Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1446850 | sp|Q9LFG0|ZAT18_ARATH | 42.138 | 159 | 61 | 5 | 1 | 129 | 1 | 158 | 1.28e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1446850 | Q2HU63 | 94.615 | 130 | 5 | 2 | 1 | 129 | 1 | 129 | 2.39e-81 | 244 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1446850 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1446850 | MtrunA17_Chr2g0327901 | 94.615 | 130 | 5 | 2 | 1 | 129 | 1 | 129 | 4.61e-85 | 244 |
| Msa1446850 | MtrunA17_Chr1g0182181 | 49.333 | 150 | 54 | 5 | 1 | 128 | 1 | 150 | 1.61e-33 | 115 |
| Msa1446850 | MtrunA17_Chr1g0182161 | 48.387 | 155 | 52 | 7 | 1 | 128 | 2 | 155 | 5.00e-33 | 114 |
| Msa1446850 | MtrunA17_Chr7g0263131 | 47.945 | 146 | 50 | 5 | 2 | 126 | 6 | 146 | 1.78e-30 | 107 |
| Msa1446850 | MtrunA17_Chr7g0263121 | 44.295 | 149 | 59 | 6 | 4 | 128 | 9 | 157 | 1.14e-28 | 102 |
| Msa1446850 | MtrunA17_Chr1g0212561 | 37.097 | 186 | 58 | 6 | 1 | 128 | 1 | 185 | 6.72e-26 | 96.7 |
| Msa1446850 | MtrunA17_Chr7g0222831 | 41.916 | 167 | 58 | 5 | 1 | 128 | 1 | 167 | 1.93e-25 | 94.7 |
| Msa1446850 | MtrunA17_Chr7g0231181 | 41.228 | 114 | 54 | 5 | 24 | 126 | 230 | 341 | 1.51e-17 | 77.0 |
| Msa1446850 | MtrunA17_Chr4g0012691 | 47.059 | 85 | 27 | 2 | 34 | 100 | 220 | 304 | 6.87e-17 | 75.1 |
| Msa1446850 | MtrunA17_Chr3g0134711 | 35.156 | 128 | 50 | 3 | 2 | 96 | 37 | 164 | 6.10e-14 | 65.9 |
| Msa1446850 | MtrunA17_Chr1g0207451 | 41.304 | 92 | 45 | 2 | 13 | 96 | 63 | 153 | 8.49e-14 | 65.9 |
| Msa1446850 | MtrunA17_Chr1g0188901 | 33.333 | 138 | 56 | 5 | 18 | 121 | 46 | 181 | 3.42e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1446850 | AT2G28710.1 | 49.324 | 148 | 49 | 4 | 1 | 126 | 1 | 144 | 3.09e-34 | 117 |
| Msa1446850 | AT3G53600.1 | 42.138 | 159 | 61 | 5 | 1 | 129 | 1 | 158 | 1.30e-30 | 108 |
| Msa1446850 | AT5G59820.1 | 56.250 | 96 | 33 | 2 | 7 | 96 | 12 | 104 | 4.01e-29 | 103 |
| Msa1446850 | AT3G46090.1 | 57.732 | 97 | 35 | 2 | 6 | 96 | 12 | 108 | 2.19e-28 | 102 |
| Msa1446850 | AT3G46080.1 | 54.206 | 107 | 25 | 4 | 7 | 98 | 12 | 109 | 2.48e-28 | 102 |
| Msa1446850 | AT2G37430.1 | 40.625 | 160 | 63 | 5 | 1 | 128 | 1 | 160 | 1.22e-25 | 95.5 |
| Msa1446850 | AT3G46070.1 | 50.000 | 102 | 24 | 3 | 12 | 96 | 17 | 108 | 2.98e-23 | 89.4 |
| Msa1446850 | AT2G45120.1 | 51.316 | 76 | 28 | 1 | 34 | 100 | 194 | 269 | 4.77e-18 | 78.2 |
| Msa1446850 | AT5G03510.1 | 35.616 | 146 | 39 | 4 | 6 | 96 | 69 | 214 | 1.35e-17 | 77.0 |
| Msa1446850 | AT5G43170.1 | 44.565 | 92 | 39 | 3 | 13 | 96 | 53 | 140 | 6.50e-17 | 73.6 |
| Msa1446850 | AT3G60580.1 | 40.187 | 107 | 49 | 4 | 34 | 125 | 173 | 279 | 7.11e-16 | 72.0 |
| Msa1446850 | AT1G02030.1 | 41.237 | 97 | 45 | 2 | 34 | 121 | 160 | 253 | 4.16e-15 | 69.7 |
| Msa1446850 | AT1G49900.1 | 38.679 | 106 | 55 | 3 | 30 | 127 | 746 | 849 | 8.59e-14 | 67.0 |
| Msa1446850 | AT1G49900.1 | 42.667 | 75 | 31 | 1 | 34 | 96 | 193 | 267 | 7.98e-12 | 61.6 |
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAGGGCATGGATGCATCTT+TGG | 0.291358 | tig0031500:+30397 | None:intergenic |
| CCACATTCTTTCAATGTATT+AGG | 0.293992 | tig0031500:+30532 | None:intergenic |
| GTCTCTTGTGACTTGCTCTA+TGG | 0.295656 | tig0031500:+30455 | None:intergenic |
| GAAAACTCAAGTCCACAAAT+AGG | 0.295855 | tig0031500:+30379 | None:intergenic |
| TGCATCTTTGGTTTCATGTT+TGG | 0.322903 | tig0031500:+30409 | None:intergenic |
| TACTATTATGCTTTCTCATA+TGG | 0.326158 | tig0031500:+30335 | None:intergenic |
| AACTTGTACATGAGTACTTT+CGG | 0.412472 | tig0031500:+30433 | None:intergenic |
