AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1456660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456660 MtrunA17_Chr5g0415291 86.722 241 30 1 1 241 1 239 2.92e-157 436
Msa1456660 MtrunA17_Chr8g0363921 61.509 265 64 8 5 240 22 277 3.85e-96 282
Msa1456660 MtrunA17_Chr8g0339091 79.121 91 19 0 105 195 282 372 8.40e-45 156
Msa1456660 MtrunA17_Chr7g0234761 63.492 126 42 1 77 202 284 405 1.90e-44 155
Msa1456660 MtrunA17_Chr3g0144641 61.654 133 40 3 74 196 148 279 1.23e-42 149
Msa1456660 MtrunA17_Chr4g0035101 50.802 187 70 6 23 202 61 232 3.11e-42 146
Msa1456660 MtrunA17_Chr5g0421111 58.779 131 50 3 93 220 343 472 2.65e-40 145
Msa1456660 MtrunA17_Chr8g0365941 64.865 111 38 1 93 202 283 393 4.09e-40 143
Msa1456660 MtrunA17_Chr8g0385911 80.952 84 15 1 102 185 134 216 2.40e-39 138
Msa1456660 MtrunA17_Chr3g0131771 82.278 79 14 0 107 185 140 218 2.92e-39 138
Msa1456660 MtrunA17_Chr1g0152131 70.968 93 26 1 94 185 239 331 6.16e-38 137
Msa1456660 MtrunA17_Chr1g0174161 78.667 75 16 0 111 185 157 231 4.73e-36 130
Msa1456660 MtrunA17_Chr7g0258161 75.000 80 20 0 108 187 167 246 4.83e-35 127
Msa1456660 MtrunA17_Chr6g0484271 55.357 112 43 1 75 186 123 227 1.17e-34 125
Msa1456660 MtrunA17_Chr4g0074161 66.667 66 21 1 114 179 206 270 1.91e-20 89.4
Msa1456660 MtrunA17_Chr1g0190731 60.000 75 29 1 105 179 184 257 2.84e-20 88.2
Msa1456660 MtrunA17_Chr1g0182791 47.561 82 41 2 116 197 319 398 1.53e-15 75.5
Msa1456660 MtrunA17_Chr1g0184841 44.355 124 61 3 58 179 67 184 4.48e-15 72.8
Msa1456660 MtrunA17_Chr1g0193321 48.438 64 32 1 116 179 464 526 2.62e-14 72.0
Msa1456660 MtrunA17_Chr4g0032951 53.846 65 30 0 114 178 355 419 2.70e-14 71.6
Msa1456660 MtrunA17_Chr4g0014771 47.692 65 34 0 114 178 118 182 3.93e-13 65.9
Msa1456660 MtrunA17_Chr2g0329041 49.231 65 33 0 112 176 330 394 5.12e-13 67.8
Msa1456660 MtrunA17_Chr1g0174271 49.254 67 34 0 114 180 298 364 2.04e-12 65.9
Msa1456660 MtrunA17_Chr1g0153241 35.106 94 55 2 87 180 63 150 5.83e-12 63.9
Msa1456660 MtrunA17_Chr7g0258181 46.269 67 36 0 114 180 243 309 6.31e-12 64.3
Msa1456660 MtrunA17_Chr3g0134741 50.000 64 31 1 115 178 230 292 1.66e-11 63.2
Msa1456660 MtrunA17_Chr1g0152651 47.692 65 33 1 114 178 274 337 1.66e-11 63.2
Msa1456660 MtrunA17_Chr7g0262691 38.947 95 52 3 108 197 246 339 1.67e-11 63.5
Msa1456660 MtrunA17_Chr8g0351881 47.692 65 34 0 114 178 316 380 1.87e-11 63.2
Msa1456660 MtrunA17_Chr5g0393721 31.544 149 89 4 38 178 144 287 4.02e-11 62.0
Msa1456660 MtrunA17_Chr7g0270871 45.455 55 29 1 125 179 213 266 7.00e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456660 AT1G59640.1 53.957 278 77 9 1 241 1 264 6.68e-83 248
Msa1456660 AT1G59640.2 48.302 265 83 10 1 225 1 251 4.31e-58 187
Msa1456660 AT5G62610.2 43.841 276 103 8 4 240 18 280 1.77e-56 182
Msa1456660 AT5G62610.1 43.841 276 103 8 4 240 18 280 1.77e-56 182
Msa1456660 AT3G23690.1 58.065 155 51 4 47 187 110 264 7.17e-45 154
Msa1456660 AT1G68920.1 56.757 148 61 3 75 220 264 410 8.45e-44 154
Msa1456660 AT1G68920.4 56.757 148 61 3 75 220 264 410 8.45e-44 154
Msa1456660 AT1G68920.2 56.757 148 61 3 75 220 264 410 8.45e-44 154
Msa1456660 AT1G68920.3 56.757 148 61 3 75 220 263 409 9.14e-44 154
Msa1456660 AT3G07340.2 50.820 183 65 4 13 195 182 339 1.93e-43 152
Msa1456660 AT3G07340.1 51.366 183 64 5 13 195 182 339 2.05e-43 152
Msa1456660 AT5G50915.3 50.617 162 76 3 27 185 47 207 1.40e-42 145
Msa1456660 AT5G50915.4 50.617 162 76 3 27 185 47 207 1.40e-42 145
Msa1456660 AT5G48560.2 85.185 81 12 0 105 185 292 372 2.47e-42 150
Msa1456660 AT5G50915.1 50.932 161 75 3 27 184 47 206 3.71e-42 145
Msa1456660 AT5G50915.2 50.932 161 75 3 27 184 47 206 3.71e-42 145
Msa1456660 AT5G48560.1 85.185 81 12 0 105 185 292 372 4.04e-42 149
Msa1456660 AT4G34530.1 47.727 176 84 2 13 188 79 246 2.73e-40 141
Msa1456660 AT2G18300.2 46.369 179 71 4 37 210 121 279 6.59e-40 140
Msa1456660 AT1G26260.3 70.000 100 26 1 90 185 144 243 7.82e-40 140
Msa1456660 AT1G26260.5 70.000 100 26 1 90 185 173 272 1.40e-39 140
Msa1456660 AT2G18300.3 46.369 179 71 4 37 210 121 279 1.62e-39 140
Msa1456660 AT1G26260.4 70.000 100 26 1 90 185 195 294 2.02e-39 140
Msa1456660 AT1G26260.1 70.000 100 26 1 90 185 195 294 2.02e-39 140
