AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1443660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443660 MtrunA17_Chr1g0198651 97.251 291 8 0 1 291 1 291 0.0 598
Msa1443660 MtrunA17_Chr5g0422571 62.633 281 90 5 1 275 1 272 1.54e-113 329
Msa1443660 MtrunA17_Chr1g0173841 65.094 212 60 2 75 286 188 385 1.91e-99 297
Msa1443660 MtrunA17_Chr7g0258011 71.809 188 39 2 99 286 173 346 2.03e-99 295
Msa1443660 MtrunA17_Chr2g0328921 71.429 189 40 3 99 286 135 310 1.76e-96 286
Msa1443660 MtrunA17_Chr8g0352011 69.730 185 42 2 99 283 163 333 2.74e-96 287
Msa1443660 MtrunA17_Chr2g0321291 69.841 189 41 4 99 284 130 305 7.18e-96 285
Msa1443660 MtrunA17_Chr4g0026521 68.254 189 45 4 99 284 115 291 1.96e-95 283
Msa1443660 MtrunA17_Chr4g0053341 68.391 174 50 2 87 260 61 229 1.18e-86 259
Msa1443660 MtrunA17_Chr1g0200421 41.447 152 83 6 107 255 244 392 5.71e-27 109
Msa1443660 MtrunA17_Chr2g0320341 38.415 164 92 8 105 263 235 394 1.97e-23 99.0
Msa1443660 MtrunA17_Chr1g0181381 31.081 222 135 7 60 276 8 216 2.23e-21 94.0
Msa1443660 MtrunA17_Chr3g0123941 35.000 180 97 8 117 283 139 311 3.56e-21 91.7
Msa1443660 MtrunA17_Chr7g0263941 35.862 145 78 5 107 249 55 186 4.36e-21 92.4
Msa1443660 MtrunA17_Chr8g0382121 35.542 166 99 6 117 279 127 287 7.86e-21 90.5
Msa1443660 MtrunA17_Chr1g0163001 31.188 202 124 5 50 249 7 195 1.02e-20 92.0
Msa1443660 MtrunA17_Chr1g0210821 35.862 145 78 5 107 249 65 196 1.50e-20 91.7
Msa1443660 MtrunA17_Chr4g0029521 34.507 142 81 4 109 249 61 191 1.71e-20 91.3
Msa1443660 MtrunA17_Chr1g0185121 34.667 150 84 5 107 254 61 198 3.04e-20 90.1
Msa1443660 MtrunA17_Chr1g0199101 35.172 145 79 5 107 249 68 199 3.49e-20 90.5
Msa1443660 MtrunA17_Chr8g0342421 33.333 156 89 6 109 262 85 227 4.69e-20 90.1
Msa1443660 MtrunA17_Chr1g0162971 37.063 143 75 5 109 249 64 193 5.18e-20 89.7
Msa1443660 MtrunA17_Chr1g0151351 34.161 161 91 6 91 249 47 194 5.72e-20 89.4
Msa1443660 MtrunA17_Chr7g0268961 35.664 143 77 6 109 249 45 174 8.73e-20 89.0
Msa1443660 MtrunA17_Chr2g0326381 35.211 142 80 4 109 249 55 185 1.03e-19 88.6
Msa1443660 MtrunA17_Chr1g0191991 33.520 179 90 8 103 273 74 231 1.21e-19 89.0
Msa1443660 MtrunA17_Chr2g0330171 35.172 145 79 6 107 249 67 198 3.75e-19 87.0
Msa1443660 MtrunA17_Chr8g0373541 31.188 202 117 7 85 279 29 215 5.52e-19 86.7
Msa1443660 MtrunA17_Chr4g0040571 33.553 152 88 5 107 257 60 199 3.26e-18 84.3
Msa1443660 MtrunA17_Chr3g0141431 30.405 148 89 5 107 252 43 178 7.19e-17 80.1
Msa1443660 MtrunA17_Chr2g0324991 35.461 141 76 7 107 244 13 141 2.12e-16 74.7
Msa1443660 MtrunA17_Chr8g0375521 27.083 192 124 6 61 248 2 181 3.72e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443660 AT1G13290.1 65.447 246 61 5 23 258 12 243 5.62e-108 316
Msa1443660 AT3G57670.1 71.429 196 40 4 99 292 201 382 5.35e-98 293
Msa1443660 AT1G51220.1 75.152 165 38 1 100 264 163 324 5.89e-95 284
Msa1443660 AT3G20880.1 77.987 159 32 1 97 255 238 393 5.78e-94 284
Msa1443660 AT1G08290.1 73.810 168 38 3 99 265 164 326 4.23e-93 279
Msa1443660 AT1G34790.1 75.159 157 36 1 100 256 130 283 7.47e-88 265
Msa1443660 AT1G34370.3 41.176 153 84 5 107 255 27 177 3.13e-26 105
Msa1443660 AT1G34370.2 41.176 153 84 5 107 255 237 387 1.28e-25 106
Msa1443660 AT1G34370.1 41.176 153 84 5 107 255 237 387 1.28e-25 106
Msa1443660 AT5G22890.1 40.645 155 84 7 106 255 209 360 1.50e-24 102
Msa1443660 AT5G03150.1 35.211 142 80 4 109 249 76 206 5.34e-21 93.2
Msa1443660 AT1G55110.3 33.758 157 90 5 107 261 84 228 6.94e-21 92.4
