Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009732.t1 | XP_003615940.1 | 88.1 | 160 | 19 | 0 | 1 | 160 | 1 | 160 | 5.00E-67 | 263.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009732.t1 | G7K5S3 | 88.1 | 160 | 19 | 0 | 1 | 160 | 1 | 160 | 3.6e-67 | 263.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene01564 | MS.gene009732 | PPI |
| MS.gene54297 | MS.gene009732 | PPI |
| MS.gene002580 | MS.gene009732 | PPI |
| MS.gene004251 | MS.gene009732 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009732.t1 | MTR_5g074290 | 97.500 | 160 | 4 | 0 | 1 | 160 | 1 | 160 | 1.64e-112 | 316 |
| MS.gene009732.t1 | MTR_3g031140 | 64.535 | 172 | 45 | 4 | 1 | 158 | 1 | 170 | 4.13e-67 | 201 |
| MS.gene009732.t1 | MTR_2g016150 | 42.778 | 180 | 80 | 5 | 1 | 157 | 1 | 180 | 7.97e-26 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009732.t1 | AT2G30230 | 55.866 | 179 | 56 | 4 | 1 | 159 | 1 | 176 | 1.14e-57 | 178 |
| MS.gene009732.t1 | AT1G06980 | 58.333 | 168 | 61 | 3 | 1 | 159 | 1 | 168 | 1.82e-57 | 177 |
| MS.gene009732.t1 | AT1G29195 | 39.683 | 189 | 81 | 5 | 1 | 157 | 1 | 188 | 2.35e-34 | 119 |
Find 33 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCGTATAGTTCATTTAAA+TGG | 0.184956 | 5.3:+58649068 | MS.gene009732:CDS |
| AGAACTTCCCCAGCTGAAAT+TGG | 0.250090 | 5.3:-58649113 | None:intergenic |
| GAATAGCTTAAGGTGTTGTT+TGG | 0.353238 | 5.3:+58649013 | MS.gene009732:CDS |
| GGTTTGGTTAGAACATGATT+TGG | 0.374435 | 5.3:-58649146 | None:intergenic |
| CGAACGACTCCTTCAGAGCT+TGG | 0.393027 | 5.3:-58649167 | None:intergenic |
| ACAGAAACATTGTGAGTCTA+AGG | 0.406561 | 5.3:+58649385 | MS.gene009732:CDS |
| GATCCCATCCACATCGTTAC+CGG | 0.408720 | 5.3:+58649247 | MS.gene009732:CDS |
| AGCCATTTAAATGAACTATA+CGG | 0.418439 | 5.3:-58649070 | None:intergenic |
| GACTCCTTCAGAGCTTGGTT+TGG | 0.436195 | 5.3:-58649162 | None:intergenic |
| GGTTAGAACATGATTTGGAT+TGG | 0.459615 | 5.3:-58649141 | None:intergenic |
| CAATAATGGGGAATAGCTTA+AGG | 0.463109 | 5.3:+58649003 | None:intergenic |
| ATTACACGTCCAATTTCAGC+TGG | 0.481683 | 5.3:+58649104 | MS.gene009732:CDS |
| ATCGCCGGCATAGCCGTAGC+TGG | 0.494180 | 5.3:+58649429 | MS.gene009732:CDS |
| CAAGATGAGGTTGCCAGCTA+CGG | 0.497711 | 5.3:-58649442 | None:intergenic |
| TTCTCCGGTAACGATGTGGA+TGG | 0.498133 | 5.3:-58649251 | None:intergenic |
| GTTGCCAGCTACGGCTATGC+CGG | 0.501915 | 5.3:-58649433 | None:intergenic |
| CCGGCGAAAGGATGAGAATC+CGG | 0.510917 | 5.3:-58649190 | None:intergenic |
