AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448550 MtrunA17_Chr2g0296781 72.872 564 110 1 97 617 1 564 0.0 863
Msa1448550 MtrunA17_Chr1g0196541 63.943 563 160 1 97 616 1 563 0.0 746
Msa1448550 MtrunA17_Chr6g0477261 76.770 452 62 1 209 617 2 453 0.0 720
Msa1448550 MtrunA17_Chr5g0441581 45.890 584 259 4 76 616 21 590 1.28e-176 517
Msa1448550 MtrunA17_Chr1g0207551 34.347 559 328 4 92 611 33 591 4.25e-111 352
Msa1448550 MtrunA17_Chr2g0291581 35.106 564 308 7 93 610 71 622 3.86e-110 346
Msa1448550 MtrunA17_Chr2g0291591 33.643 538 333 8 38 566 15 537 1.11e-106 333
Msa1448550 MtrunA17_Chr5g0448761 35.428 573 318 13 88 609 54 625 2.84e-106 338
Msa1448550 MtrunA17_Chr2g0329031 35.081 496 300 6 82 567 1 484 5.87e-106 336
Msa1448550 MtrunA17_Chr1g0202341 34.507 568 316 11 92 610 50 610 2.88e-105 333
Msa1448550 MtrunA17_Chr3g0080351 33.507 576 306 9 92 613 75 627 1.09e-98 320
Msa1448550 MtrunA17_Chr5g0440451 31.220 631 338 17 58 617 7 612 4.52e-83 280
Msa1448550 MtrunA17_Chr8g0363841 31.992 497 308 8 91 572 12 493 4.16e-75 253
Msa1448550 MtrunA17_Chr3g0118611 40.062 322 190 2 262 582 118 437 2.47e-74 253
Msa1448550 MtrunA17_Chr5g0448511 37.975 316 194 2 250 564 1 315 2.59e-74 248
Msa1448550 MtrunA17_Chr2g0324011 29.691 485 313 10 92 559 9 482 2.98e-70 238
Msa1448550 MtrunA17_Chr2g0287651 27.850 535 354 9 85 610 67 578 2.09e-67 236
Msa1448550 MtrunA17_Chr2g0287061 31.818 484 291 9 87 570 177 621 2.27e-65 229
Msa1448550 MtrunA17_Chr2g0276841 28.246 570 340 20 93 606 70 626 6.04e-62 218
Msa1448550 MtrunA17_Chr3g0082121 32.371 485 290 16 62 527 10 475 9.74e-62 217
Msa1448550 MtrunA17_Chr1g0203051 29.150 494 329 10 50 533 38 520 1.38e-61 216
Msa1448550 MtrunA17_Chr1g0198761 30.636 519 324 14 96 590 50 556 3.37e-61 217
Msa1448550 MtrunA17_Chr1g0203721 27.918 437 307 5 95 523 65 501 7.12e-57 206
Msa1448550 MtrunA17_Chr6g0458391 25.606 578 366 11 77 609 207 765 3.72e-56 202
Msa1448550 MtrunA17_Chr5g0431001 30.233 430 268 11 93 498 61 482 7.33e-56 197
Msa1448550 MtrunA17_Chr2g0294841 27.664 488 312 11 63 529 15 482 9.39e-55 199
Msa1448550 MtrunA17_Chr1g0164591 32.075 318 198 8 170 480 2 308 5.64e-47 168
Msa1448550 MtrunA17_Chr7g0258861 32.028 281 184 4 92 365 9 289 1.29e-43 158
Msa1448550 MtrunA17_Chr2g0290391 23.698 557 369 16 71 591 22 558 1.63e-40 157
Msa1448550 MtrunA17_Chr6g0476431 55.814 86 38 0 97 182 1 86 1.68e-27 106
Msa1448550 MtrunA17_Chr6g0451441 31.414 191 119 6 70 254 63 247 5.81e-17 84.0
Msa1448550 MtrunA17_Chr7g0233911 35.246 122 69 6 91 205 32 150 4.58e-12 65.9
Msa1448550 MtrunA17_Chr2g0308841 27.536 138 94 2 421 557 6 138 6.23e-12 66.6
Msa1448550 MtrunA17_Chr1g0149131 28.182 110 78 1 367 475 1 110 2.29e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448550 AT4G15090.1 34.418 584 332 8 71 609 32 609 6.79e-111 352
Msa1448550 AT4G15090.5 34.418 584 332 8 71 609 32 609 6.79e-111 352
Msa1448550 AT4G15090.3 34.418 584 332 8 71 609 32 609 6.79e-111 352
Msa1448550 AT4G15090.2 34.418 584 332 8 71 609 32 609 6.79e-111 352
Msa1448550 AT4G15090.4 34.418 584 332 8 71 609 32 609 6.79e-111 352
Msa1448550 AT4G38180.1 36.100 482 298 6 92 564 72 552 5.26e-103 330
Msa1448550 AT3G22170.2 32.696 575 311 7 92 612 69 621 6.95e-102 328
