Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005326.t1 | XP_013469076.1 | 98.6 | 484 | 7 | 0 | 1 | 484 | 1 | 484 | 8.40E-283 | 982.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005326.t1 | F4HZG9 | 68.7 | 469 | 144 | 2 | 1 | 469 | 1 | 466 | 2.8e-194 | 679.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005326.t1 | A0A072VM73 | 98.6 | 484 | 7 | 0 | 1 | 484 | 1 | 484 | 6.0e-283 | 982.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene005326 | MS.gene02160 | PPI |
| MS.gene048313 | MS.gene005326 | PPI |
| MS.gene005326 | MS.gene02843 | PPI |
| MS.gene005326 | MS.gene003186 | PPI |
| MS.gene006974 | MS.gene005326 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005326.t1 | MTR_1g084190 | 98.554 | 484 | 7 | 0 | 1 | 484 | 1 | 484 | 0.0 | 998 |
| MS.gene005326.t1 | MTR_8g101730 | 26.582 | 474 | 285 | 16 | 51 | 464 | 8 | 478 | 1.93e-37 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005326.t1 | AT1G09300 | 68.657 | 469 | 144 | 2 | 1 | 469 | 1 | 466 | 0.0 | 691 |
| MS.gene005326.t1 | AT1G09300 | 70.794 | 428 | 123 | 1 | 42 | 469 | 10 | 435 | 0.0 | 654 |
| MS.gene005326.t1 | AT4G29490 | 25.176 | 425 | 259 | 14 | 93 | 464 | 59 | 477 | 2.35e-37 | 143 |
Find 130 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGGTGGTGTGGCCATATT+AGG | 0.133457 | 1.1:+62480199 | MS.gene005326:CDS |
| ATGTAGCTCTCAAGTTATTT+TGG | 0.237616 | 1.1:-62488201 | None:intergenic |
| CTCACATAAGGTTTGGCTTC+TGG | 0.259772 | 1.1:-62480250 | None:intergenic |
| TGAATTTGCTTCACTTCTTT+CGG | 0.281191 | 1.1:-62488136 | None:intergenic |
| ACCAATGTCATGCCCTAATA+TGG | 0.296281 | 1.1:-62480212 | None:intergenic |
| GAGGAACCCCTTACCATATC+TGG | 0.297990 | 1.1:-62481383 | None:intergenic |
| TAGAAGTTGGGTTCAGCTTA+TGG | 0.298215 | 1.1:-62484323 | None:intergenic |
| ACATCTGTCATCATCTTTAC+TGG | 0.311255 | 1.1:-62480118 | None:intergenic |
| AATTAGTATTTGCAGGTTCT+AGG | 0.324370 | 1.1:+62479028 | MS.gene005326:intron |
| GCCGATTACTCATATATTAC+AGG | 0.336669 | 1.1:+62480166 | MS.gene005326:CDS |
| ACAACTTCAGCCATGGAATA+GGG | 0.337029 | 1.1:+62488170 | MS.gene005326:CDS |
| ACCTCGTTCAACTTTCTCAT+TGG | 0.343552 | 1.1:-62481140 | None:intergenic |
| TATTCAGATCCTTCCAGATA+TGG | 0.348584 | 1.1:+62481370 | MS.gene005326:intron |
| GCATCTTGTCGAAATGTATA+AGG | 0.350875 | 1.1:-62480145 | None:intergenic |
| ATTGATCCAGGTCACTATCT+AGG | 0.354401 | 1.1:+62484470 | MS.gene005326:intron |
| GACTGCTACATCAGCATATA+CGG | 0.360739 | 1.1:+62481427 | MS.gene005326:CDS |
| AATTTGTCTAGGATGTGATT+TGG | 0.363924 | 1.1:+62481057 | MS.gene005326:intron |
| CTTTCGGAATTGATGCTGTT+AGG | 0.367090 | 1.1:-62488120 | None:intergenic |
| GAAGTGTTATACATTATTCT+AGG | 0.368162 | 1.1:+62482082 | MS.gene005326:CDS |
| ATCTGCATCAATTGCTTGTC+AGG | 0.376334 | 1.1:+62481562 | MS.gene005326:CDS |
| ATGATAGCCAAACTCTTCTC+TGG | 0.383373 | 1.1:-62480085 | None:intergenic |
