Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013029.t1 | XP_003602973.1 | 79.9 | 383 | 75 | 1 | 43 | 425 | 9 | 389 | 9.90E-171 | 609.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013029.t1 | Q9S7T8 | 53.7 | 382 | 174 | 3 | 44 | 423 | 10 | 390 | 1.1e-114 | 414.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013029.t1 | G7J5Q8 | 79.9 | 383 | 75 | 1 | 43 | 425 | 9 | 389 | 7.1e-171 | 609.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene031697 | MS.gene013029 | PPI |
| MS.gene065945 | MS.gene013029 | PPI |
| MS.gene013029 | MS.gene045585 | PPI |
| MS.gene013029 | MS.gene065944 | PPI |
| MS.gene006610 | MS.gene013029 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013029.t1 | MTR_3g101030 | 73.522 | 423 | 91 | 5 | 22 | 425 | 9 | 429 | 0.0 | 606 |
| MS.gene013029.t1 | MTR_3g101050 | 76.941 | 425 | 69 | 7 | 1 | 424 | 1 | 397 | 0.0 | 602 |
| MS.gene013029.t1 | MTR_3g101010 | 79.896 | 383 | 75 | 1 | 43 | 425 | 9 | 389 | 0.0 | 599 |
| MS.gene013029.t1 | MTR_3g018740 | 72.063 | 383 | 102 | 3 | 42 | 423 | 8 | 386 | 0.0 | 536 |
| MS.gene013029.t1 | MTR_8g027395 | 68.217 | 387 | 117 | 2 | 39 | 425 | 17 | 397 | 0.0 | 521 |
| MS.gene013029.t1 | MTR_4g045707 | 66.667 | 390 | 126 | 3 | 36 | 425 | 3 | 388 | 1.01e-180 | 509 |
| MS.gene013029.t1 | MTR_8g027420 | 66.408 | 387 | 113 | 3 | 39 | 425 | 6 | 375 | 1.32e-179 | 506 |
| MS.gene013029.t1 | MTR_8g461160 | 65.796 | 383 | 129 | 1 | 43 | 423 | 10 | 392 | 2.29e-177 | 501 |
| MS.gene013029.t1 | MTR_3g101180 | 63.750 | 400 | 129 | 3 | 36 | 423 | 3 | 398 | 9.56e-176 | 497 |
| MS.gene013029.t1 | MTR_7g451890 | 63.896 | 385 | 137 | 1 | 43 | 425 | 18 | 402 | 5.23e-175 | 495 |
| MS.gene013029.t1 | MTR_7g047780 | 63.731 | 386 | 136 | 2 | 43 | 425 | 18 | 402 | 1.39e-172 | 489 |
| MS.gene013029.t1 | MTR_7g050750 | 63.636 | 385 | 138 | 1 | 43 | 425 | 12 | 396 | 1.76e-172 | 488 |
| MS.gene013029.t1 | MTR_2g046030 | 65.026 | 386 | 124 | 4 | 42 | 423 | 8 | 386 | 7.90e-171 | 484 |
| MS.gene013029.t1 | MTR_4g088525 | 65.583 | 369 | 123 | 1 | 56 | 424 | 4 | 368 | 8.72e-171 | 483 |
| MS.gene013029.t1 | MTR_3g101130 | 63.542 | 384 | 135 | 2 | 43 | 425 | 10 | 389 | 5.44e-170 | 482 |
| MS.gene013029.t1 | MTR_2g045980 | 65.285 | 386 | 123 | 4 | 42 | 423 | 8 | 386 | 4.65e-169 | 479 |
| MS.gene013029.t1 | MTR_7g050810 | 62.597 | 385 | 142 | 1 | 43 | 425 | 20 | 404 | 6.28e-169 | 479 |
| MS.gene013029.t1 | MTR_4g088535 | 65.885 | 384 | 109 | 4 | 37 | 420 | 5 | 366 | 3.95e-167 | 474 |
| MS.gene013029.t1 | MTR_3g048000 | 63.969 | 383 | 115 | 5 | 43 | 424 | 31 | 391 | 2.92e-166 | 472 |
| MS.gene013029.t1 | MTR_3g015620 | 62.176 | 386 | 116 | 6 | 42 | 423 | 19 | 378 | 1.65e-152 | 437 |
| MS.gene013029.t1 | MTR_3g102920 | 60.526 | 380 | 110 | 6 | 47 | 423 | 66 | 408 | 5.17e-145 | 419 |
