AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008303

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008303.t1 MTR_3g065130 98.381 741 10 1 1 741 1 739 0.0 1501
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008303.t1 AT5G37510 78.523 745 156 1 1 741 1 745 0.0 1208
MS.gene008303.t1 AT5G37510 78.523 745 156 1 1 741 1 745 0.0 1208

Find 216 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCCTTCTCGTGCTTTCT+TGG 0.148903 3.1:-54578161 None:intergenic
TGCAAAGTTTGTATATTTAA+TGG 0.190393 3.1:+54583621 MS.gene008303:CDS
TTTGAAATAGTCTTGGAAAA+TGG 0.193927 3.1:-54583391 None:intergenic
TCTCCACCCTGATCACAAAT+TGG 0.219635 3.1:-54578220 None:intergenic
GAAGAAATTGTTGGAATATC+TGG 0.232864 3.1:+54582710 MS.gene008303:CDS
TGCGCCATGCCCGCTCTTCC+TGG 0.245156 3.1:+54576493 MS.gene008303:CDS
TTGGGGCTGAGCGGCGGGTT+CGG 0.245373 3.1:-54576209 None:intergenic
GAGGAGGAGGCGGGTGGTGG+TGG 0.250324 3.1:-54576149 None:intergenic
TGGGGCTGAGCGGCGGGTTC+GGG 0.281624 3.1:-54576208 None:intergenic
TATTCTTCAACTCCCAATTC+CGG 0.285395 3.1:-54580110 None:intergenic
GAGGAGGCGGGTGGTGGTGG+TGG 0.291433 3.1:-54576146 None:intergenic
TTGCATTTAAAGCCCGGAAT+TGG 0.299665 3.1:+54580097 MS.gene008303:CDS
TCTGCTATCAATTCGAATAT+TGG 0.314496 3.1:-54580166 None:intergenic
GGGTTCGGGTTTGCGGAGTT+CGG 0.321764 3.1:-54576194 None:intergenic
GACGAGAGGTTGTTCAGGTT+GGG 0.322345 3.1:-54576227 None:intergenic
GCAAAGTTTGTATATTTAAT+GGG 0.327097 3.1:+54583622 MS.gene008303:CDS
TCTCGTGCTTTCTTGGCTAT+AGG 0.335594 3.1:-54578154 None:intergenic
GATATTCCAACAATTTCTTC+TGG 0.338173 3.1:-54582707 None:intergenic
GGGTGGAGAGTGTGATCTTC+AGG 0.340342 3.1:+54578234 MS.gene008303:CDS
TTGAAATAGTCTTGGAAAAT+GGG 0.343715 3.1:-54583390 None:intergenic
ATACAAACTTTGCAGATTCA+AGG 0.344116 3.1:-54583612 None:intergenic
CGTTTACCCTATGATACAAT+TGG 0.350227 3.1:+54583889 MS.gene008303:CDS
CCATCAGGACCACGAATCAT+AGG 0.350258 3.1:-54582617 None:intergenic
CATAGCAGACTTTCCATTGC+CGG 0.350464 3.1:+54576415 MS.gene008303:CDS
GGTTCGGGTTTGCGGAGTTC+GGG 0.354183 3.1:-54576193 None:intergenic
GTTCAGGATCTTGGCATGTT+AGG 0.371949 3.1:+54579964 MS.gene008303:CDS
CTGGCGTGAAGCCCTTTCTT+TGG 0.376057 3.1:+54582658 MS.gene008303:CDS
ATTCTTCAACTCCCAATTCC+GGG 0.378095 3.1:-54580109 None:intergenic
CACTAAAAGATTTCTTAAAC+AGG 0.378664 3.1:+54582762 MS.gene008303:CDS
CCAGCACCAACAATGATAAC+AGG 0.379076 3.1:-54583424 None:intergenic
GTTGCAGGGGTTCAGGATCT+TGG 0.379251 3.1:+54579955 MS.gene008303:CDS
TCGATAGCGTCATCGGGATT+GGG 0.379722 3.1:-54576295 None:intergenic
GAGGAGGAGGAGGCGGGTGG+TGG 0.381251 3.1:-54576152 None:intergenic
GGAACGATGCGCATGACTTC+AGG 0.383852 3.1:-54580192 None:intergenic
TCACAAATTGGGCAATCTAA+TGG 0.385374 3.1:-54578208 None:intergenic
AAGACACATACGACAATTTC+CGG 0.386902 3.1:-54576434 None:intergenic
TCAGGTTGGGGCTGAGCGGC+GGG 0.387179 3.1:-54576214 None:intergenic
CTCGATAGCGTCATCGGGAT+TGG 0.388965 3.1:-54576296 None:intergenic
GGGTTTGCGGAGTTCGGGTT+TGG 0.389818 3.1:-54576188 None:intergenic
CAGGCTGCTGCACTTGACCT+TGG 0.391023 3.1:+54583565 MS.gene008303:CDS
AGATTGCCCAATTTGTGATC+AGG 0.398118 3.1:+54578213 MS.gene008303:CDS
