AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1427300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427300 MtrunA17_Chr4g0061791 86.901 313 11 1 1 313 288 570 0.0 543
Msa1427300 MtrunA17_Chr5g0420701 37.931 319 136 8 1 309 66 332 7.84e-55 181
Msa1427300 MtrunA17_Chr7g0220371 35.401 274 120 9 2 264 73 300 7.36e-31 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427300 AT5G08130.3 39.773 264 92 8 1 264 178 374 3.12e-39 142
Msa1427300 AT5G08130.1 39.394 264 94 8 1 264 178 375 1.29e-38 141
Msa1427300 AT5G08130.7 40.152 264 91 8 1 264 298 494 3.99e-38 142
Msa1427300 AT5G08130.5 39.773 264 92 8 1 264 302 498 4.05e-38 142
Msa1427300 AT1G69010.1 37.313 268 102 10 1 264 67 272 1.09e-37 136
Msa1427300 AT5G08130.2 39.773 264 93 8 1 264 298 495 1.30e-37 140
Msa1427300 AT5G08130.4 39.773 264 93 8 1 264 298 495 1.30e-37 140
Msa1427300 AT5G08130.6 39.394 264 94 8 1 264 298 495 1.39e-37 140
Msa1427300 AT5G08130.8 39.394 264 94 8 1 264 302 499 1.43e-37 140
Msa1427300 AT5G38860.1 32.197 264 109 7 2 262 56 252 1.88e-25 103
Msa1427300 AT5G38860.2 39.130 138 63 3 2 137 56 174 5.04e-21 90.1

Find 96 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCATTTGTGGATAGAAAT+TGG 0.227383 tig0026181:-66373 Msa1427300:CDS
TGGGTTGAAGTTAAGAAATT+TGG 0.265205 tig0026181:+66692 None:intergenic
TTCTACTGAAGGTTGATAAT+TGG 0.293752 tig0026181:+66604 None:intergenic
CTCCACCTAAACCTCCGATT+TGG 0.310407 tig0026181:+66672 None:intergenic
AAGTTGTGTTGGACATGATA+TGG 0.317695 tig0026181:-66410 Msa1427300:CDS
AAATTTGGTTGGGAAGAAAT+AGG 0.321463 tig0026181:+66707 None:intergenic
CTTAGTTTGTTGGGATCCTT+AGG 0.342893 tig0026181:-65241 Msa1427300:three_prime_UTR
TGAATTTACCTCTAACAAAA+AGG 0.343203 tig0026181:+67480 None:intergenic
ATCTTCCTTTGATTCTTAGA+AGG 0.344596 tig0026181:+65483 None:intergenic
AACATTGCTGTTTGCTTCAA+AGG 0.352246 tig0026181:-66864 Msa1427300:CDS
TCAACCCAAATCGGAGGTTT+AGG 0.354794 tig0026181:-66677 Msa1427300:CDS
TCAAGCCAGATTCTACATTA+TGG 0.356652 tig0026181:+66795 None:intergenic
ATGTAGTGTCTTAGTTTGTT+GGG 0.370568 tig0026181:-65250 Msa1427300:three_prime_UTR
ACCATCTCCCCAACAACTCT+TGG 0.370776 tig0026181:-66824 Msa1427300:CDS
CATGTAGTGTCTTAGTTTGT+TGG 0.372497 tig0026181:-65251 Msa1427300:three_prime_UTR
ACACATGCCTTGCAGAGTTC+AGG 0.377988 tig0026181:-65742 Msa1427300:CDS
GGGGTATTTATTTCCTGTGC+TGG 0.378434 tig0026181:-65306 Msa1427300:three_prime_UTR
TTTATCACAGGCTAGCATTT+CGG 0.379312 tig0026181:-65713 Msa1427300:CDS
CGGATGATCTATTGTCTTCA+TGG 0.382464 tig0026181:+66760 None:intergenic
CCATCTCCCCAACAACTCTT+GGG 0.386641 tig0026181:-66823 Msa1427300:CDS
TCCACCTAAACCTCCGATTT+GGG 0.392555 tig0026181:+66673 None:intergenic
CATCCCCTAGTGAGCATTTG+TGG 0.408785 tig0026181:-66384 Msa1427300:CDS
CAAAGTAAATGTGCTATACT+TGG 0.413343 tig0026181:-65785 Msa1427300:intron
CACATTGGCATTGGTATGTC+TGG 0.415087 tig0026181:+65655 None:intergenic
TTCTTAACTTCAACCCAAAT+CGG 0.419263 tig0026181:-66686 Msa1427300:CDS
TTGTTTACTTGACCTTTCCT+TGG 0.424889 tig0026181:-64941 Msa1427300:three_prime_UTR
