AlfalfaGEDB | Msa1463360

M. sativa cultivar zhongmu-4 / Msa1463360

KEGG: K02208
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016592 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0097472 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1463360	PNX57153.1	96.330	109	2	1	1	109	34	140	6.72e-70	215

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1463360	sp\|Q336M2\|CDKE1_ORYSJ	75.000	116	20	2	1	107	1	116	1.65e-54	179

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1463360	A0A2K3JSZ0	96.330	109	2	1	1	109	34	140	3.21e-70	215

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1463360	MtrunA17_Chr3g0130781	99.065	107	1	0	1	107	1	107	9.21e-73	223
Msa1463360	MtrunA17_Chr4g0051261	47.826	92	43	3	15	106	1	87	5.27e-20	82.0
Msa1463360	MtrunA17_Chr2g0293491	47.826	92	43	3	15	106	1	87	5.32e-20	82.0
Msa1463360	MtrunA17_Chr3g0093571	33.981	103	51	2	18	106	25	124	3.08e-14	67.0
Msa1463360	MtrunA17_Chr7g0273601	42.424	99	46	4	12	106	101	192	5.43e-14	66.2
Msa1463360	MtrunA17_Chr3g0093391	33.981	103	51	2	18	106	23	122	1.36e-13	65.1
Msa1463360	MtrunA17_Chr3g0099951	43.820	89	43	3	24	110	20	103	1.69e-13	65.1
Msa1463360	MtrunA17_Chr1g0187371	37.895	95	52	3	16	106	16	107	4.03e-13	63.5
Msa1463360	MtrunA17_Chr2g0286421	37.383	107	56	3	4	106	88	187	4.32e-13	63.9
Msa1463360	MtrunA17_Chr4g0002001	37.864	103	50	5	15	106	1	100	5.17e-13	63.2
Msa1463360	MtrunA17_Chr3g0131861	37.374	99	51	3	12	106	91	182	9.34e-13	62.8
Msa1463360	MtrunA17_Chr4g0040141	37.374	99	51	4	12	106	123	214	1.38e-12	62.4
Msa1463360	MtrunA17_Chr6g0483151	39.080	87	48	2	24	110	20	101	1.64e-12	62.0
Msa1463360	MtrunA17_Chr8g0385971	38.542	96	54	3	12	106	84	175	2.03e-12	62.0
Msa1463360	MtrunA17_Chr4g0061141	36.559	93	48	3	18	106	105	190	2.57e-12	61.6
Msa1463360	MtrunA17_Chr4g0066691	37.374	99	51	3	12	106	98	189	4.78e-12	60.8
Msa1463360	MtrunA17_Chr2g0317501	36.364	99	52	3	12	106	128	219	1.36e-11	59.7
Msa1463360	MtrunA17_Chr6g0453711	38.889	90	50	3	18	106	110	195	3.61e-11	58.5
Msa1463360	MtrunA17_Chr7g0218071	36.559	93	48	3	18	106	120	205	4.27e-11	58.2
Msa1463360	MtrunA17_Chr5g0396361	37.500	96	55	3	12	106	134	225	5.19e-11	57.8
Msa1463360	MtrunA17_Chr1g0191221	36.364	99	52	3	12	106	116	207	7.60e-11	57.4