Msa1448550 AT3G22170.1 32.696 575 311 7 92 612 69 621 6.95e-102 328
Msa1448550 AT1G76320.3 35.331 484 300 5 97 575 1 476 9.95e-99 316
Msa1448550 AT1G76320.2 32.496 557 329 7 97 614 1 549 3.38e-98 316
Msa1448550 AT1G76320.1 32.496 557 329 7 97 614 1 549 4.56e-98 316
Msa1448550 AT1G76320.4 32.496 557 329 7 97 614 1 549 4.56e-98 316
Msa1448550 AT1G52520.1 31.707 574 321 13 93 612 85 641 4.90e-95 307
Msa1448550 AT1G80010.1 31.293 588 343 13 79 610 80 662 3.09e-92 300
Msa1448550 AT2G27110.2 35.729 473 273 11 92 560 48 493 1.57e-82 277
Msa1448550 AT2G27110.1 35.729 473 273 11 92 560 48 493 1.57e-82 277
Msa1448550 AT4G19990.5 30.450 578 306 13 96 614 24 564 1.11e-77 262
Msa1448550 AT4G19990.3 30.450 578 306 13 96 614 24 564 1.26e-77 262
Msa1448550 AT4G19990.4 30.450 578 306 13 96 614 24 564 1.26e-77 262
Msa1448550 AT4G19990.7 30.450 578 306 13 96 614 38 578 1.49e-77 262
Msa1448550 AT4G19990.6 30.450 578 306 13 96 614 24 564 1.98e-77 262
Msa1448550 AT4G19990.2 30.450 578 306 13 96 614 24 564 1.98e-77 262
Msa1448550 AT2G32250.5 30.137 584 321 11 70 606 7 550 7.02e-77 260
Msa1448550 AT2G32250.2 30.137 584 321 11 70 606 7 550 1.11e-76 260
Msa1448550 AT2G32250.4 30.137 584 321 11 70 606 7 550 1.11e-76 260
Msa1448550 AT2G32250.6 30.137 584 321 11 70 606 7 550 1.12e-76 260
Msa1448550 AT2G32250.1 30.137 584 321 11 70 606 7 550 1.12e-76 260
Msa1448550 AT2G32250.3 30.137 584 321 11 70 606 7 550 1.12e-76 260
Msa1448550 AT2G32250.7 30.601 549 302 9 97 606 1 509 4.56e-75 255
Msa1448550 AT2G32250.8 30.601 549 302 9 97 606 1 509 5.04e-75 255
Msa1448550 AT2G27110.3 38.439 346 200 5 217 560 14 348 2.45e-74 252
Msa1448550 AT4G19990.1 30.147 544 288 13 115 614 12 507 5.82e-73 248
Msa1448550 AT5G18960.2 30.550 491 297 11 75 560 191 642 1.39e-68 238
Msa1448550 AT5G18960.1 30.550 491 297 11 75 560 191 642 1.39e-68 238
Msa1448550 AT3G06250.2 29.485 485 297 10 80 561 177 619 4.11e-67 233
Msa1448550 AT3G06250.1 29.485 485 297 10 80 561 177 619 4.11e-67 233
Msa1448550 AT3G06250.3 29.485 485 297 10 80 561 177 619 4.11e-67 233
Msa1448550 AT4G38170.1 31.899 395 226 5 258 613 13 403 5.75e-65 223
Msa1448550 AT1G10240.2 26.010 619 382 21 43 606 7 604 8.86e-57 204
Msa1448550 AT1G10240.1 26.010 619 382 21 43 606 7 604 8.86e-57 204
Msa1448550 AT5G28530.3 24.340 530 348 16 93 591 55 562 1.88e-40 157
Msa1448550 AT5G28530.2 24.340 530 348 16 93 591 55 562 1.88e-40 157
Msa1448550 AT5G28530.1 24.345 534 347 17 93 591 55 566 1.47e-39 154
Msa1448550 AT5G28530.5 23.608 521 300 19 93 591 55 499 1.49e-24 108
Msa1448550 AT5G28530.4 23.619 525 299 20 93 591 55 503 9.54e-24 106

Find 91 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATAGCTCTTGTAATATT+TGG 0.170681 tig0032222:-8087 None:intergenic
TGTATGTTAGAGACACATTT+TGG 0.182163 tig0032222:+9176 Msa1448550:CDS
TCTATCTTGGTCAGTAATTA+TGG 0.209922 tig0032222:-8891 None:intergenic
GTCGTTTGCGAAATGTATTC+TGG 0.220344 tig0032222:+8639 Msa1448550:CDS
TTCGAGCATATCTGATTTCA+TGG 0.264868 tig0032222:+9063 Msa1448550:CDS
CCTTCTTCATTGCTCTATCT+TGG 0.275947 tig0032222:-8904 None:intergenic
CCGTATCCTCATTCGACAAT+AGG 0.297761 tig0032222:-8808 None:intergenic
CACCATCGATGGCGAGCTTT+CGG 0.297833 tig0032222:-8944 None:intergenic