Msa1443660 AT1G55110.1 33.758 157 90 5 107 261 84 228 6.94e-21 92.4
Msa1443660 AT1G55110.2 33.758 157 90 5 107 261 84 228 6.94e-21 92.4
Msa1443660 AT5G44160.1 32.178 202 106 8 63 257 20 197 2.26e-20 91.3
Msa1443660 AT2G02070.2 35.443 158 87 5 94 249 60 204 3.64e-20 90.9
Msa1443660 AT2G02070.1 35.443 158 87 5 94 249 60 204 3.64e-20 90.9
Msa1443660 AT2G02080.1 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa1443660 AT2G02080.4 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa1443660 AT2G02080.5 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa1443660 AT2G02080.3 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa1443660 AT3G45260.2 34.615 156 90 4 109 263 62 206 4.20e-20 90.1
Msa1443660 AT3G45260.1 34.615 156 90 4 109 263 62 206 4.20e-20 90.1
Msa1443660 AT5G60470.1 35.211 142 80 4 109 249 58 188 5.76e-20 89.7
Msa1443660 AT5G60470.3 35.211 142 80 4 109 249 63 193 7.00e-20 89.7
Msa1443660 AT5G60470.2 35.211 142 80 4 109 249 67 197 7.05e-20 89.7
Msa1443660 AT5G66730.1 34.000 150 84 5 107 254 53 189 1.07e-19 89.4
Msa1443660 AT1G14580.1 37.241 145 76 6 107 249 74 205 1.38e-19 89.0
Msa1443660 AT1G14580.2 37.241 145 76 6 107 249 74 205 1.38e-19 89.0
Msa1443660 AT1G14580.3 37.241 145 76 6 107 249 88 219 1.57e-19 88.6
Msa1443660 AT1G03840.2 31.638 177 107 6 83 257 35 199 2.82e-19 88.2
Msa1443660 AT2G02080.6 36.620 142 75 5 110 249 1 129 3.44e-19 87.4
Msa1443660 AT2G02080.2 36.620 142 75 5 110 249 1 129 3.44e-19 87.4
Msa1443660 AT3G50700.1 35.172 145 79 5 107 249 55 186 5.48e-19 87.0
Msa1443660 AT5G44160.2 34.667 150 85 5 109 257 21 158 1.07e-18 85.9
Msa1443660 AT1G03840.1 31.844 179 106 6 83 257 35 201 1.67e-18 85.9
Msa1443660 AT3G13810.4 32.051 156 94 4 107 261 91 235 2.39e-18 85.5
Msa1443660 AT3G13810.1 32.051 156 94 4 107 261 91 235 2.39e-18 85.5
Msa1443660 AT3G13810.5 32.051 156 94 4 107 261 86 230 2.44e-18 85.5
Msa1443660 AT3G13810.2 32.895 152 90 4 107 257 92 232 2.54e-18 85.1
Msa1443660 AT3G13810.6 32.051 156 94 4 107 261 72 216 2.65e-18 85.1
Msa1443660 AT3G13810.3 32.895 152 90 4 107 257 78 218 2.66e-18 85.1
Msa1443660 AT4G02670.1 34.028 144 83 4 107 249 74 206 4.20e-18 84.3
Msa1443660 AT4G02670.2 34.028 144 83 4 107 249 76 208 4.20e-18 84.3

Find 81 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCGAGTTTGGGAGGTATT+TGG 0.247423 tig0030496:+6968 None:intergenic
CTCCCATTATTCCTCAATAT+TGG 0.256317 tig0030496:-6795 Msa1443660:CDS
AGTGAAAGTTGGATTAATTA+AGG 0.285981 tig0030496:+6834 None:intergenic
GATCCAATATTGAGGAATAA+TGG 0.295991 tig0030496:+6792 None:intergenic
AAAGGACCGGAGTCTCTAAA+AGG 0.306782 tig0030496:-6397 Msa1443660:CDS
GATGAAGATGATGCTGGTTT+TGG 0.319383 tig0030496:+6373 None:intergenic
TGCAGACAGTGCAAGAAAAC+TGG 0.325433 tig0030496:+6737 None:intergenic
TCTCCTGCTCAAATCCTTGT+TGG 0.331954 tig0030496:-6766 Msa1443660:CDS
TTCCTCTCTCTTGCTCCTCC+TGG 0.347551 tig0030496:-6994 Msa1443660:CDS
AAAGATCACGTTCGTGCGTT+TGG 0.349116 tig0030496:-6097 Msa1443660:CDS
AGAAGAATTGTGGGAAGCTT+TGG 0.351421 tig0030496:-6162 Msa1443660:CDS
AATGGGAGTGTGAGATGGTT+TGG 0.355414 tig0030496:+6810 None:intergenic
AGGGATAGTAGTAGTAATAG+TGG 0.376112 tig0030496:+6936 None:intergenic
AGAGAGGTAGTAGTAGAAAT+AGG 0.389319 tig0030496:+6898 None:intergenic
ATCCAATATTGAGGAATAAT+GGG 0.396877 tig0030496:+6793 None:intergenic
CACGTTCGTGCGTTTGGTAA+TGG 0.397696 tig0030496:-6091 Msa1443660:CDS
CTACATTTACAGCACAAGTA+TGG 0.418258 tig0030496:+6866 None:intergenic
