AlfalfaGEDB | Msa1449660

M. sativa cultivar zhongmu-4 / Msa1449660

KEGG: -
GO: -
PFAM: PF13912.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1449660	A0A072VNE8	88.889	270	26	3	1	266	1	270	4.85e-150	430

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TFs/TRs:

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Gene ID	Type	Classification
Msa1449660	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1449660	MtrunA17_Chr1g0193731	88.889	270	26	3	1	266	1	270	9.33e-154	430
Msa1449660	MtrunA17_Chr7g0214511	47.249	309	81	14	8	267	12	287	3.18e-56	182
Msa1449660	MtrunA17_Chr1g0180581	46.693	257	61	10	15	242	10	219	7.87e-51	166
Msa1449660	MtrunA17_Chr1g0169521	36.538	260	105	8	41	267	84	316	1.29e-31	119
Msa1449660	MtrunA17_Chr1g0169561	35.385	260	110	8	40	267	83	316	2.44e-29	112
Msa1449660	MtrunA17_Chr1g0174001	38.068	176	75	7	103	267	43	195	3.49e-19	82.8
Msa1449660	MtrunA17_Chr1g0180571	62.963	81	26	2	185	261	43	123	2.06e-18	79.3
Msa1449660	MtrunA17_Chr1g0172861	33.953	215	104	11	73	267	82	278	9.30e-16	75.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 40 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTAAGATGAGGCACTAAT+TGG	0.271486	tig0032664:-22738	None:intergenic
AATGAAGGCGAAACTGAATT+AGG	0.231002	tig0032664:+22653	Msa1449660:CDS
AATGAAGGTGAAACTGAATT+AGG	0.203359	tig0032664:+22509	Msa1449660:CDS
AATGAAGGTGAAACTGAATT+AGG	0.203359	tig0032664:+22581	Msa1449660:CDS
AATGGACATATGAGATGTCA+CGG	0.619984	tig0032664:+22206	Msa1449660:CDS
ACCCTGAGAAGAAGATCATA+TGG	0.501896	tig0032664:+22081	Msa1449660:CDS
ACTCAACGAAAGAGAAAGAA+GGG	0.591599	tig0032664:-22028	None:intergenic
ACTCCTAACAAAGCATTGAA+TGG	0.427181	tig0032664:+22188	Msa1449660:CDS
ATATATCTTCTTCTCCTGCA+CGG	0.556539	tig0032664:-22275	None:intergenic
ATGAAGGCGAAACTGAATTA+GGG	0.437450	tig0032664:+22654	Msa1449660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGAAGAAGAAGATTAATGA+AGG	+	tig0032664:22494-22513	Msa1449660:CDS	30.0%
AATGAAGGTGAAACTGAATT+AGG	+	tig0032664:22509-22528	Msa1449660:CDS	30.0%
ATGAAGGTGAAACTGAATTA+GGG	+	tig0032664:22510-22529	Msa1449660:CDS	30.0%
AATGAAGGTGAAACTGAATT+AGG	+	tig0032664:22509-22528	Msa1449660:CDS	30.0%
ATGAAGGTGAAACTGAATTA+GGG	+	tig0032664:22510-22529	Msa1449660:CDS	30.0%
GTCAAAAGTTGTGAAGAATT+TGG	+	tig0032664:22604-22623	Msa1449660:CDS	30.0%
GAGAAGAAGAAGATTAATGA+AGG	+	tig0032664:22494-22513	Msa1449660:CDS	30.0%
AATGAAGGTGAAACTGAATT+AGG	+	tig0032664:22509-22528	Msa1449660:CDS	30.0%
ATGAAGGTGAAACTGAATTA+GGG	+	tig0032664:22510-22529	Msa1449660:CDS	30.0%
AATGAAGGTGAAACTGAATT+AGG	+	tig0032664:22509-22528	Msa1449660:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0032664	gene	21942	22745	21942	ID=Msa1449660;Name=Msa1449660
tig0032664	mRNA	21942	22745	21942	ID=Msa1449660-mRNA-1;Parent=Msa1449660;Name=Msa1449660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|267
tig0032664	exon	21942	22745	21942	ID=Msa1449660-mRNA-1:exon:17034;Parent=Msa1449660-mRNA-1
tig0032664	CDS	21942	22745	21942	ID=Msa1449660-mRNA-1:cds;Parent=Msa1449660-mRNA-1

Gene Sequence

>Msa1449660

ATGTATTCTTCTTCATCTTCTTCTCATGATCATTCTTTCCATCAAAAACCAAAAACCAATAATCAGTCTCTTCAAAAAACCAGATACCCTTCTTTCTCTTTCGTTGAGTCCTCAACAACTAATGATGCTGGAAAAGAGAACCCTGAGAAGAAGATCATATGGCGTTTTTCTTATTCTGTTGATTATAATCATAATGATGTAGTAAAACAGAAGAAACACTCGTGTGATGTTTGCAACAAGGTTTTTACTCCTAACAAAGCATTGAATGGACATATGAGATGTCACGGTCAAAACGGTCCAACAGTTCCAGCACCATCTGCCGAGAAACAGCAGCCGTGCAGGAGAAGAAGATATATGAATATTGACGACGACGACGATGTTATTGAAACAGTTGCAGAAATTCTATTGCGTCTATCTCGTGTGGGTTATAAGGATGTGAATCACCGTGGTGCTAAAAGGCAAAAGCTTTCTTGCAATATGATCGATGAGAAGTCGACGAAGAAGAAAAAGGAACAAGGTGTTCTACTAATAAGTGGTTGTCATGATATTGAAGAGAAGAAGAAGATTAATGAAGGTGAAACTGAATTAGGGTCAAAAGTTGTGAAGAATGTTGATTTGAACGAGCTTCCAAGTGATGATAATGAAGGTGAAACTGAATTAGGGTCAAAAGTTGTGAAGAATTTGGATTTGAACGAGCTTCCAAGTGATGATAATGAAGGCGAAACTGAATTAGGGTCAAAAGTTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTGATGAGTTTGAAGATGAAACCAATTAG

Protein sequence

>Msa1449660

MYSSSSSSHDHSFHQKPKTNNQSLQKTRYPSFSFVESSTTNDAGKENPEKKIIWRFSYSVDYNHNDVVKQKKHSCDVCNKVFTPNKALNGHMRCHGQNGPTVPAPSAEKQQPCRRRRYMNIDDDDDVIETVAEILLRLSRVGYKDVNHRGAKRQKLSCNMIDEKSTKKKKEQGVLLISGCHDIEEKKKINEGETELGSKVVKNVDLNELPSDDNEGETELGSKVVKNLDLNELPSDDNEGETELGSKVVKNFDLNELPSDEFEDETN*