CAGGATCAGTCAATGGCATT+TGG 0.398437 3.1:+54578253 MS.gene008303:CDS
ATTATGAATACTAGCATTGC+TGG 0.399661 3.1:+54582854 MS.gene008303:CDS
CTTCAAGCGTGTGAGGTTGC+TGG 0.401893 3.1:+54576367 MS.gene008303:CDS
CATGGAGAAGTAATACATTA+AGG 0.402841 3.1:-54583537 None:intergenic
TACCAGGGCATCAAAGATAA+TGG 0.403072 3.1:+54584074 MS.gene008303:CDS
ATCAGAGGTTGCAGGGGTTC+AGG 0.403687 3.1:+54579948 MS.gene008303:CDS
CTGCTATGATAACAAAATCT+AGG 0.407506 3.1:-54576400 None:intergenic
AAAAGATTTCTTAAACAGGC+TGG 0.409928 3.1:+54582766 MS.gene008303:CDS
TCGTCCATTTGCGCAAGGTT+TGG 0.410243 3.1:-54583943 None:intergenic
TGCATTTAAAGCCCGGAATT+GGG 0.411080 3.1:+54580098 MS.gene008303:CDS
GTTTACCCTATGATACAATT+GGG 0.415159 3.1:+54583890 MS.gene008303:CDS
GTTAAACCAGAAGAAATTGT+TGG 0.417305 3.1:+54582701 MS.gene008303:CDS
TCCACCCTAACAATGGGGTT+TGG 0.419261 3.1:+54576094 None:intergenic
AATGTATGGGGCGAAGGAAT+TGG 0.421514 3.1:+54582797 MS.gene008303:CDS
GAGCGGCGGGTTCGGGTTTG+CGG 0.422300 3.1:-54576201 None:intergenic
AGCGCCAAACCTTGCGCAAA+TGG 0.424180 3.1:+54583939 MS.gene008303:CDS
TTCAGGTTGGGGCTGAGCGG+CGG 0.426747 3.1:-54576215 None:intergenic
AGGTGGATCCATCACCATTC+GGG 0.428089 3.1:+54584016 MS.gene008303:CDS
GGCTGGGGTCAAATAATGTA+TGG 0.432511 3.1:+54582783 MS.gene008303:CDS
AGCGCCAGCGACGGGTGTAC+GGG 0.434794 3.1:-54576260 None:intergenic
GCGCAAGAAGCAACAGGCTT+AGG 0.435847 3.1:-54576475 None:intergenic
AATTATCTTCCAATCATCTC+TGG 0.439607 3.1:-54583839 None:intergenic
AGATCGACGAGAGGTTGTTC+AGG 0.439627 3.1:-54576232 None:intergenic
AAACAACAAAAGCATCCTTC+GGG 0.441280 3.1:-54583672 None:intergenic
AGCCATTATCTTTGATGCCC+TGG 0.443739 3.1:-54584076 None:intergenic
AAAGATTTCTTAAACAGGCT+GGG 0.445416 3.1:+54582767 MS.gene008303:CDS
TTCTCACTGAATGCAGCTGC+TGG 0.446093 3.1:-54583745 None:intergenic
CGACGAGAGGTTGTTCAGGT+TGG 0.449270 3.1:-54576228 None:intergenic
ACTAATGCTGATTTCAGATC+AGG 0.449684 3.1:+54582827 MS.gene008303:CDS
CAAGGTCAAGTGCAGCAGCC+TGG 0.450413 3.1:-54583564 None:intergenic
CTCTCTCGTCCATTTGCGCA+AGG 0.451054 3.1:-54583948 None:intergenic
TTTGGCATTTGAAATAGTCT+TGG 0.453444 3.1:-54583398 None:intergenic
TGAAGAACAGAGAATCCTTT+CGG 0.453943 3.1:-54576349 None:intergenic
AACTATGATAACGAACATCT+TGG 0.457876 3.1:+54583325 MS.gene008303:CDS
TTGAACGGAGGAGGAGGAGG+CGG 0.458930 3.1:-54576159 None:intergenic
TAAACAACAAAAGCATCCTT+CGG 0.459831 3.1:-54583673 None:intergenic
TTCCGGGCTTTAAATGCAAA+AGG 0.460040 3.1:-54580093 None:intergenic
GCAATTTCAAGAAGTGTTTG+AGG 0.460236 3.1:-54583355 None:intergenic
TCTCCAACTGTTGGAACTGC+AGG 0.461407 3.1:-54583814 None:intergenic
CTCCACCCTGATCACAAATT+GGG 0.462587 3.1:-54578219 None:intergenic
GGCATGGCGCAAGAAGCAAC+AGG 0.463187 3.1:-54576481 None:intergenic
TGGTGGTGATTGAACGGAGG+AGG 0.463432 3.1:-54576168 None:intergenic
TAGCGCCAGCGACGGGTGTA+CGG 0.465790 3.1:-54576261 None:intergenic
CAAGCCAAGGTCGGTTACAT+TGG 0.466458 3.1:+54583286 MS.gene008303:CDS
CCTGTTATCATTGTTGGTGC+TGG 0.468259 3.1:+54583424 MS.gene008303:CDS
CGAGCTCTGTCCGAGATAGC+AGG 0.468342 3.1:+54583862 MS.gene008303:CDS
CTGTTATCATTGTTGGTGCT+GGG 0.468439 3.1:+54583425 MS.gene008303:CDS
