Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1441990 | A0A072TPV3 | 87.363 | 182 | 15 | 1 | 1 | 182 | 1 | 174 | 3.53e-111 | 323 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1441990 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1441990 | MtrunA17_Chr8g0358731 | 87.363 | 182 | 15 | 1 | 1 | 182 | 1 | 174 | 6.79e-115 | 323 |
| Msa1441990 | MtrunA17_Chr8g0337271 | 34.872 | 195 | 100 | 6 | 1 | 168 | 1 | 195 | 7.21e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTATTCGCCAGTACATTTA+TGG | 0.169458 | tig0029550:-48177 | Msa1441990:CDS |
| CAAAGTGCTCTTAGAAGTTT+AGG | 0.233419 | tig0029550:-48356 | Msa1441990:CDS |
| TCGGATCTTGCATATTCATT+TGG | 0.326460 | tig0029550:+47833 | None:intergenic |
| ACGCCAAGATGGTCGTCAAT+TGG | 0.390051 | tig0029550:-48237 | Msa1441990:CDS |
| TGCTGCACCTTGTCTGTCAT+AGG | 0.406280 | tig0029550:+48295 | None:intergenic |
| TGCAATAATGATTGTGAATA+CGG | 0.437294 | tig0029550:-48413 | Msa1441990:CDS |
| ATTCCAACCGCTTGTGAAAG+TGG | 0.469159 | tig0029550:-48062 | Msa1441990:CDS |
| CTTGTAACTACGAGACATGA+TGG | 0.480223 | tig0029550:+48118 | None:intergenic |
| CATCTTGTAAATTTGCATGA+AGG | 0.483536 | tig0029550:+48091 | None:intergenic |
| GATCTTGCATATTCATTTGG+AGG | 0.493998 | tig0029550:+47836 | None:intergenic |
| TCTCCAATTGACGACCATCT+TGG | 0.509670 | tig0029550:+48234 | None:intergenic |
| TCTCCACTTTCACAAGCGGT+TGG | 0.513951 | tig0029550:+48059 | None:intergenic |
| ATCTAACATCATGAGTATGA+TGG | 0.517001 | tig0029550:-48330 | Msa1441990:CDS |
| GGTATACAGCAATGCATCCA+AGG | 0.519872 | tig0029550:-47918 | Msa1441990:intron |
| AGATGGTCGTCAATTGGAGA+TGG | 0.520285 | tig0029550:-48231 | Msa1441990:CDS |
| ACTAACCACAATCTACTCTT+CGG | 0.522583 | tig0029550:+47814 | None:intergenic |
| TGAAAATCTTCATTTCTATG+TGG | 0.529192 | tig0029550:+48377 | None:intergenic |
| AATGACGACTTGCTTACCCT+TGG | 0.530306 | tig0029550:+47901 | None:intergenic |
| ATCTTGCATATTCATTTGGA+GGG | 0.532675 | tig0029550:+47837 | None:intergenic |
| ACAAAAGGCCATAAATGTAC+TGG | 0.537910 | tig0029550:+48169 | None:intergenic |
| CGAAGGAAACAAACATGCTA+GGG | 0.537931 | tig0029550:-48468 | Msa1441990:CDS |
| CTTAGCATAATACCTACAAA+AGG | 0.549427 | tig0029550:+48154 | None:intergenic |
| TTGTAACTACGAGACATGAT+GGG | 0.552157 | tig0029550:+48119 | None:intergenic |
| ACGAAGGAAACAAACATGCT+AGG | 0.563976 | tig0029550:-48469 | Msa1441990:CDS |
| TTCTGAAGAAAGTGATGACA+TGG | 0.568922 | tig0029550:-47867 | Msa1441990:CDS |
| GCATGGCAACGACGCCAAGA+TGG | 0.574695 | tig0029550:-48248 | Msa1441990:CDS |
| AAGAGATATCTTCCTTTGAG+AGG | 0.575332 | tig0029550:-48205 | Msa1441990:CDS |
| TTCTCAGTCAAGGTCGAGCA+TGG | 0.575982 | tig0029550:-48265 | Msa1441990:CDS |
