Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435000 | KEH43825.1 | 68.293 | 82 | 24 | 1 | 249 | 328 | 4 | 85 | 1.22e-21 | 107 |
| Msa1435000 | KEH43825.1 | 87.395 | 238 | 22 | 4 | 410 | 640 | 4 | 240 | 1.09e-125 | 387 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435000 | sp|F4JIF5|TCX2_ARATH | 56.198 | 121 | 48 | 2 | 19 | 139 | 370 | 485 | 2.85e-33 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435000 | A0A072W0K6 | 68.293 | 82 | 24 | 1 | 249 | 328 | 4 | 85 | 5.82e-22 | 107 |
| Msa1435000 | A0A072W0K6 | 87.395 | 238 | 22 | 4 | 410 | 640 | 4 | 240 | 5.20e-126 | 387 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435000 | MtrunA17_Chr1g0205021 | 80.000 | 220 | 24 | 3 | 128 | 327 | 16 | 235 | 2.59e-109 | 334 |
| Msa1435000 | MtrunA17_Chr1g0205021 | 45.556 | 180 | 72 | 4 | 329 | 492 | 68 | 237 | 2.56e-32 | 126 |
| Msa1435000 | MtrunA17_Chr1g0205011 | 87.805 | 205 | 18 | 3 | 410 | 607 | 4 | 208 | 1.80e-107 | 328 |
| Msa1435000 | MtrunA17_Chr1g0205011 | 68.293 | 82 | 24 | 1 | 249 | 328 | 4 | 85 | 9.68e-26 | 106 |
| Msa1435000 | MtrunA17_Chr1g0205031 | 49.211 | 317 | 111 | 8 | 373 | 662 | 193 | 486 | 9.63e-74 | 251 |
| Msa1435000 | MtrunA17_Chr1g0205031 | 70.435 | 115 | 29 | 2 | 25 | 139 | 406 | 515 | 1.35e-43 | 167 |
| Msa1435000 | MtrunA17_Chr1g0205031 | 61.856 | 97 | 16 | 3 | 224 | 320 | 203 | 278 | 4.85e-23 | 104 |
| Msa1435000 | MtrunA17_Chr1g0205031 | 40.230 | 174 | 78 | 7 | 644 | 798 | 180 | 346 | 4.48e-18 | 89.0 |
| Msa1435000 | MtrunA17_Chr1g0205001 | 61.650 | 206 | 19 | 4 | 643 | 825 | 17 | 185 | 1.28e-67 | 223 |
| Msa1435000 | MtrunA17_Chr1g0205001 | 70.690 | 58 | 17 | 0 | 14 | 71 | 163 | 220 | 2.10e-19 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435000 | AT4G14770.1 | 56.198 | 121 | 48 | 2 | 19 | 139 | 370 | 485 | 2.90e-34 | 140 |
| Msa1435000 | AT3G22780.1 | 56.198 | 121 | 48 | 2 | 19 | 139 | 396 | 511 | 3.06e-34 | 140 |
| Msa1435000 | AT4G14770.2 | 56.198 | 121 | 48 | 2 | 19 | 139 | 350 | 465 | 3.33e-34 | 140 |
| Msa1435000 | AT4G14770.3 | 56.198 | 121 | 48 | 2 | 19 | 139 | 351 | 466 | 3.38e-34 | 140 |
| Msa1435000 | AT3G22760.2 | 53.390 | 118 | 50 | 2 | 22 | 139 | 203 | 315 | 3.89e-32 | 131 |
| Msa1435000 | AT3G22760.1 | 53.390 | 118 | 50 | 2 | 22 | 139 | 326 | 438 | 1.10e-31 | 132 |
| Msa1435000 | AT3G04850.1 | 48.413 | 126 | 60 | 2 | 21 | 146 | 450 | 570 | 2.70e-30 | 127 |
| Msa1435000 | AT5G25790.3 | 43.925 | 107 | 54 | 3 | 24 | 130 | 95 | 195 | 9.22e-17 | 84.3 |
| Msa1435000 | AT5G25790.1 | 41.121 | 107 | 61 | 2 | 24 | 130 | 95 | 199 | 7.73e-16 | 81.6 |
| Msa1435000 | AT5G25790.2 | 41.121 | 107 | 61 | 2 | 24 | 130 | 95 | 199 | 7.86e-16 | 81.6 |
Find 169 sgRNAs with CRISPR-Local
