AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1433030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433030 MtrunA17_Chr2g0320691 99.291 141 1 0 1 141 1 141 9.23e-100 299
Msa1433030 MtrunA17_Chr2g0303541 50.735 136 66 1 6 141 9 143 8.59e-45 154
Msa1433030 MtrunA17_Chr4g0050021 46.377 138 72 2 6 143 27 162 3.91e-41 144
Msa1433030 MtrunA17_Chr2g0279481 46.763 139 70 2 6 143 27 162 2.08e-38 137
Msa1433030 MtrunA17_Chr2g0285511 40.876 137 80 1 5 141 8 143 3.69e-32 119
Msa1433030 MtrunA17_Chr1g0181181 42.424 132 75 1 10 141 13 143 2.22e-31 117
Msa1433030 MtrunA17_Chr8g0374011 40.000 145 69 2 13 139 10 154 6.38e-31 116
Msa1433030 MtrunA17_Chr8g0367891 46.087 115 61 1 26 140 29 142 2.12e-30 114
Msa1433030 MtrunA17_Chr5g0401341 48.305 118 58 1 23 140 27 141 2.90e-30 114
Msa1433030 MtrunA17_Chr1g0150191 46.667 120 62 1 21 140 25 142 4.05e-30 114
Msa1433030 MtrunA17_Chr8g0382231 38.760 129 78 1 15 143 44 171 1.11e-29 112
Msa1433030 MtrunA17_Chr4g0076521 44.538 119 64 1 21 139 24 140 2.29e-29 111
Msa1433030 MtrunA17_Chr1g0207461 42.857 133 72 2 10 140 13 143 3.06e-27 105
Msa1433030 MtrunA17_Chr7g0268781 40.336 119 69 1 21 139 34 150 5.04e-27 105
Msa1433030 MtrunA17_Chr2g0290741 40.336 119 69 1 21 139 24 140 6.08e-27 104
Msa1433030 MtrunA17_Chr4g0010091 40.171 117 68 1 23 139 34 148 6.60e-27 104
Msa1433030 MtrunA17_Chr5g0401451 38.983 118 68 2 17 133 34 148 7.24e-27 104
Msa1433030 MtrunA17_Chr1g0195911 42.017 119 67 1 21 139 34 150 8.63e-27 104
Msa1433030 MtrunA17_Chr4g0053841 35.772 123 79 0 17 139 7 129 1.01e-26 104
Msa1433030 MtrunA17_Chr1g0191711 35.433 127 80 1 13 137 48 174 1.12e-26 103
Msa1433030 MtrunA17_Chr7g0245201 44.068 118 61 3 18 134 58 171 1.79e-26 103
Msa1433030 MtrunA17_Chr5g0432861 40.157 127 72 2 4 129 6 129 4.20e-24 96.7
Msa1433030 MtrunA17_Chr1g0199631 42.718 103 57 2 41 143 8 108 6.46e-23 93.6
Msa1433030 MtrunA17_Chr5g0412591 44.086 93 52 0 21 113 24 116 8.37e-22 90.1
Msa1433030 MtrunA17_Chr3g0108331 35.938 128 80 1 2 129 3 128 1.02e-21 90.1
Msa1433030 MtrunA17_Chr1g0206621 32.743 113 76 0 27 139 72 184 6.70e-20 82.4
Msa1433030 MtrunA17_Chr4g0037661 32.773 119 77 3 26 141 6 124 1.18e-15 72.8
Msa1433030 MtrunA17_Chr1g0163311 31.193 109 68 2 36 143 17 119 4.16e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433030 AT3G44820.1 64.789 142 49 1 1 141 1 142 1.67e-62 202
Msa1433030 AT1G30440.1 48.529 136 70 0 6 141 9 144 1.13e-42 149
Msa1433030 AT5G64330.2 47.826 138 70 1 6 143 35 170 8.71e-41 144
Msa1433030 AT5G64330.1 47.826 138 70 1 6 143 35 170 1.30e-40 144
Msa1433030 AT5G03250.1 48.529 136 69 1 6 141 9 143 1.07e-38 137
Msa1433030 AT5G13600.1 45.455 132 69 1 10 141 13 141 3.67e-36 130
Msa1433030 AT1G67900.5 45.000 120 61 1 21 140 24 138 2.83e-31 117
Msa1433030 AT1G67900.1 45.000 120 61 1 21 140 24 138 2.83e-31 117
Msa1433030 AT1G67900.3 45.000 120 61 1 21 140 24 138 2.83e-31 117
Msa1433030 AT1G67900.6 45.000 120 61 1 21 140 24 138 2.83e-31 117
Msa1433030 AT1G67900.2 45.000 120 61 1 21 140 24 138 2.83e-31 117
Msa1433030 AT1G67900.4 45.000 120 61 1 21 140 24 138 2.83e-31 117
Msa1433030 AT5G10250.2 38.636 132 80 1 8 139 35 165 8.66e-31 116
