Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004964.t1 | XP_013468859.1 | 97.8 | 368 | 8 | 0 | 123 | 490 | 100 | 467 | 2.40E-152 | 548.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004964.t1 | Q9M0N1 | 77.7 | 332 | 73 | 1 | 159 | 489 | 149 | 480 | 7.4e-102 | 372.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004964.t1 | A0A072VMS3 | 97.8 | 368 | 8 | 0 | 123 | 490 | 100 | 467 | 1.7e-152 | 548.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene004964.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004964 | MS.gene051468 | PPI |
| MS.gene004964 | MS.gene035055 | PPI |
| MS.gene064475 | MS.gene004964 | PPI |
| MS.gene004964 | MS.gene029804 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004964.t1 | MTR_1g077740 | 98.370 | 368 | 6 | 0 | 123 | 490 | 100 | 467 | 0.0 | 731 |
| MS.gene004964.t1 | MTR_4g105430 | 37.500 | 360 | 199 | 7 | 143 | 487 | 22 | 370 | 8.11e-72 | 233 |
| MS.gene004964.t1 | MTR_1g077700 | 71.000 | 100 | 25 | 2 | 1 | 96 | 1 | 100 | 8.36e-37 | 134 |
| MS.gene004964.t1 | MTR_1g077730 | 71.264 | 87 | 7 | 3 | 1 | 86 | 1 | 70 | 1.51e-29 | 115 |
| MS.gene004964.t1 | MTR_4g102810 | 36.667 | 180 | 91 | 5 | 311 | 488 | 421 | 579 | 1.08e-13 | 73.9 |
| MS.gene004964.t1 | MTR_8g432670 | 30.625 | 160 | 96 | 3 | 330 | 488 | 354 | 499 | 9.91e-13 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004964.t1 | AT4G10790 | 73.913 | 368 | 92 | 2 | 123 | 489 | 116 | 480 | 0.0 | 520 |
| MS.gene004964.t1 | AT4G23040 | 34.225 | 187 | 104 | 4 | 304 | 488 | 355 | 524 | 4.17e-14 | 75.1 |
| MS.gene004964.t1 | AT4G11740 | 32.222 | 180 | 110 | 3 | 311 | 488 | 394 | 563 | 1.99e-12 | 69.7 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTCCCTCGGGTTGTTTA+TGG | 0.181565 | 1.4:+63683348 | MS.gene004964:CDS |
| ACTCACAAAATTCAGCTTAA+AGG | 0.197742 | 1.4:-63676974 | None:intergenic |
| ATTCACTTTGGTGACATTAA+TGG | 0.242417 | 1.4:-63668834 | None:intergenic |
| ATCTCAGGAGCAATCGGTTT+TGG | 0.260770 | 1.4:+63676778 | MS.gene004964:CDS |
| CTACAAAACTCCGGTGTATC+AGG | 0.293692 | 1.4:-63677075 | None:intergenic |
| AGCCTTGCTGCAACAAGAAC+TGG | 0.308918 | 1.4:-63682531 | None:intergenic |
| CTTGGTGAAGAACCTGAAAA+AGG | 0.335458 | 1.4:+63682964 | MS.gene004964:CDS |
| CTTTCCTTGCGCACACCATT+GGG | 0.343968 | 1.4:-63683234 | None:intergenic |
| CCTTTCCTTGCGCACACCAT+TGG | 0.365652 | 1.4:-63683235 | None:intergenic |
| GTATGACTATGTTGATTCAT+TGG | 0.383014 | 1.4:+63683290 | MS.gene004964:CDS |
| TACTTTACCTGTGTAACATT+AGG | 0.391398 | 1.4:-63682988 | None:intergenic |
| GTGGCGGCTGACGATGAGTT+TGG | 0.397273 | 1.4:-63676856 | None:intergenic |
| TATGACTATGTTGATTCATT+GGG | 0.400057 | 1.4:+63683291 | MS.gene004964:CDS |
| CTTGAGCTTTCTCTCAGCTT+CGG | 0.405704 | 1.4:-63682851 | None:intergenic |
