AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1436060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1436060 MtrunA17_Chr8g0337271 82.653 196 34 0 1 196 1 196 1.20e-120 339
Msa1436060 MtrunA17_Chr8g0358731 34.328 201 95 6 1 199 1 166 4.03e-26 99.0
Msa1436060 MtrunA17_Chr6g0452691 27.211 147 100 2 10 149 9 155 3.67e-12 62.4
Msa1436060 MtrunA17_Chr5g0439111 27.660 141 96 4 10 148 9 145 1.17e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1436060 AT3G50510.1 28.696 115 81 1 1 114 3 117 3.14e-14 68.6
Msa1436060 AT3G50510.2 28.696 115 81 1 1 114 3 117 3.14e-14 68.6
Msa1436060 AT2G19820.1 31.183 93 63 1 5 96 7 99 7.57e-13 63.2

Find 51 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCAAGGATGTGCTTATT+TGG 0.063042 tig0027849:+15329 None:intergenic
TCAGTGTGTTATTCAAATTT+TGG 0.145999 tig0027849:-15494 Msa1436060:CDS
CTGACTTAAACTTGAGTATA+TGG 0.197296 tig0027849:-15213 Msa1436060:CDS
CATGACACTGATTGTGAATT+TGG 0.306396 tig0027849:-15550 Msa1436060:CDS
ATCCAAGGATGTGCTTATTT+GGG 0.326625 tig0027849:+15330 None:intergenic
TCTTGGACACCAATTGGAGC+TGG 0.391570 tig0027849:-15356 Msa1436060:CDS
AAAGTCTTCATCCTCATTAC+TGG 0.397078 tig0027849:+14750 None:intergenic
TTCATCAGCCACCATTCCCT+TGG 0.409411 tig0027849:+14807 None:intergenic
TGTCCAAGAAAACCCCGATC+TGG 0.424943 tig0027849:+15370 None:intergenic
TTTGGGCACCCAGCTCCAAT+TGG 0.428496 tig0027849:+15347 None:intergenic
TTAATTGCATAATTCTCATC+AGG 0.430232 tig0027849:+15461 None:intergenic
GGGAATGGTGGCTGATGAAA+AGG 0.430935 tig0027849:-14803 Msa1436060:CDS
TGACTTAAACTTGAGTATAT+GGG 0.443514 tig0027849:-15212 Msa1436060:intron
CTTGGACACCAATTGGAGCT+GGG 0.456606 tig0027849:-15355 Msa1436060:CDS
TTGGCGAAGTAACCCAGATC+GGG 0.470404 tig0027849:-15383 Msa1436060:CDS
TGCCCAAATAAGCACATCCT+TGG 0.475102 tig0027849:-15332 Msa1436060:CDS
AGCAATTTCAATTTCATCCA+AGG 0.478006 tig0027849:+15315 None:intergenic
CATATACTCAAGTTTAAGTC+AGG 0.479889 tig0027849:+15214 None:intergenic
TCTCCTCTTTCACAAGTACA+TGG 0.497829 tig0027849:+14963 None:intergenic
TCCTTCTGTGAGAAGCGAAT+CGG 0.511515 tig0027849:+15411 None:intergenic
TACTGAACACATGGGTGACA+TGG 0.514848 tig0027849:-15015 Msa1436060:CDS
GCCGATTCGCTTCTCACAGA+AGG 0.517177 tig0027849:-15412 Msa1436060:CDS
ATTCCATGTACTTGTGAAAG+AGG 0.523001 tig0027849:-14966 Msa1436060:CDS
TGTACACAGTAATGCATCCA+AGG 0.526625 tig0027849:-14824 Msa1436060:intron