| GGATTCTCCACTTTCACAAG+CGG | 0.586954 | tig0029550:+48055 | None:intergenic |
| CATGTCTCGTAGTTACAAGA+TGG | 0.593567 | tig0029550:-48114 | Msa1441990:CDS |
| GATGGTCGTCAATTGGAGAT+GGG | 0.600387 | tig0029550:-48230 | Msa1441990:CDS |
| CTACGAGACATGATGGGGAA+TGG | 0.605683 | tig0029550:+48125 | None:intergenic |
| GAATAGTGCTTTCCTCTCAA+AGG | 0.618448 | tig0029550:+48193 | None:intergenic |
| GCAAACTCGATTCTCAGTCA+AGG | 0.622555 | tig0029550:-48275 | Msa1441990:CDS |
| CTTGTGAAAGTGGAGAATCC+AGG | 0.624797 | tig0029550:-48052 | Msa1441990:intron |
| CATGAGTATGATGGAAAGTG+TGG | 0.625793 | tig0029550:-48321 | Msa1441990:CDS |
| GTATACAGCAATGCATCCAA+GGG | 0.652464 | tig0029550:-47917 | Msa1441990:intron |
| GTATATCCATACATGAACGA+AGG | 0.669151 | tig0029550:-48485 | None:intergenic |
| TGTAACTACGAGACATGATG+GGG | 0.692794 | tig0029550:+48120 | None:intergenic |
| GTGGAAACCTATGACAGACA+AGG | 0.716652 | tig0029550:-48302 | Msa1441990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACACTCTTTATATCAAATTT+TGG | - | tig0029550:48360-48379 | Msa1441990:CDS | 20.0% |
| ! | TGCAATAATGATTGTGAATA+CGG | - | tig0029550:47886-47905 | Msa1441990:CDS | 25.0% |
| ! | TGAAAATCTTCATTTCTATG+TGG | + | tig0029550:47925-47944 | None:intergenic | 25.0% |
| !! | TGATATAAAGAGTGTGTTAT+AGG | + | tig0029550:48355-48374 | None:intergenic | 25.0% |
| ATCTAACATCATGAGTATGA+TGG | - | tig0029550:47969-47988 | Msa1441990:intron | 30.0% | |
| CTTAGCATAATACCTACAAA+AGG | + | tig0029550:48148-48167 | None:intergenic | 30.0% | |
| CATCTTGTAAATTTGCATGA+AGG | + | tig0029550:48211-48230 | None:intergenic | 30.0% | |
| AGTTCAATATAGAGATTACC+TGG | + | tig0029550:48268-48287 | None:intergenic | 30.0% | |
| ATCTTGCATATTCATTTGGA+GGG | + | tig0029550:48465-48484 | None:intergenic | 30.0% | |
| ! | CAAAGTGCTCTTAGAAGTTT+AGG | - | tig0029550:47943-47962 | Msa1441990:intron | 35.0% |
| AAGAGATATCTTCCTTTGAG+AGG | - | tig0029550:48094-48113 | Msa1441990:CDS | 35.0% | |
| ACTATTCGCCAGTACATTTA+TGG | - | tig0029550:48122-48141 | Msa1441990:CDS | 35.0% | |
| ACAAAAGGCCATAAATGTAC+TGG | + | tig0029550:48133-48152 | None:intergenic | 35.0% | |
| ! | GTACATTTATGGCCTTTTGT+AGG | - | tig0029550:48133-48152 | Msa1441990:CDS | 35.0% |
| TTGTAACTACGAGACATGAT+GGG | + | tig0029550:48183-48202 | None:intergenic | 35.0% | |
| ! | TTCTGAAGAAAGTGATGACA+TGG | - | tig0029550:48432-48451 | Msa1441990:CDS | 35.0% |
| GATCTTGCATATTCATTTGG+AGG | + | tig0029550:48466-48485 | None:intergenic | 35.0% | |
| TCGGATCTTGCATATTCATT+TGG | + | tig0029550:48469-48488 | None:intergenic | 35.0% | |
| ACGAAGGAAACAAACATGCT+AGG | - | tig0029550:47830-47849 | Msa1441990:CDS | 40.0% | |
| CGAAGGAAACAAACATGCTA+GGG | - | tig0029550:47831-47850 | Msa1441990:CDS | 40.0% | |
