AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449610 MtrunA17_Chr7g0274141 59.375 384 129 10 2 368 12 385 1.42e-117 346
Msa1449610 MtrunA17_Chr1g0190761 49.211 317 132 11 65 359 82 391 2.73e-70 226
Msa1449610 MtrunA17_Chr3g0139301 36.296 135 77 4 128 256 76 207 1.10e-12 68.2
Msa1449610 MtrunA17_Chr1g0147491 42.500 120 66 2 147 265 142 259 2.73e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449610 AT5G28770.2 38.346 266 114 9 14 268 20 246 7.15e-29 114
Msa1449610 AT5G28770.1 36.981 265 112 8 14 268 20 239 8.07e-27 108
Msa1449610 AT4G02640.3 39.301 229 113 9 44 264 102 312 7.18e-23 99.8
Msa1449610 AT4G02640.2 39.301 229 113 9 44 264 102 312 1.08e-22 99.0
Msa1449610 AT4G02640.4 39.381 226 111 8 44 264 102 306 1.76e-22 98.6
Msa1449610 AT4G02640.1 39.381 226 111 8 44 264 102 306 1.88e-22 98.2
Msa1449610 AT5G28770.3 37.143 245 104 9 14 247 20 225 9.09e-22 93.6
Msa1449610 AT5G24800.1 42.069 145 66 5 130 261 71 210 5.99e-21 92.0
Msa1449610 AT3G54620.1 30.189 318 139 9 18 279 40 330 2.97e-19 89.0

Find 79 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCAGGATGCTCCAAAAT+AGG 0.198060 tig0032655:-31654 Msa1449610:intron
CGACTTCAGCCTACTGTAAA+AGG 0.200539 tig0032655:-32059 Msa1449610:intron
GTTTAGCTGATTCCCTATTT+TGG 0.286405 tig0032655:+31641 None:intergenic
GGATCTGATCCTTCTGATAT+TGG 0.302870 tig0032655:-32110 Msa1449610:CDS
CCCTTCTGAATTACAAAATA+AGG 0.310861 tig0032655:-31900 Msa1449610:CDS
GAGAGATGTAAAAGATTAAC+TGG 0.323266 tig0032655:-31039 Msa1449610:CDS
TGTCTAAAGGAAATTGATAA+AGG 0.341441 tig0032655:-34122 Msa1449610:CDS
GGAGAGGTTAATATGAATAT+TGG 0.345096 tig0032655:-31773 Msa1449610:CDS
ACAAAGGTGGAAGAAGTTAA+AGG 0.357578 tig0032655:+34071 None:intergenic
AGGGGAAGAGAAAGCTTCTC+AGG 0.359196 tig0032655:-31795 Msa1449610:CDS
GATATGAGAGGAGTCAATAA+TGG 0.367652 tig0032655:-30847 Msa1449610:CDS
TATTAGGTCTCTACATTAAG+AGG 0.369226 tig0032655:-31509 Msa1449610:intron
GTTCTATCACTTGTCTTCTT+CGG 0.371352 tig0032655:+30685 None:intergenic
AAGATTAACTGGATCAATTC+CGG 0.379673 tig0032655:-31028 Msa1449610:CDS
GGTGCATTCAAGATCAAGTT+TGG 0.383838 tig0032655:+33977 None:intergenic
ATTGATGTTGCCTCAGACAT+TGG 0.396102 tig0032655:+30991 None:intergenic
TTTCAGGATGCTCCAAAATA+GGG 0.416451 tig0032655:-31653 Msa1449610:intron
GACATTGGATTATTAAAAGT+TGG 0.427671 tig0032655:+30877 None:intergenic
ACATACCCAAGTCATAATAA+AGG 0.434312 tig0032655:+33953 None:intergenic
AGAGGAGTCAATAATGGGTT+GGG 0.448583 tig0032655:-30841 Msa1449610:CDS
GCAGCAGGATGGAACGAGAT+AGG 0.450336 tig0032655:-30778 Msa1449610:CDS
TGATGACCAGGCTCTTCTTA+AGG 0.454270 tig0032655:-34000 Msa1449610:CDS
CAAAGCTATCCACCCGCGGC+AGG 0.455051 tig0032655:+30743 None:intergenic
TGAAACCTGTTGTCACAATC+AGG 0.455873 tig0032655:-31838 Msa1449610:CDS
GACATGATCATAGTTGCTGC+TGG 0.457615 tig0032655:-31878 Msa1449610:CDS
ATGGGAATGCCATTACTTGA+TGG 0.462778 tig0032655:-30970 Msa1449610:CDS
AATGTCTGAGGCAACATCAA+TGG 0.467879 tig0032655:-30989 Msa1449610:CDS
GTCTAAAGGAAATTGATAAA+GGG 0.468058 tig0032655:-34121 Msa1449610:CDS
GAGAGGAGTCAATAATGGGT+TGG 0.473171 tig0032655:-30842 Msa1449610:CDS
AAGGAGAAACAAAGATGAAT+CGG 0.475504 tig0032655:-34172 Msa1449610:CDS
ATGTCTGAGGCAACATCAAT+GGG 0.480461 tig0032655:-30988 Msa1449610:CDS
TCAACGACAAGTGGATCCTC+AGG 0.483871 tig0032655:-31815 Msa1449610:CDS