Msa1433030 AT5G10250.1 38.636 132 80 1 8 139 35 165 8.83e-31 116
Msa1433030 AT4G31820.1 44.715 123 66 1 18 140 22 142 2.00e-30 115
Msa1433030 AT5G66560.1 34.682 173 71 2 13 143 9 181 2.90e-30 114
Msa1433030 AT3G08660.1 43.902 123 64 2 18 140 31 148 4.73e-30 114
Msa1433030 AT3G08570.1 42.623 122 68 1 18 139 31 150 5.48e-30 114
Msa1433030 AT5G66560.2 34.682 173 71 2 13 143 9 181 6.58e-30 112
Msa1433030 AT5G48800.1 43.590 117 64 1 23 139 41 155 7.10e-30 113
Msa1433030 AT5G67440.3 46.721 122 58 2 23 140 27 145 7.24e-30 113
Msa1433030 AT5G67440.1 46.721 122 58 2 23 140 27 145 7.24e-30 113
Msa1433030 AT3G08660.2 42.975 121 66 1 21 141 32 149 8.95e-30 112
Msa1433030 AT3G50840.1 42.636 129 69 2 17 143 10 135 5.81e-29 110
Msa1433030 AT2G14820.2 39.860 143 79 3 1 140 4 142 7.66e-28 107
Msa1433030 AT2G14820.1 39.860 143 79 3 1 140 4 142 7.66e-28 107
Msa1433030 AT4G37590.1 42.424 132 67 2 18 140 22 153 8.15e-28 107
Msa1433030 AT5G47800.3 40.678 118 68 1 24 141 27 142 1.43e-27 107
Msa1433030 AT5G47800.1 40.678 118 68 1 24 141 27 142 1.43e-27 107
Msa1433030 AT5G47800.4 40.678 118 68 1 24 141 27 142 1.43e-27 107
Msa1433030 AT5G47800.2 40.678 118 68 1 24 141 140 255 1.90e-27 106
Msa1433030 AT5G67385.1 36.585 123 77 1 17 139 19 140 5.08e-27 105
Msa1433030 AT5G64330.3 45.370 108 57 1 36 143 6 111 1.09e-26 104
Msa1433030 AT1G03010.3 42.063 126 66 2 7 132 21 139 1.95e-25 100
Msa1433030 AT2G23050.1 45.536 112 58 1 33 141 37 148 1.99e-25 100
Msa1433030 AT1G03010.2 43.750 112 58 1 21 132 7 113 2.10e-25 100
Msa1433030 AT1G03010.1 43.750 112 58 1 21 132 34 140 2.15e-25 100
Msa1433030 AT2G30520.1 35.333 150 88 2 1 143 1 148 2.21e-25 100
Msa1433030 AT2G47860.4 40.336 119 66 2 21 139 35 148 1.48e-24 97.8
Msa1433030 AT2G47860.1 40.336 119 66 2 21 139 35 148 2.41e-24 97.8
Msa1433030 AT5G67440.2 45.794 107 51 2 38 140 5 108 7.81e-24 96.3
Msa1433030 AT3G26490.2 38.017 121 71 2 21 137 24 144 4.38e-23 94.0
Msa1433030 AT3G26490.1 38.017 121 71 2 21 137 24 144 5.03e-23 94.0
Msa1433030 AT3G26490.3 38.017 121 71 2 21 137 24 144 5.03e-23 94.0
Msa1433030 AT3G26490.4 38.017 121 71 2 21 137 24 144 5.03e-23 94.0
Msa1433030 AT3G49970.1 41.346 104 58 2 31 134 2 102 5.35e-22 91.3
Msa1433030 AT3G49970.2 41.346 104 58 2 31 134 2 102 5.35e-22 91.3
Msa1433030 AT5G66560.3 32.895 152 60 2 34 143 1 152 2.42e-21 89.4
Msa1433030 AT4G31820.2 49.367 79 40 0 62 140 11 89 7.85e-20 85.1
Msa1433030 AT2G47860.6 33.803 142 66 3 21 139 35 171 7.88e-20 84.7
Msa1433030 AT2G47860.3 33.803 142 66 3 21 139 35 171 9.17e-20 84.7
Msa1433030 AT3G08660.3 41.304 92 48 1 49 140 3 88 1.75e-19 84.0
Msa1433030 AT5G66560.4 46.753 77 41 0 67 143 66 142 2.42e-19 83.6
Msa1433030 AT5G48130.1 32.283 127 80 2 17 143 33 153 6.54e-17 76.6
Msa1433030 AT2G30520.2 37.624 101 61 1 43 143 2 100 6.95e-17 76.6
Msa1433030 AT2G30520.3 37.624 101 61 1 43 143 2 100 6.95e-17 76.6
Msa1433030 AT5G67385.4 33.333 96 63 1 44 139 1 95 7.55e-17 76.3
Msa1433030 AT5G67385.5 33.333 96 63 1 44 139 1 95 7.55e-17 76.3
Msa1433030 AT5G67385.2 33.333 96 63 1 44 139 1 95 7.55e-17 76.3
Msa1433030 AT5G67385.3 33.333 96 63 1 44 139 1 95 7.55e-17 76.3
Msa1433030 AT3G50840.2 45.455 77 42 0 67 143 23 99 1.39e-16 75.5
