AlfalfaGEDB | Msa1454050

M. sativa cultivar zhongmu-4 / Msa1454050

KEGG: K00924|K08959|K08960
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008360 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009639 GO:0009640 GO:0009785 GO:0009791 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0030522 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051716 GO:0065007 GO:0065008 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0098657 GO:0104004 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454050	sp\|Q93Z18\|CKL3_ARATH	70.883	419	107	4	1	410	1	413	0.0	611

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454050	G7KPU7	99.281	417	3	0	1	417	1	417	0.0	864

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454050	MtrunA17_Chr6g0486611	99.281	417	3	0	1	417	1	417	0.0	864
Msa1454050	MtrunA17_Chr8g0390211	80.336	417	79	3	1	416	1	415	0.0	695
Msa1454050	MtrunA17_Chr3g0140071	64.532	406	134	4	1	398	1	404	0.0	524
Msa1454050	MtrunA17_Chr4g0071031	63.636	418	143	4	1	411	1	416	0.0	520
Msa1454050	MtrunA17_Chr2g0329981	63.246	419	138	7	1	411	1	411	0.0	520
Msa1454050	MtrunA17_Chr1g0166821	75.625	320	75	2	1	317	2	321	0.0	518
Msa1454050	MtrunA17_Chr1g0183631	76.299	308	73	0	1	308	1	308	1.14e-178	506
Msa1454050	MtrunA17_Chr8g0342821	77.926	299	65	1	1	299	1	298	1.77e-178	506
Msa1454050	MtrunA17_Chr1g0146151	60.526	418	149	5	1	411	1	409	6.53e-177	500
Msa1454050	MtrunA17_Chr1g0187571	72.586	321	85	2	1	318	2	322	2.03e-176	501

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454050	AT4G28880.3	70.883	419	107	4	1	410	1	413	0.0	611
Msa1454050	AT4G28880.2	70.883	419	107	4	1	410	1	413	0.0	611
Msa1454050	AT4G28880.1	70.883	419	107	4	1	410	1	413	0.0	611
Msa1454050	AT4G28880.4	70.883	419	107	4	1	410	1	413	0.0	611
Msa1454050	AT4G28860.1	71.801	422	102	7	1	416	1	411	0.0	606
Msa1454050	AT4G28860.2	79.339	363	68	3	1	358	1	361	0.0	602
Msa1454050	AT4G26100.1	72.000	325	90	1	1	325	1	324	1.94e-179	508
Msa1454050	AT5G57015.1	71.875	320	90	0	1	320	1	320	1.63e-177	503
Msa1454050	AT1G72710.1	69.275	345	103	3	1	345	1	342	1.17e-176	502
Msa1454050	AT2G19470.1	68.452	336	96	3	1	327	1	335	2.88e-175	497

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Find 152 sgRNAs with CRISPR-Local

