AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1426250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1426250 MtrunA17_Chr8g0368721 86.842 266 30 1 132 392 47 312 8.71e-88 274
Msa1426250 MtrunA17_Chr8g0368721 74.860 179 36 3 1 176 1 173 3.74e-30 120
Msa1426250 MtrunA17_Chr2g0289181 44.206 233 69 2 398 569 1 233 8.32e-56 187
Msa1426250 MtrunA17_Chr6g0470151 46.130 323 136 7 133 438 56 357 3.88e-52 182
Msa1426250 MtrunA17_Chr6g0470151 41.791 268 116 8 1 238 1 258 8.98e-22 97.4
Msa1426250 MtrunA17_Chr8g0366921 49.658 292 127 4 120 392 30 320 1.13e-45 163
Msa1426250 MtrunA17_Chr8g0366921 47.584 269 96 6 1 239 1 254 9.42e-35 133
Msa1426250 MtrunA17_Chr1g0202461 53.535 99 44 2 388 486 239 335 1.93e-28 117
Msa1426250 MtrunA17_Chr7g0266621 44.569 267 132 4 131 392 47 302 1.85e-26 109
Msa1426250 MtrunA17_Chr7g0266621 72.289 83 23 0 1 83 1 83 3.19e-21 94.4
Msa1426250 MtrunA17_Chr2g0293901 56.198 121 49 2 135 255 54 170 2.74e-26 106
Msa1426250 MtrunA17_Chr2g0293901 46.927 179 80 6 1 173 1 170 5.18e-22 94.4
Msa1426250 MtrunA17_Chr8g0348311 46.939 98 49 2 388 485 134 228 8.87e-24 102
Msa1426250 MtrunA17_Chr7g0248691 47.917 96 49 1 390 485 133 227 1.59e-23 101
Msa1426250 MtrunA17_Chr2g0293481 45.133 113 50 3 391 493 238 348 2.01e-21 97.8
Msa1426250 MtrunA17_Chr8g0387501 56.944 72 30 1 412 483 130 200 7.17e-21 92.8
Msa1426250 MtrunA17_Chr4g0053281 51.471 68 33 0 412 479 267 334 3.92e-19 89.0
Msa1426250 MtrunA17_Chr3g0139021 43.750 96 53 1 382 476 284 379 1.24e-18 88.2
Msa1426250 MtrunA17_Chr5g0401821 51.471 68 33 0 412 479 200 267 1.39e-18 86.3
Msa1426250 MtrunA17_Chr4g0071521 56.716 67 28 1 407 473 242 307 1.86e-18 86.7
Msa1426250 MtrunA17_Chr2g0319961 56.061 66 29 0 406 471 75 140 3.52e-18 84.7
Msa1426250 MtrunA17_Chr8g0379461 53.968 63 29 0 409 471 74 136 4.45e-18 84.0
Msa1426250 MtrunA17_Chr1g0184691 57.627 59 25 0 412 470 294 352 1.72e-17 84.3
Msa1426250 MtrunA17_Chr7g0270051 55.000 60 27 0 412 471 54 113 2.91e-17 82.0
Msa1426250 MtrunA17_Chr1g0147881 47.826 69 36 0 412 480 263 331 3.46e-17 83.2
Msa1426250 MtrunA17_Chr2g0284321 53.968 63 29 0 409 471 33 95 5.10e-17 80.1
Msa1426250 MtrunA17_Chr7g0228941 54.098 61 28 0 412 472 53 113 8.51e-17 77.0
Msa1426250 MtrunA17_Chr7g0263531 50.000 76 36 2 407 482 185 258 2.29e-15 78.6
Msa1426250 MtrunA17_Chr7g0263531 41.584 101 44 4 388 474 324 423 1.77e-14 75.9
Msa1426250 MtrunA17_Chr7g0243891 51.220 82 35 3 394 475 202 278 5.65e-15 77.8
Msa1426250 MtrunA17_Chr7g0243891 39.189 74 44 1 411 484 374 446 2.85e-11 66.2
Msa1426250 MtrunA17_Chr7g0218081 47.826 69 34 2 407 475 224 290 6.24e-15 77.8
Msa1426250 MtrunA17_Chr7g0218081 47.761 67 34 1 411 477 410 475 6.47e-15 77.8
Msa1426250 MtrunA17_Chr7g0266611 57.576 66 28 0 466 531 2 67 8.58e-15 72.4
Msa1426250 MtrunA17_Chr3g0079691 49.315 73 35 2 409 481 278 348 9.03e-15 77.4
Msa1426250 MtrunA17_Chr3g0079691 43.478 69 38 1 411 479 495 562 4.48e-13 72.0
Msa1426250 MtrunA17_Chr7g0249311 44.304 79 42 2 401 479 169 245 4.44e-14 75.5
Msa1426250 MtrunA17_Chr7g0249311 38.095 105 50 3 411 515 392 481 1.22e-13 73.9
Msa1426250 MtrunA17_Chr5g0418191 46.269 67 35 1 411 477 320 385 8.05e-14 73.9
Msa1426250 MtrunA17_Chr5g0418191 35.971 139 75 6 407 542 147 274 1.10e-13 73.2
Msa1426250 MtrunA17_Chr1g0194481 44.286 70 38 1 411 480 158 226 9.30e-14 72.8
Msa1426250 MtrunA17_Chr4g0042831 43.750 80 44 1 411 490 278 356 9.73e-14 73.2
Msa1426250 MtrunA17_Chr8g0387981 41.379 87 45 2 386 466 103 189 1.03e-13 72.8
Msa1426250 MtrunA17_Chr4g0004911 52.174 69 31 2 404 471 176 243 2.05e-13 72.8
Msa1426250 MtrunA17_Chr4g0004911 44.262 61 33 1 411 471 336 395 6.08e-12 68.2
Msa1426250 MtrunA17_Chr4g0074001 36.111 108 56 3 376 471 312 418 2.19e-13 72.8
Msa1426250 MtrunA17_Chr7g0270191 46.377 69 36 1 411 479 145 212 2.66e-13 70.9
Msa1426250 MtrunA17_Chr6g0470161 42.268 97 49 2 480 569 24 120 3.14e-13 66.6
Msa1426250 MtrunA17_Chr4g0051881 49.180 61 30 1 411 471 178 237 3.41e-13 71.2
Msa1426250 MtrunA17_Chr5g0443131 50.000 62 30 1 411 472 157 217 3.45e-13 70.9
Msa1426250 MtrunA17_Chr3g0106491 50.000 66 31 2 410 475 197 260 5.89e-13 71.2
Msa1426250 MtrunA17_Chr3g0101131 48.529 68 33 2 404 471 236 301 1.33e-12 70.5
Msa1426250 MtrunA17_Chr3g0101131 41.791 67 38 1 411 477 415 480 1.72e-12 70.1
Msa1426250 MtrunA17_Chr4g0068541 38.571 70 42 1 411 480 208 276 1.58e-12 69.7
Msa1426250 MtrunA17_Chr5g0432161 53.226 62 27 2 410 471 243 302 1.90e-12 70.1
Msa1426250 MtrunA17_Chr5g0432161 44.444 63 34 1 411 473 421 482 6.07e-12 68.6
Msa1426250 MtrunA17_Chr5g0445311 41.791 67 38 1 411 477 364 429 1.95e-12 69.7
Msa1426250 MtrunA17_Chr5g0445311 48.438 64 31 2 408 471 197 258 4.44e-12 68.6
Msa1426250 MtrunA17_Chr3g0129321 47.619 63 30 3 411 472 118 178 2.44e-12 66.2
Msa1426250 MtrunA17_Chr2g0294231 44.615 65 35 1 411 475 203 266 2.57e-12 68.9
Msa1426250 MtrunA17_Chr7g0249301 46.032 63 33 1 411 473 292 353 4.16e-12 68.2
Msa1426250 MtrunA17_Chr7g0249301 47.541 61 30 2 411 471 62 120 1.33e-11 66.6
Msa1426250 MtrunA17_Chr7g0238921 48.387 62 25 2 410 468 123 180 6.80e-12 67.0
Msa1426250 MtrunA17_Chr2g0302391 41.667 60 35 0 412 471 212 271 7.18e-12 67.8
Msa1426250 MtrunA17_Chr3g0126391 37.209 86 45 3 389 466 106 190 7.77e-12 67.0
Msa1426250 MtrunA17_Chr3g0136961 41.772 79 43 1 411 489 138 213 8.15e-12 66.2
Msa1426250 MtrunA17_Chr8g0383251 38.889 90 49 4 386 471 80 167 8.25e-12 64.3
Msa1426250 MtrunA17_Chr3g0099641 33.708 89 47 1 383 471 235 311 1.39e-11 67.0
Msa1426250 MtrunA17_Chr4g0001361 41.026 78 45 1 411 487 162 239 3.19e-11 64.7
Msa1426250 MtrunA17_Chr5g0418781 40.000 85 49 2 384 468 98 180 3.49e-11 64.3