| CTTCTTGCTCTTCTCTTCAG+AGG | 0.524828 | 5.3:-58649336 | None:intergenic |
| CCGGATTCTCATCCTTTCGC+CGG | 0.527436 | 5.3:+58649190 | MS.gene009732:CDS |
| CTAACCAAACCAAGCTCTGA+AGG | 0.531317 | 5.3:+58649158 | MS.gene009732:CDS |
| AAAGCTCGAGGCGAGATCGC+CGG | 0.545398 | 5.3:+58649414 | MS.gene009732:CDS |
| TCTATGATACTTTCAAGATG+AGG | 0.552754 | 5.3:-58649455 | None:intergenic |
| TCTCCGGTAACGATGTGGAT+GGG | 0.553981 | 5.3:-58649250 | None:intergenic |
| GCTCTGAAGGAGTCGTTCGC+CGG | 0.557154 | 5.3:+58649171 | MS.gene009732:CDS |
| TGAGCTCAGTCTCCGGCGAA+AGG | 0.557541 | 5.3:-58649202 | None:intergenic |
| CCTCTCTTGAGCTCAGTCTC+CGG | 0.558760 | 5.3:-58649209 | None:intergenic |
| TTGGCTTGTGTTCTTCCATG+TGG | 0.602379 | 5.3:+58649032 | MS.gene009732:CDS |
| CCGGAGACTGAGCTCAAGAG+AGG | 0.612896 | 5.3:+58649209 | MS.gene009732:CDS |
| TACACGTCCAATTTCAGCTG+GGG | 0.613892 | 5.3:+58649106 | MS.gene009732:CDS |
| TTACACGTCCAATTTCAGCT+GGG | 0.627095 | 5.3:+58649105 | MS.gene009732:CDS |
| ATCAAGTCTAGTGCTCCACA+TGG | 0.636272 | 5.3:-58649047 | None:intergenic |
| CTAAGGAGATGAAAAGCTCG+AGG | 0.669226 | 5.3:+58649402 | MS.gene009732:CDS |
| GAGGGTCAGAAGAAGCGTCG+CGG | 0.726452 | 5.3:+58649280 | MS.gene009732:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGCCATTTAAATGAACTATA+CGG | - | chr5.3:58649073-58649092 | None:intergenic | 25.0% |
| ! | ATCCGTATAGTTCATTTAAA+TGG | + | chr5.3:58649068-58649087 | MS.gene009732:CDS | 25.0% |
| TCTATGATACTTTCAAGATG+AGG | - | chr5.3:58649458-58649477 | None:intergenic | 30.0% | |
| ACAGAAACATTGTGAGTCTA+AGG | + | chr5.3:58649385-58649404 | MS.gene009732:CDS | 35.0% | |
| ! | GGTTAGAACATGATTTGGAT+TGG | - | chr5.3:58649144-58649163 | None:intergenic | 35.0% |
| ! | GGTTTGGTTAGAACATGATT+TGG | - | chr5.3:58649149-58649168 | None:intergenic | 35.0% |
| !! | GAATAGCTTAAGGTGTTGTT+TGG | + | chr5.3:58649013-58649032 | MS.gene009732:CDS | 35.0% |
| ATTACACGTCCAATTTCAGC+TGG | + | chr5.3:58649104-58649123 | MS.gene009732:CDS | 40.0% | |
| CATCGTTACCGGAGAAAAAA+CGG | + | chr5.3:58649258-58649277 | MS.gene009732:CDS | 40.0% | |
| TTACACGTCCAATTTCAGCT+GGG | + | chr5.3:58649105-58649124 | MS.gene009732:CDS | 40.0% | |
| !!! | TTTTTTCTCCGGTAACGATG+TGG | - | chr5.3:58649258-58649277 | None:intergenic | 40.0% |
| AGAACTTCCCCAGCTGAAAT+TGG | - | chr5.3:58649116-58649135 | None:intergenic | 45.0% | |
| ATCAAGTCTAGTGCTCCACA+TGG | - | chr5.3:58649050-58649069 | None:intergenic | 45.0% | |
| CTAAGGAGATGAAAAGCTCG+AGG | + | chr5.3:58649402-58649421 | MS.gene009732:CDS | 45.0% | |
