AlfalfaGEDB | Msa1455540

M. sativa cultivar zhongmu-4 / Msa1455540

KEGG: K03321|K08065
GO: -
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1455540	AET00786.2	87.634	186	18	3	1	184	1	183	7.41e-90	271

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1455540	sp\|Q9SFD8\|NFYB9_ARATH	44.737	114	59	1	6	115	49	162	6.27e-24	97.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1455540	G7KEG5	87.634	186	18	3	1	184	1	183	3.54e-90	271

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TFs/TRs:

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Gene ID	Type	Classification
Msa1455540	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1455540	MtrunA17_Chr5g0446601	86.486	185	20	3	2	184	10	191	2.22e-92	267
Msa1455540	MtrunA17_Chr5g0446561	80.392	102	14	2	84	184	1	97	9.74e-30	105
Msa1455540	MtrunA17_Chr4g0076321	45.946	111	51	3	3	107	7	114	3.77e-25	94.7
Msa1455540	MtrunA17_Chr4g0076381	46.296	108	54	1	4	107	46	153	6.01e-25	97.1
Msa1455540	MtrunA17_Chr1g0165041	44.144	111	55	1	16	119	6	116	3.89e-24	93.6
Msa1455540	MtrunA17_Chr4g0067091	47.312	93	49	0	13	105	19	111	4.80e-23	91.7
Msa1455540	MtrunA17_Chr1g0191981	47.826	92	48	0	13	104	1	92	5.76e-23	89.0
Msa1455540	MtrunA17_Chr3g0102351	47.778	90	47	0	16	105	27	116	7.05e-23	90.5
Msa1455540	MtrunA17_Chr5g0446491	47.778	90	47	0	16	105	23	112	7.12e-23	90.1
Msa1455540	MtrunA17_Chr2g0296321	46.154	91	49	0	15	105	65	155	4.64e-22	89.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1455540	AT1G21970.1	44.737	114	59	1	6	115	49	162	6.38e-25	97.4
Msa1455540	AT5G47670.2	50.000	90	45	0	16	105	29	118	1.80e-24	95.1
Msa1455540	AT5G47670.1	50.000	90	45	0	16	105	58	147	2.48e-24	95.5
Msa1455540	AT5G47670.3	50.000	90	45	0	16	105	58	147	2.48e-24	95.5
Msa1455540	AT5G47640.1	50.000	90	45	0	16	105	27	116	4.67e-23	91.3
Msa1455540	AT2G47810.1	44.231	104	57	1	1	104	37	139	1.57e-22	89.0
Msa1455540	AT4G14540.1	46.392	97	49	1	9	105	17	110	2.13e-22	88.6
Msa1455540	AT2G37060.2	43.137	102	53	2	8	105	23	123	3.19e-21	85.9
Msa1455540	AT2G37060.3	43.137	102	53	2	8	105	23	123	3.19e-21	85.9
Msa1455540	AT2G37060.1	43.137	102	53	2	8	105	23	123	3.19e-21	85.9