Msa1426250 MtrunA17_Chr1g0188941 42.623 61 34 1 411 471 51 110 3.68e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1426250 AT5G52830.1 53.535 99 44 2 388 486 143 239 8.92e-28 115
Msa1426250 AT4G01250.1 37.584 149 82 5 339 485 62 201 2.77e-25 106
Msa1426250 AT4G23550.1 46.809 94 49 1 395 488 124 216 1.36e-21 96.3
Msa1426250 AT5G45050.4 51.613 93 43 2 388 480 1019 1109 9.22e-21 97.4
Msa1426250 AT5G45050.1 51.613 93 43 2 388 480 1158 1248 1.15e-20 97.4
Msa1426250 AT5G45050.2 51.613 93 43 2 388 480 1130 1220 1.26e-20 97.1
Msa1426250 AT1G30650.1 56.522 69 29 1 412 480 218 285 1.86e-19 91.7
Msa1426250 AT2G34830.1 56.522 69 29 1 412 480 216 283 1.87e-19 91.7
Msa1426250 AT2G34830.2 56.522 69 29 1 412 480 225 292 2.02e-19 91.7
Msa1426250 AT4G31550.2 35.766 137 75 2 353 476 174 310 3.71e-19 89.4
Msa1426250 AT4G31550.1 52.308 65 31 0 412 476 247 311 5.02e-19 89.0
Msa1426250 AT2G23320.1 56.140 57 25 0 412 468 241 297 3.56e-18 86.3
Msa1426250 AT2G24570.1 49.231 65 33 0 412 476 244 308 7.40e-18 85.5
Msa1426250 AT4G24240.1 48.810 84 38 2 389 468 256 338 1.85e-17 84.7
Msa1426250 AT5G28650.1 51.515 66 32 0 412 477 263 328 1.89e-17 84.3
Msa1426250 AT1G29280.1 42.424 99 53 1 377 471 36 134 2.45e-17 82.4
Msa1426250 AT3G04670.1 51.515 66 32 0 412 477 263 328 2.78e-17 83.6
Msa1426250 AT3G58710.1 51.471 68 33 0 409 476 69 136 4.67e-17 82.0
Msa1426250 AT3G58710.2 51.471 68 33 0 409 476 68 135 4.86e-17 82.0
Msa1426250 AT3G01080.3 46.154 78 37 3 407 484 201 273 1.09e-16 82.0
Msa1426250 AT3G01080.2 46.154 78 37 3 407 484 201 273 4.33e-16 81.3
Msa1426250 AT3G01080.2 47.541 61 31 1 411 471 344 403 1.47e-13 73.6
Msa1426250 AT3G01080.1 46.154 78 37 3 407 484 163 235 5.14e-16 80.9
Msa1426250 AT3G01080.1 47.541 61 31 1 411 471 306 365 1.98e-13 72.8
Msa1426250 AT2G30590.1 54.237 59 27 0 412 470 314 372 1.49e-15 79.3
Msa1426250 AT2G38470.1 50.000 80 36 3 392 471 167 242 6.52e-15 78.2
Msa1426250 AT2G38470.1 42.466 73 40 2 411 483 362 432 3.67e-12 69.3
Msa1426250 AT2G47260.1 37.391 115 66 3 384 494 148 260 1.63e-14 75.5
Msa1426250 AT2G03340.1 47.761 67 34 1 411 477 415 480 3.02e-14 75.9
Msa1426250 AT2G03340.1 47.143 70 35 2 407 476 246 313 3.19e-13 72.8
Msa1426250 AT3G62340.1 40.476 84 48 2 411 494 118 199 4.50e-14 73.2
Msa1426250 AT5G56270.1 34.194 155 78 5 409 541 271 423 6.98e-14 75.1
Msa1426250 AT5G56270.1 44.444 63 34 1 411 473 487 548 3.57e-12 69.7
Msa1426250 AT4G26440.1 45.946 74 38 2 409 482 176 247 1.04e-13 74.3
Msa1426250 AT4G26440.1 41.667 72 41 1 411 482 372 442 2.59e-11 66.6
Msa1426250 AT2G21900.1 39.362 94 52 2 384 473 78 170 1.36e-13 70.5
Msa1426250 AT4G30935.1 35.780 109 57 3 375 471 283 390 1.52e-13 73.6
Msa1426250 AT1G13960.1 39.796 98 49 4 392 484 205 297 2.23e-13 73.2
Msa1426250 AT1G13960.1 47.541 61 31 1 411 471 409 468 6.86e-13 71.6
Msa1426250 AT1G64000.1 38.095 84 48 3 386 468 90 170 5.30e-13 68.6
Msa1426250 AT1G13960.2 46.154 78 37 3 407 484 72 144 6.13e-13 70.9
Msa1426250 AT1G13960.2 47.541 61 31 1 411 471 256 315 1.61e-12 69.7
Msa1426250 AT2G30250.1 50.000 60 29 1 412 471 329 387 6.40e-13 71.2
Msa1426250 AT2G30250.1 48.387 62 30 2 410 471 165 224 1.76e-11 66.6
Msa1426250 AT5G43290.1 55.172 58 25 1 411 468 114 170 8.16e-13 69.3
Msa1426250 AT5G07100.1 35.455 110 64 3 410 513 116 224 8.63e-13 70.1
Msa1426250 AT5G07100.1 40.580 69 40 1 411 479 234 301 8.13e-12 67.0
Msa1426250 AT5G41570.1 40.000 85 48 3 386 468 71 154 1.16e-12 67.0
Msa1426250 AT2G37260.1 38.095 84 48 2 388 468 323 405 1.78e-12 70.1
Msa1426250 AT2G37260.1 50.000 66 31 2 407 472 161 224 8.52e-12 67.8
Msa1426250 AT2G40740.1 36.957 92 53 2 380 466 139 230 2.44e-12 68.2
Msa1426250 AT1G55600.1 46.970 66 33 2 406 471 303 366 2.47e-12 69.7
Msa1426250 AT2G37260.3 38.095 84 48 2 388 468 243 325 3.37e-12 68.6
Msa1426250 AT2G37260.3 49.254 67 32 2 407 473 81 145 1.84e-11 66.2
Msa1426250 AT2G37260.2 38.095 84 48 2 388 468 241 323 3.45e-12 68.6
Msa1426250 AT2G37260.2 49.254 67 32 2 407 473 79 143 1.90e-11 66.2
Msa1426250 AT2G40740.3 36.957 92 53 2 380 466 148 239 3.49e-12 68.2
Msa1426250 AT4G26640.7 38.462 104 57 5 410 510 170 269 3.82e-12 69.3
Msa1426250 AT4G26640.7 40.984 61 35 1 411 471 341 400 5.85e-11 65.5
Msa1426250 AT1G80840.1 43.548 62 35 0 411 472 146 207 3.94e-12 67.8
Msa1426250 AT4G26640.2 38.462 104 57 5 410 510 210 309 4.49e-12 69.3
Msa1426250 AT4G26640.2 40.984 61 35 1 411 471 381 440 6.31e-11 65.5
Msa1426250 AT1G29860.1 37.079 89 47 2 411 499 136 215 4.53e-12 67.4
Msa1426250 AT4G18170.1 41.026 78 44 2 391 468 153 228 5.89e-12 67.4
Msa1426250 AT4G26640.1 38.462 104 57 5 410 510 138 237 6.16e-12 68.6
Msa1426250 AT4G26640.1 40.984 61 35 1 411 471 309 368 7.98e-11 65.1
Msa1426250 AT2G04880.1 48.333 60 30 1 412 471 308 366 6.19e-12 68.6
Msa1426250 AT2G04880.2 48.333 60 30 1 412 471 284 342 6.63e-12 68.6
Msa1426250 AT5G07100.2 35.455 110 64 3 410 513 23 131 6.70e-12 65.9
Msa1426250 AT5G07100.2 44.262 61 33 1 411 471 141 200 3.46e-11 63.5
Msa1426250 AT5G07100.4 35.455 110 64 3 410 513 23 131 6.70e-12 65.9
Msa1426250 AT5G07100.4 44.262 61 33 1 411 471 141 200 3.46e-11 63.5
Msa1426250 AT5G07100.5 35.455 110 64 3 410 513 23 131 6.70e-12 65.9
Msa1426250 AT5G07100.5 44.262 61 33 1 411 471 141 200 3.46e-11 63.5
Msa1426250 AT5G07100.3 35.455 110 64 3 410 513 23 131 6.70e-12 65.9
Msa1426250 AT5G07100.3 44.262 61 33 1 411 471 141 200 3.46e-11 63.5
Msa1426250 AT1G69310.4 39.535 86 50 2 384 469 121 204 7.45e-12 66.6
Msa1426250 AT1G69310.2 39.535 86 50 2 384 469 121 204 7.45e-12 66.6
Msa1426250 AT1G69310.1 39.535 86 50 2 384 469 121 204 7.45e-12 66.6
Msa1426250 AT2G44745.1 37.374 99 58 4 372 468 105 201 7.61e-12 65.5