Msa1433030 AT3G50840.3 45.455 77 42 0 67 143 23 99 1.39e-16 75.5
Msa1433030 AT5G17580.2 34.454 119 75 2 25 143 22 137 2.68e-15 72.0
Msa1433030 AT5G17580.1 34.454 119 75 2 25 143 7 122 3.20e-15 71.6
Msa1433030 AT3G03510.1 41.791 67 39 0 75 141 4 70 1.40e-14 69.7
Msa1433030 AT3G19850.1 35.443 79 51 0 65 143 50 128 2.16e-13 66.6

Find 44 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCACCTGGGAAGCTTTC+TGG 0.046977 tig0027224:-2009 Msa1433030:CDS
TTTGGTAGGTTTAGTGATTC+TGG 0.090514 tig0027224:-1761 Msa1433030:intron
GCCACAATCAAGAAAGTTTC+AGG 0.210799 tig0027224:+838 None:intergenic
TTAACTGAATTCCGAGAATT+TGG 0.222585 tig0027224:-2078 Msa1433030:five_prime_UTR
CTCCACCTGGGAAGCTTTCT+GGG 0.231623 tig0027224:-2008 Msa1433030:CDS
GTTCTTCTCATAGGTATTCT+TGG 0.237719 tig0027224:+894 None:intergenic
ACGAAACCCGTAACAGAATT+TGG 0.245072 tig0027224:+816 None:intergenic
CAACTGGAAAGATTGCATTT+TGG 0.261760 tig0027224:-665 Msa1433030:CDS
TTATTTCCTTTGCTTCAAAA+TGG 0.324437 tig0027224:-2031 Msa1433030:exon
CTTTCTGGGTTTCATCTTCA+AGG 0.343380 tig0027224:-1994 Msa1433030:CDS
ATAGAATAGGCTCAGAGCTT+TGG 0.372959 tig0027224:+640 None:intergenic
TTGTGGCCAAATTCTGTTAC+GGG 0.375010 tig0027224:-822 Msa1433030:CDS
TTGGTAGGTTTAGTGATTCT+GGG 0.376444 tig0027224:-1760 Msa1433030:intron
CGTATTCATCCGTCATTTCA+AGG 0.390444 tig0027224:+743 None:intergenic
TCCTGAAACTTTCTTGATTG+TGG 0.400615 tig0027224:-839 Msa1433030:CDS
GTGGTGCTGGAAGAATTTCC+TGG 0.413339 tig0027224:-865 Msa1433030:CDS
TGCTTCAAAATGGCTCCACC+TGG 0.443870 tig0027224:-2021 Msa1433030:exon
CTTGGAATCTTCACAGGTAC+GGG 0.453642 tig0027224:+912 None:intergenic
CACTGTCAGGTTCTTCTCAT+AGG 0.456166 tig0027224:+885 None:intergenic
GATAGAGTAACTGAGATATT+CGG 0.456605 tig0027224:-2143 Msa1433030:five_prime_UTR
ATTGTGGCCAAATTCTGTTA+CGG 0.459464 tig0027224:-823 Msa1433030:CDS
GATATTACCGTGTCAATTGA+TGG 0.469085 tig0027224:-1728 Msa1433030:CDS
TTTCCTCTTGTTTCAAGATG+TGG 0.472009 tig0027224:-943 Msa1433030:CDS
ATTCTTCCAGCACCACTGTC+AGG 0.483228 tig0027224:+872 None:intergenic
TTCATCTTCAAGGTCATCAC+TGG 0.484382 tig0027224:-1984 Msa1433030:intron
TGAAACCCAGAAAGCTTCCC+AGG 0.493717 tig0027224:+2003 None:intergenic
TCTTGGAATCTTCACAGGTA+CGG 0.512815 tig0027224:+911 None:intergenic
GAAGAACCTGACAGTGGTGC+TGG 0.515078 tig0027224:-878 Msa1433030:CDS
TGGTGCTGGAAGAATTTCCT+GGG 0.521506 tig0027224:-864 Msa1433030:CDS
GGTATTCTTGGAATCTTCAC+AGG 0.528174 tig0027224:+906 None:intergenic
AAGTGCATACCTTGTGAAGA+TGG 0.532440 tig0027224:+1696 None:intergenic
TTTCCACATCTTGAAACAAG+AGG 0.538173 tig0027224:+940 None:intergenic
ATGACGGATGAATACGAAGA+AGG 0.589120 tig0027224:-736 Msa1433030:CDS
GCTTCAAAATGGCTCCACCT+GGG 0.603801 tig0027224:-2020 Msa1433030:exon
TCTCAGTTACTCTATCATCA+TGG 0.604133 tig0027224:+2150 None:intergenic
ATTGACACGGTAATATCACT+TGG 0.619878 tig0027224:+1734 None:intergenic
GGTGCTGGAAGAATTTCCTG+GGG 0.637441 tig0027224:-863 Msa1433030:CDS
TGCAGAATACCTTGAAATGA+CGG 0.639662 tig0027224:-752 Msa1433030:CDS
TGTCAATTTCCATCTTCACA+AGG 0.664191 tig0027224:-1705 Msa1433030:intron
AACCCAGAAAGCTTCCCAGG+TGG 0.686788 tig0027224:+2006 None:intergenic
ATTGACACCATCAATTGACA+CGG 0.690615 tig0027224:+1721 None:intergenic
