Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440450 | sp|Q9SY66|FRS11_ARATH | 34.356 | 163 | 86 | 5 | 45 | 192 | 34 | 190 | 4.16e-14 | 73.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440450 | A0A2Z6PEJ1 | 66.667 | 204 | 66 | 2 | 2 | 204 | 24 | 226 | 2.87e-95 | 291 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1440450 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440450 | MtrunA17_Chr1g0203051 | 35.938 | 192 | 110 | 1 | 25 | 203 | 38 | 229 | 3.85e-39 | 141 |
| Msa1440450 | MtrunA17_Chr1g0171741 | 36.782 | 174 | 108 | 2 | 4 | 176 | 83 | 255 | 6.20e-35 | 124 |
| Msa1440450 | MtrunA17_Chr8g0389811 | 37.736 | 159 | 98 | 1 | 6 | 164 | 38 | 195 | 1.40e-33 | 119 |
| Msa1440450 | MtrunA17_Chr2g0287651 | 36.250 | 160 | 102 | 0 | 44 | 203 | 66 | 225 | 1.30e-27 | 109 |
| Msa1440450 | MtrunA17_Chr2g0331371 | 37.342 | 158 | 92 | 1 | 8 | 158 | 3 | 160 | 2.98e-27 | 102 |
| Msa1440450 | MtrunA17_Chr6g0473421 | 36.641 | 131 | 76 | 1 | 8 | 131 | 3 | 133 | 1.45e-22 | 89.4 |
| Msa1440450 | MtrunA17_Chr1g0172931 | 36.893 | 103 | 65 | 0 | 33 | 135 | 56 | 158 | 1.94e-18 | 78.6 |
| Msa1440450 | MtrunA17_Chr1g0198761 | 33.858 | 127 | 72 | 5 | 59 | 175 | 53 | 177 | 1.74e-13 | 68.6 |
| Msa1440450 | MtrunA17_Chr2g0276841 | 34.014 | 147 | 82 | 4 | 59 | 196 | 76 | 216 | 2.05e-13 | 68.2 |
| Msa1440450 | MtrunA17_Chr5g0448761 | 31.818 | 154 | 103 | 2 | 51 | 202 | 58 | 211 | 3.34e-13 | 67.8 |
| Msa1440450 | MtrunA17_Chr2g0294841 | 30.968 | 155 | 89 | 5 | 59 | 201 | 51 | 199 | 2.08e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1440450 | AT1G10240.2 | 34.356 | 163 | 86 | 5 | 45 | 192 | 34 | 190 | 4.23e-15 | 73.6 |
| Msa1440450 | AT1G10240.1 | 34.356 | 163 | 86 | 5 | 45 | 192 | 34 | 190 | 4.23e-15 | 73.6 |
| Msa1440450 | AT5G28530.4 | 31.707 | 123 | 81 | 2 | 59 | 179 | 61 | 182 | 5.88e-14 | 70.1 |
| Msa1440450 | AT5G28530.5 | 31.707 | 123 | 81 | 2 | 59 | 179 | 61 | 182 | 6.28e-14 | 70.1 |
| Msa1440450 | AT5G28530.3 | 31.707 | 123 | 81 | 2 | 59 | 179 | 61 | 182 | 6.69e-14 | 70.1 |
| Msa1440450 | AT5G28530.2 | 31.707 | 123 | 81 | 2 | 59 | 179 | 61 | 182 | 6.69e-14 | 70.1 |
| Msa1440450 | AT5G28530.1 | 31.707 | 123 | 81 | 2 | 59 | 179 | 61 | 182 | 7.02e-14 | 70.1 |
| Msa1440450 | AT4G38180.1 | 30.769 | 156 | 105 | 3 | 49 | 202 | 69 | 223 | 1.10e-11 | 63.5 |
| Msa1440450 | AT3G59470.3 | 32.168 | 143 | 85 | 7 | 21 | 159 | 39 | 173 | 4.90e-11 | 60.8 |