ATATGAGAGGAGTCAATAAT+GGG 0.486438 tig0032655:-30846 Msa1449610:CDS
GATATTGGACCACAGACATC+TGG 0.488242 tig0032655:-32095 Msa1449610:CDS
GCACCGCCTTTATTATGACT+TGG 0.488592 tig0032655:-33959 Msa1449610:CDS
CACCGCCTTTATTATGACTT+GGG 0.491230 tig0032655:-33958 Msa1449610:intron
TTATTTGGATCTTCTTGCAC+AGG 0.496736 tig0032655:+30913 None:intergenic
TTTGAGATTGTTATTAACAA+AGG 0.498642 tig0032655:+34055 None:intergenic
GTTGACCTGATTGTGACAAC+AGG 0.513826 tig0032655:+31833 None:intergenic
GGATAGCTTTGATCATCAGC+TGG 0.514472 tig0032655:-30731 Msa1449610:CDS
ACAGGTTTCAGTGGCATGTA+GGG 0.514510 tig0032655:+31851 None:intergenic
GTGCAGAAGAACACTGCAGC+AGG 0.518310 tig0032655:-30793 Msa1449610:CDS
AACAAATTCCCTGCCGCGGG+TGG 0.522410 tig0032655:-30752 Msa1449610:CDS
ATTGGCATTGCATTAAATGC+CGG 0.535991 tig0032655:+31009 None:intergenic
GACTCCTCTCATATCATGAC+TGG 0.537853 tig0032655:+30855 None:intergenic
CTCATATCATGACTGGACAT+TGG 0.537888 tig0032655:+30862 None:intergenic
ATGTCGGATGCGAGGAACGA+AGG 0.545841 tig0032655:-34191 Msa1449610:CDS
GAGATTGTTATTAACAAAGG+TGG 0.550741 tig0032655:+34058 None:intergenic
ACATGATCATAGTTGCTGCT+GGG 0.551656 tig0032655:-31877 Msa1449610:CDS
GTTTGGCCTTAAGAAGAGCC+TGG 0.554569 tig0032655:+33994 None:intergenic
CTGATGCAAAACGAGTTAAG+AGG 0.557366 tig0032655:-31739 Msa1449610:intron
TTTAGAGAAAGATGATGACC+AGG 0.565934 tig0032655:-34012 Msa1449610:CDS
AGAGAAAGCTTCTCAGGGAG+AGG 0.567096 tig0032655:-31789 Msa1449610:CDS
AAGAACAAATTCCCTGCCGC+GGG 0.569746 tig0032655:-30755 Msa1449610:CDS
AACAGGTTTCAGTGGCATGT+AGG 0.571055 tig0032655:+31850 None:intergenic
ACAATCAGGTCAACGACAAG+TGG 0.581189 tig0032655:-31824 Msa1449610:CDS
GTCTGTGGTCCAATATCAGA+AGG 0.586487 tig0032655:+32101 None:intergenic
GGAACAATATTTGTTGATGT+CGG 0.586597 tig0032655:-34207 Msa1449610:CDS
GAAGGACTTCCATCAAGTAA+TGG 0.591255 tig0032655:+30961 None:intergenic
TAATGCAATGCCAATGTCTG+AGG 0.599128 tig0032655:-31001 Msa1449610:CDS
AAGGTGGAAGAAGTTAAAGG+TGG 0.599798 tig0032655:+34074 None:intergenic
AAAGCTATCCACCCGCGGCA+GGG 0.601789 tig0032655:+30744 None:intergenic
GGGGAAGAGAAAGCTTCTCA+GGG 0.602774 tig0032655:-31794 Msa1449610:CDS
ATTTGTTGATGTCGGATGCG+AGG 0.603239 tig0032655:-34199 Msa1449610:CDS
CATGATCATAGTTGCTGCTG+GGG 0.605839 tig0032655:-31876 Msa1449610:CDS
GGCTGAAGTCGAAGTTCTGA+AGG 0.612129 tig0032655:+32071 None:intergenic
AAATACTAAGCTTGAAACAC+AGG 0.618412 tig0032655:-31593 Msa1449610:intron
GCATCAGCTGATAACTCAGA+AGG 0.620964 tig0032655:+30943 None:intergenic
CAACGACAAGTGGATCCTCA+GGG 0.625807 tig0032655:-31814 Msa1449610:CDS
ATTGTGACAACAGGTTTCAG+TGG 0.628600 tig0032655:+31842 None:intergenic
GGAGTCAATAATGGGTTGGG+AGG 0.632977 tig0032655:-30838 Msa1449610:CDS
GAAGAACAAATTCCCTGCCG+CGG 0.646610 tig0032655:-30756 Msa1449610:CDS
AGAAGCTTTCTCTTCCCCTG+AGG 0.650082 tig0032655:+31799 None:intergenic
ATGTCCAGTCATGATATGAG+AGG 0.664118 tig0032655:-30859 Msa1449610:CDS
AGAAGAACACTGCAGCAGGA+TGG 0.669756 tig0032655:-30789 Msa1449610:CDS
TACCCAAGTCATAATAAAGG+CGG 0.681827 tig0032655:+33956 None:intergenic
TCTGAAGGACCAGATGTCTG+TGG 0.689035 tig0032655:+32086 None:intergenic
AACGACAAGTGGATCCTCAG+GGG 0.710138 tig0032655:-31813 Msa1449610:CDS
TGATCAAAGCTATCCACCCG+CGG 0.714097 tig0032655:+30739 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTAAACTAAAATAGTT+AGG + tig0032655:31272-31291 None:intergenic 10.0%