GAAAACCTCGATAGCGTCAT+CGG 0.473854 3.1:-54576302 None:intergenic
TGGTGATTGAACGGAGGAGG+AGG 0.476683 3.1:-54576165 None:intergenic
CAACCTCTGCTACCAAAGAA+AGG 0.479683 3.1:-54582670 None:intergenic
AAGGTGGATCCATCACCATT+CGG 0.479718 3.1:+54584015 MS.gene008303:CDS
CTCTGTTCTTCAAGCGTGTG+AGG 0.480791 3.1:+54576360 MS.gene008303:CDS
TAAACGCACCCCTGCTATCT+CGG 0.480951 3.1:-54583872 None:intergenic
AAGGTCAAGTGCAGCAGCCT+GGG 0.483480 3.1:-54583563 None:intergenic
AAGCCAAGGTCGGTTACATT+GGG 0.484241 3.1:+54583287 MS.gene008303:CDS
TGAACGGAGGAGGAGGAGGC+GGG 0.484386 3.1:-54576158 None:intergenic
TCCACCTTCTTAGTGAAGGT+TGG 0.484390 3.1:-54584000 None:intergenic
CAATGTAACCGACCTTGGCT+TGG 0.485781 3.1:-54583285 None:intergenic
ATACCTGGGTGCCAACCAAC+AGG 0.486083 3.1:-54582898 None:intergenic
CGGGTTTGGTGGTGATTGAA+CGG 0.490273 3.1:-54576174 None:intergenic
ACGAGAGGTTGTTCAGGTTG+GGG 0.490622 3.1:-54576226 None:intergenic
TTGGAGACTCCAGAGATGAT+TGG 0.491323 3.1:+54583830 MS.gene008303:CDS
TTTGAAGTGAGTGCTCCTAC+AGG 0.494027 3.1:-54580069 None:intergenic
GATTGCCCAATTTGTGATCA+GGG 0.494519 3.1:+54578214 MS.gene008303:CDS
ATATGACTGACGCAATTACC+AGG 0.496323 3.1:+54584058 MS.gene008303:CDS
ATGATGGTTTGAAGAGGCAG+AGG 0.496611 3.1:+54582585 MS.gene008303:CDS
CATCGTTCCTCGTTTAAATG+AGG 0.497665 3.1:+54580206 MS.gene008303:CDS
TTTCCTGTTGGTTGGCACCC+AGG 0.498602 3.1:+54582895 MS.gene008303:CDS
TGATTGAACGGAGGAGGAGG+AGG 0.499246 3.1:-54576162 None:intergenic
TTTGCGGAGTTCGGGTTTGG+TGG 0.501325 3.1:-54576185 None:intergenic
TCAAATAATGTATGGGGCGA+AGG 0.502062 3.1:+54582791 MS.gene008303:CDS
CAAACCATCATAACAAAAGC+GGG 0.502070 3.1:-54582573 None:intergenic
AGTGAAGATGGCAATGCAGC+TGG 0.503709 3.1:-54583973 None:intergenic
AGAATCTAACCAGGAAGAGC+GGG 0.505711 3.1:-54576502 None:intergenic
CGTGGTAGTGGAGAAGAGAT+TGG 0.506494 3.1:+54579988 MS.gene008303:CDS
GAAAGCACGAGAAGGAGTGA+TGG 0.508097 3.1:+54578165 MS.gene008303:CDS
GCTGCTTTAAGCCTGTGAAC+TGG 0.508870 3.1:+54582639 MS.gene008303:CDS
ACGGAGGAGGAGGAGGCGGG+TGG 0.509605 3.1:-54576155 None:intergenic
ACAGGCTTAAAGCAGCCATC+AGG 0.509743 3.1:-54582632 None:intergenic
GCAGATGCTTTCCTGTTGGT+TGG 0.510700 3.1:+54582887 MS.gene008303:CDS
GGTGGTGGTGGTTTGACGAA+AGG 0.512548 3.1:-54576134 None:intergenic
AGGGCTTCACGCCAGTTCAC+AGG 0.513098 3.1:-54582650 None:intergenic
CCTATGATTCGTGGTCCTGA+TGG 0.514497 3.1:+54582617 MS.gene008303:CDS
AGCCCTTTCTTTGGTAGCAG+AGG 0.515340 3.1:+54582667 MS.gene008303:CDS
TCCAAATTTGCAGCCACGGG+TGG 0.516967 3.1:+54583216 MS.gene008303:intron
ACCGCAATCCCGAATGGTGA+TGG 0.517659 3.1:-54584024 None:intergenic
GTAATAGATATCTGCCCTGT+AGG 0.517990 3.1:+54580054 MS.gene008303:CDS
AAGATTTCTTAAACAGGCTG+GGG 0.519660 3.1:+54582768 MS.gene008303:CDS
GCCACGGGTGGAAGCTGCTA+TGG 0.519839 3.1:+54583228 MS.gene008303:intron
CACGGTGCATTCAATGTACA+AGG 0.523747 3.1:+54578356 MS.gene008303:CDS
AAAACCTCGATAGCGTCATC+GGG 0.526600 3.1:-54576301 None:intergenic
AAGGTTGGTCTCAGTGAAGA+TGG 0.527216 3.1:-54583985 None:intergenic
GTGCAGCAGCCTGGGCAGCA+TGG 0.527867 3.1:-54583555 None:intergenic
TCCATCACCATTCGGGATTG+CGG 0.530979 3.1:+54584023 MS.gene008303:CDS
