AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003635

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003635.t1 MTR_4g072110 98.243 569 8 2 1 567 1 569 0.0 1135
MS.gene003635.t1 MTR_3g117590 77.475 404 60 2 128 531 27 399 0.0 630
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003635.t1 AT4G34740 81.944 504 81 2 51 553 67 561 0.0 847
MS.gene003635.t1 AT2G16570 83.198 494 77 3 62 553 77 566 0.0 845
MS.gene003635.t1 AT4G38880 68.952 525 133 7 6 526 27 525 0.0 716

Find 143 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGATAGCTGTTCCTGATTC+TGG 0.178498 4.1:+31165964 MS.gene003635:CDS
GGATGAGTGTTGAAGAAATA+AGG 0.235614 4.1:+31166328 MS.gene003635:CDS
GGGTTTAGGCCTTTGGTTAT+GGG 0.237862 4.1:+31165658 MS.gene003635:CDS
TCAACAATTCTTAAAATAAA+AGG 0.259457 4.1:-31165556 None:intergenic
TGGGTTTAGGCCTTTGGTTA+TGG 0.266896 4.1:+31165657 MS.gene003635:CDS
GAGAGGATGATGTCGGAAAT+TGG 0.268968 4.1:-31165052 None:intergenic
ATTATTGGTTCTTGTTACTA+TGG 0.269970 4.1:+31166267 MS.gene003635:CDS
ATTGCAAGTCCACCAATTAT+TGG 0.272119 4.1:+31166252 MS.gene003635:CDS
TGACGATGCCGGCGCCTTCT+TGG 0.275304 4.1:-31165216 None:intergenic
GTGCTGTGCTTGAAGGTAAA+AGG 0.278979 4.1:+31166133 MS.gene003635:CDS
GTTATTGTGGTTGATAATAA+TGG 0.288801 4.1:+31165775 MS.gene003635:CDS
GTAGCAGTATTCACCTCTTT+TGG 0.290374 4.1:-31166570 None:intergenic
TGAGGGATCCGTTTGGGTTT+AGG 0.298862 4.1:+31165644 MS.gene003635:CDS
GGGAGGGAGAAGGGAGGGTT+TGG 0.307776 4.1:-31164933 None:intergenic
TCCGTTTGGGTTTAGGCCTT+TGG 0.309909 4.1:+31165651 MS.gene003635:CDS
AGAGCTAAGCTTGAAGATAA+AGG 0.329947 4.1:+31165466 MS.gene003635:CDS
TGGCCGATGGCTAAGCTTCC+AGG 0.331879 4.1:-31165322 None:intergenic
CTCTTAACCTTGAGCTCTCT+TGG 0.333802 4.1:-31166468 None:intergenic
TATGAATCGCGTAGGCGATT+TGG 0.338464 4.1:+31165901 MS.gene003635:CDS
TGATGGTTTGAATGGGAGTT+TGG 0.341680 4.1:+31166509 MS.gene003635:CDS
TTGAGCCGTCTCAGAAGATT+AGG 0.349524 4.1:+31166076 MS.gene003635:CDS
TTATTGTGGTTGATAATAAT+GGG 0.351879 4.1:+31165776 MS.gene003635:CDS
TTGAGCTCTCTTGGTTCAAC+AGG 0.352244 4.1:-31166459 None:intergenic
AGAGGATGATGTCGGAAATT+GGG 0.360260 4.1:-31165051 None:intergenic
CGCCTTCTTGGCCGCGGTGT+TGG 0.365653 4.1:-31165204 None:intergenic
TTTGTTGCTGGGTACCGTTT+TGG 0.372205 4.1:+31165394 MS.gene003635:CDS
CCGGTCCGTGCTGTGCTTGA+AGG 0.375757 4.1:+31166126 MS.gene003635:CDS
GTTGGTGACTTTGTTGATGA+TGG 0.383115 4.1:+31166492 MS.gene003635:CDS
TAGTAACAAGAACCAATAAT+TGG 0.384192 4.1:-31166264 None:intergenic
CAGAGGAGTTGATATCTAAT+AGG 0.384260 4.1:+31166307 MS.gene003635:CDS
CTAATCAACCCTTGCTGAAA+AGG 0.384647 4.1:-31166027 None:intergenic
CGTGAAGAGTGCGGAGTTGT+TGG 0.401638 4.1:+31165127 MS.gene003635:CDS
GAAGATTGTGAGGTTGCTTA+AGG 0.403149 4.1:+31166200 MS.gene003635:CDS
GGAGAAGGGAGGGTTTGGAG+AGG 0.406436 4.1:-31164928 None:intergenic
TTTGTTGATGATGGTTTGAA+TGG 0.415105 4.1:+31166501 MS.gene003635:CDS
ATTGTTGTTGGGAGGGAGAA+GGG 0.420336 4.1:-31164942 None:intergenic
CAAGAGAGCTCAAGGTTAAG+AGG 0.423035 4.1:+31166469 MS.gene003635:CDS
GTCGGAGAGAGAAGGGAGAG+TGG 0.430326 4.1:-31164993 None:intergenic
ATACTATGCAAGCTATTGAT+TGG 0.430909 4.1:-31166384 None:intergenic
GATGAGTGTTGAAGAAATAA+GGG 0.433460 4.1:+31166329 MS.gene003635:CDS
GCAGCCACAACACCAGAATC+AGG 0.436019 4.1:-31165976 None:intergenic