| GAGATTCTCATTACAGAAAC+AGG | 0.389989 | 1.1:+62487061 | MS.gene005326:CDS |
| CTGTTTGTGTGAAAATGTTT+TGG | 0.397576 | 1.1:-62478902 | None:intergenic |
| TTCAATCCCGTGGTTGGTGG+TGG | 0.398757 | 1.1:+62482051 | MS.gene005326:CDS |
| CATGTTTGGCAGATTAAAGA+TGG | 0.406377 | 1.1:+62482176 | MS.gene005326:intron |
| AAGCTGAACCCAACTTCTAT+AGG | 0.407794 | 1.1:+62484327 | MS.gene005326:CDS |
| ATGAGAGGTGCGCAGCGAAT+GGG | 0.410718 | 1.1:+62481820 | MS.gene005326:CDS |
| TCTGGACAATTAAAAGAAGA+TGG | 0.413497 | 1.1:-62485588 | None:intergenic |
| AACAACTTCAGCCATGGAAT+AGG | 0.413497 | 1.1:+62488169 | MS.gene005326:CDS |
| GTGGTTGGTGGTGGGCCTAA+TGG | 0.419819 | 1.1:+62482060 | MS.gene005326:CDS |
| ACAGTAAGCTAGTTTCTTGA+AGG | 0.421447 | 1.1:-62481459 | None:intergenic |
| GCCTGTAATATATGAGTAAT+CGG | 0.422629 | 1.1:-62480167 | None:intergenic |
| AGTACCTGCGGGTGAGATGC+AGG | 0.425788 | 1.1:-62479103 | None:intergenic |
| GCAGCTAGCATACCTTCAAA+AGG | 0.428825 | 1.1:-62481775 | None:intergenic |
| GCATTGTAGCTTCTAAAAGA+TGG | 0.435409 | 1.1:+62479995 | MS.gene005326:intron |
| TATCAGACTCACATAAGGTT+TGG | 0.437944 | 1.1:-62480257 | None:intergenic |
| GCTTCTCCCAGAGAAGAGTT+TGG | 0.441988 | 1.1:+62480078 | MS.gene005326:CDS |
| CACAAGGTTTCTGTTGATGT+TGG | 0.444626 | 1.1:+62479070 | MS.gene005326:CDS |
| CATTACAGAAACAGGTTATG+AGG | 0.448108 | 1.1:+62487069 | MS.gene005326:CDS |
| CATCTGTCATCATCTTTACT+GGG | 0.449826 | 1.1:-62480117 | None:intergenic |
| AACTTTAGTAAGTACCTCTC+TGG | 0.452530 | 1.1:-62485606 | None:intergenic |
| GGCTATCAACCCCTATTCCA+TGG | 0.458885 | 1.1:-62488180 | None:intergenic |
| CATTGTAGCTTCTAAAAGAT+GGG | 0.460019 | 1.1:+62479996 | MS.gene005326:intron |
| TGGTAGCTTCTCTTCTGCCC+AGG | 0.468113 | 1.1:+62482274 | MS.gene005326:CDS |
| GGTACCAAGGCATTGGGATA+AGG | 0.470535 | 1.1:+62487029 | MS.gene005326:intron |
| CCGAATGGTTGTGTATTTGT+CGG | 0.470570 | 1.1:-62483273 | None:intergenic |
| CTGGTGGTGTGGCCATATTA+GGG | 0.470726 | 1.1:+62480200 | MS.gene005326:CDS |
| TGCAGCATTGAATACATTCA+AGG | 0.471710 | 1.1:+62481103 | MS.gene005326:CDS |
| CCTAATATGGCCACACCACC+AGG | 0.477889 | 1.1:-62480199 | None:intergenic |
| TAATGCAGGTACCAAGGCAT+TGG | 0.480015 | 1.1:+62487022 | MS.gene005326:intron |
| TTCATCAGCTTGAGCTCTGA+AGG | 0.481437 | 1.1:-62481536 | None:intergenic |
| TGTGTGGAACTTTGTAAGCC+TGG | 0.488945 | 1.1:+62483241 | MS.gene005326:CDS |
| TTTGACTGTCCTCTGAAGCC+AGG | 0.489111 | 1.1:+62484524 | MS.gene005326:CDS |
| ACCAGGCTACCTATAGAAGT+TGG | 0.492131 | 1.1:-62484336 | None:intergenic |
| AGATCCTTCCAGATATGGTA+AGG | 0.492996 | 1.1:+62481375 | MS.gene005326:intron |
| GTCTAGGATGTGATTTGGCA+AGG | 0.493497 | 1.1:+62481062 | MS.gene005326:intron |
| AACATTGTGATACAATTTCG+AGG | 0.498748 | 1.1:-62481402 | None:intergenic |
| AATGAGAGGTGCGCAGCGAA+TGG | 0.501064 | 1.1:+62481819 | MS.gene005326:CDS |
