:intergenic | 40.0% | |
| ATGCACTAGGTTGCATTGAA+AGG | + | tig0025053:43757-43776 | None:intergenic | 40.0% | |
| GCATGCATTTGTAATGCACT+AGG | + | tig0025053:43770-43789 | None:intergenic | 40.0% | |
| TTACAAATGCATGCCTCACA+TGG | - | tig0025053:43776-43795 | Msa1421480:intron | 40.0% | |
| CTTTATGGAAAAGCCATGTG+AGG | + | tig0025053:43792-43811 | None:intergenic | 40.0% | |
| ! | ATTTGCTTGCCCTTTAGAAG+TGG | + | tig0025053:42731-42750 | None:intergenic | 40.0% |
| ATACACATCGAGTTACTGTG+TGG | - | tig0025053:42782-42801 | Msa1421480:CDS | 40.0% | |
| ! | GGTGATATATATAGGTGTCG+GGG | + | tig0025053:42923-42942 | None:intergenic | 40.0% |
| GGGTGATATATATAGGTGTC+GGG | + | tig0025053:42924-42943 | None:intergenic | 40.0% | |
| ACACCACAGATTTGACGAAA+AGG | - | tig0025053:43322-43341 | Msa1421480:intron | 40.0% | |
| GCATGCGATAATTACGTGTA+TGG | - | tig0025053:43465-43484 | Msa1421480:intron | 40.0% | |
| TCATTACACAGTGTGGTCAA+GGG | + | tig0025053:43544-43563 | None:intergenic | 40.0% | |
| ATCATTACACAGTGTGGTCA+AGG | + | tig0025053:43545-43564 | None:intergenic | 40.0% | |
| ATGGTTGGCAAGTCAAGAAA+TGG | - | tig0025053:43688-43707 | Msa1421480:intron | 40.0% | |
| TTGGCAAGTCAAGAAATGGA+AGG | - | tig0025053:43692-43711 | Msa1421480:intron | 40.0% | |
| TGCATTGAAAGGGATAGTGT+TGG | + | tig0025053:43746-43765 | None:intergenic | 40.0% | |
| TGCACTAGGTTGCATTGAAA+GGG | + | tig0025053:43756-43775 | None:intergenic | 40.0% | |
| ATGCACTAGGTTGCATTGAA+AGG | + | tig0025053:43757-43776 | None:intergenic | 40.0% | |
| GCATGCATTTGTAATGCACT+AGG | + | tig0025053:43770-43789 | None:intergenic | 40.0% | |
| TTACAAATGCATGCCTCACA+TGG | - | tig0025053:43776-43795 | Msa1421480:intron | 40.0% | |
| CTTTATGGAAAAGCCATGTG+AGG | + | tig0025053:43792-43811 | None:intergenic | 40.0% | |
| CTTGTTGCAAATCCTCGCAA+TGG | - | tig0025053:43299-43318 | Msa1421480:intron | 45.0% | |
| ! | GCTCCTTTTCGTCAAATCTG+TGG | + | tig0025053:43328-43347 | None:intergenic | 45.0% |
| TGCAGGCTAGAGCGATAATA+TGG | - | tig0025053:43669-43688 | Msa1421480:intron | 45.0% | |
| GGCTAGAGCGATAATATGGT+TGG | - | tig0025053:43673-43692 | Msa1421480:intron | 45.0% | |
| CTTGTTGCAAATCCTCGCAA+TGG | - | tig0025053:43299-43318 | Msa1421480:intron | 45.0% | |
| ! | GCTCCTTTTCGTCAAATCTG+TGG | + | tig0025053:43328-43347 | None:intergenic | 45.0% |
| TGCAGGCTAGAGCGATAATA+TGG | - | tig0025053:43669-43688 | Msa1421480:intron | 45.0% | |
| GGCTAGAGCGATAATATGGT+TGG | - | tig0025053:43673-43692 | Msa1421480:intron | 45.0% | |
| AATCTGTGGTGTCCATTGCG+AGG | + | tig0025053:43314-43333 | None:intergenic | 50.0% | |
| AATCTGTGGTGTCCATTGCG+AGG | + | tig0025053:43314-43333 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0025053 | gene | 42653 | 43889 | 42653 | ID=Msa1421480;Name=Msa1421480 |
| tig0025053 | mRNA | 42653 | 43889 | 42653 | ID=Msa1421480-mRNA-1;Parent=Msa1421480;Name=Msa1421480-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|4|0|149 |
| tig0025053 | exon | 43822 | 43889 | 43822 | ID=Msa1421480-mRNA-1:exon:2153;Parent=Msa1421480-mRNA-1 |
| tig0025053 | exon | 43367 | 43450 | 43367 | ID=Msa1421480-mRNA-1:exon:2152;Parent=Msa1421480-mRNA-1 |
| tig0025053 | exon | 43190 | 43271 | 43190 | ID=Msa1421480-mRNA-1:exon:2151;Parent=Msa1421480-mRNA-1 |
| tig0025053 | exon | 42653 | 42868 | 42653 | ID=Msa1421480-mRNA-1:exon:2150;Parent=Msa1421480-mRNA-1 |
| tig0025053 | CDS | 43822 | 43889 | 43822 | ID=Msa1421480-mRNA-1:cds;Parent=Msa1421480-mRNA-1 |
| tig0025053 | CDS | 43367 | 43450 | 43367 | ID=Msa1421480-mRNA-1:cds;Parent=Msa1421480-mRNA-1 |
| tig0025053 | CDS | 43190 | 43271 | 43190 | ID=Msa1421480-mRNA-1:cds;Parent=Msa1421480-mRNA-1 |
| tig0025053 | CDS | 42653 | 42868 | 42653 | ID=Msa1421480-mRNA-1:cds;Parent=Msa1421480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