!!! AATTAAAACTGAAAAATTTT+CGG - tig0032655:31606-31625 Msa1449610:CDS 10.0%
!! AATTAATTATAAATCATCTT+GGG - tig0032655:31918-31937 Msa1449610:intron 10.0%
!!! TTTAAAACATAATAATAAGT+TGG + tig0032655:32124-32143 None:intergenic 10.0%
!!! ACAATTTTTTTTAATCAAAG+TGG + tig0032655:30990-31009 None:intergenic 15.0%
!!! ATTAAAACTGAAAAATTTTC+GGG - tig0032655:31607-31626 Msa1449610:CDS 15.0%
!!! AAACTGAATTTTTTTTTTGA+AGG - tig0032655:31633-31652 Msa1449610:CDS 15.0%
!! ACAAAAAAATAAAATAAGTC+AGG - tig0032655:31663-31682 Msa1449610:intron 15.0%
!! CAAAAAAATAAAATAAGTCA+GGG - tig0032655:31664-31683 Msa1449610:intron 15.0%
!! AAAAAAATAAAATAAGTCAG+GGG - tig0032655:31665-31684 Msa1449610:intron 15.0%
!! GAGCATATATTATATATTAT+AGG + tig0032655:31866-31885 None:intergenic 15.0%
!! GATGATTTATAATTAATTGA+CGG + tig0032655:31916-31935 None:intergenic 15.0%
!! CAATTAATTATAAATCATCT+TGG - tig0032655:31917-31936 Msa1449610:intron 15.0%
!!! CTTGATAATAAATAATAAGT+TGG + tig0032655:32156-32175 None:intergenic 15.0%
!! ACATATATAATTTCTCTTTA+TGG - tig0032655:32432-32451 Msa1449610:intron 15.0%
!!! TTTGAGATTGTTATTAACAA+AGG + tig0032655:30835-30854 None:intergenic 20.0%
!!! ATTTTATGTGTATGATAATC+AGG - tig0032655:31051-31070 Msa1449610:CDS 20.0%
!! AATATTGTTTAAGAAATCTG+AGG - tig0032655:31953-31972 Msa1449610:intron 20.0%
!! ACCTATATATGAAAAAGAAT+GGG + tig0032655:32322-32341 None:intergenic 20.0%
!!! TTCCTATATGTTTTGAATTA+TGG - tig0032655:32405-32424 Msa1449610:intron 20.0%
!! TTCCATAATTCAAAACATAT+AGG + tig0032655:32410-32429 None:intergenic 20.0%
!! TAAGCCAAAAATAAAATATG+AGG + tig0032655:32676-32695 None:intergenic 20.0%
!! CAATTATATTCTTCAAAGTT+TGG - tig0032655:32705-32724 Msa1449610:intron 20.0%
!!! TTTTCTTGTTCTAATATTCA+TGG - tig0032655:32748-32767 Msa1449610:intron 20.0%
!! TTCTAATATTCATGGAAATA+GGG - tig0032655:32756-32775 Msa1449610:intron 20.0%
!! TTAAATTGTTTATCCAAACA+TGG - tig0032655:33188-33207 Msa1449610:intron 20.0%
!! ACATGATATTCTATCTTATT+AGG - tig0032655:33362-33381 Msa1449610:intron 20.0%
!! ACACATATATCAATTGAAAA+TGG + tig0032655:33781-33800 None:intergenic 20.0%
!! GGTTGAAAAAAGAAATTATT+TGG + tig0032655:33992-34011 None:intergenic 20.0%
! TGTCTAAAGGAAATTGATAA+AGG - tig0032655:30765-30784 Msa1449610:CDS 25.0%
! GTCTAAAGGAAATTGATAAA+GGG - tig0032655:30766-30785 Msa1449610:CDS 25.0%
!!! ATCAGGTTTTATGAGAATAA+AGG - tig0032655:31068-31087 Msa1449610:intron 25.0%
! GAGATAGTACTATCATATTA+CGG - tig0032655:31213-31232 Msa1449610:intron 25.0%
! TTAACTGATTAAACTTTGCA+GGG + tig0032655:31763-31782 None:intergenic 25.0%
! TTTAACTGATTAAACTTTGC+AGG + tig0032655:31762-31781 None:intergenic 25.0%
! TCAAATTAACAAAATGGCAT+GGG + tig0032655:31705-31724 None:intergenic 25.0%
! ATCAAATTAACAAAATGGCA+TGG + tig0032655:31706-31725 None:intergenic 25.0%
! ACGTCATCAAATTAACAAAA+TGG + tig0032655:31711-31730 None:intergenic 25.0%
!!! ATTTTGTTAATTTGATGACG+TGG - tig0032655:31710-31729 Msa1449610:intron 25.0%
! TTTAACTGATTAAACTTTGC+AGG - tig0032655:31762-31781 Msa1449610:CDS 25.0%
! TTAACTGATTAAACTTTGCA+GGG - tig0032655:31763-31782 Msa1449610:CDS 25.0%
!! CCTAATAGTTTTTCTACTTA+GGG + tig0032655:31893-31912 None:intergenic 25.0%
! TGTATATCATAAACACTTCA+TGG - tig0032655:31994-32013 Msa1449610:intron 25.0%
!! TAACATATTAGTTGATGTGT+CGG - tig0032655:32027-32046 Msa1449610:intron 25.0%