GATGGTTTGGAAGTGAAAGT+TGG 0.427167 tig0030496:+6823 None:intergenic
TCTTTCAGTGGTCTTGCCCT+TGG 0.430630 tig0030496:+6292 None:intergenic
TAGTAGTAATAGTGGGATGA+GGG 0.441652 tig0030496:+6944 None:intergenic
CATTTGCTGTGAGAGGAGAT+TGG 0.445706 tig0030496:-6195 Msa1443660:CDS
TTGGGAGGTATTTGGTGGCC+AGG 0.460715 tig0030496:+6976 None:intergenic
GGGGACATGGATCTCAATAT+AGG 0.462797 tig0030496:-6420 Msa1443660:CDS
GTAGTAGTAATAGTGGGATG+AGG 0.468089 tig0030496:+6943 None:intergenic
GAAATAGGTATTTGATGCAA+GGG 0.489738 tig0030496:+6913 None:intergenic
CATGTTGTTGAAACGGTTGA+AGG 0.493614 tig0030496:+6708 None:intergenic
TACATGGAAGAAGAAGAAAC+TGG 0.497808 tig0030496:-6010 Msa1443660:CDS
AGGAGCAAGAGAGAGGAAGA+TGG 0.499455 tig0030496:+6999 None:intergenic
GGGATAGTAGTAGTAATAGT+GGG 0.503268 tig0030496:+6937 None:intergenic
CATTCGAGTGTAGGAAGTGT+AGG 0.505760 tig0030496:-6222 Msa1443660:CDS
CGAGTTTGGGAGGTATTTGG+TGG 0.508108 tig0030496:+6971 None:intergenic
CCATGTTACTGTTGTGCTGA+GGG 0.513723 tig0030496:-6337 Msa1443660:CDS
AGAAATAGGTATTTGATGCA+AGG 0.514616 tig0030496:+6912 None:intergenic
GGACACATGAGAAGAATTGT+GGG 0.524360 tig0030496:-6171 Msa1443660:CDS
ACAAGGATTTGAGCAGGAGA+AGG 0.531708 tig0030496:+6769 None:intergenic
CAACCGTTTCAACAACATGC+AGG 0.536317 tig0030496:-6704 Msa1443660:intron
ATTCCAACGTGTGGGGAGCA+TGG 0.539289 tig0030496:+6068 None:intergenic
GAAACAACATAAACTATCCA+AGG 0.546681 tig0030496:-6309 Msa1443660:CDS
GAACCTGCATGTTGTTGAAA+CGG 0.548476 tig0030496:+6701 None:intergenic
AACTCAAAGATTCCAACGTG+TGG 0.551951 tig0030496:+6059 None:intergenic
AGGACACATGAGAAGAATTG+TGG 0.552345 tig0030496:-6172 Msa1443660:CDS
AGCATTGATGAAGATGATGC+TGG 0.557005 tig0030496:+6367 None:intergenic
AAGGACCGGAGTCTCTAAAA+GGG 0.557565 tig0030496:-6396 Msa1443660:CDS
ATCTAAGCTGCTGCTGCAAG+AGG 0.562751 tig0030496:+7032 None:intergenic
TTGTATGGTAGATGCATATG+TGG 0.564484 tig0030496:-6441 Msa1443660:intron
GGAGGTATTTGGTGGCCAGG+AGG 0.567766 tig0030496:+6979 None:intergenic
GAGGGAAGTCATTCGAGTGT+AGG 0.568390 tig0030496:-6231 Msa1443660:CDS
ATAGGTATTTGATGCAAGGG+AGG 0.571468 tig0030496:+6916 None:intergenic
GGACCAACAAGGATTTGAGC+AGG 0.574385 tig0030496:+6763 None:intergenic
ACTCAAAGATTCCAACGTGT+GGG 0.574474 tig0030496:+6060 None:intergenic
ACACACTACAAAAGAAAGCA+TGG 0.577637 tig0030496:-6253 Msa1443660:CDS
CAAGGATTTGAGCAGGAGAA+GGG 0.578543 tig0030496:+6770 None:intergenic
TAAAGTAAATGAAAATTACA+TGG 0.583660 tig0030496:-6026 Msa1443660:CDS
AGTAGTAATAGTGGGATGAG+GGG 0.585249 tig0030496:+6945 None:intergenic
GCAGACAGTGCAAGAAAACT+GGG 0.587578 tig0030496:+6738 None:intergenic
TTGCTGTGAGAGGAGATTGG+AGG 0.592461 tig0030496:-6192 Msa1443660:CDS
GGCCAGGAGGAGCAAGAGAG+AGG 0.593784 tig0030496:+6992 None:intergenic
GGAATAATGGGAGTGTGAGA+TGG 0.594723 tig0030496:+6805 None:intergenic
ATCTCAATATAGGAAAGGAC+CGG 0.597133 tig0030496:-6410 Msa1443660:CDS
AGGGTTCTGAAATCTTTCAG+TGG 0.606548 tig0030496:+6280 None:intergenic
GGAGAAGGGATCCAATATTG+AGG 0.614687 tig0030496:+6784 None:intergenic
ACCATGTTACTGTTGTGCTG+AGG 0.615026 tig0030496:-6338 Msa1443660:CDS
CATGGATCTCAATATAGGAA+AGG 0.616598 tig0030496:-6415 Msa1443660:CDS
TGTATGGTAGATGCATATGT+GGG 0.620648 tig0030496:-6440 Msa1443660:intron
AGGAAGTCATTTGCTGTGAG+AGG 0.625916 tig0030496:-6202 Msa1443660:CDS
CCCTCAGCACAACAGTAACA+TGG 0.627644 tig0030496:+6337 None:intergenic
CTCAAAGATTCCAACGTGTG+GGG 0.627798 tig0030496:+6061 None:intergenic
GTAGTAATAGTGGGATGAGG+GGG 0.632310 tig0030496:+6946 None:intergenic