Msa1426250 AT4G26640.5 39.252 107 52 6 410 510 34 133 9.27e-12 67.4
Msa1426250 AT4G26640.5 40.984 61 35 1 411 471 205 264 8.86e-11 64.3
Msa1426250 AT4G26640.6 39.252 107 52 6 410 510 34 133 9.27e-12 67.4
Msa1426250 AT4G26640.6 40.984 61 35 1 411 471 205 264 8.86e-11 64.3
Msa1426250 AT4G26640.4 39.252 107 52 6 410 510 34 133 9.27e-12 67.4
Msa1426250 AT4G26640.4 40.984 61 35 1 411 471 205 264 8.86e-11 64.3
Msa1426250 AT4G26640.3 39.252 107 52 6 410 510 34 133 9.27e-12 67.4
Msa1426250 AT4G26640.3 40.984 61 35 1 411 471 205 264 8.86e-11 64.3
Msa1426250 AT5G49520.1 44.828 58 31 1 411 468 221 277 1.19e-11 67.4
Msa1426250 AT2G46400.1 43.182 88 45 3 384 466 74 161 2.24e-11 65.5
Msa1426250 AT5G45260.1 41.026 78 43 2 412 487 1209 1285 2.37e-11 67.4
Msa1426250 AT5G46350.1 46.552 58 30 1 411 468 183 239 2.41e-11 65.9

Find 176 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGGTTTAAGTGGGGTAAT+TGG 0.192042 tig0025956:+63029 None:intergenic
TTCGGTTTAAGTGGGGTAAT+TGG 0.192042 tig0025956:+63281 None:intergenic
ACTACAAATGTAGAAGTTTC+TGG 0.203501 tig0025956:-61149 Msa1426250:CDS
AGTCTTTGATGACTTGTTTC+GGG 0.228480 tig0025956:+61002 None:intergenic
TCGGTTTAAGTGGGGTAATT+GGG 0.264814 tig0025956:+63030 None:intergenic
TCGGTTTAAGTGGGGTAATT+GGG 0.264814 tig0025956:+63282 None:intergenic
GGTAGTGATGGTTGGGATAC+TGG 0.266171 tig0025956:+62935 None:intergenic
GGAAGGGATGGTGGTGCTTG+AGG 0.267804 tig0025956:+63142 None:intergenic
GAAGGGATGGTGGTGCTTGA+GGG 0.285161 tig0025956:+63143 None:intergenic
TGGATGTGGAGACCCTTTAA+TGG 0.287579 tig0025956:+62117 None:intergenic
TGGAGGTGGAGTTTCAAAAT+TGG 0.294278 tig0025956:+63400 None:intergenic
AAACTTGGAATCGCCTAATA+TGG 0.320459 tig0025956:-60935 Msa1426250:CDS
AGGGAGTAGGAGTAGTGGTT+GGG 0.321158 tig0025956:+62910 None:intergenic
TGGGGCTGAGTTATTCTCTT+TGG 0.321183 tig0025956:+63049 None:intergenic
TGGGGCTGAGTTATTCTCTT+TGG 0.321183 tig0025956:+63301 None:intergenic
TAGTGAAGATGGTGGTAGTG+GGG 0.325049 tig0025956:+62679 None:intergenic
CTCCAATGGACAATGATGAT+TGG 0.334060 tig0025956:-63478 None:intergenic
CAAAATTTAAATCAAGAAAA+AGG 0.335426 tig0025956:-62320 Msa1426250:intron
GAAGGGATGGTGGTGCTTGA+GGG 0.338016 tig0025956:+62891 None:intergenic
TAGTCTTTGATGACTTGTTT+CGG 0.341304 tig0025956:+61001 None:intergenic
TTGAGGGGATATGATGTTAT+TGG 0.344141 tig0025956:+63361 None:intergenic
GTAGTTGATTGCGCTTTCTC+AGG 0.345960 tig0025956:+62400 None:intergenic
ATGAAATCATTCGGTTTAAG+TGG 0.356968 tig0025956:+63020 None:intergenic
ATGAAATCATTCGGTTTAAG+TGG 0.356968 tig0025956:+63272 None:intergenic
GTTGTAGGGATAGTGGTTGT+AGG 0.360959 tig0025956:+62654 None:intergenic
GGATATGATGTTATTGGTTG+TGG 0.366736 tig0025956:+63367 None:intergenic
ACTTATGTCGTGACATATAA+AGG 0.369771 tig0025956:-61078 Msa1426250:CDS
TACACTTCATTGTTACGGTT+TGG 0.377794 tig0025956:+62507 None:intergenic
AGTTCTTACCGATACGTCTA+TGG 0.380944 tig0025956:-60872 Msa1426250:CDS
AGGGATGGTGGTGCTTGAGG+GGG 0.385352 tig0025956:+63145 None:intergenic
GGATGTGGAGACCCTTTAAT+GGG 0.385486 tig0025956:+62118 None:intergenic
TTGCGATTTCACTCCGGGCA+TGG 0.385512 tig0025956:+62472 None:intergenic
GGAAGGGATGGTGGTGCTTG+AGG 0.390413 tig0025956:+62890 None:intergenic
GTTGGGGTGGTAGTGATGGT+TGG 0.392907 tig0025956:+62927 None:intergenic
GTTGGGGTGGTAGTGATGGT+TGG 0.392907 tig0025956:+63179 None:intergenic
GTTGTGGTGGTTAGAGTTGG+AGG 0.394427 tig0025956:+63383 None:intergenic
GGTAGTGAAGATGGTGGTAG+TGG 0.395152 tig0025956:+62677 None:intergenic
AGGGTGGCGAATGTGGTTGT+AGG 0.396346 tig0025956:+62639 None:intergenic
TTTGTTCGAGGTAGTAGAAA+TGG 0.396886 tig0025956:+62378 None:intergenic
AAGGGATGGTGGTGCTTGAG+GGG 0.398824 tig0025956:+63144 None:intergenic
AACGGCAACATAGCCATATT+AGG 0.400299 tig0025956:+60922 None:intergenic
GTTGGGATACTAGTGATAGT+AGG 0.406363 tig0025956:+63197 None:intergenic
AAACATGACACCTATGTTAG+AGG 0.407154 tig0025956:-60809 Msa1426250:CDS
AGGGGTTGTTGTGGAATTGA+TGG 0.409578 tig0025956:+62420 None:intergenic
AGAGGTGGGGATAGTGGTGT+TGG 0.411378 tig0025956:+62719 None:intergenic
TCCAATGGACAATGATGATT+GGG 0.413894 tig0025956:-63477 None:intergenic
TTGGTTGTGGTGGTTAGAGT+TGG 0.415176 tig0025956:+63380 None:intergenic
CGCTTTCTCAGGGGTTGTTG+TGG 0.415304 tig0025956:+62411 None:intergenic
GTTGGGATACTGGTGATAGT+AGG 0.415858 tig0025956:+62945 None:intergenic
GTGGTGATGGTAGGTATGAT+AGG 0.418388 tig0025956:+62603 None:intergenic
TTGGGATACTAGTGATAGTA+GGG 0.418845 tig0025956:+63198 None:intergenic
TTGGGGTGGTAGTGATGGTT+GGG 0.421087 tig0025956:+62928 None:intergenic
TTGGGGTGGTAGTGATGGTT+GGG 0.421087 tig0025956:+63180 None:intergenic
CTACAAATGTAGAAGTTTCT+GGG 0.423709 tig0025956:-61148 Msa1426250:CDS
GAGGGAGTAGGAGTAGTGGT+TGG 0.426227 tig0025956:+62909 None:intergenic
TAGAAGTTTCTGGGATTGTT+CGG 0.428728 tig0025956:-61139 Msa1426250:CDS
GGGGTGGGGATAGTTGTTGT+TGG 0.434582 tig0025956:+62966 None:intergenic
GATGGTAGAGGTGGGGATAG+TGG 0.437887 tig0025956:+62713 None:intergenic
GGTAATGTGGGAAGGGATGG+TGG 0.443946 tig0025956:+62881 None:intergenic
GGTAATGTGGGAAGGGATGG+TGG 0.443946 tig0025956:+63133 None:intergenic
GATAGTAGGGGTGGGGATAG+TGG 0.445875 tig0025956:+63211 None:intergenic
AGTGGTTGGGGTGGTAGTGA+TGG 0.446309 tig0025956:+62923 None:intergenic
AGTGGTTGGGGTGGTAGTGA+TGG 0.446309 tig0025956:+63175 None:intergenic
TCAAGCTCTATGAAATCATT+CGG 0.447306 tig0025956:+63011 None:intergenic
TCAAGCTCTATGAAATCATT+CGG 0.447306 tig0025956:+63263 None:intergenic
ACTTGAGCCTCGTTGCTCTC+TGG 0.448771 tig0025956:+60892 None:intergenic
TATGTTAGAGGATCTCTTGA+TGG 0.450163 tig0025956:-60797 Msa1426250:CDS
GGGAGTAGGAGTAGTGGTTG+GGG 0.451422 tig0025956:+62911 None:intergenic