!!! TTTCATATCATGCTAGTTTT+TGG - tig0032655:32209-32228 Msa1449610:intron 25.0%
! CTAGAAGGTTAATTAGTTAA+AGG + tig0032655:32270-32289 None:intergenic 25.0%
! CCTATATATGAAAAAGAATG+GGG + tig0032655:32321-32340 None:intergenic 25.0%
! CACCTATATATGAAAAAGAA+TGG + tig0032655:32323-32342 None:intergenic 25.0%
!! TTTAAAGTCACACACATTAA+TGG + tig0032655:32511-32530 None:intergenic 25.0%
!! GTAAAGAGTTTTTATCTTGT+TGG + tig0032655:32590-32609 None:intergenic 25.0%
! TATACTTGAAAAGCAGTAAA+TGG + tig0032655:32642-32661 None:intergenic 25.0%
!!! CATGCCTCATATTTTATTTT+TGG - tig0032655:32669-32688 Msa1449610:intron 25.0%
! GTTCTAATATTCATGGAAAT+AGG - tig0032655:32755-32774 Msa1449610:intron 25.0%
! TGCAATTATGAAGTTTAACA+TGG - tig0032655:32875-32894 Msa1449610:intron 25.0%
! ATTTATTTATCTGTAGAGTG+TGG - tig0032655:32964-32983 Msa1449610:intron 25.0%
!!! CCTTATTTTGTAATTCAGAA+GGG + tig0032655:32990-33009 None:intergenic 25.0%
!!! TCCTTATTTTGTAATTCAGA+AGG + tig0032655:32991-33010 None:intergenic 25.0%
! TTAAGAGGTAACAATATTCT+AGG - tig0032655:33163-33182 Msa1449610:intron 25.0%
!!! TTTTGAAACAATAAAAGCGA+AGG - tig0032655:33488-33507 Msa1449610:intron 25.0%
! GACATTGGATTATTAAAAGT+TGG + tig0032655:34013-34032 None:intergenic 25.0%
! GGAACAATATTTGTTGATGT+CGG - tig0032655:30680-30699 Msa1449610:CDS 30.0%
AAGGAGAAACAAAGATGAAT+CGG - tig0032655:30715-30734 Msa1449610:CDS 30.0%
! GAGATTGTTATTAACAAAGG+TGG + tig0032655:30832-30851 None:intergenic 30.0%
ACATACCCAAGTCATAATAA+AGG + tig0032655:30937-30956 None:intergenic 30.0%
!! GGTTTTATGAGAATAAAGGT+AGG - tig0032655:31072-31091 Msa1449610:intron 30.0%
TTATGAGAATAAAGGTAGGT+AGG - tig0032655:31076-31095 Msa1449610:intron 30.0%
TATGAGAATAAAGGTAGGTA+GGG - tig0032655:31077-31096 Msa1449610:intron 30.0%
TAAAAAAAGCAACAGACTTG+TGG + tig0032655:31182-31201 None:intergenic 30.0%
TAACTGATTAAACTTTGCAG+GGG + tig0032655:31764-31783 None:intergenic 30.0%
!!! TTATGGTAATTTTGATGACG+TGG + tig0032655:31439-31458 None:intergenic 30.0%
CGTCATCAAAATTACCATAA+TGG - tig0032655:31439-31458 Msa1449610:CDS 30.0%
TCAAAATTACCATAATGGCA+TGG - tig0032655:31444-31463 Msa1449610:CDS 30.0%
CAAAATTACCATAATGGCAT+GGG - tig0032655:31445-31464 Msa1449610:CDS 30.0%
AAGGAAAAAAAATTAGCCCA+GGG + tig0032655:31486-31505 None:intergenic 30.0%
AAAGGAAAAAAAATTAGCCC+AGG + tig0032655:31487-31506 None:intergenic 30.0%
TAACTGATTAAACTTTGCAG+GGG - tig0032655:31764-31783 Msa1449610:CDS 30.0%
CCCTAAGTAGAAAAACTATT+AGG - tig0032655:31890-31909 Msa1449610:CDS 30.0%
! GCCTAATAGTTTTTCTACTT+AGG + tig0032655:31894-31913 None:intergenic 30.0%
ATATCATAAACACTTCATGG+CGG - tig0032655:31997-32016 Msa1449610:intron 30.0%
! ACAAAACAAGTTCAAGTTCA+AGG + tig0032655:32190-32209 None:intergenic 30.0%
!! GATTAAGAAGCATTACTAGA+AGG + tig0032655:32285-32304 None:intergenic 30.0%
CTAGTAATGCTTCTTAATCA+TGG - tig0032655:32286-32305 Msa1449610:intron 30.0%
!! CCCCATTCTTTTTCATATAT+AGG - tig0032655:32318-32337 Msa1449610:intron 30.0%
AAACATGTATAACGATGTGA+CGG - tig0032655:32550-32569 Msa1449610:intron 30.0%
! CAATTCAAACCTTTTACAGT+AGG + tig0032655:32840-32859 None:intergenic 30.0%
CCCTTCTGAATTACAAAATA+AGG - tig0032655:32987-33006 Msa1449610:intron 30.0%
GGAGAGGTTAATATGAATAT+TGG - tig0032655:33114-33133 Msa1449610:intron 30.0%
AAATACTAAGCTTGAAACAC+AGG - tig0032655:33294-33313 Msa1449610:intron 30.0%