TTGCTGTGAGGGATCCGTTT+GGG 0.437418 4.1:+31165638 MS.gene003635:CDS
TTTGGAGGAAGTGAGGAAAA+GGG 0.443047 4.1:-31165026 None:intergenic
GGAACAAATTGATGGTGGTT+GGG 0.451957 4.1:+31166530 MS.gene003635:CDS
AATAGCTTGCATAGTATGTT+GGG 0.456007 4.1:+31166390 MS.gene003635:CDS
TCTGGTGTTGTGGCTGCGCT+TGG 0.458740 4.1:+31165982 MS.gene003635:CDS
GAGAAGGGAGGGTTTGGAGA+GGG 0.461786 4.1:-31164927 None:intergenic
CAATAGCTTGCATAGTATGT+TGG 0.465848 4.1:+31166389 MS.gene003635:CDS
GATTGTTGTTGGGAGGGAGA+AGG 0.470186 4.1:-31164943 None:intergenic
TTTGTTATGCTTGTTTCTCT+GGG 0.472388 4.1:+31166430 MS.gene003635:CDS
CTTGGTTATGCTGCGAAAGC+CGG 0.474447 4.1:+31166000 MS.gene003635:CDS
TTCTATTGTGTTTGTTACTG+AGG 0.480525 4.1:+31165606 MS.gene003635:CDS
TGCGGAGTTGTTGGAATCTA+CGG 0.482381 4.1:+31165136 MS.gene003635:CDS
GGGAGTTTGGAACAAATTGA+TGG 0.485386 4.1:+31166522 MS.gene003635:CDS
ATCACAACATCACAATCAAC+GGG 0.486896 4.1:-31165946 None:intergenic
CATGTTCGTTATTCTACTGC+TGG 0.487415 4.1:+31165343 MS.gene003635:CDS
GTTGCTGTGAGGGATCCGTT+TGG 0.488507 4.1:+31165637 MS.gene003635:CDS
TTGTTGGGAGGGAGAAGGGA+GGG 0.490395 4.1:-31164938 None:intergenic
TGCCGGCGCCTTCTTGGCCG+CGG 0.494734 4.1:-31165210 None:intergenic
GTTGAAGAAGCGATTGTTGT+TGG 0.495870 4.1:-31164954 None:intergenic
ACAAAACTTGATCAGTTACC+TGG 0.496097 4.1:+31165304 MS.gene003635:CDS
CTCCAACACCGCGGCCAAGA+AGG 0.497456 4.1:+31165202 MS.gene003635:CDS
TTGAAGAAGCGATTGTTGTT+GGG 0.499574 4.1:-31164953 None:intergenic
GGTTTAGGCCTTTGGTTATG+GGG 0.500914 4.1:+31165659 MS.gene003635:CDS
TGAGCCGTCTCAGAAGATTA+GGG 0.501845 4.1:+31166077 MS.gene003635:CDS
AGGGAGAGTGGATTTAGAGA+GGG 0.502352 4.1:-31164981 None:intergenic
AAGGGAGAGTGGATTTAGAG+AGG 0.504650 4.1:-31164982 None:intergenic
GACAAGACCAACACCGGTGA+TGG 0.504668 4.1:-31165263 None:intergenic
TGCTCAAAGATACACTGCTT+CGG 0.506723 4.1:-31165832 None:intergenic
TGGAACAAATTGATGGTGGT+TGG 0.506884 4.1:+31166529 MS.gene003635:CDS
GAGCTCAAGGTTAAGAGGGT+TGG 0.509152 4.1:+31166474 MS.gene003635:CDS
AAAGAGGTGAATACTGCTAC+TGG 0.516631 4.1:+31166573 MS.gene003635:CDS
TTGGAGGAAGTGAGGAAAAG+GGG 0.517107 4.1:-31165025 None:intergenic
GTGAGGTTGCTTAAGGAAGC+TGG 0.520822 4.1:+31166207 MS.gene003635:CDS
TTTCGGGTGTCGGAGAGAGA+AGG 0.521313 4.1:-31165001 None:intergenic
AAACGGTACCCAGCAACAAA+AGG 0.521430 4.1:-31165391 None:intergenic
TTTGTTTGAGAGGATGATGT+CGG 0.523646 4.1:-31165059 None:intergenic
CTTCAATCCATCACCGGTGT+TGG 0.525788 4.1:+31165256 MS.gene003635:CDS
TCTCAGAAGATTAGGGACTT+TGG 0.526450 4.1:+31166084 MS.gene003635:CDS
TTGTTGATGATGGTTTGAAT+GGG 0.530340 4.1:+31166502 MS.gene003635:CDS
GTTGTTGACGGTGACGATGC+CGG 0.533028 4.1:-31165227 None:intergenic
AGAAGCGATTGTTGTTGGGA+GGG 0.534074 4.1:-31164949 None:intergenic
GGAGGGCTAAGTAGCAGAGA+CGG 0.535500 4.1:-31165174 None:intergenic
TGTTCCTGATTCTGGTGTTG+TGG 0.536036 4.1:+31165972 MS.gene003635:CDS
AAGAAGCGATTGTTGTTGGG+AGG 0.538574 4.1:-31164950 None:intergenic
TGGTTCTTGTTACTATGGTG+TGG 0.539936 4.1:+31166272 MS.gene003635:CDS
GATATCAACTCCTCTGAGCT+AGG 0.540111 4.1:-31166300 None:intergenic
AAGATACACTGCTTCGGCTG+CGG 0.540981 4.1:-31165826 None:intergenic
TCAACCACAATAACCTCACC+AGG 0.542973 4.1:-31165766 None:intergenic
GAGGGCTAAGTAGCAGAGAC+GGG 0.544400 4.1:-31165173 None:intergenic