| MS.gene013029.t1 | MTR_3g100995 | 74.016 | 254 | 63 | 2 | 172 | 425 | 14 | 264 | 7.33e-136 | 390 |
| MS.gene013029.t1 | MTR_3g101200 | 57.143 | 371 | 132 | 5 | 55 | 423 | 45 | 390 | 1.20e-133 | 389 |
| MS.gene013029.t1 | MTR_3g101190 | 55.342 | 365 | 96 | 3 | 63 | 425 | 13 | 312 | 9.96e-131 | 379 |
| MS.gene013029.t1 | MTR_3g018747 | 69.231 | 260 | 64 | 3 | 165 | 423 | 9 | 253 | 4.25e-121 | 352 |
| MS.gene013029.t1 | MTR_7g050830 | 55.844 | 308 | 105 | 2 | 45 | 332 | 22 | 318 | 2.99e-113 | 335 |
| MS.gene013029.t1 | MTR_3g100010 | 56.604 | 318 | 90 | 2 | 62 | 346 | 38 | 340 | 2.42e-112 | 333 |
| MS.gene013029.t1 | MTR_3g015760 | 64.727 | 275 | 93 | 2 | 150 | 423 | 8 | 279 | 1.52e-111 | 329 |
| MS.gene013029.t1 | MTR_3g111160 | 70.952 | 210 | 58 | 2 | 217 | 425 | 13 | 220 | 7.74e-95 | 284 |
| MS.gene013029.t1 | MTR_3g101110 | 61.317 | 243 | 64 | 4 | 165 | 403 | 68 | 284 | 2.32e-90 | 275 |
| MS.gene013029.t1 | MTR_3g100520 | 62.613 | 222 | 68 | 3 | 206 | 425 | 1 | 209 | 9.71e-89 | 268 |
| MS.gene013029.t1 | MTR_3g114310 | 41.500 | 400 | 209 | 11 | 41 | 425 | 21 | 410 | 1.37e-86 | 270 |
| MS.gene013029.t1 | MTR_3g099970 | 66.327 | 196 | 49 | 2 | 165 | 360 | 20 | 198 | 6.92e-86 | 260 |
| MS.gene013029.t1 | MTR_2g046410 | 66.010 | 203 | 63 | 2 | 225 | 423 | 147 | 347 | 9.20e-86 | 266 |
| MS.gene013029.t1 | MTR_3g101020 | 64.171 | 187 | 40 | 2 | 239 | 425 | 1 | 160 | 8.05e-76 | 233 |
| MS.gene013029.t1 | MTR_3g101150 | 70.253 | 158 | 43 | 2 | 271 | 424 | 1 | 158 | 2.28e-70 | 219 |
| MS.gene013029.t1 | MTR_3g102870 | 64.286 | 154 | 55 | 0 | 228 | 381 | 1 | 154 | 1.89e-63 | 202 |
| MS.gene013029.t1 | MTR_3g111150 | 59.551 | 178 | 46 | 3 | 42 | 219 | 2 | 153 | 1.31e-62 | 199 |
| MS.gene013029.t1 | MTR_3g100990 | 58.784 | 148 | 58 | 2 | 43 | 190 | 9 | 153 | 1.85e-45 | 164 |
| MS.gene013029.t1 | MTR_4g093550 | 69.512 | 82 | 23 | 1 | 43 | 124 | 10 | 89 | 5.82e-28 | 106 |
| MS.gene013029.t1 | MTR_3g101140 | 47.581 | 124 | 47 | 4 | 304 | 425 | 9 | 116 | 1.45e-20 | 87.0 |
| MS.gene013029.t1 | MTR_6g046890 | 63.158 | 76 | 25 | 1 | 57 | 132 | 15 | 87 | 1.62e-20 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013029.t1 | AT1G47710 | 53.665 | 382 | 174 | 3 | 44 | 423 | 10 | 390 | 2.25e-145 | 419 |
| MS.gene013029.t1 | AT1G47710 | 53.665 | 382 | 174 | 3 | 44 | 423 | 37 | 417 | 9.44e-145 | 419 |
| MS.gene013029.t1 | AT3G45220 | 48.731 | 394 | 176 | 6 | 43 | 422 | 9 | 390 | 2.34e-126 | 371 |
| MS.gene013029.t1 | AT2G25240 | 49.740 | 384 | 179 | 7 | 43 | 422 | 9 | 382 | 1.25e-122 | 361 |
| MS.gene013029.t1 | AT2G26390 | 48.849 | 391 | 176 | 7 | 43 | 422 | 9 | 386 | 8.61e-121 | 357 |
| MS.gene013029.t1 | AT2G14540 | 45.312 | 384 | 183 | 6 | 43 | 422 | 37 | 397 | 2.11e-97 | 298 |
| MS.gene013029.t1 | AT1G64030 | 43.264 | 386 | 190 | 6 | 43 | 422 | 9 | 371 | 8.82e-97 | 295 |