TATTAGGTCTCTACATTAAG+AGG - tig0032655:33378-33397 Msa1449610:intron 30.0%
GTGACTACTTAGATTAAGAT+TGG - tig0032655:33625-33644 Msa1449610:intron 30.0%
!! TGCTTTGAAGAATCAAAATC+AGG - tig0032655:33669-33688 Msa1449610:intron 30.0%
GAGAGATGTAAAAGATTAAC+TGG - tig0032655:33848-33867 Msa1449610:intron 30.0%
AAGATTAACTGGATCAATTC+CGG - tig0032655:33859-33878 Msa1449610:intron 30.0%
ATATGAGAGGAGTCAATAAT+GGG - tig0032655:34041-34060 Msa1449610:CDS 30.0%
! AGATTTTCTGCAGTGTCTAA+AGG - tig0032655:30752-30771 Msa1449610:CDS 35.0%
ACAAAGGTGGAAGAAGTTAA+AGG + tig0032655:30819-30838 None:intergenic 35.0%
TTTAGAGAAAGATGATGACC+AGG - tig0032655:30875-30894 Msa1449610:CDS 35.0%
TACCCAAGTCATAATAAAGG+CGG + tig0032655:30934-30953 None:intergenic 35.0%
TAGTTAGGTTATGTGTGTTG+CGG + tig0032655:31257-31276 None:intergenic 35.0%
! AAAAAGGATTAGCGATTGCA+GGG + tig0032655:31791-31810 None:intergenic 35.0%
! AAAAAAGGATTAGCGATTGC+AGG + tig0032655:31790-31809 None:intergenic 35.0%
TTGCAGGGGCAAAAAAAAAA+AGG + tig0032655:31371-31390 None:intergenic 35.0%
AGGAAAAAAAATTAGCCCAG+GGG + tig0032655:31485-31504 None:intergenic 35.0%
! AAAAAAGGATTAGCGATTGC+AGG - tig0032655:31790-31809 Msa1449610:CDS 35.0%
! AAAAAGGATTAGCGATTGCA+GGG - tig0032655:31791-31810 Msa1449610:CDS 35.0%
TTATTTGTCCCTGCAAATAG+GGG + tig0032655:31833-31852 None:intergenic 35.0%
GTTATTTGTCCCTGCAAATA+GGG + tig0032655:31834-31853 None:intergenic 35.0%
! TGTTATTTGTCCCTGCAAAT+AGG + tig0032655:31835-31854 None:intergenic 35.0%
TTCTCTTTATGGCTAGACTT+TGG - tig0032655:32443-32462 Msa1449610:intron 35.0%
ACTTGAAAAGCAGTAAATGG+AGG + tig0032655:32639-32658 None:intergenic 35.0%
AGAAGTAGAACTCATTTGCA+TGG - tig0032655:32911-32930 Msa1449610:intron 35.0%
GAAGTAGAACTCATTTGCAT+GGG - tig0032655:32912-32931 Msa1449610:intron 35.0%
! TCTTAACTCGTTTTGCATCA+GGG + tig0032655:33149-33168 None:intergenic 35.0%
TTAGGCATTAAGACCATGTT+TGG + tig0032655:33204-33223 None:intergenic 35.0%
TTTCAGGATGCTCCAAAATA+GGG - tig0032655:33234-33253 Msa1449610:intron 35.0%
!! GTTTAGCTGATTCCCTATTT+TGG + tig0032655:33249-33268 None:intergenic 35.0%
GATCAATACTCTATGCAAAC+CGG - tig0032655:33567-33586 Msa1449610:intron 35.0%
! TATGCTTAACTAAGGCATTC+CGG + tig0032655:33589-33608 None:intergenic 35.0%
! ATCTGACATGCTCATTTTGA+AGG - tig0032655:33811-33830 Msa1449610:intron 35.0%
ATTGGCATTGCATTAAATGC+CGG + tig0032655:33881-33900 None:intergenic 35.0%
TTATTTGGATCTTCTTGCAC+AGG + tig0032655:33977-33996 None:intergenic 35.0%
GATATGAGAGGAGTCAATAA+TGG - tig0032655:34040-34059 Msa1449610:CDS 35.0%
GTTCTATCACTTGTCTTCTT+CGG + tig0032655:34205-34224 None:intergenic 35.0%
AAGGTGGAAGAAGTTAAAGG+TGG + tig0032655:30816-30835 None:intergenic 40.0%
GGTGCATTCAAGATCAAGTT+TGG + tig0032655:30913-30932 None:intergenic 40.0%
CACCGCCTTTATTATGACTT+GGG - tig0032655:30929-30948 Msa1449610:CDS 40.0%
AGAATAAAGGTAGGTAGGGT+TGG - tig0032655:31081-31100 Msa1449610:intron 40.0%
TGTTATTCGTCCCTGCAAAT+AGG - tig0032655:31311-31330 Msa1449610:intron 40.0%
GTTATTCGTCCCTGCAAATA+GGG - tig0032655:31312-31331 Msa1449610:intron 40.0%
TTATTCGTCCCTGCAAATAG+GGG - tig0032655:31313-31332 Msa1449610:intron 40.0%
GAATTGACGAAAATACCCCT+GGG - tig0032655:31467-31486 Msa1449610:CDS 40.0%
! GCAGTCATTAACTTGGTCAA+GGG - tig0032655:31732-31751 Msa1449610:intron 40.0%