| Msa1440450 | AT3G59470.1 | 32.168 | 143 | 85 | 7 | 21 | 159 | 39 | 173 | 4.90e-11 | 60.8 |
| Msa1440450 | AT3G59470.2 | 32.168 | 143 | 85 | 7 | 21 | 159 | 51 | 185 | 5.15e-11 | 60.8 |
Find 49 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAGGCAATCTATGTTTAT+TGG | 0.295916 | tig0029087:+24049 | None:intergenic |
| TACCAGTTCTACTACTGGTT+TGG | 0.296108 | tig0029087:-23947 | Msa1440450:CDS |
| ATATCAATCTTCAAAATATC+AGG | 0.302921 | tig0029087:+24028 | None:intergenic |
| GCATAGAAAATTGTCAAAAT+TGG | 0.303743 | tig0029087:-23642 | Msa1440450:CDS |
| GAATGAGTTGGACGAAGAAT+TGG | 0.337521 | tig0029087:-24122 | Msa1440450:CDS |
| GGTGCAATGGCAAATGCTTC+TGG | 0.358193 | tig0029087:-23563 | Msa1440450:CDS |
| ATTGGTTCCAAATCACCTTC+GGG | 0.363893 | tig0029087:+24067 | None:intergenic |
| TATTGGTTCCAAATCACCTT+CGG | 0.372238 | tig0029087:+24066 | None:intergenic |
| GAAGGTTTAAGCCATGGAAT+TGG | 0.373619 | tig0029087:-24100 | Msa1440450:CDS |
| ATTGCACCGTACATCTGATA+TGG | 0.395766 | tig0029087:+23578 | None:intergenic |
| ACCAGTTCTACTACTGGTTT+GGG | 0.402961 | tig0029087:-23946 | Msa1440450:CDS |
| AATTGGATGTACAGGATATT+CGG | 0.403668 | tig0029087:-23625 | Msa1440450:CDS |
| TACTGGTTTGGGAGAAGAAA+AGG | 0.407808 | tig0029087:-23935 | Msa1440450:CDS |
| ATTGTCAAAATTGGATGTAC+AGG | 0.424754 | tig0029087:-23633 | Msa1440450:CDS |
| TTCATAAGAGTCTGATGATA+AGG | 0.425264 | tig0029087:-23904 | Msa1440450:CDS |
| GTGAACACACATAGGTTTGT+TGG | 0.440149 | tig0029087:+23861 | None:intergenic |
| TCCCAAACCAGTAGTAGAAC+TGG | 0.465930 | tig0029087:+23945 | None:intergenic |
| GGGAGTTGTTTCCAATTCCA+TGG | 0.469694 | tig0029087:+24089 | None:intergenic |
| CTGCCTACCAGTTCTACTAC+TGG | 0.479288 | tig0029087:-23952 | Msa1440450:CDS |
| GTGGATTAACACCCGAGAAA+AGG | 0.491667 | tig0029087:-23817 | Msa1440450:CDS |
| ACCTATGTGTGTTCACGTGC+AGG | 0.495433 | tig0029087:-23854 | Msa1440450:CDS |
| GAAGTTGAGAGGTACAGCTT+TGG | 0.520939 | tig0029087:-23986 | Msa1440450:CDS |
| AACTGTGACATACCATCGCC+TGG | 0.526121 | tig0029087:+23724 | None:intergenic |
| GAATTGGAAGGTTTAAGCCA+TGG | 0.558266 | tig0029087:-24106 | Msa1440450:CDS |
| CACAACCATGTGCTGTTGGA+TGG | 0.560550 | tig0029087:-23689 | Msa1440450:CDS |
| TCTGATGATAAGGAATCGTA+CGG | 0.560905 | tig0029087:-23894 | Msa1440450:CDS |
| ACTCATTCTCTCCCGCATCA+TGG | 0.574153 | tig0029087:+24137 | None:intergenic |
| ATATCAGATGTACGGTGCAA+TGG | 0.583654 | tig0029087:-23576 | Msa1440450:CDS |
