Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432150 | XP_024636802.1 | 90.521 | 211 | 17 | 1 | 3 | 213 | 25 | 232 | 4.55e-119 | 348 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432150 | A0A396IJH9 | 90.521 | 211 | 17 | 1 | 3 | 213 | 25 | 232 | 2.17e-119 | 348 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1432150 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432150 | MtrunA17_Chr4g0064011 | 90.521 | 211 | 17 | 1 | 3 | 213 | 25 | 232 | 4.19e-123 | 348 |
| Msa1432150 | MtrunA17_Chr3g0122071 | 49.048 | 210 | 80 | 5 | 3 | 210 | 38 | 222 | 5.26e-50 | 162 |
| Msa1432150 | MtrunA17_Chr6g0474091 | 69.388 | 98 | 29 | 1 | 3 | 100 | 25 | 121 | 4.35e-33 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1432150 | AT2G18490.1 | 48.810 | 84 | 37 | 2 | 102 | 185 | 155 | 232 | 5.87e-21 | 87.8 |
| Msa1432150 | AT2G18490.2 | 48.810 | 84 | 37 | 2 | 102 | 185 | 205 | 282 | 1.22e-20 | 88.2 |
| Msa1432150 | AT5G22990.1 | 53.750 | 80 | 36 | 1 | 97 | 175 | 227 | 306 | 8.30e-20 | 86.3 |
| Msa1432150 | AT2G15740.1 | 59.701 | 67 | 23 | 1 | 101 | 167 | 229 | 291 | 1.72e-19 | 85.1 |
| Msa1432150 | AT5G42640.1 | 51.471 | 68 | 29 | 1 | 95 | 158 | 218 | 285 | 2.18e-18 | 82.0 |
Find 32 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCATCTGATAATAATAAT+AGG | 0.318777 | tig0026958:-20873 | Msa1432150:CDS |
| AATGATGGAACAACTACTTT+TGG | 0.332371 | tig0026958:-20460 | Msa1432150:CDS |
| CAAATGCTTGAAGCTCAGTT+TGG | 0.351149 | tig0026958:-20916 | Msa1432150:CDS |
| AACAATACTGTTGCTACTAA+TGG | 0.363944 | tig0026958:-20739 | Msa1432150:CDS |
| AAGTGGGGTAAAAGTTAAAC+TGG | 0.383067 | tig0026958:-20356 | Msa1432150:CDS |
| CTACTAATGGTGATGAAATC+CGG | 0.385370 | tig0026958:-20726 | Msa1432150:CDS |
| TTACACTTTGGACACGTATA+CGG | 0.389805 | tig0026958:+20616 | None:intergenic |
| CGATTTCAAAAGCTCAATGA+TGG | 0.441823 | tig0026958:-20475 | Msa1432150:CDS |
| AAATGCTTGAAGCTCAGTTT+GGG | 0.444522 | tig0026958:-20915 | Msa1432150:CDS |
| CTATTATTATTATCAGATGA+TGG | 0.447972 | tig0026958:+20874 | None:intergenic |
| GACGAGCCTCCAAGGAGATC+AGG | 0.460583 | tig0026958:+20793 | None:intergenic |
| GCTAGTACTTTGTTACACTT+TGG | 0.466837 | tig0026958:+20604 | None:intergenic |
| CCCCTACTCCTCCTCTAAGT+GGG | 0.481758 | tig0026958:-20372 | Msa1432150:CDS |
| GCTCATCCTGATCTCCTTGG+AGG | 0.493768 | tig0026958:-20799 | Msa1432150:CDS |
| TATGCTCATCCTGATCTCCT+TGG | 0.493878 | tig0026958:-20802 | Msa1432150:CDS |
| AAAGAAGAAATTGTTGATGA+TGG | 0.494936 | tig0026958:-20673 | Msa1432150:CDS |
| GCCCCTACTCCTCCTCTAAG+TGG | 0.499796 | tig0026958:-20373 | Msa1432150:CDS |
| ACAGTATTGTTTACCTCAGA+TGG | 0.506108 | tig0026958:+20751 | None:intergenic |