AATCTTGAAAAGTATCTCAT+AGG 0.441412 tig0026181:+64963 None:intergenic
CTGGCTCGTTATTCCAACCT+TGG 0.443215 tig0026181:+67330 None:intergenic
CAATCTCGTGGCGCAAATGG+TGG 0.446698 tig0026181:-66902 Msa1427300:CDS
ATTTCGGTGCAAATTGAACT+TGG 0.449045 tig0026181:-65697 Msa1427300:CDS
CGCTCTGGCTCTAGAGTCAC+TGG 0.450006 tig0026181:-65459 Msa1427300:CDS
GGTATCCATAATGTAGAATC+TGG 0.451732 tig0026181:-66800 Msa1427300:CDS
GGTTGGGAAGAAATAGGACT+TGG 0.461704 tig0026181:+66713 None:intergenic
CCCAAGAGTTGTTGGGGAGA+TGG 0.463978 tig0026181:+66823 None:intergenic
TCTATCCACAAATGCTCACT+AGG 0.466076 tig0026181:+66379 None:intergenic
GCTGTAAATTAGTAAACTCT+TGG 0.466747 tig0026181:-65115 Msa1427300:three_prime_UTR
GCTATACTTGGACAGGTTGT+TGG 0.468086 tig0026181:-65773 Msa1427300:CDS
TGAAGTTAAGAAATTTGGTT+GGG 0.473775 tig0026181:+66697 None:intergenic
GCTAATGGCACCTCCTTCAA+TGG 0.484672 tig0026181:+66532 None:intergenic
CTAAATATATATAATTATAG+AGG 0.485547 tig0026181:-65182 Msa1427300:three_prime_UTR
TTTGAAGCAAACAGCAATGT+TGG 0.487282 tig0026181:+66866 None:intergenic
GAGAAAGAGCTCACCATTGA+AGG 0.497721 tig0026181:-66545 Msa1427300:CDS
CGAGATTGAAAGCTTTCTAC+TGG 0.498307 tig0026181:+66917 None:intergenic
TTTCAATCTCGTGGCGCAAA+TGG 0.499033 tig0026181:-66905 Msa1427300:CDS
ATGAAGGTTCATTCCAAGGT+TGG 0.500331 tig0026181:-67343 Msa1427300:CDS
TGCTGGATTATTATCCTGCA+TGG 0.504277 tig0026181:-65289 Msa1427300:three_prime_UTR
GCCTTGTTTGTTATTCCAGA+CGG 0.512631 tig0026181:+66740 None:intergenic
TAAATGTGCTATACTTGGAC+AGG 0.513308 tig0026181:-65780 Msa1427300:CDS
TTGAAGTTAAGAAATTTGGT+TGG 0.519525 tig0026181:+66696 None:intergenic
GTACACACTTGATATGCTAA+TGG 0.523913 tig0026181:+66517 None:intergenic
AAGACAATAGATCATCCGTC+TGG 0.528087 tig0026181:-66755 Msa1427300:CDS
AATGCCAATGTGTGTTTCCA+AGG 0.530445 tig0026181:-65644 Msa1427300:intron
TTGGAAACACACATTGGCAT+TGG 0.530708 tig0026181:+65646 None:intergenic
TTCAGGAGTGGATTTATCAC+AGG 0.531531 tig0026181:-65725 Msa1427300:CDS
AATCAAAGGAAGATGCGCTC+TGG 0.532036 tig0026181:-65474 Msa1427300:CDS
TTTATTAGCTATCACAATGA+GGG 0.532937 tig0026181:+64993 None:intergenic
TTCCACACAGTGATCAAAAG+AGG 0.537014 tig0026181:-67513 Msa1427300:CDS
AAGATCCTTCTAAGAATCAA+AGG 0.539351 tig0026181:-65488 Msa1427300:CDS
GAGAAGGTTCATAAGTATGA+AGG 0.541707 tig0026181:-67359 Msa1427300:CDS
GCAAACAGCAATGTTGGAGA+TGG 0.547592 tig0026181:+66872 None:intergenic
AAGTATGAAGGTTCATTCCA+AGG 0.551145 tig0026181:-67347 Msa1427300:CDS
AATCTCGTGGCGCAAATGGT+GGG 0.551190 tig0026181:-66901 Msa1427300:CDS
CTATCCACAAATGCTCACTA+GGG 0.558631 tig0026181:+66380 None:intergenic
CAAAGCAGAGAAATAATGAC+AGG 0.558692 tig0026181:-66940 Msa1427300:intron
AGTTTACTAATTTACAGCAA+TGG 0.566532 tig0026181:+65120 None:intergenic
ACCCAAATCGGAGGTTTAGG+TGG 0.566780 tig0026181:-66674 Msa1427300:CDS
TATAAATAATGAGCTACGGA+AGG 0.570947 tig0026181:+65024 None:intergenic
AAAACAGCTTGTATCAGTAT+GGG 0.573845 tig0026181:+65156 None:intergenic
ATAGTCTGAGATTCTACTGA+AGG 0.581921 tig0026181:+66593 None:intergenic
AGGCTATAAATAATGAGCTA+CGG 0.582243 tig0026181:+65020 None:intergenic
