AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1465560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465560 MtrunA17_Chr5g0394631 85.204 196 24 3 1 193 208 401 1.77e-114 332
Msa1465560 MtrunA17_Chr5g0394631 66.484 182 54 3 9 184 1 181 3.31e-75 232
Msa1465560 MtrunA17_Chr5g0394621 70.681 191 49 3 9 193 1 190 8.33e-89 259
Msa1465560 MtrunA17_Chr1g0204951 61.538 182 63 4 16 193 12 190 4.79e-74 221
Msa1465560 MtrunA17_Chr1g0204971 59.659 176 66 3 20 193 18 190 2.83e-71 214
Msa1465560 MtrunA17_Chr7g0223361 45.349 172 89 3 20 190 3 170 2.54e-42 140
Msa1465560 MtrunA17_Chr7g0223351 44.379 169 86 4 24 190 7 169 1.97e-39 133
Msa1465560 MtrunA17_Chr7g0273661 43.312 157 83 3 21 175 4 156 2.96e-37 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465560 AT2G32030.1 48.705 193 86 6 1 190 3 185 8.31e-54 171
Msa1465560 AT2G32020.1 51.765 170 72 5 24 190 18 180 7.51e-52 165
Msa1465560 AT3G22560.1 38.857 175 99 4 20 190 2 172 8.90e-34 119

Find 38 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTGATGTGGATAATTTA+AGG 0.203228 tig0045301:-905 Msa1465560:CDS
CAAATCAATGATCTCTTCTT+TGG 0.204931 tig0045301:+1292 Msa1465560:intergenic
GGACTTTGTTCCTTTGGTTT+TGG 0.218717 tig0045301:-505 Msa1465560:CDS
GTTGATGCCTTGGTCTTTGC+TGG 0.262298 tig0045301:+1169 Msa1465560:intergenic
GCTTGAAGCCTTTCCAAGTA+TGG 0.276638 tig0045301:+930 Msa1465560:intergenic
TGCCATAACGATCATGCTAT+TGG 0.288303 tig0045301:-1101 Msa1465560:CDS
ATTGCAGGACTTTGTTCCTT+TGG 0.289499 tig0045301:-511 Msa1465560:intron
GCAGAACTTGGGTATGTTCT+AGG 0.307665 tig0045301:-1026 Msa1465560:CDS
ATGTTCTAGGCTCTAAATAC+TGG 0.313478 tig0045301:-1013 Msa1465560:CDS
CCTTGGTCTTTGCTGGTGTA+AGG 0.327596 tig0045301:+1176 Msa1465560:intergenic
GAAAAGATCGGAGTGTGATT+TGG 0.386444 tig0045301:+1267 Msa1465560:intergenic
ACCTCGATGATCTCATGGTT+TGG 0.401354 tig0045301:-1235 Msa1465560:CDS
GGCTCTAAATACTGGGGTAA+AGG 0.409375 tig0045301:-1005 Msa1465560:CDS
ATTCAGTGAATTTCCATACT+TGG 0.413337 tig0045301:-943 Msa1465560:CDS
GCCATAACGATCATGCTATT+GGG 0.423146 tig0045301:-1100 Msa1465560:CDS
TTCTGACCTCGATGATCTCA+TGG 0.433346 tig0045301:-1240 Msa1465560:CDS
GCTCTAAATACTGGGGTAAA+GGG 0.442106 tig0045301:-1004 Msa1465560:CDS
TGAGTTCACTTGATGATAAG+AGG 0.442621 tig0045301:-1061 Msa1465560:CDS
CAAAGAGTAGTTGAGAAAGT+TGG 0.450786 tig0045301:-879 Msa1465560:CDS
AGGATCGCATGTGCAGAACT+TGG 0.454084 tig0045301:-1038 Msa1465560:CDS