| MS.gene013029.t1 | AT1G62170 | 42.162 | 370 | 183 | 5 | 62 | 424 | 88 | 433 | 9.53e-96 | 294 |
| MS.gene013029.t1 | AT2G35580 | 44.000 | 375 | 179 | 9 | 58 | 422 | 18 | 371 | 3.23e-91 | 280 |
| MS.gene013029.t1 | AT1G62170 | 38.710 | 403 | 183 | 6 | 62 | 424 | 88 | 466 | 1.08e-88 | 277 |
| MS.gene013029.t1 | AT1G64010 | 45.872 | 218 | 94 | 4 | 206 | 422 | 1 | 195 | 5.41e-58 | 189 |
| MS.gene013029.t1 | AT1G64010 | 45.813 | 203 | 86 | 4 | 221 | 422 | 2 | 181 | 8.26e-52 | 172 |
| MS.gene013029.t1 | AT1G63280 | 54.783 | 115 | 48 | 2 | 165 | 277 | 8 | 120 | 2.70e-35 | 127 |
| MS.gene013029.t1 | AT1G62160 | 37.129 | 202 | 99 | 6 | 228 | 424 | 42 | 220 | 1.17e-31 | 120 |
| MS.gene013029.t1 | AT1G51330 | 49.550 | 111 | 53 | 2 | 164 | 271 | 30 | 140 | 4.44e-25 | 102 |
| MS.gene013029.t1 | AT1G64020 | 35.088 | 114 | 71 | 3 | 44 | 156 | 10 | 121 | 1.13e-15 | 73.6 |
Find 99 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGACTTGTGGAATATTT+TGG | 0.106119 | 3.3:-83734886 | None:intergenic |
| GCTAATGCACTATACTTTAA+AGG | 0.155210 | 3.3:+83734426 | MS.gene013029:CDS |
| CGAGAAAAGAAGTGAATTTA+TGG | 0.176628 | 3.3:+83734325 | MS.gene013029:CDS |
| TGAAACTTCTGATATGTTAA+AGG | 0.194021 | 3.3:+83734782 | MS.gene013029:CDS |
| TTATCTTCACCTTGCTTATA+AGG | 0.268250 | 3.3:-83734594 | None:intergenic |
| CTTGTCTTGATGAGATTCTT+TGG | 0.271973 | 3.3:-83733831 | None:intergenic |
| GACGGTGGCTGCAGCAGCTT+TGG | 0.307932 | 3.3:-83734931 | None:intergenic |
| AAGTGGAAGTAGGTGACTTT+AGG | 0.323594 | 3.3:+83734730 | MS.gene013029:CDS |
| GAGGAAGTCAAGGGCGTATT+CGG | 0.323880 | 3.3:-83733747 | None:intergenic |
| GCCAACTTTGCTTGAGAAGC+TGG | 0.324647 | 3.3:+83734665 | MS.gene013029:CDS |
| ATTCAAAAGGTGAAAGTCAT+AGG | 0.334305 | 3.3:-83734487 | None:intergenic |
| TTTCTTCCAAATGCAAAAGA+TGG | 0.344851 | 3.3:+83734639 | MS.gene013029:CDS |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.350146 | 3.3:+83735071 | MS.gene013029:CDS |
| TTCTTTGGAGAAAAGATGTT+TGG | 0.357142 | 3.3:-83733816 | None:intergenic |
| GAATGGAGGGTTGACTAAAA+TGG | 0.363840 | 3.3:+83734830 | MS.gene013029:CDS |
| GGTTGTTGGCGGGTTGGAAT+CGG | 0.375878 | 3.3:-83733769 | None:intergenic |
| ACCGGAACTATCGCCTTTGT+TGG | 0.400356 | 3.3:+83735047 | MS.gene013029:CDS |
| CAACAACTTCTCGACTTCCT+TGG | 0.417102 | 3.3:+83733929 | MS.gene013029:CDS |
| TGAAAGAAGGTTGAAGAGAA+AGG | 0.426729 | 3.3:-83734075 | None:intergenic |
| AACTATCGCCTTTGTTGGGC+AGG | 0.426929 | 3.3:+83735052 | MS.gene013029:CDS |
| ATTAAAGAACTTCTTCCTCT+AGG | 0.427888 | 3.3:+83734372 | MS.gene013029:CDS |
| CCCAACAAAGGCGATAGTTC+CGG | 0.429621 | 3.3:-83735048 | None:intergenic |
| GGAAACGTTGCTTTGGTTGT+TGG | 0.432490 | 3.3:-83733783 | None:intergenic |
| GACACAATTTGCTTGAAAGA+AGG | 0.436343 | 3.3:-83734088 | None:intergenic |
