AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1454650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454650 MtrunA17_Chr4g0043891 92.800 125 2 2 1 125 1 118 3.47e-78 231
Msa1454650 MtrunA17_Chr8g0392991 60.345 116 35 3 6 121 94 198 3.71e-37 129
Msa1454650 MtrunA17_Chr7g0238431 71.429 70 20 0 1 70 1 70 1.10e-31 111
Msa1454650 MtrunA17_Chr5g0436661 39.552 134 71 2 8 131 33 166 3.01e-28 102
Msa1454650 MtrunA17_Chr5g0405351 67.164 67 21 1 4 69 26 92 2.02e-27 102
Msa1454650 MtrunA17_Chr1g0153171 68.750 64 20 0 7 70 89 152 8.67e-27 99.8
Msa1454650 MtrunA17_Chr8g0385441 84.615 52 8 0 19 70 93 144 1.43e-26 97.4
Msa1454650 MtrunA17_Chr3g0125111 84.615 52 8 0 19 70 91 142 1.55e-26 97.4
Msa1454650 MtrunA17_Chr4g0031341 66.667 63 21 0 8 70 25 87 5.19e-26 98.2
Msa1454650 MtrunA17_Chr7g0223471 47.761 67 35 0 3 69 42 108 1.60e-18 80.1
Msa1454650 MtrunA17_Chr1g0212511 46.341 82 38 1 8 89 85 160 3.25e-18 77.8
Msa1454650 MtrunA17_Chr2g0283141 44.928 69 38 0 3 71 50 118 6.92e-18 78.6
Msa1454650 MtrunA17_Chr3g0144021 42.623 61 35 0 8 68 82 142 8.29e-14 65.5
Msa1454650 MtrunA17_Chr6g0460691 38.961 77 47 0 7 83 31 107 2.71e-13 65.1
Msa1454650 MtrunA17_Chr7g0222981 36.559 93 49 1 8 100 68 150 4.39e-13 62.8
Msa1454650 MtrunA17_Chr7g0254251 45.098 51 28 0 7 57 27 77 5.03e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454650 AT3G18010.1 53.659 123 45 2 6 125 74 187 3.84e-36 127
Msa1454650 AT2G28610.1 65.714 70 24 0 1 70 1 70 5.82e-30 108
Msa1454650 AT5G59340.1 64.706 68 24 0 6 73 12 79 1.14e-27 103
Msa1454650 AT3G11260.1 59.701 67 27 0 8 74 24 90 1.03e-26 98.6
Msa1454650 AT2G17950.1 60.759 79 30 1 4 81 34 112 1.82e-26 100
Msa1454650 AT1G46480.2 63.768 69 24 1 2 70 85 152 2.91e-26 97.8
Msa1454650 AT2G01500.1 74.545 55 14 0 8 62 61 115 7.01e-26 98.6
Msa1454650 AT1G46480.1 63.768 69 24 1 2 70 85 152 1.36e-25 97.4
Msa1454650 AT5G05770.1 59.375 64 26 0 8 71 29 92 1.59e-23 88.6
Msa1454650 AT2G33880.1 44.286 70 39 0 3 72 50 119 2.04e-17 77.4
Msa1454650 AT2G33880.2 44.286 70 39 0 3 72 50 119 2.67e-17 77.0
Msa1454650 AT5G45980.1 44.776 67 37 0 3 69 50 116 4.59e-16 73.2
Msa1454650 AT4G35550.1 48.387 62 32 0 8 69 99 160 8.59e-16 71.6
Msa1454650 AT1G20700.2 45.000 60 33 0 8 67 33 92 2.44e-14 65.9
Msa1454650 AT1G20700.3 45.000 60 33 0 8 67 95 154 8.45e-14 65.5
Msa1454650 AT1G20700.1 45.000 60 33 0 8 67 95 154 1.04e-13 65.5
Msa1454650 AT3G03660.1 49.057 53 27 0 7 59 32 84 3.58e-13 64.7
Msa1454650 AT3G03660.2 49.057 53 27 0 7 59 32 84 5.44e-13 64.3
Msa1454650 AT3G03660.4 49.057 53 27 0 7 59 32 84 7.95e-13 63.9
Msa1454650 AT3G03660.3 49.057 53 27 0 7 59 32 84 1.09e-12 63.9
Msa1454650 AT5G17810.1 43.396 53 30 0 7 59 20 72 2.14e-11 59.7
Msa1454650 AT5G17810.2 43.396 53 30 0 7 59 20 72 2.14e-11 59.7
Msa1454650 AT5G17810.3 43.396 53 30 0 7 59 20 72 2.26e-11 59.7

Find 28 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTCTCGCCTTGTGATTC+TGG 0.194023 tig0035337:+22822 None:intergenic
GCTTCGCAGCTTCGACAATT+TGG 0.206061 tig0035337:-22878 Msa1454650:CDS
ATTTCTCTTGTCTTTGTTGA+TGG 0.263889 tig0035337:-22125 None:intergenic
AGAGAAAGAGAAGTACGTTA+TGG 0.359866 tig0035337:-22735 Msa1454650:CDS
CGACAATTTGGAAAGATTGA+AGG 0.396848 tig0035337:-22866 Msa1454650:CDS
GAGAAAGAGAAGTACGTTAT+GGG 0.401887 tig0035337:-22734 Msa1454650:CDS
GAAGCTGCGAAGCTATTTGT+TGG 0.428703 tig0035337:+22888 None:intergenic
CTTGCCTATACAATTCTTCA+AGG 0.428836 tig0035337:+22939 None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG 0.431151 tig0035337:-22943 Msa1454650:CDS
GTTGGACTCCACCTTGAACT+TGG 0.444756 tig0035337:+22980 None:intergenic
CTTGAGATGGAGGAAACAAT+TGG 0.464032 tig0035337:-22782 Msa1454650:CDS
