AlfalfaGEDB | Msa1423750

M. sativa cultivar zhongmu-4 / Msa1423750

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF05142.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1423750	sp\|Q9XGX0\|SHI_ARATH	45.055	364	117	17	1	314	1	331	6.11e-72	228

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1423750	G7KGA2	84.848	330	27	8	1	314	1	323	3.63e-178	504

TFs/TRs:

Gene ID	Type	Classification
Msa1423750	TF	SRS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1423750	MtrunA17_Chr5g0404781	84.848	330	27	8	1	314	1	323	0.0	504
Msa1423750	MtrunA17_Chr8g0372461	52.023	346	119	12	1	314	1	331	4.14e-90	272
Msa1423750	MtrunA17_Chr3g0142171	42.500	320	141	12	1	309	1	288	3.65e-61	196
Msa1423750	MtrunA17_Chr1g0155791	51.739	230	86	6	99	314	120	338	3.82e-59	192
Msa1423750	MtrunA17_Chr4g0035591	46.853	143	56	1	101	243	20	142	1.29e-39	138
Msa1423750	MtrunA17_Chr5g0441921	48.366	153	67	2	101	241	124	276	2.28e-39	141
Msa1423750	MtrunA17_Chr3g0082511	44.375	160	73	3	101	246	134	291	7.62e-39	139
Msa1423750	MtrunA17_Chr8g0353111	36.957	230	105	8	43	249	78	290	1.43e-35	130
Msa1423750	MtrunA17_Chr2g0281741	62.162	37	14	0	99	135	148	184	1.85e-12	65.1
Msa1423750	MtrunA17_Chr0c01g0489181	59.459	37	15	0	99	135	65	101	6.34e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1423750	AT5G66350.1	45.055	364	117	17	1	314	1	331	6.21e-73	228
Msa1423750	AT3G51060.1	46.626	326	111	11	35	314	62	370	3.15e-72	228
Msa1423750	AT5G66350.3	45.014	351	112	16	1	301	1	320	2.06e-70	221
Msa1423750	AT5G66350.2	45.014	351	112	16	1	301	1	320	4.10e-70	221
Msa1423750	AT1G75520.1	39.788	377	133	13	1	314	1	346	1.60e-64	207
Msa1423750	AT1G19790.2	38.753	369	145	14	1	313	1	344	1.61e-63	204
Msa1423750	AT1G19790.1	38.753	369	145	14	1	313	1	344	1.61e-63	204
Msa1423750	AT4G36260.1	39.053	338	161	14	1	312	1	319	3.15e-50	169
Msa1423750	AT2G18120.1	41.600	250	90	9	1	243	6	206	3.97e-46	155
Msa1423750	AT2G21400.2	52.778	144	51	3	101	241	10	139	1.84e-44	150
Msa1423750	AT2G21400.4	52.778	144	51	3	101	241	9	138	2.01e-44	150
Msa1423750	AT2G18120.2	40.964	249	91	9	1	243	6	204	5.94e-44	150
Msa1423750	AT2G21400.3	51.773	141	42	3	101	241	10	124	7.39e-43	145
Msa1423750	AT2G21400.1	51.773	141	42	3	101	241	9	123	8.52e-43	145
Msa1423750	AT5G12330.1	44.937	158	70	2	101	241	112	269	9.81e-37	134
Msa1423750	AT5G12330.2	44.937	158	70	2	101	241	112	269	9.81e-37	134
Msa1423750	AT5G12330.4	44.937	158	70	2	101	241	199	356	1.95e-36	135
Msa1423750	AT3G54430.1	41.290	155	66	6	97	244	37	173	3.48e-29	110
Msa1423750	AT5G33210.2	61.364	88	24	2	99	177	15	101	4.14e-29	108
Msa1423750	AT5G33210.1	61.364	88	24	2	99	177	50	136	9.82e-29	109
Msa1423750	AT5G12330.3	86.957	46	6	0	101	146	112	157	5.65e-23	95.5

Find 82 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAAATTCACTTTGAAATA+TGG	0.160002	tig0025572:-30434	None:intergenic
GACAGAAGAGATAAGGTTTA+AGG	0.199519	tig0025572:-32025	None:intergenic
ATCATTCGCCTAAACAAATT+AGG	0.250682	tig0025572:+32165	Msa1423750:three_prime_UTR
CCCTGATGCTACTGCTGTTT+GGG	0.261168	tig0025572:+32047	Msa1423750:CDS
TACTCCTCTGGTGTTGTGTT+AGG	0.305453	tig0025572:+30775	Msa1423750:CDS
TGTTGTTGTTGAGATGATTT+AGG	0.309644	tig0025572:-31024	None:intergenic
TCCCTGATGCTACTGCTGTT+TGG	0.338077	tig0025572:+32046	Msa1423750:CDS
ATAATCCAACAACAGAATTC+TGG	0.358326	tig0025572:+30623	Msa1423750:CDS
CACTTATATTAATAACTACT+AGG	0.365900	tig0025572:-30354	None:intergenic
GAATTGAAGGTTTGGTTAGT+TGG	0.366672	tig0025572:-30326	None:intergenic