TGCCTTGCAGAGTTCAGGAG+TGG 0.584050 tig0026181:-65737 Msa1427300:CDS
ACAGTCAATCTCATCCATGC+AGG 0.585678 tig0026181:+65275 None:intergenic
ATAAATAATGAGCTACGGAA+GGG 0.588340 tig0026181:+65025 None:intergenic
GATAGAAGCAATCCAAGGAA+AGG 0.589019 tig0026181:+64929 None:intergenic
GTAGAAAGCTTTCAATCTCG+TGG 0.593016 tig0026181:-66914 Msa1427300:CDS
GCAGGATAATAATCCAGCAC+AGG 0.595198 tig0026181:+65293 None:intergenic
CATCTCCCCAACAACTCTTG+GGG 0.595919 tig0026181:-66822 Msa1427300:CDS
TAAATAATGAGCTACGGAAG+GGG 0.597042 tig0026181:+65026 None:intergenic
ATCCACTCCTGAACTCTGCA+AGG 0.600178 tig0026181:+65735 None:intergenic
TTAACTTCAACCCAAATCGG+AGG 0.605590 tig0026181:-66683 Msa1427300:CDS
TGAGGAAAGAATGAATGTAA+CGG 0.614056 tig0026181:+65379 None:intergenic
ATCTCCCCAACAACTCTTGG+GGG 0.615224 tig0026181:-66821 Msa1427300:CDS
GTGGATAGAAATTGGAGATG+TGG 0.617637 tig0026181:-66365 Msa1427300:CDS
GCATTGGTATGTCTGGTCTG+AGG 0.617749 tig0026181:+65662 None:intergenic
ATAGAAATTGGAGATGTGGC+AGG 0.617759 tig0026181:-66361 Msa1427300:intron
ATATCAAGTGTGTACTCACA+AGG 0.619435 tig0026181:-66509 Msa1427300:CDS
AAAGAGCTCACCATTGAAGG+AGG 0.629369 tig0026181:-66542 Msa1427300:CDS
TATACCTTGGAAACACACAT+TGG 0.634078 tig0026181:+65640 None:intergenic
TCCACACAGTGATCAAAAGA+GGG 0.643944 tig0026181:-67512 Msa1427300:CDS
GCTATCACAATGAGGGTGTG+AGG 0.644440 tig0026181:+65000 None:intergenic
ACAGCTTGTATCAGTATGGG+AGG 0.645446 tig0026181:+65159 None:intergenic
ATCTGGCTTGAGCACAACAA+CGG 0.650337 tig0026181:-66783 Msa1427300:CDS
GAATTGATAGAAGCAATCCA+AGG 0.672992 tig0026181:+64924 None:intergenic
TATCCACAAATGCTCACTAG+GGG 0.675975 tig0026181:+66381 None:intergenic
TCCGTCTGGAATAACAAACA+AGG 0.684989 tig0026181:-66741 Msa1427300:CDS
TATCAAGTGTGTACTCACAA+GGG 0.699426 tig0026181:-66508 Msa1427300:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTGATAAAAATTATTG+AGG + tig0026181:67105-67124 None:intergenic 10.0%
!!! TAATTTTTATCAATTATTTG+GGG - tig0026181:67108-67127 Msa1427300:intron 10.0%
!!! TTTTAAAATTAATTTTTGGA+AGG - tig0026181:67136-67155 Msa1427300:intron 10.0%
!!! TTTAAAATTAATTTTTGGAA+GGG - tig0026181:67137-67156 Msa1427300:intron 10.0%
!! CTAAATATATATAATTATAG+AGG - tig0026181:67281-67300 Msa1427300:intron 10.0%
!!! ATTTTTTTTAACATTGATTT+TGG - tig0026181:67386-67405 Msa1427300:CDS 10.0%
!! GTTTAATCTAAATTATTTGT+AGG - tig0026181:65055-65074 Msa1427300:three_prime_UTR 15.0%
!! TCATGTTTATAAAGAAATAA+AGG + tig0026181:65336-65355 None:intergenic 15.0%
!! AATTTAAATCCATTAACAAT+AGG + tig0026181:65460-65479 None:intergenic 15.0%
!! GAATATATAAATACATTAGT+AGG + tig0026181:66180-66199 None:intergenic 15.0%
!! ATAAATATTTCCACAATATA+TGG - tig0026181:66484-66503 Msa1427300:intron 15.0%
!! AAATGATAAATGTTGTAATT+AGG - tig0026181:66612-66631 Msa1427300:CDS 15.0%
!!! TTAAAATTAATTTTTGGAAG+GGG - tig0026181:67138-67157 Msa1427300:intron 15.0%
!! ACAACTTCAAAATAGTATAA+AGG + tig0026181:66040-66059 None:intergenic 20.0%
!! TAAGAAGCAAATTGAAATTA+TGG + tig0026181:66349-66368 None:intergenic 20.0%
!! ATGTAAACTATAACATAAAG+AGG + tig0026181:66391-66410 None:intergenic 20.0%