| GATAATGAGTGGGATCGATT+TGG | 0.439052 | 3.3:-83733705 | None:intergenic |
| TCTCTGATCATAAATAAGAA+TGG | 0.440676 | 3.3:-83735017 | None:intergenic |
| ACCGTCGCAACTTTACTTTA+CGG | 0.440844 | 3.3:+83734948 | MS.gene013029:CDS |
| TAACTGAGTTTCCATTCAAA+AGG | 0.441115 | 3.3:-83734500 | None:intergenic |
| AACGTTGCTTTGGTTGTTGG+CGG | 0.442357 | 3.3:-83733780 | None:intergenic |
| TTTGAATGGAAACTCAGTTA+AGG | 0.443112 | 3.3:+83734503 | MS.gene013029:CDS |
| TTAAAGAACTTCTTCCTCTA+GGG | 0.444956 | 3.3:+83734373 | MS.gene013029:CDS |
| CTCTGATCATAAATAAGAAT+GGG | 0.447141 | 3.3:-83735016 | None:intergenic |
| CAAGCTTCCTCTTGATAAAG+TGG | 0.447424 | 3.3:+83734713 | MS.gene013029:CDS |
| GAGAAAAGAAGTGAATTTAT+GGG | 0.455502 | 3.3:+83734326 | MS.gene013029:CDS |
| TTCATTGAAGTAAATGAAGA+AGG | 0.457204 | 3.3:+83734906 | MS.gene013029:CDS |
| GAAAGAAGGTTGAAGAGAAA+GGG | 0.460189 | 3.3:-83734074 | None:intergenic |
| TGCTTTGGTTGTTGGCGGGT+TGG | 0.465518 | 3.3:-83733775 | None:intergenic |
| CTTCTTCTCACTGGTCATGA+AGG | 0.470421 | 3.3:-83734529 | None:intergenic |
| CCGGAACTATCGCCTTTGTT+GGG | 0.480048 | 3.3:+83735048 | MS.gene013029:CDS |
| CGATGATGCTGAGCACAACT+TGG | 0.482600 | 3.3:-83733880 | None:intergenic |
| AAAGACGGTGCTCCCGCAGG+TGG | 0.483007 | 3.3:+83734010 | MS.gene013029:CDS |
| TGGTGAGGGAAACGTTGCTT+TGG | 0.486728 | 3.3:-83733790 | None:intergenic |
| TTCTTCCAAATGCAAAAGAT+GGG | 0.486946 | 3.3:+83734640 | MS.gene013029:CDS |
| GCCAGCTTCTCAAGCAAAGT+TGG | 0.489801 | 3.3:-83734666 | None:intergenic |
| GAAGAAGTAGAAAACAGCTT+TGG | 0.494264 | 3.3:-83733623 | None:intergenic |
| GTGTTGTAGTAACTCAGATT+CGG | 0.496759 | 3.3:-83734691 | None:intergenic |
| CTTCACCTTGCTTATAAGGA+AGG | 0.497179 | 3.3:-83734590 | None:intergenic |
| GAGAGACGAGGCCCACCTGC+GGG | 0.498754 | 3.3:-83734022 | None:intergenic |
| TCTGATATGTTAAAGGAGCT+TGG | 0.499230 | 3.3:+83734789 | MS.gene013029:CDS |
| TTACTTCAATGAAAGACTTG+TGG | 0.504558 | 3.3:-83734896 | None:intergenic |
| CATCATCGCTGATGGTTCTG+AGG | 0.506455 | 3.3:+83733895 | MS.gene013029:CDS |
| GGGAGCACCGTCTTTGAGAA+TGG | 0.518279 | 3.3:-83734002 | None:intergenic |
| CTCAAAGACGGTGCTCCCGC+AGG | 0.518597 | 3.3:+83734007 | MS.gene013029:CDS |
| AATAAACTGCTTCTTCTCAC+TGG | 0.523013 | 3.3:-83734538 | None:intergenic |
| TCAAATGAAATATTGAACCT+TGG | 0.524127 | 3.3:-83734756 | None:intergenic |
| AAAGATGTTTGGCGATGGTG+AGG | 0.524270 | 3.3:-83733805 | None:intergenic |
| GCTACAAAGTCTATGTCTGT+TGG | 0.529202 | 3.3:-83734987 | None:intergenic |
| TAGGTGACTTTAGGATTCCA+AGG | 0.533810 | 3.3:+83734739 | MS.gene013029:CDS |
| GAAGAAGTTTAGATGATCGA+TGG | 0.536342 | 3.3:-83733960 | None:intergenic |
| TTCTGTTGATTTCAAGAACA+AGG | 0.537281 | 3.3:+83734135 | MS.gene013029:CDS |
| ATGATCAGAGAAGATTTGAC+CGG | 0.538023 | 3.3:+83735029 | MS.gene013029:CDS |
