Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449660 | A0A072VNE8 | 88.889 | 270 | 26 | 3 | 1 | 266 | 1 | 270 | 4.85e-150 | 430 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1449660 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449660 | MtrunA17_Chr1g0193731 | 88.889 | 270 | 26 | 3 | 1 | 266 | 1 | 270 | 9.33e-154 | 430 |
| Msa1449660 | MtrunA17_Chr7g0214511 | 47.249 | 309 | 81 | 14 | 8 | 267 | 12 | 287 | 3.18e-56 | 182 |
| Msa1449660 | MtrunA17_Chr1g0180581 | 46.693 | 257 | 61 | 10 | 15 | 242 | 10 | 219 | 7.87e-51 | 166 |
| Msa1449660 | MtrunA17_Chr1g0169521 | 36.538 | 260 | 105 | 8 | 41 | 267 | 84 | 316 | 1.29e-31 | 119 |
| Msa1449660 | MtrunA17_Chr1g0169561 | 35.385 | 260 | 110 | 8 | 40 | 267 | 83 | 316 | 2.44e-29 | 112 |
| Msa1449660 | MtrunA17_Chr1g0174001 | 38.068 | 176 | 75 | 7 | 103 | 267 | 43 | 195 | 3.49e-19 | 82.8 |
| Msa1449660 | MtrunA17_Chr1g0180571 | 62.963 | 81 | 26 | 2 | 185 | 261 | 43 | 123 | 2.06e-18 | 79.3 |
| Msa1449660 | MtrunA17_Chr1g0172861 | 33.953 | 215 | 104 | 11 | 73 | 267 | 82 | 278 | 9.30e-16 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAAGGTGAAACTGAATT+AGG | 0.203359 | tig0032664:+22509 | Msa1449660:CDS |
| AATGAAGGTGAAACTGAATT+AGG | 0.203359 | tig0032664:+22581 | Msa1449660:CDS |
| GTCAAAAGTTGTGAAGAATT+TGG | 0.225875 | tig0032664:+22604 | Msa1449660:CDS |
| AATGAAGGCGAAACTGAATT+AGG | 0.231002 | tig0032664:+22653 | Msa1449660:CDS |
| AACTAAGATGAGGCACTAAT+TGG | 0.271486 | tig0032664:-22738 | None:intergenic |
| TGTCCATTCAATGCTTTGTT+AGG | 0.311576 | tig0032664:-22191 | None:intergenic |
| TGAATCACCGTGGTGCTAAA+AGG | 0.329449 | tig0032664:+22378 | Msa1449660:CDS |
| CGCCATATGATCTTCTTCTC+AGG | 0.345803 | tig0032664:-22083 | None:intergenic |
| TCTATCTCGTGTGGGTTATA+AGG | 0.362072 | tig0032664:+22352 | Msa1449660:CDS |
| CCTGCACGGCTGCTGTTTCT+CGG | 0.365481 | tig0032664:-22261 | None:intergenic |
| GAAAGAGAAAGAAGGGTATC+TGG | 0.402663 | tig0032664:-22021 | None:intergenic |
| ATGAAGGTGAAACTGAATTA+GGG | 0.420619 | tig0032664:+22510 | Msa1449660:CDS |
| ACTCCTAACAAAGCATTGAA+TGG | 0.427181 | tig0032664:+22188 | Msa1449660:CDS |
| GAAGTCGACGAAGAAGAAAA+AGG | 0.429841 | tig0032664:+22430 | Msa1449660:CDS |
| ATGAAGGTGAAACTGAATTA+GGG | 0.430586 | tig0032664:+22582 | Msa1449660:CDS |
| ATGAAGGCGAAACTGAATTA+GGG | 0.437450 | tig0032664:+22654 | Msa1449660:CDS |
| GGCTGCTGTTTCTCGGCAGA+TGG | 0.440268 | tig0032664:-22254 | None:intergenic |
| CCGAGAAACAGCAGCCGTGC+AGG | 0.448055 | tig0032664:+22261 | Msa1449660:CDS |