!! TGTATAATGAATTGTTTGAA+TGG + tig0026181:66450-66469 None:intergenic 20.0%
!! TATAAAAAGACTTTACAAGA+GGG + tig0026181:66862-66881 None:intergenic 20.0%
!! TTATAAAAAGACTTTACAAG+AGG + tig0026181:66863-66882 None:intergenic 20.0%
!! ACAGATTAGATAATAATACA+TGG + tig0026181:66931-66950 None:intergenic 20.0%
!!! ATGATGCATATTATTTTGTT+GGG + tig0026181:67253-67272 None:intergenic 20.0%
!!! AATGATGCATATTATTTTGT+TGG + tig0026181:67254-67273 None:intergenic 20.0%
!!! TTTTTTAACATTGATTTTGG+TGG - tig0026181:67389-67408 Msa1427300:CDS 20.0%
! TGAATTTACCTCTAACAAAA+AGG + tig0026181:64986-65005 None:intergenic 25.0%
! TTGTTAGAGGTAAATTCATA+CGG - tig0026181:64988-65007 Msa1427300:three_prime_UTR 25.0%
! CAAAATAATAGCTTCATTGA+TGG - tig0026181:65220-65239 Msa1427300:three_prime_UTR 25.0%
! TTCTTTATAAACATGATCAG+AGG - tig0026181:65340-65359 Msa1427300:three_prime_UTR 25.0%
! TCTTTATAAACATGATCAGA+GGG - tig0026181:65341-65360 Msa1427300:three_prime_UTR 25.0%
!! GAAAAATGTGAATCAGTTTT+TGG + tig0026181:65499-65518 None:intergenic 25.0%
! TGAAGTTAAGAAATTTGGTT+GGG + tig0026181:65769-65788 None:intergenic 25.0%
! TTGAAGTTAAGAAATTTGGT+TGG + tig0026181:65770-65789 None:intergenic 25.0%
!!! ATACTATTTTGAAGTTGTGT+TGG - tig0026181:66042-66061 Msa1427300:intron 25.0%
!!! AAACAGTTTTTTAATCCTCA+TGG + tig0026181:66209-66228 None:intergenic 25.0%
! GAATTTCGAGAAAAGAAAAT+GGG + tig0026181:66588-66607 None:intergenic 25.0%
! AGAATTTCGAGAAAAGAAAA+TGG + tig0026181:66589-66608 None:intergenic 25.0%
! AATGCTAACAAAAACCAATT+TGG + tig0026181:66641-66660 None:intergenic 25.0%
! TAATACATGGTTTCATAATG+TGG + tig0026181:66918-66937 None:intergenic 25.0%
!!! TGATGCATATTATTTTGTTG+GGG + tig0026181:67252-67271 None:intergenic 25.0%
! AGTTTACTAATTTACAGCAA+TGG + tig0026181:67346-67365 None:intergenic 25.0%
! TTTATTAGCTATCACAATGA+GGG + tig0026181:67473-67492 None:intergenic 25.0%
!! TTTTATTAGCTATCACAATG+AGG + tig0026181:67474-67493 None:intergenic 25.0%
!!! AATCTTGAAAAGTATCTCAT+AGG + tig0026181:67503-67522 None:intergenic 25.0%
!!! CATTCACTTTTTACAAGAGA+AGG - tig0026181:65088-65107 Msa1427300:three_prime_UTR 30.0%
AATCTTTCACAGCTTAAGAT+AGG + tig0026181:65192-65211 None:intergenic 30.0%
TTTCTCATAAAATGATCAGG+AGG + tig0026181:65263-65282 None:intergenic 30.0%
ACGTTTCTCATAAAATGATC+AGG + tig0026181:65266-65285 None:intergenic 30.0%
CAATCAAAACTATTAGGTCT+AGG + tig0026181:65307-65326 None:intergenic 30.0%
!!! ACCTAATAGTTTTGATTGTC+TGG - tig0026181:65309-65328 Msa1427300:three_prime_UTR 30.0%
ACCAGACAATCAAAACTATT+AGG + tig0026181:65313-65332 None:intergenic 30.0%
ATCACATAGCCTATTGTTAA+TGG - tig0026181:65448-65467 Msa1427300:CDS 30.0%
!! AATGTGAATCAGTTTTTGGA+TGG + tig0026181:65495-65514 None:intergenic 30.0%
AAATTTGGTTGGGAAGAAAT+AGG + tig0026181:65759-65778 None:intergenic 30.0%
! TGGGTTGAAGTTAAGAAATT+TGG + tig0026181:65774-65793 None:intergenic 30.0%
TTCTTAACTTCAACCCAAAT+CGG - tig0026181:65777-65796 Msa1427300:CDS 30.0%
!! TTCTACTGAAGGTTGATAAT+TGG + tig0026181:65862-65881 None:intergenic 30.0%
ATTGAATTGTTAGATGACTG+TGG + tig0026181:66151-66170 None:intergenic 30.0%