| CCCCACTTAGAGGAGGAGTA+GGG | 0.515661 | tig0026958:+20371 | None:intergenic |
| AATATAAATTAATTATCAGC+AGG | 0.519786 | tig0026958:+20331 | None:intergenic |
| TGTTGGTATTATTAGAAGCC+CGG | 0.523272 | tig0026958:+20707 | None:intergenic |
| GACTGATCAACAGAAGCAAT+AGG | 0.525212 | tig0026958:+20436 | None:intergenic |
| TACTAATGGTGATGAAATCC+GGG | 0.531216 | tig0026958:-20725 | Msa1432150:CDS |
| AAAGTGAAGACGAGCCTCCA+AGG | 0.567472 | tig0026958:+20785 | None:intergenic |
| ACCCCACTTAGAGGAGGAGT+AGG | 0.570382 | tig0026958:+20370 | None:intergenic |
| CTAATAATACCAACATGAAG+CGG | 0.574702 | tig0026958:-20699 | Msa1432150:CDS |
| CCCTACTCCTCCTCTAAGTG+GGG | 0.599231 | tig0026958:-20371 | Msa1432150:CDS |
| GAGATCAGGATGAGCATAAG+CGG | 0.608512 | tig0026958:+20807 | None:intergenic |
| TGTCATCATCAACAACAAGA+TGG | 0.630742 | tig0026958:-20845 | Msa1432150:CDS |
| TTCACAACAACCGCCATCTG+AGG | 0.636470 | tig0026958:-20764 | Msa1432150:CDS |
| CCCACTTAGAGGAGGAGTAG+GGG | 0.654382 | tig0026958:+20372 | None:intergenic |
| GTATTGTTTACCTCAGATGG+CGG | 0.675983 | tig0026958:+20754 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTATTATTATTATCAGATGA+TGG | + | tig0026958:20426-20445 | None:intergenic | 20.0% |
| !! | CATCATCTGATAATAATAAT+AGG | - | tig0026958:20424-20443 | Msa1432150:CDS | 20.0% |
| !! | AAAGAAGAAATTGTTGATGA+TGG | - | tig0026958:20624-20643 | Msa1432150:CDS | 25.0% |
| AACAATACTGTTGCTACTAA+TGG | - | tig0026958:20558-20577 | Msa1432150:CDS | 30.0% | |
| CTAATAATACCAACATGAAG+CGG | - | tig0026958:20598-20617 | Msa1432150:CDS | 30.0% | |
| AGTCTACCACACAATAAAAA+TGG | - | tig0026958:20657-20676 | Msa1432150:CDS | 30.0% | |
| GAAAAAAGAGATACAAGTCT+AGG | - | tig0026958:20778-20797 | Msa1432150:CDS | 30.0% | |
| !! | AATGATGGAACAACTACTTT+TGG | - | tig0026958:20837-20856 | Msa1432150:CDS | 30.0% |
| AAATGCTTGAAGCTCAGTTT+GGG | - | tig0026958:20382-20401 | Msa1432150:CDS | 35.0% | |
| TGTCATCATCAACAACAAGA+TGG | - | tig0026958:20452-20471 | Msa1432150:CDS | 35.0% | |
| ACAGTATTGTTTACCTCAGA+TGG | + | tig0026958:20549-20568 | None:intergenic | 35.0% | |
| ! | CTACTAATGGTGATGAAATC+CGG | - | tig0026958:20571-20590 | Msa1432150:CDS | 35.0% |
| ! | TACTAATGGTGATGAAATCC+GGG | - | tig0026958:20572-20591 | Msa1432150:CDS | 35.0% |
| TGTTGGTATTATTAGAAGCC+CGG | + | tig0026958:20593-20612 | None:intergenic | 35.0% | |
| !!! | TTCTTTTTTCCGCTTCATGT+TGG | + | tig0026958:20610-20629 | None:intergenic | 35.0% |
| ! | TTTTATTGTGTGGTAGACTG+TGG | + | tig0026958:20656-20675 | None:intergenic | 35.0% |
| !! | TACGGTCCATTTTTATTGTG+TGG | + | tig0026958:20666-20685 | None:intergenic | 35.0% |