| ATGCATCCATGCCCTATTTG+TGG | 0.444120 | tig0031500:-30391 | Msa1446850:CDS |
| AAAACTCAAGTCCACAAATA+GGG | 0.449315 | tig0031500:+30380 | None:intergenic |
| TCCATTGGACAAGCTCTTGG+TGG | 0.462440 | tig0031500:-30358 | Msa1446850:CDS |
| AACTTGACGCCGTTAGAGAA+TGG | 0.480904 | tig0031500:-30250 | Msa1446850:CDS |
| ACTTGCTCTATGGCCACCTA+AGG | 0.522596 | tig0031500:+30465 | None:intergenic |
| CCTAATACATTGAAAGAATG+TGG | 0.550971 | tig0031500:-30532 | Msa1446850:CDS |
| TTGATGCTACTATCCAAAGT+TGG | 0.579792 | tig0031500:-30568 | Msa1446850:CDS |
| CAAGCAATGCCATTCTCTAA+CGG | 0.581534 | tig0031500:+30241 | None:intergenic |
| TCAAGTCCACAAATAGGGCA+TGG | 0.590188 | tig0031500:+30385 | None:intergenic |
| GCCACCAAGAGCTTGTCCAA+TGG | 0.605063 | tig0031500:+30357 | None:intergenic |
| TAAGTGCAAAACATGCAACA+AGG | 0.649557 | tig0031500:-30506 | Msa1446850:CDS |
| TTGTGCGTAGACATGAAGAG+AGG | 0.664491 | tig0031500:-30619 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TACTATTATGCTTTCTCATA+TGG | + | tig0031500:30515-30534 | None:intergenic | 25.0% |
| !!! | ATCTTTTGATTTCTTGAAGA+TGG | + | tig0031500:30544-30563 | None:intergenic | 25.0% |
| CCACATTCTTTCAATGTATT+AGG | + | tig0031500:30318-30337 | None:intergenic | 30.0% | |
| CCTAATACATTGAAAGAATG+TGG | - | tig0031500:30315-30334 | Msa1446850:CDS | 30.0% | |
| AACTTGTACATGAGTACTTT+CGG | + | tig0031500:30417-30436 | None:intergenic | 30.0% | |
| AAAACTCAAGTCCACAAATA+GGG | + | tig0031500:30470-30489 | None:intergenic | 30.0% | |
| TTGATGCTACTATCCAAAGT+TGG | - | tig0031500:30279-30298 | Msa1446850:CDS | 35.0% | |
| !! | CTCTTTTGTTTCACCAACTT+TGG | + | tig0031500:30295-30314 | None:intergenic | 35.0% |
| TAAGTGCAAAACATGCAACA+AGG | - | tig0031500:30341-30360 | Msa1446850:CDS | 35.0% | |
| !! | TTCTCTTCTTTTCAAGCCTT+AGG | - | tig0031500:30366-30385 | Msa1446850:CDS | 35.0% |
| ! | TGCATCTTTGGTTTCATGTT+TGG | + | tig0031500:30441-30460 | None:intergenic | 35.0% |
| GAAAACTCAAGTCCACAAAT+AGG | + | tig0031500:30471-30490 | None:intergenic | 35.0% | |
| !! | TCTTCTTTTCAAGCCTTAGG+TGG | - | tig0031500:30369-30388 | Msa1446850:CDS | 40.0% |
| ! | TGTGGACTTGAGTTTTCCAT+TGG | - | tig0031500:30474-30493 | Msa1446850:CDS | 40.0% |
| ! | TTTTCCATTGGACAAGCTCT+TGG | - | tig0031500:30486-30505 | Msa1446850:CDS | 40.0% |
| CAAGCAATGCCATTCTCTAA+CGG | + | tig0031500:30609-30628 | None:intergenic | 40.0% | |
| !! | GTCTCTTGTGACTTGCTCTA+TGG | + | tig0031500:30395-30414 | None:intergenic | 45.0% |
| ATAGGGCATGGATGCATCTT+TGG | + | tig0031500:30453-30472 | None:intergenic | 45.0% | |
| ATGCATCCATGCCCTATTTG+TGG | - | tig0031500:30456-30475 | Msa1446850:CDS | 45.0% | |
| TCAAGTCCACAAATAGGGCA+TGG | + | tig0031500:30465-30484 | None:intergenic | 45.0% | |
| AACTTGACGCCGTTAGAGAA+TGG | - | tig0031500:30597-30616 | Msa1446850:CDS | 45.0% | |
| ACTTGCTCTATGGCCACCTA+AGG | + | tig0031500:30385-30404 | None:intergenic | 50.0% | |
| TCCATTGGACAAGCTCTTGG+TGG | - | tig0031500:30489-30508 | Msa1446850:CDS | 50.0% | |
| GCCACCAAGAGCTTGTCCAA+TGG | + | tig0031500:30493-30512 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0031500 | gene | 30240 | 30629 | 30240 | ID=Msa1446850;Name=Msa1446850 |
| tig0031500 | mRNA | 30240 | 30629 | 30240 | ID=Msa1446850-mRNA-1;Parent=Msa1446850;Name=Msa1446850-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|129 |
| tig0031500 | exon | 30240 | 30629 | 30240 | ID=Msa1446850-mRNA-1:exon:15602;Parent=Msa1446850-mRNA-1 |
| tig0031500 | CDS | 30240 | 30629 | 30240 | ID=Msa1446850-mRNA-1:cds;Parent=Msa1446850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
