AlfalfaGEDB | Msa1454650

M. sativa cultivar zhongmu-4 / Msa1454650

KEGG: -
GO: GO:0000003 GO:0001558 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0009653 GO:0009790 GO:0009791 GO:0009889 GO:0009908 GO:0009965 GO:0009987 GO:0010016 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0040008 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048314 GO:0048353 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048441 GO:0048444 GO:0048445 GO:0048446 GO:0048465 GO:0048467 GO:0048481 GO:0048482 GO:0048598 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051252 GO:0051301 GO:0051510 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454650	sp\|Q6X7K0\|WOX1_ARATH	53.659	123	45	2	6	125	74	187	3.77e-35	127

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454650	A0A072UYB2	94.737	133	2	1	1	133	57	184	3.26e-86	258

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TFs/TRs:

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Gene ID	Type	Classification
Msa1454650	TF	HB-WOX

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454650	MtrunA17_Chr4g0043891	92.800	125	2	2	1	125	1	118	3.47e-78	231
Msa1454650	MtrunA17_Chr8g0392991	60.345	116	35	3	6	121	94	198	3.71e-37	129
Msa1454650	MtrunA17_Chr7g0238431	71.429	70	20	0	1	70	1	70	1.10e-31	111
Msa1454650	MtrunA17_Chr5g0436661	39.552	134	71	2	8	131	33	166	3.01e-28	102
Msa1454650	MtrunA17_Chr5g0405351	67.164	67	21	1	4	69	26	92	2.02e-27	102
Msa1454650	MtrunA17_Chr1g0153171	68.750	64	20	0	7	70	89	152	8.67e-27	99.8
Msa1454650	MtrunA17_Chr8g0385441	84.615	52	8	0	19	70	93	144	1.43e-26	97.4
Msa1454650	MtrunA17_Chr3g0125111	84.615	52	8	0	19	70	91	142	1.55e-26	97.4
Msa1454650	MtrunA17_Chr4g0031341	66.667	63	21	0	8	70	25	87	5.19e-26	98.2
Msa1454650	MtrunA17_Chr7g0223471	47.761	67	35	0	3	69	42	108	1.60e-18	80.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454650	AT3G18010.1	53.659	123	45	2	6	125	74	187	3.84e-36	127
Msa1454650	AT2G28610.1	65.714	70	24	0	1	70	1	70	5.82e-30	108
Msa1454650	AT5G59340.1	64.706	68	24	0	6	73	12	79	1.14e-27	103
Msa1454650	AT3G11260.1	59.701	67	27	0	8	74	24	90	1.03e-26	98.6
Msa1454650	AT2G17950.1	60.759	79	30	1	4	81	34	112	1.82e-26	100
Msa1454650	AT1G46480.2	63.768	69	24	1	2	70	85	152	2.91e-26	97.8
Msa1454650	AT2G01500.1	74.545	55	14	0	8	62	61	115	7.01e-26	98.6
Msa1454650	AT1G46480.1	63.768	69	24	1	2	70	85	152	1.36e-25	97.4
Msa1454650	AT5G05770.1	59.375	64	26	0	8	71	29	92	1.59e-23	88.6
Msa1454650	AT2G33880.1	44.286	70	39	0	3	72	50	119	2.04e-17	77.4

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Find 28 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGCAGTGAACAAGCAG+AGG	0.632631	tig0035337:-22154	Msa1454650:CDS
ACTAGTAATTGAACTGGTGT+TGG	0.537149	tig0035337:+22962	None:intergenic
AGAGAAAGAGAAGTACGTTA+TGG	0.359866	tig0035337:-22735	Msa1454650:CDS
AGTTAAAGAGACAAAGAAGT+GGG	0.667079	tig0035337:-22187	Msa1454650:CDS
ATCCTCAACCAAGTTCAAGG+TGG	0.762805	tig0035337:-22988	Msa1454650:CDS
ATTTCTCTTGTCTTTGTTGA+TGG	0.263889	tig0035337:-22125	None:intergenic
CAATTGTTTCCTCCATCTCA+AGG	0.548253	tig0035337:+22783	None:intergenic
CGACAATTTGGAAAGATTGA+AGG	0.396848	tig0035337:-22866	Msa1454650:CDS
CTATTGGTTCCAGAATCACA+AGG	0.685006	tig0035337:-22831	Msa1454650:CDS
CTCCACCTTGAACTTGGTTG+AGG	0.502185	tig0035337:+22986	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGTCCTTGAAGAATTGTAT+AGG	-	tig0035337:22177-22196	Msa1454650:CDS	30.0%
TTGAAGAATTGTATAGGCAA+GGG	-	tig0035337:22183-22202	Msa1454650:CDS	30.0%
ACACACACTTCAAATTGTAA+GGG	+	tig0035337:22850-22869	None:intergenic	30.0%
AGTTAAAGAGACAAAGAAGT+GGG	-	tig0035337:22933-22952	Msa1454650:CDS	30.0%
TAGTCCTTGAAGAATTGTAT+AGG	-	tig0035337:22177-22196	Msa1454650:CDS	30.0%
TTGAAGAATTGTATAGGCAA+GGG	-	tig0035337:22183-22202	Msa1454650:CDS	30.0%
ACACACACTTCAAATTGTAA+GGG	+	tig0035337:22850-22869	None:intergenic	30.0%
AGTTAAAGAGACAAAGAAGT+GGG	-	tig0035337:22933-22952	Msa1454650:CDS	30.0%
ACTAGTAATTGAACTGGTGT+TGG	+	tig0035337:22161-22180	None:intergenic	35.0%
CTTGCCTATACAATTCTTCA+AGG	+	tig0035337:22184-22203	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0035337	gene	22128	23014	22128	ID=Msa1454650;Name=Msa1454650
tig0035337	mRNA	22128	23014	22128	ID=Msa1454650-mRNA-1;Parent=Msa1454650;Name=Msa1454650-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0\|0\|1\|1\|1\|2\|0\|133
tig0035337	exon	22708	23014	22708	ID=Msa1454650-mRNA-1:exon:19885;Parent=Msa1454650-mRNA-1
tig0035337	exon	22128	22222	22128	ID=Msa1454650-mRNA-1:exon:19884;Parent=Msa1454650-mRNA-1
tig0035337	CDS	22708	23014	22708	ID=Msa1454650-mRNA-1:cds;Parent=Msa1454650-mRNA-1
tig0035337	CDS	22128	22222	22128	ID=Msa1454650-mRNA-1:cds;Parent=Msa1454650-mRNA-1

Gene Sequence

>Msa1454650

ATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGA

Protein sequence

>Msa1454650

MHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAETGGGVKETKKWATTSNCSEQAEVCNFSCLC*