TTGAAGCACGTTGTTGTTGA+CGG 0.547527 4.1:-31165239 None:intergenic
TTTCCCTGGTGAGGTTATTG+TGG 0.547630 4.1:+31165762 MS.gene003635:CDS
CCCTTGCTGAAAAGGAACAC+CGG 0.547689 4.1:-31166019 None:intergenic
CACCGCGGCCAAGAAGGCGC+CGG 0.548864 4.1:+31165208 MS.gene003635:CDS
GGATAAGCTTGTTGCTGTGA+GGG 0.549006 4.1:+31165627 MS.gene003635:CDS
GGCCGCGGTGTTGGAGAGCG+TGG 0.550054 4.1:-31165195 None:intergenic
CGTTGATGATTCAATTGTGA+GGG 0.550589 4.1:+31166164 MS.gene003635:CDS
GTTGTTGGGAGGGAGAAGGG+AGG 0.552388 4.1:-31164939 None:intergenic
ACCAAAGGCCTAAACCCAAA+CGG 0.556825 4.1:-31165652 None:intergenic
TGCTGTGCTTGAAGGTAAAA+GGG 0.558567 4.1:+31166134 MS.gene003635:CDS
TAACAAGAACCAATAATTGG+TGG 0.560555 4.1:-31166261 None:intergenic
GCGGTGTTGGAGAGCGTGGA+GGG 0.560878 4.1:-31165191 None:intergenic
AGCCAACAGAAATCCAAAAG+AGG 0.563771 4.1:+31166557 MS.gene003635:CDS
TTCGGGTGTCGGAGAGAGAA+GGG 0.564516 4.1:-31165000 None:intergenic
TTAGGCCTTTGGTTATGGGG+AGG 0.567193 4.1:+31165662 MS.gene003635:CDS
GATGCATGTGAGAAGCTTGA+AGG 0.569398 4.1:+31165577 MS.gene003635:CDS
CAACACCCACAGAACCAAAA+CGG 0.569611 4.1:-31165408 None:intergenic
TTGATTAGGTCACACTATGT+AGG 0.570960 4.1:+31166042 MS.gene003635:CDS
CCTTCAAGCACAGCACGGAC+CGG 0.573470 4.1:-31166126 None:intergenic
TGTGAAGTTGAAGCTTTCAC+CGG 0.573769 4.1:+31166107 MS.gene003635:CDS
AAGAGAGCTCAAGGTTAAGA+GGG 0.575571 4.1:+31166470 MS.gene003635:CDS
TATCACAACATCACAATCAA+CGG 0.577052 4.1:-31165947 None:intergenic
GATTGAAGCTACTTATGAGA+GGG 0.584297 4.1:+31165735 MS.gene003635:CDS
TGATTGAAGCTACTTATGAG+AGG 0.590354 4.1:+31165734 MS.gene003635:CDS
GTTATGGGGAGGAGAAGTAA+TGG 0.595209 4.1:+31165673 MS.gene003635:CDS
TGTTGAACCAAGAGAGCTCA+AGG 0.596313 4.1:+31166461 MS.gene003635:CDS
AAACATCCTCGTCTTGTGGG+TGG 0.601035 4.1:-31165084 None:intergenic
AACGTGCTTCAATCCATCAC+CGG 0.602027 4.1:+31165250 MS.gene003635:CDS
ATCGGAGACAAGACCAACAC+CGG 0.609069 4.1:-31165269 None:intergenic
CGCGGTGTTGGAGAGCGTGG+AGG 0.613758 4.1:-31165192 None:intergenic
TCGTTGATGATTCAATTGTG+AGG 0.613921 4.1:+31166163 MS.gene003635:CDS
ACAACTCCGCACTCTTCACG+AGG 0.619515 4.1:-31165124 None:intergenic
CAGCAGTAGAATAACGAACA+TGG 0.626295 4.1:-31165342 None:intergenic
TTACCTGGAAGCTTAGCCAT+CGG 0.629983 4.1:+31165319 MS.gene003635:CDS
AGGTTGAACATTCTTCAACA+TGG 0.631085 4.1:-31165371 None:intergenic
CAACATCGTCGAAGATTGTG+AGG 0.634583 4.1:+31166190 MS.gene003635:CDS
TCTGTGGGTGTTGCTCATAA+CGG 0.634744 4.1:+31165418 MS.gene003635:CDS
AGAAGAGAGAGTTTGAGCTG+CGG 0.634768 4.1:-31164903 None:intergenic
CTTCTCCTCCCCATAACCAA+AGG 0.635089 4.1:-31165667 None:intergenic
ATCGCGTAGGCGATTTGGTG+AGG 0.637082 4.1:+31165906 MS.gene003635:CDS
AAGTCCCTAATCTTCTGAGA+CGG 0.638630 4.1:-31166081 None:intergenic
TGAGCTCTCTTGGTTCAACA+GGG 0.647432 4.1:-31166458 None:intergenic
AGTTTGGAACAAATTGATGG+TGG 0.649376 4.1:+31166525 MS.gene003635:CDS
AGGATAAGCTTGTTGCTGTG+AGG 0.658984 4.1:+31165626 MS.gene003635:CDS
GGTCTGTTTATGAATCGCGT+AGG 0.659509 4.1:+31165893 MS.gene003635:CDS
AGAATAACGAACATGGCCGA+TGG 0.666671 4.1:-31165335 None:intergenic
GTGCAAAAGAAGTTCACATG+AGG 0.670165 4.1:+31166229 MS.gene003635:CDS
GACAAACCTCGTGAAGAGTG+CGG 0.677329 4.1:+31165118 MS.gene003635:CDS
GGCTAAGTAGCAGAGACGGG+AGG 0.683072 4.1:-31165170 None:intergenic
TGTGGATACACCTAGCTCAG+AGG 0.694828 4.1:+31166290 MS.gene003635:CDS