ACCCAATAGCATGATCGTTA+TGG 0.457877 tig0045301:+1099 Msa1465560:intergenic
CTACTGATCTTAAACTAATG+AGG 0.474389 tig0045301:-776 Msa1465560:CDS
GTAAGGTTCACCCCAGAACT+TGG 0.480302 tig0045301:+1193 Msa1465560:intergenic
TAAACTAATGAGGATGTTCA+AGG 0.483858 tig0045301:-766 Msa1465560:intron
GTGAATTTCCATACTTGGAA+AGG 0.490341 tig0045301:-938 Msa1465560:CDS
TGCTGTGAAACAAATGGTTA+AGG 0.500098 tig0045301:-970 Msa1465560:CDS
TGTTCTAGGCTCTAAATACT+GGG 0.526941 tig0045301:-1012 Msa1465560:CDS
CTTATCATCAAGTGAACTCA+AGG 0.556721 tig0045301:+1064 Msa1465560:intergenic
GCTTCAAGCTCTTGTTGATG+TGG 0.560091 tig0045301:-916 Msa1465560:CDS
GGATCGCATGTGCAGAACTT+GGG 0.585194 tig0045301:-1037 Msa1465560:CDS
GGTCAGAAAGATGAAAAGAT+CGG 0.592412 tig0045301:+1255 Msa1465560:intergenic
TTTCATGGATCCAAAACCAA+AGG 0.622728 tig0045301:+495 Msa1465560:intergenic
CATTTGTTTCACAGCACATG+TGG 0.625218 tig0045301:+977 Msa1465560:intergenic
CACATGTGCTGTGAAACAAA+TGG 0.647600 tig0045301:-976 Msa1465560:CDS
GTTCACTTGATGATAAGAGG+AGG 0.664638 tig0045301:-1058 Msa1465560:CDS
CCTTACACCAGCAAAGACCA+AGG 0.673207 tig0045301:-1176 Msa1465560:CDS
GTTCTAGGCTCTAAATACTG+GGG 0.712321 tig0045301:-1011 Msa1465560:CDS
GCCAAACCATGAGATCATCG+AGG 0.781144 tig0045301:+1234 Msa1465550:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATCAACTTCATTAAAAATA+TGG - tig0045301:682-701 Msa1465560:intron 20.0%
!!! AGGAAGTATGTATTTTTTAA+AGG - tig0045301:1001-1020 Msa1465560:CDS 20.0%
!! CATCAACTTCATTAAAAATA+TGG - tig0045301:682-701 Msa1465560:intron 20.0%
!!! AGGAAGTATGTATTTTTTAA+AGG - tig0045301:1001-1020 Msa1465560:CDS 20.0%
! TTGTTGATGTGGATAATTTA+AGG - tig0045301:930-949 Msa1465560:CDS 25.0%
!!! AAAGTTGGTTTTCAAAAAGA+GGG - tig0045301:971-990 Msa1465560:CDS 25.0%
! AAACAACTCTAATTAAGAGA+CGG + tig0045301:1193-1212 Msa1465560:intergenic 25.0%
! TTGTTGATGTGGATAATTTA+AGG - tig0045301:930-949 Msa1465560:CDS 25.0%
!!! AAAGTTGGTTTTCAAAAAGA+GGG - tig0045301:971-990 Msa1465560:CDS 25.0%
! AAACAACTCTAATTAAGAGA+CGG + tig0045301:1193-1212 Msa1465560:intergenic 25.0%
CAAATCAATGATCTCTTCTT+TGG + tig0045301:546-565 None:intergenic 30.0%
!!! TTTTAATGAAGTTGATGCCT+TGG + tig0045301:679-698 Msa1465560:intergenic 30.0%
TCATTAAAAATATGGCAAGC+AGG - tig0045301:690-709 Msa1465560:intron 30.0%
ATTCAGTGAATTTCCATACT+TGG - tig0045301:892-911 Msa1465560:CDS 30.0%
!!! GAAAGTTGGTTTTCAAAAAG+AGG - tig0045301:970-989 Msa1465560:CDS 30.0%