Msa1456660 AT1G26260.6 70.000 100 26 1 90 185 195 294 2.02e-39 140
Msa1456660 AT1G26260.2 70.000 100 26 1 90 185 195 294 2.02e-39 140
Msa1456660 AT1G10120.2 55.385 130 54 1 88 213 180 309 3.61e-39 139
Msa1456660 AT1G10120.1 55.385 130 54 1 88 213 180 309 3.61e-39 139
Msa1456660 AT4G36540.1 60.317 126 44 3 82 207 113 232 7.80e-39 137
Msa1456660 AT2G18300.1 45.810 179 70 4 37 210 121 277 8.11e-39 137
Msa1456660 AT4G36540.2 52.318 151 57 4 82 221 113 259 1.65e-38 136
Msa1456660 AT2G42300.4 73.750 80 21 0 108 187 179 258 1.02e-35 128
Msa1456660 AT3G57800.2 73.810 84 22 0 104 187 194 277 1.70e-35 130
Msa1456660 AT2G42300.1 73.750 80 21 0 108 187 179 258 6.63e-35 127
Msa1456660 AT1G73830.1 55.000 120 54 0 67 186 100 219 7.44e-35 125
Msa1456660 AT1G73830.2 55.000 120 54 0 67 186 100 219 7.50e-35 125
Msa1456660 AT1G18400.2 73.333 75 20 0 112 186 143 217 1.43e-33 122
Msa1456660 AT1G18400.1 73.333 75 20 0 112 186 143 217 2.23e-33 121
Msa1456660 AT1G25330.1 69.231 78 24 0 109 186 99 176 2.23e-33 120
Msa1456660 AT3G57800.1 47.328 131 22 1 104 187 194 324 1.07e-26 107
Msa1456660 AT2G24260.2 60.274 73 28 1 107 179 28 99 2.24e-21 89.7
Msa1456660 AT4G30980.1 49.038 104 44 3 76 179 100 194 6.36e-21 89.7
Msa1456660 AT5G58010.1 66.667 66 21 1 114 179 99 163 1.63e-20 88.6
Msa1456660 AT2G24260.1 60.274 73 28 1 107 179 131 202 1.97e-20 89.0
Msa1456660 AT4G30980.2 49.038 104 44 3 76 179 148 242 2.30e-20 89.0
Msa1456660 AT2G42300.5 80.435 46 9 0 108 153 179 224 5.32e-19 82.8
Msa1456660 AT2G42300.3 80.435 46 9 0 108 153 179 224 5.32e-19 82.8
Msa1456660 AT2G42300.2 80.435 46 9 0 108 153 179 224 6.55e-19 82.8
Msa1456660 AT2G20180.7 50.794 63 30 1 117 179 210 271 2.75e-14 72.0
Msa1456660 AT2G20180.1 50.794 63 30 1 117 179 210 271 2.75e-14 72.0
Msa1456660 AT2G20180.5 50.794 63 30 1 117 179 210 271 2.75e-14 72.0
Msa1456660 AT1G09530.3 48.438 64 32 1 116 179 339 401 3.72e-14 71.6
Msa1456660 AT1G09530.2 48.438 64 32 1 116 179 339 401 3.72e-14 71.6
Msa1456660 AT1G09530.5 48.438 64 32 1 116 179 339 401 3.72e-14 71.6
Msa1456660 AT1G09530.1 48.438 64 32 1 116 179 339 401 3.72e-14 71.6
Msa1456660 AT1G09530.4 48.438 64 32 1 116 179 339 401 3.72e-14 71.6
Msa1456660 AT1G09530.6 48.438 64 32 1 116 179 339 401 3.72e-14 71.6
Msa1456660 AT2G20180.4 50.794 63 30 1 117 179 281 342 4.24e-14 71.6
Msa1456660 AT2G20180.6 50.794 63 30 1 117 179 281 342 4.24e-14 71.6
Msa1456660 AT2G20180.2 50.794 63 30 1 117 179 281 342 4.24e-14 71.6
Msa1456660 AT2G20180.3 50.794 63 30 1 117 179 281 342 4.24e-14 71.6
Msa1456660 AT4G28815.2 45.946 74 38 2 117 189 71 143 1.51e-13 68.2
Msa1456660 AT4G02590.3 41.803 122 60 4 58 179 37 147 3.18e-13 67.4
Msa1456660 AT4G28800.1 50.000 62 30 1 117 178 252 312 3.46e-13 68.9
Msa1456660 AT4G28815.1 45.946 74 38 2 117 189 147 219 4.27e-13 67.8
Msa1456660 AT4G02590.2 41.803 122 60 4 58 179 100 210 7.07e-13 67.4
Msa1456660 AT4G02590.1 41.803 122 60 4 58 179 100 210 7.07e-13 67.4
Msa1456660 AT1G51140.1 51.562 64 31 0 114 177 304 367 1.07e-12 67.0
Msa1456660 AT4G28790.1 30.189 159 99 4 31 179 179 335 1.20e-12 67.0
Msa1456660 AT4G28800.2 45.833 72 37 2 117 187 264 334 1.22e-12 67.0
Msa1456660 AT2G20180.8 50.000 60 29 1 117 176 281 339 1.74e-12 66.2
Msa1456660 AT1G03040.2 59.091 66 26 1 114 179 139 203 1.80e-12 65.9
Msa1456660 AT1G03040.1 59.091 66 26 1 114 179 144 208 2.07e-12 65.9
Msa1456660 AT1G03040.3 59.091 66 26 1 114 179 144 208 2.97e-12 65.5
Msa1456660 AT1G35460.1 40.000 75 45 0 114 188 181 255 5.86e-12 64.3
Msa1456660 AT2G46970.1 37.895 95 53 2 90 179 194 287 6.63e-12 65.1
Msa1456660 AT2G46970.2 37.895 95 53 2 90 179 163 256 6.77e-12 64.7
Msa1456660 AT2G46970.3 37.895 95 53 2 90 179 172 265 6.97e-12 64.7
Msa1456660 AT2G42280.1 46.154 65 35 0 114 178 279 343 9.26e-12 64.3
Msa1456660 AT4G09180.1 43.284 67 38 0 112 178 182 248 1.18e-11 63.2
Msa1456660 AT4G36930.1 33.929 112 70 3 120 229 197 306 1.21e-11 63.9
Msa1456660 AT2G42280.3 48.485 66 32 1 114 177 279 344 1.67e-11 63.5
Msa1456660 AT4G00050.2 47.692 65 33 1 114 178 105 168 2.36e-11 62.8
Msa1456660 AT1G27740.1 50.000 64 31 1 115 178 168 230 2.79e-11 62.4
Msa1456660 AT2G43140.2 46.377 69 37 0 114 182 231 299 3.37e-11 62.4
Msa1456660 AT4G00050.1 47.692 65 33 1 114 178 207 270 4.15e-11 62.4