TAGATGCATATGTGGGGACA+TGG 0.632523 tig0030496:-6433 Msa1443660:CDS
TCTAAGCTGCTGCTGCAAGA+GGG 0.633302 tig0030496:+7033 None:intergenic
AGTATGGTCATGGTGAAGAG+AGG 0.640967 tig0030496:+6882 None:intergenic
TAGGTATTTGATGCAAGGGA+GGG 0.641902 tig0030496:+6917 None:intergenic
ACTACAAAAGAAAGCATGGA+GGG 0.646182 tig0030496:-6249 Msa1443660:CDS
TGGCCATGCTCCCCACACGT+TGG 0.648703 tig0030496:-6071 Msa1443660:CDS
AAACAACATAAACTATCCAA+GGG 0.649476 tig0030496:-6308 Msa1443660:CDS
TTACAGCACAAGTATGGTCA+TGG 0.652632 tig0030496:+6872 None:intergenic
ACAGTGCAAGAAAACTGGGT+AGG 0.667615 tig0030496:+6742 None:intergenic
AAAACTGGGTAGGACCAACA+AGG 0.677453 tig0030496:+6752 None:intergenic
GTACTGATGATGAGTTTGCA+TGG 0.690956 tig0030496:+7077 None:intergenic
CACTACAAAAGAAAGCATGG+AGG 0.735997 tig0030496:-6250 Msa1443660:CDS
GTATGGTAGATGCATATGTG+GGG 0.763722 tig0030496:-6439 Msa1443660:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGGATAAAAATATAATAA+TGG + tig0030496:6363-6382 None:intergenic 10.0%
!! AAAGGATAAAAATATAATAA+TGG + tig0030496:6363-6382 None:intergenic 10.0%
!! TCATGATATAGTAAAAATAA+AGG + tig0030496:6381-6400 None:intergenic 15.0%
!! CTAATGAATGATTAATTTAA+AGG + tig0030496:6452-6471 None:intergenic 15.0%
!! AAACATGATTCATATATATA+TGG - tig0030496:6550-6569 Msa1443660:intron 15.0%
!! TAAAGTAAATGAAAATTACA+TGG - tig0030496:7010-7029 Msa1443660:CDS 15.0%
!! TCATGATATAGTAAAAATAA+AGG + tig0030496:6381-6400 None:intergenic 15.0%
!! CTAATGAATGATTAATTTAA+AGG + tig0030496:6452-6471 None:intergenic 15.0%
!! AAACATGATTCATATATATA+TGG - tig0030496:6550-6569 Msa1443660:intron 15.0%
!! TAAAGTAAATGAAAATTACA+TGG - tig0030496:7010-7029 Msa1443660:CDS 15.0%
!! TATGATATTGTTGTATTGTA+TGG - tig0030496:6580-6599 Msa1443660:intron 20.0%
!! TATGATATTGTTGTATTGTA+TGG - tig0030496:6580-6599 Msa1443660:intron 20.0%
! AGTGAAAGTTGGATTAATTA+AGG + tig0030496:6205-6224 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG + tig0030496:6246-6265 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG + tig0030496:6447-6466 None:intergenic 25.0%
! AAACAACATAAACTATCCAA+GGG - tig0030496:6728-6747 Msa1443660:CDS 25.0%
! AGTGAAAGTTGGATTAATTA+AGG + tig0030496:6205-6224 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG + tig0030496:6246-6265 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG + tig0030496:6447-6466 None:intergenic 25.0%
! AAACAACATAAACTATCCAA+GGG - tig0030496:6728-6747 Msa1443660:CDS 25.0%
GAAATAGGTATTTGATGCAA+GGG + tig0030496:6126-6145 None:intergenic 30.0%
AGAAATAGGTATTTGATGCA+AGG + tig0030496:6127-6146 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG + tig0030496:6247-6266 None:intergenic 30.0%
TCATTCATGGAAGAAAAGTA+AGG + tig0030496:6501-6520 None:intergenic 30.0%
AGCTCAATATGATTCATTCA+TGG + tig0030496:6514-6533 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG - tig0030496:6727-6746 Msa1443660:CDS 30.0%
! TCTTTTGTAGTGTGTTTGAA+GGG + tig0030496:6778-6797 None:intergenic 30.0%
! TTCTTTTGTAGTGTGTTTGA+AGG + tig0030496:6779-6798 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG - tig0030496:6984-7003 Msa1443660:CDS 30.0%
GAAATAGGTATTTGATGCAA+GGG + tig0030496:6126-6145 None:intergenic 30.0%
AGAAATAGGTATTTGATGCA+AGG + tig0030496:6127-6146 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG + tig0030496:6247-6266 None:intergenic 30.0%
TCATTCATGGAAGAAAAGTA+AGG + tig0030496:6501-6520 None:intergenic 30.0%
AGCTCAATATGATTCATTCA+TGG + tig0030496:6514-6533 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG - tig0030496:6727-6746 Msa1443660:CDS 30.0%