| TGTTTCTCGGCAGATGGTGC+TGG | 0.465904 | tig0032664:-22248 | None:intergenic |
| TCCTCAACAACTAATGATGC+TGG | 0.472142 | tig0032664:+22050 | Msa1449660:CDS |
| TCCAGCATCATTAGTTGTTG+AGG | 0.475624 | tig0032664:-22051 | None:intergenic |
| GAGAAGAAGAAGATTAATGA+AGG | 0.491417 | tig0032664:+22494 | Msa1449660:CDS |
| TCTATTGCGTCTATCTCGTG+TGG | 0.500480 | tig0032664:+22343 | Msa1449660:CDS |
| ACCCTGAGAAGAAGATCATA+TGG | 0.501896 | tig0032664:+22081 | Msa1449660:CDS |
| ATGAGATGTCACGGTCAAAA+CGG | 0.520374 | tig0032664:+22215 | Msa1449660:CDS |
| CTCGTGTGATGTTTGCAACA+AGG | 0.520809 | tig0032664:+22160 | Msa1449660:CDS |
| TCATCTTCAAACTCATCACT+TGG | 0.524025 | tig0032664:-22713 | None:intergenic |
| GCAGATGGTGCTGGAACTGT+TGG | 0.533618 | tig0032664:-22239 | None:intergenic |
| ATATATCTTCTTCTCCTGCA+CGG | 0.556539 | tig0032664:-22275 | None:intergenic |
| GCCATATGATCTTCTTCTCA+GGG | 0.557187 | tig0032664:-22082 | None:intergenic |
| TCGCCTTCATTATCATCACT+TGG | 0.560001 | tig0032664:-22641 | None:intergenic |
| GACTCAACGAAAGAGAAAGA+AGG | 0.560928 | tig0032664:-22029 | None:intergenic |
| CAAGGTGTTCTACTAATAAG+TGG | 0.561004 | tig0032664:+22455 | Msa1449660:CDS |
| TCACCTTCATTATCATCACT+TGG | 0.567032 | tig0032664:-22569 | None:intergenic |
| CTATTGCGTCTATCTCGTGT+GGG | 0.577229 | tig0032664:+22344 | Msa1449660:CDS |
| CTTCCAAGTGATGATAATGA+AGG | 0.580430 | tig0032664:+22638 | Msa1449660:CDS |
| CTTCCAAGTGATGATAATGA+AGG | 0.583048 | tig0032664:+22566 | Msa1449660:CDS |
| ACTCAACGAAAGAGAAAGAA+GGG | 0.591599 | tig0032664:-22028 | None:intergenic |
| AATGGACATATGAGATGTCA+CGG | 0.619984 | tig0032664:+22206 | Msa1449660:CDS |
| TATAAGGATGTGAATCACCG+TGG | 0.764003 | tig0032664:+22368 | Msa1449660:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTGGTTTTTGGTTTTTGA+TGG | - | tig0032664:21983-22002 | None:intergenic | 25.0% |
| !!! | TTTTTGAAGAGACTGATTAT+TGG | - | tig0032664:22000-22019 | None:intergenic | 25.0% |
| !!! | TATTGGTTTTTGGTTTTTGA+TGG | - | tig0032664:21983-22002 | None:intergenic | 25.0% |
| !!! | TTTTTGAAGAGACTGATTAT+TGG | - | tig0032664:22000-22019 | None:intergenic | 25.0% |
| !!! | AGAGACTGATTATTGGTTTT+TGG | - | tig0032664:21993-22012 | None:intergenic | 30.0% |
| GAGAAGAAGAAGATTAATGA+AGG | + | tig0032664:22494-22513 | Msa1449660:CDS | 30.0% | |
| AATGAAGGTGAAACTGAATT+AGG | + | tig0032664:22509-22528 | Msa1449660:CDS | 30.0% | |
| ATGAAGGTGAAACTGAATTA+GGG | + | tig0032664:22510-22529 | Msa1449660:CDS | 30.0% | |