Msa1456660 AT2G43140.1 46.377 69 37 0 114 182 134 202 4.89e-11 60.8
Msa1456660 AT4G00050.3 47.692 65 33 1 114 178 207 270 5.00e-11 62.4
Msa1456660 AT4G28790.2 29.677 155 97 4 31 175 179 331 5.97e-11 62.0

Find 98 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTGAGTATATGATTTA+TGG 0.135504 tig0037657:+5370 None:intergenic
TCCTATTTGCATTTACTATC+AGG 0.256165 tig0037657:+5417 None:intergenic
ATCAGTAGCTTGACCCCTTC+TGG 0.262138 tig0037657:+7297 None:intergenic
GGGGCTAAACTGCGGTCTTC+TGG 0.273092 tig0037657:+8422 None:intergenic
TCAGATTTCGAATCTTTCTA+TGG 0.279173 tig0037657:-8478 Msa1456660:exon
ACATGGATGTAGTCTTTCTT+AGG 0.315186 tig0037657:+7325 None:intergenic
TGAAAGGATGAAAACTCTTC+AGG 0.347449 tig0037657:-6442 Msa1456660:CDS
TCTATACCACAGGGTCGTTT+TGG 0.349836 tig0037657:-5315 Msa1456660:three_prime_UTR
GACCCTGTGGTATAGAAAAC+TGG 0.360558 tig0037657:+5322 None:intergenic
ACTGGAATCAAGTGTCCATT+TGG 0.368001 tig0037657:+5340 None:intergenic
ACCTTGAGTATATGATTTAT+GGG 0.371471 tig0037657:+5371 None:intergenic
TGTTTGTTCTGAATACATAA+TGG 0.384620 tig0037657:-5170 Msa1456660:three_prime_UTR
ATACTCTTTCTGCAAGACTA+TGG 0.386857 tig0037657:+7272 None:intergenic
CGTATCATCTCCCGGAGATC+TGG 0.389824 tig0037657:-8449 Msa1456660:CDS
AAAGGAGAAGAAAACAGTTC+AGG 0.396640 tig0037657:-7382 Msa1456660:CDS
TTACATAAGATCTAGCTTAG+TGG 0.409585 tig0037657:-5227 Msa1456660:three_prime_UTR
ACCTGATAGTAAATGCAAAT+AGG 0.412337 tig0037657:-5418 Msa1456660:three_prime_UTR
CATTGGAAAATCTCCGAAGC+TGG 0.412609 tig0037657:+8401 None:intergenic
CTTCTGGACTGCCAGATCTC+CGG 0.419175 tig0037657:+8438 None:intergenic
ACACTGATGGACAGGCTAGA+AGG 0.430987 tig0037657:-6476 Msa1456660:intron
TTCTGGACTGCCAGATCTCC+GGG 0.448211 tig0037657:+8439 None:intergenic
ACTCTTCAGGATTTAGTCCC+TGG 0.449489 tig0037657:-6429 Msa1456660:CDS
CTACTTGGTGGTGAAGTGAT+TGG 0.461423 tig0037657:+6128 None:intergenic
GTGGTAGTGGTGATGGAAAA+AGG 0.464313 tig0037657:-7426 Msa1456660:CDS
GTGGGCAATTACCACGTTGT+TGG 0.465601 tig0037657:+8263 None:intergenic
ATGTAGTCTTTCTTAGGATG+TGG 0.471510 tig0037657:+7331 None:intergenic
TCAAGGTGTTGAAGTCCAAA+TGG 0.481629 tig0037657:-5355 Msa1456660:three_prime_UTR
CCGCAGTTTAGCCCCAGCTT+CGG 0.487641 tig0037657:-8414 Msa1456660:CDS
AACCATACCTTGTTGCAACC+AGG 0.489186 tig0037657:+6411 None:intergenic
CGTTTCTACCGGCAATGGTG+GGG 0.491960 tig0037657:-8298 Msa1456660:CDS
TGCGTTTCTACCGGCAATGG+TGG 0.495991 tig0037657:-8300 Msa1456660:CDS
CAAGTTGTAGAGAGAGTGAA+TGG 0.498361 tig0037657:+8574 None:intergenic
TTACATATGCAGCTGGGAGG+TGG 0.500933 tig0037657:-5476 Msa1456660:CDS
TCAGTTAAATGCGTTTCTAC+CGG 0.501738 tig0037657:-8309 Msa1456660:CDS
AGATAATTCACACCTCTACT+TGG 0.503743 tig0037657:+6113 None:intergenic
AGAGTGGTTACATATGCAGC+TGG 0.504135 tig0037657:-5483 Msa1456660:CDS
ATTGGAAAATCTCCGAAGCT+GGG 0.505786 tig0037657:+8402 None:intergenic
ACAGTGAATGAATAGTGATA+TGG 0.506101 tig0037657:+8598 None:intergenic
CACTGATGGACAGGCTAGAA+GGG 0.507526 tig0037657:-6475 Msa1456660:intron
GATGATGGTGGTAGTGGTGA+TGG 0.509548 tig0037657:-7433 Msa1456660:CDS
GACCCCTTCTGGCTCGAACA+TGG 0.511902 tig0037657:+7308 None:intergenic
GGGTTTGTGCAGAATGATGA+TGG 0.512242 tig0037657:-7448 Msa1456660:intron
TCAGGTTATTGGAAAAGCAT+TGG 0.514448 tig0037657:-6176 Msa1456660:intron
TATGATCATTGTTAGTGTGC+TGG 0.516331 tig0037657:-5262 Msa1456660:intron
GATTTCAATTTATTCAACAT+AGG 0.519995 tig0037657:+5089 None:intergenic
AACAGCGAGGGAGGGAAGAA+TGG 0.520840 tig0037657:+8526 None:intergenic
GTTTCTACCGGCAATGGTGG+GGG 0.521306 tig0037657:-8297 Msa1456660:CDS
ACCAAACAATTAAAATCGCA+TGG 0.523285 tig0037657:+5067 None:intergenic
GCGTTTCTACCGGCAATGGT+GGG 0.526543 tig0037657:-8299 Msa1456660:CDS
AAGACATCAGGGAACAGTAA+AGG 0.528363 tig0037657:-7400 Msa1456660:CDS
CAGAATGATGATGGTGGTAG+TGG 0.528680 tig0037657:-7439 Msa1456660:CDS
CATCCATGTTCGAGCCAGAA+GGG 0.533625 tig0037657:-7311 Msa1456660:CDS
ATGATCATTGTTAGTGTGCT+GGG 0.538146 tig0037657:-5261 Msa1456660:intron
GGAGGAAACGCCTCAATACC+AGG 0.545073 tig0037657:+5968 None:intergenic
AAATGCGTTTCTACCGGCAA+TGG 0.547123 tig0037657:-8303 Msa1456660:CDS
AATAGTGATATGGTTGGAGT+TGG 0.550623 tig0037657:+8608 None:intergenic
AAGACCAACACCTGGTATTG+AGG 0.552267 tig0037657:-5978 Msa1456660:CDS