TTTGTGGCATTTAATGAAAA+AGG 0.306478 tig0032222:+8967 Msa1448550:CDS
TTGTGTTATAGAAAGTAAAA+AGG 0.307430 tig0032222:+9510 Msa1448550:CDS
AAGCAGAAATTTCCGATCTA+TGG 0.310416 tig0032222:+8436 Msa1448550:CDS
AGGGCTGCTTATGAGTATTT+TGG 0.315150 tig0032222:+8677 Msa1448550:CDS
TATGCTAGATGTATGGGTTT+TGG 0.332468 tig0032222:+8104 Msa1448550:CDS
AGCCATACACTGTGCTCTTA+TGG 0.342605 tig0032222:-8243 None:intergenic
GGATATGATAATCTTACATT+TGG 0.343496 tig0032222:+8473 Msa1448550:CDS
TGTGCTCTTATGGTAGGATT+TGG 0.353524 tig0032222:-8233 None:intergenic
CGTATCCTCATTCGACAATA+GGG 0.366937 tig0032222:-8807 None:intergenic
AATAGGTGTATGTCTTTAGA+TGG 0.367624 tig0032222:+8272 Msa1448550:CDS
ATTTAGACCCTCATACAAAA+AGG 0.380265 tig0032222:+8378 Msa1448550:CDS
GTGGAATTGAATGTTGAAAC+AGG 0.383950 tig0032222:-7796 None:intergenic
GTACTGTAATGCTTTCTTAG+AGG 0.385678 tig0032222:+9462 Msa1448550:CDS
CAAGAGCTATGCTAGATGTA+TGG 0.393295 tig0032222:+8097 Msa1448550:CDS
TGGTCAGTAATTATGGCATT+TGG 0.404502 tig0032222:-8884 None:intergenic
ATATTTATTTGTTAAATAAG+TGG 0.416959 tig0032222:-8720 None:intergenic
AAGCAAGACGGCTTCGACTT+GGG 0.424631 tig0032222:+8525 Msa1448550:CDS
TTATGTGATCAACCTCCTCT+TGG 0.424715 tig0032222:-7971 None:intergenic
AGTATGAATTCATTCTTTGA+TGG 0.436716 tig0032222:+9229 Msa1448550:CDS
GTTGCTCTTGAGCATAATCA+TGG 0.447972 tig0032222:+8314 Msa1448550:CDS
TGGTAGGATTTGGTTTCAAA+AGG 0.448606 tig0032222:-8223 None:intergenic
AGTGTATGGCTAGAGTAAAT+AGG 0.451633 tig0032222:+8255 Msa1448550:CDS
CTCGCCATCGATGGTGTTTG+TGG 0.452930 tig0032222:+8951 Msa1448550:CDS
TGATGATTGACTCCAACAAA+AGG 0.455391 tig0032222:-8758 None:intergenic
TAATGCTTTCTTAGAGGGAA+TGG 0.460956 tig0032222:+9468 Msa1448550:CDS
GAAGCAAATATTGTTCAAGC+AGG 0.462377 tig0032222:+8017 Msa1448550:CDS
GTATGAATTCATTCTTTGAT+GGG 0.486422 tig0032222:+9230 Msa1448550:CDS
CTAAATGTCATCCCAATCCT+AGG 0.488080 tig0032222:-8047 None:intergenic
CTTTGTGTTCTTCAGCTCAC+AGG 0.489432 tig0032222:+9646 Msa1448550:intron
ACTCCAACAAAAGGGGCAAA+AGG 0.489968 tig0032222:-8749 None:intergenic
TACCATAAGAGCACAGTGTA+TGG 0.490683 tig0032222:+8241 Msa1448550:CDS
GCAGGTGAGGAACCTAGGAT+TGG 0.494746 tig0032222:+8035 Msa1448550:CDS
TCTGGGTAGATGCGAGATGT+AGG 0.494755 tig0032222:+8657 Msa1448550:CDS
TACTGTAATGCTTTCTTAGA+GGG 0.499926 tig0032222:+9463 Msa1448550:CDS
ATTTGATGAGAGAGATCGTT+GGG 0.500194 tig0032222:+9147 Msa1448550:CDS
TCGTTTGCGAAATGTATTCT+GGG 0.501285 tig0032222:+8640 Msa1448550:CDS
TCTATGGTTGTTGAAGCAAA+TGG 0.501556 tig0032222:+8452 Msa1448550:CDS
TTCCGAAAGCTCGCCATCGA+TGG 0.503126 tig0032222:+8942 Msa1448550:CDS
AAAGCAAGACGGCTTCGACT+TGG 0.506465 tig0032222:+8524 Msa1448550:CDS
AAGAGCTATGCTAGATGTAT+GGG 0.507368 tig0032222:+8098 Msa1448550:CDS
CCTATTGTCGAATGAGGATA+CGG 0.509576 tig0032222:+8808 Msa1448550:CDS
GTTGAAAGATAAAATTGAAA+AGG 0.510250 tig0032222:+9306 Msa1448550:CDS
GATGATTGACTCCAACAAAA+GGG 0.511990 tig0032222:-8757 None:intergenic
GGTTAGAATGCATGCATGGA+CGG 0.519306 tig0032222:+8858 Msa1448550:CDS
CAGGTGAGGAACCTAGGATT+GGG 0.519505 tig0032222:+8036 Msa1448550:CDS
CTGGGTAGATGCGAGATGTA+GGG 0.525010 tig0032222:+8658 Msa1448550:CDS
CATGATAATGAATGGTTGAA+AGG 0.528750 tig0032222:+9121 Msa1448550:CDS
AGCAATGAAGAAGGCAATTG+AGG 0.534217 tig0032222:+8913 Msa1448550:CDS
