AlfalfaGEDB | Msa1450650

M. sativa cultivar zhongmu-4 / Msa1450650

KEGG: K11644
GO: -
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450650	sp\|O48686\|SNL3_ARATH	52.778	72	32	1	8	77	13	84	3.27e-13	75.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450650	A0A2Z6N6C3	58.864	440	146	11	10	436	23	440	5.18e-161	471

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TFs/TRs:

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Gene ID	Type	Classification
Msa1450650	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450650	MtrunA17_Chr7g0252101	58.575	449	155	14	1	436	2	432	3.83e-160	459
Msa1450650	MtrunA17_Chr3g0093911	64.820	361	109	10	83	436	1	350	5.22e-144	414
Msa1450650	MtrunA17_Chr1g0207351	63.812	362	108	11	87	436	16	366	9.26e-142	409
Msa1450650	MtrunA17_Chr4g0075971	54.214	439	180	10	9	436	174	602	2.81e-137	406
Msa1450650	MtrunA17_Chr3g0077801	54.039	359	147	7	83	433	1	349	4.79e-117	346
Msa1450650	MtrunA17_Chr6g0488101	46.137	453	200	12	15	436	16	455	5.75e-116	347
Msa1450650	MtrunA17_Chr6g0488271	46.729	428	191	11	33	436	1	415	3.96e-112	335
Msa1450650	MtrunA17_Chr3g0077901	51.515	363	162	6	79	435	17	371	3.23e-111	332
Msa1450650	MtrunA17_Chr1g0209051	60.727	275	96	5	166	435	3	270	8.40e-104	309
Msa1450650	MtrunA17_Chr3g0077851	51.020	343	154	6	99	435	1	335	1.31e-103	311

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450650	AT1G24190.2	52.778	72	32	1	8	77	13	84	3.32e-14	75.5
Msa1450650	AT1G24190.1	52.778	72	32	1	8	77	13	84	3.32e-14	75.5
Msa1450650	AT1G24190.3	52.778	72	32	1	8	77	13	84	3.48e-14	75.5
Msa1450650	AT1G70060.2	54.286	70	30	1	9	76	14	83	1.62e-12	70.5
Msa1450650	AT1G70060.1	54.286	70	30	1	9	76	14	83	1.62e-12	70.5
Msa1450650	AT3G01320.2	38.017	121	56	4	8	126	56	159	4.72e-12	68.9
Msa1450650	AT3G01320.1	38.017	121	56	4	8	126	56	159	5.21e-12	68.6
Msa1450650	AT1G59890.4	47.436	78	39	1	9	84	45	122	1.38e-11	67.4
Msa1450650	AT1G59890.1	47.436	78	39	1	9	84	45	122	1.46e-11	67.4
Msa1450650	AT1G59890.3	47.436	78	39	1	9	84	45	122	1.48e-11	67.4

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Find 90 sgRNAs with CRISPR-Local

Find 332 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACCAATCAAACGTGT+AGG	0.635770	tig0033035:-28817	None:intergenic
AAAACTCCTAAAAGATGTCA+AGG	0.541090	tig0033035:+23560	Msa1450650:CDS
AAAATGTAGTGTGTATTCCG+GGG	0.780507	tig0033035:+28743	Msa1450650:CDS
AAAGGTTGAGGGTATTTCAT+TGG	0.363083	tig0033035:-28475	None:intergenic
AACCATGAGTTATGTCAAAC+TGG	0.399495	tig0033035:+28599	Msa1450650:CDS
AAGCTGGGTTTCAAGATAAG+AGG	0.471098	tig0033035:+23517	Msa1450650:CDS
AAGGGATGTGTACAGGATAA+GGG	0.471486	tig0033035:+28554	Msa1450650:CDS
AAGGTTGAGGGTATTTCATT+GGG	0.422234	tig0033035:-28474	None:intergenic
AATACCCTCAACCTTTAGGT+TGG	0.533454	tig0033035:+28482	Msa1450650:CDS
AATATATGTTGTCACTTTCG+AGG	0.471894	tig0033035:+28389	Msa1450650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTCCTAAAAGATGTCA+AGG	+	tig0033035:23560-23579	Msa1450650:CDS	30.0%
TATGTTATGGTTGTTTGTGA+TGG	+	tig0033035:23971-23990	Msa1450650:intron	30.0%
TAAGACCTTTATCACATTGT+AGG	+	tig0033035:24144-24163	Msa1450650:intron	30.0%
AGTAGAGTGTAATCATTGAA+GGG	-	tig0033035:24222-24241	None:intergenic	30.0%
TAGTAGAGTGTAATCATTGA+AGG	-	tig0033035:24223-24242	None:intergenic	30.0%
TAACAACCATATTTACTAGC+TGG	-	tig0033035:24279-24298	None:intergenic	30.0%
TCCCATTAGTAATTAACTGA+TGG	+	tig0033035:24403-24422	Msa1450650:intron	30.0%
CTTAGCTTAAAACATTTCGA+TGG	-	tig0033035:24813-24832	None:intergenic	30.0%
TTGAAAGCCCATCATAAATA+AGG	+	tig0033035:25279-25298	Msa1450650:intron	30.0%
CTGTACAACAACTAATGTAT+AGG	-	tig0033035:25365-25384	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0033035	gene	23284	28881	23284	ID=Msa1450650;Name=Msa1450650
tig0033035	mRNA	23284	28881	23284	ID=Msa1450650-mRNA-1;Parent=Msa1450650;Name=Msa1450650-mRNA-1;_AED=0.25;_eAED=0.25;_QI=88\|0.5\|0.66\|1\|0.5\|0.66\|3\|0\|436
tig0033035	exon	23284	23371	23284	ID=Msa1450650-mRNA-1:exon:17576;Parent=Msa1450650-mRNA-1
tig0033035	exon	23471	23708	23471	ID=Msa1450650-mRNA-1:exon:17577;Parent=Msa1450650-mRNA-1
tig0033035	exon	27809	28881	27809	ID=Msa1450650-mRNA-1:exon:17578;Parent=Msa1450650-mRNA-1
tig0033035	five_prime_UTR	23284	23371	23284	ID=Msa1450650-mRNA-1:five_prime_utr;Parent=Msa1450650-mRNA-1
tig0033035	CDS	23471	23708	23471	ID=Msa1450650-mRNA-1:cds;Parent=Msa1450650-mRNA-1
tig0033035	CDS	27809	28881	27809	ID=Msa1450650-mRNA-1:cds;Parent=Msa1450650-mRNA-1