| TAATGTATAACACTTCCATT+AGG | 0.503380 | 1.1:-62482075 | None:intergenic |
| TGGCCACACCACCAGGTTGT+TGG | 0.503866 | 1.1:-62480192 | None:intergenic |
| TTCAGAGCTCAAGCTGATGA+AGG | 0.504671 | 1.1:+62481538 | MS.gene005326:CDS |
| ATTTGGCAAGGGCATGTAGC+AGG | 0.507631 | 1.1:+62481074 | MS.gene005326:CDS |
| TCAGCGATCTCACGCGTACC+TGG | 0.508919 | 1.1:+62482243 | MS.gene005326:CDS |
| CAGAAAGGACTCAAGGAGGT+TGG | 0.513664 | 1.1:+62484270 | MS.gene005326:CDS |
| AGATTCAATCCCGTGGTTGG+TGG | 0.514269 | 1.1:+62482048 | MS.gene005326:intron |
| ATCAACAGAAACCTTGTGAC+TGG | 0.514653 | 1.1:-62479065 | None:intergenic |
| AATGCAGGTACCAAGGCATT+GGG | 0.515702 | 1.1:+62487023 | MS.gene005326:intron |
| CTGGACAATTAAAAGAAGAT+GGG | 0.518907 | 1.1:-62485587 | None:intergenic |
| TACATCAGCATATACGGAAC+TGG | 0.519360 | 1.1:+62481433 | MS.gene005326:CDS |
| TGAAGGTATGCTAGCTGCAA+AGG | 0.521850 | 1.1:+62481780 | MS.gene005326:CDS |
| GAAGCTACCACAAGGTGGCC+AGG | 0.525969 | 1.1:-62482261 | None:intergenic |
| CCAGGCTACCTATAGAAGTT+GGG | 0.533663 | 1.1:-62484335 | None:intergenic |
| CTTACAACACCTGGCTTCAG+AGG | 0.541034 | 1.1:-62484533 | None:intergenic |
| CAGCTAGCATACCTTCAAAA+GGG | 0.544862 | 1.1:-62481774 | None:intergenic |
| TGCACATGGACAGTATCAGC+TGG | 0.550753 | 1.1:-62488224 | None:intergenic |
| TTTATACCCATCAGCTGCGA+TGG | 0.553980 | 1.1:+62481504 | MS.gene005326:CDS |
| TATTCCTCACTAGATATGCC+TGG | 0.555430 | 1.1:-62480028 | None:intergenic |
| TGCGGGTGAGATGCAGGGGT+TGG | 0.558374 | 1.1:-62479097 | None:intergenic |
| TCTAGGATGTGATTTGGCAA+GGG | 0.563003 | 1.1:+62481063 | MS.gene005326:intron |
| AGGCATTGGGATAAGGATTG+AGG | 0.564277 | 1.1:+62487036 | MS.gene005326:CDS |
| GCCATATTAGGGCATGACAT+TGG | 0.564799 | 1.1:+62480211 | MS.gene005326:CDS |
| GAAGATGGGATGTACACTCC+TGG | 0.565328 | 1.1:-62485573 | None:intergenic |
| CCGACAAATACACAACCATT+CGG | 0.567790 | 1.1:+62483273 | MS.gene005326:CDS |
| AGTTGGGTTCAGCTTATGGT+AGG | 0.568189 | 1.1:-62484319 | None:intergenic |
| ATGGCTGAAGTTGTTTAGCA+AGG | 0.569030 | 1.1:-62488161 | None:intergenic |
| GGGAAAGCACTTACAACACC+TGG | 0.570813 | 1.1:-62484542 | None:intergenic |
| AACCCCTGCATCTCACCCGC+AGG | 0.573533 | 1.1:+62479099 | MS.gene005326:CDS |
| ATTAGGCCCACCACCAACCA+CGG | 0.575070 | 1.1:-62482058 | None:intergenic |
| CAATCCTTATCCCAATGCCT+TGG | 0.576534 | 1.1:-62487033 | None:intergenic |
| TTGCTAAACAACTTCAGCCA+TGG | 0.577584 | 1.1:+62488163 | MS.gene005326:CDS |
| TCAATCCCGTGGTTGGTGGT+GGG | 0.577700 | 1.1:+62482052 | MS.gene005326:CDS |
| TGAGAGGTGCGCAGCGAATG+GGG | 0.579429 | 1.1:+62481821 | MS.gene005326:CDS |
| GTACCTGCGGGTGAGATGCA+GGG | 0.580749 | 1.1:-62479102 | None:intergenic |
| CAACTTCAGCCATGGAATAG+GGG | 0.581494 | 1.1:+62488171 | MS.gene005326:CDS |
| TACCTGCGGGTGAGATGCAG+GGG | 0.581809 | 1.1:-62479101 | None:intergenic |
