AlfalfaGEDB | Msa1447810

M. sativa cultivar zhongmu-4 / Msa1447810

KEGG: K03083
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447810	sp\|P51139\|MSK3_MEDSA	87.013	77	5	1	15	91	237	308	1.12e-37	137

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447810	A0A392NZV8	87.013	77	5	1	15	91	12	83	1.65e-37	137

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447810	MtrunA17_Chr7g0270371	87.013	77	5	1	15	91	238	309	6.60e-39	138
Msa1447810	MtrunA17_Chr1g0193081	85.714	77	6	1	15	91	238	309	7.20e-38	135
Msa1447810	MtrunA17_Chr1g0194201	84.416	77	7	1	15	91	238	309	8.22e-38	135
Msa1447810	MtrunA17_Chr2g0277011	84.416	77	7	1	15	91	253	324	3.70e-37	134
Msa1447810	MtrunA17_Chr7g0246591	81.818	77	9	1	15	91	304	375	7.82e-37	133
Msa1447810	MtrunA17_Chr8g0350311	80.519	77	10	1	15	91	302	373	1.00e-35	130
Msa1447810	MtrunA17_Chr2g0320011	80.519	77	10	1	15	91	251	322	2.25e-35	129
Msa1447810	MtrunA17_Chr2g0284091	75.325	77	14	1	15	91	203	274	4.62e-34	124
Msa1447810	MtrunA17_Chr4g0030241	38.750	80	44	1	14	93	63	137	6.82e-14	67.0
Msa1447810	MtrunA17_Chr7g0273601	40.000	95	46	3	6	94	261	350	1.51e-13	68.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1447810	AT5G14640.1	87.013	77	5	1	15	91	237	308	1.89e-38	137
Msa1447810	AT5G14640.2	87.013	77	5	1	15	91	237	308	1.89e-38	137
Msa1447810	AT1G09840.11	83.117	77	8	1	15	91	119	190	9.68e-38	132
Msa1447810	AT3G05840.2	84.416	77	7	1	15	91	236	307	1.64e-37	134
Msa1447810	AT3G05840.1	84.416	77	7	1	15	91	236	307	1.64e-37	134
Msa1447810	AT5G26751.1	84.416	77	7	1	15	91	232	303	1.97e-37	134
Msa1447810	AT1G57870.1	84.416	77	7	1	15	91	245	316	4.29e-37	134
Msa1447810	AT1G57870.4	84.416	77	7	1	15	91	245	316	4.29e-37	134
Msa1447810	AT1G57870.2	84.416	77	7	1	15	91	245	316	4.29e-37	134
Msa1447810	AT1G09840.3	83.117	77	8	1	15	91	246	317	7.11e-37	133