| TTACACTTTGGACACGTATA+CGG | + | tig0026958:20684-20703 | None:intergenic | 35.0% | |
| GCTAGTACTTTGTTACACTT+TGG | + | tig0026958:20696-20715 | None:intergenic | 35.0% | |
| !!! | AGCTTTTGAAATCGAAGATC+TGG | + | tig0026958:20816-20835 | None:intergenic | 35.0% |
| CGATTTCAAAAGCTCAATGA+TGG | - | tig0026958:20822-20841 | Msa1432150:CDS | 35.0% | |
| ! | TTAACTTTTACCCCACTTAG+AGG | + | tig0026958:20939-20958 | None:intergenic | 35.0% |
| AAGTGGGGTAAAAGTTAAAC+TGG | - | tig0026958:20941-20960 | Msa1432150:CDS | 35.0% | |
| CAAATGCTTGAAGCTCAGTT+TGG | - | tig0026958:20381-20400 | Msa1432150:CDS | 40.0% | |
| GTATTGTTTACCTCAGATGG+CGG | + | tig0026958:20546-20565 | None:intergenic | 40.0% | |
| GACTGATCAACAGAAGCAAT+AGG | + | tig0026958:20864-20883 | None:intergenic | 40.0% | |
| ! | ATTTTCGGTAGTCATGCTCC+AGG | - | tig0026958:20342-20361 | Msa1432150:CDS | 45.0% |
| !! | TGCATCGCTTTTATGATGCC+TGG | + | tig0026958:20363-20382 | None:intergenic | 45.0% |
| GAGATCAGGATGAGCATAAG+CGG | + | tig0026958:20493-20512 | None:intergenic | 45.0% | |
| TATGCTCATCCTGATCTCCT+TGG | - | tig0026958:20495-20514 | Msa1432150:CDS | 45.0% | |
| ! | ACTTTTACCCCACTTAGAGG+AGG | + | tig0026958:20936-20955 | None:intergenic | 45.0% |
| AAAGTGAAGACGAGCCTCCA+AGG | + | tig0026958:20515-20534 | None:intergenic | 50.0% | |
| TTCACAACAACCGCCATCTG+AGG | - | tig0026958:20533-20552 | Msa1432150:CDS | 50.0% | |
| GCTCATCCTGATCTCCTTGG+AGG | - | tig0026958:20498-20517 | Msa1432150:CDS | 55.0% | |
| CCCACTTAGAGGAGGAGTAG+GGG | + | tig0026958:20928-20947 | None:intergenic | 55.0% | |
| CCCCTACTCCTCCTCTAAGT+GGG | - | tig0026958:20925-20944 | Msa1432150:CDS | 55.0% | |
| CCCCACTTAGAGGAGGAGTA+GGG | + | tig0026958:20929-20948 | None:intergenic | 55.0% | |
| CCCTACTCCTCCTCTAAGTG+GGG | - | tig0026958:20926-20945 | Msa1432150:CDS | 55.0% | |
| ACCCCACTTAGAGGAGGAGT+AGG | + | tig0026958:20930-20949 | None:intergenic | 55.0% | |
| GACGAGCCTCCAAGGAGATC+AGG | + | tig0026958:20507-20526 | None:intergenic | 60.0% | |
| GCCCCTACTCCTCCTCTAAG+TGG | - | tig0026958:20924-20943 | Msa1432150:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0026958 | gene | 20339 | 20980 | 20339 | ID=Msa1432150;Name=Msa1432150 |
| tig0026958 | mRNA | 20339 | 20980 | 20339 | ID=Msa1432150-mRNA-1;Parent=Msa1432150;Name=Msa1432150-mRNA-1;_AED=0.12;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|213 |
| tig0026958 | exon | 20339 | 20980 | 20339 | ID=Msa1432150-mRNA-1:exon:7926;Parent=Msa1432150-mRNA-1 |
| tig0026958 | CDS | 20339 | 20980 | 20339 | ID=Msa1432150-mRNA-1:cds;Parent=Msa1432150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