ACTTTGCAGGGGCAAAAAAA+AGG - tig0032655:31775-31794 Msa1449610:CDS 40.0%
CTTTATGGCTAGACTTTGGA+AGG - tig0032655:32447-32466 Msa1449610:intron 40.0%
! ATGGTTGTGATGTGCTGATA+GGG + tig0032655:32492-32511 None:intergenic 40.0%
! AATGGTTGTGATGTGCTGAT+AGG + tig0032655:32493-32512 None:intergenic 40.0%
GGATCTGATCCTTCTGATAT+TGG - tig0032655:32777-32796 Msa1449610:intron 40.0%
ACATGATCATAGTTGCTGCT+GGG - tig0032655:33010-33029 Msa1449610:intron 40.0%
ATTGTGACAACAGGTTTCAG+TGG + tig0032655:33048-33067 None:intergenic 40.0%
TGAAACCTGTTGTCACAATC+AGG - tig0032655:33049-33068 Msa1449610:intron 40.0%
! CTCTTAACTCGTTTTGCATC+AGG + tig0032655:33150-33169 None:intergenic 40.0%
CTGATGCAAAACGAGTTAAG+AGG - tig0032655:33148-33167 Msa1449610:intron 40.0%
TCCTGAAAGGCAATGTCATT+AGG + tig0032655:33222-33241 None:intergenic 40.0%
!!! CTATTTTGGAGCATCCTGAA+AGG + tig0032655:33235-33254 None:intergenic 40.0%
CTTTCAGGATGCTCCAAAAT+AGG - tig0032655:33233-33252 Msa1449610:intron 40.0%
TGACTGCGTATGCTTAACTA+AGG + tig0032655:33597-33616 None:intergenic 40.0%
GGATGTTCAAGATTCATCCT+CGG - tig0032655:33646-33665 Msa1449610:intron 40.0%
!! TTTGATTCTTCAAAGCACCG+AGG + tig0032655:33666-33685 None:intergenic 40.0%
! GGTTGCTATTGGATTCAGAT+CGG + tig0032655:33712-33731 None:intergenic 40.0%
GAATGCAGCAATATTCCAAC+TGG - tig0032655:33748-33767 Msa1449610:intron 40.0%
! GAAAATGGATTCCTTCCAGT+TGG + tig0032655:33766-33785 None:intergenic 40.0%
!! GCTCATTTTGAAGGTGAATG+CGG - tig0032655:33820-33839 Msa1449610:intron 40.0%
TAATGCAATGCCAATGTCTG+AGG - tig0032655:33886-33905 Msa1449610:intron 40.0%
ATTGATGTTGCCTCAGACAT+TGG + tig0032655:33899-33918 None:intergenic 40.0%
! AATGTCTGAGGCAACATCAA+TGG - tig0032655:33898-33917 Msa1449610:intron 40.0%
! ATGTCTGAGGCAACATCAAT+GGG - tig0032655:33899-33918 Msa1449610:intron 40.0%
ATGGGAATGCCATTACTTGA+TGG - tig0032655:33917-33936 Msa1449610:intron 40.0%
GAAGGACTTCCATCAAGTAA+TGG + tig0032655:33929-33948 None:intergenic 40.0%
CTCATATCATGACTGGACAT+TGG + tig0032655:34028-34047 None:intergenic 40.0%
ATGTCCAGTCATGATATGAG+AGG - tig0032655:34028-34047 Msa1449610:CDS 40.0%
!! AGAGGAGTCAATAATGGGTT+GGG - tig0032655:34046-34065 Msa1449610:CDS 40.0%
! CATCAGCTGGATTCTTTTTG+TGG - tig0032655:34169-34188 Msa1449610:CDS 40.0%
! ATTTGTTGATGTCGGATGCG+AGG - tig0032655:30688-30707 Msa1449610:CDS 45.0%
TGATGACCAGGCTCTTCTTA+AGG - tig0032655:30887-30906 Msa1449610:CDS 45.0%
GCACCGCCTTTATTATGACT+TGG - tig0032655:30928-30947 Msa1449610:CDS 45.0%
!! AAGGTAGGTAGGGTTGGTTA+TGG - tig0032655:31087-31106 Msa1449610:intron 45.0%
!! ACAGACTTGTGGTCTTGTGT+TGG + tig0032655:31171-31190 None:intergenic 45.0%
TTATGTGTGTTGCGGATCCT+CGG + tig0032655:31249-31268 None:intergenic 45.0%
TTAAGCGACCCCTATTTGCA+GGG + tig0032655:31822-31841 None:intergenic 45.0%
TTTAAGCGACCCCTATTTGC+AGG + tig0032655:31821-31840 None:intergenic 45.0%
TCATTAACTGGGTCAAGGGA+CGG + tig0032655:31413-31432 None:intergenic 45.0%
GCAGTCATTAACTGGGTCAA+GGG + tig0032655:31417-31436 None:intergenic 45.0%
TGACGTGGCAGTCATTAACT+GGG + tig0032655:31725-31744 None:intergenic 45.0%
ATGACGTGGCAGTCATTAAC+TGG + tig0032655:31724-31743 None:intergenic 45.0%
CGTCAATTCCCATGCCATTA+TGG + tig0032655:31456-31475 None:intergenic 45.0%
GGAATTGACGAAAATACCCC+TGG - tig0032655:31466-31485 Msa1449610:CDS 45.0%
TGACGTGGCAGTCATTAACT+TGG - tig0032655:31725-31744 Msa1449610:intron 45.0%