| TATCAATCTTCAAAATATCA+GGG | 0.588637 | tig0029087:+24029 | None:intergenic |
| GTACCTCTCAACTTCTTCGA+AGG | 0.600209 | tig0029087:+23994 | None:intergenic |
| ATAAGACCATATCAGATGTA+CGG | 0.603816 | tig0029087:-23584 | Msa1440450:CDS |
| TGATGCGGGAGAGAATGAGT+TGG | 0.604824 | tig0029087:-24134 | Msa1440450:CDS |
| TGGTCACAACCATGTGCTGT+TGG | 0.609196 | tig0029087:-23693 | Msa1440450:CDS |
| CTGATGATAAGGAATCGTAC+GGG | 0.620525 | tig0029087:-23893 | Msa1440450:CDS |
| TCATTCCATCCAACAGCACA+TGG | 0.625026 | tig0029087:+23684 | None:intergenic |
| TTGGTTCCAAATCACCTTCG+GGG | 0.629437 | tig0029087:+24068 | None:intergenic |
| TTTCCTTCGAAGAAGTTGAG+AGG | 0.631586 | tig0029087:-23997 | Msa1440450:CDS |
| TCCTGCACGTGAACACACAT+AGG | 0.636134 | tig0029087:+23853 | None:intergenic |
| TGGTTCCAAATCACCTTCGG+GGG | 0.641189 | tig0029087:+24069 | None:intergenic |
| TCGACATTCACACCAGGCGA+TGG | 0.642528 | tig0029087:-23736 | Msa1440450:CDS |
| GAGTTGGACGAAGAATTGGA+AGG | 0.645890 | tig0029087:-24118 | Msa1440450:CDS |
| ATTCGGAGTAATAACAACGT+TGG | 0.670677 | tig0029087:-23608 | Msa1440450:CDS |
| GCAGGATTTAGAGAAGATCG+TGG | 0.673352 | tig0029087:-23836 | Msa1440450:CDS |
| TACATGTCGACATTCACACC+AGG | 0.674089 | tig0029087:-23742 | Msa1440450:CDS |
| GCAATGGCAAATGCTTCTGG+TGG | 0.675264 | tig0029087:-23560 | Msa1440450:CDS |
| AAACCAGTAGTAGAACTGGT+AGG | 0.688245 | tig0029087:+23949 | None:intergenic |
| CTGTTGGATGGAATGATGTG+CGG | 0.697681 | tig0029087:-23677 | Msa1440450:CDS |
| GCTAGAAATGAAACAAGATG+CGG | 0.713432 | tig0029087:-23785 | Msa1440450:CDS |
| CAATCTTCAAAATATCAGGG+AGG | 0.740535 | tig0029087:+24032 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCAATCTTCAAAATATCA+GGG | + | tig0029087:23677-23696 | None:intergenic | 20.0% |
| !! | ATATCAATCTTCAAAATATC+AGG | + | tig0029087:23678-23697 | None:intergenic | 20.0% |
| !! | TATGTCACAGTTTTTGAATT+TGG | - | tig0029087:23990-24009 | Msa1440450:CDS | 25.0% |
| ! | GCATAGAAAATTGTCAAAAT+TGG | - | tig0029087:24061-24080 | Msa1440450:CDS | 25.0% |
| TTCATAAGAGTCTGATGATA+AGG | - | tig0029087:23799-23818 | Msa1440450:CDS | 30.0% | |
| ATTGTCAAAATTGGATGTAC+AGG | - | tig0029087:24070-24089 | Msa1440450:CDS | 30.0% | |
| AATTGGATGTACAGGATATT+CGG | - | tig0029087:24078-24097 | Msa1440450:CDS | 30.0% | |
| ATAAGACCATATCAGATGTA+CGG | - | tig0029087:24119-24138 | Msa1440450:CDS | 30.0% | |
| !! | TATTGGTTCCAAATCACCTT+CGG | + | tig0029087:23640-23659 | None:intergenic | 35.0% |