GTTGATGATTCAATTGTGAG+GGG 0.720824 4.1:+31166165 MS.gene003635:CDS
CAACCACAATAACCTCACCA+GGG 0.721289 4.1:-31165765 None:intergenic
CTCCACGCTCTCCAACACCG+CGG 0.815634 4.1:+31165193 MS.gene003635:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAACAATTCTTAAAATAAA+AGG - chr4.1:31165559-31165578 None:intergenic 15.0%
!! AAACAAGCATAACAAAAATT+AGG - chr4.1:31166426-31166445 None:intergenic 20.0%
!!! TTATTGTGGTTGATAATAAT+GGG + chr4.1:31165776-31165795 MS.gene003635:CDS 20.0%
!!! TTTTGTTTCATTGAATACAT+CGG - chr4.1:31165290-31165309 None:intergenic 20.0%
! TAGTAACAAGAACCAATAAT+TGG - chr4.1:31166267-31166286 None:intergenic 25.0%
!! GAAGAAATAAGGGATTTTAT+TGG + chr4.1:31166339-31166358 MS.gene003635:CDS 25.0%
!!! ATTATTGGTTCTTGTTACTA+TGG + chr4.1:31166267-31166286 MS.gene003635:CDS 25.0%
!!! GTTATTGTGGTTGATAATAA+TGG + chr4.1:31165775-31165794 MS.gene003635:CDS 25.0%
ATACTATGCAAGCTATTGAT+TGG - chr4.1:31166387-31166406 None:intergenic 30.0%
CTACCAAAAACAACAGAATT+AGG - chr4.1:31165874-31165893 None:intergenic 30.0%
TAACAAGAACCAATAATTGG+TGG - chr4.1:31166264-31166283 None:intergenic 30.0%
TATCACAACATCACAATCAA+CGG - chr4.1:31165950-31165969 None:intergenic 30.0%
! AATAGCTTGCATAGTATGTT+GGG + chr4.1:31166390-31166409 MS.gene003635:CDS 30.0%
! TTCTATTGTGTTTGTTACTG+AGG + chr4.1:31165606-31165625 MS.gene003635:CDS 30.0%
! TTTGTTATGCTTGTTTCTCT+GGG + chr4.1:31166430-31166449 MS.gene003635:CDS 30.0%
!! GATGAGTGTTGAAGAAATAA+GGG + chr4.1:31166329-31166348 MS.gene003635:CDS 30.0%
!! TTGTTGATGATGGTTTGAAT+GGG + chr4.1:31166502-31166521 MS.gene003635:CDS 30.0%
!! TTTGTTGATGATGGTTTGAA+TGG + chr4.1:31166501-31166520 MS.gene003635:CDS 30.0%
!! TTTTGTTATGCTTGTTTCTC+TGG + chr4.1:31166429-31166448 MS.gene003635:CDS 30.0%
!!! CTAATTCTGTTGTTTTTGGT+AGG + chr4.1:31165872-31165891 MS.gene003635:CDS 30.0%
!!! TTGCCTAATTCTGTTGTTTT+TGG + chr4.1:31165868-31165887 MS.gene003635:CDS 30.0%
ACAAAACTTGATCAGTTACC+TGG + chr4.1:31165304-31165323 MS.gene003635:CDS 35.0%
AGAGCTAAGCTTGAAGATAA+AGG + chr4.1:31165466-31165485 MS.gene003635:CDS 35.0%
AGGTTGAACATTCTTCAACA+TGG - chr4.1:31165374-31165393 None:intergenic 35.0%
ATAAAAACATCCTCGTCTTG+TGG - chr4.1:31165091-31165110 None:intergenic 35.0%
ATCACAACATCACAATCAAC+GGG - chr4.1:31165949-31165968 None:intergenic 35.0%
ATTGCAAGTCCACCAATTAT+TGG + chr4.1:31166252-31166271 MS.gene003635:CDS 35.0%
GATTGAAGCTACTTATGAGA+GGG + chr4.1:31165735-31165754 MS.gene003635:CDS 35.0%
TAAAAACATCCTCGTCTTGT+GGG - chr4.1:31165090-31165109 None:intergenic 35.0%
TGATTGAAGCTACTTATGAG+AGG + chr4.1:31165734-31165753 MS.gene003635:CDS 35.0%
TTGATTAGGTCACACTATGT+AGG + chr4.1:31166042-31166061 MS.gene003635:CDS 35.0%
TTTGTTTGAGAGGATGATGT+CGG - chr4.1:31165062-31165081 None:intergenic 35.0%
! AATGTTCAACCTTTTGTTGC+TGG + chr4.1:31165382-31165401 MS.gene003635:CDS 35.0%
! AGTTTGGAACAAATTGATGG+TGG + chr4.1:31166525-31166544 MS.gene003635:CDS 35.0%
! ATGTTCAACCTTTTGTTGCT+GGG + chr4.1:31165383-31165402 MS.gene003635:CDS 35.0%
! CAATAGCTTGCATAGTATGT+TGG + chr4.1:31166389-31166408 MS.gene003635:CDS 35.0%
! TTGAAGAAGCGATTGTTGTT+GGG - chr4.1:31164956-31164975 None:intergenic 35.0%
!! CAGAGGAGTTGATATCTAAT+AGG + chr4.1:31166307-31166326 MS.gene003635:CDS 35.0%
