AlfalfaGEDB | Msa1437290

M. sativa cultivar zhongmu-4 / Msa1437290

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0098542
PFAM: PF12819.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437290	RHN76308.1	77.841	176	26	2	4	166	28	203	1.20e-88	271

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437290	sp\|O65924\|Y2921_ARATH	43.353	173	83	3	5	162	264	436	9.02e-35	135
Msa1437290	sp\|O65924\|Y2921_ARATH	50.000	100	49	1	150	249	549	647	4.12e-22	98.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437290	A0A396JJ12	77.841	176	26	2	4	166	28	203	5.73e-89	271

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437290	MtrunA17_Chr2g0330621	77.841	176	26	2	4	166	28	203	1.10e-92	271
Msa1437290	MtrunA17_Chr2g0330591	73.034	178	34	3	4	168	261	437	4.68e-80	257
Msa1437290	MtrunA17_Chr2g0330591	71.963	107	29	1	143	249	541	646	3.22e-43	155
Msa1437290	MtrunA17_Chr2g0330631	89.655	87	9	0	163	249	73	159	4.79e-50	164
Msa1437290	MtrunA17_Chr8g0340871	41.436	181	89	5	4	168	265	444	7.23e-31	120
Msa1437290	MtrunA17_Chr8g0340871	48.810	84	43	0	166	249	565	648	3.33e-20	89.4
Msa1437290	MtrunA17_Chr8g0341151	40.217	184	87	5	4	168	255	434	8.26e-30	117
Msa1437290	MtrunA17_Chr8g0341151	51.724	87	42	0	163	249	546	632	1.41e-22	96.3
Msa1437290	MtrunA17_Chr8g0340931	40.659	182	90	5	4	168	259	439	1.23e-28	114
Msa1437290	MtrunA17_Chr8g0340931	52.874	87	41	0	163	249	553	639	2.52e-22	95.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1437290	AT2G19210.1	43.353	173	83	3	5	162	264	436	9.17e-36	135
Msa1437290	AT2G19210.1	50.000	100	49	1	150	249	549	647	4.19e-23	98.2
Msa1437290	AT1G05700.1	41.358	162	78	5	21	166	276	436	8.31e-29	114
Msa1437290	AT1G05700.1	45.783	83	44	1	167	249	560	641	1.18e-16	79.3
Msa1437290	AT1G05700.2	41.358	162	78	5	21	166	276	436	9.68e-29	114
Msa1437290	AT1G05700.2	45.783	83	44	1	167	249	591	672	1.17e-16	79.7
Msa1437290	AT1G51890.5	60.920	87	33	1	163	249	284	369	1.01e-28	114
Msa1437290	AT1G51890.5	38.217	157	78	4	25	162	1	157	3.69e-20	89.7
Msa1437290	AT1G51890.4	60.920	87	33	1	163	249	492	577	1.27e-28	114
Msa1437290	AT1G51890.4	38.650	163	81	4	19	162	203	365	3.83e-22	95.5