| GAGAATCGCGCTTCTTCAAC+AGG | 0.409834 | 1.4:+63677254 | MS.gene004964:CDS |
| TATTACGTACGACGTCTGTT+AGG | 0.410217 | 1.4:-63674570 | None:intergenic |
| ACGAACTAATGCACGCTTAA+GGG | 0.410986 | 1.4:+63682569 | MS.gene004964:CDS |
| TCCTCAGGCCAGTCTGTTTG+TGG | 0.421845 | 1.4:+63683413 | MS.gene004964:CDS |
| GACGAACTAATGCACGCTTA+AGG | 0.429809 | 1.4:+63682568 | MS.gene004964:CDS |
| GAATAAGCAATGATGGAGAA+AGG | 0.431980 | 1.4:-63668896 | None:intergenic |
| TTCACTTCATTTCAAGCACT+TGG | 0.432350 | 1.4:+63668992 | MS.gene004964:CDS |
| TAAGATGAGTAATAGTTTGA+AGG | 0.444349 | 1.4:+63677185 | MS.gene004964:CDS |
| TTCCTCTGCTGGCACGCCGT+TGG | 0.444655 | 1.4:+63676879 | MS.gene004964:CDS |
| GCCTAGTCTCTAACTTCCCT+CGG | 0.451100 | 1.4:+63683337 | MS.gene004964:CDS |
| TCCGGTGTATCAGGATGATC+AGG | 0.452932 | 1.4:-63677066 | None:intergenic |
| TTGTAGAAGGACGCTTTGTT+CGG | 0.456059 | 1.4:+63677092 | MS.gene004964:CDS |
| TCGCTCGGCTTCGTCGGCCT+CGG | 0.460895 | 1.4:+63676832 | MS.gene004964:CDS |
| GGCTCGTGAAAGAGCAGCAC+AGG | 0.468262 | 1.4:+63682885 | MS.gene004964:CDS |
| TCAGCCGCCACTTCCTCTGC+TGG | 0.471420 | 1.4:+63676868 | MS.gene004964:CDS |
| CCTAGAAAAGTTCATAGAAA+AGG | 0.473509 | 1.4:+63669040 | MS.gene004964:CDS |
| CAAAGTGAATATAACTCAAA+AGG | 0.474354 | 1.4:+63668847 | MS.gene004964:CDS |
| AAGAGCAAGATGCTGCATAC+AGG | 0.474369 | 1.4:+63682592 | MS.gene004964:CDS |
| GCCTTGCTGCAACAAGAACT+GGG | 0.476016 | 1.4:-63682530 | None:intergenic |
| AGTAGAAGAATAAGCAATGA+TGG | 0.477070 | 1.4:-63668903 | None:intergenic |
| CGGCGTGCCAGCAGAGGAAG+TGG | 0.477757 | 1.4:-63676875 | None:intergenic |
| CCCCAGTTCTTGTTGCAGCA+AGG | 0.480156 | 1.4:+63682529 | MS.gene004964:CDS |
| GGGTTAATCTCAGGAGCAAT+CGG | 0.482162 | 1.4:+63676772 | MS.gene004964:intron |
| AATAAGCAATGATGGAGAAA+GGG | 0.498901 | 1.4:-63668895 | None:intergenic |
| AAAATTGGCACTCTCACTAA+AGG | 0.514236 | 1.4:+63683377 | MS.gene004964:CDS |
| TTTGAGAGGGATTACGGTGG+TGG | 0.515248 | 1.4:+63676949 | MS.gene004964:CDS |
| GAAGAGAAAGCTCAATCTCT+TGG | 0.515787 | 1.4:+63682946 | MS.gene004964:CDS |
| GGGAATATTCGTGCTAGTGA+AGG | 0.517235 | 1.4:+63677159 | MS.gene004964:CDS |
| AGAAAGGGATAGGAGATTGT+GGG | 0.519333 | 1.4:-63668880 | None:intergenic |
| ACTTCATTTCAAGCACTTGG+AGG | 0.520190 | 1.4:+63668995 | MS.gene004964:CDS |
| AGTGTCACTAACATCTCCTC+GGG | 0.521835 | 1.4:-63682483 | None:intergenic |
| GCTTATTCTTCTACTAGAGA+AGG | 0.521996 | 1.4:+63668911 | MS.gene004964:CDS |
| GCAATGATGGAGAAAGGGAT+AGG | 0.524368 | 1.4:-63668890 | None:intergenic |
| GCCGACGAAGCCGAGCGAAT+AGG | 0.525334 | 1.4:-63676827 | None:intergenic |
| GCACTCTCACTAAAGGAAGC+TGG | 0.528422 | 1.4:+63683384 | MS.gene004964:CDS |
| TCCTGATCATCCTGATACAC+CGG | 0.529879 | 1.4:+63677065 | MS.gene004964:CDS |