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Find 43 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAATGTAAGATATGTT+TGG	0.394876	tig0031943:+36694	Msa1447810:intergenic
AATTTAAACTCTGTATAATT+AGG	0.220898	tig0031943:+36232	Msa1447810:intergenic
ACAAACCCTTATCACCCTAT+AGG	0.444255	tig0031943:+34989	Msa1447810:intergenic
ACCAACTGAGCAGATGTCAA+TGG	0.572722	tig0031943:+36617	Msa1447810:intergenic
ACCATTGACATCTGCTCAGT+TGG	0.489208	tig0031943:-36618	Msa1447810:CDS
ACTTGATTTCTTCCCTTGTT+GGG	0.311805	tig0031943:+36263	Msa1447810:intergenic
AGAGCTCCTGAGCTTATATT+CGG	0.474562	tig0031943:-36660	Msa1447810:CDS
AGATAAACAATGTGCTCATT+TGG	0.319034	tig0031943:-35217	Msa1447810:CDS
AGGAACCATCATACAAAGCA+TGG	0.519567	tig0031943:+35009	Msa1447810:intergenic
AGTCGCATATCTTCAGTTGG+TGG	0.661802	tig0031943:+36838	Msa1447800:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAACGAAAGGATTTCA+AGG	+	tig0031943:34844-34863	Msa1447810:intergenic	30.0%
CAAACTTGTGGAAATTATCA+AGG	-	tig0031943:35171-35190	Msa1447810:intron	30.0%
ATATGTTCAAGATAGTAGCA+AGG	+	tig0031943:35239-35258	Msa1447810:intergenic	30.0%
CAAATCTCATTCATGACATA+AGG	-	tig0031943:35421-35440	Msa1447810:intron	30.0%
TTCATTCAAACTGAATGTGA+AGG	+	tig0031943:35668-35687	Msa1447810:intergenic	30.0%
CAAATCAACTTTATGTCAGT+TGG	+	tig0031943:35709-35728	Msa1447810:intergenic	30.0%
TTAAGCTCTTAAGTCTTCAT+CGG	+	tig0031943:35931-35950	Msa1447810:intergenic	30.0%
ACTTAAGAGCTTAAGGATAA+AGG	-	tig0031943:35937-35956	Msa1447810:intron	30.0%
TTCAATCAAATTTGGCATGT+AGG	+	tig0031943:36048-36067	Msa1447810:intergenic	30.0%
AAAATGAAAATAGGGAACCA+TGG	-	tig0031943:36125-36144	Msa1447810:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0031943	gene	34752	36891	34752	ID=Msa1447810;Name=Msa1447810
tig0031943	mRNA	34752	36891	34752	ID=Msa1447810-mRNA-1;Parent=Msa1447810;Name=Msa1447810-mRNA-1;_AED=0.58;_eAED=0.58;_QI=66\|0.16\|0.14\|0.57\|0.83\|0.85\|7\|0\|209
tig0031943	exon	34752	34844	34752	ID=Msa1447810-mRNA-1:exon:16101;Parent=Msa1447810-mRNA-1
tig0031943	exon	34929	35072	34929	ID=Msa1447810-mRNA-1:exon:16100;Parent=Msa1447810-mRNA-1
tig0031943	exon	35203	35323	35203	ID=Msa1447810-mRNA-1:exon:16099;Parent=Msa1447810-mRNA-1
tig0031943	exon	36241	36296	36241	ID=Msa1447810-mRNA-1:exon:16098;Parent=Msa1447810-mRNA-1
tig0031943	exon	36451	36501	36451	ID=Msa1447810-mRNA-1:exon:16097;Parent=Msa1447810-mRNA-1
tig0031943	exon	36572	36728	36572	ID=Msa1447810-mRNA-1:exon:16096;Parent=Msa1447810-mRNA-1
tig0031943	exon	36818	36891	36818	ID=Msa1447810-mRNA-1:exon:16095;Parent=Msa1447810-mRNA-1
tig0031943	five_prime_UTR	36826	36891	36826	ID=Msa1447810-mRNA-1:five_prime_utr;Parent=Msa1447810-mRNA-1
tig0031943	CDS	36818	36825	36818	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1
tig0031943	CDS	36572	36728	36572	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1
tig0031943	CDS	36451	36501	36451	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1
tig0031943	CDS	36241	36296	36241	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1
tig0031943	CDS	35203	35323	35203	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1
tig0031943	CDS	34929	35072	34929	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1
tig0031943	CDS	34752	34844	34752	ID=Msa1447810-mRNA-1:cds;Parent=Msa1447810-mRNA-1

Gene Sequence

>Msa1447810

ATGTCTTGGTTAAAGGTGAACCAAACATATCTTACATTTGTTTCTAGGTACTATAGAGCTCCTGAGCTTATATTCGGTGCAACTGAGTACACCACAACCATTGACATCTGCTCAGTTGGTTGCGTACTTGGTGAACTTTTGCTTGGCCAGTTAACTAGCTTCTGTCCACTGTTTCCTAGTGAGAGTGGAGTAGACAAACTTGTGGAAATTATCAAGGTTTTAGGTACCCCAACAAGGGAAGAAATCAAGTGCATGAATCCTAATTATACAGAACCGTTTAAGGTTAAACTCCAAGCATCTGATGTAACTGATGATGCAGTGTTTGTTGCTTTTGACACTGACATGGGTTTTCTTCTAGATAAACAATGTGCTCATTTGGTTGATGTAGCTAAGGGGAAGAAGATGCTTTTTAAAGTTCAGGCAGTTATTGGACCCCATGCTTTGTATGATGGTTCCTATAGGGTGATAAGGGTTTGTATTGATGAAGCTATTATTGAATTGTTTCTCACTGATGATGAGATTTACTCTCCTTCTAAGGCGTTGACTGTTTCACTTAATGATGAGGTTATTGATACTGAGAAGATTGTTGGCTCGGTTGATGCTTCGCAGACTGAGGATTTTGTCTCTTAA

Protein sequence

>Msa1447810

MSWLKVNQTYLTFVSRYYRAPELIFGATEYTTTIDICSVGCVLGELLLGQLTSFCPLFPSESGVDKLVEIIKVLGTPTREEIKCMNPNYTEPFKVKLQASDVTDDAVFVAFDTDMGFLLDKQCAHLVDVAKGKKMLFKVQAVIGPHALYDGSYRVIRVCIDEAIIELFLTDDEIYSPSKALTVSLNDEVIDTEKIVGSVDASQTEDFVS*