| AATGAAGGTGAAACTGAATT+AGG | + | tig0032664:22509-22528 | Msa1449660:CDS | 30.0% | |
| ATGAAGGTGAAACTGAATTA+GGG | + | tig0032664:22510-22529 | Msa1449660:CDS | 30.0% | |
| GTCAAAAGTTGTGAAGAATT+TGG | + | tig0032664:22604-22623 | Msa1449660:CDS | 30.0% | |
| !!! | AGAGACTGATTATTGGTTTT+TGG | - | tig0032664:21993-22012 | None:intergenic | 30.0% |
| GAGAAGAAGAAGATTAATGA+AGG | + | tig0032664:22494-22513 | Msa1449660:CDS | 30.0% | |
| AATGAAGGTGAAACTGAATT+AGG | + | tig0032664:22509-22528 | Msa1449660:CDS | 30.0% | |
| ATGAAGGTGAAACTGAATTA+GGG | + | tig0032664:22510-22529 | Msa1449660:CDS | 30.0% | |
| AATGAAGGTGAAACTGAATT+AGG | + | tig0032664:22509-22528 | Msa1449660:CDS | 30.0% | |
| ATGAAGGTGAAACTGAATTA+GGG | + | tig0032664:22510-22529 | Msa1449660:CDS | 30.0% | |
| GTCAAAAGTTGTGAAGAATT+TGG | + | tig0032664:22604-22623 | Msa1449660:CDS | 30.0% | |
| ACTCAACGAAAGAGAAAGAA+GGG | - | tig0032664:22031-22050 | None:intergenic | 35.0% | |
| !! | ACTCCTAACAAAGCATTGAA+TGG | + | tig0032664:22188-22207 | Msa1449660:CDS | 35.0% |
| ! | TGTCCATTCAATGCTTTGTT+AGG | - | tig0032664:22194-22213 | None:intergenic | 35.0% |
| AATGGACATATGAGATGTCA+CGG | + | tig0032664:22206-22225 | Msa1449660:CDS | 35.0% | |
| ATATATCTTCTTCTCCTGCA+CGG | - | tig0032664:22278-22297 | None:intergenic | 35.0% | |
| ACGAAGAAGAAAAAGGAACA+AGG | + | tig0032664:22437-22456 | Msa1449660:CDS | 35.0% | |
| ! | CAAGGTGTTCTACTAATAAG+TGG | + | tig0032664:22455-22474 | Msa1449660:CDS | 35.0% |
| ! | CTTCCAAGTGATGATAATGA+AGG | + | tig0032664:22566-22585 | Msa1449660:CDS | 35.0% |
| TCACCTTCATTATCATCACT+TGG | - | tig0032664:22572-22591 | None:intergenic | 35.0% | |
| ! | CTTCCAAGTGATGATAATGA+AGG | + | tig0032664:22566-22585 | Msa1449660:CDS | 35.0% |
| AATGAAGGCGAAACTGAATT+AGG | + | tig0032664:22653-22672 | Msa1449660:CDS | 35.0% | |
| ATGAAGGCGAAACTGAATTA+GGG | + | tig0032664:22654-22673 | Msa1449660:CDS | 35.0% | |
| TCATCTTCAAACTCATCACT+TGG | - | tig0032664:22716-22735 | None:intergenic | 35.0% | |
| ACTCAACGAAAGAGAAAGAA+GGG | - | tig0032664:22031-22050 | None:intergenic | 35.0% | |
| !! | ACTCCTAACAAAGCATTGAA+TGG | + | tig0032664:22188-22207 | Msa1449660:CDS | 35.0% |
| ! | TGTCCATTCAATGCTTTGTT+AGG | - | tig0032664:22194-22213 | None:intergenic | 35.0% |
| AATGGACATATGAGATGTCA+CGG | + | tig0032664:22206-22225 | Msa1449660:CDS | 35.0% | |
| ATATATCTTCTTCTCCTGCA+CGG | - | tig0032664:22278-22297 | None:intergenic | 35.0% | |
| ACGAAGAAGAAAAAGGAACA+AGG | + | tig0032664:22437-22456 | Msa1449660:CDS | 35.0% | |