! TCTTTTGTAGTGTGTTTGAA+GGG + tig0030496:6778-6797 None:intergenic 30.0%
! TTCTTTTGTAGTGTGTTTGA+AGG + tig0030496:6779-6798 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG - tig0030496:6984-7003 Msa1443660:CDS 30.0%
! TAGTAGTAATAGTGGGATGA+GGG + tig0030496:6095-6114 None:intergenic 35.0%
GGGATAGTAGTAGTAATAGT+GGG + tig0030496:6102-6121 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG + tig0030496:6103-6122 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG + tig0030496:6141-6160 None:intergenic 35.0%
CTACATTTACAGCACAAGTA+TGG + tig0030496:6173-6192 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG - tig0030496:6241-6260 Msa1443660:CDS 35.0%
TTGTATGGTAGATGCATATG+TGG - tig0030496:6595-6614 Msa1443660:intron 35.0%
TGTATGGTAGATGCATATGT+GGG - tig0030496:6596-6615 Msa1443660:intron 35.0%
CATGGATCTCAATATAGGAA+AGG - tig0030496:6621-6640 Msa1443660:intron 35.0%
ATCTCAATATAGGAAAGGAC+CGG - tig0030496:6626-6645 Msa1443660:intron 35.0%
ACACACTACAAAAGAAAGCA+TGG - tig0030496:6783-6802 Msa1443660:CDS 35.0%
! ACTACAAAAGAAAGCATGGA+GGG - tig0030496:6787-6806 Msa1443660:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG - tig0030496:6888-6907 Msa1443660:CDS 35.0%
TACATGGAAGAAGAAGAAAC+TGG - tig0030496:7026-7045 Msa1443660:CDS 35.0%
! TAGTAGTAATAGTGGGATGA+GGG + tig0030496:6095-6114 None:intergenic 35.0%
GGGATAGTAGTAGTAATAGT+GGG + tig0030496:6102-6121 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG + tig0030496:6103-6122 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG + tig0030496:6141-6160 None:intergenic 35.0%
CTACATTTACAGCACAAGTA+TGG + tig0030496:6173-6192 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG - tig0030496:6241-6260 Msa1443660:CDS 35.0%
TTGTATGGTAGATGCATATG+TGG - tig0030496:6595-6614 Msa1443660:intron 35.0%
TGTATGGTAGATGCATATGT+GGG - tig0030496:6596-6615 Msa1443660:intron 35.0%
CATGGATCTCAATATAGGAA+AGG - tig0030496:6621-6640 Msa1443660:intron 35.0%
ATCTCAATATAGGAAAGGAC+CGG - tig0030496:6626-6645 Msa1443660:intron 35.0%
ACACACTACAAAAGAAAGCA+TGG - tig0030496:6783-6802 Msa1443660:CDS 35.0%
! ACTACAAAAGAAAGCATGGA+GGG - tig0030496:6787-6806 Msa1443660:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG - tig0030496:6888-6907 Msa1443660:CDS 35.0%
TACATGGAAGAAGAAGAAAC+TGG - tig0030496:7026-7045 Msa1443660:CDS 35.0%
! AGTAGTAATAGTGGGATGAG+GGG + tig0030496:6094-6113 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG + tig0030496:6096-6115 None:intergenic 40.0%
TAGGTATTTGATGCAAGGGA+GGG + tig0030496:6122-6141 None:intergenic 40.0%
ATAGGTATTTGATGCAAGGG+AGG + tig0030496:6123-6142 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG + tig0030496:6167-6186 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG + tig0030496:6216-6235 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG + tig0030496:6331-6350 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG + tig0030496:6338-6357 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG - tig0030496:6597-6616 Msa1443660:intron 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG + tig0030496:6666-6685 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG + tig0030496:6672-6691 None:intergenic 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG + tig0030496:6759-6778 None:intergenic 40.0%
! CACTACAAAAGAAAGCATGG+AGG - tig0030496:6786-6805 Msa1443660:CDS 40.0%
AGGACACATGAGAAGAATTG+TGG - tig0030496:6864-6883 Msa1443660:CDS 40.0%
