Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460570 | RHN56939.1 | 86.667 | 150 | 11 | 3 | 1 | 149 | 5 | 146 | 6.98e-87 | 260 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460570 | sp|Q9FNV9|MY113_ARATH | 56.944 | 72 | 28 | 2 | 1 | 69 | 77 | 148 | 2.72e-15 | 73.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460570 | A0A396HUE6 | 86.667 | 150 | 11 | 3 | 1 | 149 | 5 | 146 | 3.34e-87 | 260 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1460570 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460570 | MtrunA17_Chr5g0434891 | 86.667 | 150 | 11 | 3 | 1 | 149 | 5 | 146 | 6.42e-91 | 260 |
| Msa1460570 | MtrunA17_Chr7g0229131 | 64.000 | 150 | 35 | 4 | 1 | 149 | 41 | 172 | 1.68e-56 | 174 |
| Msa1460570 | MtrunA17_Chr7g0220341 | 53.049 | 164 | 55 | 3 | 1 | 146 | 96 | 255 | 5.20e-50 | 161 |
| Msa1460570 | MtrunA17_Chr7g0228991 | 49.375 | 160 | 64 | 3 | 1 | 146 | 98 | 254 | 6.96e-39 | 132 |
| Msa1460570 | MtrunA17_Chr8g0360111 | 49.693 | 163 | 48 | 5 | 1 | 146 | 94 | 239 | 1.94e-35 | 123 |
| Msa1460570 | MtrunA17_Chr7g0229101 | 56.618 | 136 | 42 | 3 | 1 | 136 | 41 | 159 | 5.07e-35 | 120 |
| Msa1460570 | MtrunA17_Chr5g0434931 | 51.034 | 145 | 66 | 3 | 1 | 144 | 104 | 244 | 1.45e-34 | 121 |
| Msa1460570 | MtrunA17_Chr5g0434911 | 58.140 | 129 | 47 | 4 | 1 | 128 | 90 | 212 | 1.35e-33 | 119 |
| Msa1460570 | MtrunA17_Chr5g0434841 | 44.231 | 156 | 56 | 4 | 1 | 126 | 89 | 243 | 4.91e-30 | 110 |
| Msa1460570 | MtrunA17_Chr5g0434831 | 54.206 | 107 | 44 | 2 | 1 | 103 | 76 | 181 | 7.42e-30 | 107 |
| Msa1460570 | MtrunA17_Chr5g0434901 | 68.354 | 79 | 22 | 1 | 1 | 79 | 42 | 117 | 2.51e-23 | 88.2 |
| Msa1460570 | MtrunA17_Chr5g0434871 | 75.385 | 65 | 14 | 1 | 1 | 65 | 9 | 71 | 2.29e-18 | 74.3 |
| Msa1460570 | MtrunA17_Chr7g0240821 | 51.786 | 56 | 27 | 0 | 1 | 56 | 70 | 125 | 9.93e-14 | 65.9 |
| Msa1460570 | MtrunA17_Chr4g0070641 | 45.070 | 71 | 37 | 1 | 1 | 71 | 81 | 149 | 1.24e-13 | 64.7 |
| Msa1460570 | MtrunA17_Chr4g0070611 | 45.070 | 71 | 37 | 1 | 1 | 71 | 81 | 149 | 1.69e-13 | 66.2 |
| Msa1460570 | MtrunA17_Chr4g0055981 | 39.583 | 96 | 51 | 3 | 1 | 96 | 124 | 212 | 2.81e-12 | 62.0 |
| Msa1460570 | MtrunA17_Chr1g0187761 | 53.846 | 52 | 24 | 0 | 1 | 52 | 81 | 132 | 3.23e-12 | 62.0 |
| Msa1460570 | MtrunA17_Chr7g0274581 | 70.270 | 37 | 11 | 0 | 1 | 37 | 81 | 117 | 4.27e-12 | 61.2 |
| Msa1460570 | MtrunA17_Chr2g0323351 | 49.153 | 59 | 22 | 1 | 1 | 51 | 81 | 139 | 2.79e-11 | 59.3 |
| Msa1460570 | MtrunA17_Chr3g0131121 | 52.941 | 51 | 22 | 1 | 1 | 49 | 81 | 131 | 2.95e-11 | 59.7 |
| Msa1460570 | MtrunA17_Chr1g0203411 | 70.270 | 37 | 11 | 0 | 1 | 37 | 81 | 117 | 3.07e-11 | 59.7 |