ACTAGAATTAGAGGCGTTTC+CGG	0.373528	tig0025572:-31974	None:intergenic
AATAGATCTACTGTGTTTGT+AGG	0.376062	tig0025572:+30832	Msa1423750:CDS
TGGGAATAATAGTTACAATA+TGG	0.377378	tig0025572:+31948	Msa1423750:CDS
AGAGTGAGTGAAGGGTTTGT+AGG	0.387801	tig0025572:-31099	None:intergenic
CATATTTCAAAGTGAATTTG+TGG	0.392116	tig0025572:+30435	Msa1423750:five_prime_UTR
TAACTATTATTCCCATGATC+TGG	0.405584	tig0025572:-31940	None:intergenic
TGAATTCTCCAGCAGAGTTC+AGG	0.409422	tig0025572:+31812	Msa1423750:CDS
CTACTAGGGTAAAGAATTGA+AGG	0.411713	tig0025572:-30339	None:intergenic
TATGATTATGGTCCAGATCA+TGG	0.413474	tig0025572:+31928	Msa1423750:CDS
CGAATGATGAATCAAGATCT+TGG	0.418725	tig0025572:-32150	None:intergenic
CTTTCTCTTCTTCTAGCAGC+AGG	0.421698	tig0025572:-30982	None:intergenic
AGGGTAAAGAATTGAAGGTT+TGG	0.424136	tig0025572:-30334	None:intergenic
AAACTCATGTTAAGAGTACT+TGG	0.425175	tig0025572:+30956	Msa1423750:CDS
ACAGAAGAGATAAGGTTTAA+GGG	0.430955	tig0025572:-32024	None:intergenic
GGTTTGGAATAGTAGTAGTA+TGG	0.436546	tig0025572:+30552	Msa1423750:exon
TCCCAAACAGCAGTAGCATC+AGG	0.439192	tig0025572:-32048	None:intergenic
TTATACCAGAATTCTGTTGT+TGG	0.445028	tig0025572:-30628	None:intergenic
TGTTAGGAGTGAGACCTACT+AGG	0.447826	tig0025572:+30791	Msa1423750:CDS
GAGTGTACCACTAGAATTAG+AGG	0.455284	tig0025572:-31983	None:intergenic
GCTGGAGAATTCACCACAGC+TGG	0.457156	tig0025572:-31802	None:intergenic
GTTGATGTTGATGAAGATGT+TGG	0.457884	tig0025572:-30705	None:intergenic
GATATAAGTTGTCAAGATTG+TGG	0.462555	tig0025572:+30862	Msa1423750:CDS
CCACAAGATCTTTACTCCTC+TGG	0.463212	tig0025572:+30763	Msa1423750:CDS
AAGAATTGCGTACCACTAGA+AGG	0.471301	tig0025572:-32120	None:intergenic
TCTAGTGGTACACTCCTTGA+AGG	0.471939	tig0025572:+31991	Msa1423750:CDS
TCTACTGTGTTTGTAGGAAG+TGG	0.474128	tig0025572:+30838	Msa1423750:CDS
TTGTTGGATTATTATTCTGT+TGG	0.477389	tig0025572:-30612	None:intergenic
CTCACGCACCTGAACTCTGC+TGG	0.492218	tig0025572:-31820	None:intergenic
TCAGAAGAACAAACCCTAGT+AGG	0.499085	tig0025572:-30805	None:intergenic
AGGGCTGAGCCACCCCTTCA+AGG	0.501243	tig0025572:-32005	None:intergenic
GAATGATGAATCAAGATCTT+GGG	0.507602	tig0025572:-32149	None:intergenic
CATCAGGGACAGAAGAGATA+AGG	0.508932	tig0025572:-32032	None:intergenic
ATATAAGTTGTCAAGATTGT+GGG	0.512750	tig0025572:+30863	Msa1423750:CDS
AAGGTTTGGTTAGTTGGTTG+AGG	0.513889	tig0025572:-30320	None:intergenic
TCACTCACTCTCCCTTCAGC+AGG	0.521572	tig0025572:+31111	Msa1423750:CDS
AGATCTTGTGGCTGCTGCTG+AGG	0.524505	tig0025572:-30751	None:intergenic
TAAACGAGTGCAAGCTAAAG+AGG	0.530926	tig0025572:-31076	None:intergenic
CTAGTGGTACACTCCTTGAA+GGG	0.532650	tig0025572:+31992	Msa1423750:CDS
AATAGTTACAATATGGCTAC+CGG	0.553111	tig0025572:+31955	Msa1423750:CDS
GGAGCTGGATACAAAGAAGA+AGG	0.554978	tig0025572:-32081	None:intergenic
GTTAGGAGTGAGACCTACTA+GGG	0.557590	tig0025572:+30792	Msa1423750:CDS
GAAGGCATGAAGGTATTAAG+TGG	0.559260	tig0025572:-32102	None:intergenic
TCAACAGCTGTAAACATTGG+AGG	0.560848	tig0025572:+31883	Msa1423750:CDS
CAAGTTCTACATCTCATATG+AGG	0.561087	tig0025572:-30907	None:intergenic
ATGATTATGGTCCAGATCAT+GGG	0.563960	tig0025572:+31929	Msa1423750:CDS
GTTGTTTCTAATTCATACCG+TGG	0.564425	tig0025572:+30667	Msa1423750:CDS
ATGAAGGTATTAAGTGGAGC+TGG	0.565074	tig0025572:-32096	None:intergenic
ATGAATCAAGATCTTGGGTG+GGG	0.569684	tig0025572:-32144	None:intergenic
ACTTATATTAATAACTACTA+GGG	0.573540	tig0025572:-30353	None:intergenic
TGGTACACTCCTTGAAGGGG+TGG	0.576962	tig0025572:+31996	Msa1423750:CDS
TGATGAATCAAGATCTTGGG+TGG	0.578391	tig0025572:-32146	None:intergenic