CTACTGATCTTAAACTAATG+AGG - tig0045301:1059-1078 Msa1465560:CDS 30.0%
TAAACTAATGAGGATGTTCA+AGG - tig0045301:1069-1088 Msa1465560:CDS 30.0%
ATTGCTGAAATGTAAGTTTG+TGG - tig0045301:1106-1125 Msa1465560:CDS 30.0%
! ATCGTGTTATTCATAGATGA+TGG - tig0045301:1234-1253 Msa1465560:CDS 30.0%
! TCGTGTTATTCATAGATGAT+GGG - tig0045301:1235-1254 Msa1465560:CDS 30.0%
CAAATCAATGATCTCTTCTT+TGG + tig0045301:546-565 Msa1465560:intergenic 30.0%
!!! TTTTAATGAAGTTGATGCCT+TGG + tig0045301:679-698 Msa1465560:intergenic 30.0%
TCATTAAAAATATGGCAAGC+AGG - tig0045301:690-709 Msa1465560:intron 30.0%
ATTCAGTGAATTTCCATACT+TGG - tig0045301:892-911 Msa1465560:CDS 30.0%
!!! GAAAGTTGGTTTTCAAAAAG+AGG - tig0045301:970-989 Msa1465560:CDS 30.0%
CTACTGATCTTAAACTAATG+AGG - tig0045301:1059-1078 Msa1465560:CDS 30.0%
TAAACTAATGAGGATGTTCA+AGG - tig0045301:1069-1088 Msa1465560:CDS 30.0%
ATTGCTGAAATGTAAGTTTG+TGG - tig0045301:1106-1125 Msa1465560:CDS 30.0%
! ATCGTGTTATTCATAGATGA+TGG - tig0045301:1234-1253 Msa1465560:CDS 30.0%
! TCGTGTTATTCATAGATGAT+GGG - tig0045301:1235-1254 Msa1465560:CDS 30.0%
GGTCAGAAAGATGAAAAGAT+CGG + tig0045301:583-602 None:intergenic 35.0%
TTGGCATACTGATGAAAAAG+TGG - tig0045301:619-638 Msa1465560:intron 35.0%
CTTATCATCAAGTGAACTCA+AGG + tig0045301:774-793 Msa1465560:intergenic 35.0%
! TGAGTTCACTTGATGATAAG+AGG - tig0045301:774-793 Msa1465560:CDS 35.0%
!! ATGTTCTAGGCTCTAAATAC+TGG - tig0045301:822-841 Msa1465560:CDS 35.0%
!! TGTTCTAGGCTCTAAATACT+GGG - tig0045301:823-842 Msa1465560:CDS 35.0%
TGCTGTGAAACAAATGGTTA+AGG - tig0045301:865-884 Msa1465560:CDS 35.0%
GTGAATTTCCATACTTGGAA+AGG - tig0045301:897-916 Msa1465560:CDS 35.0%
CAAAGAGTAGTTGAGAAAGT+TGG - tig0045301:956-975 Msa1465560:CDS 35.0%
TTCAAAAAGAGGGAGTTCTT+AGG - tig0045301:981-1000 Msa1465560:CDS 35.0%
TGTAAGTTTGTGGATGTTCA+AGG - tig0045301:1116-1135 Msa1465560:CDS 35.0%
TTCAAGGACATCAACTAATG+AGG - tig0045301:1132-1151 Msa1465560:CDS 35.0%
TCAAGGACATCAACTAATGA+GGG - tig0045301:1133-1152 Msa1465560:CDS 35.0%
ACATCAACTAATGAGGGTTT+GGG - tig0045301:1139-1158 Msa1465560:CDS 35.0%
GGTCAGAAAGATGAAAAGAT+CGG + tig0045301:583-602 Msa1465560:intergenic 35.0%
TTGGCATACTGATGAAAAAG+TGG - tig0045301:619-638 Msa1465560:intron 35.0%
CTTATCATCAAGTGAACTCA+AGG + tig0045301:774-793 Msa1465560:intergenic 35.0%
! TGAGTTCACTTGATGATAAG+AGG - tig0045301:774-793 Msa1465560:CDS 35.0%
!! ATGTTCTAGGCTCTAAATAC+TGG - tig0045301:822-841 Msa1465560:CDS 35.0%