| TCCTATTCGCTCGGCTTCGT+CGG | 0.530410 | 1.4:+63676826 | MS.gene004964:CDS |
| GACGTCTGTTAGGAATCATC+TGG | 0.530880 | 1.4:-63674560 | None:intergenic |
| AAGAGGAAGAACGTCTTGCA+AGG | 0.535723 | 1.4:+63682821 | MS.gene004964:CDS |
| GAGAAAGGGATAGGAGATTG+TGG | 0.535781 | 1.4:-63668881 | None:intergenic |
| GGAAGCTGGATTGCATCCTC+AGG | 0.540277 | 1.4:+63683398 | MS.gene004964:CDS |
| AAAGGGATAGGAGATTGTGG+GGG | 0.541180 | 1.4:-63668878 | None:intergenic |
| ATGACTACGGAGAGAAAGAA+AGG | 0.542698 | 1.4:+63669794 | MS.gene004964:CDS |
| AAGTGTCACTAACATCTCCT+CGG | 0.548419 | 1.4:-63682484 | None:intergenic |
| GCCATAACAAGAGCACAAAA+CGG | 0.551788 | 1.4:-63677219 | None:intergenic |
| GGTGTTCTATCCTATTCGCT+CGG | 0.554220 | 1.4:+63676817 | MS.gene004964:CDS |
| GGAATATTCGTGCTAGTGAA+GGG | 0.556663 | 1.4:+63677160 | MS.gene004964:CDS |
| AACCAACGGCGTGCCAGCAG+AGG | 0.558001 | 1.4:-63676881 | None:intergenic |
| TAGAAGGACGCTTTGTTCGG+AGG | 0.560780 | 1.4:+63677095 | MS.gene004964:CDS |
| GTGATGAAATATTATGACTA+CGG | 0.561623 | 1.4:+63669781 | MS.gene004964:CDS |
| GAAAGGGATAGGAGATTGTG+GGG | 0.561722 | 1.4:-63668879 | None:intergenic |
| TTTGTGGAGCTTAGTTCATG+AGG | 0.563439 | 1.4:+63683429 | MS.gene004964:CDS |
| CACAATTATAGGTGTTGGTG+CGG | 0.563768 | 1.4:+63683208 | MS.gene004964:intron |
| CCAATGGTGTGCGCAAGGAA+AGG | 0.565379 | 1.4:+63683235 | MS.gene004964:CDS |
| CGAAGCTGAGAGAAAGCTCA+AGG | 0.565583 | 1.4:+63682852 | MS.gene004964:CDS |
| CCTAGTCTCTAACTTCCCTC+GGG | 0.569165 | 1.4:+63683338 | MS.gene004964:CDS |
| GTTTCCCAATGGTGTGCGCA+AGG | 0.577538 | 1.4:+63683230 | MS.gene004964:CDS |
| GGTAGTCTAGGGTTAATCTC+AGG | 0.589893 | 1.4:+63676763 | MS.gene004964:intron |
| ACTAAGCTCCACAAACAGAC+TGG | 0.594516 | 1.4:-63683421 | None:intergenic |
| GAGAAAGCTCAAGGAAGAAG+AGG | 0.595361 | 1.4:+63682861 | MS.gene004964:CDS |
| AAGGGATAGGAGATTGTGGG+GGG | 0.595859 | 1.4:-63668877 | None:intergenic |
| TGGATGCGTTACAGAGGTCA+AGG | 0.599363 | 1.4:+63677007 | MS.gene004964:CDS |
| AGAGCAAGATGCTGCATACA+GGG | 0.607490 | 1.4:+63682593 | MS.gene004964:CDS |
| CCCGAGGGAAGTTAGAGACT+AGG | 0.610507 | 1.4:-63683338 | None:intergenic |
| AGAGGAAGAACGTCTTGCAA+GGG | 0.610541 | 1.4:+63682822 | MS.gene004964:CDS |
| AGCGTCCTTCTACAAAACTC+CGG | 0.619964 | 1.4:-63677084 | None:intergenic |
| GGAACGGCAGAGAAGAGAAG+AGG | 0.621468 | 1.4:+63682804 | MS.gene004964:CDS |
| ATGGTGTGCGCAAGGAAAGG+AGG | 0.621807 | 1.4:+63683238 | MS.gene004964:CDS |
| TGTATGTCCAGGCTAGGGAA+CGG | 0.623782 | 1.4:+63682788 | MS.gene004964:intron |
| GTGTTGGTGCGGTTTCCCAA+TGG | 0.626912 | 1.4:+63683219 | MS.gene004964:CDS |
| AAAGCTCAAGGAAGAAGAGG+AGG | 0.627771 | 1.4:+63682864 | MS.gene004964:CDS |
| TATTCTTCTACTAGAGAAGG+AGG | 0.628197 | 1.4:+63668914 | MS.gene004964:CDS |