GTTAGAATGCATGCATGGAC+GGG 0.537622 tig0032222:+8859 Msa1448550:CDS
AGACGGCTTCGACTTGGGAT+AGG 0.543565 tig0032222:+8530 Msa1448550:CDS
AGTAAAGTGATGTTTGAGGA+TGG 0.543909 tig0032222:-7765 None:intergenic
ATGATAATGAATGGTTGAAA+GGG 0.544915 tig0032222:+9122 Msa1448550:CDS
TCAAAGTAAAGTGATGTTTG+AGG 0.547191 tig0032222:-7769 None:intergenic
CCAAGATAGAGCAATGAAGA+AGG 0.549366 tig0032222:+8904 Msa1448550:CDS
TAAAATTGAAAAGGAAAGCA+TGG 0.555126 tig0032222:+9315 Msa1448550:CDS
TTGCTTCAACAACCATAGAT+CGG 0.559940 tig0032222:-8448 None:intergenic
TTCAAGCAGGTGAGGAACCT+AGG 0.562056 tig0032222:+8030 Msa1448550:CDS
CGCTATTGTATTCACCGCCA+CGG 0.563090 tig0032222:+7701 Msa1448550:CDS
GGTGAATACAATAGCGGCGA+TGG 0.581161 tig0032222:-7694 None:intergenic
TTTCTTGGTTGTTCAAAACA+TGG 0.584090 tig0032222:+8837 Msa1448550:CDS
TATCTGATTTCATGGAGAAG+TGG 0.590848 tig0032222:+9071 Msa1448550:CDS
ATACACTGTGCTCTTATGGT+AGG 0.596198 tig0032222:-8239 None:intergenic
TTGTGCCCTATTGTCGAATG+AGG 0.600198 tig0032222:+8802 Msa1448550:CDS
AAATATTGTTCAAGCAGGTG+AGG 0.602230 tig0032222:+8022 Msa1448550:CDS
TTAGATATTAATGATCAAGC+TGG 0.603736 tig0032222:+8404 Msa1448550:CDS
CAACAGCGACGAGACGACAA+AGG 0.606712 tig0032222:-7659 None:intergenic
ATGAACTGCATGATAATGAA+TGG 0.623376 tig0032222:+9113 Msa1448550:CDS
TTTGTTGGAGTCAATCATCA+CGG 0.624437 tig0032222:+8761 Msa1448550:CDS
ATGATTGACTCCAACAAAAG+GGG 0.626051 tig0032222:-8756 None:intergenic
ACATGGTTAGAATGCATGCA+TGG 0.634260 tig0032222:+8854 Msa1448550:CDS
AATGCCACAAACACCATCGA+TGG 0.638359 tig0032222:-8955 None:intergenic
AATGTGTCTCTAACATACAC+AGG 0.644327 tig0032222:-9172 None:intergenic
TAGTTGTGCGGCGAGAACCG+TGG 0.647045 tig0032222:-7718 None:intergenic
TATTTGATGAGAGAGATCGT+TGG 0.649110 tig0032222:+9146 Msa1448550:CDS
GGAATTGAATGTTGAAACAG+GGG 0.654531 tig0032222:-7794 None:intergenic
TGGAATTGAATGTTGAAACA+GGG 0.655246 tig0032222:-7795 None:intergenic
ATTACATTGACAAAGCAAGA+CGG 0.657095 tig0032222:+8513 Msa1448550:CDS
CTTGACAAAATATAACAAGA+CGG 0.676618 tig0032222:-9685 None:intergenic
GGTGGAGGAAGGATATTAAG+CGG 0.683033 tig0032222:+9707 Msa1448550:CDS
GATATGCAGTTATAGTTGTG+CGG 0.693838 tig0032222:-7730 None:intergenic
ACATATAAAGATGAATCATG+CGG 0.695682 tig0032222:+7232 None:intergenic
TTGTGCGGCGAGAACCGTGG+CGG 0.697644 tig0032222:-7715 None:intergenic
CGTGGCGGTGAATACAATAG+CGG 0.730647 tig0032222:-7700 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTTTTTTAATCAAAATTT+AGG - tig0032222:7351-7370 None:intergenic 10.0%
!!! ATATTTATTTGTTAAATAAG+TGG - tig0032222:8723-8742 None:intergenic 10.0%
!! TTCAACTTTACTAAAAAAAA+AGG - tig0032222:7327-7346 None:intergenic 15.0%
!! CAAAATCATTAAGAATAAAA+AGG - tig0032222:7540-7559 None:intergenic 15.0%
!!! TAAAATATCTAGATTTTGTT+GGG - tig0032222:8346-8365 None:intergenic 15.0%
!! AAATTATTCTTACTTACTTG+TGG - tig0032222:7818-7837 None:intergenic 20.0%
!! ACAAGTAAGTAAGAATAATT+TGG + tig0032222:7817-7836 Msa1448550:intron 20.0%
!! TAATCAACTAAATTAACAAG+TGG - tig0032222:7890-7909 None:intergenic 20.0%
!! AATCAATTCAAAAAATGCAA+AGG + tig0032222:8136-8155 Msa1448550:CDS 20.0%
!!! GGAAAAAAATATTTTACTCT+AGG + tig0032222:8161-8180 Msa1448550:CDS 20.0%
!!! CTAAAATATCTAGATTTTGT+TGG - tig0032222:8347-8366 None:intergenic 20.0%