!! CGTTGATGATTCAATTGTGA+GGG + chr4.1:31166164-31166183 MS.gene003635:CDS 35.0%
!! GGATGAGTGTTGAAGAAATA+AGG + chr4.1:31166328-31166347 MS.gene003635:CDS 35.0%
!! GTTGATGATTCAATTGTGAG+GGG + chr4.1:31166165-31166184 MS.gene003635:CDS 35.0%
!! TCGTTGATGATTCAATTGTG+AGG + chr4.1:31166163-31166182 MS.gene003635:CDS 35.0%
AAAGAGGTGAATACTGCTAC+TGG + chr4.1:31166573-31166592 MS.gene003635:CDS 40.0%
AAGAGAGCTCAAGGTTAAGA+GGG + chr4.1:31166470-31166489 MS.gene003635:CDS 40.0%
AGAGGATGATGTCGGAAATT+GGG - chr4.1:31165054-31165073 None:intergenic 40.0%
AGCCAACAGAAATCCAAAAG+AGG + chr4.1:31166557-31166576 MS.gene003635:CDS 40.0%
CAGCAGTAGAATAACGAACA+TGG - chr4.1:31165345-31165364 None:intergenic 40.0%
CATGTTCGTTATTCTACTGC+TGG + chr4.1:31165343-31165362 MS.gene003635:CDS 40.0%
CTAATCAACCCTTGCTGAAA+AGG - chr4.1:31166030-31166049 None:intergenic 40.0%
GAAGATTGTGAGGTTGCTTA+AGG + chr4.1:31166200-31166219 MS.gene003635:CDS 40.0%
GTGCAAAAGAAGTTCACATG+AGG + chr4.1:31166229-31166248 MS.gene003635:CDS 40.0%
GTTGAAGAAGCGATTGTTGT+TGG - chr4.1:31164957-31164976 None:intergenic 40.0%
TCTCAGAAGATTAGGGACTT+TGG + chr4.1:31166084-31166103 MS.gene003635:CDS 40.0%
TGATGGTTTGAATGGGAGTT+TGG + chr4.1:31166509-31166528 MS.gene003635:CDS 40.0%
TGCTCAAAGATACACTGCTT+CGG - chr4.1:31165835-31165854 None:intergenic 40.0%
TGCTGTGCTTGAAGGTAAAA+GGG + chr4.1:31166134-31166153 MS.gene003635:CDS 40.0%
TGGTTCTTGTTACTATGGTG+TGG + chr4.1:31166272-31166291 MS.gene003635:CDS 40.0%
TGTGAAGTTGAAGCTTTCAC+CGG + chr4.1:31166107-31166126 MS.gene003635:CDS 40.0%
TTTGGAGGAAGTGAGGAAAA+GGG - chr4.1:31165029-31165048 None:intergenic 40.0%
! AAGTCCCTAATCTTCTGAGA+CGG - chr4.1:31166084-31166103 None:intergenic 40.0%
! AAGTGAGGAAAAGGGGTTTT+CGG - chr4.1:31165021-31165040 None:intergenic 40.0%
! CACCTCTTTTGGATTTCTGT+TGG - chr4.1:31166562-31166581 None:intergenic 40.0%
! GATGTCGGAAATTGGGTTTT+TGG - chr4.1:31165047-31165066 None:intergenic 40.0%
! GGGAGTTTGGAACAAATTGA+TGG + chr4.1:31166522-31166541 MS.gene003635:CDS 40.0%
! GTAGCAGTATTCACCTCTTT+TGG - chr4.1:31166573-31166592 None:intergenic 40.0%
! TTGAAGCACGTTGTTGTTGA+CGG - chr4.1:31165242-31165261 None:intergenic 40.0%
! TTTTACCTTCAAGCACAGCA+CGG - chr4.1:31166134-31166153 None:intergenic 40.0%
!! CTTTTCAGCAAGGGTTGATT+AGG + chr4.1:31166028-31166047 MS.gene003635:CDS 40.0%
!! GGAACAAATTGATGGTGGTT+GGG + chr4.1:31166530-31166549 MS.gene003635:CDS 40.0%
!! GTTGGTGACTTTGTTGATGA+TGG + chr4.1:31166492-31166511 MS.gene003635:CDS 40.0%
!! TGGAACAAATTGATGGTGGT+TGG + chr4.1:31166529-31166548 MS.gene003635:CDS 40.0%
!! TTTTGGAGGAAGTGAGGAAA+AGG - chr4.1:31165030-31165049 None:intergenic 40.0%
AAACGGTACCCAGCAACAAA+AGG - chr4.1:31165394-31165413 None:intergenic 45.0%
AACGTGCTTCAATCCATCAC+CGG + chr4.1:31165250-31165269 MS.gene003635:CDS 45.0%
ACCAAAGGCCTAAACCCAAA+CGG - chr4.1:31165655-31165674 None:intergenic 45.0%
AGAAGAGAGAGTTTGAGCTG+CGG - chr4.1:31164906-31164925 None:intergenic 45.0%
AGAATAACGAACATGGCCGA+TGG - chr4.1:31165338-31165357 None:intergenic 45.0%
CAACACCCACAGAACCAAAA+CGG - chr4.1:31165411-31165430 None:intergenic 45.0%
CAACATCGTCGAAGATTGTG+AGG + chr4.1:31166190-31166209 MS.gene003635:CDS 45.0%
CAACCACAATAACCTCACCA+GGG - chr4.1:31165768-31165787 None:intergenic 45.0%
