AlfalfaGEDB | Msa1433200

M. sativa cultivar zhongmu-4 / Msa1433200

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433200	RHN80374.1	89.116	147	15	1	1	147	1	146	7.56e-90	268

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433200	A0A396JSX4	89.116	147	15	1	1	147	1	146	3.61e-90	268

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TFs/TRs:

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Gene ID	Type	Classification
Msa1433200	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433200	MtrunA17_Chr1g0187541	89.116	147	15	1	1	147	1	146	6.95e-94	268
Msa1433200	MtrunA17_Chr1g0187511	42.177	147	66	7	11	147	8	145	1.41e-23	89.7
Msa1433200	MtrunA17_Chr4g0031161	36.047	172	73	6	11	147	13	182	6.21e-23	89.0
Msa1433200	MtrunA17_Chr1g0187701	36.810	163	69	10	11	147	15	169	6.96e-19	78.2
Msa1433200	MtrunA17_Chr1g0166461	34.375	160	78	7	9	147	14	167	6.21e-13	62.8
Msa1433200	MtrunA17_Chr5g0423491	32.576	132	74	3	5	128	10	134	5.09e-11	57.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 31 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCCATATAATCTTCA+TGG	0.356028	tig0027239:-33620	None:intergenic
AAAGGTGGTGCTTCAATATG+AGG	0.426497	tig0027239:-33462	None:intergenic
AACTCATATGGAGTCTCATA+TGG	0.462152	tig0027239:+33398	Msa1433200:CDS
AACTTCCATATAATCTTCAT+GGG	0.524548	tig0027239:-33619	None:intergenic
AAGAAAATTTAGCCCTAAGC+AGG	0.461361	tig0027239:+33424	Msa1433200:CDS
AAGGTGGTGCTTCAATATGA+GGG	0.646397	tig0027239:-33461	None:intergenic
AATGTTGGTTAGCCTGCTTA+GGG	0.479077	tig0027239:-33436	None:intergenic
ACATGGTATGCCATCTGCTG+AGG	0.623247	tig0027239:+33557	Msa1433200:CDS
ACCAACCCATGAAGATTATA+TGG	0.323053	tig0027239:+33614	Msa1433200:CDS
ATACCATGTTGTAAAATATG+AGG	0.569652	tig0027239:-33543	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCGATTTCTCATAACTCATA+TGG	+	tig0027239:33386-33405	Msa1433200:CDS	30.0%
TTCAATATGAGGGTTAATGT+TGG	-	tig0027239:33454-33473	None:intergenic	30.0%
TGCATTTCACTAAACATTCT+TGG	-	tig0027239:33501-33520	None:intergenic	30.0%
AACTCATATGGAGTCTCATA+TGG	+	tig0027239:33398-33417	Msa1433200:CDS	35.0%
AAGAAAATTTAGCCCTAAGC+AGG	+	tig0027239:33424-33443	Msa1433200:CDS	35.0%
CCATGTTGTAAAATATGAGG+TGG	-	tig0027239:33543-33562	None:intergenic	35.0%
ACCAACCCATGAAGATTATA+TGG	+	tig0027239:33614-33633	Msa1433200:CDS	35.0%
TCCATATAATCTTCATGGGT+TGG	-	tig0027239:33618-33637	None:intergenic	35.0%
ATGGAAGTTTCTCACATTGA+TGG	+	tig0027239:33633-33652	Msa1433200:CDS	35.0%
AAGGTGGTGCTTCAATATGA+GGG	-	tig0027239:33464-33483	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0027239	gene	33318	33761	33318	ID=Msa1433200;Name=Msa1433200
tig0027239	mRNA	33318	33761	33318	ID=Msa1433200-mRNA-1;Parent=Msa1433200;Name=Msa1433200-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|147
tig0027239	exon	33318	33761	33318	ID=Msa1433200-mRNA-1:exon:8483;Parent=Msa1433200-mRNA-1
tig0027239	CDS	33318	33761	33318	ID=Msa1433200-mRNA-1:cds;Parent=Msa1433200-mRNA-1

Gene Sequence

>Msa1433200

ATGGCTAACAATAATGATAGCAATAATACTATTGCATGCAAATTGTGCTACAAAGTTTTCACAAACGTTCGATTTCTCATAACTCATATGGAGTCTCATATGGTTGAAGAAAATTTAGCCCTAAGCAGGCTAACCAACATTAACCCTCATATTGAAGCACCACCTTTGACAATACCTCGACCAAGAATGTTTAGTGAAATGCATGCTGCTGTTGTTTCACATCCACCTCATATTTTACAACATGGTATGCCATCTGCTGAGGAAAATGTTGCTGGAATGGCTTTGTCACCAACTCCACCAACCCATGAAGATTATATGGAAGTTTCTCACATTGATGGAACTAAGCCATATATCAATAAACTAGATAAACCCATCGATAAGATTATTGATAATCCAGCTATTGTGATCAATGATGGCACATTGAATCTAGAATTAAGGCTCTAA

Protein sequence

>Msa1433200

MANNNDSNNTIACKLCYKVFTNVRFLITHMESHMVEENLALSRLTNINPHIEAPPLTIPRPRMFSEMHAAVVSHPPHILQHGMPSAEENVAGMALSPTPPTHEDYMEVSHIDGTKPYINKLDKPIDKIIDNPAIVINDGTLNLELRL*