Find 1399 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATAAACCTGATACAGATC+TGG | 0.488763 | tig0027611:-11373 | Msa1435000:CDS |
| AAATGTCTTTCCATTGATAT+AGG | 0.344746 | tig0027611:+8439 | None:intergenic |
| AACAGACATTGTTCAAACAC+AGG | 0.615137 | tig0027611:-9326 | Msa1435000:CDS |
| AACTTCTTCTGGAAGGCAAC+CGG | 0.429598 | tig0027611:-8527 | Msa1435000:CDS |
| AAGCAGTCTTGACATGAGCA+AGG | 0.654291 | tig0027611:+30736 | None:intergenic |
| AAGGGATACCAATCAAAGTC+GGG | 0.476339 | tig0027611:-9281 | Msa1435000:CDS |
| AATAAAATATATTCACATGA+AGG | 0.545430 | tig0027611:-21817 | Msa1435000:CDS |
| AATCAAAGTCGGGGAGATGA+AGG | 0.477011 | tig0027611:-9271 | Msa1435000:CDS |
| AATGGTATTTACTTAAAACA+TGG | 0.595861 | tig0027611:-20897 | Msa1435000:CDS |
| AATTGTCTTTCCATTTGTGA+AGG | 0.532237 | tig0027611:+12144 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCAGCTTACTAAAATGA+AGG | + | tig0027611:8498-8517 | None:intergenic | 30.0% | |
| TTTATATCTAGGGGATGTAA+AGG | + | tig0027611:8563-8582 | None:intergenic | 30.0% | |
| TCACAGATAAAGCTACTAAT+TGG | - | tig0027611:9313-9332 | Msa1435000:CDS | 30.0% | |
| CCATTACCTTATTCATCAAT+CGG | - | tig0027611:9359-9378 | Msa1435000:CDS | 30.0% | |
| ATTTAAGCACGAGATCTTTA+CGG | + | tig0027611:9389-9408 | None:intergenic | 30.0% | |
| ATATCTTAAAATATGCCCCA+TGG | - | tig0027611:9807-9826 | Msa1435000:intron | 30.0% | |
| TATATCGATATAACACCATG+GGG | + | tig0027611:9825-9844 | None:intergenic | 30.0% | |
| GTTATATCGATATAACACCA+TGG | + | tig0027611:9827-9846 | None:intergenic | 30.0% | |
| ATTGCCATTCTTATTCATTG+CGG | + | tig0027611:10015-10034 | None:intergenic | 30.0% | |
| AATCAAAAGTCTCCAAAAAC+AGG | + | tig0027611:10305-10324 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027611 | gene | 8294 | 31261 | 8294 | ID=Msa1435000;Name=Msa1435000 |
| tig0027611 | mRNA | 8294 | 31261 | 8294 | ID=Msa1435000-mRNA-1;Parent=Msa1435000;Name=Msa1435000-mRNA-1;_AED=0.31;_eAED=0.32;_QI=0|0|0|1|0.5|0.46|15|0|825 |
| tig0027611 | exon | 31256 | 31261 | 31256 | ID=Msa1435000-mRNA-1:exon:9451;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 31134 | 31234 | 31134 | ID=Msa1435000-mRNA-1:exon:9450;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 30618 | 30798 | 30618 | ID=Msa1435000-mRNA-1:exon:9449;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 30435 | 30533 | 30435 | ID=Msa1435000-mRNA-1:exon:9448;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 21984 | 22090 | 21984 | ID=Msa1435000-mRNA-1:exon:9447;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 21728 | 21888 | 21728 | ID=Msa1435000-mRNA-1:exon:9446;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 21615 | 21703 | 21615 | ID=Msa1435000-mRNA-1:exon:9445;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 