ATATAGTATATACCTGCTGA+AGG	0.578577	tig0025572:-31123	None:intergenic
AGAACTTGTTGCAAGAGTAG+AGG	0.587131	tig0025572:+30922	Msa1423750:CDS
TATTCAACAGCTGTAAACAT+TGG	0.588034	tig0025572:+31880	Msa1423750:CDS
CCAGAGGAGTAAAGATCTTG+TGG	0.588657	tig0025572:-30763	None:intergenic
CTGAAGGGAGAGTGAGTGAA+GGG	0.593490	tig0025572:-31107	None:intergenic
CAACAGCTGTAAACATTGGA+GGG	0.602052	tig0025572:+31884	Msa1423750:CDS
CTCAGTTGATGAAAACGAAG+AGG	0.603171	tig0025572:+31849	Msa1423750:CDS
AATACCTTCATGCCTTCTAG+TGG	0.605967	tig0025572:+32108	Msa1423750:CDS
GATGAATCAAGATCTTGGGT+GGG	0.609126	tig0025572:-32145	None:intergenic
CCCAAACAGCAGTAGCATCA+GGG	0.610175	tig0025572:-32047	None:intergenic
CGTACCACTAGAAGGCATGA+AGG	0.621664	tig0025572:-32112	None:intergenic
AACTCATGTTAAGAGTACTT+GGG	0.622414	tig0025572:+30957	Msa1423750:CDS
TGAATCAAGATCTTGGGTGG+GGG	0.629021	tig0025572:-32143	None:intergenic
AAATCATCTCAACAACAACA+TGG	0.641154	tig0025572:+31027	Msa1423750:CDS
TAGTGGTACACTCCTTGAAG+GGG	0.649565	tig0025572:+31993	Msa1423750:CDS
GCTGAAGGGAGAGTGAGTGA+AGG	0.652710	tig0025572:-31108	None:intergenic
TATAGTATATACCTGCTGAA+GGG	0.659960	tig0025572:-31122	None:intergenic
CACTCCTAACACAACACCAG+AGG	0.665208	tig0025572:-30779	None:intergenic
GTATACATATATATAGATCG+AGG	0.691769	tig0025572:+30500	Msa1423750:five_prime_UTR
GGAAACGCCTCTAATTCTAG+TGG	0.698556	tig0025572:+31976	Msa1423750:CDS
GGTTCCAAATCTCAAAACCA+CGG	0.709690	tig0025572:-30684	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTTATATTAATAACTACTA+GGG	-	tig0025572:30356-30375	None:intergenic	15.0%
!!	TTATTGTTTATAATAGTGAT+CGG	+	tig0025572:31608-31627	Msa1423750:intron	15.0%
!!	ACTTATATTAATAACTACTA+GGG	-	tig0025572:30356-30375	None:intergenic	15.0%
!!	TTATTGTTTATAATAGTGAT+CGG	+	tig0025572:31608-31627	Msa1423750:intron	15.0%
!!	CACTTATATTAATAACTACT+AGG	-	tig0025572:30357-30376	None:intergenic	20.0%
!!!	AATTTGTGGTTAATTTTGAT+TGG	+	tig0025572:30449-30468	Msa1423750:five_prime_UTR	20.0%
!!	AAACAAACAAACTATTAGTT+AGG	-	tig0025572:31720-31739	None:intergenic	20.0%
!!!	TGTTTTTTCCTAATTTGTTT+AGG	-	tig0025572:32176-32195	None:intergenic	20.0%
!!	CACTTATATTAATAACTACT+AGG	-	tig0025572:30357-30376	None:intergenic	20.0%
!!!	AATTTGTGGTTAATTTTGAT+TGG	+	tig0025572:30449-30468	Msa1423750:five_prime_UTR	20.0%
!!	AAACAAACAAACTATTAGTT+AGG	-	tig0025572:31720-31739	None:intergenic	20.0%
!!!	TGTTTTTTCCTAATTTGTTT+AGG	-	tig0025572:32176-32195	None:intergenic	20.0%
!!!	CACAAATTCACTTTGAAATA+TGG	-	tig0025572:30437-30456	None:intergenic	25.0%
!	CATATTTCAAAGTGAATTTG+TGG	+	tig0025572:30435-30454	Msa1423750:five_prime_UTR	25.0%
!	GTATACATATATATAGATCG+AGG	+	tig0025572:30500-30519	Msa1423750:five_prime_UTR	25.0%
!!!	ATGGCTAGTTTATTTTCATT+AGG	+	tig0025572:30571-30590	Msa1423750:CDS	25.0%
!	TTGTTGGATTATTATTCTGT+TGG	-	tig0025572:30615-30634	None:intergenic	25.0%
!	ATATAAGTTGTCAAGATTGT+GGG	+	tig0025572:30863-30882	Msa1423750:CDS	25.0%
!	GACATATATGAACAAAGAAA+AGG	-	tig0025572:31504-31523	None:intergenic	25.0%
!	GTTCTAACATTTACTTACTA+TGG	+	tig0025572:31551-31570	Msa1423750:intron	25.0%
!!	TATAACACAGCTTTATAGAA+AGG	-	tig0025572:31659-31678	None:intergenic	25.0%
!	AAAAAATTAAGGGCTATTAG+AGG	+	tig0025572:31768-31787	Msa1423750:intron	25.0%
!!	AGAGGAATTTTGTATGATTA+TGG	+	tig0025572:31916-31935	Msa1423750:CDS	25.0%
!	TGGGAATAATAGTTACAATA+TGG	+	tig0025572:31948-31967	Msa1423750:CDS	25.0%
!!!	CACAAATTCACTTTGAAATA+TGG	-	tig0025572:30437-30456	None:intergenic	25.0%
!	CATATTTCAAAGTGAATTTG+TGG	+	tig0025572:30435-30454	Msa1423750:five_prime_UTR	25.0%