TATAATTGATTATCTCATCA+AGG 0.561459 tig0037657:+6152 None:intergenic
ATTATAGAGTTTAACAGCGA+GGG 0.563815 tig0037657:+8514 None:intergenic
ATCATTGTTGTTGTAAAAGA+CGG 0.567147 tig0037657:-5194 Msa1456660:three_prime_UTR
GGATCCAGCGTATCATCTCC+CGG 0.570528 tig0037657:-8457 Msa1456660:CDS
ACCATACCTTGTTGCAACCA+GGG 0.571972 tig0037657:+6412 None:intergenic
GAAGGGAAAAGATTAGTGAA+AGG 0.577890 tig0037657:-6458 Msa1456660:CDS
TAATTCACACCTCTACTTGG+TGG 0.582744 tig0037657:+6116 None:intergenic
ACATCCATGTTCGAGCCAGA+AGG 0.584719 tig0037657:-7312 Msa1456660:CDS
TCAGCACTGAGATCTTCCAT+TGG 0.586033 tig0037657:+8384 None:intergenic
TCCCATAAATCATATACTCA+AGG 0.586272 tig0037657:-5372 Msa1456660:three_prime_UTR
CCACGTTGTTGGAATTGCCA+CGG 0.588109 tig0037657:+8274 None:intergenic
ATATGGTTGGAGTTGGACAC+TGG 0.593652 tig0037657:+8615 None:intergenic
ATAGAGTTTAACAGCGAGGG+AGG 0.594812 tig0037657:+8517 None:intergenic
CTTGTAGCTTGTGAAGCGAA+TGG 0.606152 tig0037657:+5530 None:intergenic
AACGCCTCAATACCAGGTGT+TGG 0.606994 tig0037657:+5974 None:intergenic
ATCCATGTTCGAGCCAGAAG+GGG 0.611344 tig0037657:-7310 Msa1456660:CDS
TAGAGTTTAACAGCGAGGGA+GGG 0.612328 tig0037657:+8518 None:intergenic
AGCTGCATATGTAACCACTC+TGG 0.617342 tig0037657:+5485 None:intergenic
GAAAAGTAGAATAGAGACGA+AGG 0.620221 tig0037657:-8337 Msa1456660:CDS
TTGGAAAATCTCCGAAGCTG+GGG 0.627605 tig0037657:+8403 None:intergenic
TTTAGTCCCTGGTTGCAACA+AGG 0.628236 tig0037657:-6418 Msa1456660:intron
ATCTCCGGGAGATGATACGC+TGG 0.632287 tig0037657:+8453 None:intergenic
ATCACTTCACCACCAAGTAG+AGG 0.638957 tig0037657:-6125 Msa1456660:intron
GAGTGGTTACATATGCAGCT+GGG 0.656275 tig0037657:-5482 Msa1456660:CDS
TATTATAGAGTTTAACAGCG+AGG 0.665362 tig0037657:+8513 None:intergenic
ACCACTCTGGTGATGCACCT+CGG 0.668855 tig0037657:+5498 None:intergenic
TTGTATGGGAGGGAGTCCCA+AGG 0.669417 tig0037657:-8717 None:intergenic
TGAATGAATAGTGATATGGT+TGG 0.671981 tig0037657:+8602 None:intergenic
GCTACAAGAGAGTACAGCCG+AGG 0.682744 tig0037657:-5515 Msa1456660:CDS
TACAAACCAAAACGACCCTG+TGG 0.687298 tig0037657:+5309 None:intergenic
AGCTTGTGAAGCGAATGGTA+TGG 0.687729 tig0037657:+5535 None:intergenic
AAACAGTTCAGGAAAGCATG+CGG 0.691753 tig0037657:-7371 Msa1456660:CDS
CCGAAGCTGGGGCTAAACTG+CGG 0.692738 tig0037657:+8414 None:intergenic
TTGTGAAGCGAATGGTATGG+TGG 0.693506 tig0037657:+5538 None:intergenic
GTAAATTCAAGACCAACACC+TGG 0.698508 tig0037657:-5986 Msa1456660:CDS
GCCGAGGTGCATCACCAGAG+TGG 0.699435 tig0037657:-5499 Msa1456660:CDS
TGGTTACATATGCAGCTGGG+AGG 0.711832 tig0037657:-5479 Msa1456660:CDS
CACTGGACAGTGTAGCGTGG+TGG 0.717387 tig0037657:+8632 None:intergenic
CCGTGGCAATTCCAACAACG+TGG 0.736074 tig0037657:-8274 Msa1456660:intron
GGACACTGGACAGTGTAGCG+TGG 0.741944 tig0037657:+8629 None:intergenic
TTTGTGCAGAATGATGATGG+TGG 0.743796 tig0037657:-7445 Msa1456660:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATTTAGAAATATATAT+TGG - tig0037657:5614-5633 Msa1456660:intron 10.0%
!! TGAATTTAGAAATATATATT+GGG - tig0037657:5615-5634 Msa1456660:intron 10.0%
!!! GCTTTATTTTATTTTTATTA+TGG - tig0037657:6047-6066 Msa1456660:intron 10.0%
!! AATAAAACCTATATAAATTT+AGG + tig0037657:6855-6874 None:intergenic 10.0%
!!! ACATTAAATTTTTAGAAAAA+AGG + tig0037657:7705-7724 None:intergenic 10.0%
!! TTATGTTATCTTAATTATGA+AGG - tig0037657:5573-5592 Msa1456660:intron 15.0%
!! ATGAAGGAAAAAATATATAT+TGG - tig0037657:5589-5608 Msa1456660:intron 15.0%
!! TGAAGGAAAAAATATATATT+GGG - tig0037657:5590-5609 Msa1456660:intron 15.0%
!! AATTCCAACAAAAAAAAAAA+TGG + tig0037657:6278-6297 None:intergenic 15.0%
!! AACAATTCCTAAATTTATAT+AGG - tig0037657:6845-6864 Msa1456660:intron 15.0%
!!! TATCTGAAAATTTTCTAAAA+AGG - tig0037657:7170-7189 Msa1456660:intron 15.0%
!! AAACATAATAAATCTATTTG+AGG - tig0037657:8039-8058 Msa1456660:intron 15.0%
!!! TTTAGTTCTACTAGAAAAAT+TGG - tig0037657:5644-5663 Msa1456660:intron 20.0%
!!! TATTTGAGTGTTTTTTTTAC+TGG - tig0037657:5750-5769 Msa1456660:intron 20.0%
!!! AATAAAGCTATTTTCATGTA+AGG + tig0037657:6035-6054 None:intergenic 20.0%
!!! TCACAAAGTGTATTTTTATA+TGG - tig0037657:6549-6568 Msa1456660:intron 20.0%
!!! AGTAAATTGTGTTTCTATTT+GGG + tig0037657:6792-6811 None:intergenic 20.0%
!!! TAGTAAATTGTGTTTCTATT+TGG + tig0037657:6793-6812 None:intergenic 20.0%
!!! TTAATGGTTGTTTTAGTTTT+AGG - tig0037657:7049-7068 Msa1456660:intron 20.0%