TTGGGATACTGGTGATAGTA+GGG 0.453252 tig0025956:+62946 None:intergenic
GAGGTGGGGATAGTGGTGTT+GGG 0.454610 tig0025956:+62720 None:intergenic
AAACATACACTTCATTGTTA+CGG 0.454894 tig0025956:+62502 None:intergenic
ACGAACCTTCAGAAGATTCA+TGG 0.454895 tig0025956:-62165 Msa1426250:CDS
GGGGTGGGGATAGTGGTTGT+TGG 0.455018 tig0025956:+63218 None:intergenic
TGTTGGGAAATTAGAAAAGA+TGG 0.456325 tig0025956:+62812 None:intergenic
TTAATCAGAACTCCGTTGTT+GGG 0.460189 tig0025956:-61029 Msa1426250:CDS
TTGTAGGGATAGTGGTTGTA+GGG 0.462214 tig0025956:+62655 None:intergenic
TGGTGATGGTAGGTATGATA+GGG 0.464626 tig0025956:+62604 None:intergenic
GTTGGTTGCGATTTCACTCC+GGG 0.465774 tig0025956:+62467 None:intergenic
AGGGGTAGTGGTGGTGAGGG+TGG 0.470018 tig0025956:+62623 None:intergenic
AGTGAAGATGGTGGTAGTGG+GGG 0.472105 tig0025956:+62680 None:intergenic
GGTGGTGAGGGTGGCGAATG+TGG 0.473258 tig0025956:+62632 None:intergenic
TTATGCATTGGCAAAGAAGC+TGG 0.473516 tig0025956:-60976 Msa1426250:CDS
GTAGTGAAGATGGTGGTAGT+GGG 0.473726 tig0025956:+62678 None:intergenic
GGTGGTAATGTGGGAAGGGA+TGG 0.475750 tig0025956:+62878 None:intergenic
GGTGGTAATGTGGGAAGGGA+TGG 0.475750 tig0025956:+63130 None:intergenic
TGATAGGGGTAGTGGTGGTG+AGG 0.478349 tig0025956:+62619 None:intergenic
TAGTTGATTGCGCTTTCTCA+GGG 0.481392 tig0025956:+62401 None:intergenic
TTGCGCAAATGTCAGAAACT+TGG 0.482429 tig0025956:-60950 Msa1426250:CDS
TATGATGTTATTGGTTGTGG+TGG 0.483931 tig0025956:+63370 None:intergenic
TCTAACATAGGTGTCATGTT+TGG 0.484082 tig0025956:+60811 None:intergenic
CGGTTTAAGTGGGGTAATTG+GGG 0.485800 tig0025956:+63031 None:intergenic
CGGTTTAAGTGGGGTAATTG+GGG 0.485800 tig0025956:+63283 None:intergenic
GTTGTTGTTCGATGAGTGTT+GGG 0.487764 tig0025956:+62796 None:intergenic
TACTGGTGATAGTAGGGGTG+GGG 0.488721 tig0025956:+62952 None:intergenic
GGTAGGAGTAGTGGTTGGGG+TGG 0.493221 tig0025956:+63166 None:intergenic
GTTAATCAGAACTCCGTTGT+TGG 0.498948 tig0025956:-61030 Msa1426250:CDS
GTGGTGGTTAGAGTTGGAGG+TGG 0.500554 tig0025956:+63386 None:intergenic
ATAGAGCACCACTATTGATC+TGG 0.502409 tig0025956:+60767 None:intergenic
ATGGTGGTGCTTGAGGGAGT+AGG 0.504780 tig0025956:+62897 None:intergenic
GGTTGTAGGGGTAGTGAAGA+TGG 0.506539 tig0025956:+62668 None:intergenic
GATACTGGTGATAGTAGGGG+TGG 0.507596 tig0025956:+62950 None:intergenic
TTGCTCTCTGGCTGATCTAA+CGG 0.509856 tig0025956:+60904 None:intergenic
ACGGAGTTCTGATTAACTGC+TGG 0.513492 tig0025956:+61036 None:intergenic
TGAAATCATTCGGTTTAAGT+GGG 0.513951 tig0025956:+63021 None:intergenic
TGAAATCATTCGGTTTAAGT+GGG 0.513951 tig0025956:+63273 None:intergenic
TGTTGTTGTTCGATGAGTGT+TGG 0.514177 tig0025956:+62795 None:intergenic
GTTGTTGTGGAATTGATGGA+TGG 0.514803 tig0025956:+62424 None:intergenic
TGATGGTATTGGTGGTAATG+TGG 0.520234 tig0025956:+62868 None:intergenic
TGATGGTATTGGTGGTAATG+TGG 0.520234 tig0025956:+63120 None:intergenic
GATAGGGGTAGTGGTGGTGA+GGG 0.522610 tig0025956:+62620 None:intergenic
AGGTGGAGTTTCAAAATTGG+TGG 0.528008 tig0025956:+63403 None:intergenic
GGTGTGAGCAGTGATGGTAG+AGG 0.528684 tig0025956:+62701 None:intergenic
CATGCCCATGAATCTTCTGA+AGG 0.531817 tig0025956:+62160 None:intergenic
GTGAGCAGTGATGGTAGAGG+TGG 0.533809 tig0025956:+62704 None:intergenic
GGGTGGCGAATGTGGTTGTA+GGG 0.537742 tig0025956:+62640 None:intergenic
TGTTGGTTGCGATTTCACTC+CGG 0.540502 tig0025956:+62466 None:intergenic
ATCAAGAGATCCTCTAACAT+AGG 0.542974 tig0025956:+60799 None:intergenic
TGAGCAGTGATGGTAGAGGT+GGG 0.544379 tig0025956:+62705 None:intergenic
GGTAGGTATGATAGGGGTAG+TGG 0.546289 tig0025956:+62611 None:intergenic
GATGGTATTGGTGGTAATGT+GGG 0.548603 tig0025956:+62869 None:intergenic
GATGGTATTGGTGGTAATGT+GGG 0.548603 tig0025956:+63121 None:intergenic
TCCCAATCATCATTGTCCAT+TGG 0.550956 tig0025956:+63476 None:intergenic
ATTAGTCGTTGTAGTGGTGA+TGG 0.553401 tig0025956:+62590 None:intergenic
ATACTAGTGATAGTAGGGGT+GGG 0.555078 tig0025956:+63203 None:intergenic
TACTAGTGATAGTAGGGGTG+GGG 0.555369 tig0025956:+63204 None:intergenic
ATGTTTCTGAACCCCATGCC+CGG 0.557908 tig0025956:-62485 Msa1426250:CDS
CTTCAGAAGATTCATGGGCA+TGG 0.559542 tig0025956:-62159 Msa1426250:CDS
GACTTGAACCAGATCAATAG+TGG 0.561347 tig0025956:-60775 Msa1426250:CDS
GCGATTTCACTCCGGGCATG+GGG 0.562469 tig0025956:+62474 None:intergenic
ACTAGTGTTAGTGATGGTAT+TGG 0.562993 tig0025956:+62857 None:intergenic
GGAGTTTCAAAATTGGTGGT+AGG 0.564205 tig0025956:+63407 None:intergenic
AGGTGGGGATAGTGGTGTTG+GGG 0.565500 tig0025956:+62721 None:intergenic
CATGGCACACAAATGTCACT+TGG 0.574104 tig0025956:+62197 None:intergenic
ACTAGTGTTAGTGATGGTAT+TGG 0.575832 tig0025956:+63109 None:intergenic
AGTAGGAGTAGTGGTTGGGG+TGG 0.584381 tig0025956:+62914 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.584498 tig0025956:-62114 Msa1426250:intron
CGAACCTTCAGAAGATTCAT+GGG 0.585253 tig0025956:-62164 Msa1426250:CDS
ATCATCAAAGCATGGAGGTG+TGG 0.586562 tig0025956:+63082 None:intergenic
AAACTAAAATCATCAAAGCA+TGG 0.588328 tig0025956:+63074 None:intergenic
AAACTAAAATCATCAAAGCA+TGG 0.588328 tig0025956:+63326 None:intergenic
TCAAAGACTAGATTATGCAT+TGG 0.588584 tig0025956:-60988 Msa1426250:CDS
GCTTGAGGGAGTAGGAGTAG+TGG 0.588951 tig0025956:+62905 None:intergenic
AAGGTTCGTCTTCCCTCACA+TGG 0.591333 tig0025956:+62179 None:intergenic
GAATGTGGTTGTAGGGATAG+TGG 0.594762 tig0025956:+62647 None:intergenic
ATCATCAAAGCATGGAGGTG+TGG 0.594915 tig0025956:+63334 None:intergenic
ATGAAAACTAGTGTTAGTGA+TGG 0.596957 tig0025956:+62851 None:intergenic
GGTGATGGTAGGTATGATAG+GGG 0.597368 tig0025956:+62605 None:intergenic
GAATGCACAATCAAAACTGT+TGG 0.598794 tig0025956:+62449 None:intergenic
ATACTGGTGATAGTAGGGGT+GGG 0.601151 tig0025956:+62951 None:intergenic
AGTGTTAGTGATGGTATTGG+TGG 0.606164 tig0025956:+62860 None:intergenic
