AlfalfaGEDB | Msa1438260

M. sativa cultivar zhongmu-4 / Msa1438260

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF19160.3

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1438260	XP_003629966.1	94.560	625	34	0	26	650	1	625	0.0	1238

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1438260	sp\|O04086\|Y1105_ARATH	58.306	614	222	11	40	645	16	603	0.0	692

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1438260	A0A396GNV4	64.842	603	204	5	50	648	693	1291	0.0	805
Msa1438260	A0A396GNV4	94.560	625	34	0	26	650	1	625	0.0	1238

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1438260	MtrunA17_Chr8g0380681	94.560	625	34	0	26	650	1	625	0.0	1238
Msa1438260	MtrunA17_Chr8g0380681	64.842	603	204	5	50	648	693	1291	0.0	805
Msa1438260	MtrunA17_Chr2g0277731	63.548	620	209	7	33	646	12	620	0.0	792
Msa1438260	MtrunA17_Chr1g0159611	55.319	376	148	8	267	642	178	533	9.90e-135	406
Msa1438260	MtrunA17_Chr1g0159611	41.916	167	76	4	6	171	33	179	2.33e-31	129
Msa1438260	MtrunA17_Chr7g0259211	37.396	599	326	20	47	635	15	574	3.48e-105	333
Msa1438260	MtrunA17_Chr1g0196391	37.050	556	323	13	87	637	54	587	2.62e-103	328
Msa1438260	MtrunA17_Chr5g0413171	39.572	374	194	8	281	645	42	392	6.62e-73	241
Msa1438260	MtrunA17_Chr2g0315061	40.694	317	172	5	323	638	25	326	2.36e-72	238
Msa1438260	MtrunA17_Chr7g0236531	39.514	329	182	5	323	650	27	339	3.09e-72	237

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1438260	AT1G11050.1	58.306	614	222	11	40	645	16	603	0.0	692
Msa1438260	AT2G48010.1	35.889	613	342	16	31	637	7	574	2.10e-109	343
Msa1438260	AT5G45780.2	37.438	406	221	8	225	626	158	534	5.86e-73	246
Msa1438260	AT5G45780.1	37.438	406	221	8	225	626	201	577	1.57e-72	246
Msa1438260	AT3G09010.1	39.514	329	173	7	299	621	8	316	2.61e-69	231
Msa1438260	AT1G01540.2	42.763	304	157	5	322	624	141	428	3.07e-69	233
Msa1438260	AT4G01330.2	42.006	319	168	5	322	639	149	451	4.62e-69	233
Msa1438260	AT1G53430.2	38.965	367	201	7	268	633	568	912	4.77e-69	243
Msa1438260	AT1G53430.1	38.965	367	201	7	268	633	609	953	4.95e-69	243
Msa1438260	AT4G01330.3	42.763	304	157	5	322	624	149	436	7.60e-69	231

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Find 132 sgRNAs with CRISPR-Local