GTGCTGGAAGAATTTCCTGG+GGG 0.715345 tig0027224:-862 Msa1433030:CDS
CAAGGTATTCTGCAGCACAG+TGG 0.723725 tig0027224:+761 None:intergenic
CTATGAGAAGAACCTGACAG+TGG 0.738065 tig0027224:-884 Msa1433030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTAAAAAAAAAAA+AGG + tig0027224:670-689 None:intergenic 0.0%
!!! TTTTTTTTTAAAAAAAAAAA+AGG + tig0027224:670-689 None:intergenic 0.0%
!! CTTCTTTATTATTTATTTAT+TGG - tig0027224:923-942 Msa1433030:CDS 10.0%
!! CTTCTTTATTATTTATTTAT+TGG - tig0027224:923-942 Msa1433030:CDS 10.0%
!! ATTTGGTTAAAAAAGTAAAA+AGG - tig0027224:799-818 Msa1433030:CDS 15.0%
!!! ATTTTTTTTTCCTGTTTTTT+TGG - tig0027224:1068-1087 Msa1433030:intron 15.0%
!! TATTCATAATAATTTGATAG+TGG - tig0027224:1224-1243 Msa1433030:intron 15.0%
!! AAATAATCTAACAGTAAAAT+AGG + tig0027224:1356-1375 None:intergenic 15.0%
!!! AAAACTTTTTTACATCAATA+GGG + tig0027224:1387-1406 None:intergenic 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG + tig0027224:1388-1407 None:intergenic 15.0%
!! TATATTGGAGAAAATTTATT+TGG - tig0027224:1715-1734 Msa1433030:CDS 15.0%
!! ATTTGGTTAAAAAAGTAAAA+AGG - tig0027224:799-818 Msa1433030:CDS 15.0%
!!! ATTTTTTTTTCCTGTTTTTT+TGG - tig0027224:1068-1087 Msa1433030:intron 15.0%
!! TATTCATAATAATTTGATAG+TGG - tig0027224:1224-1243 Msa1433030:intron 15.0%
!! AAATAATCTAACAGTAAAAT+AGG + tig0027224:1356-1375 None:intergenic 15.0%
!!! AAAACTTTTTTACATCAATA+GGG + tig0027224:1387-1406 None:intergenic 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG + tig0027224:1388-1407 None:intergenic 15.0%
!! TATATTGGAGAAAATTTATT+TGG - tig0027224:1715-1734 Msa1433030:CDS 15.0%
!!! TACTTTTTTAACCAAATTCT+CGG + tig0027224:796-815 None:intergenic 20.0%
!! AAACAAAATTCCAAAAAAAC+AGG + tig0027224:1081-1100 None:intergenic 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG - tig0027224:1081-1100 Msa1433030:intron 20.0%
!! GAAAAGAAACAATTATGAAA+TGG + tig0027224:1190-1209 None:intergenic 20.0%
!!! TATTATGAATAGAGTATTAG+TGG + tig0027224:1215-1234 None:intergenic 20.0%
!! ACAAAAGAACATAAATGAAT+AGG + tig0027224:1291-1310 None:intergenic 20.0%
!!! ACTAAATGCATTTAAAATGT+TGG + tig0027224:1631-1650 None:intergenic 20.0%
!!! AGGTGAATATTTTGATATAT+TGG - tig0027224:1700-1719 Msa1433030:intron 20.0%
!!! TACTTTTTTAACCAAATTCT+CGG + tig0027224:796-815 None:intergenic 20.0%
!! AAACAAAATTCCAAAAAAAC+AGG + tig0027224:1081-1100 None:intergenic 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG - tig0027224:1081-1100 Msa1433030:intron 20.0%
!! GAAAAGAAACAATTATGAAA+TGG + tig0027224:1190-1209 None:intergenic 20.0%
!!! TATTATGAATAGAGTATTAG+TGG + tig0027224:1215-1234 None:intergenic 20.0%
!! ACAAAAGAACATAAATGAAT+AGG + tig0027224:1291-1310 None:intergenic 20.0%
!!! ACTAAATGCATTTAAAATGT+TGG + tig0027224:1631-1650 None:intergenic 20.0%
!!! AGGTGAATATTTTGATATAT+TGG - tig0027224:1700-1719 Msa1433030:intron 20.0%
! TGGTTAAAAAAGTAAAAAGG+AGG - tig0027224:802-821 Msa1433030:CDS 25.0%
! GGTTAAAAAAGTAAAAAGGA+GGG - tig0027224:803-822 Msa1433030:CDS 25.0%
! TTATTTCCTTTGCTTCAAAA+TGG - tig0027224:829-848 Msa1433030:CDS 25.0%
!!! GAAAATGACATGTTTTTTAG+TGG - tig0027224:991-1010 Msa1433030:intron 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG - tig0027224:1085-1104 Msa1433030:intron 25.0%