TGAATTGTTTGAATGGAACA+GGG + tig0026181:66443-66462 None:intergenic 30.0%
ATGAATTGTTTGAATGGAAC+AGG + tig0026181:66444-66463 None:intergenic 30.0%
TTGTAATTAGGATGCCAAAT+TGG - tig0026181:66624-66643 Msa1427300:CDS 30.0%
CAAAGTAAATGTGCTATACT+TGG - tig0026181:66678-66697 Msa1427300:CDS 30.0%
CAATTTGAGCAAATATACCT+TGG + tig0026181:66839-66858 None:intergenic 30.0%
! AAGATCCTTCTAAGAATCAA+AGG - tig0026181:66975-66994 Msa1427300:intron 30.0%
ATCTTCCTTTGATTCTTAGA+AGG + tig0026181:66983-67002 None:intergenic 30.0%
TGAGGAAAGAATGAATGTAA+CGG + tig0026181:67087-67106 None:intergenic 30.0%
!! ATGTAGTGTCTTAGTTTGTT+GGG - tig0026181:67213-67232 Msa1427300:intron 30.0%
!! ATGCATATTATTTTGTTGGG+GGG + tig0026181:67250-67269 None:intergenic 30.0%
!! GATGCATATTATTTTGTTGG+GGG + tig0026181:67251-67270 None:intergenic 30.0%
AAAACAGCTTGTATCAGTAT+GGG + tig0026181:67310-67329 None:intergenic 30.0%
AAAAACAGCTTGTATCAGTA+TGG + tig0026181:67311-67330 None:intergenic 30.0%
GCTGTAAATTAGTAAACTCT+TGG - tig0026181:67348-67367 Msa1427300:CDS 30.0%
ATAAATAATGAGCTACGGAA+GGG + tig0026181:67441-67460 None:intergenic 30.0%
TATAAATAATGAGCTACGGA+AGG + tig0026181:67442-67461 None:intergenic 30.0%
AGGCTATAAATAATGAGCTA+CGG + tig0026181:67446-67465 None:intergenic 30.0%
!! CAAAGCATCCTTTTTGTTAG+AGG - tig0026181:64975-64994 Msa1427300:three_prime_UTR 35.0%
TAGAGGTAAATTCATACGGT+TGG - tig0026181:64992-65011 Msa1427300:three_prime_UTR 35.0%
GAGAAGGTTCATAAGTATGA+AGG - tig0026181:65104-65123 Msa1427300:three_prime_UTR 35.0%
AAGTATGAAGGTTCATTCCA+AGG - tig0026181:65116-65135 Msa1427300:three_prime_UTR 35.0%
! TTCAAGTTTGATGTCCTTGT+TGG - tig0026181:65371-65390 Msa1427300:three_prime_UTR 35.0%
!! AGATGCTTGTCTTGTTTAAG+TGG - tig0026181:65411-65430 Msa1427300:CDS 35.0%
!! GATGCTTGTCTTGTTTAAGT+GGG - tig0026181:65412-65431 Msa1427300:CDS 35.0%
CAAAGCAGAGAAATAATGAC+AGG - tig0026181:65523-65542 Msa1427300:intron 35.0%
! TTTGAAGCAAACAGCAATGT+TGG + tig0026181:65600-65619 None:intergenic 35.0%
!! AACATTGCTGTTTGCTTCAA+AGG - tig0026181:65599-65618 Msa1427300:intron 35.0%
GGTATCCATAATGTAGAATC+TGG - tig0026181:65663-65682 Msa1427300:CDS 35.0%
TCAAGCCAGATTCTACATTA+TGG + tig0026181:65671-65690 None:intergenic 35.0%
! ATAGTCTGAGATTCTACTGA+AGG + tig0026181:65873-65892 None:intergenic 35.0%
! TTTCTCTTTCAGCTTTTCAC+TGG + tig0026181:65904-65923 None:intergenic 35.0%
GTACACACTTGATATGCTAA+TGG + tig0026181:65949-65968 None:intergenic 35.0%
ATATCAAGTGTGTACTCACA+AGG - tig0026181:65954-65973 Msa1427300:intron 35.0%
TATCAAGTGTGTACTCACAA+GGG - tig0026181:65955-65974 Msa1427300:intron 35.0%
AAGTTGTGTTGGACATGATA+TGG - tig0026181:66053-66072 Msa1427300:intron 35.0%
GAGCATTTGTGGATAGAAAT+TGG - tig0026181:66090-66109 Msa1427300:intron 35.0%
AATTGTTAGATGACTGTGGA+TGG + tig0026181:66147-66166 None:intergenic 35.0%
!!! GTTTTTTAATCCTCATGGCA+GGG + tig0026181:66204-66223 None:intergenic 35.0%
!!! AGTTTTTTAATCCTCATGGC+AGG + tig0026181:66205-66224 None:intergenic 35.0%
AGTGAAGTAAGTCCAAGTTT+AGG + tig0026181:66261-66280 None:intergenic 35.0%