!!! GATTTGTTTTTAATGTATTC+AGG - tig0037657:7586-7605 Msa1456660:intron 20.0%
!!! TTTTAATGTATTCAGGTTAT+TGG - tig0037657:7593-7612 Msa1456660:intron 20.0%
!! TATAATTGATTATCTCATCA+AGG + tig0037657:7631-7650 None:intergenic 20.0%
!!! TGTTAAATACTTACAGTTTT+AGG + tig0037657:7836-7855 None:intergenic 20.0%
!! CATTCAACGTAAAAAAAATT+AGG + tig0037657:7863-7882 None:intergenic 20.0%
!! TTATTCTATATAATGTCCTT+TGG - tig0037657:7914-7933 Msa1456660:intron 20.0%
!!! TTTAGTCCTCAAATTTTTAA+CGG + tig0037657:8069-8088 None:intergenic 20.0%
!! GATTTCAATTTATTCAACAT+AGG + tig0037657:8694-8713 None:intergenic 20.0%
! AAAAGATAGCATAATTGAGT+GGG + tig0037657:5538-5557 None:intergenic 25.0%
! AAAAAGATAGCATAATTGAG+TGG + tig0037657:5539-5558 None:intergenic 25.0%
! AGTATTAACCAAGTAGTTAA+AGG - tig0037657:5692-5711 Msa1456660:intron 25.0%
!!! GAGTTTTTAAAACAACTGTT+TGG - tig0037657:5800-5819 Msa1456660:intron 25.0%
!!! GTTGTTTTTGTAAGCTATAT+TGG - tig0037657:5906-5925 Msa1456660:intron 25.0%
! TGCAATTTCGTATTACAATA+TGG - tig0037657:6236-6255 Msa1456660:intron 25.0%
! CCAACAAAAAAAAAAATGGT+AGG + tig0037657:6274-6293 None:intergenic 25.0%
!!! CCTACCATTTTTTTTTTTGT+TGG - tig0037657:6271-6290 Msa1456660:intron 25.0%
!!! TGTTACTTTTTTTGACAACT+TGG - tig0037657:6646-6665 Msa1456660:intron 25.0%
!! TTCTTATATTTAAGACCAGA+GGG + tig0037657:6733-6752 None:intergenic 25.0%
!!! TTTAGGAATTGTTTTTGAGA+AGG + tig0037657:6838-6857 None:intergenic 25.0%
! AAATGAAACTGTTACGTAAA+TGG - tig0037657:7204-7223 Msa1456660:intron 25.0%
! AATGAAACTGTTACGTAAAT+GGG - tig0037657:7205-7224 Msa1456660:intron 25.0%
! TAAAAACAAATCAAGCCATA+AGG + tig0037657:7578-7597 None:intergenic 25.0%
!!! TAAATACTTACAGTTTTAGG+AGG + tig0037657:7833-7852 None:intergenic 25.0%
!! ATGCTATATATTCTAGAAGT+TGG - tig0037657:7880-7899 Msa1456660:intron 25.0%
!!! TAAACTCTTTAATTTGGACT+AGG + tig0037657:8126-8145 None:intergenic 25.0%
! GCACTTTAAACTCTTTAATT+TGG + tig0037657:8132-8151 None:intergenic 25.0%
! GAAGATAGATTTAGCTAAAA+AGG + tig0037657:8168-8187 None:intergenic 25.0%
! ACCTTGAGTATATGATTTAT+GGG + tig0037657:8412-8431 None:intergenic 25.0%
!! ATCATTGTTGTTGTAAAAGA+CGG - tig0037657:8586-8605 Msa1456660:five_prime_UTR 25.0%
!! TGTTTGTTCTGAATACATAA+TGG - tig0037657:8610-8629 Msa1456660:five_prime_UTR 25.0%
TGAATGAATAGTGATATGGT+TGG + tig0037657:5181-5200 None:intergenic 30.0%
ACAGTGAATGAATAGTGATA+TGG + tig0037657:5185-5204 None:intergenic 30.0%
ATTATAGAGTTTAACAGCGA+GGG + tig0037657:5269-5288 None:intergenic 30.0%
TATTATAGAGTTTAACAGCG+AGG + tig0037657:5270-5289 None:intergenic 30.0%
TCAGATTTCGAATCTTTCTA+TGG - tig0037657:5302-5321 Msa1456660:three_prime_UTR 30.0%
TAACCAAGTAGTTAAAGGTT+AGG - tig0037657:5697-5716 Msa1456660:intron 30.0%
!!! GCAACTTGTTTTTGTATATG+TGG - tig0037657:5880-5899 Msa1456660:intron 30.0%
! AATTGTCACAAGTTGTTTCA+TGG - tig0037657:5941-5960 Msa1456660:intron 30.0%
GATTAAGATGTTTATCCAAG+TGG - tig0037657:6108-6127 Msa1456660:intron 30.0%
TACTCATTAAAACTTCCACT+TGG + tig0037657:6126-6145 None:intergenic 30.0%
! TGGTAATTTAGAGTTTGACT+AGG + tig0037657:6171-6190 None:intergenic 30.0%
AGTCAAACTCTAAATTACCA+AGG - tig0037657:6171-6190 Msa1456660:CDS 30.0%
AAACTCTAAATTACCAAGGT+CGG - tig0037657:6175-6194 Msa1456660:CDS 30.0%
TCGTATTACAATATGGAGAA+TGG - tig0037657:6243-6262 Msa1456660:intron 30.0%
! TGGAATTCAAGCATTGTTAT+TGG - tig0037657:6291-6310 Msa1456660:intron 30.0%
CTTTGTGAGAATTAGAAAGT+AGG + tig0037657:6537-6556 None:intergenic 30.0%
TTAGATAATGTTGATCCTGA+AGG - tig0037657:6589-6608 Msa1456660:intron 30.0%
! CTTCTTATATTTAAGACCAG+AGG + tig0037657:6734-6753 None:intergenic 30.0%
CAAGTTTCATGTTCTTAACT+GGG - tig0037657:7094-7113 Msa1456660:intron 30.0%
!! GGGAGGAAATATTGATTTAT+TGG - tig0037657:7225-7244 Msa1456660:intron 30.0%
GCAATCATATTCAGTGTTAT+TGG - tig0037657:7255-7274 Msa1456660:intron 30.0%
TGAATATTGATGAACACTGA+TGG - tig0037657:7291-7310 Msa1456660:CDS 30.0%
TGAGCTGAAAATATTCCTTA+TGG - tig0037657:7560-7579 Msa1456660:intron 30.0%
AAAAAAGCTTCTAACCTAAC+TGG + tig0037657:7978-7997 None:intergenic 30.0%
! GGTTTTCCGTTAAAAATTTG+AGG - tig0037657:8060-8079 Msa1456660:intron 30.0%
TTGGTCACTCAATGATTATT+GGG + tig0037657:8096-8115 None:intergenic 30.0%