Msa1463360	MtrunA17_Chr2g0324461	40.860	93	47	4	15	106	141	226	7.64e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1463360	AT5G63610.1	85.567	97	12	1	11	107	18	112	2.75e-53	173
Msa1463360	AT3G48750.1	48.913	92	42	3	15	106	1	87	2.71e-19	80.5
Msa1463360	AT1G66750.1	37.736	106	59	3	5	110	2	100	2.32e-14	67.8
Msa1463360	AT5G39420.1	38.947	95	47	3	16	106	103	190	1.09e-13	65.9
Msa1463360	AT1G73690.1	39.080	87	48	3	24	110	17	98	1.57e-13	65.5
Msa1463360	AT2G38620.2	35.922	103	52	3	15	106	1	100	6.79e-13	63.5
Msa1463360	AT2G38620.3	35.922	103	52	3	15	106	1	100	9.66e-13	62.4
Msa1463360	AT2G38620.1	35.922	103	52	3	15	106	1	100	9.66e-13	62.4
Msa1463360	AT1G18670.3	36.559	93	48	3	18	106	131	216	9.87e-13	63.2
Msa1463360	AT1G18670.1	36.559	93	48	3	18	106	131	216	1.07e-12	63.2
Msa1463360	AT1G71530.4	39.583	96	53	3	12	106	141	232	1.10e-12	62.8
Msa1463360	AT3G54180.1	39.604	101	49	3	15	106	1	98	1.11e-12	62.8
Msa1463360	AT1G71530.3	39.583	96	53	3	12	106	141	232	1.18e-12	62.8
Msa1463360	AT5G10270.1	33.654	104	50	3	18	106	26	125	1.19e-12	63.2
Msa1463360	AT1G71530.2	39.583	96	53	3	12	106	141	232	1.20e-12	62.8
Msa1463360	AT1G71530.1	39.583	96	53	3	12	106	141	232	1.25e-12	62.8
Msa1463360	AT1G20930.1	38.542	96	52	4	15	106	13	105	1.32e-12	62.4
Msa1463360	AT1G18670.2	36.559	93	48	3	18	106	131	216	1.95e-12	62.4
Msa1463360	AT5G64960.1	33.654	104	50	3	18	106	26	125	2.33e-12	62.0
Msa1463360	AT5G50860.2	38.542	96	54	3	12	106	108	199	4.45e-12	61.2
Msa1463360	AT5G50860.1	38.542	96	54	3	12	106	108	199	4.54e-12	61.2
Msa1463360	AT1G53050.2	35.354	99	53	3	12	106	128	219	5.77e-12	61.2
Msa1463360	AT1G53050.1	35.354	99	53	3	12	106	128	219	5.77e-12	61.2
Msa1463360	AT1G18040.1	37.931	87	49	3	24	110	18	99	6.39e-12	60.8
Msa1463360	AT1G67580.1	33.696	92	58	1	15	106	403	491	7.26e-12	60.8
Msa1463360	AT1G67580.2	33.696	92	58	1	15	106	403	491	7.26e-12	60.8
Msa1463360	AT1G54610.1	36.697	109	58	3	2	106	102	203	9.12e-12	60.5
Msa1463360	AT1G54610.3	36.697	109	58	3	2	106	102	203	9.12e-12	60.5
Msa1463360	AT1G54610.2	36.697	109	58	3	2	106	102	203	9.12e-12	60.5
Msa1463360	AT4G10010.2	39.583	96	53	3	12	106	150	241	9.79e-12	60.5
Msa1463360	AT3G01085.3	40.000	85	46	3	23	106	120	200	3.56e-11	58.9
Msa1463360	AT3G01085.1	40.000	85	46	3	23	106	120	200	3.63e-11	58.9
Msa1463360	AT3G01085.2	40.000	85	46	3	23	106	120	200	3.63e-11	58.9
Msa1463360	AT1G33770.1	38.542	96	54	3	12	106	135	226	5.55e-11	58.2