AGTGTTAGTGATGGTATTGG+TGG 0.606164 tig0025956:+63112 None:intergenic
GTATTGGTGGTAATGTGGGA+AGG 0.610190 tig0025956:+62873 None:intergenic
GTATTGGTGGTAATGTGGGA+AGG 0.610190 tig0025956:+63125 None:intergenic
GATACTAGTGATAGTAGGGG+TGG 0.616026 tig0025956:+63202 None:intergenic
ATTGGTGGTAGGAGTAGCAA+AGG 0.621937 tig0025956:+63418 None:intergenic
ACTAGTATTAGTCGTTGTAG+TGG 0.622593 tig0025956:+62584 None:intergenic
TATTGGTGGTAATGTGGGAA+GGG 0.622832 tig0025956:+62874 None:intergenic
TATTGGTGGTAATGTGGGAA+GGG 0.622832 tig0025956:+63126 None:intergenic
GACATTTGTGTGCCATGTGA+GGG 0.624856 tig0025956:-62191 Msa1426250:CDS
CTTTGATGACTTGTTTCGGG+AGG 0.626610 tig0025956:+61005 None:intergenic
TGTAGGGATAGTGGTTGTAG+GGG 0.630150 tig0025956:+62656 None:intergenic
TGGGATACTGGTGATAGTAG+GGG 0.637481 tig0025956:+62947 None:intergenic
GAAATCATTCGGTTTAAGTG+GGG 0.641149 tig0025956:+63022 None:intergenic
GAAATCATTCGGTTTAAGTG+GGG 0.641149 tig0025956:+63274 None:intergenic
GTCGTTGTAGTGGTGATGGT+AGG 0.642975 tig0025956:+62594 None:intergenic
TGACATTTGTGTGCCATGTG+AGG 0.643817 tig0025956:-62192 Msa1426250:CDS
AGGTATGATAGGGGTAGTGG+TGG 0.649146 tig0025956:+62614 None:intergenic
GTTTCGGGAGGTCCCAACAA+CGG 0.649755 tig0025956:+61017 None:intergenic
AGTTGATTGCGCTTTCTCAG+GGG 0.662828 tig0025956:+62402 None:intergenic
GGTAAAACTAGTGTTAGTGA+TGG 0.671690 tig0025956:+63103 None:intergenic
TGTAGGGGTAGTGAAGATGG+TGG 0.689397 tig0025956:+62671 None:intergenic
GAGCAGTGATGGTAGAGGTG+GGG 0.706368 tig0025956:+62706 None:intergenic
TGGGATACTAGTGATAGTAG+GGG 0.723289 tig0025956:+63199 None:intergenic
CTAAAATCATCAAAGCATGG+AGG 0.732238 tig0025956:+63077 None:intergenic
CTAAAATCATCAAAGCATGG+AGG 0.732238 tig0025956:+63329 None:intergenic
AGATCAGCCAGAGAGCAACG+AGG 0.772309 tig0025956:-60899 Msa1426250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATATAATAAATAAT+AGG + tig0025956:61951-61970 None:intergenic 0.0%
!!! TTCACTATAAATAAATTTTA+CGG + tig0025956:62572-62591 None:intergenic 10.0%
!! AAATTTATTTATAGTGAAAT+TGG - tig0025956:62574-62593 Msa1426250:CDS 10.0%
!!! CTTTTTCTTGATTTAAATTT+TGG + tig0025956:61914-61933 None:intergenic 15.0%
!! CAAAATTTAAATCAAGAAAA+AGG - tig0025956:61912-61931 Msa1426250:intron 15.0%
!!! ATGAAATGTATAATGATTTT+AGG + tig0025956:61993-62012 None:intergenic 15.0%
!! TATCAAAATAAGGATTAAAA+TGG + tig0025956:62612-62631 None:intergenic 15.0%
!!! GATTTTATTCTATCAAAATA+AGG + tig0025956:62622-62641 None:intergenic 15.0%
!! TATATACATTTATATGTGTA+TGG - tig0025956:62987-63006 Msa1426250:CDS 15.0%
!!! AAATATACCACTTTTTCTTT+TGG + tig0025956:62718-62737 None:intergenic 20.0%
!! TAAATAAAGTGAAAGATCTT+AGG + tig0025956:62862-62881 None:intergenic 20.0%
!!! ATATCAATGAATGTTTTTGT+AGG - tig0025956:63056-63075 Msa1426250:CDS 20.0%
! AAACTAAAATCATCAAAGCA+TGG + tig0025956:60909-60928 None:intergenic 25.0%
! AAACTAAAATCATCAAAGCA+TGG + tig0025956:60909-60928 None:intergenic 25.0%
! AAACATACACTTCATTGTTA+CGG + tig0025956:61733-61752 None:intergenic 25.0%
!! AAAAATCTAGAATTTGTTCG+AGG + tig0025956:61869-61888 None:intergenic 25.0%
! TTAACTACAATTCTTTCCAA+TGG + tig0025956:62272-62291 None:intergenic 25.0%
!!! ATGTACTGTTAATTGCTTTT+TGG - tig0025956:62433-62452 Msa1426250:CDS 25.0%
! GGATTAAAATGGATATTGAA+GGG + tig0025956:62601-62620 None:intergenic 25.0%
! AGGATTAAAATGGATATTGA+AGG + tig0025956:62602-62621 None:intergenic 25.0%
! CTAATATGAATAAAGTTTGC+AGG - tig0025956:62671-62690 Msa1426250:CDS 25.0%
! ATAAAGACCAAAAGAAAAAG+TGG - tig0025956:62708-62727 Msa1426250:CDS 25.0%
! AAAAGAGAGGATATATTTGT+AGG - tig0025956:62762-62781 Msa1426250:CDS 25.0%
! AAAGAGAGGATATATTTGTA+GGG - tig0025956:62763-62782 Msa1426250:CDS 25.0%
! TATTAAACAGTACGATTTAG+CGG - tig0025956:63017-63036 Msa1426250:CDS 25.0%
TGAAATCATTCGGTTTAAGT+GGG + tig0025956:60962-60981 None:intergenic 30.0%
ATGAAATCATTCGGTTTAAG+TGG + tig0025956:60963-60982 None:intergenic 30.0%
TCAAGCTCTATGAAATCATT+CGG + tig0025956:60972-60991 None:intergenic 30.0%
TGAAATCATTCGGTTTAAGT+GGG + tig0025956:60962-60981 None:intergenic 30.0%
ATGAAATCATTCGGTTTAAG+TGG + tig0025956:60963-60982 None:intergenic 30.0%
TCAAGCTCTATGAAATCATT+CGG + tig0025956:60972-60991 None:intergenic 30.0%
ATGAAAACTAGTGTTAGTGA+TGG + tig0025956:61384-61403 None:intergenic 30.0%
TGTTGGGAAATTAGAAAAGA+TGG + tig0025956:61423-61442 None:intergenic 30.0%
GTTTACTGAGCTAGAAAAAA+TGG - tig0025956:61475-61494 Msa1426250:intron 30.0%
!!! GGTTTTTTCGAATGTGTATT+TGG + tig0025956:61893-61912 None:intergenic 30.0%
TATGGAGAAAAACCCATTAA+AGG - tig0025956:62102-62121 Msa1426250:intron 30.0%
ATGGAGAAAAACCCATTAAA+GGG - tig0025956:62103-62122 Msa1426250:intron 30.0%
CGTTTAATGCATAAAAACCT+TGG + tig0025956:62138-62157 None:intergenic 30.0%
! CACTATTTAAAACTCTAGGT+GGG + tig0025956:62227-62246 None:intergenic 30.0%
! TCACTATTTAAAACTCTAGG+TGG + tig0025956:62228-62247 None:intergenic 30.0%
! CGATCACTATTTAAAACTCT+AGG + tig0025956:62231-62250 None:intergenic 30.0%
!!! AGTTTTAAATAGTGATCGCA+AGG - tig0025956:62233-62252 Msa1426250:intron 30.0%
TACAATTCTTTCCAATGGTA+AGG + tig0025956:62267-62286 None:intergenic 30.0%
CAAAAAATGATACATCTGCA+TGG + tig0025956:62476-62495 None:intergenic 30.0%
!!! GTTTAGTCCCTCTATTTTTT+GGG - tig0025956:62497-62516 Msa1426250:CDS 30.0%
TGTGTTTCCCAAAAAATAGA+GGG + tig0025956:62507-62526 None:intergenic 30.0%
TTGTGTTTCCCAAAAAATAG+AGG + tig0025956:62508-62527 None:intergenic 30.0%
ACAATTACACCTTTGATCTT+TGG - tig0025956:62524-62543 Msa1426250:CDS 30.0%
AAGGAAGAAAAAGAAAAGAG+AGG - tig0025956:62749-62768 Msa1426250:CDS 30.0%
ACTACAAATGTAGAAGTTTC+TGG - tig0025956:63083-63102 Msa1426250:CDS 30.0%