| TGTTGGCGGGTTGGAATCGG+AGG | 0.542527 | 3.3:-83733766 | None:intergenic |
| GTTGGAATCGGAGGAAGTCA+AGG | 0.547443 | 3.3:-83733757 | None:intergenic |
| ACGTTGCTTTGGTTGTTGGC+GGG | 0.547721 | 3.3:-83733779 | None:intergenic |
| GTCAACCCTCCATTCGTGAA+AGG | 0.553572 | 3.3:-83734822 | None:intergenic |
| CACTATACTTTAAAGGAGAG+TGG | 0.554157 | 3.3:+83734433 | MS.gene013029:CDS |
| GTGCTCAGCATCATCGCTGA+TGG | 0.561012 | 3.3:+83733887 | MS.gene013029:CDS |
| AAGACGGTGCTCCCGCAGGT+GGG | 0.563207 | 3.3:+83734011 | MS.gene013029:CDS |
| CCTACTTCCACTTTATCAAG+AGG | 0.564451 | 3.3:-83734720 | None:intergenic |
| CCTCTTGATAAAGTGGAAGT+AGG | 0.566916 | 3.3:+83734720 | MS.gene013029:CDS |
| CTTGGCCTTCCTTATAAGCA+AGG | 0.569395 | 3.3:+83734585 | MS.gene013029:CDS |
| TTCTTCTCACTGGTCATGAA+GGG | 0.569786 | 3.3:-83734528 | None:intergenic |
| GAGTCGATGTTGATAATGAG+TGG | 0.573168 | 3.3:-83733716 | None:intergenic |
| TGGGCTGAAAGAGAGACAAA+TGG | 0.574537 | 3.3:+83734345 | MS.gene013029:CDS |
| GTCGAGAAGTTGTTGTTGAG+TGG | 0.576826 | 3.3:-83733921 | None:intergenic |
| TGCTAAGATCATCCAGCAAG+AGG | 0.578961 | 3.3:-83735083 | None:intergenic |
| AGAGAGACGAGGCCCACCTG+CGG | 0.584963 | 3.3:-83734023 | None:intergenic |
| ATCATCGCTGATGGTTCTGA+GGG | 0.591707 | 3.3:+83733896 | MS.gene013029:CDS |
| GTAAAGTAAAGTTGCGACGG+TGG | 0.595397 | 3.3:-83734946 | None:intergenic |
| AAGATGTTTGGCGATGGTGA+GGG | 0.596429 | 3.3:-83733804 | None:intergenic |
| ATTGAGCACCTGCCCAACAA+AGG | 0.599203 | 3.3:-83735060 | None:intergenic |
| GTAGAAAACAGCTTTGGCAA+TGG | 0.604998 | 3.3:-83733617 | None:intergenic |
| TTGGAATCGGAGGAAGTCAA+GGG | 0.608158 | 3.3:-83733756 | None:intergenic |
| CAAGGGCGTATTCGGATCGG+AGG | 0.609828 | 3.3:-83733739 | None:intergenic |
| TATGTCTGTTGGAAGACTCA+AGG | 0.610434 | 3.3:-83734976 | None:intergenic |
| AGAACTTCTTCCTCTAGGGT+CGG | 0.613652 | 3.3:+83734377 | MS.gene013029:CDS |
| AGTCAAGGGCGTATTCGGAT+CGG | 0.617677 | 3.3:-83733742 | None:intergenic |
| TGATCATAAATAAGAATGGG+TGG | 0.618025 | 3.3:-83735013 | None:intergenic |
| GTCTTCGCCATTCTCAAAGA+CGG | 0.621526 | 3.3:+83733995 | MS.gene013029:CDS |
| TCCGTAAAGTAAAGTTGCGA+CGG | 0.626090 | 3.3:-83734949 | None:intergenic |
| AGTCGATGTTGATAATGAGT+GGG | 0.634885 | 3.3:-83733715 | None:intergenic |
| TATGTTAAAGGAGCTTGGCG+TGG | 0.637410 | 3.3:+83734794 | MS.gene013029:CDS |
| CTTGCTTATAAGGAAGGCCA+AGG | 0.645897 | 3.3:-83734584 | None:intergenic |
| GGAGAAAAGATGTTTGGCGA+TGG | 0.656877 | 3.3:-83733810 | None:intergenic |
| AAGGGCGTATTCGGATCGGA+GGG | 0.661792 | 3.3:-83733738 | None:intergenic |
| GATCGATGGATTTAGAGCCA+AGG | 0.665025 | 3.3:-83733946 | None:intergenic |
| CCATCGACAAAAGAGAGACG+AGG | 0.674638 | 3.3:-83734034 | None:intergenic |
| AAGTTGTCGACCGACCCTAG+AGG | 0.702735 | 3.3:-83734387 | None:intergenic |