GATTCGCATCAGAGGTTGCA+GGG 0.532762 3.1:+54579941 MS.gene008303:CDS
TCAAACCATCATAACAAAAG+CGG 0.533289 3.1:-54582574 None:intergenic
ACTCCCCGTACACCCGTCGC+TGG 0.533775 3.1:+54576256 MS.gene008303:CDS
TCTCTGGAGTCTCCAACTGT+TGG 0.533784 3.1:-54583823 None:intergenic
CAGATTGGGGCACAAGTCCA+AGG 0.534798 3.1:-54583582 None:intergenic
GGCATGTTAGGTCGTGGTAG+TGG 0.535963 3.1:+54579976 MS.gene008303:CDS
AACCTCTGCTACCAAAGAAA+GGG 0.540408 3.1:-54582669 None:intergenic
TTGTCGTATGTGTCTTGTTG+AGG 0.541076 3.1:+54576441 MS.gene008303:CDS
GATAACGAACATCTTGGTAC+TGG 0.541143 3.1:+54583331 MS.gene008303:CDS
AAGAATCTAACCAGGAAGAG+CGG 0.541343 3.1:-54576503 None:intergenic
AGGCCCAATGTAACCGACCT+TGG 0.541841 3.1:-54583290 None:intergenic
TGGCCTGCAGTTCCAACAGT+TGG 0.541849 3.1:+54583811 MS.gene008303:CDS
TTGGCGCTACAGTCCTCAGT+CGG 0.542821 3.1:-54583924 None:intergenic
AGCAGCTGCATTCAGTGAGA+AGG 0.545961 3.1:+54583747 MS.gene008303:CDS
TGTACTCTAGCGCCAGCGAC+GGG 0.547564 3.1:-54576268 None:intergenic
ATTACTTCTCCATGCTGCCC+AGG 0.551990 3.1:+54583546 MS.gene008303:CDS
TGGCGCTACAGTCCTCAGTC+GGG 0.554048 3.1:-54583923 None:intergenic
GCTGGGGTCAAATAATGTAT+GGG 0.555168 3.1:+54582784 MS.gene008303:CDS
AACACTTCTTGAAATTGCTG+AGG 0.556487 3.1:+54583360 MS.gene008303:CDS
CATACGACAATTTCCGGCAA+TGG 0.556528 3.1:-54576428 None:intergenic
CTAACCAGGAAGAGCGGGCA+TGG 0.557243 3.1:-54576497 None:intergenic
TCATAGTTGAAATCAGTAGC+AGG 0.557491 3.1:-54583310 None:intergenic
CGATAGCGTCATCGGGATTG+GGG 0.558144 3.1:-54576294 None:intergenic
AGATTCGCATCAGAGGTTGC+AGG 0.558678 3.1:+54579940 MS.gene008303:CDS
GGGAGCTGATGATACAAACT+TGG 0.560243 3.1:+54583642 MS.gene008303:CDS
GGGGAGTTCGCGCCCGACTG+AGG 0.561987 3.1:+54583911 MS.gene008303:CDS
GAGCTCTGTCCGAGATAGCA+GGG 0.566857 3.1:+54583863 MS.gene008303:CDS
ATTGATGTTACTGATGCTGT+TGG 0.567155 3.1:+54580141 MS.gene008303:CDS
TGATCTTCAGGATCAGTCAA+TGG 0.567607 3.1:+54578246 MS.gene008303:CDS
ATGTACTCTAGCGCCAGCGA+CGG 0.568207 3.1:-54576269 None:intergenic
CATCAGGACCACGAATCATA+GGG 0.571545 3.1:-54582616 None:intergenic
AAGGATGCACACAGCAAACA+TGG 0.573067 3.1:+54583791 MS.gene008303:CDS
CTCATGACAAGTGAACTCTC+TGG 0.573975 3.1:+54580027 MS.gene008303:CDS
TTAAATGACCCTATGATTCG+TGG 0.576146 3.1:+54582608 MS.gene008303:CDS
GATCTTGGCATGTTAGGTCG+TGG 0.583375 3.1:+54579970 MS.gene008303:CDS
AACAGCATCAGTAACATCAA+TGG 0.585328 3.1:-54580139 None:intergenic
GTTGAAACCATTGCAAAACA+AGG 0.585854 3.1:+54583487 MS.gene008303:CDS
ACACTTCTTGAAATTGCTGA+GGG 0.586259 3.1:+54583361 MS.gene008303:CDS
GGAATGTCGTCAGATCTGAT+TGG 0.587771 3.1:+54583509 MS.gene008303:CDS
TTCTCAACCGCAATCCCGAA+TGG 0.588558 3.1:-54584030 None:intergenic
TGCAGGACATAAACGAAGAG+TGG 0.593125 3.1:+54582537 MS.gene008303:intron
GAGACCAACCTTCACTAAGA+AGG 0.595141 3.1:+54583996 MS.gene008303:CDS
TTTACCCTATGATACAATTG+GGG 0.596584 3.1:+54583891 MS.gene008303:CDS
TGGATCCACCTTCTTAGTGA+AGG 0.596835 3.1:-54584004 None:intergenic
TTGAAGTGAGTGCTCCTACA+GGG 0.600626 3.1:-54580068 None:intergenic
ATGGCATTTGGCTCTGATCG+TGG 0.602789 3.1:+54578265 MS.gene008303:CDS