| ! | CAAGGTGTTCTACTAATAAG+TGG | + | tig0032664:22455-22474 | Msa1449660:CDS | 35.0% |
| ! | CTTCCAAGTGATGATAATGA+AGG | + | tig0032664:22566-22585 | Msa1449660:CDS | 35.0% |
| TCACCTTCATTATCATCACT+TGG | - | tig0032664:22572-22591 | None:intergenic | 35.0% | |
| ! | CTTCCAAGTGATGATAATGA+AGG | + | tig0032664:22566-22585 | Msa1449660:CDS | 35.0% |
| AATGAAGGCGAAACTGAATT+AGG | + | tig0032664:22653-22672 | Msa1449660:CDS | 35.0% | |
| ATGAAGGCGAAACTGAATTA+GGG | + | tig0032664:22654-22673 | Msa1449660:CDS | 35.0% | |
| TCATCTTCAAACTCATCACT+TGG | - | tig0032664:22716-22735 | None:intergenic | 35.0% | |
| !! | GAAAGAGAAAGAAGGGTATC+TGG | - | tig0032664:22024-22043 | None:intergenic | 40.0% |
| GACTCAACGAAAGAGAAAGA+AGG | - | tig0032664:22032-22051 | None:intergenic | 40.0% | |
| TCCTCAACAACTAATGATGC+TGG | + | tig0032664:22050-22069 | Msa1449660:CDS | 40.0% | |
| TCCAGCATCATTAGTTGTTG+AGG | - | tig0032664:22054-22073 | None:intergenic | 40.0% | |
| ACCCTGAGAAGAAGATCATA+TGG | + | tig0032664:22081-22100 | Msa1449660:CDS | 40.0% | |
| GCCATATGATCTTCTTCTCA+GGG | - | tig0032664:22085-22104 | None:intergenic | 40.0% | |
| ATGAGATGTCACGGTCAAAA+CGG | + | tig0032664:22215-22234 | Msa1449660:CDS | 40.0% | |
| ! | TCTATCTCGTGTGGGTTATA+AGG | + | tig0032664:22352-22371 | Msa1449660:CDS | 40.0% |
| TATAAGGATGTGAATCACCG+TGG | + | tig0032664:22368-22387 | Msa1449660:CDS | 40.0% | |
| GAAGTCGACGAAGAAGAAAA+AGG | + | tig0032664:22430-22449 | Msa1449660:CDS | 40.0% | |
| TCGCCTTCATTATCATCACT+TGG | - | tig0032664:22644-22663 | None:intergenic | 40.0% | |
| !! | GAAAGAGAAAGAAGGGTATC+TGG | - | tig0032664:22024-22043 | None:intergenic | 40.0% |
| GACTCAACGAAAGAGAAAGA+AGG | - | tig0032664:22032-22051 | None:intergenic | 40.0% | |
| TCCTCAACAACTAATGATGC+TGG | + | tig0032664:22050-22069 | Msa1449660:CDS | 40.0% | |
| TCCAGCATCATTAGTTGTTG+AGG | - | tig0032664:22054-22073 | None:intergenic | 40.0% | |
| ACCCTGAGAAGAAGATCATA+TGG | + | tig0032664:22081-22100 | Msa1449660:CDS | 40.0% | |
| GCCATATGATCTTCTTCTCA+GGG | - | tig0032664:22085-22104 | None:intergenic | 40.0% | |
| ATGAGATGTCACGGTCAAAA+CGG | + | tig0032664:22215-22234 | Msa1449660:CDS | 40.0% | |
| ! | TCTATCTCGTGTGGGTTATA+AGG | + | tig0032664:22352-22371 | Msa1449660:CDS | 40.0% |
| TATAAGGATGTGAATCACCG+TGG | + | tig0032664:22368-22387 | Msa1449660:CDS | 40.0% | |
| GAAGTCGACGAAGAAGAAAA+AGG | + | tig0032664:22430-22449 | Msa1449660:CDS | 40.0% | |