20881 | 21120 | 20881 | ID=Msa1435000-mRNA-1:exon:9444;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 12892 | 13134 | 12892 | ID=Msa1435000-mRNA-1:exon:9443;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 12249 | 12496 | 12249 | ID=Msa1435000-mRNA-1:exon:9442;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 12125 | 12236 | 12125 | ID=Msa1435000-mRNA-1:exon:9441;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 11909 | 12118 | 11909 | ID=Msa1435000-mRNA-1:exon:9440;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 11320 | 11448 | 11320 | ID=Msa1435000-mRNA-1:exon:9439;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 9271 | 9407 | 9271 | ID=Msa1435000-mRNA-1:exon:9438;Parent=Msa1435000-mRNA-1 |
| tig0027611 | exon | 8294 | 8708 | 8294 | ID=Msa1435000-mRNA-1:exon:9437;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 31256 | 31261 | 31256 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 31134 | 31234 | 31134 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 30618 | 30798 | 30618 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 30435 | 30533 | 30435 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 21984 | 22090 | 21984 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 21728 | 21888 | 21728 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 21615 | 21703 | 21615 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 20881 | 21120 | 20881 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 12892 | 13134 | 12892 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 12249 | 12496 | 12249 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 12125 | 12236 | 12125 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 11909 | 12118 | 11909 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 11320 | 11448 | 11320 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 9271 | 9407 | 9271 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| tig0027611 | CDS | 8294 | 8708 | 8294 | ID=Msa1435000-mRNA-1:cds;Parent=Msa1435000-mRNA-1 |
| Gene Sequence |
>Msa1435000 ATGTTACCTAACACCAAAGTTCGACTTTGCAGGAACACGTCAGATCTAAATAGGAAAGGAGGGGCATGCAAACACTGTAAATGCAAAAAATCAAAATGTTTGAAACTTAATTGTGGCTGCTTTGCTGCTGGAGTCTACTGCACAGGACCTTGCTCATGTCAAGACTGCTTGAATAAACATATTAATGAAGATACAGTTCTGCAAGCTCGCAGGATGATTGAGTATCATAATCCACCTGCATTTGTTCCTAAAGTTCGAAAGAATTCTGGTTCCTCACCTCAAATTGTGGATGACCCCAACAAAGCTCCAGCTTCAAAGCGACAACGCAGAATACGATGCAAGTGCAGGAAATCAAGCTGCATGAATAAACATTGTGAATGTTTTAAGAGCAAAAGGTATGATGGAGTTGGAGCTACTACCACGTTGAACTCGCTTGGCATGGATTATTCTCTTTTGAATTCACCTGACCCGATCGATTTAAAGGACTACACGAGGAATAACCTTTTGGACAAATCAATGCATCAAGTTTCATCTGGGAATGTAAATAAAATATATTCACATGAAGGAGCTCTTACAAATTCTTCTCATGCACGCCGTCATTCAAGTGAGCGACATCATCTTCTTGGATTACTTGGCAAGAAAGATTCAGTTGAGGGTCAAAAAAATACTAATAAAAGTCGGAACAATCTTGCAAATGTTGCTTTAATGGATAGGAATCCAATTGAGAGAAGGAGTGATGATGAGCTTGCACGTATTAGACGCTGTACACAAAGGAGCTGTCAAGGCTATGAGTCTGAGACGACCAGTTTGCAAAGGAACAACTCTGTTTATACTTCAAACATTAATTCCTGTTCAGCAAAATCTAATGAGAGGAATGCTAGTAATGAAAAGACATTACTTCCTAGAAAAAACACAAGTGATTCACAGATATGTGTTTTACAAGGAAATGGTATTTACTTAAAACATGGAAAGGAAGAGGAAAATGATATAAATAATATTAATTCACATGAAGAAGCTCCAACAGATTCAACTCATGCATGGATTGACACAAGTGAGCTACCTGATCCTCAAATCTCAATTGATGAAATTAACTATGTCCTTGGATTACTTGACGGCTCAGAACCAATTGAGGGTGCAACAAATATTCGGGAAGATAATCAAACACGGGACAATCTTGCCCATGTTGCTTTAATGGATAACAACCCATTTCAGAGAGTGGATGATGAGCTTGCACGTATTAGGCGCTGTGTAGAGAGGAGCTGTCAAGACTATGCGTCTGAGATGACCAGTTTGCAAAGGAACAACTATGCTTCTACTTTAAACATCAGTTCTTGTACAACAGAATCTGACAAGAGGAATGCTTTCAATGAAAAGCCCGTGCTTCCTAGAAAACACACTAGTGATCCACAAAGCTGTGTTTTGCAAGCAAATGGTTCTGGCTTATATCATTACAATGGCAAAGAAAACGAAAATATAAAAACTGAATCGCAAAGCTCCTCTTCTTTGCATCTCTCTGCAAGGCAAGGGCATTGCCTATCACTGGTCCCTTCACAAATGGAAAGACAATTGGGGCCATCAGAGAATGAAGCTACAAAAGATTTCGGTCGGAGTACCCCGAAAAGGACAATGAAAGTGGAGTTCAAGAACATAACGAATCAACAGTTAAATCAAAACATGAGAAGGACAATGATAGCGCAGGGGAAACGTCCGATTGATCCATCTTCTTCTGGTTCAAATCATGAAAAGGAAGAGGAAAATACAGATTTATGTCAGAGTATCCCCCAAAGGAAAAGGAAATGCTTTGCTGCTGGAGTCTACTGCAAAGGACCTTGCAGATGTAAAAACTGCGAAAATAAACCTGATACAGATCTGGAAGCTCGCACGGAGATTGAGTCTTGCAAACCACCTGCATTTGTTCCTAAAGCTGGTGTTGCTTCTGGTTCAACACATGGAATCGGAGAAAATGCTTCTGAACCAATGGCTGCAACAGACATTGTTCAAACACAGGGAAATCTTTCCAATGTTGCTTTAAGGGATACCAATCAAAGTCGGGGAGATGAAGGCAAAAAGTATGATGGAGTTGGAGCTACTATGACGTTGAACTCTCTTGGCATGGATTATCCTCTTGTTAATTCACCTCCAGCCTTGAATGCGCAGAGGGTTACTTTGCCAGGAAATGATTTTGACTTGAATGTACCTTATGATGGCATAGAAAATGAAATAACTTCTTCTGGAAGGCAACCGGAACTGCCGAACTACGCTGCTTCTTTGCATATTTATGAGAGCCAAGGGCATCAACTACCGCAGGCGCCTATATCAATGGAAAGACATTTCGGGCCATCTGAGATTGAAGTCAAGGCTACCACAGATTTCACTCAGTCATCAGTTCACATTTGCAGGCATGAGTCAGATCTAAGTGGGGAAGAAGGGACATGCAAAAACTGTCACTGTAAGAAATTGAAGTGTTTCAAACTGTAA |
| Protein sequence |
>Msa1435000 MLPNTKVRLCRNTSDLNRKGGACKHCKCKKSKCLKLNCGCFAAGVYCTGPCSCQDCLNKHINEDTVLQARRMIEYHNPPAFVPKVRKNSGSSPQIVDDPNKAPASKRQRRIRCKCRKSSCMNKHCECFKSKRYDGVGATTTLNSLGMDYSLLNSPDPIDLKDYTRNNLLDKSMHQVSSGNVNKIYSHEGALTNSSHARRHSSERHHLLGLLGKKDSVEGQKNTNKSRNNLANVALMDRNPIERRSDDELARIRRCTQRSCQGYESETTSLQRNNSVYTSNINSCSAKSNERNASNEKTLLPRKNTSDSQICVLQGNGIYLKHGKEEENDINNINSHEEAPTDSTHAWIDTSELPDPQISIDEINYVLGLLDGSEPIEGATNIREDNQTRDNLAHVALMDNNPFQRVDDELARIRRCVERSCQDYASEMTSLQRNNYASTLNISSCTTESDKRNAFNEKPVLPRKHTSDPQSCVLQANGSGLYHYNGKENENIKTESQSSSSLHLSARQGHCLSLVPSQMERQLGPSENEATKDFGRSTPKRTMKVEFKNITNQQLNQNMRRTMIAQGKRPIDPSSSGSNHEKEEENTDLCQSIPQRKRKCFAAGVYCKGPCRCKNCENKPDTDLEARTEIESCKPPAFVPKAGVASGSTHGIGENASEPMAATDIVQTQGNLSNVALRDTNQSRGDEGKKYDGVGATMTLNSLGMDYPLVNSPPALNAQRVTLPGNDFDLNVPYDGIENEITSSGRQPELPNYAASLHIYESQGHQLPQAPISMERHFGPSEIEVKATTDFTQSSVHICRHESDLSGEEGTCKNCHCKKLKCFKL* |