Find 410 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCGGATAACTTTCTCA+TGG	0.575825	tig0035015:+6362	Msa1454050:CDS
AAACCGGATAACTTTCTCAT+GGG	0.561522	tig0035015:+6363	Msa1454050:CDS
AAATGAATTCAACACGCGGA+CGG	0.563130	tig0035015:-4365	None:intergenic
AAATTGGTCCTTCAAGCAGT+CGG	0.622441	tig0035015:+8604	Msa1454050:CDS
AACTAAAATCTTAATGTCAC+AGG	0.497589	tig0035015:-10323	None:intergenic
AAGATCGGAAGTGGATCCTT+CGG	0.526716	tig0035015:+4535	Msa1454050:CDS
AAGATGGAGTGGTGTAGACG+GGG	0.662328	tig0035015:+6002	Msa1454050:CDS
AAGCTCTATAATATTCTTCA+AGG	0.485336	tig0035015:+5435	Msa1454050:CDS
AAGCTGCAAACCATAGGCAT+GGG	0.597512	tig0035015:+8568	Msa1454050:CDS
AAGTACGAAGCAAACTCTAC+AGG	0.544026	tig0035015:-7298	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAAATGAATTCAACACG+CGG	-	tig0035015:4372-4391	None:intergenic	30.0%
AAGTATGTTTGGTATCACTA+TGG	-	tig0035015:5004-5023	None:intergenic	30.0%
CTCTATAATATTCTTCAAGG+AGG	+	tig0035015:5438-5457	Msa1454050:CDS	30.0%
AATGTTGTCAATTGCAGATT+GGG	-	tig0035015:5852-5871	None:intergenic	30.0%
ACTGACTAAAAAACATCACA+AGG	-	tig0035015:6173-6192	None:intergenic	30.0%
ATAGAGTATGTGCATTCTAA+AGG	+	tig0035015:6321-6340	Msa1454050:CDS	30.0%
TAGAGTATGTGCATTCTAAA+GGG	+	tig0035015:6322-6341	Msa1454050:CDS	30.0%
ATCAGTCAATTCTAAATGCA+GGG	-	tig0035015:6440-6459	None:intergenic	30.0%
AGAAAGTTCAAAACCTGTAT+GGG	-	tig0035015:6598-6617	None:intergenic	30.0%
TAGAAAGTTCAAAACCTGTA+TGG	-	tig0035015:6599-6618	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0035015	gene	4304	10419	4304	ID=Msa1454050;Name=Msa1454050
tig0035015	mRNA	4304	10419	4304	ID=Msa1454050-mRNA-1;Parent=Msa1454050;Name=Msa1454050-mRNA-1;_AED=0.19;_eAED=0.19;_QI=192\|0.92\|0.93\|1\|0.92\|0.93\|15\|495\|427
tig0035015	exon	4304	4571	4304	ID=Msa1454050-mRNA-1:exon:19475;Parent=Msa1454050-mRNA-1
tig0035015	exon	4685	4725	4685	ID=Msa1454050-mRNA-1:exon:19476;Parent=Msa1454050-mRNA-1
tig0035015	exon	5393	5462	5393	ID=Msa1454050-mRNA-1:exon:19477;Parent=Msa1454050-mRNA-1
tig0035015	exon	5986	6134	5986	ID=Msa1454050-mRNA-1:exon:19478;Parent=Msa1454050-mRNA-1
tig0035015	exon	6312	6407	6312	ID=Msa1454050-mRNA-1:exon:19479;Parent=Msa1454050-mRNA-1
tig0035015	exon	6533	6603	6533	ID=Msa1454050-mRNA-1:exon:19480;Parent=Msa1454050-mRNA-1
tig0035015	exon	6795	6856	6795	ID=Msa1454050-mRNA-1:exon:19481;Parent=Msa1454050-mRNA-1
tig0035015	exon	6942	7005	6942	ID=Msa1454050-mRNA-1:exon:19482;Parent=Msa1454050-mRNA-1
tig0035015	exon	7113	7197	7113	ID=Msa1454050-mRNA-1:exon:19483;Parent=Msa1454050-mRNA-1
tig0035015	exon	7280	7406	7280	ID=Msa1454050-mRNA-1:exon:19484;Parent=Msa1454050-mRNA-1
tig0035015	exon	7886	7968	7886	ID=Msa1454050-mRNA-1:exon:19485;Parent=Msa1454050-mRNA-1
tig0035015	exon	8067	8124	8067	ID=Msa1454050-mRNA-1:exon:19486;Parent=Msa1454050-mRNA-1
tig0035015	exon	8208	8323	8208	ID=Msa1454050-mRNA-1:exon:19487;Parent=Msa1454050-mRNA-1
tig0035015	exon	8483	8632	8483	ID=Msa1454050-mRNA-1:exon:19488;Parent=Msa1454050-mRNA-1
tig0035015	exon	9889	10419	9889	ID=Msa1454050-mRNA-1:exon:19489;Parent=Msa1454050-mRNA-1
tig0035015	five_prime_UTR	4304	4495	4304	ID=Msa1454050-mRNA-1:five_prime_utr;Parent=Msa1454050-mRNA-1
tig0035015	CDS	4496	4571	4496	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	4685	4725	4685	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	5393	5462	5393	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	5986	6134	5986	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	6312	6407	6312	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	6533	6603	6533	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	6795	6856	6795	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	6942	7005	6942	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	7113	7197	7113	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	7280	7406	7280	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	7886	7968	7886	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	8067	8124	8067	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	8208	8323	8208	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	8483	8632	8483	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	CDS	9889	9924	9889	ID=Msa1454050-mRNA-1:cds;Parent=Msa1454050-mRNA-1
tig0035015	three_prime_UTR	9925	10419	9925	ID=Msa1454050-mRNA-1:three_prime_utr;Parent=Msa1454050-mRNA-1