| CAATCTTCAAAATATCAGGG+AGG | + | tig0029087:23674-23693 | None:intergenic | 35.0% | |
| TCTGATGATAAGGAATCGTA+CGG | - | tig0029087:23809-23828 | Msa1440450:CDS | 35.0% | |
| GCTAGAAATGAAACAAGATG+CGG | - | tig0029087:23918-23937 | Msa1440450:CDS | 35.0% | |
| ATTCGGAGTAATAACAACGT+TGG | - | tig0029087:24095-24114 | Msa1440450:CDS | 35.0% | |
| !! | TGTTTTAAGCAGCCATGATG+CGG | - | tig0029087:23554-23573 | Msa1440450:CDS | 40.0% |
| ! | GAATGAGTTGGACGAAGAAT+TGG | - | tig0029087:23581-23600 | Msa1440450:CDS | 40.0% |
| GAATTGGAAGGTTTAAGCCA+TGG | - | tig0029087:23597-23616 | Msa1440450:CDS | 40.0% | |
| GAAGGTTTAAGCCATGGAAT+TGG | - | tig0029087:23603-23622 | Msa1440450:CDS | 40.0% | |
| ! | ATTGGTTCCAAATCACCTTC+GGG | + | tig0029087:23639-23658 | None:intergenic | 40.0% |
| GGGAGGCAATCTATGTTTAT+TGG | + | tig0029087:23657-23676 | None:intergenic | 40.0% | |
| TTTCCTTCGAAGAAGTTGAG+AGG | - | tig0029087:23706-23725 | Msa1440450:CDS | 40.0% | |
| AAACCAGTAGTAGAACTGGT+AGG | + | tig0029087:23757-23776 | None:intergenic | 40.0% | |
| ! | TACCAGTTCTACTACTGGTT+TGG | - | tig0029087:23756-23775 | Msa1440450:CDS | 40.0% |
| !! | ACCAGTTCTACTACTGGTTT+GGG | - | tig0029087:23757-23776 | Msa1440450:CDS | 40.0% |
| !! | TACTGGTTTGGGAGAAGAAA+AGG | - | tig0029087:23768-23787 | Msa1440450:CDS | 40.0% |
| CTGATGATAAGGAATCGTAC+GGG | - | tig0029087:23810-23829 | Msa1440450:CDS | 40.0% | |
| GTGAACACACATAGGTTTGT+TGG | + | tig0029087:23845-23864 | None:intergenic | 40.0% | |
| ATTGCACCGTACATCTGATA+TGG | + | tig0029087:24128-24147 | None:intergenic | 40.0% | |
| ATATCAGATGTACGGTGCAA+TGG | - | tig0029087:24127-24146 | Msa1440450:CDS | 40.0% | |
| !! | GTTTTAAGCAGCCATGATGC+GGG | - | tig0029087:23555-23574 | Msa1440450:CDS | 45.0% |
| GAGTTGGACGAAGAATTGGA+AGG | - | tig0029087:23585-23604 | Msa1440450:CDS | 45.0% | |
| ! | GGGAGTTGTTTCCAATTCCA+TGG | + | tig0029087:23617-23636 | None:intergenic | 45.0% |
| TTGGTTCCAAATCACCTTCG+GGG | + | tig0029087:23638-23657 | None:intergenic | 45.0% | |
| GTACCTCTCAACTTCTTCGA+AGG | + | tig0029087:23712-23731 | None:intergenic | 45.0% | |
| ! | GAAGTTGAGAGGTACAGCTT+TGG | - | tig0029087:23717-23736 | Msa1440450:CDS | 45.0% |
| TCCCAAACCAGTAGTAGAAC+TGG | + | tig0029087:23761-23780 | None:intergenic | 45.0% | |
| GCAGGATTTAGAGAAGATCG+TGG | - | tig0029087:23867-23886 | Msa1440450:CDS | 45.0% | |