!! TGTTCTAGGCTCTAAATACT+GGG - tig0045301:823-842 Msa1465560:CDS 35.0%
TGCTGTGAAACAAATGGTTA+AGG - tig0045301:865-884 Msa1465560:CDS 35.0%
GTGAATTTCCATACTTGGAA+AGG - tig0045301:897-916 Msa1465560:CDS 35.0%
CAAAGAGTAGTTGAGAAAGT+TGG - tig0045301:956-975 Msa1465560:CDS 35.0%
TTCAAAAAGAGGGAGTTCTT+AGG - tig0045301:981-1000 Msa1465560:CDS 35.0%
TGTAAGTTTGTGGATGTTCA+AGG - tig0045301:1116-1135 Msa1465560:CDS 35.0%
TTCAAGGACATCAACTAATG+AGG - tig0045301:1132-1151 Msa1465560:CDS 35.0%
TCAAGGACATCAACTAATGA+GGG - tig0045301:1133-1152 Msa1465560:CDS 35.0%
ACATCAACTAATGAGGGTTT+GGG - tig0045301:1139-1158 Msa1465560:CDS 35.0%
GAAAAGATCGGAGTGTGATT+TGG + tig0045301:571-590 None:intergenic 40.0%
ATGAAAAAGTGGCCAAGTTC+TGG - tig0045301:630-649 Msa1465560:intron 40.0%
TGAAAAAGTGGCCAAGTTCT+GGG - tig0045301:631-650 Msa1465560:intron 40.0%
TGCCATAACGATCATGCTAT+TGG - tig0045301:734-753 Msa1465560:intron 40.0%
GCCATAACGATCATGCTATT+GGG - tig0045301:735-754 Msa1465560:intron 40.0%
ACCCAATAGCATGATCGTTA+TGG + tig0045301:739-758 Msa1465560:intergenic 40.0%
! GTTCACTTGATGATAAGAGG+AGG - tig0045301:777-796 Msa1465560:CDS 40.0%
!! GTTCTAGGCTCTAAATACTG+GGG - tig0045301:824-843 Msa1465560:CDS 40.0%
! GCTCTAAATACTGGGGTAAA+GGG - tig0045301:831-850 Msa1465560:CDS 40.0%
CATTTGTTTCACAGCACATG+TGG + tig0045301:861-880 Msa1465560:intergenic 40.0%
CACATGTGCTGTGAAACAAA+TGG - tig0045301:859-878 Msa1465560:CDS 40.0%
TAATGAGGATGTTCAAGGTG+AGG - tig0045301:1074-1093 Msa1465560:CDS 40.0%
GACATCAACTAATGAGGGTT+TGG - tig0045301:1138-1157 Msa1465560:CDS 40.0%
GGAGTTTCTCATAAACAGCA+TGG + tig0045301:1172-1191 Msa1465560:intergenic 40.0%
AAAGGAACAAAGTCCTGCAA+TGG + tig0045301:1325-1344 Msa1465560:intergenic 40.0%
ATTGCAGGACTTTGTTCCTT+TGG - tig0045301:1324-1343 Msa1465560:CDS 40.0%
!! GGACTTTGTTCCTTTGGTTT+TGG - tig0045301:1330-1349 Msa1465560:CDS 40.0%
GAAAAGATCGGAGTGTGATT+TGG + tig0045301:571-590 Msa1465560:intergenic 40.0%
ATGAAAAAGTGGCCAAGTTC+TGG - tig0045301:630-649 Msa1465560:intron 40.0%
TGAAAAAGTGGCCAAGTTCT+GGG - tig0045301:631-650 Msa1465560:intron 40.0%
TGCCATAACGATCATGCTAT+TGG - tig0045301:734-753 Msa1465560:intron 40.0%
GCCATAACGATCATGCTATT+GGG - tig0045301:735-754 Msa1465560:intron 40.0%
ACCCAATAGCATGATCGTTA+TGG + tig0045301:739-758 Msa1465560:intergenic 40.0%
! GTTCACTTGATGATAAGAGG+AGG - tig0045301:777-796 Msa1465560:CDS 40.0%