Gene Sequence

>Msa1450650

ATGGAGATCCCAAAAAACCGCGATGATGCGTTGGCAGCGATTGTACAAGCTGGGTTTCAAGATAAGAGGGGAGATTACAATGAGCTTTTAAAACTCCTAAAAGATGTCAAGGATCAAAGAATTTACACAAGAGATGCCGCGGCAAGAGTCAAGCAGCTGCTTAAAGGGCATACTGATTTAATTTTGGGATTCAACACCTTCCTGCCAAAGGAACATCAAATCAAACTTGAGACCACTGGGAAAAAGGTGAAGAATGTACAAGAGAACCGTGAACTTCCCTGTGATGTGCTTGATATCATTTACCAAACATTAGATTTTGATGACTTTTTTCAGTTTGCCGGTGTGTGCAAGAATTGGAGGGCTTTTCATAAAATTCACCGGAGAAATTTTTTGGCATCGCAAGAACCATTAATTCTTCAAGTTTCTTATGTTATTCGATCACTTTCCTTTATCAGCATACCCGACCAAAAAGTTAATTATCGCTTAAACATAGATGAGTGCTTCTGGAATTATACCTATGTTACGTCTTCTAACGGATATTTTATCTCGGTTGGGAGAAATAATTCAGTTTTGCTACTCAATCCATTTACAAGAATAAAGAAGGTAATCAATACATGTCCCTCTGAAATCAAGCCTTATATGGATCTTAACCGTGCTTGGCTTGCTTTTGACAAATGCTCTGAGGAATTTGTCTTGATGGTTTTGTGCTATTTTCCTGAGATCTTGCATGTCTATCAATCTCGAAATTGTGGTTGGGTTACTTATTCGACAATGGGAAATAAAGTAAGAGTTGTTGACGCTGTGGTTTTGCATAATATAATATATGTTGTCACTTTCGAGGGCAACATAGGGGTAATTAGCTTGAATTCATCAAAAATTAAATTTCTAACACTAAAGAATACTCCCAATGAAATACCCTCAACCTTTAGGTTGGTTAATTGTAATGAACAACTTGTAGTGCTTGATTTTAGGTACAATAAAATAAGGGATGTGTACAGGATAAGGGATGTGTACAAGATAGACTTCTCAACCATGAGTTATGTCAAACTGGAAACTTTGGGCGACATTGCGTTATTTCATGCTTCAGAAAGAAATTCTTATGCATTGAGCAACCCGAATAAGTGGGGATATGAGAGCAACTTTGTGTATGCATTGAACGACATTAAATCTGCAAAATGTAGTGTGTATTCCGGGGATGATAAAAAATTGAAAAAATGCATTATGCTTCCAGCTCATGGAGCATATTCCTACACGTTTGATTGGTGTTTTAGACATCAACAATATGAGGTAGATTATTCCCTAGTTGAGTGA

Protein sequence

>Msa1450650

MEIPKNRDDALAAIVQAGFQDKRGDYNELLKLLKDVKDQRIYTRDAAARVKQLLKGHTDLILGFNTFLPKEHQIKLETTGKKVKNVQENRELPCDVLDIIYQTLDFDDFFQFAGVCKNWRAFHKIHRRNFLASQEPLILQVSYVIRSLSFISIPDQKVNYRLNIDECFWNYTYVTSSNGYFISVGRNNSVLLLNPFTRIKKVINTCPSEIKPYMDLNRAWLAFDKCSEEFVLMVLCYFPEILHVYQSRNCGWVTYSTMGNKVRVVDAVVLHNIIYVVTFEGNIGVISLNSSKIKFLTLKNTPNEIPSTFRLVNCNEQLVVLDFRYNKIRDVYRIRDVYKIDFSTMSYVKLETLGDIALFHASERNSYALSNPNKWGYESNFVYALNDIKSAKCSVYSGDDKKLKKCIMLPAHGAYSYTFDWCFRHQQYEVDYSLVE*