| ! | CATGAGTATGATGGAAAGTG+TGG | - | tig0029550:47978-47997 | Msa1441990:intron | 40.0% |
| GAATAGTGCTTTCCTCTCAA+AGG | + | tig0029550:48109-48128 | None:intergenic | 40.0% | |
| TGTAACTACGAGACATGATG+GGG | + | tig0029550:48182-48201 | None:intergenic | 40.0% | |
| CTTGTAACTACGAGACATGA+TGG | + | tig0029550:48184-48203 | None:intergenic | 40.0% | |
| CATGTCTCGTAGTTACAAGA+TGG | - | tig0029550:48185-48204 | Msa1441990:CDS | 40.0% | |
| GTATACAGCAATGCATCCAA+GGG | - | tig0029550:48382-48401 | Msa1441990:CDS | 40.0% | |
| GTGGAAACCTATGACAGACA+AGG | - | tig0029550:47997-48016 | Msa1441990:intron | 45.0% | |
| GCAAACTCGATTCTCAGTCA+AGG | - | tig0029550:48024-48043 | Msa1441990:intron | 45.0% | |
| TCTCCAATTGACGACCATCT+TGG | + | tig0029550:48068-48087 | None:intergenic | 45.0% | |
| AGATGGTCGTCAATTGGAGA+TGG | - | tig0029550:48068-48087 | Msa1441990:CDS | 45.0% | |
| GATGGTCGTCAATTGGAGAT+GGG | - | tig0029550:48069-48088 | Msa1441990:CDS | 45.0% | |
| ATTCCAACCGCTTGTGAAAG+TGG | - | tig0029550:48237-48256 | Msa1441990:CDS | 45.0% | |
| GGATTCTCCACTTTCACAAG+CGG | + | tig0029550:48247-48266 | None:intergenic | 45.0% | |
| CTTGTGAAAGTGGAGAATCC+AGG | - | tig0029550:48247-48266 | Msa1441990:CDS | 45.0% | |
| GGTATACAGCAATGCATCCA+AGG | - | tig0029550:48381-48400 | Msa1441990:CDS | 45.0% | |
| ! | AATGACGACTTGCTTACCCT+TGG | + | tig0029550:48401-48420 | None:intergenic | 45.0% |
| TGCTGCACCTTGTCTGTCAT+AGG | + | tig0029550:48007-48026 | None:intergenic | 50.0% | |
| TTCTCAGTCAAGGTCGAGCA+TGG | - | tig0029550:48034-48053 | Msa1441990:intron | 50.0% | |
| ACGCCAAGATGGTCGTCAAT+TGG | - | tig0029550:48062-48081 | Msa1441990:CDS | 50.0% | |
| ! | CTACGAGACATGATGGGGAA+TGG | + | tig0029550:48177-48196 | None:intergenic | 50.0% |
| TCTCCACTTTCACAAGCGGT+TGG | + | tig0029550:48243-48262 | None:intergenic | 50.0% | |
| GCATGGCAACGACGCCAAGA+TGG | - | tig0029550:48051-48070 | Msa1441990:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0029550 | gene | 47826 | 48495 | 47826 | ID=Msa1441990;Name=Msa1441990 |
| tig0029550 | mRNA | 47826 | 48495 | 47826 | ID=Msa1441990-mRNA-1;Parent=Msa1441990;Name=Msa1441990-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0|1|1|1|2|0|182 |
| tig0029550 | exon | 48053 | 48495 | 48053 | ID=Msa1441990-mRNA-1:exon:13119;Parent=Msa1441990-mRNA-1 |
| tig0029550 | exon | 47826 | 47931 | 47826 | ID=Msa1441990-mRNA-1:exon:13118;Parent=Msa1441990-mRNA-1 |
| tig0029550 | CDS | 48053 | 48495 | 48053 | ID=Msa1441990-mRNA-1:cds;Parent=Msa1441990-mRNA-1 |
| tig0029550 | CDS | 47826 | 47931 | 47826 | ID=Msa1441990-mRNA-1:cds;Parent=Msa1441990-mRNA-1 |
| Gene Sequence |
| Protein sequence |