!! TTTTGTGAAACATAATTGCA+TGG - tig0027224:1802-1821 Msa1433030:intron 25.0%
! AATTACTTGTAATGTTGTGT+TGG - tig0027224:1864-1883 Msa1433030:intron 25.0%
!!! AGTTGCGCAATATATTTTTA+TGG + tig0027224:2180-2199 None:intergenic 25.0%
! ATAAAAATATATTGCGCAAC+TGG - tig0027224:2179-2198 Msa1433030:five_prime_UTR 25.0%
! TGGTTAAAAAAGTAAAAAGG+AGG - tig0027224:802-821 Msa1433030:CDS 25.0%
! GGTTAAAAAAGTAAAAAGGA+GGG - tig0027224:803-822 Msa1433030:CDS 25.0%
! TTATTTCCTTTGCTTCAAAA+TGG - tig0027224:829-848 Msa1433030:CDS 25.0%
!!! GAAAATGACATGTTTTTTAG+TGG - tig0027224:991-1010 Msa1433030:intron 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG - tig0027224:1085-1104 Msa1433030:intron 25.0%
!! TTTTGTGAAACATAATTGCA+TGG - tig0027224:1802-1821 Msa1433030:intron 25.0%
! AATTACTTGTAATGTTGTGT+TGG - tig0027224:1864-1883 Msa1433030:intron 25.0%
!!! AGTTGCGCAATATATTTTTA+TGG + tig0027224:2180-2199 None:intergenic 25.0%
! ATAAAAATATATTGCGCAAC+TGG - tig0027224:2179-2198 Msa1433030:five_prime_UTR 25.0%
GATAGAGTAACTGAGATATT+CGG - tig0027224:717-736 Msa1433030:CDS 30.0%
TTAACTGAATTCCGAGAATT+TGG - tig0027224:782-801 Msa1433030:CDS 30.0%
!! ATAATTTGATAGTGGAGTAG+TGG - tig0027224:1232-1251 Msa1433030:intron 30.0%
!!! GTTGAGATTAGTTTCTTTTG+TGG - tig0027224:1254-1273 Msa1433030:intron 30.0%
GTAAAATAGGTCACAAAGTA+GGG + tig0027224:1343-1362 None:intergenic 30.0%
AGTAAAATAGGTCACAAAGT+AGG + tig0027224:1344-1363 None:intergenic 30.0%
TATAAAGTCACACGTATGAT+TGG + tig0027224:1430-1449 None:intergenic 30.0%
!! TTTTTGACTCTTTACATCTG+CGG - tig0027224:1474-1493 Msa1433030:intron 30.0%
TGTGATCTCTAGTTAATGAA+GGG + tig0027224:1542-1561 None:intergenic 30.0%
ATGTGATCTCTAGTTAATGA+AGG + tig0027224:1543-1562 None:intergenic 30.0%
GTTATGTGGCTACAATTAAA+AGG - tig0027224:1674-1693 Msa1433030:intron 30.0%
TGGCTACAATTAAAAGGAAA+AGG - tig0027224:1680-1699 Msa1433030:intron 30.0%
GAGAAAATTTATTTGGAAGC+TGG - tig0027224:1722-1741 Msa1433030:CDS 30.0%
TATTTCACCATGCAATTATG+CGG + tig0027224:1776-1795 None:intergenic 30.0%
CATAATTGCATGGTGAAATA+GGG - tig0027224:1776-1795 Msa1433030:intron 30.0%
ATAATTGCATGGTGAAATAG+GGG - tig0027224:1777-1796 Msa1433030:intron 30.0%
GATAGAGTAACTGAGATATT+CGG - tig0027224:717-736 Msa1433030:CDS 30.0%
TTAACTGAATTCCGAGAATT+TGG - tig0027224:782-801 Msa1433030:CDS 30.0%
!! ATAATTTGATAGTGGAGTAG+TGG - tig0027224:1232-1251 Msa1433030:intron 30.0%
!!! GTTGAGATTAGTTTCTTTTG+TGG - tig0027224:1254-1273 Msa1433030:intron 30.0%
GTAAAATAGGTCACAAAGTA+GGG + tig0027224:1343-1362 None:intergenic 30.0%
AGTAAAATAGGTCACAAAGT+AGG + tig0027224:1344-1363 None:intergenic 30.0%
TATAAAGTCACACGTATGAT+TGG + tig0027224:1430-1449 None:intergenic 30.0%
!! TTTTTGACTCTTTACATCTG+CGG - tig0027224:1474-1493 Msa1433030:intron 30.0%
TGTGATCTCTAGTTAATGAA+GGG + tig0027224:1542-1561 None:intergenic 30.0%
ATGTGATCTCTAGTTAATGA+AGG + tig0027224:1543-1562 None:intergenic 30.0%
GTTATGTGGCTACAATTAAA+AGG - tig0027224:1674-1693 Msa1433030:intron 30.0%
TGGCTACAATTAAAAGGAAA+AGG - tig0027224:1680-1699 Msa1433030:intron 30.0%
GAGAAAATTTATTTGGAAGC+TGG - tig0027224:1722-1741 Msa1433030:CDS 30.0%
TATTTCACCATGCAATTATG+CGG + tig0027224:1776-1795 None:intergenic 30.0%