! GGCAGTCATTAACTTGGTCA+AGG - tig0032655:31731-31750 Msa1449610:intron 45.0%
TTTAAGCGACCCCTATTTGC+AGG - tig0032655:31821-31840 Msa1449610:CDS 45.0%
TTAAGCGACCCCTATTTGCA+GGG - tig0032655:31822-31841 Msa1449610:CDS 45.0%
GTCTGTGGTCCAATATCAGA+AGG + tig0032655:32789-32808 None:intergenic 45.0%
GATATTGGACCACAGACATC+TGG - tig0032655:32792-32811 Msa1449610:intron 45.0%
CGACTTCAGCCTACTGTAAA+AGG - tig0032655:32828-32847 Msa1449610:intron 45.0%
GACATGATCATAGTTGCTGC+TGG - tig0032655:33009-33028 Msa1449610:intron 45.0%
CATGATCATAGTTGCTGCTG+GGG - tig0032655:33011-33030 Msa1449610:intron 45.0%
ACAGGTTTCAGTGGCATGTA+GGG + tig0032655:33039-33058 None:intergenic 45.0%
AACAGGTTTCAGTGGCATGT+AGG + tig0032655:33040-33059 None:intergenic 45.0%
GTTGACCTGATTGTGACAAC+AGG + tig0032655:33057-33076 None:intergenic 45.0%
ACAATCAGGTCAACGACAAG+TGG - tig0032655:33063-33082 Msa1449610:intron 45.0%
GCCTAATGACATTGCCTTTC+AGG - tig0032655:33218-33237 Msa1449610:intron 45.0%
CAGAGATCAGAGAAGAGACT+TGG + tig0032655:33342-33361 None:intergenic 45.0%
GCAGCAATATTCCAACTGGA+AGG - tig0032655:33752-33771 Msa1449610:intron 45.0%
GCATCAGCTGATAACTCAGA+AGG + tig0032655:33947-33966 None:intergenic 45.0%
GACTCCTCTCATATCATGAC+TGG + tig0032655:34035-34054 None:intergenic 45.0%
!! GAGAGGAGTCAATAATGGGT+TGG - tig0032655:34045-34064 Msa1449610:CDS 45.0%
!! GGATAGCTTTGATCATCAGC+TGG - tig0032655:34156-34175 Msa1449610:CDS 45.0%
!!! AAACTAAATTTTATTAATTT+AGG + tig0032655:31023-31042 None:intergenic 5.0%
!! TAGTTTAATATATATAATAT+AGG - tig0032655:31280-31299 Msa1449610:intron 5.0%
!!! TGAAAAAAAAAATTTTTTAA+AGG + tig0032655:31505-31524 None:intergenic 5.0%
!! AATTAAAAGTAAAAAAAATT+CGG - tig0032655:31569-31588 Msa1449610:intron 5.0%
!! GTTTGGCCTTAAGAAGAGCC+TGG + tig0032655:30896-30915 None:intergenic 50.0%
ATTACGGTCTCTTGCATCCG+AGG - tig0032655:31229-31248 Msa1449610:intron 50.0%
GGCAGTCATTAACTGGGTCA+AGG + tig0032655:31418-31437 None:intergenic 50.0%
TCTGAAGGACCAGATGTCTG+TGG + tig0032655:32804-32823 None:intergenic 50.0%
! GGCTGAAGTCGAAGTTCTGA+AGG + tig0032655:32819-32838 None:intergenic 50.0%
TCAACGACAAGTGGATCCTC+AGG - tig0032655:33072-33091 Msa1449610:intron 50.0%
CAACGACAAGTGGATCCTCA+GGG - tig0032655:33073-33092 Msa1449610:intron 50.0%
AACGACAAGTGGATCCTCAG+GGG - tig0032655:33074-33093 Msa1449610:intron 50.0%
AGAAGCTTTCTCTTCCCCTG+AGG + tig0032655:33091-33110 None:intergenic 50.0%
AGGGGAAGAGAAAGCTTCTC+AGG - tig0032655:33092-33111 Msa1449610:intron 50.0%
GGGGAAGAGAAAGCTTCTCA+GGG - tig0032655:33093-33112 Msa1449610:intron 50.0%
AGAGAAAGCTTCTCAGGGAG+AGG - tig0032655:33098-33117 Msa1449610:intron 50.0%
AAGCATACGCAGTCAGCACA+TGG - tig0032655:33602-33621 Msa1449610:intron 50.0%
AGCATACGCAGTCAGCACAT+GGG - tig0032655:33603-33622 Msa1449610:intron 50.0%
!! GTCAGCGCATTGGTTGCTAT+TGG + tig0032655:33723-33742 None:intergenic 50.0%
!! GGAGTCAATAATGGGTTGGG+AGG - tig0032655:34049-34068 Msa1449610:CDS 50.0%
AGAAGAACACTGCAGCAGGA+TGG - tig0032655:34098-34117 Msa1449610:CDS 50.0%
GAAGAACAAATTCCCTGCCG+CGG - tig0032655:34131-34150 Msa1449610:CDS 50.0%
AAGAACAAATTCCCTGCCGC+GGG - tig0032655:34132-34151 Msa1449610:CDS 50.0%
TGATCAAAGCTATCCACCCG+CGG + tig0032655:34151-34170 None:intergenic 50.0%
ATGTCGGATGCGAGGAACGA+AGG - tig0032655:30696-30715 Msa1449610:CDS 55.0%