| AGCACAACTTGGATTGACAG+TGG | 0.707392 | 3.3:-83733869 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGATTTTAATTTTTGATA+TGG | + | chr3.3:83734284-83734303 | MS.gene013029:intron | 10.0% |
| !!! | AATTTTTGATATGGTATTAA+AGG | + | chr3.3:83734293-83734312 | MS.gene013029:intron | 15.0% |
| !! | ACAAAATCAAAATCAAAATC+AGG | - | chr3.3:83734209-83734228 | None:intergenic | 20.0% |
| !! | AGAACTCAAAACTATATATT+TGG | + | chr3.3:83734258-83734277 | MS.gene013029:intron | 20.0% |
| !! | CAAAATCAAAATCAAAATCA+GGG | - | chr3.3:83734208-83734227 | None:intergenic | 20.0% |
| ! | ATATCTTGGGAAAAATGAAT+TGG | - | chr3.3:83733685-83733704 | None:intergenic | 25.0% |
| ! | CTCTGATCATAAATAAGAAT+GGG | - | chr3.3:83735019-83735038 | None:intergenic | 25.0% |
| ! | GAGAAAAGAAGTGAATTTAT+GGG | + | chr3.3:83734326-83734345 | MS.gene013029:CDS | 25.0% |
| ! | TCAAATGAAATATTGAACCT+TGG | - | chr3.3:83734759-83734778 | None:intergenic | 25.0% |
| ! | TCTCTGATCATAAATAAGAA+TGG | - | chr3.3:83735020-83735039 | None:intergenic | 25.0% |
| ! | TGAAACTTCTGATATGTTAA+AGG | + | chr3.3:83734782-83734801 | MS.gene013029:CDS | 25.0% |
| ! | TTCATTGAAGTAAATGAAGA+AGG | + | chr3.3:83734906-83734925 | MS.gene013029:CDS | 25.0% |
| !!! | GAATGATAATTTTGATGCTT+CGG | + | chr3.3:83734455-83734474 | MS.gene013029:CDS | 25.0% |
| !!! | GGAATATTTTGGAAACATAA+AGG | - | chr3.3:83734878-83734897 | None:intergenic | 25.0% |
| ATTAAAGAACTTCTTCCTCT+AGG | + | chr3.3:83734372-83734391 | MS.gene013029:CDS | 30.0% | |
| ATTCAAAAGGTGAAAGTCAT+AGG | - | chr3.3:83734490-83734509 | None:intergenic | 30.0% | |
| CGAGAAAAGAAGTGAATTTA+TGG | + | chr3.3:83734325-83734344 | MS.gene013029:CDS | 30.0% | |
| GCTAATGCACTATACTTTAA+AGG | + | chr3.3:83734426-83734445 | MS.gene013029:CDS | 30.0% | |
| TAACTGAGTTTCCATTCAAA+AGG | - | chr3.3:83734503-83734522 | None:intergenic | 30.0% | |
| TGATCATAAATAAGAATGGG+TGG | - | chr3.3:83735016-83735035 | None:intergenic | 30.0% | |
| TTAAAGAACTTCTTCCTCTA+GGG | + | chr3.3:83734373-83734392 | MS.gene013029:CDS | 30.0% | |
| TTACTTCAATGAAAGACTTG+TGG | - | chr3.3:83734899-83734918 | None:intergenic | 30.0% | |
| TTATCTTCACCTTGCTTATA+AGG | - | chr3.3:83734597-83734616 | None:intergenic | 30.0% | |
| TTCTTCCAAATGCAAAAGAT+GGG | + | chr3.3:83734640-83734659 | MS.gene013029:CDS | 30.0% | |
| TTTCTTCCAAATGCAAAAGA+TGG | + | chr3.3:83734639-83734658 | MS.gene013029:CDS | 30.0% | |
| TTTGAATGGAAACTCAGTTA+AGG | + | chr3.3:83734503-83734522 | MS.gene013029:CDS | 30.0% | |
| ! | GAAAGACTTGTGGAATATTT+TGG | - | chr3.3:83734889-83734908 | None:intergenic | 30.0% |
| ! | TATGACTTTCACCTTTTGAA+TGG | + | chr3.3:83734489-83734508 | MS.gene013029:CDS | 30.0% |
| ! | TGTATTTTGTAGCGAAAAGA+GGG | - | chr3.3:83734177-83734196 | None:intergenic | 30.0% |
| ! | TTCTTTGGAGAAAAGATGTT+TGG | - | chr3.3:83733819-83733838 | None:intergenic | 30.0% |
