Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433200 | RHN80374.1 | 89.116 | 147 | 15 | 1 | 1 | 147 | 1 | 146 | 7.56e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433200 | A0A396JSX4 | 89.116 | 147 | 15 | 1 | 1 | 147 | 1 | 146 | 3.61e-90 | 268 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1433200 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433200 | MtrunA17_Chr1g0187541 | 89.116 | 147 | 15 | 1 | 1 | 147 | 1 | 146 | 6.95e-94 | 268 |
| Msa1433200 | MtrunA17_Chr1g0187511 | 42.177 | 147 | 66 | 7 | 11 | 147 | 8 | 145 | 1.41e-23 | 89.7 |
| Msa1433200 | MtrunA17_Chr4g0031161 | 36.047 | 172 | 73 | 6 | 11 | 147 | 13 | 182 | 6.21e-23 | 89.0 |
| Msa1433200 | MtrunA17_Chr1g0187701 | 36.810 | 163 | 69 | 10 | 11 | 147 | 15 | 169 | 6.96e-19 | 78.2 |
| Msa1433200 | MtrunA17_Chr1g0166461 | 34.375 | 160 | 78 | 7 | 9 | 147 | 14 | 167 | 6.21e-13 | 62.8 |
| Msa1433200 | MtrunA17_Chr5g0423491 | 32.576 | 132 | 74 | 3 | 5 | 128 | 10 | 134 | 5.09e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCTAGTTTATTGATATA+TGG | 0.244365 | tig0027239:-33663 | None:intergenic |
| TGCATTTCACTAAACATTCT+TGG | 0.273305 | tig0027239:-33498 | None:intergenic |
| ACCAACCCATGAAGATTATA+TGG | 0.323053 | tig0027239:+33614 | Msa1433200:CDS |
| GCACATTGAATCTAGAATTA+AGG | 0.324100 | tig0027239:+33733 | Msa1433200:CDS |
| TAATGTTGGTTAGCCTGCTT+AGG | 0.325076 | tig0027239:-33437 | None:intergenic |
| AAACTTCCATATAATCTTCA+TGG | 0.356028 | tig0027239:-33620 | None:intergenic |
| TCGATTTCTCATAACTCATA+TGG | 0.358118 | tig0027239:+33386 | Msa1433200:CDS |
| ATGGAAGTTTCTCACATTGA+TGG | 0.390703 | tig0027239:+33633 | Msa1433200:CDS |
| TCTGCTGAGGAAAATGTTGC+TGG | 0.421474 | tig0027239:+33570 | Msa1433200:CDS |
| AAAGGTGGTGCTTCAATATG+AGG | 0.426497 | tig0027239:-33462 | None:intergenic |
| AAGAAAATTTAGCCCTAAGC+AGG | 0.461361 | tig0027239:+33424 | Msa1433200:CDS |
| AACTCATATGGAGTCTCATA+TGG | 0.462152 | tig0027239:+33398 | Msa1433200:CDS |
| TCCATATAATCTTCATGGGT+TGG | 0.462544 | tig0027239:-33615 | None:intergenic |
| AATGTTGGTTAGCCTGCTTA+GGG | 0.479077 | tig0027239:-33436 | None:intergenic |
| CCATCATTGATCACAATAGC+TGG | 0.489184 | tig0027239:-33711 | None:intergenic |
| TCTTCATGGGTTGGTGGAGT+TGG | 0.495187 | tig0027239:-33606 | None:intergenic |
| CCAGCTATTGTGATCAATGA+TGG | 0.501173 | tig0027239:+33711 | Msa1433200:CDS |
| GCTATCATTATTGTTAGCCA+TGG | 0.507658 | tig0027239:-33316 | None:intergenic |
| ATTATCAATAATCTTATCGA+TGG | 0.515635 | tig0027239:-33688 | None:intergenic |
| TGAGGAAAATGTTGCTGGAA+TGG | 0.518703 | tig0027239:+33575 | Msa1433200:CDS |
| TCACTAAACATTCTTGGTCG+AGG | 0.522107 | tig0027239:-33492 | None:intergenic |
| AACTTCCATATAATCTTCAT+GGG | 0.524548 | tig0027239:-33619 | None:intergenic |
| TTCAATATGAGGGTTAATGT+TGG | 0.536185 | tig0027239:-33451 | None:intergenic |
| CTTGGTCGAGGTATTGTCAA+AGG | 0.560141 | tig0027239:-33480 | None:intergenic |
| TTATCAATAATCTTATCGAT+GGG | 0.560338 | tig0027239:-33687 | None:intergenic |
| ATACCATGTTGTAAAATATG+AGG | 0.569652 | tig0027239:-33543 | None:intergenic |
| ATATAATCTTCATGGGTTGG+TGG | 0.585977 | tig0027239:-33612 | None:intergenic |
| ACATGGTATGCCATCTGCTG+AGG | 0.623247 | tig0027239:+33557 | Msa1433200:CDS |
| GGTCGAGGTATTGTCAAAGG+TGG | 0.641328 | tig0027239:-33477 | None:intergenic |
| AAGGTGGTGCTTCAATATGA+GGG | 0.646397 | tig0027239:-33461 | None:intergenic |