TGCATCCTCAACCAAGTTCA+AGG 0.472729 tig0035337:-22991 Msa1454650:CDS
CTCCACCTTGAACTTGGTTG+AGG 0.502185 tig0035337:+22986 None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG 0.503667 tig0035337:+22905 None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG 0.506780 tig0035337:+22161 None:intergenic
TCAAGGACTAGTAATTGAAC+TGG 0.509163 tig0035337:+22956 None:intergenic
GAAGCGTCGTCGCCTTGAGA+TGG 0.530642 tig0035337:-22795 Msa1454650:CDS
ACTAGTAATTGAACTGGTGT+TGG 0.537149 tig0035337:+22962 None:intergenic
CTTGAAGAATTGTATAGGCA+AGG 0.540070 tig0035337:-22938 Msa1454650:CDS
TCAGAAAAGCAAGAAGCTGC+AGG 0.540109 tig0035337:-22707 Msa1454650:intron
CAATTGTTTCCTCCATCTCA+AGG 0.548253 tig0035337:+22783 None:intergenic
GCGTCGTCGCCTTGAGATGG+AGG 0.576407 tig0035337:-22792 Msa1454650:CDS
TTGAAGAATTGTATAGGCAA+GGG 0.590766 tig0035337:-22937 Msa1454650:CDS
AAACTGCAGTGAACAAGCAG+AGG 0.632631 tig0035337:-22154 Msa1454650:CDS
AGTTAAAGAGACAAAGAAGT+GGG 0.667079 tig0035337:-22187 Msa1454650:CDS
CTATTGGTTCCAGAATCACA+AGG 0.685006 tig0035337:-22831 Msa1454650:CDS
GAGTTAAAGAGACAAAGAAG+TGG 0.689037 tig0035337:-22188 Msa1454650:CDS
ATCCTCAACCAAGTTCAAGG+TGG 0.762805 tig0035337:-22988 Msa1454650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTCTTTCAAATA+TGG + tig0035337:22759-22778 None:intergenic 10.0%
!!! AAATTTTTTTCTTTCAAATA+TGG + tig0035337:22759-22778 None:intergenic 10.0%
!!! TTGTTGTTTATTCAATTTAA+TGG - tig0035337:22780-22799 Msa1454650:CDS 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG - tig0035337:22811-22830 Msa1454650:CDS 15.0%
!!! TTGTTGTTTATTCAATTTAA+TGG - tig0035337:22780-22799 Msa1454650:CDS 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG - tig0035337:22811-22830 Msa1454650:CDS 15.0%
!! TCATGAGTGAAATATAATAT+AGG + tig0035337:22497-22516 None:intergenic 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG - tig0035337:22553-22572 Msa1454650:intron 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG + tig0035337:22619-22638 None:intergenic 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG + tig0035337:22620-22639 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG + tig0035337:22652-22671 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG + tig0035337:22735-22754 None:intergenic 20.0%
!! TCATGAGTGAAATATAATAT+AGG + tig0035337:22497-22516 None:intergenic 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG - tig0035337:22553-22572 Msa1454650:intron 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG + tig0035337:22619-22638 None:intergenic 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG + tig0035337:22620-22639 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG + tig0035337:22652-22671 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG + tig0035337:22735-22754 None:intergenic 20.0%
! AAGGAAAAAATGTGTTCTAT+TGG - tig0035337:22273-22292 Msa1454650:intron 25.0%
! TACAGCTAGATATTATCTTT+AGG - tig0035337:22438-22457 Msa1454650:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG - tig0035337:22439-22458 Msa1454650:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG - tig0035337:22449-22468 Msa1454650:intron 25.0%
! AAATATCAATTTAGCCCATT+TGG - tig0035337:22602-22621 Msa1454650:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG + tig0035337:22653-22672 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG - tig0035337:22650-22669 Msa1454650:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG - tig0035337:22704-22723 Msa1454650:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG - tig0035337:22880-22899 Msa1454650:CDS 25.0%
! AAGGAAAAAATGTGTTCTAT+TGG - tig0035337:22273-22292 Msa1454650:intron 25.0%