CAAGAGAGCTCAAGGTTAAG+AGG + chr4.1:31166469-31166488 MS.gene003635:CDS 45.0%
CTCTTAACCTTGAGCTCTCT+TGG - chr4.1:31166471-31166490 None:intergenic 45.0%
GAGAGGATGATGTCGGAAAT+TGG - chr4.1:31165055-31165074 None:intergenic 45.0%
GATATCAACTCCTCTGAGCT+AGG - chr4.1:31166303-31166322 None:intergenic 45.0%
GATGCATGTGAGAAGCTTGA+AGG + chr4.1:31165577-31165596 MS.gene003635:CDS 45.0%
GGGTTTAGGCCTTTGGTTAT+GGG + chr4.1:31165658-31165677 MS.gene003635:CDS 45.0%
GGTCTGTTTATGAATCGCGT+AGG + chr4.1:31165893-31165912 MS.gene003635:CDS 45.0%
GGTTTAGGCCTTTGGTTATG+GGG + chr4.1:31165659-31165678 MS.gene003635:CDS 45.0%
GTGATAGCTGTTCCTGATTC+TGG + chr4.1:31165964-31165983 MS.gene003635:CDS 45.0%
GTGCTGTGCTTGAAGGTAAA+AGG + chr4.1:31166133-31166152 MS.gene003635:CDS 45.0%
GTTATGGGGAGGAGAAGTAA+TGG + chr4.1:31165673-31165692 MS.gene003635:CDS 45.0%
TATGAATCGCGTAGGCGATT+TGG + chr4.1:31165901-31165920 MS.gene003635:CDS 45.0%
TCAACCACAATAACCTCACC+AGG - chr4.1:31165769-31165788 None:intergenic 45.0%
TGAGCCGTCTCAGAAGATTA+GGG + chr4.1:31166077-31166096 MS.gene003635:CDS 45.0%
TGAGCTCTCTTGGTTCAACA+GGG - chr4.1:31166461-31166480 None:intergenic 45.0%
TGCGGAGTTGTTGGAATCTA+CGG + chr4.1:31165136-31165155 MS.gene003635:CDS 45.0%
TGGGTTTAGGCCTTTGGTTA+TGG + chr4.1:31165657-31165676 MS.gene003635:CDS 45.0%
TGTTGAACCAAGAGAGCTCA+AGG + chr4.1:31166461-31166480 MS.gene003635:CDS 45.0%
TTACCTGGAAGCTTAGCCAT+CGG + chr4.1:31165319-31165338 MS.gene003635:CDS 45.0%
TTGAGCCGTCTCAGAAGATT+AGG + chr4.1:31166076-31166095 MS.gene003635:CDS 45.0%
TTGAGCTCTCTTGGTTCAAC+AGG - chr4.1:31166462-31166481 None:intergenic 45.0%
TTGGAGGAAGTGAGGAAAAG+GGG - chr4.1:31165028-31165047 None:intergenic 45.0%
TTTCCCTGGTGAGGTTATTG+TGG + chr4.1:31165762-31165781 MS.gene003635:CDS 45.0%
! AAGAAGCGATTGTTGTTGGG+AGG - chr4.1:31164953-31164972 None:intergenic 45.0%
! AAGGGAGAGTGGATTTAGAG+AGG - chr4.1:31164985-31165004 None:intergenic 45.0%
! AGAAGCGATTGTTGTTGGGA+GGG - chr4.1:31164952-31164971 None:intergenic 45.0%
! AGGGAGAGTGGATTTAGAGA+GGG - chr4.1:31164984-31165003 None:intergenic 45.0%
! AGTGAGGAAAAGGGGTTTTC+GGG - chr4.1:31165020-31165039 None:intergenic 45.0%
! ATTGTTGTTGGGAGGGAGAA+GGG - chr4.1:31164945-31164964 None:intergenic 45.0%
! TATGAGAGGGAAGTTTTCCC+TGG + chr4.1:31165748-31165767 MS.gene003635:CDS 45.0%
! TGTTCCTGATTCTGGTGTTG+TGG + chr4.1:31165972-31165991 MS.gene003635:CDS 45.0%
!! AGGATAAGCTTGTTGCTGTG+AGG + chr4.1:31165626-31165645 MS.gene003635:CDS 45.0%
!! GGATAAGCTTGTTGCTGTGA+GGG + chr4.1:31165627-31165646 MS.gene003635:CDS 45.0%
!! GTCGGAAATTGGGTTTTTGG+AGG - chr4.1:31165044-31165063 None:intergenic 45.0%
!! GTGGGTGGTTTTTGTTTGAG+AGG - chr4.1:31165072-31165091 None:intergenic 45.0%
!! TCTGTGGGTGTTGCTCATAA+CGG + chr4.1:31165418-31165437 MS.gene003635:CDS 45.0%
!! TTGGGTTTTTGGAGGAAGTG+AGG - chr4.1:31165036-31165055 None:intergenic 45.0%
!! TTTGTTGCTGGGTACCGTTT+TGG + chr4.1:31165394-31165413 MS.gene003635:CDS 45.0%
AAACATCCTCGTCTTGTGGG+TGG - chr4.1:31165087-31165106 None:intergenic 50.0%
AAGATACACTGCTTCGGCTG+CGG - chr4.1:31165829-31165848 None:intergenic 50.0%
ATCGGAGACAAGACCAACAC+CGG - chr4.1:31165272-31165291 None:intergenic 50.0%
CAAAAACCACCCACAAGACG+AGG + chr4.1:31165078-31165097 MS.gene003635:CDS 50.0%
CCCTTGCTGAAAAGGAACAC+CGG - chr4.1:31166022-31166041 None:intergenic 50.0%