| CTTCTTGCTCTTCTCTTCAG+AGG | - | chr5.3:58649339-58649358 | None:intergenic | 45.0% | |
| GTTACCGGAGAAAAAACGGA+GGG | + | chr5.3:58649262-58649281 | MS.gene009732:CDS | 45.0% | |
| TACACGTCCAATTTCAGCTG+GGG | + | chr5.3:58649106-58649125 | MS.gene009732:CDS | 45.0% | |
| ! | CTAACCAAACCAAGCTCTGA+AGG | + | chr5.3:58649158-58649177 | MS.gene009732:CDS | 45.0% |
| !! | TTGGCTTGTGTTCTTCCATG+TGG | + | chr5.3:58649032-58649051 | MS.gene009732:CDS | 45.0% |
| CAAGATGAGGTTGCCAGCTA+CGG | - | chr5.3:58649445-58649464 | None:intergenic | 50.0% | |
| CGTTACCGGAGAAAAAACGG+AGG | + | chr5.3:58649261-58649280 | MS.gene009732:CDS | 50.0% | |
| GACTCCTTCAGAGCTTGGTT+TGG | - | chr5.3:58649165-58649184 | None:intergenic | 50.0% | |
| GATCCCATCCACATCGTTAC+CGG | + | chr5.3:58649247-58649266 | MS.gene009732:CDS | 50.0% | |
| TCTCCGGTAACGATGTGGAT+GGG | - | chr5.3:58649253-58649272 | None:intergenic | 50.0% | |
| TTCTCCGGTAACGATGTGGA+TGG | - | chr5.3:58649254-58649273 | None:intergenic | 50.0% | |
| !!! | CTGACCCTCCGTTTTTTCTC+CGG | - | chr5.3:58649269-58649288 | None:intergenic | 50.0% |
| CCGGATTCTCATCCTTTCGC+CGG | + | chr5.3:58649190-58649209 | MS.gene009732:CDS | 55.0% | |
| CCGGCGAAAGGATGAGAATC+CGG | - | chr5.3:58649193-58649212 | None:intergenic | 55.0% | |
| CCTCTCTTGAGCTCAGTCTC+CGG | - | chr5.3:58649212-58649231 | None:intergenic | 55.0% | |
| CGAACGACTCCTTCAGAGCT+TGG | - | chr5.3:58649170-58649189 | None:intergenic | 55.0% | |
| AAAGCTCGAGGCGAGATCGC+CGG | + | chr5.3:58649414-58649433 | MS.gene009732:CDS | 60.0% | |
| CCGGAGACTGAGCTCAAGAG+AGG | + | chr5.3:58649209-58649228 | MS.gene009732:CDS | 60.0% | |
| GTTGCCAGCTACGGCTATGC+CGG | - | chr5.3:58649436-58649455 | None:intergenic | 60.0% | |
| TGAGCTCAGTCTCCGGCGAA+AGG | - | chr5.3:58649205-58649224 | None:intergenic | 60.0% | |
| !! | GAGGGTCAGAAGAAGCGTCG+CGG | + | chr5.3:58649280-58649299 | MS.gene009732:CDS | 60.0% |
| !! | GCTCTGAAGGAGTCGTTCGC+CGG | + | chr5.3:58649171-58649190 | MS.gene009732:CDS | 60.0% |
| ATCGCCGGCATAGCCGTAGC+TGG | + | chr5.3:58649429-58649448 | MS.gene009732:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 58649008 | 58649490 | 58649008 | ID=MS.gene009732 |
| chr5.3 | mRNA | 58649008 | 58649490 | 58649008 | ID=MS.gene009732.t1;Parent=MS.gene009732 |
| chr5.3 | exon | 58649008 | 58649490 | 58649008 | ID=MS.gene009732.t1.exon1;Parent=MS.gene009732.t1 |
| chr5.3 | CDS | 58649008 | 58649490 | 58649008 | ID=cds.MS.gene009732.t1;Parent=MS.gene009732.t1 |
| Gene Sequence |
| Protein sequence |