!! CTAGGTTGATCCATATATTG+TGG + tig0026181:66497-66516 None:intergenic 35.0%
! AAGTTTTCCAACAACATGCT+AGG + tig0026181:66515-66534 None:intergenic 35.0%
TAAATGTGCTATACTTGGAC+AGG - tig0026181:66683-66702 Msa1427300:CDS 35.0%
TTTATCACAGGCTAGCATTT+CGG - tig0026181:66750-66769 Msa1427300:CDS 35.0%
! ATTTCGGTGCAAATTGAACT+TGG - tig0026181:66766-66785 Msa1427300:CDS 35.0%
TATACCTTGGAAACACACAT+TGG + tig0026181:66826-66845 None:intergenic 35.0%
!! CATGTAGTGTCTTAGTTTGT+TGG - tig0026181:67212-67231 Msa1427300:intron 35.0%
TAAATAATGAGCTACGGAAG+GGG + tig0026181:67440-67459 None:intergenic 35.0%
TTGTTTACTTGACCTTTCCT+TGG - tig0026181:67522-67541 Msa1427300:CDS 35.0%
TTCCACACAGTGATCAAAAG+AGG - tig0026181:64950-64969 Msa1427300:three_prime_UTR 40.0%
TCCACACAGTGATCAAAAGA+GGG - tig0026181:64951-64970 Msa1427300:three_prime_UTR 40.0%
ATGAAGGTTCATTCCAAGGT+TGG - tig0026181:65120-65139 Msa1427300:three_prime_UTR 40.0%
! GAAAGTGTGGTATTTACCCA+TGG + tig0026181:65170-65189 None:intergenic 40.0%
CAGCTTAAGATAGGAAAGTG+TGG + tig0026181:65183-65202 None:intergenic 40.0%
TTGCTCAGTCAATTCCAACA+AGG + tig0026181:65388-65407 None:intergenic 40.0%
CGAGATTGAAAGCTTTCTAC+TGG + tig0026181:65549-65568 None:intergenic 40.0%
GTAGAAAGCTTTCAATCTCG+TGG - tig0026181:65549-65568 Msa1427300:intron 40.0%
CGGATGATCTATTGTCTTCA+TGG + tig0026181:65706-65725 None:intergenic 40.0%
AAGACAATAGATCATCCGTC+TGG - tig0026181:65708-65727 Msa1427300:CDS 40.0%
! TCCGTCTGGAATAACAAACA+AGG - tig0026181:65722-65741 Msa1427300:CDS 40.0%
!! GCCTTGTTTGTTATTCCAGA+CGG + tig0026181:65726-65745 None:intergenic 40.0%
TTAACTTCAACCCAAATCGG+AGG - tig0026181:65780-65799 Msa1427300:CDS 40.0%
TATCCACAAATGCTCACTAG+GGG + tig0026181:66085-66104 None:intergenic 40.0%
CTATCCACAAATGCTCACTA+GGG + tig0026181:66086-66105 None:intergenic 40.0%
TCTATCCACAAATGCTCACT+AGG + tig0026181:66087-66106 None:intergenic 40.0%
GTGGATAGAAATTGGAGATG+TGG - tig0026181:66098-66117 Msa1427300:intron 40.0%
ATAGAAATTGGAGATGTGGC+AGG - tig0026181:66102-66121 Msa1427300:intron 40.0%
TATATATTCACCCTGCCATG+AGG - tig0026181:66191-66210 Msa1427300:intron 40.0%
TTCTGTTTGCAGCCTAAACT+TGG - tig0026181:66246-66265 Msa1427300:intron 40.0%
GTGGACATCTATTCAGAGAA+AGG + tig0026181:66285-66304 None:intergenic 40.0%
AGCAACATGAATTGCACAAG+TGG + tig0026181:66304-66323 None:intergenic 40.0%
! TTCAGGAGTGGATTTATCAC+AGG - tig0026181:66738-66757 Msa1427300:CDS 40.0%
TTGGAAACACACATTGGCAT+TGG + tig0026181:66820-66839 None:intergenic 40.0%
AATGCCAATGTGTGTTTCCA+AGG - tig0026181:66819-66838 Msa1427300:CDS 40.0%
AAGGATCTTCATCATCCTAG+GGG + tig0026181:66964-66983 None:intergenic 40.0%
GAAGGATCTTCATCATCCTA+GGG + tig0026181:66965-66984 None:intergenic 40.0%
AGAAGGATCTTCATCATCCT+AGG + tig0026181:66966-66985 None:intergenic 40.0%
TGCTGGATTATTATCCTGCA+TGG - tig0026181:67174-67193 Msa1427300:intron 40.0%
!! CTTAGTTTGTTGGGATCCTT+AGG - tig0026181:67222-67241 Msa1427300:intron 40.0%
! TCCCTCTTTTGATCACTGTG+TGG + tig0026181:64955-64974 None:intergenic 45.0%
AGCCAGAAAAACTGATGCCA+TGG - tig0026181:65150-65169 Msa1427300:three_prime_UTR 45.0%