CTTCAATCCATCACCGGTGT+TGG + chr4.1:31165256-31165275 MS.gene003635:CDS 50.0%
CTTCTCCTCCCCATAACCAA+AGG - chr4.1:31165670-31165689 None:intergenic 50.0%
CTTGGTTATGCTGCGAAAGC+CGG + chr4.1:31166000-31166019 MS.gene003635:CDS 50.0%
GACAAACCTCGTGAAGAGTG+CGG + chr4.1:31165118-31165137 MS.gene003635:CDS 50.0%
GAGCTCAAGGTTAAGAGGGT+TGG + chr4.1:31166474-31166493 MS.gene003635:CDS 50.0%
GTGAGGTTGCTTAAGGAAGC+TGG + chr4.1:31166207-31166226 MS.gene003635:CDS 50.0%
TGTGGATACACCTAGCTCAG+AGG + chr4.1:31166290-31166309 MS.gene003635:CDS 50.0%
TTAGGCCTTTGGTTATGGGG+AGG + chr4.1:31165662-31165681 MS.gene003635:CDS 50.0%
TTGCTGTGAGGGATCCGTTT+GGG + chr4.1:31165638-31165657 MS.gene003635:CDS 50.0%
! CCGGTGTTCCTTTTCAGCAA+GGG + chr4.1:31166019-31166038 MS.gene003635:CDS 50.0%
! GATTGTTGTTGGGAGGGAGA+AGG - chr4.1:31164946-31164965 None:intergenic 50.0%
! TCCGTTTGGGTTTAGGCCTT+TGG + chr4.1:31165651-31165670 MS.gene003635:CDS 50.0%
! TGAGGGATCCGTTTGGGTTT+AGG + chr4.1:31165644-31165663 MS.gene003635:CDS 50.0%
!!! GAAAAGGGGTTTTCGGGTGT+CGG - chr4.1:31165014-31165033 None:intergenic 50.0%
!!! GGGTACCGTTTTGGTTCTGT+GGG + chr4.1:31165403-31165422 MS.gene003635:CDS 50.0%
!!! TGGGTACCGTTTTGGTTCTG+TGG + chr4.1:31165402-31165421 MS.gene003635:CDS 50.0%
ACAACTCCGCACTCTTCACG+AGG - chr4.1:31165127-31165146 None:intergenic 55.0%
GACAAGACCAACACCGGTGA+TGG - chr4.1:31165266-31165285 None:intergenic 55.0%
GAGAAGGGAGGGTTTGGAGA+GGG - chr4.1:31164930-31164949 None:intergenic 55.0%
GAGGGCTAAGTAGCAGAGAC+GGG - chr4.1:31165176-31165195 None:intergenic 55.0%
GCAGCCACAACACCAGAATC+AGG - chr4.1:31165979-31165998 None:intergenic 55.0%
GGAGGGCTAAGTAGCAGAGA+CGG - chr4.1:31165177-31165196 None:intergenic 55.0%
GTTGCTGTGAGGGATCCGTT+TGG + chr4.1:31165637-31165656 MS.gene003635:CDS 55.0%
GTTGTTGACGGTGACGATGC+CGG - chr4.1:31165230-31165249 None:intergenic 55.0%
TTGTTGGGAGGGAGAAGGGA+GGG - chr4.1:31164941-31164960 None:intergenic 55.0%
! CGTGAAGAGTGCGGAGTTGT+TGG + chr4.1:31165127-31165146 MS.gene003635:CDS 55.0%
! GAGGGAAGTTTTCCCTGGTG+AGG + chr4.1:31165753-31165772 MS.gene003635:CDS 55.0%
! GCCGGTGTTCCTTTTCAGCA+AGG + chr4.1:31166018-31166037 MS.gene003635:CDS 55.0%
!! ATCGCGTAGGCGATTTGGTG+AGG + chr4.1:31165906-31165925 MS.gene003635:CDS 55.0%
!! TTCGGGTGTCGGAGAGAGAA+GGG - chr4.1:31165003-31165022 None:intergenic 55.0%
!! TTTCGGGTGTCGGAGAGAGA+AGG - chr4.1:31165004-31165023 None:intergenic 55.0%
CCTTCAAGCACAGCACGGAC+CGG - chr4.1:31166129-31166148 None:intergenic 60.0%
GGAGAAGGGAGGGTTTGGAG+AGG - chr4.1:31164931-31164950 None:intergenic 60.0%
GGCTAAGTAGCAGAGACGGG+AGG - chr4.1:31165173-31165192 None:intergenic 60.0%
GTCGGAGAGAGAAGGGAGAG+TGG - chr4.1:31164996-31165015 None:intergenic 60.0%
GTTGTTGGGAGGGAGAAGGG+AGG - chr4.1:31164942-31164961 None:intergenic 60.0%
TGGCCGATGGCTAAGCTTCC+AGG - chr4.1:31165325-31165344 None:intergenic 60.0%
!! TCTGGTGTTGTGGCTGCGCT+TGG + chr4.1:31165982-31166001 MS.gene003635:CDS 60.0%
CCGGTCCGTGCTGTGCTTGA+AGG + chr4.1:31166126-31166145 MS.gene003635:CDS 65.0%
CTCCAACACCGCGGCCAAGA+AGG + chr4.1:31165202-31165221 MS.gene003635:CDS 65.0%
CTCCACGCTCTCCAACACCG+CGG + chr4.1:31165193-31165212 MS.gene003635:CDS 65.0%
GGGAGGGAGAAGGGAGGGTT+TGG - chr4.1:31164936-31164955 None:intergenic 65.0%