GTCCAAGAAAACCCCGATCT+GGG 0.526848 tig0027849:+15371 None:intergenic
CTGAAAGTCAGCATGCCTAC+TGG 0.528158 tig0027849:+15513 None:intergenic
TGTGCCGACATATGAGAAGA+AGG 0.531308 tig0027849:-15573 Msa1436060:CDS
GTGGCTGATGAAAAGGGAAA+AGG 0.537842 tig0027849:-14796 Msa1436060:CDS
CTTGGCGAAGTAACCCAGAT+CGG 0.540526 tig0027849:-15384 Msa1436060:CDS
CATGCCTTCTTCTCATATGT+CGG 0.541385 tig0027849:+15569 None:intergenic
GTCTTCATCCTCATTACTGG+TGG 0.547436 tig0027849:+14753 None:intergenic
GCAACAATTTGTCTTTGATG+TGG 0.547669 tig0027849:+15433 None:intergenic
TTCTCACAGAAGGAGAAGCT+TGG 0.549947 tig0027849:-15402 Msa1436060:CDS
GTGCAAGAAGGCATGGAAGA+AGG 0.556829 tig0027849:+15244 None:intergenic
CAGTAATGCATCCAAGGGAA+TGG 0.575632 tig0027849:-14818 Msa1436060:CDS
AATTTGAGTGCAAGAAGGCA+TGG 0.581783 tig0027849:+15237 None:intergenic
AAATGCATCAGAGAAATACA+AGG 0.593981 tig0027849:-15612 Msa1436060:CDS
TAATGCATCCAAGGGAATGG+TGG 0.611462 tig0027849:-14815 Msa1436060:CDS
ACAAGGACCCACCAGTAATG+AGG 0.613595 tig0027849:-14761 Msa1436060:CDS
CTTGTGAAAGAGGAGAATCA+AGG 0.617523 tig0027849:-14956 Msa1436060:intron
GGAATGGTGGCTGATGAAAA+GGG 0.618900 tig0027849:-14802 Msa1436060:CDS
CATGGGTGACATGGACATCA+CGG 0.621521 tig0027849:-15006 Msa1436060:CDS
TCAGGAATTTGAGTGCAAGA+AGG 0.621914 tig0027849:+15232 None:intergenic
TTAATAGAGTACTGAACACA+TGG 0.626411 tig0027849:-15024 Msa1436060:intron
GTACACAGTAATGCATCCAA+GGG 0.631300 tig0027849:-14823 Msa1436060:intron
TCTTCATCCTCATTACTGGT+GGG 0.637097 tig0027849:+14754 None:intergenic
AAAGGAATTCTTAATGAACA+AGG 0.657272 tig0027849:-14778 Msa1436060:CDS
TGGCGAAGTAACCCAGATCG+GGG 0.667644 tig0027849:-15382 Msa1436060:CDS
ATCACGGAACAAGACATGCA+AGG 0.684891 tig0027849:-14990 Msa1436060:CDS
AATGCATCAGAGAAATACAA+GGG 0.687321 tig0027849:-15611 Msa1436060:CDS
TAATAGAGTACTGAACACAT+GGG 0.740993 tig0027849:-15023 Msa1436060:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAAAAGAATGATAGATTT+TGG + tig0027849:15286-15305 None:intergenic 20.0%
!!! TTGAAAAGAATGATAGATTT+TGG + tig0027849:15286-15305 None:intergenic 20.0%
!!! TCAGTGTGTTATTCAAATTT+TGG - tig0027849:14860-14879 Msa1436060:intron 25.0%
!!! CAGTGTGTTATTCAAATTTT+GGG - tig0027849:14861-14880 Msa1436060:intron 25.0%
!!! AGTGTGTTATTCAAATTTTG+GGG - tig0027849:14862-14881 Msa1436060:intron 25.0%
! TTAATTGCATAATTCTCATC+AGG + tig0027849:14896-14915 None:intergenic 25.0%