CATAATTGCATGGTGAAATA+GGG - tig0027224:1776-1795 Msa1433030:intron 30.0%
ATAATTGCATGGTGAAATAG+GGG - tig0027224:1777-1796 Msa1433030:intron 30.0%
TCTCAGTTACTCTATCATCA+TGG + tig0027224:713-732 None:intergenic 35.0%
AGTTGCATGTATGTATGACT+TGG + tig0027224:1020-1039 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG - tig0027224:1099-1118 Msa1433030:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG - tig0027224:1100-1119 Msa1433030:intron 35.0%
ATTGACACGGTAATATCACT+TGG + tig0027224:1129-1148 None:intergenic 35.0%
GATATTACCGTGTCAATTGA+TGG - tig0027224:1132-1151 Msa1433030:intron 35.0%
ATTGACACCATCAATTGACA+CGG + tig0027224:1142-1161 None:intergenic 35.0%
TGTCAATTTCCATCTTCACA+AGG - tig0027224:1155-1174 Msa1433030:intron 35.0%
ACAAAGTAGGGCACATTAAT+TGG + tig0027224:1331-1350 None:intergenic 35.0%
! GTTCACTTGATCTACTGTTT+TGG - tig0027224:1649-1668 Msa1433030:intron 35.0%
!!! CTACTGTTTTGGAAGTTATG+TGG - tig0027224:1660-1679 Msa1433030:intron 35.0%
ATTTCACCATGCAATTATGC+GGG + tig0027224:1775-1794 None:intergenic 35.0%
GCATAATTGCATGGTGAAAT+AGG - tig0027224:1775-1794 Msa1433030:intron 35.0%
! TTTCCTCTTGTTTCAAGATG+TGG - tig0027224:1917-1936 Msa1433030:intron 35.0%
TTTCCACATCTTGAAACAAG+AGG + tig0027224:1923-1942 None:intergenic 35.0%
GTTCTTCTCATAGGTATTCT+TGG + tig0027224:1969-1988 None:intergenic 35.0%
TCCTGAAACTTTCTTGATTG+TGG - tig0027224:2021-2040 Msa1433030:exon 35.0%
ATTGTGGCCAAATTCTGTTA+CGG - tig0027224:2037-2056 Msa1433030:five_prime_UTR 35.0%
TGCAGAATACCTTGAAATGA+CGG - tig0027224:2108-2127 Msa1433030:five_prime_UTR 35.0%
! CAACTGGAAAGATTGCATTT+TGG - tig0027224:2195-2214 Msa1433030:five_prime_UTR 35.0%
TCTCAGTTACTCTATCATCA+TGG + tig0027224:713-732 None:intergenic 35.0%
AGTTGCATGTATGTATGACT+TGG + tig0027224:1020-1039 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG - tig0027224:1099-1118 Msa1433030:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG - tig0027224:1100-1119 Msa1433030:intron 35.0%
ATTGACACGGTAATATCACT+TGG + tig0027224:1129-1148 None:intergenic 35.0%
GATATTACCGTGTCAATTGA+TGG - tig0027224:1132-1151 Msa1433030:intron 35.0%
ATTGACACCATCAATTGACA+CGG + tig0027224:1142-1161 None:intergenic 35.0%
TGTCAATTTCCATCTTCACA+AGG - tig0027224:1155-1174 Msa1433030:intron 35.0%
ACAAAGTAGGGCACATTAAT+TGG + tig0027224:1331-1350 None:intergenic 35.0%
! GTTCACTTGATCTACTGTTT+TGG - tig0027224:1649-1668 Msa1433030:intron 35.0%
!!! CTACTGTTTTGGAAGTTATG+TGG - tig0027224:1660-1679 Msa1433030:intron 35.0%
ATTTCACCATGCAATTATGC+GGG + tig0027224:1775-1794 None:intergenic 35.0%
GCATAATTGCATGGTGAAAT+AGG - tig0027224:1775-1794 Msa1433030:intron 35.0%
! TTTCCTCTTGTTTCAAGATG+TGG - tig0027224:1917-1936 Msa1433030:intron 35.0%
TTTCCACATCTTGAAACAAG+AGG + tig0027224:1923-1942 None:intergenic 35.0%
GTTCTTCTCATAGGTATTCT+TGG + tig0027224:1969-1988 None:intergenic 35.0%
TCCTGAAACTTTCTTGATTG+TGG - tig0027224:2021-2040 Msa1433030:exon 35.0%
ATTGTGGCCAAATTCTGTTA+CGG - tig0027224:2037-2056 Msa1433030:five_prime_UTR 35.0%
TGCAGAATACCTTGAAATGA+CGG - tig0027224:2108-2127 Msa1433030:five_prime_UTR 35.0%
! CAACTGGAAAGATTGCATTT+TGG - tig0027224:2195-2214 Msa1433030:five_prime_UTR 35.0%