TGACGATGCCGGCGCCTTCT+TGG - chr4.1:31165219-31165238 None:intergenic 65.0%
! GCGGTGTTGGAGAGCGTGGA+GGG - chr4.1:31165194-31165213 None:intergenic 65.0%
CGCCTTCTTGGCCGCGGTGT+TGG - chr4.1:31165207-31165226 None:intergenic 70.0%
! CGCGGTGTTGGAGAGCGTGG+AGG - chr4.1:31165195-31165214 None:intergenic 70.0%
TGCCGGCGCCTTCTTGGCCG+CGG - chr4.1:31165213-31165232 None:intergenic 75.0%
! CACCGCGGCCAAGAAGGCGC+CGG + chr4.1:31165208-31165227 MS.gene003635:CDS 75.0%
! GGCCGCGGTGTTGGAGAGCG+TGG - chr4.1:31165198-31165217 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31164899 31166602 31164899 ID=MS.gene003635
chr4.1 mRNA 31164899 31166602 31164899 ID=MS.gene003635.t1;Parent=MS.gene003635
chr4.1 exon 31164899 31166602 31164899 ID=MS.gene003635.t1.exon1;Parent=MS.gene003635.t1
chr4.1 CDS 31164899 31166602 31164899 ID=cds.MS.gene003635.t1;Parent=MS.gene003635.t1
Gene Sequence

>MS.gene003635

ATGGCCGCAGCTCAAACTCTCTCTTCTTCCCTCTCCAAACCCTCCCTTCTCCCTCCCAACAACAATCGCTTCTTCAACACATCCCTCTCTAAATCCACTCTCCCTTCTCTCTCCGACACCCGAAAACCCCTTTTCCTCACTTCCTCCAAAAACCCAATTTCCGACATCATCCTCTCAAACAAAAACCACCCACAAGACGAGGATGTTTTTATCAACGACGACAAACCTCGTGAAGAGTGCGGAGTTGTTGGAATCTACGGCGACTCAGAAGCCTCCCGTCTCTGCTACTTAGCCCTCCACGCTCTCCAACACCGCGGCCAAGAAGGCGCCGGCATCGTCACCGTCAACAACAACGTGCTTCAATCCATCACCGGTGTTGGTCTTGTCTCCGATGTATTCAATGAAACAAAACTTGATCAGTTACCTGGAAGCTTAGCCATCGGCCATGTTCGTTATTCTACTGCTGGACAGTCCATGTTGAAGAATGTTCAACCTTTTGTTGCTGGGTACCGTTTTGGTTCTGTGGGTGTTGCTCATAACGGTAATTTAGTTAATTATCGTACTTTAAGAGCTAAGCTTGAAGATAAAGGTTCGATTTTTAACACTACTTCTGATACTGAAGTTGTTCTTCATTTGATTGCTACTTCAAAGCATAGACCTTTTATTTTAAGAATTGTTGATGCATGTGAGAAGCTTGAAGGTGCTTATTCTATTGTGTTTGTTACTGAGGATAAGCTTGTTGCTGTGAGGGATCCGTTTGGGTTTAGGCCTTTGGTTATGGGGAGGAGAAGTAATGGTGCTGTTGTTTTTGCTTCTGAGACTTGTGCTCTTGATTTGATTGAAGCTACTTATGAGAGGGAAGTTTTCCCTGGTGAGGTTATTGTGGTTGATAATAATGGGATTCAATCGCTCTGTTTGATGTCGCATCCGCAGCCGAAGCAGTGTATCTTTGAGCATATTTACTTTGCTTTGCCTAATTCTGTTGTTTTTGGTAGGTCTGTTTATGAATCGCGTAGGCGATTTGGTGAGGTGCTTGCTACTGAAAGTCCCGTTGATTGTGATGTTGTGATAGCTGTTCCTGATTCTGGTGTTGTGGCTGCGCTTGGTTATGCTGCGAAAGCCGGTGTTCCTTTTCAGCAAGGGTTGATTAGGTCACACTATGTAGGAAGAACTTTCATTGAGCCGTCTCAGAAGATTAGGGACTTTGGTGTGAAGTTGAAGCTTTCACCGGTCCGTGCTGTGCTTGAAGGTAAAAGGGTTGTTGTCGTTGATGATTCAATTGTGAGGGGAACAACATCGTCGAAGATTGTGAGGTTGCTTAAGGAAGCTGGTGCAAAAGAAGTTCACATGAGGATTGCAAGTCCACCAATTATTGGTTCTTGTTACTATGGTGTGGATACACCTAGCTCAGAGGAGTTGATATCTAATAGGATGAGTGTTGAAGAAATAAGGGATTTTATTGGATCTGATTCACTTGCTTTTCTTCCAATCAATAGCTTGCATAGTATGTTGGGTAATGATTCTCCTAATTTTTGTTATGCTTGTTTCTCTGGGAAATACCCTGTTGAACCAAGAGAGCTCAAGGTTAAGAGGGTTGGTGACTTTGTTGATGATGGTTTGAATGGGAGTTTGGAACAAATTGATGGTGGTTGGGTTCAAGCCAACAGAAATCCAAAAGAGGTGAATACTGCTACTGGTCTTTGA
Protein sequence

>MS.gene003635.t1

MAAAQTLSSSLSKPSLLPPNNNRFFNTSLSKSTLPSLSDTRKPLFLTSSKNPISDIILSNKNHPQDEDVFINDDKPREECGVVGIYGDSEASRLCYLALHALQHRGQEGAGIVTVNNNVLQSITGVGLVSDVFNETKLDQLPGSLAIGHVRYSTAGQSMLKNVQPFVAGYRFGSVGVAHNGNLVNYRTLRAKLEDKGSIFNTTSDTEVVLHLIATSKHRPFILRIVDACEKLEGAYSIVFVTEDKLVAVRDPFGFRPLVMGRRSNGAVVFASETCALDLIEATYEREVFPGEVIVVDNNGIQSLCLMSHPQPKQCIFEHIYFALPNSVVFGRSVYESRRRFGEVLATESPVDCDVVIAVPDSGVVAALGYAAKAGVPFQQGLIRSHYVGRTFIEPSQKIRDFGVKLKLSPVRAVLEGKRVVVVDDSIVRGTTSSKIVRLLKEAGAKEVHMRIASPPIIGSCYYGVDTPSSEELISNRMSVEEIRDFIGSDSLAFLPINSLHSMLGNDSPNFCYACFSGKYPVEPRELKVKRVGDFVDDGLNGSLEQIDGGWVQANRNPKEVNTATGL