Find 342 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGAAGTGAGCTTAGTT+TGG	0.314069	tig0028533:+7014	None:intergenic
AACACATAATTCATGTCAAC+TGG	0.407774	tig0028533:+7226	None:intergenic
AACACCTAATTTCTTGATCA+CGG	0.508543	tig0028533:-5736	Msa1438260:CDS
AACTCCAACAATATAAATGA+TGG	0.510321	tig0028533:+6733	None:intergenic
AACTGGACATGAAGAATTGT+TGG	0.521928	tig0028533:+7243	None:intergenic
AACTGTTGCTGTTAAAAGCA+TGG	0.551392	tig0028533:-6321	Msa1438260:CDS
AAGAGCGATGTCTATAGCTT+TGG	0.485884	tig0028533:-5816	Msa1438260:CDS
AAGCATCCAAAGCTTCTGCT+AGG	0.565857	tig0028533:+5682	None:intergenic
AAGGGTTGCAGATTTCGGGC+TGG	0.558348	tig0028533:-5940	Msa1438260:CDS
AAGGTCATTGTTAAGTGAAA+GGG	0.495644	tig0028533:+6997	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATGAAATCTTAGCTATTTG+TGG	+	tig0028533:5506-5525	None:intergenic	30.0%
TTCATCAAGTGTCATACATA+TGG	-	tig0028533:5520-5539	Msa1438260:CDS	30.0%
GATCACTAAAACCACAAAAA+TGG	+	tig0028533:5549-5568	None:intergenic	30.0%
AACACATAATTCATGTCAAC+TGG	+	tig0028533:5635-5654	None:intergenic	30.0%
ATTATGTGTTAAGAGTTCCT+TGG	-	tig0028533:5645-5664	Msa1438260:CDS	30.0%
AAGGTCATTGTTAAGTGAAA+GGG	+	tig0028533:5864-5883	None:intergenic	30.0%
GTATTCAATCTAAGCAAGAT+TGG	-	tig0028533:5939-5958	Msa1438260:CDS	30.0%
GAGGATAAAGAGAATAAGTA+CGG	-	tig0028533:6330-6349	Msa1438260:CDS	30.0%
TTCTCAGTCCAAAACTATAT+TGG	-	tig0028533:6466-6485	Msa1438260:CDS	30.0%
TATACTGAAGTGGAGATTAT+TGG	-	tig0028533:6592-6611	Msa1438260:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0028533	gene	5464	7416	5464	ID=Msa1438260;Name=Msa1438260
tig0028533	mRNA	5464	7416	5464	ID=Msa1438260-mRNA-1;Parent=Msa1438260;Name=Msa1438260-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|650
tig0028533	exon	5464	7416	5464	ID=Msa1438260-mRNA-1:exon:11149;Parent=Msa1438260-mRNA-1
tig0028533	CDS	5464	7416	5464	ID=Msa1438260-mRNA-1:cds;Parent=Msa1438260-mRNA-1