! TGACTTAAACTTGAGTATAT+GGG - tig0027849:15142-15161 Msa1436060:intron 25.0%
! AAAGGAATTCTTAATGAACA+AGG - tig0027849:15576-15595 Msa1436060:CDS 25.0%
!!! TCAGTGTGTTATTCAAATTT+TGG - tig0027849:14860-14879 Msa1436060:intron 25.0%
!!! CAGTGTGTTATTCAAATTTT+GGG - tig0027849:14861-14880 Msa1436060:intron 25.0%
!!! AGTGTGTTATTCAAATTTTG+GGG - tig0027849:14862-14881 Msa1436060:intron 25.0%
! TTAATTGCATAATTCTCATC+AGG + tig0027849:14896-14915 None:intergenic 25.0%
! TGACTTAAACTTGAGTATAT+GGG - tig0027849:15142-15161 Msa1436060:intron 25.0%
! AAAGGAATTCTTAATGAACA+AGG - tig0027849:15576-15595 Msa1436060:CDS 25.0%
AGCAATTTCAATTTCATCCA+AGG + tig0027849:15042-15061 None:intergenic 30.0%
CATATACTCAAGTTTAAGTC+AGG + tig0027849:15143-15162 None:intergenic 30.0%
CTGACTTAAACTTGAGTATA+TGG - tig0027849:15141-15160 Msa1436060:intron 30.0%
ATGTGTTCAGTACTCTATTA+AGG + tig0027849:15331-15350 None:intergenic 30.0%
!! TTAATAGAGTACTGAACACA+TGG - tig0027849:15330-15349 Msa1436060:CDS 30.0%
!! TAATAGAGTACTGAACACAT+GGG - tig0027849:15331-15350 Msa1436060:CDS 30.0%
AGCAATTTCAATTTCATCCA+AGG + tig0027849:15042-15061 None:intergenic 30.0%
CATATACTCAAGTTTAAGTC+AGG + tig0027849:15143-15162 None:intergenic 30.0%
CTGACTTAAACTTGAGTATA+TGG - tig0027849:15141-15160 Msa1436060:intron 30.0%
ATGTGTTCAGTACTCTATTA+AGG + tig0027849:15331-15350 None:intergenic 30.0%
!! TTAATAGAGTACTGAACACA+TGG - tig0027849:15330-15349 Msa1436060:CDS 30.0%
!! TAATAGAGTACTGAACACAT+GGG - tig0027849:15331-15350 Msa1436060:CDS 30.0%
! CATGACACTGATTGTGAATT+TGG - tig0027849:14804-14823 Msa1436060:CDS 35.0%
GCAACAATTTGTCTTTGATG+TGG + tig0027849:14924-14943 None:intergenic 35.0%
ATCCAAGGATGTGCTTATTT+GGG + tig0027849:15027-15046 None:intergenic 35.0%
ATTCCATGTACTTGTGAAAG+AGG - tig0027849:15388-15407 Msa1436060:CDS 35.0%
AAAGTCTTCATCCTCATTAC+TGG + tig0027849:15607-15626 None:intergenic 35.0%
CTTGTATTTCTCTGATGCAT+TGG + tig0027849:14744-14763 None:intergenic 35.0%
! CATGACACTGATTGTGAATT+TGG - tig0027849:14804-14823 Msa1436060:CDS 35.0%
GCAACAATTTGTCTTTGATG+TGG + tig0027849:14924-14943 None:intergenic 35.0%
ATCCAAGGATGTGCTTATTT+GGG + tig0027849:15027-15046 None:intergenic 35.0%
ATTCCATGTACTTGTGAAAG+AGG - tig0027849:15388-15407 Msa1436060:CDS 35.0%
AAAGTCTTCATCCTCATTAC+TGG + tig0027849:15607-15626 None:intergenic 35.0%
CATGCCTTCTTCTCATATGT+CGG + tig0027849:14788-14807 None:intergenic 40.0%