CTTTCTGGGTTTCATCTTCA+AGG - tig0027224:866-885 Msa1433030:CDS 40.0%
TTCATCTTCAAGGTCATCAC+TGG - tig0027224:876-895 Msa1433030:CDS 40.0%
AAGTGCATACCTTGTGAAGA+TGG + tig0027224:1167-1186 None:intergenic 40.0%
GTAGGGCACATTAATTGGAT+TGG + tig0027224:1326-1345 None:intergenic 40.0%
! ACGTATGATTGGTTGTGAAG+GGG + tig0027224:1419-1438 None:intergenic 40.0%
! CACGTATGATTGGTTGTGAA+GGG + tig0027224:1420-1439 None:intergenic 40.0%
! ACACGTATGATTGGTTGTGA+AGG + tig0027224:1421-1440 None:intergenic 40.0%
TGAATTCCCGCATAATTGCA+TGG - tig0027224:1766-1785 Msa1433030:intron 40.0%
TCTTGGAATCTTCACAGGTA+CGG + tig0027224:1952-1971 None:intergenic 40.0%
GGTATTCTTGGAATCTTCAC+AGG + tig0027224:1957-1976 None:intergenic 40.0%
GCCACAATCAAGAAAGTTTC+AGG + tig0027224:2025-2044 None:intergenic 40.0%
TTGTGGCCAAATTCTGTTAC+GGG - tig0027224:2038-2057 Msa1433030:five_prime_UTR 40.0%
ACGAAACCCGTAACAGAATT+TGG + tig0027224:2047-2066 None:intergenic 40.0%
CGTATTCATCCGTCATTTCA+AGG + tig0027224:2120-2139 None:intergenic 40.0%
!! ATGACGGATGAATACGAAGA+AGG - tig0027224:2124-2143 Msa1433030:five_prime_UTR 40.0%
CTTTCTGGGTTTCATCTTCA+AGG - tig0027224:866-885 Msa1433030:CDS 40.0%
TTCATCTTCAAGGTCATCAC+TGG - tig0027224:876-895 Msa1433030:CDS 40.0%
AAGTGCATACCTTGTGAAGA+TGG + tig0027224:1167-1186 None:intergenic 40.0%
GTAGGGCACATTAATTGGAT+TGG + tig0027224:1326-1345 None:intergenic 40.0%
! ACGTATGATTGGTTGTGAAG+GGG + tig0027224:1419-1438 None:intergenic 40.0%
! CACGTATGATTGGTTGTGAA+GGG + tig0027224:1420-1439 None:intergenic 40.0%
! ACACGTATGATTGGTTGTGA+AGG + tig0027224:1421-1440 None:intergenic 40.0%
TGAATTCCCGCATAATTGCA+TGG - tig0027224:1766-1785 Msa1433030:intron 40.0%
TCTTGGAATCTTCACAGGTA+CGG + tig0027224:1952-1971 None:intergenic 40.0%
GGTATTCTTGGAATCTTCAC+AGG + tig0027224:1957-1976 None:intergenic 40.0%
GCCACAATCAAGAAAGTTTC+AGG + tig0027224:2025-2044 None:intergenic 40.0%
TTGTGGCCAAATTCTGTTAC+GGG - tig0027224:2038-2057 Msa1433030:five_prime_UTR 40.0%
ACGAAACCCGTAACAGAATT+TGG + tig0027224:2047-2066 None:intergenic 40.0%
CGTATTCATCCGTCATTTCA+AGG + tig0027224:2120-2139 None:intergenic 40.0%
!! ATGACGGATGAATACGAAGA+AGG - tig0027224:2124-2143 Msa1433030:five_prime_UTR 40.0%
!!! GTGGAGCCATTTTGAAGCAA+AGG + tig0027224:838-857 None:intergenic 45.0%
CTTGGAATCTTCACAGGTAC+GGG + tig0027224:1951-1970 None:intergenic 45.0%
CACTGTCAGGTTCTTCTCAT+AGG + tig0027224:1978-1997 None:intergenic 45.0%
CTATGAGAAGAACCTGACAG+TGG - tig0027224:1976-1995 Msa1433030:intron 45.0%
! TGGTGCTGGAAGAATTTCCT+GGG - tig0027224:1996-2015 Msa1433030:CDS 45.0%
!!! GTGGAGCCATTTTGAAGCAA+AGG + tig0027224:838-857 None:intergenic 45.0%
CTTGGAATCTTCACAGGTAC+GGG + tig0027224:1951-1970 None:intergenic 45.0%
CACTGTCAGGTTCTTCTCAT+AGG + tig0027224:1978-1997 None:intergenic 45.0%
CTATGAGAAGAACCTGACAG+TGG - tig0027224:1976-1995 Msa1433030:intron 45.0%
! TGGTGCTGGAAGAATTTCCT+GGG - tig0027224:1996-2015 Msa1433030:CDS 45.0%
!! AAAAAAAATACATAATAATT+TGG - tig0027224:682-701 Msa1433030:CDS 5.0%
!! AAAAAAAATACATAATAATT+TGG - tig0027224:682-701 Msa1433030:CDS 5.0%
TGCTTCAAAATGGCTCCACC+TGG - tig0027224:839-858 Msa1433030:CDS 50.0%
GCTTCAAAATGGCTCCACCT+GGG - tig0027224:840-859 Msa1433030:CDS 50.0%