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Find 48 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGCACCCATTTCTGT+TGG	0.484335	tig0036297:-6617	Msa1455540:intergenic
AAATCCTGTGGCAGCGGCGC+TGG	0.458062	tig0036297:-6548	None:intergenic
AAATTGCACCCATTTCTGTT+GGG	0.486823	tig0036297:-6616	Msa1455540:intergenic
AACACGAAATTTGGATCCAA+CGG	0.532134	tig0036297:-6587	Msa1455540:intergenic
ACACGAAATTTGGATCCAAC+GGG	0.476879	tig0036297:-6586	Msa1455540:intergenic
AGCCTATGCACATTGGCAAT+GGG	0.499486	tig0036297:-6248	Msa1455540:intergenic
AGCGGCGCTGGTGGAGGTGG+AGG	0.431125	tig0036297:-6536	Msa1455540:intergenic
AGTGCAACCAGCTAGTTCGA+GGG	0.693334	tig0036297:+6199	Msa1455540:CDS
ATCTAACTTGCAATAATGGT+TGG	0.494716	tig0036297:-6372	Msa1455540:intergenic
ATGCACATTGGCAATGGGAA+AGG	0.554205	tig0036297:-6243	Msa1455540:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATAACCGATGAAAGCA+AGG	+	tig0036297:6298-6317	Msa1455540:CDS	30.0%
CATAATTTCCTTGCTTTCAT+CGG	-	tig0036297:6309-6328	Msa1455540:intergenic	30.0%
CGATGAAAGCAAGGAAATTA+TGG	+	tig0036297:6307-6326	Msa1455540:CDS	35.0%
TTATGAGCTTGAAAATCCTG+TGG	-	tig0036297:6563-6582	Msa1455540:intergenic	35.0%
AACACGAAATTTGGATCCAA+CGG	-	tig0036297:6590-6609	Msa1455540:intergenic	35.0%
AAATTGCACCCATTTCTGTT+GGG	-	tig0036297:6619-6638	Msa1455540:intergenic	35.0%
AAAATTGCACCCATTTCTGT+TGG	-	tig0036297:6620-6639	Msa1455540:intergenic	35.0%
TTTCATCAGCCTATGCACAT+TGG	-	tig0036297:6258-6277	Msa1455540:intergenic	40.0%
CACGAAAAAGTTGGATGTAG+CGG	-	tig0036297:6478-6497	Msa1455540:intergenic	40.0%
GAGAACGATCACGAAAAAGT+TGG	-	tig0036297:6487-6506	Msa1455540:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0036297	gene	6185	6739	6185	ID=Msa1455540;Name=Msa1455540
tig0036297	mRNA	6185	6739	6185	ID=Msa1455540-mRNA-1;Parent=Msa1455540;Name=Msa1455540-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|184
tig0036297	exon	6185	6739	6185	ID=Msa1455540-mRNA-1:exon:20311;Parent=Msa1455540-mRNA-1
tig0036297	CDS	6185	6739	6185	ID=Msa1455540-mRNA-1:cds;Parent=Msa1455540-mRNA-1

Gene Sequence

>Msa1455540

ATGTTAGAGGATGGAGTGCAACCAGCTAGTTCGAGGGTGCAAGAAGAATCTGCAAGAGCCTTTCCCATTGCCAATGTGCATAGGCTGATGAAAAAAGCTCTTCCTAGACATGCAAAAATAACCGATGAAAGCAAGGAAATTATGGTGAAATATGCAGCTGAATTCATTAGCTTTGTTACGGCTGAGGCCAACCATTATTGCAAGTTAGATTATAGGACAACAGTTACTGCTGAAGATTTGTTAATTACTATGCACAAATTGGGTTTTGACGTCTATGCTGAGTATTCCCTCCGCTACATCCAACTTTTTCGTGATCGTTCTCGTGTAGGTCAATATGCTCCCCCACCTCCACCTCCACCTCCACCAGCGCCGCTGCCACAGGATTTTCAAGCTCATAATTTCCCGTTGGATCCAAATTTCGTGTTTGCTGACCCAACAGAAATGGGTGCAATTTTAGGAGATGATGATGATGATAGAGAACTTGGTGGTGGTGGTGATGGCAACTCAAGTGATGGAAGCTTTGATGTTATGACTTTTCTTAATAGTGATGACTAA

Protein sequence

>Msa1455540

MLEDGVQPASSRVQEESARAFPIANVHRLMKKALPRHAKITDESKEIMVKYAAEFISFVTAEANHYCKLDYRTTVTAEDLLITMHKLGFDVYAEYSLRYIQLFRDRSRVGQYAPPPPPPPPPAPLPQDFQAHNFPLDPNFVFADPTEMGAILGDDDDDRELGGGGDGNSSDGSFDVMTFLNSDD*