AlfalfaGEDB | Msa1427300

M. sativa cultivar zhongmu-4 / Msa1427300

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1427300	KEH32062.1	86.901	313	11	1	1	313	156	438	0.0	543

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1427300	sp\|Q9CAA4\|BIM2_ARATH	37.313	268	102	10	1	264	67	272	1.07e-36	136

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1427300	A0A072UQV4	86.901	313	11	1	1	313	156	438	0.0	543

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TFs/TRs:

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Gene ID	Type	Classification
Msa1427300	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1427300	MtrunA17_Chr4g0061791	86.901	313	11	1	1	313	288	570	0.0	543
Msa1427300	MtrunA17_Chr5g0420701	37.931	319	136	8	1	309	66	332	7.84e-55	181
Msa1427300	MtrunA17_Chr7g0220371	35.401	274	120	9	2	264	73	300	7.36e-31	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1427300	AT5G08130.3	39.773	264	92	8	1	264	178	374	3.12e-39	142
Msa1427300	AT5G08130.1	39.394	264	94	8	1	264	178	375	1.29e-38	141
Msa1427300	AT5G08130.7	40.152	264	91	8	1	264	298	494	3.99e-38	142
Msa1427300	AT5G08130.5	39.773	264	92	8	1	264	302	498	4.05e-38	142
Msa1427300	AT1G69010.1	37.313	268	102	10	1	264	67	272	1.09e-37	136
Msa1427300	AT5G08130.2	39.773	264	93	8	1	264	298	495	1.30e-37	140
Msa1427300	AT5G08130.4	39.773	264	93	8	1	264	298	495	1.30e-37	140
Msa1427300	AT5G08130.6	39.394	264	94	8	1	264	298	495	1.39e-37	140
Msa1427300	AT5G08130.8	39.394	264	94	8	1	264	302	499	1.43e-37	140
Msa1427300	AT5G38860.1	32.197	264	109	7	2	262	56	252	1.88e-25	103

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Find 96 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGCTTGTATCAGTAT+GGG	0.573845	tig0026181:+65156	None:intergenic
AAAGAGCTCACCATTGAAGG+AGG	0.629369	tig0026181:-66542	Msa1427300:CDS
AAATTTGGTTGGGAAGAAAT+AGG	0.321463	tig0026181:+66707	None:intergenic
AACATTGCTGTTTGCTTCAA+AGG	0.352246	tig0026181:-66864	Msa1427300:CDS
AAGACAATAGATCATCCGTC+TGG	0.528087	tig0026181:-66755	Msa1427300:CDS
AAGATCCTTCTAAGAATCAA+AGG	0.539351	tig0026181:-65488	Msa1427300:CDS
AAGTATGAAGGTTCATTCCA+AGG	0.551145	tig0026181:-67347	Msa1427300:CDS
AAGTTGTGTTGGACATGATA+TGG	0.317695	tig0026181:-66410	Msa1427300:CDS
AATCAAAGGAAGATGCGCTC+TGG	0.532036	tig0026181:-65474	Msa1427300:CDS
AATCTCGTGGCGCAAATGGT+GGG	0.551190	tig0026181:-66901	Msa1427300:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCTTTCACAGCTTAAGAT+AGG	+	tig0026181:65192-65211	None:intergenic	30.0%
TTTCTCATAAAATGATCAGG+AGG	+	tig0026181:65263-65282	None:intergenic	30.0%
ACGTTTCTCATAAAATGATC+AGG	+	tig0026181:65266-65285	None:intergenic	30.0%
CAATCAAAACTATTAGGTCT+AGG	+	tig0026181:65307-65326	None:intergenic	30.0%
ACCAGACAATCAAAACTATT+AGG	+	tig0026181:65313-65332	None:intergenic	30.0%
ATCACATAGCCTATTGTTAA+TGG	-	tig0026181:65448-65467	Msa1427300:CDS	30.0%
AAATTTGGTTGGGAAGAAAT+AGG	+	tig0026181:65759-65778	None:intergenic	30.0%
TTCTTAACTTCAACCCAAAT+CGG	-	tig0026181:65777-65796	Msa1427300:CDS	30.0%
ATTGAATTGTTAGATGACTG+TGG	+	tig0026181:66151-66170	None:intergenic	30.0%
TGAATTGTTTGAATGGAACA+GGG	+	tig0026181:66443-66462	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0026181	gene	64934	67551	64934	ID=Msa1427300;Name=Msa1427300
tig0026181	mRNA	64934	67551	64934	ID=Msa1427300-mRNA-1;Parent=Msa1427300;Name=Msa1427300-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0.6\|0.5\|1\|1\|1\|6\|473\|313
tig0026181	exon	64934	65517	64934	ID=Msa1427300-mRNA-1:exon:5366;Parent=Msa1427300-mRNA-1
tig0026181	exon	65645	65802	65645	ID=Msa1427300-mRNA-1:exon:5365;Parent=Msa1427300-mRNA-1
tig0026181	exon	66362	66429	66362	ID=Msa1427300-mRNA-1:exon:5364;Parent=Msa1427300-mRNA-1
tig0026181	exon	66509	66954	66509	ID=Msa1427300-mRNA-1:exon:5363;Parent=Msa1427300-mRNA-1
tig0026181	exon	67313	67408	67313	ID=Msa1427300-mRNA-1:exon:5362;Parent=Msa1427300-mRNA-1
tig0026181	exon	67489	67551	67489	ID=Msa1427300-mRNA-1:exon:5361;Parent=Msa1427300-mRNA-1
tig0026181	CDS	67489	67551	67489	ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181	CDS	67313	67408	67313	ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181	CDS	66509	66954	66509	ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181	CDS	66362	66429	66362	ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181	CDS	65645	65802	65645	ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181	CDS	65407	65517	65407	ID=Msa1427300-mRNA-1:cds;Parent=Msa1427300-mRNA-1
tig0026181	three_prime_UTR	64934	65406	64934	ID=Msa1427300-mRNA-1:three_prime_utr;Parent=Msa1427300-mRNA-1