! GTCAATACTTTTCGACCAGT+AGG - tig0027849:14826-14845 Msa1436060:intron 40.0%
CATCCAAGGATGTGCTTATT+TGG + tig0027849:15028-15047 None:intergenic 40.0%
AATTTGAGTGCAAGAAGGCA+TGG + tig0027849:15120-15139 None:intergenic 40.0%
TCAGGAATTTGAGTGCAAGA+AGG + tig0027849:15125-15144 None:intergenic 40.0%
TCTCCTCTTTCACAAGTACA+TGG + tig0027849:15394-15413 None:intergenic 40.0%
CTTGTGAAAGAGGAGAATCA+AGG - tig0027849:15398-15417 Msa1436060:CDS 40.0%
TGTACACAGTAATGCATCCA+AGG - tig0027849:15530-15549 Msa1436060:CDS 40.0%
GTACACAGTAATGCATCCAA+GGG - tig0027849:15531-15550 Msa1436060:CDS 40.0%
TCTTCATCCTCATTACTGGT+GGG + tig0027849:15603-15622 None:intergenic 40.0%
CATGCCTTCTTCTCATATGT+CGG + tig0027849:14788-14807 None:intergenic 40.0%
! GTCAATACTTTTCGACCAGT+AGG - tig0027849:14826-14845 Msa1436060:intron 40.0%
CATCCAAGGATGTGCTTATT+TGG + tig0027849:15028-15047 None:intergenic 40.0%
AATTTGAGTGCAAGAAGGCA+TGG + tig0027849:15120-15139 None:intergenic 40.0%
TCAGGAATTTGAGTGCAAGA+AGG + tig0027849:15125-15144 None:intergenic 40.0%
TCTCCTCTTTCACAAGTACA+TGG + tig0027849:15394-15413 None:intergenic 40.0%
CTTGTGAAAGAGGAGAATCA+AGG - tig0027849:15398-15417 Msa1436060:CDS 40.0%
TGTACACAGTAATGCATCCA+AGG - tig0027849:15530-15549 Msa1436060:CDS 40.0%
GTACACAGTAATGCATCCAA+GGG - tig0027849:15531-15550 Msa1436060:CDS 40.0%
TCTTCATCCTCATTACTGGT+GGG + tig0027849:15603-15622 None:intergenic 40.0%
! TGTGCCGACATATGAGAAGA+AGG - tig0027849:14781-14800 Msa1436060:CDS 45.0%
TCCTTCTGTGAGAAGCGAAT+CGG + tig0027849:14946-14965 None:intergenic 45.0%
TTCTCACAGAAGGAGAAGCT+TGG - tig0027849:14952-14971 Msa1436060:intron 45.0%
! GGGTTTTCTTGGACACCAAT+TGG - tig0027849:14992-15011 Msa1436060:CDS 45.0%
TGCCCAAATAAGCACATCCT+TGG - tig0027849:15022-15041 Msa1436060:intron 45.0%
TACTGAACACATGGGTGACA+TGG - tig0027849:15339-15358 Msa1436060:CDS 45.0%
ATCACGGAACAAGACATGCA+AGG - tig0027849:15364-15383 Msa1436060:CDS 45.0%
CAGTAATGCATCCAAGGGAA+TGG - tig0027849:15536-15555 Msa1436060:CDS 45.0%
TAATGCATCCAAGGGAATGG+TGG - tig0027849:15539-15558 Msa1436060:CDS 45.0%
GGAATGGTGGCTGATGAAAA+GGG - tig0027849:15552-15571 Msa1436060:CDS 45.0%
GTGGCTGATGAAAAGGGAAA+AGG - tig0027849:15558-15577 Msa1436060:CDS 45.0%
GTCTTCATCCTCATTACTGG+TGG + tig0027849:15604-15623 None:intergenic 45.0%
! TGTGCCGACATATGAGAAGA+AGG - tig0027849:14781-14800 Msa1436060:CDS 45.0%
TCCTTCTGTGAGAAGCGAAT+CGG + tig0027849:14946-14965 None:intergenic 45.0%