TTTGGTCACTCAATGATTAT+TGG + tig0037657:8097-8116 None:intergenic 30.0%
ACCTGATAGTAAATGCAAAT+AGG - tig0037657:8362-8381 Msa1456660:CDS 30.0%
TCCTATTTGCATTTACTATC+AGG + tig0037657:8366-8385 None:intergenic 30.0%
TCCCATAAATCATATACTCA+AGG - tig0037657:8408-8427 Msa1456660:CDS 30.0%
CACCTTGAGTATATGATTTA+TGG + tig0037657:8413-8432 None:intergenic 30.0%
! TTACATAAGATCTAGCTTAG+TGG - tig0037657:8553-8572 Msa1456660:five_prime_UTR 30.0%
! TTTTCAGACACATCTACCTT+GGG + tig0037657:5082-5101 None:intergenic 35.0%
AATAGTGATATGGTTGGAGT+TGG + tig0037657:5175-5194 None:intergenic 35.0%
!! TCGTCTCTATTCTACTTTTC+CGG + tig0037657:5443-5462 None:intergenic 35.0%
GAAAAGTAGAATAGAGACGA+AGG - tig0037657:5443-5462 Msa1456660:exon 35.0%
TCAGTTAAATGCGTTTCTAC+CGG - tig0037657:5471-5490 Msa1456660:CDS 35.0%
GAACCTAACCTTTAACTACT+TGG + tig0037657:5703-5722 None:intergenic 35.0%
AAGGTTAGGTTCAACTCTTA+AGG - tig0037657:5711-5730 Msa1456660:intron 35.0%
! TATCACATAGAGAGCTTGTT+TGG + tig0037657:5990-6009 None:intergenic 35.0%
TTTCAAAAGTAAACCGACCT+TGG + tig0037657:6191-6210 None:intergenic 35.0%
! AAGCATTGTTATTGGAGTAG+AGG - tig0037657:6299-6318 Msa1456660:intron 35.0%
GAAAAAGGAGTAAGACATCA+GGG - tig0037657:6369-6388 Msa1456660:intron 35.0%
AAAGGAGAAGAAAACAGTTC+AGG - tig0037657:6398-6417 Msa1456660:intron 35.0%
ATGTAGTCTTTCTTAGGATG+TGG + tig0037657:6452-6471 None:intergenic 35.0%
ACATGGATGTAGTCTTTCTT+AGG + tig0037657:6458-6477 None:intergenic 35.0%
ATACTCTTTCTGCAAGACTA+TGG + tig0037657:6511-6530 None:intergenic 35.0%
TTTGACAACTTGGAATCAGT+AGG - tig0037657:6656-6675 Msa1456660:intron 35.0%
! TATATTTAAGACCAGAGGGA+GGG + tig0037657:6729-6748 None:intergenic 35.0%
! TTATATTTAAGACCAGAGGG+AGG + tig0037657:6730-6749 None:intergenic 35.0%
CGGTCTCGATTATAAACAAA+AGG + tig0037657:7015-7034 None:intergenic 35.0%
TGTTTATAATCGAGACCGAA+TGG - tig0037657:7017-7036 Msa1456660:intron 35.0%
CATTAAGCAAAACCACCATT+CGG + tig0037657:7035-7054 None:intergenic 35.0%
GCAAGTTTCATGTTCTTAAC+TGG - tig0037657:7093-7112 Msa1456660:intron 35.0%
CTGGGATAATTGTAATACTG+AGG - tig0037657:7112-7131 Msa1456660:intron 35.0%
GAAGGGAAAAGATTAGTGAA+AGG - tig0037657:7322-7341 Msa1456660:CDS 35.0%
TGAAAGGATGAAAACTCTTC+AGG - tig0037657:7338-7357 Msa1456660:CDS 35.0%
! TCAGGTTATTGGAAAAGCAT+TGG - tig0037657:7604-7623 Msa1456660:intron 35.0%
AGATAATTCACACCTCTACT+TGG + tig0037657:7670-7689 None:intergenic 35.0%
AGTTATACTCATCTCACCAA+AGG + tig0037657:7933-7952 None:intergenic 35.0%
GGTATCCTTGCGATAAAAAA+GGG + tig0037657:7957-7976 None:intergenic 35.0%
TGGTATCCTTGCGATAAAAA+AGG + tig0037657:7958-7977 None:intergenic 35.0%
!! ATTTGGACTAGGTCTGATTT+TGG + tig0037657:8115-8134 None:intergenic 35.0%
! CATATGTTTGCTGATCAAAC+TGG + tig0037657:8216-8235 None:intergenic 35.0%
! ATTCCAGTTTTCTATACCAC+AGG - tig0037657:8455-8474 Msa1456660:CDS 35.0%
! TTCCAGTTTTCTATACCACA+GGG - tig0037657:8456-8475 Msa1456660:CDS 35.0%
TATGATCATTGTTAGTGTGC+TGG - tig0037657:8518-8537 Msa1456660:five_prime_UTR 35.0%
ATGATCATTGTTAGTGTGCT+GGG - tig0037657:8519-8538 Msa1456660:five_prime_UTR 35.0%
! GTTTTCAGACACATCTACCT+TGG + tig0037657:5083-5102 None:intergenic 40.0%
CAAGTTGTAGAGAGAGTGAA+TGG + tig0037657:5209-5228 None:intergenic 40.0%
ATTGGAAAATCTCCGAAGCT+GGG + tig0037657:5381-5400 None:intergenic 40.0%
GAACAAAAACCACTGAACCA+TGG - tig0037657:5417-5436 Msa1456660:three_prime_UTR 40.0%
GTTCAACTCTTAAGGTGTGT+TGG - tig0037657:5719-5738 Msa1456660:intron 40.0%
TGGAGTAGAGGTAGTGAATT+TGG - tig0037657:6311-6330 Msa1456660:intron 40.0%
GGAGTAGAGGTAGTGAATTT+GGG - tig0037657:6312-6331 Msa1456660:intron 40.0%
!! TTTGTGCAGAATGATGATGG+TGG - tig0037657:6335-6354 Msa1456660:intron 40.0%
GGAAAAAGGAGTAAGACATC+AGG - tig0037657:6368-6387 Msa1456660:intron 40.0%
AAGACATCAGGGAACAGTAA+AGG - tig0037657:6380-6399 Msa1456660:intron 40.0%
! AAACAGTTCAGGAAAGCATG+CGG - tig0037657:6409-6428 Msa1456660:intron 40.0%
TCAAGCAAGCAATATCCTTC+AGG + tig0037657:6607-6626 None:intergenic 40.0%
TTATAATCGAGACCGAATGG+TGG - tig0037657:7020-7039 Msa1456660:intron 40.0%
!! CGAATGGTGGTTTTGCTTAA+TGG - tig0037657:7033-7052 Msa1456660:intron 40.0%
GAAACTGTTACGTAAATGGG+AGG - tig0037657:7208-7227 Msa1456660:intron 40.0%
! ATTGATGAACACTGATGGAC+AGG - tig0037657:7296-7315 Msa1456660:CDS 40.0%