!!! TAGTCAATTTTATTATGTGA+TGG + tig0032222:8601-8620 Msa1448550:CDS 20.0%
!! GTTGAAAGATAAAATTGAAA+AGG + tig0032222:9306-9325 Msa1448550:CDS 20.0%
!!! AAATTCTTTGAATTTTGCAT+TGG - tig0032222:9419-9438 None:intergenic 20.0%
!! TTGTGTTATAGAAAGTAAAA+AGG + tig0032222:9510-9529 Msa1448550:CDS 20.0%
!! TGTAAAATTTTCTGCATTAG+TGG + tig0032222:7926-7945 Msa1448550:intron 25.0%
! AATTCAAAAAATGCAAAGGA+TGG + tig0032222:8140-8159 Msa1448550:CDS 25.0%
! TTAGATATTAATGATCAAGC+TGG + tig0032222:8404-8423 Msa1448550:CDS 25.0%
! GGATATGATAATCTTACATT+TGG + tig0032222:8473-8492 Msa1448550:CDS 25.0%
!!! ATTTTATTATGTGATGGATG+TGG + tig0032222:8607-8626 Msa1448550:CDS 25.0%
! TTTGTGGCATTTAATGAAAA+AGG + tig0032222:8967-8986 Msa1448550:CDS 25.0%
!! ATGATAATGAATGGTTGAAA+GGG + tig0032222:9122-9141 Msa1448550:CDS 25.0%
! AGTATGAATTCATTCTTTGA+TGG + tig0032222:9229-9248 Msa1448550:CDS 25.0%
! GTATGAATTCATTCTTTGAT+GGG + tig0032222:9230-9249 Msa1448550:CDS 25.0%
! TAAAATTGAAAAGGAAAGCA+TGG + tig0032222:9315-9334 Msa1448550:CDS 25.0%
!!! ATTGCTTGTATTAGTCTTTT+TGG + tig0032222:9364-9383 Msa1448550:CDS 25.0%
! ATGATAGATTTAAAGACACA+AGG + tig0032222:9536-9555 Msa1448550:intron 25.0%
! CTTGACAAAATATAACAAGA+CGG - tig0032222:9688-9707 None:intergenic 25.0%
GCGGTAGAAATTCTATAAAT+AGG + tig0032222:7251-7270 Msa1448550:intron 30.0%
TCAAAGTAAAGTGATGTTTG+AGG - tig0032222:7772-7791 None:intergenic 30.0%
TGGAATTGAATGTTGAAACA+GGG - tig0032222:7798-7817 None:intergenic 30.0%
! ATTTTCTGCATTAGTGGAAA+CGG + tig0032222:7932-7951 Msa1448550:intron 30.0%
AGCATAGCTCTTGTAATATT+TGG - tig0032222:8090-8109 None:intergenic 30.0%
AATAGGTGTATGTCTTTAGA+TGG + tig0032222:8272-8291 Msa1448550:CDS 30.0%
ATTTAGACCCTCATACAAAA+AGG + tig0032222:8378-8397 Msa1448550:CDS 30.0%
ATTACATTGACAAAGCAAGA+CGG + tig0032222:8513-8532 Msa1448550:CDS 30.0%
!! TGTTTTGAACAACCAAGAAA+AGG - tig0032222:8837-8856 None:intergenic 30.0%
TTTCTTGGTTGTTCAAAACA+TGG + tig0032222:8837-8856 Msa1448550:CDS 30.0%
TCTATCTTGGTCAGTAATTA+TGG - tig0032222:8894-8913 None:intergenic 30.0%
GCATTTAATGAAAAAGGTTC+CGG + tig0032222:8973-8992 Msa1448550:CDS 30.0%
! ATGAACTGCATGATAATGAA+TGG + tig0032222:9113-9132 Msa1448550:CDS 30.0%
! CATGATAATGAATGGTTGAA+AGG + tig0032222:9121-9140 Msa1448550:CDS 30.0%
! TGTATGTTAGAGACACATTT+TGG + tig0032222:9176-9195 Msa1448550:CDS 30.0%
! GTATGTTAGAGACACATTTT+GGG + tig0032222:9177-9196 Msa1448550:CDS 30.0%
!! TTGCATTGGTAAATGCTTTT+TGG - tig0032222:9405-9424 None:intergenic 30.0%
TACTGTAATGCTTTCTTAGA+GGG + tig0032222:9463-9482 Msa1448550:CDS 30.0%
ATTTAAAGACACAAGGTTCA+GGG + tig0032222:9543-9562 Msa1448550:intron 30.0%
! TGCTGCTTATTTGAGTTTAA+AGG + tig0032222:9604-9623 Msa1448550:intron 30.0%
!! CGTCTTGTTATATTTTGTCA+AGG + tig0032222:9686-9705 Msa1448550:CDS 30.0%
!! CTTGTTATATTTTGTCAAGG+TGG + tig0032222:9689-9708 Msa1448550:CDS 30.0%
TGAGATCAATCAATTAGTGG+CGG - tig0032222:7623-7642 None:intergenic 35.0%
TGGTGAGATCAATCAATTAG+TGG - tig0032222:7626-7645 None:intergenic 35.0%
GATATGCAGTTATAGTTGTG+CGG - tig0032222:7733-7752 None:intergenic 35.0%
AGTAAAGTGATGTTTGAGGA+TGG - tig0032222:7768-7787 None:intergenic 35.0%
GGAATTGAATGTTGAAACAG+GGG - tig0032222:7797-7816 None:intergenic 35.0%