CATTCACGCAGTCAGCGCAT+TGG + tig0032655:33733-33752 None:intergenic 55.0%
GTGCAGAAGAACACTGCAGC+AGG - tig0032655:34094-34113 Msa1449610:CDS 55.0%
GCAGCAGGATGGAACGAGAT+AGG - tig0032655:34109-34128 Msa1449610:CDS 55.0%
AACAAATTCCCTGCCGCGGG+TGG - tig0032655:34135-34154 Msa1449610:CDS 60.0%
AAAGCTATCCACCCGCGGCA+GGG + tig0032655:34146-34165 None:intergenic 60.0%
CAAAGCTATCCACCCGCGGC+AGG + tig0032655:34147-34166 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0032655 gene 30678 34231 30678 ID=Msa1449610;Name=Msa1449610
tig0032655 mRNA 30678 34231 30678 ID=Msa1449610-mRNA-1;Parent=Msa1449610;Name=Msa1449610-mRNA-1;_AED=0.03;_eAED=0.04;_QI=0|0|0|1|1|1|6|0|378
tig0032655 exon 33959 34231 33959 ID=Msa1449610-mRNA-1:exon:17025;Parent=Msa1449610-mRNA-1
tig0032655 exon 32060 32132 32060 ID=Msa1449610-mRNA-1:exon:17024;Parent=Msa1449610-mRNA-1
tig0032655 exon 31740 31929 31740 ID=Msa1449610-mRNA-1:exon:17023;Parent=Msa1449610-mRNA-1
tig0032655 exon 31594 31669 31594 ID=Msa1449610-mRNA-1:exon:17022;Parent=Msa1449610-mRNA-1
tig0032655 exon 31400 31525 31400 ID=Msa1449610-mRNA-1:exon:17021;Parent=Msa1449610-mRNA-1
tig0032655 exon 30678 31076 30678 ID=Msa1449610-mRNA-1:exon:17020;Parent=Msa1449610-mRNA-1
tig0032655 CDS 33959 34231 33959 ID=Msa1449610-mRNA-1:cds;Parent=Msa1449610-mRNA-1
tig0032655 CDS 32060 32132 32060 ID=Msa1449610-mRNA-1:cds;Parent=Msa1449610-mRNA-1
tig0032655 CDS 31740 31929 31740 ID=Msa1449610-mRNA-1:cds;Parent=Msa1449610-mRNA-1
tig0032655 CDS 31594 31669 31594 ID=Msa1449610-mRNA-1:cds;Parent=Msa1449610-mRNA-1
tig0032655 CDS 31400 31525 31400 ID=Msa1449610-mRNA-1:cds;Parent=Msa1449610-mRNA-1
tig0032655 CDS 30678 31076 30678 ID=Msa1449610-mRNA-1:cds;Parent=Msa1449610-mRNA-1
Gene Sequence

>Msa1449610

ATGGAACAATATTTGTTGATGTCGGATGCGAGGAACGAAGGAGAAACAAAGATGAATCGGATTGATTCAAAATTAGATTTTCTGCAGTGTCTAAAGGAAATTGATAAAGGGACAAATACTGTAAAATTTTGTTCTCCACCTTTAACTTCTTCCACCTTTGTTAATAACAATCTCAAAATCAATAATAATATTATTGATTTAGAGAAAGATGATGACCAGGCTCTTCTTAAGGCCAAACTTGATCTTGAATGCACCGCCTTTATTATGACTTGGGGATCTGATCCTTCTGATATTGGACCACAGACATCTGGTCCTTCAGAACTTCGACTTCAGCCTACTGTAAAAGAGTGTGGCCCTTCTGAATTACAAAATAAGGACATGATCATAGTTGCTGCTGGGGCACCCTACATGCCACTGAAACCTGTTGTCACAATCAGGTCAACGACAAGTGGATCCTCAGGGGAAGAGAAAGCTTCTCAGGGAGAGGTTAATATGAATATTGGAGACAATAACCCTGATGCAAAACGAGTTAAGAGGATGCTCCAAAATAGGGAATCAGCTAAACGCTCGAGAGATAGAAAGATAGCTGAAAATACTAAGCTTGAAACACAGGTCTCTACATTAAGAGGTGATAATTCTTCATTGTTGAAGCGTTTATCAGATGTGAGTCAAAAATATGACAATTCTGCTGTTGACAACAGAATATTAAACGCTAATTTTGAAACAATAAAAGCGAAGGTGAATGCGGCAAAAGAGAGATGTAAAAGATTAACTGGATCAATTCCGGCATTTAATGCAATGCCAATGTCTGAGGCAACATCAATGGGAATGCCATTACTTGATGGAAGTCCTTCTGAGTTATCAGCTGATGCATATGTACCTGTGCAAGAAGATCCAAATAATTTCTTTTTTCAACCAACTTTTAATAATCCAATGTCCAGTCATGATATGAGAGGAGTCAATAATGGGTTGGGAGGCATTATTTCTTCAGTTGAAAGTGTGCAGAAGAACACTGCAGCAGGATGGAACGAGATAGGAAGAACAAATTCCCTGCCGCGGGTGGATAGCTTTGATCATCAGCTGGATTCTTTTTGTGGTGATGCAATGCCGAAGAAGACAAGTGATAGAACAATTTGA
Protein sequence

>Msa1449610

MEQYLLMSDARNEGETKMNRIDSKLDFLQCLKEIDKGTNTVKFCSPPLTSSTFVNNNLKINNNIIDLEKDDDQALLKAKLDLECTAFIMTWGSDPSDIGPQTSGPSELRLQPTVKECGPSELQNKDMIIVAAGAPYMPLKPVVTIRSTTSGSSGEEKASQGEVNMNIGDNNPDAKRVKRMLQNRESAKRSRDRKIAENTKLETQVSTLRGDNSSLLKRLSDVSQKYDNSAVDNRILNANFETIKAKVNAAKERCKRLTGSIPAFNAMPMSEATSMGMPLLDGSPSELSADAYVPVQEDPNNFFFQPTFNNPMSSHDMRGVNNGLGGIISSVESVQKNTAAGWNEIGRTNSLPRVDSFDHQLDSFCGDAMPKKTSDRTI*