!	GTATACATATATATAGATCG+AGG	+	tig0025572:30500-30519	Msa1423750:five_prime_UTR	25.0%
!!!	ATGGCTAGTTTATTTTCATT+AGG	+	tig0025572:30571-30590	Msa1423750:CDS	25.0%
!	TTGTTGGATTATTATTCTGT+TGG	-	tig0025572:30615-30634	None:intergenic	25.0%
!	ATATAAGTTGTCAAGATTGT+GGG	+	tig0025572:30863-30882	Msa1423750:CDS	25.0%
!	GACATATATGAACAAAGAAA+AGG	-	tig0025572:31504-31523	None:intergenic	25.0%
!	GTTCTAACATTTACTTACTA+TGG	+	tig0025572:31551-31570	Msa1423750:intron	25.0%
!!	TATAACACAGCTTTATAGAA+AGG	-	tig0025572:31659-31678	None:intergenic	25.0%
!	AAAAAATTAAGGGCTATTAG+AGG	+	tig0025572:31768-31787	Msa1423750:intron	25.0%
!!	AGAGGAATTTTGTATGATTA+TGG	+	tig0025572:31916-31935	Msa1423750:CDS	25.0%
!	TGGGAATAATAGTTACAATA+TGG	+	tig0025572:31948-31967	Msa1423750:CDS	25.0%
!!!	TCGAGGTTTTTTTCTTGATA+TGG	+	tig0025572:30517-30536	Msa1423750:five_prime_UTR	30.0%
!	TTGATATGGATTTTCGTTCA+GGG	+	tig0025572:30531-30550	Msa1423750:five_prime_UTR	30.0%
	ATAATCCAACAACAGAATTC+TGG	+	tig0025572:30623-30642	Msa1423750:CDS	30.0%
	TTATACCAGAATTCTGTTGT+TGG	-	tig0025572:30631-30650	None:intergenic	30.0%
	AATAGATCTACTGTGTTTGT+AGG	+	tig0025572:30832-30851	Msa1423750:CDS	30.0%
	GATATAAGTTGTCAAGATTG+TGG	+	tig0025572:30862-30881	Msa1423750:CDS	30.0%
!!	AAACTCATGTTAAGAGTACT+TGG	+	tig0025572:30956-30975	Msa1423750:CDS	30.0%
!!	AACTCATGTTAAGAGTACTT+GGG	+	tig0025572:30957-30976	Msa1423750:CDS	30.0%
!	TGTTGTTGTTGAGATGATTT+AGG	-	tig0025572:31027-31046	None:intergenic	30.0%
	AAATCATCTCAACAACAACA+TGG	+	tig0025572:31027-31046	Msa1423750:CDS	30.0%
	TATAGTATATACCTGCTGAA+GGG	-	tig0025572:31125-31144	None:intergenic	30.0%
	ATATAGTATATACCTGCTGA+AGG	-	tig0025572:31126-31145	None:intergenic	30.0%
!	CTTCTGAAAAAGATAAGTGA+AGG	+	tig0025572:31258-31277	Msa1423750:intron	30.0%
	ATTCTCTTCCAAAACTTTCT+CGG	-	tig0025572:31286-31305	None:intergenic	30.0%
!!!	ACACTGTTTTGGAAAAGAAA+AGG	-	tig0025572:31344-31363	None:intergenic	30.0%
	TTACTTACTATGGATACATG+AGG	+	tig0025572:31561-31580	Msa1423750:intron	30.0%
	TATTCAACAGCTGTAAACAT+TGG	+	tig0025572:31880-31899	Msa1423750:CDS	30.0%
	TAACTATTATTCCCATGATC+TGG	-	tig0025572:31943-31962	None:intergenic	30.0%
	AATAGTTACAATATGGCTAC+CGG	+	tig0025572:31955-31974	Msa1423750:CDS	30.0%
	ACAGAAGAGATAAGGTTTAA+GGG	-	tig0025572:32027-32046	None:intergenic	30.0%
	GAATGATGAATCAAGATCTT+GGG	-	tig0025572:32152-32171	None:intergenic	30.0%
	ATCATTCGCCTAAACAAATT+AGG	+	tig0025572:32165-32184	Msa1423750:three_prime_UTR	30.0%
!!!	TCGAGGTTTTTTTCTTGATA+TGG	+	tig0025572:30517-30536	Msa1423750:five_prime_UTR	30.0%
!	TTGATATGGATTTTCGTTCA+GGG	+	tig0025572:30531-30550	Msa1423750:five_prime_UTR	30.0%
	ATAATCCAACAACAGAATTC+TGG	+	tig0025572:30623-30642	Msa1423750:CDS	30.0%
	TTATACCAGAATTCTGTTGT+TGG	-	tig0025572:30631-30650	None:intergenic	30.0%
	AATAGATCTACTGTGTTTGT+AGG	+	tig0025572:30832-30851	Msa1423750:CDS	30.0%
	GATATAAGTTGTCAAGATTG+TGG	+	tig0025572:30862-30881	Msa1423750:CDS	30.0%
!!	AAACTCATGTTAAGAGTACT+TGG	+	tig0025572:30956-30975	Msa1423750:CDS	30.0%
!!	AACTCATGTTAAGAGTACTT+GGG	+	tig0025572:30957-30976	Msa1423750:CDS	30.0%
!	TGTTGTTGTTGAGATGATTT+AGG	-	tig0025572:31027-31046	None:intergenic	30.0%
	AAATCATCTCAACAACAACA+TGG	+	tig0025572:31027-31046	Msa1423750:CDS	30.0%
	TATAGTATATACCTGCTGAA+GGG	-	tig0025572:31125-31144	None:intergenic	30.0%
	ATATAGTATATACCTGCTGA+AGG	-	tig0025572:31126-31145	None:intergenic	30.0%
!	CTTCTGAAAAAGATAAGTGA+AGG	+	tig0025572:31258-31277	Msa1423750:intron	30.0%
	ATTCTCTTCCAAAACTTTCT+CGG	-	tig0025572:31286-31305	None:intergenic	30.0%