GTGGAATTGAATGTTGAAAC+AGG - tig0032222:7799-7818 None:intergenic 35.0%
! TTTTCTGCATTAGTGGAAAC+GGG + tig0032222:7933-7952 Msa1448550:intron 35.0%
! ATCTACTTCTTTTGTCCAAG+AGG + tig0032222:7956-7975 Msa1448550:CDS 35.0%
GAAGCAAATATTGTTCAAGC+AGG + tig0032222:8017-8036 Msa1448550:CDS 35.0%
AAATATTGTTCAAGCAGGTG+AGG + tig0032222:8022-8041 Msa1448550:CDS 35.0%
AAGAGCTATGCTAGATGTAT+GGG + tig0032222:8098-8117 Msa1448550:CDS 35.0%
! TATGCTAGATGTATGGGTTT+TGG + tig0032222:8104-8123 Msa1448550:CDS 35.0%
! TGGTAGGATTTGGTTTCAAA+AGG - tig0032222:8226-8245 None:intergenic 35.0%
AGTGTATGGCTAGAGTAAAT+AGG + tig0032222:8255-8274 Msa1448550:CDS 35.0%
! CTAACCTCCTTTTTGTATGA+GGG - tig0032222:8388-8407 None:intergenic 35.0%
! TCTAACCTCCTTTTTGTATG+AGG - tig0032222:8389-8408 None:intergenic 35.0%
AAGCAGAAATTTCCGATCTA+TGG + tig0032222:8436-8455 Msa1448550:CDS 35.0%
TTGCTTCAACAACCATAGAT+CGG - tig0032222:8451-8470 None:intergenic 35.0%
TCTATGGTTGTTGAAGCAAA+TGG + tig0032222:8452-8471 Msa1448550:CDS 35.0%
TCGTTTGCGAAATGTATTCT+GGG + tig0032222:8640-8659 Msa1448550:CDS 35.0%
! ATGATTGACTCCAACAAAAG+GGG - tig0032222:8759-8778 None:intergenic 35.0%
! GATGATTGACTCCAACAAAA+GGG - tig0032222:8760-8779 None:intergenic 35.0%
! TGATGATTGACTCCAACAAA+AGG - tig0032222:8761-8780 None:intergenic 35.0%
TTTGTTGGAGTCAATCATCA+CGG + tig0032222:8761-8780 Msa1448550:CDS 35.0%
TGGTCAGTAATTATGGCATT+TGG - tig0032222:8887-8906 None:intergenic 35.0%
AAAAAGGTTCCGGAAAAGTT+GGG + tig0032222:8983-9002 Msa1448550:CDS 35.0%
! GAATAACTACCCAACTTTTC+CGG - tig0032222:8995-9014 None:intergenic 35.0%
TTCGAGCATATCTGATTTCA+TGG + tig0032222:9063-9082 Msa1448550:CDS 35.0%
TATCTGATTTCATGGAGAAG+TGG + tig0032222:9071-9090 Msa1448550:CDS 35.0%
TATTTGATGAGAGAGATCGT+TGG + tig0032222:9146-9165 Msa1448550:CDS 35.0%
ATTTGATGAGAGAGATCGTT+GGG + tig0032222:9147-9166 Msa1448550:CDS 35.0%
AATGTGTCTCTAACATACAC+AGG - tig0032222:9175-9194 None:intergenic 35.0%
GTACTGTAATGCTTTCTTAG+AGG + tig0032222:9462-9481 Msa1448550:CDS 35.0%
TAATGCTTTCTTAGAGGGAA+TGG + tig0032222:9468-9487 Msa1448550:CDS 35.0%
GATTTAAAGACACAAGGTTC+AGG + tig0032222:9542-9561 Msa1448550:intron 35.0%
! GTTATATTTTGTCAAGGTGG+AGG + tig0032222:9692-9711 Msa1448550:CDS 35.0%
AGTGGCGGTTAAATTGCATT+TGG - tig0032222:7608-7627 None:intergenic 40.0%
ACAAAGGTCTGTAGAATTGG+TGG - tig0032222:7646-7665 None:intergenic 40.0%
ACGACAAAGGTCTGTAGAAT+TGG - tig0032222:7649-7668 None:intergenic 40.0%
! TACTTCTTTTGTCCAAGAGG+AGG + tig0032222:7959-7978 Msa1448550:CDS 40.0%
TTATGTGATCAACCTCCTCT+TGG - tig0032222:7974-7993 None:intergenic 40.0%
CTAAATGTCATCCCAATCCT+AGG - tig0032222:8050-8069 None:intergenic 40.0%
CAAGAGCTATGCTAGATGTA+TGG + tig0032222:8097-8116 Msa1448550:CDS 40.0%
TGTGCTCTTATGGTAGGATT+TGG - tig0032222:8236-8255 None:intergenic 40.0%
ATACACTGTGCTCTTATGGT+AGG - tig0032222:8242-8261 None:intergenic 40.0%
! TACCATAAGAGCACAGTGTA+TGG + tig0032222:8241-8260 Msa1448550:CDS 40.0%
GTTGCTCTTGAGCATAATCA+TGG + tig0032222:8314-8333 Msa1448550:CDS 40.0%
TAGACCCTCATACAAAAAGG+AGG + tig0032222:8381-8400 Msa1448550:CDS 40.0%
GTCGTTTGCGAAATGTATTC+TGG + tig0032222:8639-8658 Msa1448550:CDS 40.0%
! AGGGCTGCTTATGAGTATTT+TGG + tig0032222:8677-8696 Msa1448550:CDS 40.0%