| ! | TTGTATTTTGTAGCGAAAAG+AGG | - | chr3.3:83734178-83734197 | None:intergenic | 30.0% |
| !! | TTCTGTTGATTTCAAGAACA+AGG | + | chr3.3:83734135-83734154 | MS.gene013029:CDS | 30.0% |
| !!! | TTCGATGATTTTAAAGTCCT+TGG | + | chr3.3:83734567-83734586 | MS.gene013029:CDS | 30.0% |
| AATAAACTGCTTCTTCTCAC+TGG | - | chr3.3:83734541-83734560 | None:intergenic | 35.0% | |
| ATGATCAGAGAAGATTTGAC+CGG | + | chr3.3:83735029-83735048 | MS.gene013029:CDS | 35.0% | |
| CTTGTCTTGATGAGATTCTT+TGG | - | chr3.3:83733834-83733853 | None:intergenic | 35.0% | |
| GAAAGAAGGTTGAAGAGAAA+GGG | - | chr3.3:83734077-83734096 | None:intergenic | 35.0% | |
| GAAGAAGTAGAAAACAGCTT+TGG | - | chr3.3:83733626-83733645 | None:intergenic | 35.0% | |
| GAAGAAGTTTAGATGATCGA+TGG | - | chr3.3:83733963-83733982 | None:intergenic | 35.0% | |
| GTGTTGTAGTAACTCAGATT+CGG | - | chr3.3:83734694-83734713 | None:intergenic | 35.0% | |
| TCTGATATGTTAAAGGAGCT+TGG | + | chr3.3:83734789-83734808 | MS.gene013029:CDS | 35.0% | |
| TGAAAGAAGGTTGAAGAGAA+AGG | - | chr3.3:83734078-83734097 | None:intergenic | 35.0% | |
| ! | CACTATACTTTAAAGGAGAG+TGG | + | chr3.3:83734433-83734452 | MS.gene013029:CDS | 35.0% |
| ! | GACACAATTTGCTTGAAAGA+AGG | - | chr3.3:83734091-83734110 | None:intergenic | 35.0% |
| ! | GTATTTTGTAGCGAAAAGAG+GGG | - | chr3.3:83734176-83734195 | None:intergenic | 35.0% |
| ! | TTTTACCTTTCACGAATGGA+GGG | + | chr3.3:83734817-83734836 | MS.gene013029:CDS | 35.0% |
| !! | AGTCGATGTTGATAATGAGT+GGG | - | chr3.3:83733718-83733737 | None:intergenic | 35.0% |
| !!! | ACATAAAGGTTTTGACTCAC+AGG | - | chr3.3:83734864-83734883 | None:intergenic | 35.0% |
| AAGTGGAAGTAGGTGACTTT+AGG | + | chr3.3:83734730-83734749 | MS.gene013029:CDS | 40.0% | |
| ACCGTCGCAACTTTACTTTA+CGG | + | chr3.3:83734948-83734967 | MS.gene013029:CDS | 40.0% | |
| CAAGCTTCCTCTTGATAAAG+TGG | + | chr3.3:83734713-83734732 | MS.gene013029:CDS | 40.0% | |
| CCTACTTCCACTTTATCAAG+AGG | - | chr3.3:83734723-83734742 | None:intergenic | 40.0% | |
| CCTCTTGATAAAGTGGAAGT+AGG | + | chr3.3:83734720-83734739 | MS.gene013029:CDS | 40.0% | |
| CTTCACCTTGCTTATAAGGA+AGG | - | chr3.3:83734593-83734612 | None:intergenic | 40.0% | |
| GAATGGAGGGTTGACTAAAA+TGG | + | chr3.3:83734830-83734849 | MS.gene013029:CDS | 40.0% | |
| GATAATGAGTGGGATCGATT+TGG | - | chr3.3:83733708-83733727 | None:intergenic | 40.0% | |
| GCTACAAAGTCTATGTCTGT+TGG | - | chr3.3:83734990-83735009 | None:intergenic | 40.0% | |
| GGGAAAAATGAATTGGACCA+AGG | - | chr3.3:83733678-83733697 | None:intergenic | 40.0% | |
| TATGTCTGTTGGAAGACTCA+AGG | - | chr3.3:83734979-83734998 | None:intergenic | 40.0% | |
| TCCGTAAAGTAAAGTTGCGA+CGG | - | chr3.3:83734952-83734971 | None:intergenic | 40.0% | |
| TTCTTCTCACTGGTCATGAA+GGG | - | chr3.3:83734531-83734550 | None:intergenic | 40.0% | |