!!!	ACACTGTTTTGGAAAAGAAA+AGG	-	tig0025572:31344-31363	None:intergenic	30.0%
	TTACTTACTATGGATACATG+AGG	+	tig0025572:31561-31580	Msa1423750:intron	30.0%
	TATTCAACAGCTGTAAACAT+TGG	+	tig0025572:31880-31899	Msa1423750:CDS	30.0%
	TAACTATTATTCCCATGATC+TGG	-	tig0025572:31943-31962	None:intergenic	30.0%
	AATAGTTACAATATGGCTAC+CGG	+	tig0025572:31955-31974	Msa1423750:CDS	30.0%
	ACAGAAGAGATAAGGTTTAA+GGG	-	tig0025572:32027-32046	None:intergenic	30.0%
	GAATGATGAATCAAGATCTT+GGG	-	tig0025572:32152-32171	None:intergenic	30.0%
	ATCATTCGCCTAAACAAATT+AGG	+	tig0025572:32165-32184	Msa1423750:three_prime_UTR	30.0%
!	GAATTGAAGGTTTGGTTAGT+TGG	-	tig0025572:30329-30348	None:intergenic	35.0%
	AGGGTAAAGAATTGAAGGTT+TGG	-	tig0025572:30337-30356	None:intergenic	35.0%
	CTACTAGGGTAAAGAATTGA+AGG	-	tig0025572:30342-30361	None:intergenic	35.0%
!	CTTGATATGGATTTTCGTTC+AGG	+	tig0025572:30530-30549	Msa1423750:five_prime_UTR	35.0%
!	GGTTTGGAATAGTAGTAGTA+TGG	+	tig0025572:30552-30571	Msa1423750:exon	35.0%
!!	GTTGTTTCTAATTCATACCG+TGG	+	tig0025572:30667-30686	Msa1423750:CDS	35.0%
!	GTTGATGTTGATGAAGATGT+TGG	-	tig0025572:30708-30727	None:intergenic	35.0%
	CAAGTTCTACATCTCATATG+AGG	-	tig0025572:30910-30929	None:intergenic	35.0%
	TGTTGTTCTGACGAATGAAT+GGG	+	tig0025572:31361-31380	Msa1423750:intron	35.0%
	AATGAATGGGATCAATGACA+GGG	+	tig0025572:31374-31393	Msa1423750:intron	35.0%
!!	AAAGTAGTGTTGTTGGTTTG+TGG	-	tig0025572:31467-31486	None:intergenic	35.0%
	TGTGAAGAAAGTAGTGTTGT+TGG	-	tig0025572:31474-31493	None:intergenic	35.0%
	TATGATTATGGTCCAGATCA+TGG	+	tig0025572:31928-31947	Msa1423750:CDS	35.0%
	ATGATTATGGTCCAGATCAT+GGG	+	tig0025572:31929-31948	Msa1423750:CDS	35.0%
	GACAGAAGAGATAAGGTTTA+AGG	-	tig0025572:32028-32047	None:intergenic	35.0%
	CGAATGATGAATCAAGATCT+TGG	-	tig0025572:32153-32172	None:intergenic	35.0%
!	GAATTGAAGGTTTGGTTAGT+TGG	-	tig0025572:30329-30348	None:intergenic	35.0%
	AGGGTAAAGAATTGAAGGTT+TGG	-	tig0025572:30337-30356	None:intergenic	35.0%
	CTACTAGGGTAAAGAATTGA+AGG	-	tig0025572:30342-30361	None:intergenic	35.0%
!	CTTGATATGGATTTTCGTTC+AGG	+	tig0025572:30530-30549	Msa1423750:five_prime_UTR	35.0%
!	GGTTTGGAATAGTAGTAGTA+TGG	+	tig0025572:30552-30571	Msa1423750:exon	35.0%
!!	GTTGTTTCTAATTCATACCG+TGG	+	tig0025572:30667-30686	Msa1423750:CDS	35.0%
!	GTTGATGTTGATGAAGATGT+TGG	-	tig0025572:30708-30727	None:intergenic	35.0%
	CAAGTTCTACATCTCATATG+AGG	-	tig0025572:30910-30929	None:intergenic	35.0%
	TGTTGTTCTGACGAATGAAT+GGG	+	tig0025572:31361-31380	Msa1423750:intron	35.0%
	AATGAATGGGATCAATGACA+GGG	+	tig0025572:31374-31393	Msa1423750:intron	35.0%
!!	AAAGTAGTGTTGTTGGTTTG+TGG	-	tig0025572:31467-31486	None:intergenic	35.0%
	TGTGAAGAAAGTAGTGTTGT+TGG	-	tig0025572:31474-31493	None:intergenic	35.0%
	TATGATTATGGTCCAGATCA+TGG	+	tig0025572:31928-31947	Msa1423750:CDS	35.0%
	ATGATTATGGTCCAGATCAT+GGG	+	tig0025572:31929-31948	Msa1423750:CDS	35.0%
	GACAGAAGAGATAAGGTTTA+AGG	-	tig0025572:32028-32047	None:intergenic	35.0%
	CGAATGATGAATCAAGATCT+TGG	-	tig0025572:32153-32172	None:intergenic	35.0%
!	AAGGTTTGGTTAGTTGGTTG+AGG	-	tig0025572:30323-30342	None:intergenic	40.0%
!	ATGGATTTTCGTTCAGGGTT+TGG	+	tig0025572:30536-30555	Msa1423750:five_prime_UTR	40.0%
!!!	CATACCGTGGTTTTGAGATT+TGG	+	tig0025572:30680-30699	Msa1423750:CDS	40.0%
	GGTTCCAAATCTCAAAACCA+CGG	-	tig0025572:30687-30706	None:intergenic	40.0%
	TCAGAAGAACAAACCCTAGT+AGG	-	tig0025572:30808-30827	None:intergenic	40.0%
	TCTACTGTGTTTGTAGGAAG+TGG	+	tig0025572:30838-30857	Msa1423750:CDS	40.0%