!!! CACGGTCAATCAGTTTTGTT+AGG + tig0032222:8779-8798 Msa1448550:CDS 40.0%
CGTATCCTCATTCGACAATA+GGG - tig0032222:8810-8829 None:intergenic 40.0%
CCTATTGTCGAATGAGGATA+CGG + tig0032222:8808-8827 Msa1448550:CDS 40.0%
! AGGATACGGAAACCTTTTCT+TGG + tig0032222:8822-8841 Msa1448550:CDS 40.0%
ACATGGTTAGAATGCATGCA+TGG + tig0032222:8854-8873 Msa1448550:CDS 40.0%
CCTTCTTCATTGCTCTATCT+TGG - tig0032222:8907-8926 None:intergenic 40.0%
CCAAGATAGAGCAATGAAGA+AGG + tig0032222:8904-8923 Msa1448550:CDS 40.0%
AGCAATGAAGAAGGCAATTG+AGG + tig0032222:8913-8932 Msa1448550:CDS 40.0%
GAAAAAGGTTCCGGAAAAGT+TGG + tig0032222:8982-9001 Msa1448550:CDS 40.0%
! TATTTTGTCAAGGTGGAGGA+AGG + tig0032222:9696-9715 Msa1448550:CDS 40.0%
AGCCATACACTGTGCTCTTA+TGG - tig0032222:8246-8265 None:intergenic 45.0%
! ATGCCTTTTGCCCCTTTTGT+TGG + tig0032222:8746-8765 Msa1448550:CDS 45.0%
ACTCCAACAAAAGGGGCAAA+AGG - tig0032222:8752-8771 None:intergenic 45.0%
TTGTGCCCTATTGTCGAATG+AGG + tig0032222:8802-8821 Msa1448550:CDS 45.0%
CCGTATCCTCATTCGACAAT+AGG - tig0032222:8811-8830 None:intergenic 45.0%
GGTTAGAATGCATGCATGGA+CGG + tig0032222:8858-8877 Msa1448550:CDS 45.0%
GTTAGAATGCATGCATGGAC+GGG + tig0032222:8859-8878 Msa1448550:CDS 45.0%
AATGCCACAAACACCATCGA+TGG - tig0032222:8958-8977 None:intergenic 45.0%
! GTTAGAGACACATTTTGGGC+TGG + tig0032222:9181-9200 Msa1448550:CDS 45.0%
CTGTGAGCTGAAGAACACAA+AGG - tig0032222:9648-9667 None:intergenic 45.0%
CTTTGTGTTCTTCAGCTCAC+AGG + tig0032222:9646-9665 Msa1448550:intron 45.0%
GGTGGAGGAAGGATATTAAG+CGG + tig0032222:9707-9726 Msa1448550:CDS 45.0%
GCATTTGGCGCTGTAAACGT+GGG - tig0032222:7593-7612 None:intergenic 50.0%
TGCATTTGGCGCTGTAAACG+TGG - tig0032222:7594-7613 None:intergenic 50.0%
GGTGAATACAATAGCGGCGA+TGG - tig0032222:7697-7716 None:intergenic 50.0%
CGTGGCGGTGAATACAATAG+CGG - tig0032222:7703-7722 None:intergenic 50.0%
CGCTATTGTATTCACCGCCA+CGG + tig0032222:7701-7720 Msa1448550:CDS 50.0%
TTCAAGCAGGTGAGGAACCT+AGG + tig0032222:8030-8049 Msa1448550:CDS 50.0%
CAGGTGAGGAACCTAGGATT+GGG + tig0032222:8036-8055 Msa1448550:CDS 50.0%
AAAGCAAGACGGCTTCGACT+TGG + tig0032222:8524-8543 Msa1448550:CDS 50.0%
AAGCAAGACGGCTTCGACTT+GGG + tig0032222:8525-8544 Msa1448550:CDS 50.0%
TCTGGGTAGATGCGAGATGT+AGG + tig0032222:8657-8676 Msa1448550:CDS 50.0%
CTGGGTAGATGCGAGATGTA+GGG + tig0032222:8658-8677 Msa1448550:CDS 50.0%
CAACAGCGACGAGACGACAA+AGG - tig0032222:7662-7681 None:intergenic 55.0%
GCAGGTGAGGAACCTAGGAT+TGG + tig0032222:8035-8054 Msa1448550:CDS 55.0%
!! AGACGGCTTCGACTTGGGAT+AGG + tig0032222:8530-8549 Msa1448550:CDS 55.0%
TTCCGAAAGCTCGCCATCGA+TGG + tig0032222:8942-8961 Msa1448550:CDS 55.0%
CACCATCGATGGCGAGCTTT+CGG - tig0032222:8947-8966 None:intergenic 55.0%
CTCGCCATCGATGGTGTTTG+TGG + tig0032222:8951-8970 Msa1448550:CDS 55.0%
TAGTTGTGCGGCGAGAACCG+TGG - tig0032222:7721-7740 None:intergenic 60.0%
TTGTGCGGCGAGAACCGTGG+CGG - tig0032222:7718-7737 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0032222 gene 7242 9759 7242 ID=Msa1448550;Name=Msa1448550
tig0032222 mRNA 7242 9759 7242 ID=Msa1448550-mRNA-1;Parent=Msa1448550;Name=Msa1448550-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|0.25|1|1|4|0|619
tig0032222 exon 7242 7253 7242 ID=Msa1448550-mRNA-1:exon:16453;Parent=Msa1448550-mRNA-1