| TTTGGATTATGGGCTGCCGG+TGG | 0.634831 | 1.4:+63676796 | MS.gene004964:CDS |
| CGAATAGGATAGAACACCAC+CGG | 0.640885 | 1.4:-63676812 | None:intergenic |
| AAAAGGACCTAATGTTACAC+AGG | 0.641329 | 1.4:+63682981 | MS.gene004964:CDS |
| CGTGCCAGCAGAGGAAGTGG+CGG | 0.642187 | 1.4:-63676872 | None:intergenic |
| TCTTGTCCATAAACAACCCG+AGG | 0.649081 | 1.4:-63683354 | None:intergenic |
| GTTTCATGGATGCGTTACAG+AGG | 0.661702 | 1.4:+63677001 | MS.gene004964:CDS |
| TCCACAAACAGACTGGCCTG+AGG | 0.673911 | 1.4:-63683414 | None:intergenic |
| GTAATAGTTTGAAGGCGTCG+AGG | 0.675844 | 1.4:+63677193 | MS.gene004964:CDS |
| GTGTCACTAACATCTCCTCG+GGG | 0.689574 | 1.4:-63682482 | None:intergenic |
| CTTGTCCATAAACAACCCGA+GGG | 0.695556 | 1.4:-63683353 | None:intergenic |
| GCAGCAGAAACAGAAACCAA+CGG | 0.700084 | 1.4:-63676895 | None:intergenic |
| CCTTGCTGCAACAAGAACTG+GGG | 0.706366 | 1.4:-63682529 | None:intergenic |
| CTGACGATGAGTTTGGACCG+AGG | 0.729107 | 1.4:-63676849 | None:intergenic |
| TGTCACTAACATCTCCTCGG+GGG | 0.758406 | 1.4:-63682481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 63668788 | 63683449 | 63668788 | ID=MS.gene004964 |
| chr1.4 | mRNA | 63668788 | 63683449 | 63668788 | ID=MS.gene004964.t1;Parent=MS.gene004964 |
| chr1.4 | exon | 63668788 | 63669061 | 63668788 | ID=MS.gene004964.t1.exon1;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63668788 | 63669061 | 63668788 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| chr1.4 | exon | 63669776 | 63669817 | 63669776 | ID=MS.gene004964.t1.exon2;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63669776 | 63669817 | 63669776 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| chr1.4 | exon | 63674535 | 63674582 | 63674535 | ID=MS.gene004964.t1.exon3;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63674535 | 63674582 | 63674535 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| chr1.4 | exon | 63676773 | 63677275 | 63676773 | ID=MS.gene004964.t1.exon4;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63676773 | 63677275 | 63676773 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| chr1.4 | exon | 63682465 | 63682635 | 63682465 | ID=MS.gene004964.t1.exon5;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63682465 | 63682635 | 63682465 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| chr1.4 | exon | 63682799 | 63683002 | 63682799 | ID=MS.gene004964.t1.exon6;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63682799 | 63683002 | 63682799 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| chr1.4 | exon | 63683219 | 63683449 | 63683219 | ID=MS.gene004964.t1.exon7;Parent=MS.gene004964.t1 |
| chr1.4 | CDS | 63683219 | 63683449 | 63683219 | ID=cds.MS.gene004964.t1;Parent=MS.gene004964.t1 |
| Gene Sequence |
| Protein sequence |