!!	AGAACTTGTTGCAAGAGTAG+AGG	+	tig0025572:30922-30941	Msa1423750:CDS	40.0%
	TAAACGAGTGCAAGCTAAAG+AGG	-	tig0025572:31079-31098	None:intergenic	40.0%
!!!	TGAAGGTACCGAGAAAGTTT+TGG	+	tig0025572:31275-31294	Msa1423750:intron	40.0%
!	TCGTCAGAACAACACTGTTT+TGG	-	tig0025572:31355-31374	None:intergenic	40.0%
!	GTGTTGTTCTGACGAATGAA+TGG	+	tig0025572:31360-31379	Msa1423750:intron	40.0%
	GAATGAATGGGATCAATGAC+AGG	+	tig0025572:31373-31392	Msa1423750:intron	40.0%
!!	CTCAGTTGATGAAAACGAAG+AGG	+	tig0025572:31849-31868	Msa1423750:CDS	40.0%
	TCAACAGCTGTAAACATTGG+AGG	+	tig0025572:31883-31902	Msa1423750:CDS	40.0%
	CAACAGCTGTAAACATTGGA+GGG	+	tig0025572:31884-31903	Msa1423750:CDS	40.0%
!!!	ATTGGAGGGCATGTTTTTAG+AGG	+	tig0025572:31898-31917	Msa1423750:CDS	40.0%
!!	ACTAGAATTAGAGGCGTTTC+CGG	-	tig0025572:31977-31996	None:intergenic	40.0%
	GAGTGTACCACTAGAATTAG+AGG	-	tig0025572:31986-32005	None:intergenic	40.0%
!!	ATGAAGGTATTAAGTGGAGC+TGG	-	tig0025572:32099-32118	None:intergenic	40.0%
!!	GAAGGCATGAAGGTATTAAG+TGG	-	tig0025572:32105-32124	None:intergenic	40.0%
	AATACCTTCATGCCTTCTAG+TGG	+	tig0025572:32108-32127	Msa1423750:CDS	40.0%
	AAGAATTGCGTACCACTAGA+AGG	-	tig0025572:32123-32142	None:intergenic	40.0%
	ATGAATCAAGATCTTGGGTG+GGG	-	tig0025572:32147-32166	None:intergenic	40.0%
	GATGAATCAAGATCTTGGGT+GGG	-	tig0025572:32148-32167	None:intergenic	40.0%
	TGATGAATCAAGATCTTGGG+TGG	-	tig0025572:32149-32168	None:intergenic	40.0%
!	AAGGTTTGGTTAGTTGGTTG+AGG	-	tig0025572:30323-30342	None:intergenic	40.0%
!	ATGGATTTTCGTTCAGGGTT+TGG	+	tig0025572:30536-30555	Msa1423750:five_prime_UTR	40.0%
!!!	CATACCGTGGTTTTGAGATT+TGG	+	tig0025572:30680-30699	Msa1423750:CDS	40.0%
	GGTTCCAAATCTCAAAACCA+CGG	-	tig0025572:30687-30706	None:intergenic	40.0%
	TCAGAAGAACAAACCCTAGT+AGG	-	tig0025572:30808-30827	None:intergenic	40.0%
	TCTACTGTGTTTGTAGGAAG+TGG	+	tig0025572:30838-30857	Msa1423750:CDS	40.0%
!!	AGAACTTGTTGCAAGAGTAG+AGG	+	tig0025572:30922-30941	Msa1423750:CDS	40.0%
	TAAACGAGTGCAAGCTAAAG+AGG	-	tig0025572:31079-31098	None:intergenic	40.0%
!!!	TGAAGGTACCGAGAAAGTTT+TGG	+	tig0025572:31275-31294	Msa1423750:intron	40.0%
!	TCGTCAGAACAACACTGTTT+TGG	-	tig0025572:31355-31374	None:intergenic	40.0%
!	GTGTTGTTCTGACGAATGAA+TGG	+	tig0025572:31360-31379	Msa1423750:intron	40.0%
	GAATGAATGGGATCAATGAC+AGG	+	tig0025572:31373-31392	Msa1423750:intron	40.0%
!!	CTCAGTTGATGAAAACGAAG+AGG	+	tig0025572:31849-31868	Msa1423750:CDS	40.0%
	TCAACAGCTGTAAACATTGG+AGG	+	tig0025572:31883-31902	Msa1423750:CDS	40.0%
	CAACAGCTGTAAACATTGGA+GGG	+	tig0025572:31884-31903	Msa1423750:CDS	40.0%
!!!	ATTGGAGGGCATGTTTTTAG+AGG	+	tig0025572:31898-31917	Msa1423750:CDS	40.0%
!!	ACTAGAATTAGAGGCGTTTC+CGG	-	tig0025572:31977-31996	None:intergenic	40.0%
	GAGTGTACCACTAGAATTAG+AGG	-	tig0025572:31986-32005	None:intergenic	40.0%
!!	ATGAAGGTATTAAGTGGAGC+TGG	-	tig0025572:32099-32118	None:intergenic	40.0%
!!	GAAGGCATGAAGGTATTAAG+TGG	-	tig0025572:32105-32124	None:intergenic	40.0%
	AATACCTTCATGCCTTCTAG+TGG	+	tig0025572:32108-32127	Msa1423750:CDS	40.0%
	AAGAATTGCGTACCACTAGA+AGG	-	tig0025572:32123-32142	None:intergenic	40.0%
	ATGAATCAAGATCTTGGGTG+GGG	-	tig0025572:32147-32166	None:intergenic	40.0%
	GATGAATCAAGATCTTGGGT+GGG	-	tig0025572:32148-32167	None:intergenic	40.0%
	TGATGAATCAAGATCTTGGG+TGG	-	tig0025572:32149-32168	None:intergenic	40.0%
	CCAGAGGAGTAAAGATCTTG+TGG	-	tig0025572:30766-30785	None:intergenic	45.0%
	CCACAAGATCTTTACTCCTC+TGG	+	tig0025572:30763-30782	Msa1423750:CDS	45.0%
!!	TACTCCTCTGGTGTTGTGTT+AGG	+	tig0025572:30775-30794	Msa1423750:CDS	45.0%