! TACAGCTAGATATTATCTTT+AGG - tig0035337:22438-22457 Msa1454650:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG - tig0035337:22439-22458 Msa1454650:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG - tig0035337:22449-22468 Msa1454650:intron 25.0%
! AAATATCAATTTAGCCCATT+TGG - tig0035337:22602-22621 Msa1454650:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG + tig0035337:22653-22672 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG - tig0035337:22650-22669 Msa1454650:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG - tig0035337:22704-22723 Msa1454650:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG - tig0035337:22880-22899 Msa1454650:CDS 25.0%
TAGTCCTTGAAGAATTGTAT+AGG - tig0035337:22177-22196 Msa1454650:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG - tig0035337:22183-22202 Msa1454650:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG - tig0035337:22461-22480 Msa1454650:intron 30.0%
! AATAGCTTTTGTAAGGATCT+CGG - tig0035337:22560-22579 Msa1454650:intron 30.0%
ACACACACTTCAAATTGTAA+GGG + tig0035337:22850-22869 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG - tig0035337:22933-22952 Msa1454650:CDS 30.0%
TAGTCCTTGAAGAATTGTAT+AGG - tig0035337:22177-22196 Msa1454650:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG - tig0035337:22183-22202 Msa1454650:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG - tig0035337:22461-22480 Msa1454650:intron 30.0%
! AATAGCTTTTGTAAGGATCT+CGG - tig0035337:22560-22579 Msa1454650:intron 30.0%
ACACACACTTCAAATTGTAA+GGG + tig0035337:22850-22869 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG - tig0035337:22933-22952 Msa1454650:CDS 30.0%
ACTAGTAATTGAACTGGTGT+TGG + tig0035337:22161-22180 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG + tig0035337:22167-22186 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG + tig0035337:22184-22203 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG - tig0035337:22182-22201 Msa1454650:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG - tig0035337:22254-22273 Msa1454650:intron 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG - tig0035337:22385-22404 Msa1454650:intron 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG - tig0035337:22386-22405 Msa1454650:intron 35.0%
CACACACACTTCAAATTGTA+AGG + tig0035337:22851-22870 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG - tig0035337:22887-22906 Msa1454650:CDS 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG - tig0035337:22904-22923 Msa1454650:CDS 35.0%
GAGTTAAAGAGACAAAGAAG+TGG - tig0035337:22932-22951 Msa1454650:CDS 35.0%
ACTAGTAATTGAACTGGTGT+TGG + tig0035337:22161-22180 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG + tig0035337:22167-22186 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG + tig0035337:22184-22203 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG - tig0035337:22182-22201 Msa1454650:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG - tig0035337:22254-22273 Msa1454650:intron 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG - tig0035337:22385-22404 Msa1454650:intron 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG - tig0035337:22386-22405 Msa1454650:intron 35.0%
CACACACACTTCAAATTGTA+AGG + tig0035337:22851-22870 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG - tig0035337:22887-22906 Msa1454650:CDS 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG - tig0035337:22904-22923 Msa1454650:CDS 35.0%
GAGTTAAAGAGACAAAGAAG+TGG - tig0035337:22932-22951 Msa1454650:CDS 35.0%
! TGTTGGATTTGTTCAGCTGA+TGG + tig0035337:22218-22237 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG - tig0035337:22289-22308 Msa1454650:intron 40.0%