Gene Sequence

>Msa1438260

ATGCATAAATACCCCCAAAAAACACAAAACCTTAAGACACCACAAATAGCTAAGATTTCATCAAGTGTCATACATATGGAGTCCATTTTTGTGGTTTTAGTGATCTTTGTTTTGTCTACAAATTCAATCTCAGCAGCTCCCTCTCCCTCTGCCAACAATTCTTCATGTCCAGTTGACATGAATTATGTGTTAAGAGTTCCTTGGAACACCTCAACATGCCTTAACTTCCAACCCTTGCAATCCCAAAACAAAGCACAAACAGCTCTATGTTGTCAAACTTTGTTGTCCCTTTTTGGGATAGCACTAGCAAAAAATCTCAAGAAGAACTCTCTTTTCCAACTCCCAAATCTTCCTACCTCTACCTCATGCCTTCAAGACTTCCAAACTAAGCTCACTTCCCTTTCACTTAACAATGACCTTGTGTCTTCATGCTTTGATCCACTTCAATTTGTTATCACTCCCAACATATGTGCTCGTATTCAATCTAAGCAAGATTGGATCAGCAGGGTTGGTCCTGCACCATTGTTCAACACTTCTTGTAAACCAGACCTTAGTGTTTCTACCAATTGTGATGCATGTGTTAACCAGGGACTAAAGGTTCAAGAGACGCTGAATCGAATTGATGGTAATAGTTCGCATTCCAAAGATTGCTTTTACTTCACCATCATTTATATTGTTGGAGTTGTGAATGAGTTTGGCCCTGAAAGCAACGGTGCTATGTCATGCATTCTTGAATTGCCTCTTAACTCTCAAGTGGGTTCCAGAAAAAAGAGCCATCATGCTCTTGTTTTTGGGTTAATTGGAGCTTCAGTCGGATTCATCGTTATCATGTTCTCTTTGTTTGCCTACTATTTTTGGCACACTAGGAGGATAAAGAGAATAAGTACGGAAAATTTTCTAGACCTTGCCGATCCAAGTGAACAAAGGTCCGCTCCAAGATTGAGACCAAAAACAGGATCAATCTGGTTCAAATTTGATGACCTTGTAAAGGCGACTAACAATTTCTCAGTCCAAAACTATATTGGTAGAGGTGGATTTGGGATAGTTTACAAGGGCATTCTACCTGATGGCACAACTGTTGCTGTTAAAAGCATGGAAGAATCTGATTTTCAAGGGGATGATGAGTTCTATACTGAAGTGGAGATTATTGGCAACTTGAAGCACCGCAGTCTAGTGCCACTAAGAGGGTGTTGTGTGGTTGATGAGGATCATAATCAAGAGCACAAAAATAGGTATCTTGTTTATGATTATATGCCAAATGGTAACCTTGAAGATCATCTCTTTCCATCCTCGGACAACAAAAACGAAAAGAAATTATTGACTTGGCCTCAAAGAAAAAACATAATCTTGGATGTGGCACGTGGGTTGGTTTATTTGCACTTTGGAGTTAAACCTGCTATTTATCACAGAGATATCAAAGCCACCAATATACTACTTGATGCAGATATGAGAGCAAGGGTTGCAGATTTCGGGCTGGCGAAAGAAAGCAGTGAAAGTAGGTCTCAACTAAATACAAGAGTAGCTGGAACTTATGGATATCTTGCTCCAGAATATGCACTCTATGGTCAGCTAACCGGGAAGAGCGATGTCTATAGCTTTGGTGTGGTTGTTTTGGAGATTATGTGTGGAAGAAAAGCTCTCGAGTCGTCTTCATCAGGAACACCTAATTTCTTGATCACGGATTGGGTTTGGTCGTTAATGAAATCAGGAAACCTAGCAGAAGCTTTGGATGCTTCTATCTTGGTGGATGATGGAAAATCCAATAGTAGAAATATAATGCAGAGGTTTTTGCTGGTTGGAATTCTGTCTTCTCATGTAATAGCTGATTCAAGGCCAACAATTTTGGATGCTTTGAAGATGTTAGAAGGAGATATTGAAGTTCCGCCAATTCCTGATAGGCCGATGACTCTTGAGCACCACATGTTTACTAATGATGATTCTGCAAAACAGTGA

Protein sequence

>Msa1438260

MHKYPQKTQNLKTPQIAKISSSVIHMESIFVVLVIFVLSTNSISAAPSPSANNSSCPVDMNYVLRVPWNTSTCLNFQPLQSQNKAQTALCCQTLLSLFGIALAKNLKKNSLFQLPNLPTSTSCLQDFQTKLTSLSLNNDLVSSCFDPLQFVITPNICARIQSKQDWISRVGPAPLFNTSCKPDLSVSTNCDACVNQGLKVQETLNRIDGNSSHSKDCFYFTIIYIVGVVNEFGPESNGAMSCILELPLNSQVGSRKKSHHALVFGLIGASVGFIVIMFSLFAYYFWHTRRIKRISTENFLDLADPSEQRSAPRLRPKTGSIWFKFDDLVKATNNFSVQNYIGRGGFGIVYKGILPDGTTVAVKSMEESDFQGDDEFYTEVEIIGNLKHRSLVPLRGCCVVDEDHNQEHKNRYLVYDYMPNGNLEDHLFPSSDNKNEKKLLTWPQRKNIILDVARGLVYLHFGVKPAIYHRDIKATNILLDADMRARVADFGLAKESSESRSQLNTRVAGTYGYLAPEYALYGQLTGKSDVYSFGVVVLEIMCGRKALESSSSGTPNFLITDWVWSLMKSGNLAEALDASILVDDGKSNSRNIMQRFLLVGILSSHVIADSRPTILDALKMLEGDIEVPPIPDRPMTLEHHMFTNDDSAKQ*