| GACATTGGATGTGAGTTACA+TGG | 0.582230 | 1.1:+62482215 | MS.gene005326:CDS |
| TGATAGCCAAACTCTTCTCT+GGG | 0.583126 | 1.1:-62480084 | None:intergenic |
| TCTCATTGGATACGCCTCGT+TGG | 0.585869 | 1.1:-62481126 | None:intergenic |
| GATCCTTCCAGATATGGTAA+GGG | 0.586115 | 1.1:+62481376 | MS.gene005326:intron |
| AAAGATGGGGAGATCACACC+AGG | 0.587344 | 1.1:+62480010 | MS.gene005326:CDS |
| ATCAGATTCAATCCCGTGGT+TGG | 0.587531 | 1.1:+62482045 | MS.gene005326:intron |
| TTAGGCCCACCACCAACCAC+GGG | 0.589559 | 1.1:-62482057 | None:intergenic |
| AGCTTACTGTAACAACGTAA+AGG | 0.590674 | 1.1:+62481472 | MS.gene005326:CDS |
| CTGCCAACAACCTGGTGGTG+TGG | 0.593769 | 1.1:+62480189 | MS.gene005326:CDS |
| TCGTTCTTATTCCAGTCACA+AGG | 0.594752 | 1.1:+62479054 | MS.gene005326:CDS |
| ATTACAGGCTGCCAACAACC+TGG | 0.596169 | 1.1:+62480181 | MS.gene005326:CDS |
| CAGGTTGAAATGCTGCAGAA+AGG | 0.597743 | 1.1:+62484255 | MS.gene005326:intron |
| ACGCGTACCTGGCCACCTTG+TGG | 0.599158 | 1.1:+62482254 | MS.gene005326:CDS |
| CAGGTAATAACTATTGAACC+AGG | 0.600635 | 1.1:+62485555 | MS.gene005326:intron |
| TCCAGGTCACTATCTAGGAA+TGG | 0.601316 | 1.1:+62484475 | MS.gene005326:intron |
| AGAAACTAACAAGCATTGTG+TGG | 0.607256 | 1.1:+62483225 | MS.gene005326:CDS |
| AATGCTGCAGAAAGGACTCA+AGG | 0.627439 | 1.1:+62484263 | MS.gene005326:CDS |
| TTATTCTAGGAACGATCAGA+AGG | 0.629437 | 1.1:+62482095 | MS.gene005326:CDS |
| ATCCTTCCAGATATGGTAAG+GGG | 0.630528 | 1.1:+62481377 | MS.gene005326:CDS |
| CTGTATCAGATTCAATCCCG+TGG | 0.633552 | 1.1:+62482041 | MS.gene005326:intron |
| ATTGTAGCTTCTAAAAGATG+GGG | 0.641791 | 1.1:+62479997 | MS.gene005326:intron |
| ATTTGTCGGATACTAGCACC+AGG | 0.642966 | 1.1:-62483259 | None:intergenic |
| GATCTCTAATGCAGGTACCA+AGG | 0.651356 | 1.1:+62487016 | MS.gene005326:intron |
| AACCCCTTACCATATCTGGA+AGG | 0.655197 | 1.1:-62481379 | None:intergenic |
| ATCAGCATATACGGAACTGG+AGG | 0.655539 | 1.1:+62481436 | MS.gene005326:CDS |
| CACACCAGGCATATCTAGTG+AGG | 0.673963 | 1.1:+62480024 | MS.gene005326:CDS |
| ATCTGTCATCATCTTTACTG+GGG | 0.686632 | 1.1:-62480116 | None:intergenic |
| ACATTGGATGTGAGTTACAT+GGG | 0.693361 | 1.1:+62482216 | MS.gene005326:CDS |
| GAATACGAATGCAAAATGAG+AGG | 0.696219 | 1.1:+62481805 | MS.gene005326:CDS |
| GCAGAAGAGAAGCTACCACA+AGG | 0.709712 | 1.1:-62482269 | None:intergenic |
| GAAGAGAAGCTACCACAAGG+TGG | 0.733869 | 1.1:-62482266 | None:intergenic |
| TCCAATGAGAAAGTTGAACG+AGG | 0.743293 | 1.1:+62481139 | MS.gene005326:CDS |
| ACAGGCTGCCAACAACCTGG+TGG | 0.744058 | 1.1:+62480184 | MS.gene005326:CDS |
| GCTGCAGAAAGGACTCAAGG+AGG | 0.745589 | 1.1:+62484266 | MS.gene005326:CDS |
| GAATACATTCAAGGCCAACG+AGG | 0.787952 | 1.1:+62481112 | MS.gene005326:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 62478877 | 62488231 | 62478877 | ID=MS.gene005326 |
| chr1.1 | mRNA | 62478877 | 62488231 | 62478877 | ID=MS.gene005326.t1;Parent=MS.gene005326 |