CAATTGTTTCCTCCATCTCA+AGG + tig0035337:22340-22359 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG - tig0035337:22338-22357 Msa1454650:intron 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG - tig0035337:22907-22926 Msa1454650:CDS 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG - tig0035337:22910-22929 Msa1454650:CDS 40.0%
TTGTTCACTGCAGTTTGAAG+TGG + tig0035337:22962-22981 None:intergenic 40.0%
! TGTTGGATTTGTTCAGCTGA+TGG + tig0035337:22218-22237 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG - tig0035337:22289-22308 Msa1454650:intron 40.0%
CAATTGTTTCCTCCATCTCA+AGG + tig0035337:22340-22359 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG - tig0035337:22338-22357 Msa1454650:intron 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG - tig0035337:22907-22926 Msa1454650:CDS 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG - tig0035337:22910-22929 Msa1454650:CDS 40.0%
TTGTTCACTGCAGTTTGAAG+TGG + tig0035337:22962-22981 None:intergenic 40.0%
TGCATCCTCAACCAAGTTCA+AGG - tig0035337:22129-22148 Msa1454650:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG - tig0035337:22132-22151 Msa1454650:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG + tig0035337:22235-22254 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG - tig0035337:22413-22432 Msa1454650:intron 45.0%
AAACTGCAGTGAACAAGCAG+AGG - tig0035337:22966-22985 Msa1454650:CDS 45.0%
TGCATCCTCAACCAAGTTCA+AGG - tig0035337:22129-22148 Msa1454650:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG - tig0035337:22132-22151 Msa1454650:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG + tig0035337:22235-22254 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG - tig0035337:22413-22432 Msa1454650:intron 45.0%
AAACTGCAGTGAACAAGCAG+AGG - tig0035337:22966-22985 Msa1454650:CDS 45.0%
CTCCACCTTGAACTTGGTTG+AGG + tig0035337:22137-22156 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG + tig0035337:22143-22162 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG - tig0035337:22242-22261 Msa1454650:intron 50.0%
CTCCACCTTGAACTTGGTTG+AGG + tig0035337:22137-22156 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG + tig0035337:22143-22162 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG - tig0035337:22242-22261 Msa1454650:intron 50.0%
GCTCTCTCGCCTTGTGATTC+TGG + tig0035337:22301-22320 None:intergenic 55.0%
GCTCTCTCGCCTTGTGATTC+TGG + tig0035337:22301-22320 None:intergenic 55.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG - tig0035337:22325-22344 Msa1454650:intron 60.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG - tig0035337:22325-22344 Msa1454650:intron 60.0%
GCGTCGTCGCCTTGAGATGG+AGG - tig0035337:22328-22347 Msa1454650:intron 65.0%
GCGTCGTCGCCTTGAGATGG+AGG - tig0035337:22328-22347 Msa1454650:intron 65.0%
Chromosome Type Strat End Strand Name
tig0035337 gene 22128 23014 22128 ID=Msa1454650;Name=Msa1454650
tig0035337 mRNA 22128 23014 22128 ID=Msa1454650-mRNA-1;Parent=Msa1454650;Name=Msa1454650-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|2|0|133
tig0035337 exon 22708 23014 22708 ID=Msa1454650-mRNA-1:exon:19885;Parent=Msa1454650-mRNA-1
tig0035337 exon 22128 22222 22128 ID=Msa1454650-mRNA-1:exon:19884;Parent=Msa1454650-mRNA-1
tig0035337 CDS 22708 23014 22708 ID=Msa1454650-mRNA-1:cds;Parent=Msa1454650-mRNA-1
tig0035337 CDS 22128 22222 22128 ID=Msa1454650-mRNA-1:cds;Parent=Msa1454650-mRNA-1
Gene Sequence

>Msa1454650

ATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGA

Protein sequence

>Msa1454650

MHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAETGGGVKETKKWATTSNCSEQAEVCNFSCLC*