	TGTTAGGAGTGAGACCTACT+AGG	+	tig0025572:30791-30810	Msa1423750:CDS	45.0%
	GTTAGGAGTGAGACCTACTA+GGG	+	tig0025572:30792-30811	Msa1423750:CDS	45.0%
	CTTTCTCTTCTTCTAGCAGC+AGG	-	tig0025572:30985-31004	None:intergenic	45.0%
	AGAGTGAGTGAAGGGTTTGT+AGG	-	tig0025572:31102-31121	None:intergenic	45.0%
!	TGTGGAAGAGTGAGTGTTGT+TGG	-	tig0025572:31449-31468	None:intergenic	45.0%
!	GGAGATGAATTTTCCAGCTG+TGG	+	tig0025572:31789-31808	Msa1423750:CDS	45.0%
	TGAATTCTCCAGCAGAGTTC+AGG	+	tig0025572:31812-31831	Msa1423750:CDS	45.0%
	GGAAACGCCTCTAATTCTAG+TGG	+	tig0025572:31976-31995	Msa1423750:CDS	45.0%
	TCTAGTGGTACACTCCTTGA+AGG	+	tig0025572:31991-32010	Msa1423750:CDS	45.0%
	CTAGTGGTACACTCCTTGAA+GGG	+	tig0025572:31992-32011	Msa1423750:CDS	45.0%
	TAGTGGTACACTCCTTGAAG+GGG	+	tig0025572:31993-32012	Msa1423750:CDS	45.0%
	CATCAGGGACAGAAGAGATA+AGG	-	tig0025572:32035-32054	None:intergenic	45.0%
	GGAGCTGGATACAAAGAAGA+AGG	-	tig0025572:32084-32103	None:intergenic	45.0%
	TGAATCAAGATCTTGGGTGG+GGG	-	tig0025572:32146-32165	None:intergenic	45.0%
	CACAGAACAGAACTGCAAAC+GGG	-	tig0025572:30225-30244	None:intergenic	45.0%
	GCACAGAACAGAACTGCAAA+CGG	-	tig0025572:30226-30245	None:intergenic	45.0%
	CCAGAGGAGTAAAGATCTTG+TGG	-	tig0025572:30766-30785	None:intergenic	45.0%
	CCACAAGATCTTTACTCCTC+TGG	+	tig0025572:30763-30782	Msa1423750:CDS	45.0%
!!	TACTCCTCTGGTGTTGTGTT+AGG	+	tig0025572:30775-30794	Msa1423750:CDS	45.0%
	TGTTAGGAGTGAGACCTACT+AGG	+	tig0025572:30791-30810	Msa1423750:CDS	45.0%
	GTTAGGAGTGAGACCTACTA+GGG	+	tig0025572:30792-30811	Msa1423750:CDS	45.0%
	CTTTCTCTTCTTCTAGCAGC+AGG	-	tig0025572:30985-31004	None:intergenic	45.0%
	AGAGTGAGTGAAGGGTTTGT+AGG	-	tig0025572:31102-31121	None:intergenic	45.0%
!	TGTGGAAGAGTGAGTGTTGT+TGG	-	tig0025572:31449-31468	None:intergenic	45.0%
!	GGAGATGAATTTTCCAGCTG+TGG	+	tig0025572:31789-31808	Msa1423750:CDS	45.0%
	TGAATTCTCCAGCAGAGTTC+AGG	+	tig0025572:31812-31831	Msa1423750:CDS	45.0%
	GGAAACGCCTCTAATTCTAG+TGG	+	tig0025572:31976-31995	Msa1423750:CDS	45.0%
	TCTAGTGGTACACTCCTTGA+AGG	+	tig0025572:31991-32010	Msa1423750:CDS	45.0%
	CTAGTGGTACACTCCTTGAA+GGG	+	tig0025572:31992-32011	Msa1423750:CDS	45.0%
	TAGTGGTACACTCCTTGAAG+GGG	+	tig0025572:31993-32012	Msa1423750:CDS	45.0%
	CATCAGGGACAGAAGAGATA+AGG	-	tig0025572:32035-32054	None:intergenic	45.0%
	GGAGCTGGATACAAAGAAGA+AGG	-	tig0025572:32084-32103	None:intergenic	45.0%
	TGAATCAAGATCTTGGGTGG+GGG	-	tig0025572:32146-32165	None:intergenic	45.0%
!!	AATTATCATTTAAAAAATTA+AGG	+	tig0025572:31757-31776	Msa1423750:intron	5.0%
!!	ATTATCATTTAAAAAATTAA+GGG	+	tig0025572:31758-31777	Msa1423750:intron	5.0%
!!	AATTATCATTTAAAAAATTA+AGG	+	tig0025572:31757-31776	Msa1423750:intron	5.0%
!!	ATTATCATTTAAAAAATTAA+GGG	+	tig0025572:31758-31777	Msa1423750:intron	5.0%
	CACTCCTAACACAACACCAG+AGG	-	tig0025572:30782-30801	None:intergenic	50.0%
	CTGAAGGGAGAGTGAGTGAA+GGG	-	tig0025572:31110-31129	None:intergenic	50.0%
	TCCCTGATGCTACTGCTGTT+TGG	+	tig0025572:32046-32065	Msa1423750:CDS	50.0%
	CCCAAACAGCAGTAGCATCA+GGG	-	tig0025572:32050-32069	None:intergenic	50.0%
!	CCCTGATGCTACTGCTGTTT+GGG	+	tig0025572:32047-32066	Msa1423750:CDS	50.0%
	TCCCAAACAGCAGTAGCATC+AGG	-	tig0025572:32051-32070	None:intergenic	50.0%
!	CGTACCACTAGAAGGCATGA+AGG	-	tig0025572:32115-32134	None:intergenic	50.0%
	CACTCCTAACACAACACCAG+AGG	-	tig0025572:30782-30801	None:intergenic	50.0%
	CTGAAGGGAGAGTGAGTGAA+GGG	-	tig0025572:31110-31129	None:intergenic	50.0%
	TCCCTGATGCTACTGCTGTT+TGG	+	tig0025572:32046-32065	Msa1423750:CDS	50.0%