tig0032222 exon 7658 7820 7658 ID=Msa1448550-mRNA-1:exon:16454;Parent=Msa1448550-mRNA-1
tig0032222 exon 7946 9531 7946 ID=Msa1448550-mRNA-1:exon:16455;Parent=Msa1448550-mRNA-1
tig0032222 exon 9661 9759 9661 ID=Msa1448550-mRNA-1:exon:16456;Parent=Msa1448550-mRNA-1
tig0032222 CDS 7242 7253 7242 ID=Msa1448550-mRNA-1:cds;Parent=Msa1448550-mRNA-1
tig0032222 CDS 7658 7820 7658 ID=Msa1448550-mRNA-1:cds;Parent=Msa1448550-mRNA-1
tig0032222 CDS 7946 9531 7946 ID=Msa1448550-mRNA-1:cds;Parent=Msa1448550-mRNA-1
tig0032222 CDS 9661 9759 9661 ID=Msa1448550-mRNA-1:cds;Parent=Msa1448550-mRNA-1
Gene Sequence

>Msa1448550

ATGAATCATGCGACCTTTGTCGTCTCGTCGCTGTTGTTGTCTGTTCTACCATCGCCGCTATTGTATTCACCGCCACGGTTCTCGCCGCACAACTATAACTGCATATCGTCGCTGCATCTCCATCCTCAAACATCACTTTACTTTGATTCCCCTGTTTCAACATTCAATTCCACAATGGAAACGGGATCTACTTCTTTTGTCCAAGAGGAGGTTGATCACATAAATGAAAACAGTGATGAAAATTTAGAAGCAAATATTGTTCAAGCAGGTGAGGAACCTAGGATTGGGATGACATTTAGTTCTGAAGAAGAAGTTACCAAATATTACAAGAGCTATGCTAGATGTATGGGTTTTGGAACTGTTAAAATCAATTCAAAAAATGCAAAGGATGGAAAAAAATATTTTACTCTAGGATGTACTCGTGCAAGAAGCTATGTGAGTAACTCAAAGAACCTTTTGAAACCAAATCCTACCATAAGAGCACAGTGTATGGCTAGAGTAAATAGGTGTATGTCTTTAGATGGAAAAATTACAATTACAAAAGTTGCTCTTGAGCATAATCATGGATTAAGCCCAACAAAATCTAGATATTTTAGATGCAATAAGAATTTAGACCCTCATACAAAAAGGAGGTTAGATATTAATGATCAAGCTGGTATTAATGTAAGCAGAAATTTCCGATCTATGGTTGTTGAAGCAAATGGATATGATAATCTTACATTTGGAGAAAAAGATTGTAGAAATTACATTGACAAAGCAAGACGGCTTCGACTTGGGATAGGAGATGCTGAAGCTATTCAAAACTATTTTGTCAGAATGCAAAAGAAAAATAGTCAATTTTATTATGTGATGGATGTGGATGACAAAAGTCGTTTGCGAAATGTATTCTGGGTAGATGCGAGATGTAGGGCTGCTTATGAGTATTTTGGTGAAGTCATAACTTTTGATACCACTTATTTAACAAATAAATATGACATGCCTTTTGCCCCTTTTGTTGGAGTCAATCATCACGGTCAATCAGTTTTGTTAGGTTGTGCCCTATTGTCGAATGAGGATACGGAAACCTTTTCTTGGTTGTTCAAAACATGGTTAGAATGCATGCATGGACGGGCACCAAATGCCATAATTACTGACCAAGATAGAGCAATGAAGAAGGCAATTGAGGTCGTCTTTCCGAAAGCTCGCCATCGATGGTGTTTGTGGCATTTAATGAAAAAGGTTCCGGAAAAGTTGGGTAGTTATTCTCATTATGAGTCTATCAAATTACTTTTGCATGATGTTGTGTATGATTCTTCGAGCATATCTGATTTCATGGAGAAGTGGAAAAAAATGATTGAATGTTATGAACTGCATGATAATGAATGGTTGAAAGGGCTATTTGATGAGAGAGATCGTTGGGTTCCTGTGTATGTTAGAGACACATTTTGGGCTGGAATGTCAACTACACAACGAAGTGAAAGTATGAATTCATTCTTTGATGGGTATGTGAGCTCAAAGACAACATTGAAGCAATTTGTTGAACAATATGACAATGCGTTGAAAGATAAAATTGAAAAGGAAAGCATGGCTGATTTTGTTTCATTTAATACAACAATTGCTTGTATTAGTCTTTTTGGCTTTGAGTCTCAATTCCAAAAAGCATTTACCAATGCAAAATTCAAAGAATTTCAAATTGAAATTGCTTCTATGATGTACTGTAATGCTTTCTTAGAGGGAATGGAGAATTTGAATTCAACATTTTGTGTTATAGAAAGTAAAAAGCTCACAGGTAAAACTGAGTCAGTGCCGTCTTGTTATATTTTGTCAAGGTGGAGGAAGGATATTAAGCGGAGACACACGCTTATTAAATGTGAGAGTTGA
Protein sequence

>Msa1448550

MNHATFVVSSLLLSVLPSPLLYSPPRFSPHNYNCISSLHLHPQTSLYFDSPVSTFNSTMETGSTSFVQEEVDHINENSDENLEANIVQAGEEPRIGMTFSSEEEVTKYYKSYARCMGFGTVKINSKNAKDGKKYFTLGCTRARSYVSNSKNLLKPNPTIRAQCMARVNRCMSLDGKITITKVALEHNHGLSPTKSRYFRCNKNLDPHTKRRLDINDQAGINVSRNFRSMVVEANGYDNLTFGEKDCRNYIDKARRLRLGIGDAEAIQNYFVRMQKKNSQFYYVMDVDDKSRLRNVFWVDARCRAAYEYFGEVITFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSNEDTETFSWLFKTWLECMHGRAPNAIITDQDRAMKKAIEVVFPKARHRWCLWHLMKKVPEKLGSYSHYESIKLLLHDVVYDSSSISDFMEKWKKMIECYELHDNEWLKGLFDERDRWVPVYVRDTFWAGMSTTQRSESMNSFFDGYVSSKTTLKQFVEQYDNALKDKIEKESMADFVSFNTTIACISLFGFESQFQKAFTNAKFKEFQIEIASMMYCNAFLEGMENLNSTFCVIESKKLTGKTESVPSCYILSRWRKDIKRRHTLIKCES*