GCCAGAAAAACTGATGCCAT+GGG - tig0026181:65151-65170 Msa1427300:three_prime_UTR 45.0%
! ACCCATGGCATCAGTTTTTC+TGG + tig0026181:65155-65174 None:intergenic 45.0%
TTTCAATCTCGTGGCGCAAA+TGG - tig0026181:65558-65577 Msa1427300:intron 45.0%
GCAAACAGCAATGTTGGAGA+TGG + tig0026181:65594-65613 None:intergenic 45.0%
! ATCTGGCTTGAGCACAACAA+CGG - tig0026181:65680-65699 Msa1427300:CDS 45.0%
GGTTGGGAAGAAATAGGACT+TGG + tig0026181:65753-65772 None:intergenic 45.0%
TCAACCCAAATCGGAGGTTT+AGG - tig0026181:65786-65805 Msa1427300:intron 45.0%
TCCACCTAAACCTCCGATTT+GGG + tig0026181:65793-65812 None:intergenic 45.0%
GAGAAAGAGCTCACCATTGA+AGG - tig0026181:65918-65937 Msa1427300:intron 45.0%
AAAGAGCTCACCATTGAAGG+AGG - tig0026181:65921-65940 Msa1427300:intron 45.0%
GGATCAACCTAGCATGTTGT+TGG - tig0026181:66505-66524 Msa1427300:intron 45.0%
GCTATACTTGGACAGGTTGT+TGG - tig0026181:66690-66709 Msa1427300:CDS 45.0%
!! CACATTGGCATTGGTATGTC+TGG + tig0026181:66811-66830 None:intergenic 45.0%
! TCTGTCGATCATTTTCCCCT+AGG - tig0026181:66946-66965 Msa1427300:intron 45.0%
AATCAAAGGAAGATGCGCTC+TGG - tig0026181:66989-67008 Msa1427300:intron 45.0%
! GGGGTATTTATTTCCTGTGC+TGG - tig0026181:67157-67176 Msa1427300:intron 45.0%
GCAGGATAATAATCCAGCAC+AGG + tig0026181:67173-67192 None:intergenic 45.0%
ACAGTCAATCTCATCCATGC+AGG + tig0026181:67191-67210 None:intergenic 45.0%
ACAGCTTGTATCAGTATGGG+AGG + tig0026181:67307-67326 None:intergenic 45.0%
!!! AATAATTTTTATCAATTATT+TGG - tig0026181:67106-67125 Msa1427300:intron 5.0%
!!! ATAATTTTTATCAATTATTT+GGG - tig0026181:67107-67126 Msa1427300:intron 5.0%
!!! AACTTTTTAAAATTAATTTT+TGG - tig0026181:67132-67151 Msa1427300:intron 5.0%
! CTGGCTCGTTATTCCAACCT+TGG + tig0026181:65136-65155 None:intergenic 50.0%
AATCTCGTGGCGCAAATGGT+GGG - tig0026181:65562-65581 Msa1427300:intron 50.0%
ACCATCTCCCCAACAACTCT+TGG - tig0026181:65639-65658 Msa1427300:intron 50.0%
CCATCTCCCCAACAACTCTT+GGG - tig0026181:65640-65659 Msa1427300:intron 50.0%
CATCTCCCCAACAACTCTTG+GGG - tig0026181:65641-65660 Msa1427300:intron 50.0%
ATCTCCCCAACAACTCTTGG+GGG - tig0026181:65642-65661 Msa1427300:intron 50.0%
GATACCCCCAAGAGTTGTTG+GGG + tig0026181:65649-65668 None:intergenic 50.0%
GGATACCCCCAAGAGTTGTT+GGG + tig0026181:65650-65669 None:intergenic 50.0%
TGGATACCCCCAAGAGTTGT+TGG + tig0026181:65651-65670 None:intergenic 50.0%
ACCCAAATCGGAGGTTTAGG+TGG - tig0026181:65789-65808 Msa1427300:intron 50.0%
CTCCACCTAAACCTCCGATT+TGG + tig0026181:65794-65813 None:intergenic 50.0%
GCTAATGGCACCTCCTTCAA+TGG + tig0026181:65934-65953 None:intergenic 50.0%
CATCCCCTAGTGAGCATTTG+TGG - tig0026181:66079-66098 Msa1427300:intron 50.0%
ACACATGCCTTGCAGAGTTC+AGG - tig0026181:66721-66740 Msa1427300:CDS 50.0%
ATCCACTCCTGAACTCTGCA+AGG + tig0026181:66731-66750 None:intergenic 50.0%
! GCATTGGTATGTCTGGTCTG+AGG + tig0026181:66804-66823 None:intergenic 50.0%
! ATTTTGTTGGGGGGAGCCTA+AGG + tig0026181:67241-67260 None:intergenic 50.0%
! GCTATCACAATGAGGGTGTG+AGG + tig0026181:67466-67485 None:intergenic 50.0%
CAATCTCGTGGCGCAAATGG+TGG - tig0026181:65561-65580 Msa1427300:intron 55.0%