TAATTCACACCTCTACTTGG+TGG + tig0037657:7667-7686 None:intergenic 40.0%
GTAAATTCAAGACCAACACC+TGG - tig0037657:7794-7813 Msa1456660:intron 40.0%
!!! AACTGCCCTTTTTTATCGCA+AGG - tig0037657:7949-7968 Msa1456660:intron 40.0%
TTATCGCAAGGATACCAGTT+AGG - tig0037657:7961-7980 Msa1456660:intron 40.0%
GATTAGTAGATAGTAGCAGC+AGG - tig0037657:8012-8031 Msa1456660:intron 40.0%
!! TCAAGGTGTTGAAGTCCAAA+TGG - tig0037657:8425-8444 Msa1456660:CDS 40.0%
ACTGGAATCAAGTGTCCATT+TGG + tig0037657:8443-8462 None:intergenic 40.0%
ATATGGTTGGAGTTGGACAC+TGG + tig0037657:5168-5187 None:intergenic 45.0%
TAGAGTTTAACAGCGAGGGA+GGG + tig0037657:5265-5284 None:intergenic 45.0%
ATAGAGTTTAACAGCGAGGG+AGG + tig0037657:5266-5285 None:intergenic 45.0%
TTGGAAAATCTCCGAAGCTG+GGG + tig0037657:5380-5399 None:intergenic 45.0%
CATTGGAAAATCTCCGAAGC+TGG + tig0037657:5382-5401 None:intergenic 45.0%
! CAGCTTCGGAGATTTTCCAA+TGG - tig0037657:5380-5399 Msa1456660:three_prime_UTR 45.0%
! TCAGCACTGAGATCTTCCAT+TGG + tig0037657:5399-5418 None:intergenic 45.0%
AAAAACCACTGAACCATGGC+CGG - tig0037657:5421-5440 Msa1456660:three_prime_UTR 45.0%
!! CTATTCTACTTTTCCGGCCA+TGG + tig0037657:5437-5456 None:intergenic 45.0%
AAATGCGTTTCTACCGGCAA+TGG - tig0037657:5477-5496 Msa1456660:CDS 45.0%
GGGTTTGTGCAGAATGATGA+TGG - tig0037657:6332-6351 Msa1456660:intron 45.0%
!! CAGAATGATGATGGTGGTAG+TGG - tig0037657:6341-6360 Msa1456660:intron 45.0%
!! GTGGTAGTGGTGATGGAAAA+AGG - tig0037657:6354-6373 Msa1456660:intron 45.0%
! GAGGGAGGGAGTATAGAATT+TGG + tig0037657:6715-6734 None:intergenic 45.0%
AATTCTATACTCCCTCCCTC+TGG - tig0037657:6715-6734 Msa1456660:intron 45.0%
ACTCTTCAGGATTTAGTCCC+TGG - tig0037657:7351-7370 Msa1456660:CDS 45.0%
TTTAGTCCCTGGTTGCAACA+AGG - tig0037657:7362-7381 Msa1456660:CDS 45.0%
ACCATACCTTGTTGCAACCA+GGG + tig0037657:7371-7390 None:intergenic 45.0%
AACCATACCTTGTTGCAACC+AGG + tig0037657:7372-7391 None:intergenic 45.0%
GAGCAGAGACAAAACTGAAC+AGG + tig0037657:7404-7423 None:intergenic 45.0%
!! CTACTTGGTGGTGAAGTGAT+TGG + tig0037657:7655-7674 None:intergenic 45.0%
ATCACTTCACCACCAAGTAG+AGG - tig0037657:7655-7674 Msa1456660:intron 45.0%
AAGACCAACACCTGGTATTG+AGG - tig0037657:7802-7821 Msa1456660:intron 45.0%
AGTAGATAGTAGCAGCAGGT+AGG - tig0037657:8016-8035 Msa1456660:intron 45.0%
TTGTGAAGCGAATGGTATGG+TGG + tig0037657:8245-8264 None:intergenic 45.0%
AGCTTGTGAAGCGAATGGTA+TGG + tig0037657:8248-8267 None:intergenic 45.0%
! CTTGTAGCTTGTGAAGCGAA+TGG + tig0037657:8253-8272 None:intergenic 45.0%
AGCTGCATATGTAACCACTC+TGG + tig0037657:8298-8317 None:intergenic 45.0%
AGAGTGGTTACATATGCAGC+TGG - tig0037657:8297-8316 Msa1456660:CDS 45.0%
GAGTGGTTACATATGCAGCT+GGG - tig0037657:8298-8317 Msa1456660:CDS 45.0%
GACCCTGTGGTATAGAAAAC+TGG + tig0037657:8461-8480 None:intergenic 45.0%
! TCTATACCACAGGGTCGTTT+TGG - tig0037657:8465-8484 Msa1456660:exon 45.0%
TACAAACCAAAACGACCCTG+TGG + tig0037657:8474-8493 None:intergenic 45.0%
CCACGTTGTTGGAATTGCCA+CGG + tig0037657:5509-5528 None:intergenic 50.0%
GTGGGCAATTACCACGTTGT+TGG + tig0037657:5520-5539 None:intergenic 50.0%
!! GATGATGGTGGTAGTGGTGA+TGG - tig0037657:6347-6366 Msa1456660:intron 50.0%
ACATCCATGTTCGAGCCAGA+AGG - tig0037657:6468-6487 Msa1456660:intron 50.0%
CATCCATGTTCGAGCCAGAA+GGG - tig0037657:6469-6488 Msa1456660:intron 50.0%
ATCCATGTTCGAGCCAGAAG+GGG - tig0037657:6470-6489 Msa1456660:intron 50.0%
ATCAGTAGCTTGACCCCTTC+TGG + tig0037657:6486-6505 None:intergenic 50.0%
! ACACTGATGGACAGGCTAGA+AGG - tig0037657:7304-7323 Msa1456660:CDS 50.0%
! CACTGATGGACAGGCTAGAA+GGG - tig0037657:7305-7324 Msa1456660:CDS 50.0%
TCCCTGGTTGCAACAAGGTA+TGG - tig0037657:7367-7386 Msa1456660:CDS 50.0%
AACGCCTCAATACCAGGTGT+TGG + tig0037657:7809-7828 None:intergenic 50.0%
TGGTTACATATGCAGCTGGG+AGG - tig0037657:8301-8320 Msa1456660:CDS 50.0%
TTACATATGCAGCTGGGAGG+TGG - tig0037657:8304-8323 Msa1456660:CDS 50.0%
AACAGCGAGGGAGGGAAGAA+TGG + tig0037657:5257-5276 None:intergenic 55.0%
GGATCCAGCGTATCATCTCC+CGG - tig0037657:5323-5342 Msa1456660:three_prime_UTR 55.0%
ATCTCCGGGAGATGATACGC+TGG + tig0037657:5330-5349 None:intergenic 55.0%
CGTATCATCTCCCGGAGATC+TGG - tig0037657:5331-5350 Msa1456660:three_prime_UTR 55.0%
TTCTGGACTGCCAGATCTCC+GGG + tig0037657:5344-5363 None:intergenic 55.0%