| Msa1460570 | MtrunA17_Chr8g0346561 | 67.568 | 37 | 12 | 0 | 1 | 37 | 81 | 117 | 3.50e-11 | 59.7 |
| Msa1460570 | MtrunA17_Chr1g0167661 | 62.500 | 40 | 15 | 0 | 1 | 40 | 81 | 120 | 3.71e-11 | 59.3 |
| Msa1460570 | MtrunA17_Chr7g0275911 | 56.098 | 41 | 18 | 0 | 1 | 41 | 81 | 121 | 3.84e-11 | 59.3 |
| Msa1460570 | MtrunA17_Chr3g0121241 | 70.588 | 34 | 10 | 0 | 1 | 34 | 111 | 144 | 8.95e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1460570 | AT1G66370.1 | 56.944 | 72 | 28 | 2 | 1 | 69 | 77 | 148 | 2.76e-16 | 73.6 |
| Msa1460570 | AT1G56650.1 | 50.725 | 69 | 32 | 1 | 6 | 72 | 82 | 150 | 8.82e-13 | 63.9 |
| Msa1460570 | AT5G14750.4 | 63.043 | 46 | 16 | 1 | 1 | 46 | 24 | 68 | 3.69e-12 | 60.5 |
| Msa1460570 | AT5G14750.2 | 63.043 | 46 | 16 | 1 | 1 | 46 | 24 | 68 | 3.69e-12 | 60.5 |
| Msa1460570 | AT3G23250.2 | 58.824 | 51 | 18 | 1 | 1 | 48 | 24 | 74 | 5.04e-12 | 61.6 |
| Msa1460570 | AT5G14750.3 | 63.043 | 46 | 16 | 1 | 1 | 46 | 51 | 95 | 8.44e-12 | 60.1 |
| Msa1460570 | AT5G52600.1 | 50.877 | 57 | 21 | 1 | 1 | 57 | 81 | 130 | 1.28e-11 | 60.1 |
| Msa1460570 | AT3G23250.1 | 58.824 | 51 | 18 | 1 | 1 | 48 | 81 | 131 | 1.70e-11 | 60.8 |
| Msa1460570 | AT1G66390.1 | 50.000 | 70 | 32 | 1 | 6 | 72 | 82 | 151 | 1.73e-11 | 60.5 |
| Msa1460570 | AT2G16720.1 | 46.970 | 66 | 25 | 1 | 1 | 56 | 81 | 146 | 1.92e-11 | 60.5 |
| Msa1460570 | AT3G13540.1 | 37.383 | 107 | 57 | 4 | 1 | 100 | 92 | 195 | 1.93e-11 | 60.5 |
| Msa1460570 | AT1G06180.1 | 55.319 | 47 | 21 | 0 | 1 | 47 | 81 | 127 | 2.04e-11 | 60.1 |
| Msa1460570 | AT5G14750.1 | 63.043 | 46 | 16 | 1 | 1 | 46 | 85 | 129 | 2.41e-11 | 59.3 |
| Msa1460570 | AT5G35550.2 | 41.558 | 77 | 40 | 1 | 1 | 72 | 86 | 162 | 4.20e-11 | 59.3 |
| Msa1460570 | AT1G22640.3 | 67.647 | 34 | 11 | 0 | 1 | 34 | 24 | 57 | 4.56e-11 | 58.5 |
| Msa1460570 | AT3G27920.1 | 50.000 | 58 | 26 | 1 | 1 | 55 | 83 | 140 | 7.61e-11 | 58.5 |
| Msa1460570 | AT5G35550.1 | 41.558 | 77 | 40 | 1 | 1 | 72 | 83 | 159 | 7.63e-11 | 58.9 |
Find 24 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTTGCTTCAAATAAATT+TGG | 0.216237 | tig0040973:+412 | Msa1460570:CDS |
| CCACATTGCACACATAATAT+TGG | 0.255465 | tig0040973:-535 | Msa1460570:intergenic |
| CACATTGCACACATAATATT+GGG | 0.287887 | tig0040973:-534 | Msa1460570:intergenic |
| GTTGATTGCTGCAAGACTTC+AGG | 0.307631 | tig0040973:+186 | Msa1460570:CDS |
| TGAGCTTTAATAATTTCCTT+AGG | 0.337788 | tig0040973:-307 | Msa1460570:intergenic |
| AGTACAAGAGCAGAACTATA+AGG | 0.340917 | tig0040973:+567 | Msa1460570:CDS |
| CTCGAACATCCTTCTTCAAA+TGG | 0.347213 | tig0040973:-246 | Msa1460570:intergenic |
| ATTGGGGCATTACCTAGATT+TGG | 0.347306 | tig0040973:-517 | Msa1460570:intergenic |