!! GTTCTAGGCTCTAAATACTG+GGG - tig0045301:824-843 Msa1465560:CDS 40.0%
! GCTCTAAATACTGGGGTAAA+GGG - tig0045301:831-850 Msa1465560:CDS 40.0%
CATTTGTTTCACAGCACATG+TGG + tig0045301:861-880 Msa1465560:intergenic 40.0%
CACATGTGCTGTGAAACAAA+TGG - tig0045301:859-878 Msa1465560:CDS 40.0%
TAATGAGGATGTTCAAGGTG+AGG - tig0045301:1074-1093 Msa1465560:CDS 40.0%
GACATCAACTAATGAGGGTT+TGG - tig0045301:1138-1157 Msa1465560:CDS 40.0%
GGAGTTTCTCATAAACAGCA+TGG + tig0045301:1172-1191 Msa1465560:intergenic 40.0%
AAAGGAACAAAGTCCTGCAA+TGG + tig0045301:1325-1344 Msa1465560:intergenic 40.0%
ATTGCAGGACTTTGTTCCTT+TGG - tig0045301:1324-1343 Msa1465560:CDS 40.0%
!! GGACTTTGTTCCTTTGGTTT+TGG - tig0045301:1330-1349 Msa1465560:CDS 40.0%
TTCTGACCTCGATGATCTCA+TGG - tig0045301:595-614 Msa1465560:intron 45.0%
ACCTCGATGATCTCATGGTT+TGG - tig0045301:600-619 Msa1465560:intron 45.0%
GAAAAAGTGGCCAAGTTCTG+GGG - tig0045301:632-651 Msa1465560:intron 45.0%
GCAGAACTTGGGTATGTTCT+AGG - tig0045301:809-828 Msa1465560:CDS 45.0%
!! GGCTCTAAATACTGGGGTAA+AGG - tig0045301:830-849 Msa1465560:CDS 45.0%
GCTTGAAGCCTTTCCAAGTA+TGG + tig0045301:908-927 Msa1465560:intergenic 45.0%
GCTTCAAGCTCTTGTTGATG+TGG - tig0045301:919-938 Msa1465560:CDS 45.0%
! GTTGTGTTTTGCTCCATTGC+AGG - tig0045301:1309-1328 Msa1465560:CDS 45.0%
TTCTGACCTCGATGATCTCA+TGG - tig0045301:595-614 Msa1465560:intron 45.0%
ACCTCGATGATCTCATGGTT+TGG - tig0045301:600-619 Msa1465560:intron 45.0%
GAAAAAGTGGCCAAGTTCTG+GGG - tig0045301:632-651 Msa1465560:intron 45.0%
GCAGAACTTGGGTATGTTCT+AGG - tig0045301:809-828 Msa1465560:CDS 45.0%
!! GGCTCTAAATACTGGGGTAA+AGG - tig0045301:830-849 Msa1465560:CDS 45.0%
GCTTGAAGCCTTTCCAAGTA+TGG + tig0045301:908-927 Msa1465560:intergenic 45.0%
GCTTCAAGCTCTTGTTGATG+TGG - tig0045301:919-938 Msa1465560:CDS 45.0%
! GTTGTGTTTTGCTCCATTGC+AGG - tig0045301:1309-1328 Msa1465560:CDS 45.0%
!!! TTTTTAATAAATTATTGATT+TGG + tig0045301:1277-1296 Msa1465560:intergenic 5.0%
!! AATCAATAATTTATTAAAAA+TGG - tig0045301:1277-1296 Msa1465560:CDS 5.0%
!!! TTTTTAATAAATTATTGATT+TGG + tig0045301:1277-1296 Msa1465560:intergenic 5.0%
!! AATCAATAATTTATTAAAAA+TGG - tig0045301:1277-1296 Msa1465560:CDS 5.0%
GCCAAACCATGAGATCATCG+AGG + tig0045301:604-623 Msa1465560:intergenic 50.0%
GTAAGGTTCACCCCAGAACT+TGG + tig0045301:645-664 Msa1465560:intergenic 50.0%
! CCTTGGTCTTTGCTGGTGTA+AGG + tig0045301:662-681 Msa1465560:intergenic 50.0%