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGACACCATCGCCGTCTT+TGG	0.305118	tig0043161:-10383	None:intergenic
CCACTCTGGTTTGTTGCTGT+TGG	0.338552	tig0043161:-10239	None:intergenic
TACAATCACCAAGCGGAAAC+TGG	0.389878	tig0043161:-10186	None:intergenic
GGATTGTTTGAATTTCTTGA+TGG	0.419484	tig0043161:-10362	None:intergenic
CTTCAACAGTACGATCTGAT+TGG	0.436864	tig0043161:+10262	Msa1463360:CDS
CACCAAGCGGAAACTGGTTA+AGG	0.456837	tig0043161:-10180	None:intergenic
AATATAGTACCATGATTTCA+CGG	0.482098	tig0043161:-10417	None:intergenic
TTCAACAGTACGATCTGATT+GGG	0.482436	tig0043161:+10263	Msa1463360:CDS
GTATCGGTATACTCACATAA+AGG	0.485984	tig0043161:-10695	None:intergenic
TACCGCCATCCGTGAAATCA+TGG	0.489893	tig0043161:+10408	Msa1463360:CDS
ACGGTCTCGTCTTCCTCGCT+CGG	0.502253	tig0043161:+10305	Msa1463360:CDS
TACGATCTGATTGGGAAGAT+CGG	0.526902	tig0043161:+10271	Msa1463360:CDS
ATCGAGTTCGCGATGGCAGA+CGG	0.532852	tig0043161:+10208	Msa1463360:exon
AACCTTAACCAGTTTCCGCT+TGG	0.541480	tig0043161:+10178	Msa1463360:five_prime_UTR
TTTGAATTTCTTGATGGCGA+TGG	0.548927	tig0043161:-10356	None:intergenic
TCGTACTGTTGAAGCCACTC+TGG	0.554430	tig0043161:-10253	None:intergenic
TTTGATAATGCAGTTGCTGA+GGG	0.557254	tig0043161:+10578	Msa1463360:intron
ATTTGATAATGCAGTTGCTG+AGG	0.560005	tig0043161:+10577	Msa1463360:intron
ATCAAATCCGCCACAAACCG+CGG	0.561014	tig0043161:+10328	Msa1463360:CDS
CTGATTGGGAAGATCGGAGA+AGG	0.584342	tig0043161:+10277	Msa1463360:CDS
CTGGTTTGTTGCTGTTGGAG+CGG	0.588436	tig0043161:-10234	None:intergenic
TAGTACCATGATTTCACGGA+TGG	0.591974	tig0043161:-10413	None:intergenic
AAACAATCCAAAGACGGCGA+TGG	0.604550	tig0043161:+10376	Msa1463360:CDS
GTCATGTTCAGCATAATCAA+AGG	0.611548	tig0043161:-10673	None:intergenic
TACCATGATTTCACGGATGG+CGG	0.618669	tig0043161:-10410	None:intergenic
ATGATTTCACGGATGGCGGT+AGG	0.629806	tig0043161:-10406	None:intergenic
AAGATCGGAGAAGGAACCTA+CGG	0.635512	tig0043161:+10286	Msa1463360:CDS
GGCGAGATAGAGAGACATGT+CGG	0.639777	tig0043161:-10652	None:intergenic
AAATTCAAACAATCCAAAGA+CGG	0.640296	tig0043161:+10370	Msa1463360:CDS
TGATTGTATCGAGTTCGCGA+TGG	0.641694	tig0043161:+10201	Msa1463360:exon
TGGCGGATTTGATCCGAGCG+AGG	0.654084	tig0043161:-10318	None:intergenic
AGCGAGGAAGACGAGACCGT+AGG	0.699028	tig0043161:-10302	None:intergenic
AACTCGATACAATCACCAAG+CGG	0.705373	tig0043161:-10193	None:intergenic
CCAACAGCAACAAACCAGAG+TGG	0.762734	tig0043161:+10239	Msa1463360:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTACAATTTAAAAATGAGA+GGG	-	tig0043161:10450-10469	None:intergenic	20.0%
!!	TTCTACAATTTAAAAATGAG+AGG	-	tig0043161:10451-10470	None:intergenic	20.0%
!	AAATTCAAACAATCCAAAGA+CGG	+	tig0043161:10370-10389	Msa1463360:CDS	25.0%
!	AATATAGTACCATGATTTCA+CGG	-	tig0043161:10420-10439	None:intergenic	25.0%
!	GTAGAATTCACTCAAATTAT+TGG	+	tig0043161:10465-10484	Msa1463360:intron	25.0%
	GGATTGTTTGAATTTCTTGA+TGG	-	tig0043161:10365-10384	None:intergenic	30.0%
	TTCAACAGTACGATCTGATT+GGG	+	tig0043161:10263-10282	Msa1463360:CDS	35.0%
!!	TTTGAATTTCTTGATGGCGA+TGG	-	tig0043161:10359-10378	None:intergenic	35.0%
!!	ATTTGATAATGCAGTTGCTG+AGG	+	tig0043161:10577-10596	Msa1463360:intron	35.0%
!!	TTTGATAATGCAGTTGCTGA+GGG	+	tig0043161:10578-10597	Msa1463360:intron	35.0%
	GTCATGTTCAGCATAATCAA+AGG	-	tig0043161:10676-10695	None:intergenic	35.0%