	CCCAAACAGCAGTAGCATCA+GGG	-	tig0025572:32050-32069	None:intergenic	50.0%
!	CCCTGATGCTACTGCTGTTT+GGG	+	tig0025572:32047-32066	Msa1423750:CDS	50.0%
	TCCCAAACAGCAGTAGCATC+AGG	-	tig0025572:32051-32070	None:intergenic	50.0%
!	CGTACCACTAGAAGGCATGA+AGG	-	tig0025572:32115-32134	None:intergenic	50.0%
	AGATCTTGTGGCTGCTGCTG+AGG	-	tig0025572:30754-30773	None:intergenic	55.0%
	GCTGAAGGGAGAGTGAGTGA+AGG	-	tig0025572:31111-31130	None:intergenic	55.0%
	TCACTCACTCTCCCTTCAGC+AGG	+	tig0025572:31111-31130	Msa1423750:CDS	55.0%
	GCTGGAGAATTCACCACAGC+TGG	-	tig0025572:31805-31824	None:intergenic	55.0%
	TGGTACACTCCTTGAAGGGG+TGG	+	tig0025572:31996-32015	Msa1423750:CDS	55.0%
	AGATCTTGTGGCTGCTGCTG+AGG	-	tig0025572:30754-30773	None:intergenic	55.0%
	GCTGAAGGGAGAGTGAGTGA+AGG	-	tig0025572:31111-31130	None:intergenic	55.0%
	TCACTCACTCTCCCTTCAGC+AGG	+	tig0025572:31111-31130	Msa1423750:CDS	55.0%
	GCTGGAGAATTCACCACAGC+TGG	-	tig0025572:31805-31824	None:intergenic	55.0%
	TGGTACACTCCTTGAAGGGG+TGG	+	tig0025572:31996-32015	Msa1423750:CDS	55.0%
	CTCACGCACCTGAACTCTGC+TGG	-	tig0025572:31823-31842	None:intergenic	60.0%
	CTCACGCACCTGAACTCTGC+TGG	-	tig0025572:31823-31842	None:intergenic	60.0%
	AGGGCTGAGCCACCCCTTCA+AGG	-	tig0025572:32008-32027	None:intergenic	65.0%
	AGGGCTGAGCCACCCCTTCA+AGG	-	tig0025572:32008-32027	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0025572	gene	30225	32243	30225	ID=Msa1423750;Name=Msa1423750
tig0025572	mRNA	30225	32243	30225	ID=Msa1423750-mRNA-1;Parent=Msa1423750;Name=Msa1423750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=346\|1\|1\|1\|1\|1\|2\|82\|314
tig0025572	exon	30225	31132	30225	ID=Msa1423750-mRNA-1:exon:3392;Parent=Msa1423750-mRNA-1
tig0025572	exon	31779	32243	31779	ID=Msa1423750-mRNA-1:exon:3393;Parent=Msa1423750-mRNA-1
tig0025572	five_prime_UTR	30225	30570	30225	ID=Msa1423750-mRNA-1:five_prime_utr;Parent=Msa1423750-mRNA-1
tig0025572	CDS	30571	31132	30571	ID=Msa1423750-mRNA-1:cds;Parent=Msa1423750-mRNA-1
tig0025572	CDS	31779	32161	31779	ID=Msa1423750-mRNA-1:cds;Parent=Msa1423750-mRNA-1
tig0025572	three_prime_UTR	32162	32243	32162	ID=Msa1423750-mRNA-1:three_prime_utr;Parent=Msa1423750-mRNA-1

Gene Sequence

>Msa1423750

ATGGCTAGTTTATTTTCATTAGGAAGAACTGAAGAAGAAAACCAACAGAATAATAATCCAACAACAGAATTCTGGTATAATACGAATGATGATTCTGTTGTTTCTAATTCATACCGTGGTTTTGAGATTTGGAACCAACATCTTCATCAACATCAACAACACTTGCTTTCTTTACAACAACCTCAGCAGCAGCCACAAGATCTTTACTCCTCTGGTGTTGTGTTAGGAGTGAGACCTACTAGGGTTTGTTCTTCTGATGAGAATAGATCTACTGTGTTTGTAGGAAGTGGAGATATAAGTTGTCAAGATTGTGGGAATCAAGCTAAGAAAGATTGTCCTCATATGAGATGTAGAACTTGTTGCAAGAGTAGAGGTTTTGATTGTCAAACTCATGTTAAGAGTACTTGGGTTCCTGCTGCTAGAAGAAGAGAAAGACAACAACAACTTTCTGCTCCTAAATCATCTCAACAACAACATGGAGAGAGAGATCTTAACAATAACAATTCCTCTTTAGCTTGCACTCGTTTACCTACAAACCCTTCACTCACTCTCCCTTCAGCAGGGCTATTAGAGGAGATGAATTTTCCAGCTGTGGTGAATTCTCCAGCAGAGTTCAGGTGCGTGAGAGTTAGCTCAGTTGATGAAAACGAAGAGGAGTATGCATATTCAACAGCTGTAAACATTGGAGGGCATGTTTTTAGAGGAATTTTGTATGATTATGGTCCAGATCATGGGAATAATAGTTACAATATGGCTACCGGAAACGCCTCTAATTCTAGTGGTACACTCCTTGAAGGGGTGGCTCAGCCCTTAAACCTTATCTCTTCTGTCCCTGATGCTACTGCTGTTTGGGAAATTGTTGATCCTTCTTCTTTGTATCCAGCTCCACTTAATACCTTCATGCCTTCTAGTGGTACGCAATTCTTCCCCCACCCAAGATCTTGA

Protein sequence

>Msa1423750

MASLFSLGRTEEENQQNNNPTTEFWYNTNDDSVVSNSYRGFEIWNQHLHQHQQHLLSLQQPQQQPQDLYSSGVVLGVRPTRVCSSDENRSTVFVGSGDISCQDCGNQAKKDCPHMRCRTCCKSRGFDCQTHVKSTWVPAARRRERQQQLSAPKSSQQQHGERDLNNNNSSLACTRLPTNPSLTLPSAGLLEEMNFPAVVNSPAEFRCVRVSSVDENEEEYAYSTAVNIGGHVFRGILYDYGPDHGNNSYNMATGNASNSSGTLLEGVAQPLNLISSVPDATAVWEIVDPSSLYPAPLNTFMPSSGTQFFPHPRS*