CTACAAATGTAGAAGTTTCT+GGG - tig0025956:63084-63103 Msa1426250:CDS 30.0%
ACTTATGTCGTGACATATAA+AGG - tig0025956:63154-63173 Msa1426250:CDS 30.0%
!! TAGTCTTTGATGACTTGTTT+CGG + tig0025956:63234-63253 None:intergenic 30.0%
TCAAAGACTAGATTATGCAT+TGG - tig0025956:63244-63263 Msa1426250:CDS 30.0%
! TATGATGTTATTGGTTGTGG+TGG + tig0025956:60865-60884 None:intergenic 35.0%
! GGATATGATGTTATTGGTTG+TGG + tig0025956:60868-60887 None:intergenic 35.0%
TTGAGGGGATATGATGTTAT+TGG + tig0025956:60874-60893 None:intergenic 35.0%
CTAAAATCATCAAAGCATGG+AGG + tig0025956:60906-60925 None:intergenic 35.0%
GAAATCATTCGGTTTAAGTG+GGG + tig0025956:60961-60980 None:intergenic 35.0%
TTGGGATACTAGTGATAGTA+GGG + tig0025956:61037-61056 None:intergenic 35.0%
ACTAGTGTTAGTGATGGTAT+TGG + tig0025956:61126-61145 None:intergenic 35.0%
GGTAAAACTAGTGTTAGTGA+TGG + tig0025956:61132-61151 None:intergenic 35.0%
CTAAAATCATCAAAGCATGG+AGG + tig0025956:60906-60925 None:intergenic 35.0%
GAAATCATTCGGTTTAAGTG+GGG + tig0025956:60961-60980 None:intergenic 35.0%
ACTAGTGTTAGTGATGGTAT+TGG + tig0025956:61126-61145 None:intergenic 35.0%
ACTAGTATTAGTCGTTGTAG+TGG + tig0025956:61651-61670 None:intergenic 35.0%
TACACTTCATTGTTACGGTT+TGG + tig0025956:61728-61747 None:intergenic 35.0%
GAATGCACAATCAAAACTGT+TGG + tig0025956:61786-61805 None:intergenic 35.0%
TTTGTTCGAGGTAGTAGAAA+TGG + tig0025956:61857-61876 None:intergenic 35.0%
TCATGGGCATGGAAAAAATA+TGG - tig0025956:62084-62103 Msa1426250:intron 35.0%
ATGCATAAAAACCTTGGATG+TGG + tig0025956:62132-62151 None:intergenic 35.0%
GTAGTTAAATGCAACATACG+TGG - tig0025956:62284-62303 Msa1426250:intron 35.0%
!!! CGTTTAGTCCCTCTATTTTT+TGG - tig0025956:62496-62515 Msa1426250:CDS 35.0%
CATCAAAGACCAAAGATCAA+AGG + tig0025956:62536-62555 None:intergenic 35.0%
TCTTACACTGAGTGACATAT+TGG + tig0025956:62934-62953 None:intergenic 35.0%
ATATGTCACTCAGTGTAAGA+CGG - tig0025956:62934-62953 Msa1426250:CDS 35.0%
!! TAGAAGTTTCTGGGATTGTT+CGG - tig0025956:63093-63112 Msa1426250:CDS 35.0%
TTAATCAGAACTCCGTTGTT+GGG - tig0025956:63203-63222 Msa1426250:CDS 35.0%
!! AGTCTTTGATGACTTGTTTC+GGG + tig0025956:63233-63252 None:intergenic 35.0%
AAACTTGGAATCGCCTAATA+TGG - tig0025956:63297-63316 Msa1426250:CDS 35.0%
CAAAAACTCCATAGACGTAT+CGG + tig0025956:63371-63390 None:intergenic 35.0%
! GATACGTCTATGGAGTTTTT+GGG - tig0025956:63370-63389 Msa1426250:CDS 35.0%
! TCTAACATAGGTGTCATGTT+TGG + tig0025956:63424-63443 None:intergenic 35.0%
AAACATGACACCTATGTTAG+AGG - tig0025956:63423-63442 Msa1426250:CDS 35.0%
ATCAAGAGATCCTCTAACAT+AGG + tig0025956:63436-63455 None:intergenic 35.0%
TATGTTAGAGGATCTCTTGA+TGG - tig0025956:63435-63454 Msa1426250:CDS 35.0%
! GGAGTTTCAAAATTGGTGGT+AGG + tig0025956:60828-60847 None:intergenic 40.0%
AGGTGGAGTTTCAAAATTGG+TGG + tig0025956:60832-60851 None:intergenic 40.0%
TGGAGGTGGAGTTTCAAAAT+TGG + tig0025956:60835-60854 None:intergenic 40.0%
TCGGTTTAAGTGGGGTAATT+GGG + tig0025956:60953-60972 None:intergenic 40.0%
TTCGGTTTAAGTGGGGTAAT+TGG + tig0025956:60954-60973 None:intergenic 40.0%
ATACTAGTGATAGTAGGGGT+GGG + tig0025956:61032-61051 None:intergenic 40.0%
TGGGATACTAGTGATAGTAG+GGG + tig0025956:61036-61055 None:intergenic 40.0%
GTTGGGATACTAGTGATAGT+AGG + tig0025956:61038-61057 None:intergenic 40.0%
! TATTGGTGGTAATGTGGGAA+GGG + tig0025956:61109-61128 None:intergenic 40.0%
!! GATGGTATTGGTGGTAATGT+GGG + tig0025956:61114-61133 None:intergenic 40.0%
!! TGATGGTATTGGTGGTAATG+TGG + tig0025956:61115-61134 None:intergenic 40.0%
! AGTGTTAGTGATGGTATTGG+TGG + tig0025956:61123-61142 None:intergenic 40.0%
TCGGTTTAAGTGGGGTAATT+GGG + tig0025956:60953-60972 None:intergenic 40.0%
TTCGGTTTAAGTGGGGTAAT+TGG + tig0025956:60954-60973 None:intergenic 40.0%
TTGGGATACTGGTGATAGTA+GGG + tig0025956:61289-61308 None:intergenic 40.0%
! TATTGGTGGTAATGTGGGAA+GGG + tig0025956:61109-61128 None:intergenic 40.0%
!! GATGGTATTGGTGGTAATGT+GGG + tig0025956:61114-61133 None:intergenic 40.0%
!! TGATGGTATTGGTGGTAATG+TGG + tig0025956:61115-61134 None:intergenic 40.0%
! AGTGTTAGTGATGGTATTGG+TGG + tig0025956:61123-61142 None:intergenic 40.0%
! GTTGTTGTTCGATGAGTGTT+GGG + tig0025956:61439-61458 None:intergenic 40.0%
! TGTTGTTGTTCGATGAGTGT+TGG + tig0025956:61440-61459 None:intergenic 40.0%
! TTGTAGGGATAGTGGTTGTA+GGG + tig0025956:61580-61599 None:intergenic 40.0%
!! TGGTGATGGTAGGTATGATA+GGG + tig0025956:61631-61650 None:intergenic 40.0%
ATTAGTCGTTGTAGTGGTGA+TGG + tig0025956:61645-61664 None:intergenic 40.0%
GTTGTTGTGGAATTGATGGA+TGG + tig0025956:61811-61830 None:intergenic 40.0%
TAGTTGATTGCGCTTTCTCA+GGG + tig0025956:61834-61853 None:intergenic 40.0%
ACGAACCTTCAGAAGATTCA+TGG - tig0025956:62067-62086 Msa1426250:intron 40.0%
CGAACCTTCAGAAGATTCAT+GGG - tig0025956:62068-62087 Msa1426250:intron 40.0%
CTTTCCAATGGTAAGGATCA+TGG + tig0025956:62260-62279 None:intergenic 40.0%
! AACATACGTGGTCTTAATCG+CGG - tig0025956:62296-62315 Msa1426250:intron 40.0%
GTTAATCAGAACTCCGTTGT+TGG - tig0025956:63202-63221 Msa1426250:CDS 40.0%
! TTATGCATTGGCAAAGAAGC+TGG - tig0025956:63256-63275 Msa1426250:CDS 40.0%
TTGCGCAAATGTCAGAAACT+TGG - tig0025956:63282-63301 Msa1426250:CDS 40.0%
AACGGCAACATAGCCATATT+AGG + tig0025956:63313-63332 None:intergenic 40.0%
AGTTCTTACCGATACGTCTA+TGG - tig0025956:63360-63379 Msa1426250:CDS 40.0%
! CGATACGTCTATGGAGTTTT+TGG - tig0025956:63369-63388 Msa1426250:CDS 40.0%
GACTTGAACCAGATCAATAG+TGG - tig0025956:63457-63476 Msa1426250:CDS 40.0%
! ATAGAGCACCACTATTGATC+TGG + tig0025956:63468-63487 None:intergenic 40.0%
! ATTGGTGGTAGGAGTAGCAA+AGG + tig0025956:60817-60836 None:intergenic 45.0%
TTGGTTGTGGTGGTTAGAGT+TGG + tig0025956:60855-60874 None:intergenic 45.0%
!! ATGGAGGTGTGGTGTTTTGA+GGG + tig0025956:60890-60909 None:intergenic 45.0%