Gene Sequence

>Msa1427300

ATGCTACGAGAGCTTATTCCACACAGTGATCAAAAGAGGGACAAAGCATCCTTTTTGTTAGAGGTCATTGAGTACATTCACTTTTTACAAGAGAAGGTTCATAAGTATGAAGGTTCATTCCAAGGTTGGAATAACGAGCCAGAAAAACTGATGCCATGGAGAAATAATGACAGGCCAGTAGAAAGCTTTCAATCTCGTGGCGCAAATGGTGGGTCTAATCCATCTCCAACATTGCTGTTTGCTTCAAAGGTTGACGAGAAAAACATCACCATCTCCCCAACAACTCTTGGGGGTATCCATAATGTAGAATCTGGCTTGAGCACAACAACGGCCATGAAGACAATAGATCATCCGTCTGGAATAACAAACAAGGCTTTTCCAAGTCCTATTTCTTCCCAACCAAATTTCTTAACTTCAACCCAAATCGGAGGTTTAGGTGGAGCAGTGTCTCAGCTCAGACGTAGATTATCATCTGATGCAGAGAACACCAATTATCAACCTTCAGTAGAATCTCAGACTATGACTTCAACCAGTGAAAAGCTGAAAGAGAAAGAGCTCACCATTGAAGGAGGTGCCATTAGCATATCAAGTGTGTACTCACAAGGTTGTGTTGGACATGATATGGATTCATCCCCTAGTGAGCATTTGTGGATAGAAATTGGAGATGTGGCAGTAAATGTGCTATACTTGGACAGGTTGTTGGATACACTTACACATGCCTTGCAGAGTTCAGGAGTGGATTTATCACAGGCTAGCATTTCGGTGCAAATTGAACTTGGTAAACAAGCAAGCCTCAGACCAGACATACCAATGCCAATGTGTGTTTCCAAGGATGATGAAGATCCTTCTAAGAATCAAAGGAAGATGCGCTCTGGCTCTAGAGTCACTGGCTCTGAGAAATCTGATCAAGCACTAAAGAAGCTGAAAATGTGCAGAAGTTAA

Protein sequence

>Msa1427300

MLRELIPHSDQKRDKASFLLEVIEYIHFLQEKVHKYEGSFQGWNNEPEKLMPWRNNDRPVESFQSRGANGGSNPSPTLLFASKVDEKNITISPTTLGGIHNVESGLSTTTAMKTIDHPSGITNKAFPSPISSQPNFLTSTQIGGLGGAVSQLRRRLSSDAENTNYQPSVESQTMTSTSEKLKEKELTIEGGAISISSVYSQGCVGHDMDSSPSEHLWIEIGDVAVNVLYLDRLLDTLTHALQSSGVDLSQASISVQIELGKQASLRPDIPMPMCVSKDDEDPSKNQRKMRSGSRVTGSEKSDQALKKLKMCRS*