TGAAACCCAGAAAGCTTCCC+AGG + tig0027224:860-879 None:intergenic 50.0%
ATTCTTCCAGCACCACTGTC+AGG + tig0027224:1991-2010 None:intergenic 50.0%
! GTGGTGCTGGAAGAATTTCC+TGG - tig0027224:1995-2014 Msa1433030:CDS 50.0%
! GGTGCTGGAAGAATTTCCTG+GGG - tig0027224:1997-2016 Msa1433030:CDS 50.0%
GTGCTGGAAGAATTTCCTGG+GGG - tig0027224:1998-2017 Msa1433030:CDS 50.0%
AAGAAAGTTTCAGGACCCCC+AGG + tig0027224:2016-2035 None:intergenic 50.0%
! CAAGGTATTCTGCAGCACAG+TGG + tig0027224:2102-2121 None:intergenic 50.0%
TGCTTCAAAATGGCTCCACC+TGG - tig0027224:839-858 Msa1433030:CDS 50.0%
GCTTCAAAATGGCTCCACCT+GGG - tig0027224:840-859 Msa1433030:CDS 50.0%
TGAAACCCAGAAAGCTTCCC+AGG + tig0027224:860-879 None:intergenic 50.0%
ATTCTTCCAGCACCACTGTC+AGG + tig0027224:1991-2010 None:intergenic 50.0%
! GTGGTGCTGGAAGAATTTCC+TGG - tig0027224:1995-2014 Msa1433030:CDS 50.0%
! GGTGCTGGAAGAATTTCCTG+GGG - tig0027224:1997-2016 Msa1433030:CDS 50.0%
GTGCTGGAAGAATTTCCTGG+GGG - tig0027224:1998-2017 Msa1433030:CDS 50.0%
AAGAAAGTTTCAGGACCCCC+AGG + tig0027224:2016-2035 None:intergenic 50.0%
! CAAGGTATTCTGCAGCACAG+TGG + tig0027224:2102-2121 None:intergenic 50.0%
CTCCACCTGGGAAGCTTTCT+GGG - tig0027224:852-871 Msa1433030:CDS 55.0%
AACCCAGAAAGCTTCCCAGG+TGG + tig0027224:857-876 None:intergenic 55.0%
GAAGAACCTGACAGTGGTGC+TGG - tig0027224:1982-2001 Msa1433030:intron 55.0%
CTCCACCTGGGAAGCTTTCT+GGG - tig0027224:852-871 Msa1433030:CDS 55.0%
AACCCAGAAAGCTTCCCAGG+TGG + tig0027224:857-876 None:intergenic 55.0%
GAAGAACCTGACAGTGGTGC+TGG - tig0027224:1982-2001 Msa1433030:intron 55.0%
GCTCCACCTGGGAAGCTTTC+TGG - tig0027224:851-870 Msa1433030:CDS 60.0%
GCTCCACCTGGGAAGCTTTC+TGG - tig0027224:851-870 Msa1433030:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0027224 gene 651 2231 651 ID=Msa1433030;Name=Msa1433030
tig0027224 mRNA 651 2231 651 ID=Msa1433030-mRNA-1;Parent=Msa1433030;Name=Msa1433030-mRNA-1;_AED=0.27;_eAED=0.27;_QI=197|1|0.66|1|1|1|3|0|144
tig0027224 exon 651 965 651 ID=Msa1433030-mRNA-1:exon:8470;Parent=Msa1433030-mRNA-1
tig0027224 exon 1706 1775 1706 ID=Msa1433030-mRNA-1:exon:8469;Parent=Msa1433030-mRNA-1
tig0027224 exon 1985 2231 1985 ID=Msa1433030-mRNA-1:exon:8468;Parent=Msa1433030-mRNA-1
tig0027224 five_prime_UTR 2035 2231 2035 ID=Msa1433030-mRNA-1:five_prime_utr;Parent=Msa1433030-mRNA-1
tig0027224 CDS 1985 2034 1985 ID=Msa1433030-mRNA-1:cds;Parent=Msa1433030-mRNA-1
tig0027224 CDS 1706 1775 1706 ID=Msa1433030-mRNA-1:cds;Parent=Msa1433030-mRNA-1
tig0027224 CDS 651 965 651 ID=Msa1433030-mRNA-1:cds;Parent=Msa1433030-mRNA-1
Gene Sequence

>Msa1433030

ATGGCTCCACCTGGGAAGCTTTCTGGGTTTCATCTTCAAGGTCATCACTGGTTTAGTGATTCTGGGTTGCCAAGTGATATTACCGTGTCAATTGATGGTGTCAATTTCCATCTTCACAAGTTTCCTCTTGTTTCAAGATGTGGAAAAATTGCCCGTACCTGTGAAGATTCCAAGAATACCTATGAGAAGAACCTGACAGTGGTGCTGGAAGAATTTCCTGGGGGTCCTGAAACTTTCTTGATTGTGGCCAAATTCTGTTACGGGTTTCGTGTAGAGCTGACAGCGAAAAATGTAGTATTAGTCCACTGTGCTGCAGAATACCTTGAAATGACGGATGAATACGAAGAAGGCAACTTGTTGACAAAGTCAGAGAGCTTTTTCCATAAAAATATATTGCGCAACTGGAAAGATTGCATTTTGGCTCCAAAGCTCTGA
Protein sequence

>Msa1433030

MAPPGKLSGFHLQGHHWFSDSGLPSDITVSIDGVNFHLHKFPLVSRCGKIARTCEDSKNTYEKNLTVVLEEFPGGPETFLIVAKFCYGFRVELTAKNVVLVHCAAEYLEMTDEYEEGNLLTKSESFFHKNILRNWKDCILAPKL*