| chr1.1 | exon | 62478877 | 62478924 | 62478877 | ID=MS.gene005326.t1.exon1;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62478877 | 62478924 | 62478877 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62479043 | 62479120 | 62479043 | ID=MS.gene005326.t1.exon2;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62479043 | 62479120 | 62479043 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62480004 | 62480267 | 62480004 | ID=MS.gene005326.t1.exon3;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62480004 | 62480267 | 62480004 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62481068 | 62481160 | 62481068 | ID=MS.gene005326.t1.exon4;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62481068 | 62481160 | 62481068 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62481377 | 62481583 | 62481377 | ID=MS.gene005326.t1.exon5;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62481377 | 62481583 | 62481377 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62481739 | 62481842 | 62481739 | ID=MS.gene005326.t1.exon6;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62481739 | 62481842 | 62481739 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62482050 | 62482116 | 62482050 | ID=MS.gene005326.t1.exon7;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62482050 | 62482116 | 62482050 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62482188 | 62482295 | 62482188 | ID=MS.gene005326.t1.exon8;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62482188 | 62482295 | 62482188 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62483202 | 62483294 | 62483202 | ID=MS.gene005326.t1.exon9;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62483202 | 62483294 | 62483202 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62484258 | 62484348 | 62484258 | ID=MS.gene005326.t1.exon10;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62484258 | 62484348 | 62484258 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62484480 | 62484550 | 62484480 | ID=MS.gene005326.t1.exon11;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62484480 | 62484550 | 62484480 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62485558 | 62485613 | 62485558 | ID=MS.gene005326.t1.exon12;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62485558 | 62485613 | 62485558 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62487030 | 62487090 | 62487030 | ID=MS.gene005326.t1.exon13;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62487030 | 62487090 | 62487030 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| chr1.1 | exon | 62488118 | 62488231 | 62488118 | ID=MS.gene005326.t1.exon14;Parent=MS.gene005326.t1 |
| chr1.1 | CDS | 62488118 | 62488231 | 62488118 | ID=cds.MS.gene005326.t1;Parent=MS.gene005326.t1 |
| Gene Sequence |
| Protein sequence |