TTGTTCAGGTTGGGGCTGAG+CGG 0.604200 3.1:-54576218 None:intergenic
GTCGTCAGATCTGATTGGAA+TGG 0.604807 3.1:+54583514 MS.gene008303:CDS
AATATTCGAATTGATAGCAG+AGG 0.605002 3.1:+54580168 MS.gene008303:CDS
ACCATAGCAGCTTCCACCCG+TGG 0.605776 3.1:-54583229 None:intergenic
GTGTGTTAGATTCGCATCAG+AGG 0.608633 3.1:+54579933 MS.gene008303:CDS
GAAAGCAGATGCTTTCCTGT+TGG 0.608999 3.1:+54582883 MS.gene008303:CDS
TACCCCGTTAAAATTCCGAA+AGG 0.610431 3.1:+54576334 MS.gene008303:CDS
GTCATCACAGTCTTTACCAA+AGG 0.611871 3.1:-54578334 None:intergenic
ACCAACCTTCACTAAGAAGG+TGG 0.614871 3.1:+54583999 MS.gene008303:CDS
GCGCCAGCGACGGGTGTACG+GGG 0.624880 3.1:-54576259 None:intergenic
GGTACATATGAAAATACTGA+AGG 0.625208 3.1:+54583772 MS.gene008303:CDS
GTTGTTTATCAAGGTCACCA+TGG 0.628883 3.1:+54583688 MS.gene008303:CDS
TTTGTTATGATGGTTTGAAG+AGG 0.631528 3.1:+54582579 MS.gene008303:CDS
CAATCCCGATGACGCTATCG+AGG 0.640482 3.1:+54576297 MS.gene008303:CDS
ATAGCCAAGAAAGCACGAGA+AGG 0.643724 3.1:+54578157 MS.gene008303:CDS
CAGCACCAACAATGATAACA+GGG 0.645958 3.1:-54583423 None:intergenic
GCTGCATTCAGTGAGAAGGA+AGG 0.652544 3.1:+54583751 MS.gene008303:CDS
CTGGGGTCAAATAATGTATG+GGG 0.653742 3.1:+54582785 MS.gene008303:CDS
TAGAATACGCAAGACTGTCA+CGG 0.655290 3.1:+54583258 MS.gene008303:CDS
TTGAAACCATTGCAAAACAA+GGG 0.663961 3.1:+54583488 MS.gene008303:CDS
ATTCGCATCAGAGGTTGCAG+GGG 0.664579 3.1:+54579942 MS.gene008303:CDS
TGATATGAAGAGATCTGTGG+TGG 0.665318 3.1:+54578297 MS.gene008303:CDS
AGCTCTGTCCGAGATAGCAG+GGG 0.680552 3.1:+54583864 MS.gene008303:CDS
GATAAACACTCTTATCACCA+TGG 0.680783 3.1:-54583705 None:intergenic
ACGGCAAACCAAGCCAAGGT+CGG 0.681995 3.1:+54583277 MS.gene008303:CDS
TGTCACGGCAAACCAAGCCA+AGG 0.683373 3.1:+54583273 MS.gene008303:CDS
TTACCCTATGATACAATTGG+GGG 0.685077 3.1:+54583892 MS.gene008303:CDS
TATGACTGACGCAATTACCA+GGG 0.688145 3.1:+54584059 MS.gene008303:CDS
TGCCCAATTTGTGATCAGGG+TGG 0.691459 3.1:+54578217 MS.gene008303:CDS
GTTTGGTGGTGATTGAACGG+AGG 0.700949 3.1:-54576171 None:intergenic
CACTGATATGAAGAGATCTG+TGG 0.705213 3.1:+54578294 MS.gene008303:CDS
CGGGGAGTGAGATCGACGAG+AGG 0.710019 3.1:-54576241 None:intergenic
AAACTTGGACAAGATCCCGA+AGG 0.712042 3.1:+54583657 MS.gene008303:CDS
TGGTAAAGACTGTGATGACA+CGG 0.740395 3.1:+54578338 MS.gene008303:CDS
AATCTAACCTCATTTAAACG+AGG 0.753181 3.1:-54580213 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.1 gene 54576106 54584128 54576106 ID=MS.gene008303
chr3.1 mRNA 54576106 54584128 54576106 ID=MS.gene008303.t1;Parent=MS.gene008303
chr3.1 exon 54576106 54576514 54576106 ID=MS.gene008303.t1.exon1;Parent=MS.gene008303.t1
chr3.1 CDS 54576106 54576514 54576106 ID=cds.MS.gene008303.t1;Parent=MS.gene008303.t1
chr3.1 exon 54578131 54578377 54578131 ID=MS.gene008303.t1.exon2;Parent=MS.gene008303.t1
chr3.1 CDS 54578131 54578377 54578131 ID=cds.MS.gene008303.t1;Parent=MS.gene008303.t1
chr3.1 exon 54579933 54580227 54579933 ID=MS.gene008303.t1.exon3;Parent=MS.gene008303.t1
chr3.1 CDS 54579933 54580227 54579933 ID=cds.MS.gene008303.t1;Parent=MS.gene008303.t1
chr3.1 exon 54582542 54582916 54582542 ID=MS.gene008303.t1.exon4;Parent=MS.gene008303.t1