TTCTCACAGAAGGAGAAGCT+TGG - tig0027849:14952-14971 Msa1436060:intron 45.0%
! GGGTTTTCTTGGACACCAAT+TGG - tig0027849:14992-15011 Msa1436060:CDS 45.0%
TGCCCAAATAAGCACATCCT+TGG - tig0027849:15022-15041 Msa1436060:intron 45.0%
TACTGAACACATGGGTGACA+TGG - tig0027849:15339-15358 Msa1436060:CDS 45.0%
ATCACGGAACAAGACATGCA+AGG - tig0027849:15364-15383 Msa1436060:CDS 45.0%
CAGTAATGCATCCAAGGGAA+TGG - tig0027849:15536-15555 Msa1436060:CDS 45.0%
TAATGCATCCAAGGGAATGG+TGG - tig0027849:15539-15558 Msa1436060:CDS 45.0%
GGAATGGTGGCTGATGAAAA+GGG - tig0027849:15552-15571 Msa1436060:CDS 45.0%
GTGGCTGATGAAAAGGGAAA+AGG - tig0027849:15558-15577 Msa1436060:CDS 45.0%
GTCTTCATCCTCATTACTGG+TGG + tig0027849:15604-15623 None:intergenic 45.0%
CTGAAAGTCAGCATGCCTAC+TGG + tig0027849:14844-14863 None:intergenic 50.0%
CTTGGCGAAGTAACCCAGAT+CGG - tig0027849:14970-14989 Msa1436060:CDS 50.0%
TTGGCGAAGTAACCCAGATC+GGG - tig0027849:14971-14990 Msa1436060:CDS 50.0%
! AACCCAGATCGGGGTTTTCT+TGG - tig0027849:14981-15000 Msa1436060:CDS 50.0%
GTCCAAGAAAACCCCGATCT+GGG + tig0027849:14986-15005 None:intergenic 50.0%
TGTCCAAGAAAACCCCGATC+TGG + tig0027849:14987-15006 None:intergenic 50.0%
TCTTGGACACCAATTGGAGC+TGG - tig0027849:14998-15017 Msa1436060:CDS 50.0%
CTTGGACACCAATTGGAGCT+GGG - tig0027849:14999-15018 Msa1436060:CDS 50.0%
! GTGCAAGAAGGCATGGAAGA+AGG + tig0027849:15113-15132 None:intergenic 50.0%
CATGGGTGACATGGACATCA+CGG - tig0027849:15348-15367 Msa1436060:CDS 50.0%
TTCATCAGCCACCATTCCCT+TGG + tig0027849:15550-15569 None:intergenic 50.0%
GGGAATGGTGGCTGATGAAA+AGG - tig0027849:15551-15570 Msa1436060:CDS 50.0%
ACAAGGACCCACCAGTAATG+AGG - tig0027849:15593-15612 Msa1436060:CDS 50.0%
CTGAAAGTCAGCATGCCTAC+TGG + tig0027849:14844-14863 None:intergenic 50.0%
CTTGGCGAAGTAACCCAGAT+CGG - tig0027849:14970-14989 Msa1436060:CDS 50.0%
TTGGCGAAGTAACCCAGATC+GGG - tig0027849:14971-14990 Msa1436060:CDS 50.0%
! AACCCAGATCGGGGTTTTCT+TGG - tig0027849:14981-15000 Msa1436060:CDS 50.0%
GTCCAAGAAAACCCCGATCT+GGG + tig0027849:14986-15005 None:intergenic 50.0%
TGTCCAAGAAAACCCCGATC+TGG + tig0027849:14987-15006 None:intergenic 50.0%
TCTTGGACACCAATTGGAGC+TGG - tig0027849:14998-15017 Msa1436060:CDS 50.0%
CTTGGACACCAATTGGAGCT+GGG - tig0027849:14999-15018 Msa1436060:CDS 50.0%
! GTGCAAGAAGGCATGGAAGA+AGG + tig0027849:15113-15132 None:intergenic 50.0%