CCCAAGAGTTGTTGGGGAGA+TGG + tig0026181:65643-65662 None:intergenic 55.0%
GGAGATGTGGCAGGTGATTG+TGG - tig0026181:66111-66130 Msa1427300:intron 55.0%
TGCCTTGCAGAGTTCAGGAG+TGG - tig0026181:66726-66745 Msa1427300:CDS 55.0%
!! CGCTCTGGCTCTAGAGTCAC+TGG - tig0026181:67004-67023 Msa1427300:intron 60.0%
Chromosome Type Strat End Strand Name
tig0026181 gene 64934 67551 64934 ID=Msa1427300;Name=Msa1427300
tig0026181 mRNA 64934 67551 64934 ID=Msa1427300-mRNA-1;Parent=Msa1427300;Name=Msa1427300-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.6|0.5|1|1|1|6|473|313
tig0026181 exon 64934 65517 64934 ID=Msa1427300-mRNA-1:exon:5366;Parent=Msa1427300-mRNA-1
tig0026181 exon 65645 65802 65645 ID=Msa1427300-mRNA-1:exon:5365;Parent=Msa1427300-mRNA-1
tig0026181 exon 66362 66429 66362 ID=Msa1427300-mRNA-1:exon:5364;Parent=Msa1427300-mRNA-1
tig0026181 exon 66509 66954 66509 ID=Msa1427300-mRNA-1:exon:5363;Parent=Msa1427300-mRNA-1
tig0026181 exon 67313 67408 67313 ID=Msa1427300-mRNA-1:exon:5362;Parent=Msa1427300-mRNA-1
tig0026181 exon 67489 67551 67489 ID=Msa1427300-mRNA-1:exon:5361;Parent=Msa1427300-mRNA-1
tig0026181 CDS 67489 67551 67489 ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181 CDS 67313 67408 67313 ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181 CDS 66509 66954 66509 ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181 CDS 66362 66429 66362 ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181 CDS 65645 65802 65645 ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181 CDS 65407 65517 65407 ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181 three_prime_UTR 64934 65406 64934 ID=Msa1427300-mRNA-1:three_prime_utr;Parent=Msa1427300-mRNA-1
Gene Sequence

>Msa1427300

ATGCTACGAGAGCTTATTCCACACAGTGATCAAAAGAGGGACAAAGCATCCTTTTTGTTAGAGGTCATTGAGTACATTCACTTTTTACAAGAGAAGGTTCATAAGTATGAAGGTTCATTCCAAGGTTGGAATAACGAGCCAGAAAAACTGATGCCATGGAGAAATAATGACAGGCCAGTAGAAAGCTTTCAATCTCGTGGCGCAAATGGTGGGTCTAATCCATCTCCAACATTGCTGTTTGCTTCAAAGGTTGACGAGAAAAACATCACCATCTCCCCAACAACTCTTGGGGGTATCCATAATGTAGAATCTGGCTTGAGCACAACAACGGCCATGAAGACAATAGATCATCCGTCTGGAATAACAAACAAGGCTTTTCCAAGTCCTATTTCTTCCCAACCAAATTTCTTAACTTCAACCCAAATCGGAGGTTTAGGTGGAGCAGTGTCTCAGCTCAGACGTAGATTATCATCTGATGCAGAGAACACCAATTATCAACCTTCAGTAGAATCTCAGACTATGACTTCAACCAGTGAAAAGCTGAAAGAGAAAGAGCTCACCATTGAAGGAGGTGCCATTAGCATATCAAGTGTGTACTCACAAGGTTGTGTTGGACATGATATGGATTCATCCCCTAGTGAGCATTTGTGGATAGAAATTGGAGATGTGGCAGTAAATGTGCTATACTTGGACAGGTTGTTGGATACACTTACACATGCCTTGCAGAGTTCAGGAGTGGATTTATCACAGGCTAGCATTTCGGTGCAAATTGAACTTGGTAAACAAGCAAGCCTCAGACCAGACATACCAATGCCAATGTGTGTTTCCAAGGATGATGAAGATCCTTCTAAGAATCAAAGGAAGATGCGCTCTGGCTCTAGAGTCACTGGCTCTGAGAAATCTGATCAAGCACTAAAGAAGCTGAAAATGTGCAGAAGTTAA
Protein sequence

>Msa1427300

MLRELIPHSDQKRDKASFLLEVIEYIHFLQEKVHKYEGSFQGWNNEPEKLMPWRNNDRPVESFQSRGANGGSNPSPTLLFASKVDEKNITISPTTLGGIHNVESGLSTTTAMKTIDHPSGITNKAFPSPISSQPNFLTSTQIGGLGGAVSQLRRRLSSDAENTNYQPSVESQTMTSTSEKLKEKELTIEGGAISISSVYSQGCVGHDMDSSPSEHLWIEIGDVAVNVLYLDRLLDTLTHALQSSGVDLSQASISVQIELGKQASLRPDIPMPMCVSKDDEDPSKNQRKMRSGSRVTGSEKSDQALKKLKMCRS*