Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1445340 | A0A072TSZ5 | 80.952 | 378 | 70 | 2 | 1 | 376 | 17 | 394 | 0.0 | 576 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1445340 | MtrunA17_Chr8g0377801 | 80.952 | 378 | 70 | 2 | 1 | 376 | 17 | 394 | 0.0 | 576 |
| Msa1445340 | MtrunA17_Chr8g0377781 | 74.531 | 373 | 91 | 2 | 1 | 369 | 1 | 373 | 0.0 | 530 |
| Msa1445340 | MtrunA17_Chr2g0296191 | 27.704 | 379 | 256 | 5 | 1 | 368 | 137 | 508 | 3.21e-37 | 143 |
| Msa1445340 | MtrunA17_Chr2g0296191 | 30.363 | 303 | 206 | 3 | 69 | 367 | 693 | 994 | 5.72e-33 | 130 |
| Msa1445340 | MtrunA17_Chr2g0296711 | 29.431 | 299 | 208 | 2 | 71 | 367 | 86 | 383 | 1.41e-36 | 137 |
| Msa1445340 | MtrunA17_Chr2g0295731 | 26.667 | 300 | 218 | 2 | 69 | 368 | 78 | 375 | 4.12e-35 | 132 |
| Msa1445340 | MtrunA17_Chr4g0067141 | 28.614 | 339 | 233 | 4 | 34 | 369 | 39 | 371 | 4.71e-35 | 132 |
| Msa1445340 | MtrunA17_Chr2g0296721 | 28.247 | 308 | 217 | 3 | 69 | 374 | 81 | 386 | 5.81e-35 | 132 |
| Msa1445340 | MtrunA17_Chr2g0295761 | 27.893 | 337 | 235 | 5 | 34 | 367 | 55 | 386 | 8.41e-35 | 132 |
| Msa1445340 | MtrunA17_Chr2g0286191 | 29.254 | 335 | 228 | 7 | 37 | 368 | 29 | 357 | 1.15e-34 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1445340 | AT5G64950.1 | 38.592 | 355 | 203 | 6 | 24 | 368 | 27 | 376 | 4.29e-73 | 233 |
| Msa1445340 | AT5G07900.1 | 28.406 | 345 | 240 | 5 | 30 | 369 | 59 | 401 | 4.98e-43 | 154 |
| Msa1445340 | AT1G21150.3 | 28.280 | 343 | 235 | 6 | 27 | 364 | 11 | 347 | 5.97e-36 | 134 |
| Msa1445340 | AT1G21150.1 | 29.430 | 316 | 212 | 6 | 54 | 364 | 74 | 383 | 1.92e-35 | 134 |
| Msa1445340 | AT1G21150.2 | 29.430 | 316 | 212 | 6 | 54 | 364 | 138 | 447 | 6.14e-35 | 134 |
| Msa1445340 | AT1G62010.1 | 26.062 | 353 | 217 | 6 | 62 | 374 | 59 | 407 | 1.70e-24 | 104 |
| Msa1445340 | AT1G61990.2 | 24.927 | 341 | 205 | 6 | 73 | 372 | 84 | 414 | 2.02e-24 | 103 |
| Msa1445340 | AT1G61990.1 | 24.927 | 341 | 205 | 6 | 73 | 372 | 84 | 414 | 2.02e-24 | 103 |
| Msa1445340 | AT1G62120.1 | 24.691 | 405 | 249 | 7 | 19 | 374 | 32 | 429 | 7.85e-22 | 96.7 |
| Msa1445340 | AT1G61970.2 | 24.798 | 371 | 232 | 7 | 22 | 353 | 37 | 399 | 8.89e-22 | 96.7 |
Find 63 sgRNAs with CRISPR-Local
Find 168 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGTTCCAAATTTGCTT+AGG | 0.354562 | tig0031043:+16301 | Msa1445340:CDS |
| AAACTAGCAGACAAAATGGT+TGG | 0.477531 | tig0031043:-15895 | Msa1445340:intergenic |
| AAAGGTTCCAAATTTGCTTA+GGG | 0.440166 | tig0031043:+16302 | Msa1445340:CDS |
| AAAGTTTAAAGAAAATGCAG+AGG | 0.668498 | tig0031043:+16557 | Msa1445340:CDS |
| AACAACTTGAGGGATTGCAT+TGG | 0.639420 | tig0031043:-15493 | Msa1445340:intergenic |
| AAGAGAACGTAACAACTTGA+GGG | 0.534047 | tig0031043:-15503 | Msa1445340:intergenic |
| AAGCTATTAAGAAAATTGTA+GGG | 0.601947 | tig0031043:+15944 | Msa1445340:CDS |
| AAGGAGGTTAAACAGAAAAG+TGG | 0.503037 | tig0031043:-15611 | Msa1445340:intergenic |
| AATTCAAGAATGCTTGTTCA+TGG | 0.351341 | tig0031043:+16177 | Msa1445340:CDS |