| CTCATTCACCTTGGTTGAAT+GGG | 0.362154 | tig0040973:+365 | Msa1460570:CDS |
| GCTCATTCACCTTGGTTGAA+TGG | 0.375142 | tig0040973:+364 | Msa1460570:CDS |
| TCTAAATCACAACCAAATCT+AGG | 0.397333 | tig0040973:+505 | Msa1460570:CDS |
| GATGAGTCATCATGATATCT+AGG | 0.447163 | tig0040973:-442 | Msa1460570:intergenic |
| ACTATGGTTCCCGATCAAAT+TGG | 0.451524 | tig0040973:+469 | Msa1460570:CDS |
| TGATGACTCATCAGACACTA+TGG | 0.451759 | tig0040973:+453 | Msa1460570:CDS |
| GAGCTTTAATAATTTCCTTA+GGG | 0.457767 | tig0040973:-306 | Msa1460570:intergenic |
| TTGATTGCTGCAAGACTTCA+GGG | 0.467852 | tig0040973:+187 | Msa1460570:CDS |
| TCGAACATCCTTCTTCAAAT+GGG | 0.502520 | tig0040973:-245 | Msa1460570:intergenic |
| ACAATCTCTACCAATTTGAT+CGG | 0.538699 | tig0040973:-479 | Msa1460570:intergenic |
| TTGGCACACCCATTTGAAGA+AGG | 0.541157 | tig0040973:+237 | Msa1460570:CDS |
| CAATCTCTACCAATTTGATC+GGG | 0.564185 | tig0040973:-478 | Msa1460570:intergenic |
| TTATGTTTCCCATTCAACCA+AGG | 0.628157 | tig0040973:-373 | Msa1460570:intergenic |
| CATTTGAAGAAGGATGTTCG+AGG | 0.648298 | tig0040973:+247 | Msa1460570:CDS |
| ACATTGCACACATAATATTG+GGG | 0.657462 | tig0040973:-533 | Msa1460570:intergenic |
| CCAATATTATGTGTGCAATG+TGG | 0.711847 | tig0040973:+535 | Msa1460570:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAATTTCTTTTTTTTC+TGG | + | tig0040973:51-70 | Msa1460570:intron | 10.0% |
| !! | TATGATACATATAATATAGA+TGG | + | tig0040973:161-180 | Msa1460570:intron | 15.0% |
| !! | ATATTTGCTTCAAATAAATT+TGG | + | tig0040973:412-431 | Msa1460570:CDS | 15.0% |
| !!! | AATTTTAATTTGGCTCTATT+TGG | - | tig0040973:36-55 | Msa1460570:intergenic | 20.0% |
| !!! | TTAATTTCTTTTTTTTCTGG+AGG | + | tig0040973:54-73 | Msa1460570:intron | 20.0% |
| !!! | TAATTTCTTTTTTTTCTGGA+GGG | + | tig0040973:55-74 | Msa1460570:intron | 20.0% |
| ! | CTAATGATGTGAAAAACTAT+TGG | + | tig0040973:218-237 | Msa1460570:CDS | 25.0% |
| !! | GAGCTTTAATAATTTCCTTA+GGG | - | tig0040973:309-328 | Msa1460570:intergenic | 25.0% |
| !! | TGAGCTTTAATAATTTCCTT+AGG | - | tig0040973:310-329 | Msa1460570:intergenic | 25.0% |
| !! | AGAACTATAAGGTTTTCAAA+TGG | + | tig0040973:578-597 | Msa1460570:CDS | 25.0% |
| GAAAGAAAAAGATAACCCTA+AGG | + | tig0040973:291-310 | Msa1460570:CDS | 30.0% | |
| ACAATCTCTACCAATTTGAT+CGG | - | tig0040973:482-501 | Msa1460570:intergenic | 30.0% | |
| TCTAAATCACAACCAAATCT+AGG | + | tig0040973:505-524 | Msa1460570:CDS | 30.0% | |
| ACATTGCACACATAATATTG+GGG | - | tig0040973:536-555 | Msa1460570:intergenic | 30.0% | |
| CACATTGCACACATAATATT+GGG | - | tig0040973:537-556 | Msa1460570:intergenic | 30.0% | |
| TCGAACATCCTTCTTCAAAT+GGG | - | tig0040973:248-267 | Msa1460570:intergenic | 35.0% | |