| CCATGTTGTAAAATATGAGG+TGG | 0.673172 | tig0027239:-33540 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATCTAGTTTATTGATATA+TGG | - | tig0027239:33666-33685 | None:intergenic | 15.0% |
| !! | TTATCAATAATCTTATCGAT+GGG | - | tig0027239:33690-33709 | None:intergenic | 20.0% |
| !! | ATTATCAATAATCTTATCGA+TGG | - | tig0027239:33691-33710 | None:intergenic | 20.0% |
| ! | ATACCATGTTGTAAAATATG+AGG | - | tig0027239:33546-33565 | None:intergenic | 25.0% |
| ! | AACTTCCATATAATCTTCAT+GGG | - | tig0027239:33622-33641 | None:intergenic | 25.0% |
| ! | AAACTTCCATATAATCTTCA+TGG | - | tig0027239:33623-33642 | None:intergenic | 25.0% |
| TCGATTTCTCATAACTCATA+TGG | + | tig0027239:33386-33405 | Msa1433200:CDS | 30.0% | |
| TTCAATATGAGGGTTAATGT+TGG | - | tig0027239:33454-33473 | None:intergenic | 30.0% | |
| TGCATTTCACTAAACATTCT+TGG | - | tig0027239:33501-33520 | None:intergenic | 30.0% | |
| ! | GCACATTGAATCTAGAATTA+AGG | + | tig0027239:33733-33752 | Msa1433200:CDS | 30.0% |
| AACTCATATGGAGTCTCATA+TGG | + | tig0027239:33398-33417 | Msa1433200:CDS | 35.0% | |
| AAGAAAATTTAGCCCTAAGC+AGG | + | tig0027239:33424-33443 | Msa1433200:CDS | 35.0% | |
| CCATGTTGTAAAATATGAGG+TGG | - | tig0027239:33543-33562 | None:intergenic | 35.0% | |
| ! | CCACCTCATATTTTACAACA+TGG | + | tig0027239:33540-33559 | Msa1433200:CDS | 35.0% |
| !! | ATATAATCTTCATGGGTTGG+TGG | - | tig0027239:33615-33634 | None:intergenic | 35.0% |
| ACCAACCCATGAAGATTATA+TGG | + | tig0027239:33614-33633 | Msa1433200:CDS | 35.0% | |
| TCCATATAATCTTCATGGGT+TGG | - | tig0027239:33618-33637 | None:intergenic | 35.0% | |
| ATGGAAGTTTCTCACATTGA+TGG | + | tig0027239:33633-33652 | Msa1433200:CDS | 35.0% | |
| !! | AATGTTGGTTAGCCTGCTTA+GGG | - | tig0027239:33439-33458 | None:intergenic | 40.0% |
| !! | TAATGTTGGTTAGCCTGCTT+AGG | - | tig0027239:33440-33459 | None:intergenic | 40.0% |
| AAGGTGGTGCTTCAATATGA+GGG | - | tig0027239:33464-33483 | None:intergenic | 40.0% | |
| AAAGGTGGTGCTTCAATATG+AGG | - | tig0027239:33465-33484 | None:intergenic | 40.0% | |
| TCACTAAACATTCTTGGTCG+AGG | - | tig0027239:33495-33514 | None:intergenic | 40.0% | |
| TGAGGAAAATGTTGCTGGAA+TGG | + | tig0027239:33575-33594 | Msa1433200:CDS | 40.0% | |
| CCATCATTGATCACAATAGC+TGG | - | tig0027239:33714-33733 | None:intergenic | 40.0% | |
| CCAGCTATTGTGATCAATGA+TGG | + | tig0027239:33711-33730 | Msa1433200:CDS | 40.0% | |
| !! | CTTGGTCGAGGTATTGTCAA+AGG | - | tig0027239:33483-33502 | None:intergenic | 45.0% |
| ! | GCAACATTTTCCTCAGCAGA+TGG | - | tig0027239:33570-33589 | None:intergenic | 45.0% |
| TCTGCTGAGGAAAATGTTGC+TGG | + | tig0027239:33570-33589 | Msa1433200:CDS | 45.0% | |
| !! | GGTCGAGGTATTGTCAAAGG+TGG | - | tig0027239:33480-33499 | None:intergenic | 50.0% |
| ACATGGTATGCCATCTGCTG+AGG | + | tig0027239:33557-33576 | Msa1433200:CDS | 50.0% | |
| !! | TCTTCATGGGTTGGTGGAGT+TGG | - | tig0027239:33609-33628 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027239 | gene | 33318 | 33761 | 33318 | ID=Msa1433200;Name=Msa1433200 |
| tig0027239 | mRNA | 33318 | 33761 | 33318 | ID=Msa1433200-mRNA-1;Parent=Msa1433200;Name=Msa1433200-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|147 |
| tig0027239 | exon | 33318 | 33761 | 33318 | ID=Msa1433200-mRNA-1:exon:8483;Parent=Msa1433200-mRNA-1 |
| tig0027239 | CDS | 33318 | 33761 | 33318 | ID=Msa1433200-mRNA-1:cds;Parent=Msa1433200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