| ACTCTCTAAGAAAGCAATGT+TGG | 0.518565 | tig0031043:-16037 | Msa1445340:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAACAGAAAAGTGGTTTCT+TGG | - | tig0031043:15606-15625 | None:intergenic | 30.0% | |
| TAGTTTCTAATACGAGATTG+TGG | - | tig0031043:16123-16142 | Msa1445340:intergenic | 30.0% | |
| AATTCAAGAATGCTTGTTCA+TGG | + | tig0031043:16177-16196 | Msa1445340:CDS | 30.0% | |
| ATTCAAGAATGCTTGTTCAT+GGG | + | tig0031043:16178-16197 | Msa1445340:CDS | 30.0% | |
| GTTTGAGCCATAAAACATTT+AGG | + | tig0031043:16217-16236 | Msa1445340:CDS | 30.0% | |
| ATGCTATACATCAAAATCAG+AGG | - | tig0031043:16408-16427 | Msa1445340:intergenic | 30.0% | |
| AAGCTGAAAAACTCTAAATC+GGG | - | tig0031043:16448-16467 | Msa1445340:intergenic | 30.0% | |
| TAAACAGAAAAGTGGTTTCT+TGG | - | tig0031043:15606-15625 | Msa1445340:intergenic | 30.0% | |
| TAGTTTCTAATACGAGATTG+TGG | - | tig0031043:16123-16142 | Msa1445340:intergenic | 30.0% | |
| AATTCAAGAATGCTTGTTCA+TGG | + | tig0031043:16177-16196 | Msa1445340:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0031043 | gene | 15496 | 16632 | 15496 | ID=Msa1445340;Name=Msa1445340 |
| tig0031043 | mRNA | 15496 | 16632 | 15496 | ID=Msa1445340-mRNA-1;Parent=Msa1445340;Name=Msa1445340-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|378 |
| tig0031043 | exon | 15496 | 16632 | 15496 | ID=Msa1445340-mRNA-1:exon:14943;Parent=Msa1445340-mRNA-1 |
| tig0031043 | CDS | 15496 | 16632 | 15496 | ID=Msa1445340-mRNA-1:cds;Parent=Msa1445340-mRNA-1 |
| Gene Sequence |
>Msa1445340 ATGCAATCCCTCAAGTTGTTACGTTCTCTTCCTTTCAGTTCTAACTCACTTCGTTCTTTTCTATGTTCATTTTCTTCTTTATCAACCCCAATTAACCAATCTTCAAACCAAGAAACCACTTTTCTGTTTAACCTCCTTAATTCCAATTCCAGATTACATAAATCTGAATCTATCTATGTATCTAAACTTGTTTCAGGAATCACTTCCCCTGAAAAGCCCTTATCTGTAATCAACTTTTTCAAACAAATTGGATTTTCTCAAACTCAGATACACTCCATCCTTTGTCAGAGAACTCAAATACTCTTTTCAAATGTTCATAAAACCCTTAAACCTAAAGTTGAATTCTTTCAACAATTGGGTTTTCAGGGTTCTCAATTAGGTCTTTTTCTTTCTAAGAATCCAACCATTTTGTCTGCTAGTTTGAACAAAACATTGGTCCCATCTGTGGAAGCTATTAAGAAAATTGTAGGGAATGAGAAAGATCTTATTCAAGTTTTGAGCTACTGTGGCTGGGTACTACCAGTGTACCAAACATTTGTTGCCAACATTGCTTTCTTAGAGAGTTGTGGCATTGTTGGGGATCAGGTTTTGATTTTAATCAAACATTCAAGACTTTTGACTGCACCACAATCTCGTATTAGAAACTATGTTTTGCAAGCTGAAAAATTGGGTTTCCATCAAAATTCAAGAATGCTTGTTCATGGGCTTCATACATTATGTTGTTTGAGCCATAAAACATTTAGGAAAAAGCTTGATTTCATTCAGCGTTTTGGGTTTTCTAAAGATGAGAGCTTGCAAATGTTCAAAAAGGTTCCAAATTTGCTTAGGGTATCAGAGAAGAAGTTAAAGGTTGGAATTGAGTTCTTCTTAGATACAGTTATGCTGCCAAAGTCAGCATTGGTTAATCGGCCTCTGATTTTGATGTATAGCATGGAGGATCGAGTGTTTCCCCGATTTAGAGTTTTTCAGCTTTTGAAATCACAAAATCTATGTAAGAAGGTTCCAAGCTTTCTTTATGTGTTAAATTTATCCGAGGACACATTCTTGGACAAGTTTATACCAAAGTTTAAAGAAAATGCAGAGGCATTATTGATAGCTTACAAAGGGGCGTTGTCTATAACCGCAATGATCTCTTGA |
| Protein sequence |
>Msa1445340 MQSLKLLRSLPFSSNSLRSFLCSFSSLSTPINQSSNQETTFLFNLLNSNSRLHKSESIYVSKLVSGITSPEKPLSVINFFKQIGFSQTQIHSILCQRTQILFSNVHKTLKPKVEFFQQLGFQGSQLGLFLSKNPTILSASLNKTLVPSVEAIKKIVGNEKDLIQVLSYCGWVLPVYQTFVANIAFLESCGIVGDQVLILIKHSRLLTAPQSRIRNYVLQAEKLGFHQNSRMLVHGLHTLCCLSHKTFRKKLDFIQRFGFSKDESLQMFKKVPNLLRVSEKKLKVGIEFFLDTVMLPKSALVNRPLILMYSMEDRVFPRFRVFQLLKSQNLCKKVPSFLYVLNLSEDTFLDKFIPKFKENAEALLIAYKGALSITAMIS* |