| GAATGAGCTGAAAAAGTTAG+TGG | - | tig0040973:352-371 | Msa1460570:intergenic | 35.0% | |
| TTATGTTTCCCATTCAACCA+AGG | - | tig0040973:376-395 | Msa1460570:intergenic | 35.0% | |
| GATGAGTCATCATGATATCT+AGG | - | tig0040973:445-464 | Msa1460570:intergenic | 35.0% | |
| CAATCTCTACCAATTTGATC+GGG | - | tig0040973:481-500 | Msa1460570:intergenic | 35.0% | |
| CCACATTGCACACATAATAT+TGG | - | tig0040973:538-557 | Msa1460570:intergenic | 35.0% | |
| CCAATATTATGTGTGCAATG+TGG | + | tig0040973:535-554 | Msa1460570:CDS | 35.0% | |
| AGTACAAGAGCAGAACTATA+AGG | + | tig0040973:567-586 | Msa1460570:CDS | 35.0% | |
| TTGATTGCTGCAAGACTTCA+GGG | + | tig0040973:187-206 | Msa1460570:CDS | 40.0% | |
| CTCGAACATCCTTCTTCAAA+TGG | - | tig0040973:249-268 | Msa1460570:intergenic | 40.0% | |
| CATTTGAAGAAGGATGTTCG+AGG | + | tig0040973:247-266 | Msa1460570:CDS | 40.0% | |
| !!! | CTTTTTCAGCTCATTCACCT+TGG | + | tig0040973:356-375 | Msa1460570:CDS | 40.0% |
| CTCATTCACCTTGGTTGAAT+GGG | + | tig0040973:365-384 | Msa1460570:CDS | 40.0% | |
| TGATGACTCATCAGACACTA+TGG | + | tig0040973:453-472 | Msa1460570:CDS | 40.0% | |
| ACTATGGTTCCCGATCAAAT+TGG | + | tig0040973:469-488 | Msa1460570:CDS | 40.0% | |
| ! | ATTGGGGCATTACCTAGATT+TGG | - | tig0040973:520-539 | Msa1460570:intergenic | 40.0% |
| TACACAACCTCCTAGGGAAT+AGG | + | tig0040973:3-22 | Msa1460570:CDS | 45.0% | |
| AAGCTTACCTATTCCCTAGG+AGG | - | tig0040973:13-32 | Msa1460570:intergenic | 45.0% | |
| TGGAAGCTTACCTATTCCCT+AGG | - | tig0040973:16-35 | Msa1460570:intergenic | 45.0% | |
| GTTGATTGCTGCAAGACTTC+AGG | + | tig0040973:186-205 | Msa1460570:CDS | 45.0% | |
| TTGGCACACCCATTTGAAGA+AGG | + | tig0040973:237-256 | Msa1460570:CDS | 45.0% | |
| ! | GCTGAAAAAGTTAGTGGACG+AGG | - | tig0040973:346-365 | Msa1460570:intergenic | 45.0% |
| GCTCATTCACCTTGGTTGAA+TGG | + | tig0040973:364-383 | Msa1460570:CDS | 45.0% | |
| !!! | AAAAGAAATTAATTTTAATT+TGG | - | tig0040973:46-65 | Msa1460570:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0040973 | gene | 2 | 606 | 2 | ID=Msa1460570;Name=Msa1460570 |
| tig0040973 | mRNA | 2 | 606 | 2 | ID=Msa1460570-mRNA-1;Parent=Msa1460570;Name=Msa1460570-mRNA-1;_AED=0.02;_eAED=0.03;_QI=0|0|0|1|0|0.5|2|0|149 |
| tig0040973 | exon | 2 | 24 | 2 | ID=Msa1460570-mRNA-1:exon:22800;Parent=Msa1460570-mRNA-1 |
| tig0040973 | exon | 180 | 606 | 180 | ID=Msa1460570-mRNA-1:exon:22801;Parent=Msa1460570-mRNA-1 |
| tig0040973 | CDS | 2 | 24 | 2 | ID=Msa1460570-mRNA-1:cds;Parent=Msa1460570-mRNA-1 |
| tig0040973 | CDS | 180 | 606 | 180 | ID=Msa1460570-mRNA-1:cds;Parent=Msa1460570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