Gene Sequence

>Msa1454050

ATGGATCGAATCATCGGTGATAAATTCAAGTTAGGACGCAAGATCGGAAGTGGATCCTTCGGTGAAATCTATCTCGCTACTCATATTGATACCTTCGAGATCGTTGCTGTCAAGATCGAGACTGGTAAAACGAAGCATCCACAATTGCTTTATGAAGCCAAGCTCTATAATATTCTTCAAGGAGGAAGTGGTATCCCAAGCATAAGATGGAGTGGTGTAGACGGGGAAGACAATGTGCTTGTTATGGATTTGTTGGGCCCTAGTCTTGAGGATCTTTTTGTTTACTGTGGAAGAAAATTTTCCCTCAAAACTGTCTTGATGTTGGCTGATCAAATGATGACCAGAATAGAGTATGTGCATTCTAAAGGGTTCCTCCATAGGGATATAAAACCGGATAACTTTCTCATGGGACTTGGTCGGAAGGCAAACCTGGTATATGTAATTGATTTTGGGCTTGCAAAACGATATCGGGACTCCACAACCAATCGCCACATCCCATACAGGGAGAACAAAAACTTAACCGGGACTGCGCGTTATGCAAGCTGCAATACTCATCTTGGGATTGAACAAAGCCGCCGGGATGATTTGGAGTCTCTTGGATACGTGCTTTTGTACTTCCTCAGAGGAAGCCTTCCCTGGCAAAACCTAAAGGCTGCGACAAAGAAGCAGAAGTATGATAAAATATGTGAAAAGAAAGTATCAACTCCTATTGAGGTTTTATGCAAATCCCATCCTGTAGAGTTTGCTTCGTACTTCCATTACTGCCATTCTTTGACCTTTGATCAGCGACCTGATTATGGATTCTTGAGGCGCCTATTTCGGGAGTTATTTGCTCGAGAAGGTTACAAATTTGATTATGTATTTGATTGGACAATTTTGAAGTACCAGCAGTCACAAAAGAGTAGAGCCCAGCCTCACATATCCCCGGTTCCTGTAGCGAGCAACAATCGGGCAATGCCCATGGATATTGATAACCAACAAGGGGATGTCTCAGCGGAGCAGATTAGATCAGACAATGCCGCTTCTTCCGGTGTTAAGTTCCAGTTTAAATCATCAGGCGGTAAAAATTTGGCTTCTGAGAATCATCTTGGCAAGAATATTTTTGGTGAAGCAACTATCCCTTCTACTTCACACTCTTTTGAAGCATCAAGAAGGAACTCCTTGAAGCCTTCCGCGTCCACTGAAGCTGCAAACCATAGGCATGGGCAAGGGAGCAGTAAAATTGGTCCTTCAAGCAGTCGGATGAATGTCTCTTTTTTTTCTAATCTATATGGCAATGGATGA

Protein sequence

>Msa1454050

MDRIIGDKFKLGRKIGSGSFGEIYLATHIDTFEIVAVKIETGKTKHPQLLYEAKLYNILQGGSGIPSIRWSGVDGEDNVLVMDLLGPSLEDLFVYCGRKFSLKTVLMLADQMMTRIEYVHSKGFLHRDIKPDNFLMGLGRKANLVYVIDFGLAKRYRDSTTNRHIPYRENKNLTGTARYASCNTHLGIEQSRRDDLESLGYVLLYFLRGSLPWQNLKAATKKQKYDKICEKKVSTPIEVLCKSHPVEFASYFHYCHSLTFDQRPDYGFLRRLFRELFAREGYKFDYVFDWTILKYQQSQKSRAQPHISPVPVASNNRAMPMDIDNQQGDVSAEQIRSDNAASSGVKFQFKSSGGKNLASENHLGKNIFGEATIPSTSHSFEASRRNSLKPSASTEAANHRHGQGSSKIGPSSSRMNVSFFSNLYGNG*