GGACACATGAGAAGAATTGT+GGG - tig0030496:6865-6884 Msa1443660:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG - tig0030496:6874-6893 Msa1443660:CDS 40.0%
ACTCAAAGATTCCAACGTGT+GGG + tig0030496:6979-6998 None:intergenic 40.0%
AACTCAAAGATTCCAACGTG+TGG + tig0030496:6980-6999 None:intergenic 40.0%
! AGTAGTAATAGTGGGATGAG+GGG + tig0030496:6094-6113 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG + tig0030496:6096-6115 None:intergenic 40.0%
TAGGTATTTGATGCAAGGGA+GGG + tig0030496:6122-6141 None:intergenic 40.0%
ATAGGTATTTGATGCAAGGG+AGG + tig0030496:6123-6142 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG + tig0030496:6167-6186 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG + tig0030496:6216-6235 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG + tig0030496:6331-6350 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG + tig0030496:6338-6357 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG - tig0030496:6597-6616 Msa1443660:intron 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG + tig0030496:6666-6685 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG + tig0030496:6672-6691 None:intergenic 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG + tig0030496:6759-6778 None:intergenic 40.0%
! CACTACAAAAGAAAGCATGG+AGG - tig0030496:6786-6805 Msa1443660:CDS 40.0%
AGGACACATGAGAAGAATTG+TGG - tig0030496:6864-6883 Msa1443660:CDS 40.0%
GGACACATGAGAAGAATTGT+GGG - tig0030496:6865-6884 Msa1443660:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG - tig0030496:6874-6893 Msa1443660:CDS 40.0%
ACTCAAAGATTCCAACGTGT+GGG + tig0030496:6979-6998 None:intergenic 40.0%
AACTCAAAGATTCCAACGTG+TGG + tig0030496:6980-6999 None:intergenic 40.0%
! GTAGTAATAGTGGGATGAGG+GGG + tig0030496:6093-6112 None:intergenic 45.0%
AGTATGGTCATGGTGAAGAG+AGG + tig0030496:6157-6176 None:intergenic 45.0%
! AATGGGAGTGTGAGATGGTT+TGG + tig0030496:6229-6248 None:intergenic 45.0%
! GGAATAATGGGAGTGTGAGA+TGG + tig0030496:6234-6253 None:intergenic 45.0%
GGAGAAGGGATCCAATATTG+AGG + tig0030496:6255-6274 None:intergenic 45.0%
CAAGGATTTGAGCAGGAGAA+GGG + tig0030496:6269-6288 None:intergenic 45.0%
ACAAGGATTTGAGCAGGAGA+AGG + tig0030496:6270-6289 None:intergenic 45.0%
TCTCCTGCTCAAATCCTTGT+TGG - tig0030496:6270-6289 Msa1443660:CDS 45.0%
AAAACTGGGTAGGACCAACA+AGG + tig0030496:6287-6306 None:intergenic 45.0%
ACAGTGCAAGAAAACTGGGT+AGG + tig0030496:6297-6316 None:intergenic 45.0%
GCAGACAGTGCAAGAAAACT+GGG + tig0030496:6301-6320 None:intergenic 45.0%
TGCAGACAGTGCAAGAAAAC+TGG + tig0030496:6302-6321 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG - tig0030496:6332-6351 Msa1443660:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG - tig0030496:6603-6622 Msa1443660:intron 45.0%
GGGGACATGGATCTCAATAT+AGG - tig0030496:6616-6635 Msa1443660:intron 45.0%
AAAGGACCGGAGTCTCTAAA+AGG - tig0030496:6639-6658 Msa1443660:intron 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG - tig0030496:6640-6659 Msa1443660:intron 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG + tig0030496:6648-6667 None:intergenic 45.0%
ACCATGTTACTGTTGTGCTG+AGG - tig0030496:6698-6717 Msa1443660:intron 45.0%
CCATGTTACTGTTGTGCTGA+GGG - tig0030496:6699-6718 Msa1443660:intron 45.0%
! CATTCGAGTGTAGGAAGTGT+AGG - tig0030496:6814-6833 Msa1443660:CDS 45.0%
!! AGGAAGTCATTTGCTGTGAG+AGG - tig0030496:6834-6853 Msa1443660:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG - tig0030496:6841-6860 Msa1443660:CDS 45.0%