ATCATCAAAGCATGGAGGTG+TGG + tig0025956:60901-60920 None:intergenic 45.0%
TGGGGCTGAGTTATTCTCTT+TGG + tig0025956:60934-60953 None:intergenic 45.0%
CGGTTTAAGTGGGGTAATTG+GGG + tig0025956:60952-60971 None:intergenic 45.0%
TACTAGTGATAGTAGGGGTG+GGG + tig0025956:61031-61050 None:intergenic 45.0%
GATACTAGTGATAGTAGGGG+TGG + tig0025956:61033-61052 None:intergenic 45.0%
! GTATTGGTGGTAATGTGGGA+AGG + tig0025956:61110-61129 None:intergenic 45.0%
ATCATCAAAGCATGGAGGTG+TGG + tig0025956:60901-60920 None:intergenic 45.0%
TGGGGCTGAGTTATTCTCTT+TGG + tig0025956:60934-60953 None:intergenic 45.0%
CGGTTTAAGTGGGGTAATTG+GGG + tig0025956:60952-60971 None:intergenic 45.0%
ATACTGGTGATAGTAGGGGT+GGG + tig0025956:61284-61303 None:intergenic 45.0%
TGGGATACTGGTGATAGTAG+GGG + tig0025956:61288-61307 None:intergenic 45.0%
GTTGGGATACTGGTGATAGT+AGG + tig0025956:61290-61309 None:intergenic 45.0%
! GTATTGGTGGTAATGTGGGA+AGG + tig0025956:61110-61129 None:intergenic 45.0%
! TAGTGAAGATGGTGGTAGTG+GGG + tig0025956:61556-61575 None:intergenic 45.0%
! GTAGTGAAGATGGTGGTAGT+GGG + tig0025956:61557-61576 None:intergenic 45.0%
! TGTAGGGATAGTGGTTGTAG+GGG + tig0025956:61579-61598 None:intergenic 45.0%
! GTTGTAGGGATAGTGGTTGT+AGG + tig0025956:61581-61600 None:intergenic 45.0%
GAATGTGGTTGTAGGGATAG+TGG + tig0025956:61588-61607 None:intergenic 45.0%
! GGTGATGGTAGGTATGATAG+GGG + tig0025956:61630-61649 None:intergenic 45.0%
!! GTGGTGATGGTAGGTATGAT+AGG + tig0025956:61632-61651 None:intergenic 45.0%
! TGTTGGTTGCGATTTCACTC+CGG + tig0025956:61769-61788 None:intergenic 45.0%
AGGGGTTGTTGTGGAATTGA+TGG + tig0025956:61815-61834 None:intergenic 45.0%
AGTTGATTGCGCTTTCTCAG+GGG + tig0025956:61833-61852 None:intergenic 45.0%
GTAGTTGATTGCGCTTTCTC+AGG + tig0025956:61835-61854 None:intergenic 45.0%
CATGGCACACAAATGTCACT+TGG + tig0025956:62038-62057 None:intergenic 45.0%
TGACATTTGTGTGCCATGTG+AGG - tig0025956:62040-62059 Msa1426250:intron 45.0%
GACATTTGTGTGCCATGTGA+GGG - tig0025956:62041-62060 Msa1426250:intron 45.0%
CATGCCCATGAATCTTCTGA+AGG + tig0025956:62075-62094 None:intergenic 45.0%
CTTCAGAAGATTCATGGGCA+TGG - tig0025956:62073-62092 Msa1426250:intron 45.0%
GGATGTGGAGACCCTTTAAT+GGG + tig0025956:62117-62136 None:intergenic 45.0%
TGGATGTGGAGACCCTTTAA+TGG + tig0025956:62118-62137 None:intergenic 45.0%
TTAAAGGGTCTCCACATCCA+AGG - tig0025956:62118-62137 Msa1426250:CDS 45.0%
AGGACCATGATCCTTACCAT+TGG - tig0025956:62253-62272 Msa1426250:intron 45.0%
! GTGTCGCTCATTGCAAAGAA+TGG + tig0025956:62363-62382 None:intergenic 45.0%
! ACGGAGTTCTGATTAACTGC+TGG + tig0025956:63199-63218 None:intergenic 45.0%
!! CTTTGATGACTTGTTTCGGG+AGG + tig0025956:63230-63249 None:intergenic 45.0%
TTGCTCTCTGGCTGATCTAA+CGG + tig0025956:63331-63350 None:intergenic 45.0%
!!! GTATATTTTTTTTAAAAAAA+AGG - tig0025956:62730-62749 Msa1426250:CDS 5.0%
GTTGTGGTGGTTAGAGTTGG+AGG + tig0025956:60852-60871 None:intergenic 50.0%
!! TGGAGGTGTGGTGTTTTGAG+GGG + tig0025956:60889-60908 None:intergenic 50.0%
!! CATGGAGGTGTGGTGTTTTG+AGG + tig0025956:60891-60910 None:intergenic 50.0%
! TTGGGGTGGTAGTGATGGTT+GGG + tig0025956:61055-61074 None:intergenic 50.0%
TACTGGTGATAGTAGGGGTG+GGG + tig0025956:61283-61302 None:intergenic 50.0%
GATACTGGTGATAGTAGGGG+TGG + tig0025956:61285-61304 None:intergenic 50.0%
GGTAGTGATGGTTGGGATAC+TGG + tig0025956:61048-61067 None:intergenic 50.0%
! TTGGGGTGGTAGTGATGGTT+GGG + tig0025956:61055-61074 None:intergenic 50.0%
AGGGAGTAGGAGTAGTGGTT+GGG + tig0025956:61325-61344 None:intergenic 50.0%
TGAGCAGTGATGGTAGAGGT+GGG + tig0025956:61530-61549 None:intergenic 50.0%
!! AGTGAAGATGGTGGTAGTGG+GGG + tig0025956:61555-61574 None:intergenic 50.0%
! GGTAGTGAAGATGGTGGTAG+TGG + tig0025956:61558-61577 None:intergenic 50.0%
TGTAGGGGTAGTGAAGATGG+TGG + tig0025956:61564-61583 None:intergenic 50.0%
GGTTGTAGGGGTAGTGAAGA+TGG + tig0025956:61567-61586 None:intergenic 50.0%
! AGGTATGATAGGGGTAGTGG+TGG + tig0025956:61621-61640 None:intergenic 50.0%
GGTAGGTATGATAGGGGTAG+TGG + tig0025956:61624-61643 None:intergenic 50.0%
!! GTCGTTGTAGTGGTGATGGT+AGG + tig0025956:61641-61660 None:intergenic 50.0%
! ATGTTTCTGAACCCCATGCC+CGG - tig0025956:61747-61766 Msa1426250:intron 50.0%
GTTGGTTGCGATTTCACTCC+GGG + tig0025956:61768-61787 None:intergenic 50.0%
AAGGTTCGTCTTCCCTCACA+TGG + tig0025956:62056-62075 None:intergenic 50.0%
CTCTAGGTGGGTGTGTGTTT+AGG + tig0025956:62215-62234 None:intergenic 50.0%
GTGGTGGTTAGAGTTGGAGG+TGG + tig0025956:60849-60868 None:intergenic 55.0%
GATAGTAGGGGTGGGGATAG+TGG + tig0025956:61024-61043 None:intergenic 55.0%
! GTTGGGGTGGTAGTGATGGT+TGG + tig0025956:61056-61075 None:intergenic 55.0%
! AGTGGTTGGGGTGGTAGTGA+TGG + tig0025956:61060-61079 None:intergenic 55.0%
AGGGGGTAGGAGTAGTGGTT+GGG + tig0025956:61073-61092 None:intergenic 55.0%
! AAGGGATGGTGGTGCTTGAG+GGG + tig0025956:61091-61110 None:intergenic 55.0%
! GAAGGGATGGTGGTGCTTGA+GGG + tig0025956:61092-61111 None:intergenic 55.0%
GGTAATGTGGGAAGGGATGG+TGG + tig0025956:61102-61121 None:intergenic 55.0%
! GGTGGTAATGTGGGAAGGGA+TGG + tig0025956:61105-61124 None:intergenic 55.0%
GGGGTGGGGATAGTTGTTGT+TGG + tig0025956:61269-61288 None:intergenic 55.0%
! GTTGGGGTGGTAGTGATGGT+TGG + tig0025956:61056-61075 None:intergenic 55.0%
! AGTGGTTGGGGTGGTAGTGA+TGG + tig0025956:61060-61079 None:intergenic 55.0%
AGTAGGAGTAGTGGTTGGGG+TGG + tig0025956:61321-61340 None:intergenic 55.0%
GGGAGTAGGAGTAGTGGTTG+GGG + tig0025956:61324-61343 None:intergenic 55.0%
GAGGGAGTAGGAGTAGTGGT+TGG + tig0025956:61326-61345 None:intergenic 55.0%
GCTTGAGGGAGTAGGAGTAG+TGG + tig0025956:61330-61349 None:intergenic 55.0%