CTTCTGGACTGCCAGATCTC+CGG + tig0037657:5345-5364 None:intergenic 55.0%
! CTTTTCCGGCCATGGTTCAG+TGG + tig0037657:5429-5448 None:intergenic 55.0%
TGCGTTTCTACCGGCAATGG+TGG - tig0037657:5480-5499 Msa1456660:CDS 55.0%
GCGTTTCTACCGGCAATGGT+GGG - tig0037657:5481-5500 Msa1456660:CDS 55.0%
CGTTTCTACCGGCAATGGTG+GGG - tig0037657:5482-5501 Msa1456660:CDS 55.0%
GTTTCTACCGGCAATGGTGG+GGG - tig0037657:5483-5502 Msa1456660:CDS 55.0%
CCGTGGCAATTCCAACAACG+TGG - tig0037657:5506-5525 Msa1456660:CDS 55.0%
! CTGAGGCTGACACACTGACT+CGG - tig0037657:7129-7148 Msa1456660:intron 55.0%
!! CTGACACACTGACTCGGCTA+TGG - tig0037657:7135-7154 Msa1456660:intron 55.0%
GGAGGAAACGCCTCAATACC+AGG + tig0037657:7815-7834 None:intergenic 55.0%
! GCTACAAGAGAGTACAGCCG+AGG - tig0037657:8265-8284 Msa1456660:intron 55.0%
! ACCACTCTGGTGATGCACCT+CGG + tig0037657:8285-8304 None:intergenic 55.0%
CACTGGACAGTGTAGCGTGG+TGG + tig0037657:5151-5170 None:intergenic 60.0%
GGACACTGGACAGTGTAGCG+TGG + tig0037657:5154-5173 None:intergenic 60.0%
GGGGCTAAACTGCGGTCTTC+TGG + tig0037657:5361-5380 None:intergenic 60.0%
CCGAAGCTGGGGCTAAACTG+CGG + tig0037657:5369-5388 None:intergenic 60.0%
CCGCAGTTTAGCCCCAGCTT+CGG - tig0037657:5366-5385 Msa1456660:three_prime_UTR 60.0%
!! GACCCCTTCTGGCTCGAACA+TGG + tig0037657:6475-6494 None:intergenic 60.0%
GCCGAGGTGCATCACCAGAG+TGG - tig0037657:8281-8300 Msa1456660:CDS 65.0%
! ACCGGCAATGGTGGGGGCCG+TGG - tig0037657:5489-5508 Msa1456660:CDS 75.0%
GCCACGGCCCCCACCATTGC+CGG + tig0037657:5493-5512 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0037657 gene 5073 8729 5073 ID=Msa1456660;Name=Msa1456660
tig0037657 mRNA 5073 8729 5073 ID=Msa1456660-mRNA-1;Parent=Msa1456660;Name=Msa1456660-mRNA-1;_AED=0.07;_eAED=0.07;_QI=248|1|1|1|0.83|0.71|7|364|241
tig0037657 exon 5073 5269 5073 ID=Msa1456660-mRNA-1:exon:20850;Parent=Msa1456660-mRNA-1
tig0037657 exon 5290 5585 5290 ID=Msa1456660-mRNA-1:exon:20849;Parent=Msa1456660-mRNA-1
tig0037657 exon 5961 6032 5961 ID=Msa1456660-mRNA-1:exon:20848;Parent=Msa1456660-mRNA-1
tig0037657 exon 6126 6194 6126 ID=Msa1456660-mRNA-1:exon:20847;Parent=Msa1456660-mRNA-1
tig0037657 exon 6419 6484 6419 ID=Msa1456660-mRNA-1:exon:20846;Parent=Msa1456660-mRNA-1
tig0037657 exon 7276 7458 7276 ID=Msa1456660-mRNA-1:exon:20845;Parent=Msa1456660-mRNA-1
tig0037657 exon 8275 8729 8275 ID=Msa1456660-mRNA-1:exon:20844;Parent=Msa1456660-mRNA-1
tig0037657 five_prime_UTR 8482 8729 8482 ID=Msa1456660-mRNA-1:five_prime_utr;Parent=Msa1456660-mRNA-1
tig0037657 CDS 8275 8481 8275 ID=Msa1456660-mRNA-1:cds;Parent=Msa1456660-mRNA-1
tig0037657 CDS 7276 7458 7276 ID=Msa1456660-mRNA-1:cds;Parent=Msa1456660-mRNA-1
tig0037657 CDS 6419 6484 6419 ID=Msa1456660-mRNA-1:cds;Parent=Msa1456660-mRNA-1
tig0037657 CDS 6126 6194 6126 ID=Msa1456660-mRNA-1:cds;Parent=Msa1456660-mRNA-1
tig0037657 CDS 5961 6032 5961 ID=Msa1456660-mRNA-1:cds;Parent=Msa1456660-mRNA-1
tig0037657 CDS 5457 5585 5457 ID=Msa1456660-mRNA-1:cds;Parent=Msa1456660-mRNA-1
tig0037657 three_prime_UTR 5290 5456 5290 ID=Msa1456660-mRNA-1:three_prime_utr;Parent=Msa1456660-mRNA-1
tig0037657 three_prime_UTR 5073 5269 5073 ID=Msa1456660-mRNA-1:three_prime_utr;Parent=Msa1456660-mRNA-1
Gene Sequence

>Msa1456660

ATGGATCCAGCGTATCATCTCCCGGAGATCTGGCAGTCCAGAAGACCGCAGTTTAGCCCCAGCTTCGGAGATTTTCCAATGGAAGATCTCAGTGCTGAACAAAAACCACTGAACCATGGCCGGAAAAGTAGAATAGAGACGAAGGAAGATTCAGTTAAATGCGTTTCTACCGGCAATGGTGGGGGCCGTGGCAATTCCAACAACGTGAATGATGATGGTGGTAGTGGTGATGGAAAAAGGAGTAAGACATCAGGGAACAGTAAAGGAGAAGAAAACAGTTCAGGAAAGCATGCGGAAGAAACTAATGTTGAGCCACATCCTAAGAAAGACTACATCCATGTTCGAGCCAGAAGGGGTCAAGCTACTGATAGCCATAGTCTTGCAGAAAGAGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAACTCTTCAGGATTTAGTCCCTGGTTGCAACAAGGTTATTGGAAAAGCATTGGTCCTTGATGAGATAATCAATTATATCCAATCACTTCACCACCAAGTAGAGTTTTTGTCGATGAAGCTTGAAGCAGTAAATTCAAGACCAACACCTGGTATTGAGGCGTTTCCTCCTAAAACTTTTGATCAGCAAACATATGATACAACCACCATACCATTCGCTTCACAAGCTACAAGAGAGTACAGCCGAGGTGCATCACCAGAGTGGTTACATATGCAGCTGGGAGGTGGTTTTGAAAGAACAGCATAA
Protein sequence

>Msa1456660

MDPAYHLPEIWQSRRPQFSPSFGDFPMEDLSAEQKPLNHGRKSRIETKEDSVKCVSTGNGGGRGNSNNVNDDGGSGDGKRSKTSGNSKGEENSSGKHAEETNVEPHPKKDYIHVRARRGQATDSHSLAERARREKISERMKTLQDLVPGCNKVIGKALVLDEIINYIQSLHHQVEFLSMKLEAVNSRPTPGIEAFPPKTFDQQTYDTTTIPFASQATREYSRGASPEWLHMQLGGGFERTA*