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Find 54 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGCAGCTATGTTGGTA+CGG	0.585202	tig0028256:-40854	Msa1437290:CDS
AAATACTTGACAGGTCTTAT+AGG	0.242464	tig0028256:-38058	Msa1437290:CDS
AAGTAAGACACTGGAGTACA+AGG	0.521279	tig0028256:+40889	None:intergenic
AATACTTGACAGGTCTTATA+GGG	0.376188	tig0028256:-38057	Msa1437290:CDS
ACTCTAGATTAGAGCCATGT+TGG	0.527280	tig0028256:+38018	None:intergenic
ACTTACAAGTATATGATCCT+TGG	0.514030	tig0028256:+40826	None:intergenic
AGCATTAACCATGACATGAG+GGG	0.634567	tig0028256:-42641	None:intergenic
AGTTATTAGTGAAACGAAGT+TGG	0.493413	tig0028256:-42305	Msa1437290:CDS
ATATCTGCAATTTCAAGCAG+AGG	0.641697	tig0028256:-38222	Msa1437290:intron
ATATGTGGAGGCATTATACT+TGG	0.487647	tig0028256:+42465	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGATACAAAGGAATGAAT+AGG	+	tig0028256:38303-38322	None:intergenic	30.0%
AGTTATTAGTGAAACGAAGT+TGG	-	tig0028256:38342-38361	Msa1437290:CDS	30.0%
CATGTAAATAATGAAGTGCA+TGG	+	tig0028256:38541-38560	None:intergenic	30.0%
CATGCACTTCATTATTTACA+TGG	-	tig0028256:38539-38558	Msa1437290:intron	30.0%
ACCAAACCGAAAATTTCTTA+GGG	+	tig0028256:38634-38653	None:intergenic	30.0%
AACCAAACCGAAAATTTCTT+AGG	+	tig0028256:39577-39596	None:intergenic	30.0%
TGGAAAATTAATTTCACACG+TGG	+	tig0028256:38765-38784	None:intergenic	30.0%
AAACCAAACCGAAAATTTCT+TGG	-	tig0028256:39576-39595	Msa1437290:intron	30.0%
AACCAAACCGAAAATTTCTT+GGG	-	tig0028256:39577-39596	Msa1437290:intron	30.0%
GGAAATAACCTATATTACAG+AGG	-	tig0028256:39609-39628	Msa1437290:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0028256	gene	38021	42648	38021	ID=Msa1437290;Name=Msa1437290
tig0028256	mRNA	38021	42648	38021	ID=Msa1437290-mRNA-1;Parent=Msa1437290;Name=Msa1437290-mRNA-1;_AED=0.24;_eAED=0.25;_QI=0\|0\|0\|0.83\|0.8\|0.66\|6\|0\|249
tig0028256	exon	42642	42648	42642	ID=Msa1437290-mRNA-1:exon:10605;Parent=Msa1437290-mRNA-1
tig0028256	exon	42197	42520	42197	ID=Msa1437290-mRNA-1:exon:10604;Parent=Msa1437290-mRNA-1
tig0028256	exon	40832	40946	40832	ID=Msa1437290-mRNA-1:exon:10603;Parent=Msa1437290-mRNA-1
tig0028256	exon	40694	40750	40694	ID=Msa1437290-mRNA-1:exon:10602;Parent=Msa1437290-mRNA-1
tig0028256	exon	38223	38382	38223	ID=Msa1437290-mRNA-1:exon:10601;Parent=Msa1437290-mRNA-1
tig0028256	exon	38021	38107	38021	ID=Msa1437290-mRNA-1:exon:10600;Parent=Msa1437290-mRNA-1
tig0028256	CDS	42642	42648	42642	ID=Msa1437290-mRNA-1:cds;Parent=Msa1437290-mRNA-1
tig0028256	CDS	42197	42520	42197	ID=Msa1437290-mRNA-1:cds;Parent=Msa1437290-mRNA-1
tig0028256	CDS	40832	40946	40832	ID=Msa1437290-mRNA-1:cds;Parent=Msa1437290-mRNA-1
tig0028256	CDS	40694	40750	40694	ID=Msa1437290-mRNA-1:cds;Parent=Msa1437290-mRNA-1
tig0028256	CDS	38223	38382	38223	ID=Msa1437290-mRNA-1:cds;Parent=Msa1437290-mRNA-1
tig0028256	CDS	38021	38107	38021	ID=Msa1437290-mRNA-1:cds;Parent=Msa1437290-mRNA-1

Gene Sequence

>Msa1437290

ATGAGGGCTGCCAGTGACAACTTGGAATTTTCCTTCCTTCCCAAGTATAATGCCTCCACATATTATGTGTACATGCACTTTGCTGAAATCCAGAAGCTCGATGCAAATCAAATTAGAGAGTTCAACATTTTTGTAAATGGGCAGCTTATAAATAGTGACCTATTCATTCCTTTGTATCTTCAGAACCTGTATTATATATCAGTTATTAGTGAAACGAAGTTGGAACTTTGGTTTAATAAGACTAGTAGATCAACTCTTCCACCACTATTCAATGTCGTCGAGACATATATGGCAAAGGACTTCTTACAATCAGAAACATATCAAACCGATGTCAACTTATGGGATCAAGAGAAACTGGCAGGAGATCCTTGTACTCCAGTGTCTTACTTGTGGAATGGCCTAAACTGCAGCTATGTTGGTACGGATTCGCCAAGGATCATATACTTCGGTTTAATTGGAACTATAGCTTCTGGGATATCCAATCTGAAGTCAATAGAATATTTCTATGAAGAAGTGTTAAACATTACCAGAAACTTTGAAAAGGTTGTGGAGAAAGGAGCATCTGGAACAGTCTACCATGGCTGGATTGATCATGATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCAACTCAAGGATATCTGCAATTTCAAGCAGAGGCCAAACTTTTTGCTGTAGTTCATCATAAATACTTGACAGGTCTTATAGGGTATTGTGATGATGGCACCAACATGGCTCTAATCTAG

Protein sequence

>Msa1437290

MRAASDNLEFSFLPKYNASTYYVYMHFAEIQKLDANQIREFNIFVNGQLINSDLFIPLYLQNLYYISVISETKLELWFNKTSRSTLPPLFNVVETYMAKDFLQSETYQTDVNLWDQEKLAGDPCTPVSYLWNGLNCSYVGTDSPRIIYFGLIGTIASGISNLKSIEYFYEEVLNITRNFEKVVEKGASGTVYHGWIDHDTEVAVKMLSSSSTQGYLQFQAEAKLFAVVHHKYLTGLIGYCDDGTNMALI*