AAAGATCACGTTCGTGCGTT+TGG - tig0030496:6939-6958 Msa1443660:CDS 45.0%
CTCAAAGATTCCAACGTGTG+GGG + tig0030496:6978-6997 None:intergenic 45.0%
! GTAGTAATAGTGGGATGAGG+GGG + tig0030496:6093-6112 None:intergenic 45.0%
AGTATGGTCATGGTGAAGAG+AGG + tig0030496:6157-6176 None:intergenic 45.0%
! AATGGGAGTGTGAGATGGTT+TGG + tig0030496:6229-6248 None:intergenic 45.0%
! GGAATAATGGGAGTGTGAGA+TGG + tig0030496:6234-6253 None:intergenic 45.0%
GGAGAAGGGATCCAATATTG+AGG + tig0030496:6255-6274 None:intergenic 45.0%
CAAGGATTTGAGCAGGAGAA+GGG + tig0030496:6269-6288 None:intergenic 45.0%
ACAAGGATTTGAGCAGGAGA+AGG + tig0030496:6270-6289 None:intergenic 45.0%
TCTCCTGCTCAAATCCTTGT+TGG - tig0030496:6270-6289 Msa1443660:CDS 45.0%
AAAACTGGGTAGGACCAACA+AGG + tig0030496:6287-6306 None:intergenic 45.0%
ACAGTGCAAGAAAACTGGGT+AGG + tig0030496:6297-6316 None:intergenic 45.0%
GCAGACAGTGCAAGAAAACT+GGG + tig0030496:6301-6320 None:intergenic 45.0%
TGCAGACAGTGCAAGAAAAC+TGG + tig0030496:6302-6321 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG - tig0030496:6332-6351 Msa1443660:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG - tig0030496:6603-6622 Msa1443660:intron 45.0%
GGGGACATGGATCTCAATAT+AGG - tig0030496:6616-6635 Msa1443660:intron 45.0%
AAAGGACCGGAGTCTCTAAA+AGG - tig0030496:6639-6658 Msa1443660:intron 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG - tig0030496:6640-6659 Msa1443660:intron 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG + tig0030496:6648-6667 None:intergenic 45.0%
ACCATGTTACTGTTGTGCTG+AGG - tig0030496:6698-6717 Msa1443660:intron 45.0%
CCATGTTACTGTTGTGCTGA+GGG - tig0030496:6699-6718 Msa1443660:intron 45.0%
! CATTCGAGTGTAGGAAGTGT+AGG - tig0030496:6814-6833 Msa1443660:CDS 45.0%
!! AGGAAGTCATTTGCTGTGAG+AGG - tig0030496:6834-6853 Msa1443660:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG - tig0030496:6841-6860 Msa1443660:CDS 45.0%
AAAGATCACGTTCGTGCGTT+TGG - tig0030496:6939-6958 Msa1443660:CDS 45.0%
CTCAAAGATTCCAACGTGTG+GGG + tig0030496:6978-6997 None:intergenic 45.0%
TCTAAGCTGCTGCTGCAAGA+GGG + tig0030496:6006-6025 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG + tig0030496:6007-6026 None:intergenic 50.0%
AGGAGCAAGAGAGAGGAAGA+TGG + tig0030496:6040-6059 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG + tig0030496:6068-6087 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG + tig0030496:6071-6090 None:intergenic 50.0%
GGACCAACAAGGATTTGAGC+AGG + tig0030496:6276-6295 None:intergenic 50.0%
CCCTCAGCACAACAGTAACA+TGG + tig0030496:6702-6721 None:intergenic 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG + tig0030496:6747-6766 None:intergenic 50.0%
GAGGGAAGTCATTCGAGTGT+AGG - tig0030496:6805-6824 Msa1443660:CDS 50.0%
TTGCTGTGAGAGGAGATTGG+AGG - tig0030496:6844-6863 Msa1443660:CDS 50.0%
CACGTTCGTGCGTTTGGTAA+TGG - tig0030496:6945-6964 Msa1443660:CDS 50.0%
TCTAAGCTGCTGCTGCAAGA+GGG + tig0030496:6006-6025 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG + tig0030496:6007-6026 None:intergenic 50.0%
AGGAGCAAGAGAGAGGAAGA+TGG + tig0030496:6040-6059 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG + tig0030496:6068-6087 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG + tig0030496:6071-6090 None:intergenic 50.0%
GGACCAACAAGGATTTGAGC+AGG + tig0030496:6276-6295 None:intergenic 50.0%
CCCTCAGCACAACAGTAACA+TGG + tig0030496:6702-6721 None:intergenic 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG + tig0030496:6747-6766 None:intergenic 50.0%
