AlfalfaGEDB | Msa1428450

M. sativa cultivar zhongmu-4 / Msa1428450

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1428450	XP_039686362.1	92.958	71	5	0	1	71	1	71	4.89e-37	139

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1428450	A0A2Z6MT37	69.014	71	22	0	1	71	1	71	1.19e-24	106

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TFs/TRs:

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Gene ID	Type	Classification
Msa1428450	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1428450	MtrunA17_Chr5g0401121	46.154	65	35	0	7	71	56	120	2.77e-15	66.6
Msa1428450	MtrunA17_Chr6g0458391	42.029	69	40	0	3	71	221	289	2.35e-14	66.6
Msa1428450	MtrunA17_Chr3g0082121	40.541	74	43	1	7	80	43	115	2.52e-13	63.5
Msa1428450	MtrunA17_Chr7g0258861	43.750	64	35	1	6	69	9	71	3.53e-13	62.8
Msa1428450	MtrunA17_Chr1g0203721	37.681	69	43	0	3	71	59	127	9.59e-12	58.9
Msa1428450	MtrunA17_Chr2g0324011	42.188	64	36	1	6	69	9	71	2.13e-11	58.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 21 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACATACTTCCATCTCTTAAA+CGG	0.421200	tig0026358:-9522	Msa1428450:intergenic
AGATTTGTTTGTTCCAAGGA+GGG	0.566275	tig0026358:+9419	Msa1428450:CDS
CAGATTTGTTTGTTCCAAGG+AGG	0.577739	tig0026358:+9418	Msa1428450:CDS
CCCTGATCTGTATCAGCCCT+AGG	0.568201	tig0026358:-9481	Msa1428450:intergenic
CCTAGGGCTGATACAGATCA+GGG	0.657084	tig0026358:+9481	Msa1428450:CDS
CGGCAGATTTGTTTGTTCCA+AGG	0.478536	tig0026358:+9415	Msa1428450:CDS
CTTAATGCGAAACCGAGTGT+TGG	0.607268	tig0026358:+9272	Msa1428450:CDS
CTTGGAACAAACAAATCTGC+CGG	0.541110	tig0026358:-9414	Msa1428450:intergenic
GCCTAGGGCTGATACAGATC+AGG	0.435601	tig0026358:+9480	Msa1428450:CDS
GTAGATGACTTGATTACATC+CGG	0.515405	tig0026358:+9395	Msa1428450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTGAATATGGTAAGAAATG+TGG	+	tig0026358:9335-9354	Msa1428450:CDS	30.0%
TGATTACTTGAACAAACGAA+AGG	+	tig0026358:9373-9392	Msa1428450:CDS	30.0%
TCAAATTCCAAACCAACACT+CGG	-	tig0026358:9287-9306	Msa1428450:intergenic	35.0%
GTAGATGACTTGATTACATC+CGG	+	tig0026358:9395-9414	Msa1428450:CDS	35.0%
TGAAAGACAAAAGATCCTCT+TGG	+	tig0026358:9450-9469	Msa1428450:CDS	35.0%
CTTGGAACAAACAAATCTGC+CGG	-	tig0026358:9417-9436	Msa1428450:intergenic	40.0%
TCCTCTTGGTTAAAAAGCCT+AGG	+	tig0026358:9464-9483	Msa1428450:CDS	40.0%
CCTCTTGGTTAAAAAGCCTA+GGG	+	tig0026358:9465-9484	Msa1428450:CDS	40.0%
CTTAATGCGAAACCGAGTGT+TGG	+	tig0026358:9272-9291	Msa1428450:CDS	45.0%
TTTGTTCCAAGGAGGGCAGT+AGG	+	tig0026358:9426-9445	Msa1428450:CDS	50.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0026358	gene	9266	9529	9266	ID=Msa1428450;Name=Msa1428450
tig0026358	mRNA	9266	9529	9266	ID=Msa1428450-mRNA-1;Parent=Msa1428450;Name=Msa1428450-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|87
tig0026358	exon	9266	9529	9266	ID=Msa1428450-mRNA-1:exon:5982;Parent=Msa1428450-mRNA-1
tig0026358	CDS	9266	9529	9266	ID=Msa1428450-mRNA-1:cds;Parent=Msa1428450-mRNA-1

Gene Sequence	>Msa1428450 ATGGCACTTAATGCGAAACCGAGTGTTGGTTTGGAATTTGATAGCGTAGATACGACTTGGCAATTTTGGGTTGAATATGGTAAGAAATGTGGTTTTGGAGTTAGAAATGATTACTTGAACAAACGAAAGGTAGATGACTTGATTACATCCGGCAGATTTGTTTGTTCCAAGGAGGGCAGTAGGTTGAAAGACAAAAGATCCTCTTGGTTAAAAAGCCTAGGGCTGATACAGATCAGGGTGTCCAGTAAATTTCATTCCGTTTAA
Protein sequence	>Msa1428450 MALNAKPSVGLEFDSVDTTWQFWVEYGKKCGFGVRNDYLNKRKVDDLITSGRFVCSKEGSRLKDKRSSWLKSLGLIQIRVSSKFHSV*