CCTTACACCAGCAAAGACCA+AGG - tig0045301:659-678 Msa1465560:intron 50.0%
! GTTGATGCCTTGGTCTTTGC+TGG + tig0045301:669-688 Msa1465560:intergenic 50.0%
AGGATCGCATGTGCAGAACT+TGG - tig0045301:797-816 Msa1465560:CDS 50.0%
GGATCGCATGTGCAGAACTT+GGG - tig0045301:798-817 Msa1465560:CDS 50.0%
ACTAATGAGGGTTTGGGTCG+CGG - tig0045301:1145-1164 Msa1465560:CDS 50.0%
GCCAAACCATGAGATCATCG+AGG + tig0045301:604-623 Msa1465560:intergenic 50.0%
GTAAGGTTCACCCCAGAACT+TGG + tig0045301:645-664 Msa1465560:intergenic 50.0%
! CCTTGGTCTTTGCTGGTGTA+AGG + tig0045301:662-681 Msa1465560:intergenic 50.0%
CCTTACACCAGCAAAGACCA+AGG - tig0045301:659-678 Msa1465560:intron 50.0%
! GTTGATGCCTTGGTCTTTGC+TGG + tig0045301:669-688 Msa1465560:intergenic 50.0%
AGGATCGCATGTGCAGAACT+TGG - tig0045301:797-816 Msa1465560:CDS 50.0%
GGATCGCATGTGCAGAACTT+GGG - tig0045301:798-817 Msa1465560:CDS 50.0%
ACTAATGAGGGTTTGGGTCG+CGG - tig0045301:1145-1164 Msa1465560:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0045301 gene 497 1360 497 ID=Msa1465560;Name=Msa1465560
tig0045301 mRNA 497 1360 497 ID=Msa1465560-mRNA-1;Parent=Msa1465560;Name=Msa1465560-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|0.5|1|1|2|0|207
tig0045301 exon 767 1360 767 ID=Msa1465560-mRNA-1:exon:24820;Parent=Msa1465560-mRNA-1
tig0045301 exon 497 526 497 ID=Msa1465560-mRNA-1:exon:24819;Parent=Msa1465560-mRNA-1
tig0045301 CDS 767 1360 767 ID=Msa1465560-mRNA-1:cds;Parent=Msa1465560-mRNA-1
tig0045301 CDS 497 526 497 ID=Msa1465560-mRNA-1:cds;Parent=Msa1465560-mRNA-1
Gene Sequence

>Msa1465560

ATGGAGCAAAACACAAGTATTATGATAGAGCAACTTTCTATTTCTTCCAAAGAAGAGATCATTGATTTGAACCAAATCACACTCCGATCTTTTCATCTTTCTGACCTCGATGATCTCATGGTTTGGCATACTGATGAAAAAGTGGCCAAGTTCTGGGGTGAACCTTACACCAGCAAAGACCAAGGCATCAACTTCATTAAAAATATGGCAAGCAGGTATCTAAGTTGCAAAGCAATATGCCATAACGATCATGCTATTGGGTGTATTAAGTTGTCCTTGAGTTCACTTGATGATAAGAGGAGGATCGCATGTGCAGAACTTGGGTATGTTCTAGGCTCTAAATACTGGGGTAAAGGGATTGCCACATGTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTCAAGCTCTTGTTGATGTGGATAATTTAAGGTCGCAAAGAGTAGTTGAGAAAGTTGGTTTTCAAAAAGAGGGAGTTCTTAGGAAGTATGTATTTTTTAAAGGAAAAAGCAGAGATATGATAATGTTCAGTCTTCTTTCTACTGATCTTAAACTAATGAGGATGTTCAAGGACTTTGTTCCTTTGGTTTTGGATCCATGA

Protein sequence

>Msa1465560

MEQNTSIMIEQLSISSKEEIIDLNQITLRSFHLSDLDDLMVWHTDEKVAKFWGEPYTSKDQGINFIKNMASRYLSCKAICHNDHAIGCIKLSLSSLDDKRRIACAELGYVLGSKYWGKGIATCAVKQMVKVAFSEFPYLERLQALVDVDNLRSQRVVEKVGFQKEGVLRKYVFFKGKSRDMIMFSLLSTDLKLMRMFKDFVPLVLDP*