GAGGGAAGTCATTCGAGTGT+AGG - tig0030496:6805-6824 Msa1443660:CDS 50.0%
TTGCTGTGAGAGGAGATTGG+AGG - tig0030496:6844-6863 Msa1443660:CDS 50.0%
CACGTTCGTGCGTTTGGTAA+TGG - tig0030496:6945-6964 Msa1443660:CDS 50.0%
TTCCTCTCTCTTGCTCCTCC+TGG - tig0030496:6042-6061 Msa1443660:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG + tig0030496:6063-6082 None:intergenic 55.0%
ATTCCAACGTGTGGGGAGCA+TGG + tig0030496:6971-6990 None:intergenic 55.0%
TTCCTCTCTCTTGCTCCTCC+TGG - tig0030496:6042-6061 Msa1443660:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG + tig0030496:6063-6082 None:intergenic 55.0%
ATTCCAACGTGTGGGGAGCA+TGG + tig0030496:6971-6990 None:intergenic 55.0%
!! GGAGGTATTTGGTGGCCAGG+AGG + tig0030496:6060-6079 None:intergenic 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG + tig0030496:6081-6100 None:intergenic 60.0%
!! GGAGGTATTTGGTGGCCAGG+AGG + tig0030496:6060-6079 None:intergenic 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG + tig0030496:6081-6100 None:intergenic 60.0%
GGCCAGGAGGAGCAAGAGAG+AGG + tig0030496:6047-6066 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG + tig0030496:6078-6097 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG + tig0030496:6082-6101 None:intergenic 65.0%
TGGCCATGCTCCCCACACGT+TGG - tig0030496:6965-6984 Msa1443660:CDS 65.0%
GGCCAGGAGGAGCAAGAGAG+AGG + tig0030496:6047-6066 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG + tig0030496:6078-6097 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG + tig0030496:6082-6101 None:intergenic 65.0%
TGGCCATGCTCCCCACACGT+TGG - tig0030496:6965-6984 Msa1443660:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0030496 gene 5961 7097 5961 ID=Msa1443660;Name=Msa1443660
tig0030496 mRNA 5961 7097 5961 ID=Msa1443660-mRNA-1;Parent=Msa1443660;Name=Msa1443660-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|294
tig0030496 exon 6705 7097 6705 ID=Msa1443660-mRNA-1:exon:14036;Parent=Msa1443660-mRNA-1
tig0030496 exon 5961 6452 5961 ID=Msa1443660-mRNA-1:exon:14035;Parent=Msa1443660-mRNA-1
tig0030496 CDS 6705 7097 6705 ID=Msa1443660-mRNA-1:cds;Parent=Msa1443660-mRNA-1
tig0030496 CDS 5961 6452 5961 ID=Msa1443660-mRNA-1:cds;Parent=Msa1443660-mRNA-1
Gene Sequence

>Msa1443660

ATGCAAACTCATCATCAGTACTACTCATTCTCTTGTGAGAGTCCCTCTTGCAGCAGCAGCTTAGATGATGAAGATTCCATCTTCCTCTCTCTTGCTCCTCCTGGCCACCAAATACCTCCCAAACTCGCACCCCCTCATCCCACTATTACTACTACTATCCCTCCCTTGCATCAAATACCTATTTCTACTACTACCTCTCTTCACCATGACCATACTTGTGCTGTAAATGTAGCACTTAATGCCTTAATTAATCCAACTTTCACTTCCAAACCATCTCACACTCCCATTATTCCTCAATATTGGATCCCTTCTCCTGCTCAAATCCTTGTTGGTCCTACCCAGTTTTCTTGCACTGTCTGCAACAAAACCTTCAACCGTTTCAACAACATGCAGATGCATATGTGGGGACATGGATCTCAATATAGGAAAGGACCGGAGTCTCTAAAAGGGCCAAAACCAGCATCATCTTCATCAATGCTGAAACTACCATGTTACTGTTGTGCTGAGGGTTGCAGAAACAACATAAACTATCCAAGGGCAAGACCACTGAAAGATTTCAGAACCCTTCAAACACACTACAAAAGAAAGCATGGAGGGAAGTCATTCGAGTGTAGGAAGTGTAGGAAGTCATTTGCTGTGAGAGGAGATTGGAGGACACATGAGAAGAATTGTGGGAAGCTTTGGTTTTGTATCTGTGGTTCTGATTTCAAACACAAAAGATCACTAAAAGATCACGTTCGTGCGTTTGGTAATGGCCATGCTCCCCACACGTTGGAATCTTTGAGTTTTGATGGACATAAAGTAAATGAAAATTACATGGAAGAAGAAGAAACTGGAAGTGAAGATGAAGACAATAACAGTGAAGTTGTTTTTAGTTTGAATTAA
Protein sequence

>Msa1443660

MQTHHQYYSFSCESPSCSSSLDDEDSIFLSLAPPGHQIPPKLAPPHPTITTTIPPLHQIPISTTTSLHHDHTCAVNVALNALINPTFTSKPSHTPIIPQYWIPSPAQILVGPTQFSCTVCNKTFNRFNNMQMHMWGHGSQYRKGPESLKGPKPASSSSMLKLPCYCCAEGCRNNINYPRARPLKDFRTLQTHYKRKHGGKSFECRKCRKSFAVRGDWRTHEKNCGKLWFCICGSDFKHKRSLKDHVRAFGNGHAPHTLESLSFDGHKVNENYMEEEETGSEDEDNNSEVVFSLN*