chr3.1 CDS 54582542 54582916 54582542 ID=cds.MS.gene008303.t1;Parent=MS.gene008303.t1
chr3.1 exon 54583229 54584128 54583229 ID=MS.gene008303.t1.exon5;Parent=MS.gene008303.t1
chr3.1 CDS 54583229 54584128 54583229 ID=cds.MS.gene008303.t1;Parent=MS.gene008303.t1
Gene Sequence

>MS.gene008303

ATGGGGTTTGGTTTATTAGCTTCAAAGACCTTTCGTCAAACCACCACCACCACCCGCCTCCTCCTCCTCCGTTCAATCACCACCAAACCCGAACTCCGCAAACCCGAACCCGCCGCTCAGCCCCAACCTGAACAACCTCTCGTCGATCTCACTCCCCGTACACCCGTCGCTGGCGCTAGAGTACATTTCCCCAATCCCGATGACGCTATCGAGGTTTTCGTCGATGGATACCCCGTTAAAATTCCGAAAGGATTCTCTGTTCTTCAAGCGTGTGAGGTTGCTGGTGTTGATATTCCTAGATTTTGTTATCATAGCAGACTTTCCATTGCCGGAAATTGTCGTATGTGTCTTGTTGAGGTTGAGAAATCTCCTAAGCCTGTTGCTTCTTGCGCCATGCCCGCTCTTCCTGGTTAGATTCTTACTTTTTTTTTCTGTTGCTGGATTTGCATTTCATTGATGATTTTTGTGTCTGTTTTGTTTATCTAAGAAATTTATTTTATTTATTTGAGTTTTGTTGTGTGTAAATGGAGTGTTGGATGGTAAAGAACAACACCGGATTAAAATAAGTGTAGTAGAGATGAAGATATTGCATTGGATGTGTGGTAATGCTAGACCATGATTTAGGGTGCGTTTGTTTTTGAATTTCAACGAAAAAAATCACTTTTGTCATTTGTAAGTGTTAGGTTAGGATTTAAAAAACAATCATTTTGAGTCAAATGAAATGCTATTAAGATTAACCTTTCCCAAAATCTATTTTTTCAAACACAAAGAGGCAAAACATGAATTAGTTAAAAAATCATTTTTTATGGGCGGTAAACAAACATAAATAGCTTCAGATTTTTTTAACACAAAATCTATTTTTTTAAAGCTGAAACAAACAGACTCTTAATTAGAAATGACAATGTTAGATAAAGAGTTGGGGTAGCCACCTATTGGAGAAAAGATGGTAGAAACTCGGCATATGTGGTTTGGGCATGTAGAGAGAAGACTTGTAGATTCAGCAATAAGAAGAGTATATCAGATGGAGGATAGTCAGATCACTAGAGGTAGAGGAAGACCTAGAAAAACTATGAGAGAAACTATTAAGAACGATCTAGAGATTAATGAGTTGGATAAGGATATAATATATGATAGAACATTATGCCGGTGTTTGATACATGTAGCCAACCACACTTGGTGGAATAAGGCTTGCTTGTTGTTTGAGTGATTCTTTTGATTAGTTCAAGGCCAAAAGTATTAAACTTAATAGTTGATGATTGCATTGTTGCTTGGCGTTATTTTCAAATTTCTAGTTGGTTGAACATGTTTGACTTTTGTTCAGTTTCTGTTATTTGCAAATTGTAGTTTGTAGATAACGGTGATACTAGTGAACTTCCATCTAGTTACTCCCTCCGTCTCACAATAATTGTCTCTTTACCCATTTTTCTTTTTCTCAAAATAATTGTCACTTTAGAATACCAATGCATCATTTATTATTTTTTTCCATTATTATACCCTTATTTATTGAATTTCATCCAACTTAACTACTCCACTAACTACAATTAACAAGGGTGGTTTAGTAAATGATACTAATTTTACCATCAAAATCAACACAATTAATTATTTCCTTAATAACCGTGCATAAACCTTAAACGACTATTATTTAGAGACGGAGGGAGTATGTGTCTAATAGTCTTACTTTTCCTTGTAACCATCTAGTTGACATTTCTTTTTAGTTATAATTTAGCTTTTGTCTGTCATCAAGTATGCTGTCTTTTTTTTTTGTTCAAAAATACATTCTAGTTTTGCATTACATCTTCTACTTCTTTCTACTTTTATTTTTTAATTAGAGAGATATTTGAGATAAAATTATCATATGTTTAGTTGGTTTGGGGATAGCATCTATATTTTTTGATCTGATCTTACTTTTAATTTCATTTGGTATTGAGACACTGACACATACATATAAACAACCTTGATTTTGTTTAGTTGAAGTTTTCAACCATTTATTGTTAAAAATGTAATATAATCATATGTTCAATTTAGGGATGAAAATCAAGACTGACACACCTATAGCCAAGAAAGCACGAGAAGGAGTGATGGAGTTTTTATTGATGAATCATCCATTAGATTGCCCAATTTGTGATCAGGGTGGAGAGTGTGATCTTCAGGATCAGTCAATGGCATTTGGCTCTGATCGTGGTCGTTTCACTGATATGAAGAGATCTGTGGTGGATAAAAATCTTGGACCTTTGGTAAAGACTGTGATGACACGGTGCATTCAATGTACAAGGTTCATTTTTCCCTGCAAACTTGATTATCTTTACGTACACATTTTGATAACTATATCATACCTTGGAAGCTAGAAATTTGTTGTGACTTTTATCTTCTAAAAGCAAGTATCAAGTGCTCTGCTAATTCATGTTAATAGTATGTATGATATTCTTGTAAGAATTAAGGACCTCGTTGAGTTGCATATATATATATATGAGTCAGGATCCGTTGACACCAGGTGTCAACGGATTCGTTGACACCAAATCTTAACCGTTTATTTTATTTAATCAAAGGCTAATTATAATGCCACATTTTTATTTATTGTGTATCCTTTTTCTCAATCACACTCTAATAAAGACCATCTCAATTATACAAACAGAATCACCTCTATTAATTTCCAACAAACGCAAATCTGGATTTTTCTCTACGAGAAGCAATTCAATTTAGGTTAAAGGGTTCAATTTTAACAAAACGCATAAATTTTACCTCTATTGGATCGTTTCCAATTTTCTAGAAAACATGATGTTGTGGAAGCATAAATTCATAAATCAATAAAAATATGCCGATTTTGCTTTTCTAAATTCTAATTGTTTACTCTTGTCTTTTACGATTTATTCTAGCCCAGATGTGTCTGCCCCTGTTTCTCTTTATAGTCTATTTGTATCAACACATAAATTTGTGATTAAAATTCCCTAACGTGTATTCAACTACTGAGTCAATTATTCCATTGAAATTCAAAACATGGTGGTATCGTTATAATCTATCCCCTCTTATTAATTCCACGTTTTGATTGCAATTACTGACAAAGGTATTGAAAAACTATGGGCTGCAAAATTGAGAGTGTTGAGAAGAACATCTACATGACTATAATTAATTTTACAAACATCAGTTCTTAGCATCCATTTTGAAATCGAGAAAAAACCTCAATTCTCATGATGATTTGTTTGTGTTTGAAATCAATCTTTTTCAGCAATGAATATTATAATTAAAGAGCTTGGTTGACTTACCATAAAAGAAATAAAGAGCCAAATTCACATGTACATCAAGAATTAGATATTTCATGAGGTCTTAAATGAGTAATTATGAGCCTTTGATTAAAAATAGATGGATGGTTAAGATTTGGTGTCAACGAATTCGTTGACACCTGGTGTCAACGGATCCTACCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTAAATTAAGTGGTGGTCATTGTTACATTATGATAGTTTCTGAGGGAACTTACGGGTCAACTTTTTTTGCATATGATTAAAATAATATTCTGGTGTGATAATTTAGTTTGGTTGTATGTTTTACTTGCTCTCATGTCAGCTCAGGGCCAGCTAGGTTTCTTTTAATTTATGGTTAACGACATCTGGTAGCAATAGGTGTTGGCATTTTTGTAAAACCCAACCTTCAACATTTGGGAGCCATCCTCTTTTTGTTGTCAATTAAATGCTATATGTATTGTGATTATTTTATCTGAGTTCCTTAATGGATCCGATGAAATATTACTTTCTTCCCACAGGTGTGTTAGATTCGCATCAGAGGTTGCAGGGGTTCAGGATCTTGGCATGTTAGGTCGTGGTAGTGGAGAAGAGATTGGAACATATGTTGAAAAACTCATGACAAGTGAACTCTCTGGAAATGTAATAGATATCTGCCCTGTAGGAGCACTCACTTCAAAACCTTTTGCATTTAAAGCCCGGAATTGGGAGTTGAAGAATACAGAGACCATTGATGTTACTGATGCTGTTGGTTCCAATATTCGAATTGATAGCAGAGGACCTGAAGTCATGCGCATCGTTCCTCGTTTAAATGAGGTTAGATTCTGATGGAAATGTTTTATTAAACTTCTTGCGGTTGCCAAAGTTTCAATTTTCTTGTGGTTCTTAACATTATTTAACTCTTGCAAGAAAAACCATGGTACCTTAAGTACTTATGAGTGTTGTCCTATGATTTCGTAGTATGAGTTAATTTGAAAAAATTGCTATTTATTAAGTCAATCTACACTACAACTTATAGGAATCGATGCTTACCAAAAGTTGGGACCAGTGAATTCTGACCTTGATTTATTTAATTGGTTGTAGGATTTTTTTCTTCTTTGCGTTAGAACATAACATACTATTATGACATAAGCGAGTTGATTTTCAAATTTTATTAATTGGCAGCCTCTGAAACACATTACACATGTTGTGTTTGGAAAACTGATGTTGATTTGTTTTCTTCCCCTGATATTAACCTGATTACTTTATATCACTAGCAATGATTGCATTGATGTGGGTTTTGAAAATAAGTAGAAAAAGACCTCCTATTATCACAATTTATTATGGGACTGAAGGAATTGTCTGAGTAGTTATTCAGTTCTTTCTAACTGAATATTTATACTTCCAATTGTCTGAGTAGTTATTCAGTTAATTTCTAACTGAATAATTATACTTCCAGAAGAATATGTGAGCATTTTACTTGCGATCTCAAGCCTTTGGTTATGGGCAATTGCGTTAAGGAGACCTTTAGCTTACCAGTCTGTAAATGCTATGGGGATGAGTATTTGAGGGATAACAATTAAGAAGCTGGTTTTTTAAGTGTTATTTACTGGGCCATTGATTGATCATGAATCATGAAGTAACCTTGGACTGTATAAAATGTGCAGATGTGAATGTGTTTTTGCAGAAGTTGTGATCCTCCCATGCTAATTTTTCACAAGATAGGGTCGCAGTGAACAGGGACGGATACACATTCAACTAAATGGGGGCAGCTGCCCCCAAACTTTTTAAAAATGTTATTTTTAGTCTTTCAATAGCGTGCTATAGCGGAATAGCGTGGCGAAGGAGGCTTCCCCTGCGAAGCCATTTTCCTGCAATGGTGCTTCAACAATGGCTATGATAGTGGCCGCAAATAGCGGTGGAAACATGACTCAATCGCGGGTTCACGTCCGCTTCTAGAGCTCCTGCCGTTTTCCGGCTACCACCCTCATGTTGTGAAGAAACAAGTAATTAAAAGAAATGAATGATGATGACGAAAATATGCAGGGGCACGGTGACTGATGTAGTGTTGCAGAGGTGCCGTTTGGAGATGATGTTTTATATTTAGGGTTTTGAGAATGTACATCTTAGCTTTAGGGTTTGTTTCACATAATGAGTCTATTATAAACCAAACAATGGGTCAAGTATCTTCCTAATGTCTAACAAATGGGCCAAGTATGTTCCTAATTCCTAATGTCTAACAAATGTTTACGGCGGTTAGAGTTAGAAATTCATATGGGAAATGACAAAGTTGATTATAGTTATTAAGTATGATTTTTTTTTTTTTGAGAGAAGTTATTAAGTATGATGGTCTCTTTAGTTTTGTGTAATTTCTTAGTTTTGAGTATTTATGTTTCCTTTGTACACTGATTTATAGTCTTCGCTATAATAGCTATCTCGCTATAGCATTTTAAGGGGTCGACGCTACACGACGCTATCTAGTTTTAACAACATGGGTTTCCCCTTGTTACCGCCAAAATATCGAATAATTTTATTTGTGAAGAATCAAATGCACAACAATATGAGAGAGAGATTATTGAATGCTTGTTTGGTCACAAATATATAAATATATTTTTGATTGTGTAGAAGAAAAAAAATATCAAACAATTTTTAAAATATGAAGACACGTATAAAACAATTATAATTTGTAGATTCTGAGGTATCTCTAATATTTGTGGTATATAATAATGGTATAATTTTGTGGACTTTACATTTTTGCTTATGATAGTGATGTTACTATTTTATTGTTTATGTATTGATGTGTGTGTGTGTGTTTATATAGTACTAAGATCTTGTCGTTGATAGAAATTTCTGGATCCGTCCCTGGCAGTGAGACTTGGGAGAGCGAACACCGTGTGCATAGATTCCCAAGTCCCCTGTACACAACGTCACGAATTTAAATTATTTTATATTAAAAGTTCACTTCAAATCCCTGTGTTTTGCGGGGAATCAACATTTTATTAGCAACTAGTTAAGTTCCAGTGAGGATTAATATTTTACGATGCGATTTAGTGTTCTCTGGGGATTGAATTTATTATATGCTTAATTTAGCAGAAGGGATGGTTTCTATTCATAGTTTGCTATTGAAACTTGTTCCACTTTCCCATTGCAGGACATAAACGAAGAGTGGATTTCAGACAAAACCCGCTTTTGTTATGATGGTTTGAAGAGGCAGAGGTTAAATGACCCTATGATTCGTGGTCCTGATGGCTGCTTTAAGCCTGTGAACTGGCGTGAAGCCCTTTCTTTGGTAGCAGAGGTTGTGCATCAAGTTAAACCAGAAGAAATTGTTGGAATATCTGGCAAACTTTCTGATGCTGAGTCCATGATTGCACTAAAAGATTTCTTAAACAGGCTGGGGTCAAATAATGTATGGGGCGAAGGAATTGGCGTAAATACTAATGCTGATTTCAGATCAGGATATATTATGAATACTAGCATTGCTGGTCTTGAGAAAGCAGATGCTTTCCTGTTGGTTGGCACCCAGGTATATCTCTAATTTATTTTAGTGTGCAACTTCTCTAGTTTTATGTTATGGTTAACCAATGATTCCTGTTATATATTGAAATGCGATTCCTTTTTAGCCCTTTGGGATTGCACTGGATTTCATAACTGTAGTTTTGGAGATTCTAATTACAACTATTCAACAATAAAACTTATGAAAATTATTGTTTTATTGGATGCGCTCGTCTACTAGTTATGCTGCCATTTTTATTTTTCGTCTTTAAATGCTGCCATCTTTTATGCATTAATATATGGTGTCTGTTTGGTTATAATGTGTTCACTTCCAAATTTGCAGCCACGGGTGGAAGCTGCTATGGTTAATGCTAGAATACGCAAGACTGTCACGGCAAACCAAGCCAAGGTCGGTTACATTGGGCCTGCTACTGATTTCAACTATGATAACGAACATCTTGGTACTGGTCCTCAAACACTTCTTGAAATTGCTGAGGGTCGACACCCATTTTCCAAGACTATTTCAAATGCCAAAAACCCTGTTATCATTGTTGGTGCTGGGATTTTTGAGAGGAAGGATCAGGATGCAATTTTTGCAGCTGTTGAAACCATTGCAAAACAAGGGAATGTCGTCAGATCTGATTGGAATGGCCTTAATGTATTACTTCTCCATGCTGCCCAGGCTGCTGCACTTGACCTTGGACTTGTGCCCCAATCTGAAAAAAGCCTTGAATCTGCAAAGTTTGTATATTTAATGGGAGCTGATGATACAAACTTGGACAAGATCCCGAAGGATGCTTTTGTTGTTTATCAAGGTCACCATGGTGATAAGAGTGTTTATCGTGCTAATGTCATTTTGCCAGCAGCTGCATTCAGTGAGAAGGAAGGTACATATGAAAATACTGAAGGATGCACACAGCAAACATGGCCTGCAGTTCCAACAGTTGGAGACTCCAGAGATGATTGGAAGATAATTCGAGCTCTGTCCGAGATAGCAGGGGTGCGTTTACCCTATGATACAATTGGGGGAGTTCGCGCCCGACTGAGGACTGTAGCGCCAAACCTTGCGCAAATGGACGAGAGAGAACCAGCTGCATTGCCATCTTCACTGAGACCAACCTTCACTAAGAAGGTGGATCCATCACCATTCGGGATTGCGGTTGAGAATTTCTATATGACTGACGCAATTACCAGGGCATCAAAGATAATGGCTCAATGCAGTGCTATGCTGTTGAAGAAATGA
Protein sequence

>MS.gene008303.t1

MGFGLLASKTFRQTTTTTRLLLLRSITTKPELRKPEPAAQPQPEQPLVDLTPRTPVAGARVHFPNPDDAIEVFVDGYPVKIPKGFSVLQACEVAGVDIPRFCYHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTDTPIAKKAREGVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGSDRGRFTDMKRSVVDKNLGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGMLGRGSGEEIGTYVEKLMTSELSGNVIDICPVGALTSKPFAFKARNWELKNTETIDVTDAVGSNIRIDSRGPEVMRIVPRLNEDINEEWISDKTRFCYDGLKRQRLNDPMIRGPDGCFKPVNWREALSLVAEVVHQVKPEEIVGISGKLSDAESMIALKDFLNRLGSNNVWGEGIGVNTNADFRSGYIMNTSIAGLEKADAFLLVGTQPRVEAAMVNARIRKTVTANQAKVGYIGPATDFNYDNEHLGTGPQTLLEIAEGRHPFSKTISNAKNPVIIVGAGIFERKDQDAIFAAVETIAKQGNVVRSDWNGLNVLLLHAAQAAALDLGLVPQSEKSLESAKFVYLMGADDTNLDKIPKDAFVVYQGHHGDKSVYRANVILPAAAFSEKEGTYENTEGCTQQTWPAVPTVGDSRDDWKIIRALSEIAGVRLPYDTIGGVRARLRTVAPNLAQMDEREPAALPSSLRPTFTKKVDPSPFGIAVENFYMTDAITRASKIMAQCSAMLLKK