| TCGCCTTCATTATCATCACT+TGG | - | tig0032664:22644-22663 | None:intergenic | 40.0% | |
| CGCCATATGATCTTCTTCTC+AGG | - | tig0032664:22086-22105 | None:intergenic | 45.0% | |
| CTCGTGTGATGTTTGCAACA+AGG | + | tig0032664:22160-22179 | Msa1449660:CDS | 45.0% | |
| TCTATTGCGTCTATCTCGTG+TGG | + | tig0032664:22343-22362 | Msa1449660:CDS | 45.0% | |
| CTATTGCGTCTATCTCGTGT+GGG | + | tig0032664:22344-22363 | Msa1449660:CDS | 45.0% | |
| ! | TGAATCACCGTGGTGCTAAA+AGG | + | tig0032664:22378-22397 | Msa1449660:CDS | 45.0% |
| ! | GCTTTTGCCTTTTAGCACCA+CGG | - | tig0032664:22388-22407 | None:intergenic | 45.0% |
| CGCCATATGATCTTCTTCTC+AGG | - | tig0032664:22086-22105 | None:intergenic | 45.0% | |
| CTCGTGTGATGTTTGCAACA+AGG | + | tig0032664:22160-22179 | Msa1449660:CDS | 45.0% | |
| TCTATTGCGTCTATCTCGTG+TGG | + | tig0032664:22343-22362 | Msa1449660:CDS | 45.0% | |
| CTATTGCGTCTATCTCGTGT+GGG | + | tig0032664:22344-22363 | Msa1449660:CDS | 45.0% | |
| ! | TGAATCACCGTGGTGCTAAA+AGG | + | tig0032664:22378-22397 | Msa1449660:CDS | 45.0% |
| ! | GCTTTTGCCTTTTAGCACCA+CGG | - | tig0032664:22388-22407 | None:intergenic | 45.0% |
| ! | GCAGATGGTGCTGGAACTGT+TGG | - | tig0032664:22242-22261 | None:intergenic | 55.0% |
| TGTTTCTCGGCAGATGGTGC+TGG | - | tig0032664:22251-22270 | None:intergenic | 55.0% | |
| ! | GCAGATGGTGCTGGAACTGT+TGG | - | tig0032664:22242-22261 | None:intergenic | 55.0% |
| TGTTTCTCGGCAGATGGTGC+TGG | - | tig0032664:22251-22270 | None:intergenic | 55.0% | |
| !! | GGCTGCTGTTTCTCGGCAGA+TGG | - | tig0032664:22257-22276 | None:intergenic | 60.0% |
| !! | CCTGCACGGCTGCTGTTTCT+CGG | - | tig0032664:22264-22283 | None:intergenic | 60.0% |
| !! | GGCTGCTGTTTCTCGGCAGA+TGG | - | tig0032664:22257-22276 | None:intergenic | 60.0% |
| !! | CCTGCACGGCTGCTGTTTCT+CGG | - | tig0032664:22264-22283 | None:intergenic | 60.0% |
| CCGAGAAACAGCAGCCGTGC+AGG | + | tig0032664:22261-22280 | Msa1449660:CDS | 65.0% | |
| CCGAGAAACAGCAGCCGTGC+AGG | + | tig0032664:22261-22280 | Msa1449660:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0032664 | gene | 21942 | 22745 | 21942 | ID=Msa1449660;Name=Msa1449660 |
| tig0032664 | mRNA | 21942 | 22745 | 21942 | ID=Msa1449660-mRNA-1;Parent=Msa1449660;Name=Msa1449660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|267 |
| tig0032664 | exon | 21942 | 22745 | 21942 | ID=Msa1449660-mRNA-1:exon:17034;Parent=Msa1449660-mRNA-1 |
| tig0032664 | CDS | 21942 | 22745 | 21942 | ID=Msa1449660-mRNA-1:cds;Parent=Msa1449660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