CATGGGTGACATGGACATCA+CGG - tig0027849:15348-15367 Msa1436060:CDS 50.0%
TTCATCAGCCACCATTCCCT+TGG + tig0027849:15550-15569 None:intergenic 50.0%
GGGAATGGTGGCTGATGAAA+AGG - tig0027849:15551-15570 Msa1436060:CDS 50.0%
ACAAGGACCCACCAGTAATG+AGG - tig0027849:15593-15612 Msa1436060:CDS 50.0%
!! GCCGATTCGCTTCTCACAGA+AGG - tig0027849:14942-14961 Msa1436060:intron 55.0%
TGGCGAAGTAACCCAGATCG+GGG - tig0027849:14972-14991 Msa1436060:CDS 55.0%
TTTGGGCACCCAGCTCCAAT+TGG + tig0027849:15010-15029 None:intergenic 55.0%
!! GCCGATTCGCTTCTCACAGA+AGG - tig0027849:14942-14961 Msa1436060:intron 55.0%
TGGCGAAGTAACCCAGATCG+GGG - tig0027849:14972-14991 Msa1436060:CDS 55.0%
TTTGGGCACCCAGCTCCAAT+TGG + tig0027849:15010-15029 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0027849 gene 14744 15632 14744 ID=Msa1436060;Name=Msa1436060
tig0027849 mRNA 14744 15632 14744 ID=Msa1436060-mRNA-1;Parent=Msa1436060;Name=Msa1436060-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|0.66|3|0|199
tig0027849 exon 15213 15632 15213 ID=Msa1436060-mRNA-1:exon:10060;Parent=Msa1436060-mRNA-1
tig0027849 exon 14957 15039 14957 ID=Msa1436060-mRNA-1:exon:10059;Parent=Msa1436060-mRNA-1
tig0027849 exon 14744 14837 14744 ID=Msa1436060-mRNA-1:exon:10058;Parent=Msa1436060-mRNA-1
tig0027849 CDS 15213 15632 15213 ID=Msa1436060-mRNA-1:cds;Parent=Msa1436060-mRNA-1
tig0027849 CDS 14957 15039 14957 ID=Msa1436060-mRNA-1:cds;Parent=Msa1436060-mRNA-1
tig0027849 CDS 14744 14837 14744 ID=Msa1436060-mRNA-1:cds;Parent=Msa1436060-mRNA-1
Gene Sequence

>Msa1436060

ATGCATCAGAGAAATACAAGGGCTATTACATGTCTTTTGTGCCGACATATGAGAAGAAGGCATGACACTGATTGTGAATTTGGTCAATACTTTTCGACCAGTAGGCATGCTGACTTTCAGTGTGTTATTCAAATTTTGGGGTTAAGTCGCCTGATGAGAATTATGCAATTAATAGAACCACATCAAAGACAAATTGTTGCCGATTCGCTTCTCACAGAAGGAGAAGCTTGGCGAAGTAACCCAGATCGGGGTTTTCTTGGACACCAATTGGAGCTGGGTGCCCAAATAAGCACATCCTTGGATGAAATTGAAATTGCTAGAAGACTTTTAGCTTTTTGCATTGATAATGCAAATTCAAACAATGCACCTTCTTCCATGCCTTCTTGCACTCAAATTCCTGACTTAAACTTGAGTATATGGAGTACTGAACACATGGGTGACATGGACATCACGGAACAAGACATGCAAGGTATTCCATGTACTTGTGAAAGAGGAGAATCAAGTAATGCATCCAAGGGAATGGTGGCTGATGAAAAGGGAAAAGGAATTCTTAATGAACAAGGACCCACCAGTAATGAGGATGAAGACTTTTCCCCA

Protein sequence

>Msa1436060

MHQRNTRAITCLLCRHMRRRHDTDCEFGQYFSTSRHADFQCVIQILGLSRLMRIMQLIEPHQRQIVADSLLTEGEAWRSNPDRGFLGHQLELGAQISTSLDEIEIARRLLAFCIDNANSNNAPSSMPSCTQIPDLNLSIWSTEHMGDMDITEQDMQGIPCTCERGESSNASKGMVADEKGKGILNEQGPTSNEDEDFSP