! ATGGTGGTGCTTGAGGGAGT+AGG + tig0025956:61338-61357 None:intergenic 55.0%
! GAAGGGATGGTGGTGCTTGA+GGG + tig0025956:61092-61111 None:intergenic 55.0%
GGTAATGTGGGAAGGGATGG+TGG + tig0025956:61102-61121 None:intergenic 55.0%
! GGTGGTAATGTGGGAAGGGA+TGG + tig0025956:61105-61124 None:intergenic 55.0%
! AGGTGGGGATAGTGGTGTTG+GGG + tig0025956:61514-61533 None:intergenic 55.0%
! GAGGTGGGGATAGTGGTGTT+GGG + tig0025956:61515-61534 None:intergenic 55.0%
! AGAGGTGGGGATAGTGGTGT+TGG + tig0025956:61516-61535 None:intergenic 55.0%
GATGGTAGAGGTGGGGATAG+TGG + tig0025956:61522-61541 None:intergenic 55.0%
GAGCAGTGATGGTAGAGGTG+GGG + tig0025956:61529-61548 None:intergenic 55.0%
GTGAGCAGTGATGGTAGAGG+TGG + tig0025956:61531-61550 None:intergenic 55.0%
GGTGTGAGCAGTGATGGTAG+AGG + tig0025956:61534-61553 None:intergenic 55.0%
GGGTGGCGAATGTGGTTGTA+GGG + tig0025956:61595-61614 None:intergenic 55.0%
AGGGTGGCGAATGTGGTTGT+AGG + tig0025956:61596-61615 None:intergenic 55.0%
! GATAGGGGTAGTGGTGGTGA+GGG + tig0025956:61615-61634 None:intergenic 55.0%
! TGATAGGGGTAGTGGTGGTG+AGG + tig0025956:61616-61635 None:intergenic 55.0%
TGCGATTTCACTCCGGGCAT+GGG + tig0025956:61762-61781 None:intergenic 55.0%
TTGCGATTTCACTCCGGGCA+TGG + tig0025956:61763-61782 None:intergenic 55.0%
CGCTTTCTCAGGGGTTGTTG+TGG + tig0025956:61824-61843 None:intergenic 55.0%
GTTTCGGGAGGTCCCAACAA+CGG + tig0025956:63218-63237 None:intergenic 55.0%
AGATCAGCCAGAGAGCAACG+AGG - tig0025956:63333-63352 Msa1426250:CDS 55.0%
ACTTGAGCCTCGTTGCTCTC+TGG + tig0025956:63343-63362 None:intergenic 55.0%
! GGGGTGGGGATAGTGGTTGT+TGG + tig0025956:61017-61036 None:intergenic 60.0%
GGTAGGAGTAGTGGTTGGGG+TGG + tig0025956:61069-61088 None:intergenic 60.0%
GGGGGTAGGAGTAGTGGTTG+GGG + tig0025956:61072-61091 None:intergenic 60.0%
GAGGGGGTAGGAGTAGTGGT+TGG + tig0025956:61074-61093 None:intergenic 60.0%
GCTTGAGGGGGTAGGAGTAG+TGG + tig0025956:61078-61097 None:intergenic 60.0%
! ATGGTGGTGCTTGAGGGGGT+AGG + tig0025956:61086-61105 None:intergenic 60.0%
! AGGGATGGTGGTGCTTGAGG+GGG + tig0025956:61090-61109 None:intergenic 60.0%
! GGAAGGGATGGTGGTGCTTG+AGG + tig0025956:61093-61112 None:intergenic 60.0%
! GGAAGGGATGGTGGTGCTTG+AGG + tig0025956:61093-61112 None:intergenic 60.0%
! AGTGGGGGTGTGAGCAGTGA+TGG + tig0025956:61540-61559 None:intergenic 60.0%
GCGATTTCACTCCGGGCATG+GGG + tig0025956:61761-61780 None:intergenic 60.0%
GGTGGTGAGGGTGGCGAATG+TGG + tig0025956:61603-61622 None:intergenic 65.0%
! AGGGGTAGTGGTGGTGAGGG+TGG + tig0025956:61612-61631 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0025956 gene 60759 63495 60759 ID=Msa1426250;Name=Msa1426250
tig0025956 mRNA 60759 63495 60759 ID=Msa1426250-mRNA-1;Parent=Msa1426250;Name=Msa1426250-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|569
tig0025956 exon 62321 63495 62321 ID=Msa1426250-mRNA-1:exon:4765;Parent=Msa1426250-mRNA-1
tig0025956 exon 62115 62231 62115 ID=Msa1426250-mRNA-1:exon:4764;Parent=Msa1426250-mRNA-1
tig0025956 exon 60759 61176 60759 ID=Msa1426250-mRNA-1:exon:4763;Parent=Msa1426250-mRNA-1
tig0025956 CDS 62321 63495 62321 ID=Msa1426250-mRNA-1:cds;Parent=Msa1426250-mRNA-1
tig0025956 CDS 62115 62231 62115 ID=Msa1426250-mRNA-1:cds;Parent=Msa1426250-mRNA-1
tig0025956 CDS 60759 61176 60759 ID=Msa1426250-mRNA-1:cds;Parent=Msa1426250-mRNA-1
Gene Sequence

>Msa1426250

ATGGACAATGATGATTGGGATCTCTTTTCTATTGTTCGTAGCTGCAAAGCTACTACCTTTGCTACTCCTACCACCAATTTTGAAACTCCACCTCCAACTCTAACCACCACAACCAATAACATCATATCCCCTCAAAACACCACACCTCCATGCTTTGATGATTTTAGTTTTACCAAAGAGAATAACTCAGCCCCAATTACCCCACTTAAACCGAATGATTTCATAGAGCTTGACAAATTGATAATCAGTTTCAATCCAACAACCACTATCCCCACCCCTACTATCACTAGTATCCCAACCATCACTACCACCCCAACCACTACTCCTACCCCCTCAAGCACCACCATCCCTTCCCACATTACCACCAATACCATCACTAACACTAGTTTTACCACACCTCCATGCTTTGATGATTTTAGTTTTACCAAAGAGAATAACTCAGCCCCAATTACCCCACTTAAACCGAATGATTTCATAGAGCTTGACAAATTGATAATCAGTTTCAATCCAACAACAACTATCCCCACCCCTACTATCACCAGTATCCCAACCATCACTACCACCCCAACCACTACTCCTACTCCCTCAAGCACCACCATCCCTTCCCACATTACCACCAATACCATCACTAACACTAGTTTTCATGATTCTAATCAAAATTCCATCTTTTCTAATTTCCCAACACTCATCGAACAACAACAACTGCAACTAAATGAGTTTACTGAGCTAGAAAAAATGGTACTTAAATTCTACCCCAACACCACTATCCCCACCTCTACCATCACTGCTCACACCCCCACTACCACCATCTTCACTACCCCTACAACCACTATCCCTACAACCACATTCGCCACCCTCACCACCACTACCCCTATCATACCTACCATCACCACTACAACGACTAATACTAGTGTTTGCAAAACAAATCAATATCTTACAATACTCGTCGAAACACAACAGAATCAACCAAACCGTAACAATGAAGTGTATGTTTCTGAACCCCATGCCCGGAGTGAAATCGCAACCAACAGTTTTGATTGTGCATTCAACCATCCATCAATTCCACAACAACCCCTGAGAAAGCGCAATCAACTACCATTTCTACTACCTCGAACAAATTCTAGATTTTTACCAAATACACATTCGAAAAAACCAAAATTTAAATCAAGAAAAAGAAAAAATAATAACCAAGTGACATTTGTGTGCCATGTGAGGGAAGACGAACCTTCAGAAGATTCATGGGCATGGAAAAAATATGGAGAAAAACCCATTAAAGGGTCTCCACATCCAAGGAGCTACTACAAATGTAGAAGTTTCTGGGATTGTTCGGCAAAAAAACTAGTAGAGAAAAGCAAAACATGTGAGAACACTTATGTCGTGACATATAAAGGAGAACACAATCATAAAAAACCAGCAGTTAATCAGAACTCCGTTGTTGGGACCTCCCGAAACAAGTCATCAAAGACTAGATTATGCATTGGCAAAGAAGCTGGATCTTGCGCAAATGTCAGAAACTTGGAATCGCCTAATATGGCTATGTTGCCGTTAGATCAGCCAGAGAGCAACGAGGCTCAAGTTCTTACCGATACGTCTATGGAGTTTTTGGGAAGCTCTGATGACGATGTCATTTTGATACCAAACATGACACCTATGTTAGAGGATCTCTTGATGGACTTGAACCAGATCAATAGTGGTGCTCTATTCCCTTAG
Protein sequence

>Msa1426250

MDNDDWDLFSIVRSCKATTFATPTTNFETPPPTLTTTTNNIISPQNTTPPCFDDFSFTKENNSAPITPLKPNDFIELDKLIISFNPTTTIPTPTITSIPTITTTPTTTPTPSSTTIPSHITTNTITNTSFTTPPCFDDFSFTKENNSAPITPLKPNDFIELDKLIISFNPTTTIPTPTITSIPTITTTPTTTPTPSSTTIPSHITTNTITNTSFHDSNQNSIFSNFPTLIEQQQLQLNEFTELEKMVLKFYPNTTIPTSTITAHTPTTTIFTTPTTTIPTTTFATLTTTTPIIPTITTTTTNTSVCKTNQYLTILVETQQNQPNRNNEVYVSEPHARSEIATNSFDCAFNHPSIPQQPLRKRNQLPFLLPRTNSRFLPNTHSKKPKFKSRKRKNNNQVTFVCHVREDEPSEDSWAWKKYGEKPIKGSPHPRSYYKCRSFWDCSAKKLVEKSKTCENTYVVTYKGEHNHKKPAVNQNSVVGTSRNKSSKTRLCIGKEAGSCANVRNLESPNMAMLPLDQPESNEAQVLTDTSMEFLGSSDDDVILIPNMTPMLEDLLMDLNQINSGALFP*