	AACTCGATACAATCACCAAG+CGG	-	tig0043161:10196-10215	None:intergenic	40.0%
	CTTCAACAGTACGATCTGAT+TGG	+	tig0043161:10262-10281	Msa1463360:CDS	40.0%
	TACGATCTGATTGGGAAGAT+CGG	+	tig0043161:10271-10290	Msa1463360:CDS	40.0%
	TAGTACCATGATTTCACGGA+TGG	-	tig0043161:10416-10435	None:intergenic	40.0%
	AACCTTAACCAGTTTCCGCT+TGG	+	tig0043161:10178-10197	Msa1463360:five_prime_UTR	45.0%
	TACAATCACCAAGCGGAAAC+TGG	-	tig0043161:10189-10208	None:intergenic	45.0%
	TGATTGTATCGAGTTCGCGA+TGG	+	tig0043161:10201-10220	Msa1463360:exon	45.0%
	AAGATCGGAGAAGGAACCTA+CGG	+	tig0043161:10286-10305	Msa1463360:CDS	45.0%
	AAACAATCCAAAGACGGCGA+TGG	+	tig0043161:10376-10395	Msa1463360:CDS	45.0%
	TACCATGATTTCACGGATGG+CGG	-	tig0043161:10413-10432	None:intergenic	45.0%
	CACCAAGCGGAAACTGGTTA+AGG	-	tig0043161:10183-10202	None:intergenic	50.0%
!!	CTGGTTTGTTGCTGTTGGAG+CGG	-	tig0043161:10237-10256	None:intergenic	50.0%
!!	CCACTCTGGTTTGTTGCTGT+TGG	-	tig0043161:10242-10261	None:intergenic	50.0%
	CCAACAGCAACAAACCAGAG+TGG	+	tig0043161:10239-10258	Msa1463360:CDS	50.0%
	TCGTACTGTTGAAGCCACTC+TGG	-	tig0043161:10256-10275	None:intergenic	50.0%
	CTGATTGGGAAGATCGGAGA+AGG	+	tig0043161:10277-10296	Msa1463360:CDS	50.0%
	ATCAAATCCGCCACAAACCG+CGG	+	tig0043161:10328-10347	Msa1463360:CDS	50.0%
!!	GATGGATTTTCCGCGGTTTG+TGG	-	tig0043161:10341-10360	None:intergenic	50.0%
!!	TGATGGCGATGGATTTTCCG+CGG	-	tig0043161:10348-10367	None:intergenic	50.0%
!	ATGATTTCACGGATGGCGGT+AGG	-	tig0043161:10409-10428	None:intergenic	50.0%
	TACCGCCATCCGTGAAATCA+TGG	+	tig0043161:10408-10427	Msa1463360:CDS	50.0%
	GGCGAGATAGAGAGACATGT+CGG	-	tig0043161:10655-10674	None:intergenic	50.0%
	ATCGAGTTCGCGATGGCAGA+CGG	+	tig0043161:10208-10227	Msa1463360:exon	55.0%
!	GGATTTTCCGCGGTTTGTGG+CGG	-	tig0043161:10338-10357	None:intergenic	55.0%
	AGAGACACCATCGCCGTCTT+TGG	-	tig0043161:10386-10405	None:intergenic	55.0%
	AGCGAGGAAGACGAGACCGT+AGG	-	tig0043161:10305-10324	None:intergenic	60.0%
	ACGGTCTCGTCTTCCTCGCT+CGG	+	tig0043161:10305-10324	Msa1463360:CDS	60.0%
!!	TGGCGGATTTGATCCGAGCG+AGG	-	tig0043161:10321-10340	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0043161	gene	10169	10710	10169	ID=Msa1463360;Name=Msa1463360
tig0043161	mRNA	10169	10710	10169	ID=Msa1463360-mRNA-1;Parent=Msa1463360;Name=Msa1463360-mRNA-1;_AED=0.00;_eAED=0.00;_QI=51\|1\|1\|1\|1\|1\|2\|0\|110
tig0043161	exon	10169	10429	10169	ID=Msa1463360-mRNA-1:exon:24000;Parent=Msa1463360-mRNA-1
tig0043161	exon	10591	10710	10591	ID=Msa1463360-mRNA-1:exon:24001;Parent=Msa1463360-mRNA-1
tig0043161	five_prime_UTR	10169	10219	10169	ID=Msa1463360-mRNA-1:five_prime_utr;Parent=Msa1463360-mRNA-1
tig0043161	CDS	10220	10429	10220	ID=Msa1463360-mRNA-1:cds;Parent=Msa1463360-mRNA-1
tig0043161	CDS	10591	10710	10591	ID=Msa1463360-mRNA-1:cds;Parent=Msa1463360-mRNA-1

Gene Sequence	>Msa1463360 ATGGCAGACGGTAACCGCTCCAACAGCAACAAACCAGAGTGGCTTCAACAGTACGATCTGATTGGGAAGATCGGAGAAGGAACCTACGGTCTCGTCTTCCTCGCTCGGATCAAATCCGCCACAAACCGCGGAAAATCCATCGCCATCAAGAAATTCAAACAATCCAAAGACGGCGATGGTGTCTCTCCTACCGCCATCCGTGAAATCATGTTGCTGAGGGAGATAACACATGAAAATGTTGTCAAGCTTGTGAATGTTCACATCAATCATACCGACATGTCTCTCTATCTCGCCTTTGATTATGCTGAACATGACCTTTATGTGAGTATA
Protein sequence	>Msa1463360 MADGNRSNSNKPEWLQQYDLIGKIGEGTYGLVFLARIKSATNRGKSIAIKKFKQSKDGDGVSPTAIREIMLLREITHENVVKLVNVHINHTDMSLYLAFDYAEHDLYVSI