| ! | GTGGATTAACACCCGAGAAA+AGG | - | tig0029087:23886-23905 | Msa1440450:CDS | 45.0% |
| ! | AGCACGACGTTTCCTTTTCT+CGG | + | tig0029087:23901-23920 | None:intergenic | 45.0% |
| TACATGTCGACATTCACACC+AGG | - | tig0029087:23961-23980 | Msa1440450:CDS | 45.0% | |
| TCATTCCATCCAACAGCACA+TGG | + | tig0029087:24022-24041 | None:intergenic | 45.0% | |
| CTGTTGGATGGAATGATGTG+CGG | - | tig0029087:24026-24045 | Msa1440450:CDS | 45.0% | |
| ACTCATTCTCTCCCGCATCA+TGG | + | tig0029087:23569-23588 | None:intergenic | 50.0% | |
| TGATGCGGGAGAGAATGAGT+TGG | - | tig0029087:23569-23588 | Msa1440450:CDS | 50.0% | |
| ATTGGAAACAACTCCCCCGA+AGG | - | tig0029087:23621-23640 | Msa1440450:CDS | 50.0% | |
| TGGTTCCAAATCACCTTCGG+GGG | + | tig0029087:23637-23656 | None:intergenic | 50.0% | |
| CTGCCTACCAGTTCTACTAC+TGG | - | tig0029087:23751-23770 | Msa1440450:CDS | 50.0% | |
| ACCTATGTGTGTTCACGTGC+AGG | - | tig0029087:23849-23868 | Msa1440450:CDS | 50.0% | |
| TCCTGCACGTGAACACACAT+AGG | + | tig0029087:23853-23872 | None:intergenic | 50.0% | |
| ! | GCACGACGTTTCCTTTTCTC+GGG | + | tig0029087:23900-23919 | None:intergenic | 50.0% |
| AACTGTGACATACCATCGCC+TGG | + | tig0029087:23982-24001 | None:intergenic | 50.0% | |
| TGGTCACAACCATGTGCTGT+TGG | - | tig0029087:24010-24029 | Msa1440450:CDS | 50.0% | |
| ! | CACAACCATGTGCTGTTGGA+TGG | - | tig0029087:24014-24033 | Msa1440450:CDS | 50.0% |
| GGTGCAATGGCAAATGCTTC+TGG | - | tig0029087:24140-24159 | Msa1440450:CDS | 50.0% | |
| GCAATGGCAAATGCTTCTGG+TGG | - | tig0029087:24143-24162 | Msa1440450:intron | 50.0% | |
| CAACTCCCCCGAAGGTGATT+TGG | - | tig0029087:23629-23648 | Msa1440450:CDS | 55.0% | |
| TCGACATTCACACCAGGCGA+TGG | - | tig0029087:23967-23986 | Msa1440450:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0029087 | gene | 23550 | 24175 | 23550 | ID=Msa1440450;Name=Msa1440450 |
| tig0029087 | mRNA | 23550 | 24175 | 23550 | ID=Msa1440450-mRNA-1;Parent=Msa1440450;Name=Msa1440450-mRNA-1;_AED=0.13;_eAED=0.14;_QI=0|0|0|1|1|1|2|0|204 |
| tig0029087 | exon | 24171 | 24175 | 24171 | ID=Msa1440450-mRNA-1:exon:12264;Parent=Msa1440450-mRNA-1 |
| tig0029087 | exon | 23550 | 24159 | 23550 | ID=Msa1440450-mRNA-1:exon:12263;Parent=Msa1440450-mRNA-1 |
| tig0029087 | CDS | 24171 | 24175 | 24171 | ID=Msa1440450-mRNA-1:cds;Parent=Msa1440450-mRNA-1 |
| tig0029087 | CDS | 23550 | 24159 | 23550 | ID=Msa1440450-mRNA-1:cds;Parent=Msa1440450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