| ! | GTAGAAAACAGCTTTGGCAA+TGG | - | chr3.3:83733620-83733639 | None:intergenic | 40.0% |
| ! | GTTTTACCTTTCACGAATGG+AGG | + | chr3.3:83734816-83734835 | MS.gene013029:CDS | 40.0% |
| !! | GAGTCGATGTTGATAATGAG+TGG | - | chr3.3:83733719-83733738 | None:intergenic | 40.0% |
| !! | GGGATCGATTTGGATATCTT+GGG | - | chr3.3:83733698-83733717 | None:intergenic | 40.0% |
| !! | GTGGCTTGTTTATTCATCCT+TGG | + | chr3.3:83733658-83733677 | MS.gene013029:intron | 40.0% |
| !! | GTGGTTTTACCTTTCACGAA+TGG | + | chr3.3:83734813-83734832 | MS.gene013029:CDS | 40.0% |
| !! | TAGGTGACTTTAGGATTCCA+AGG | + | chr3.3:83734739-83734758 | MS.gene013029:CDS | 40.0% |
| !! | TGGGATCGATTTGGATATCT+TGG | - | chr3.3:83733699-83733718 | None:intergenic | 40.0% |
| AAAGATGTTTGGCGATGGTG+AGG | - | chr3.3:83733808-83733827 | None:intergenic | 45.0% | |
| AAGATGTTTGGCGATGGTGA+GGG | - | chr3.3:83733807-83733826 | None:intergenic | 45.0% | |
| AGCACAACTTGGATTGACAG+TGG | - | chr3.3:83733872-83733891 | None:intergenic | 45.0% | |
| CAACAACTTCTCGACTTCCT+TGG | + | chr3.3:83733929-83733948 | MS.gene013029:CDS | 45.0% | |
| CTTCTTCATGCTCAACATCG+TGG | + | chr3.3:83733639-83733658 | MS.gene013029:intron | 45.0% | |
| CTTCTTCTCACTGGTCATGA+AGG | - | chr3.3:83734532-83734551 | None:intergenic | 45.0% | |
| CTTGCTTATAAGGAAGGCCA+AGG | - | chr3.3:83734587-83734606 | None:intergenic | 45.0% | |
| GGAGAAAAGATGTTTGGCGA+TGG | - | chr3.3:83733813-83733832 | None:intergenic | 45.0% | |
| GTAAAGTAAAGTTGCGACGG+TGG | - | chr3.3:83734949-83734968 | None:intergenic | 45.0% | |
| GTCTTCGCCATTCTCAAAGA+CGG | + | chr3.3:83733995-83734014 | MS.gene013029:CDS | 45.0% | |
| TATGTTAAAGGAGCTTGGCG+TGG | + | chr3.3:83734794-83734813 | MS.gene013029:CDS | 45.0% | |
| TGGGCTGAAAGAGAGACAAA+TGG | + | chr3.3:83734345-83734364 | MS.gene013029:CDS | 45.0% | |
| TTGGAATCGGAGGAAGTCAA+GGG | - | chr3.3:83733759-83733778 | None:intergenic | 45.0% | |
| ! | AACGTTGCTTTGGTTGTTGG+CGG | - | chr3.3:83733783-83733802 | None:intergenic | 45.0% |
| ! | CTCTTTTGTCGATGGTGTGT+GGG | + | chr3.3:83734042-83734061 | MS.gene013029:CDS | 45.0% |
| ! | CTTGGCCTTCCTTATAAGCA+AGG | + | chr3.3:83734585-83734604 | MS.gene013029:CDS | 45.0% |
| ! | GATCGATGGATTTAGAGCCA+AGG | - | chr3.3:83733949-83733968 | None:intergenic | 45.0% |
| ! | GGAAACGTTGCTTTGGTTGT+TGG | - | chr3.3:83733786-83733805 | None:intergenic | 45.0% |
| ! | GTCGAGAAGTTGTTGTTGAG+TGG | - | chr3.3:83733924-83733943 | None:intergenic | 45.0% |
| ! | TCTCTTTTGTCGATGGTGTG+TGG | + | chr3.3:83734041-83734060 | MS.gene013029:CDS | 45.0% |
| !! | AGAACTTCTTCCTCTAGGGT+CGG | + | chr3.3:83734377-83734396 | MS.gene013029:CDS | 45.0% |
| !! | ATCATCGCTGATGGTTCTGA+GGG | + | chr3.3:83733896-83733915 | MS.gene013029:CDS | 45.0% |
| AACTATCGCCTTTGTTGGGC+AGG | + | chr3.3:83735052-83735071 | MS.gene013029:CDS | 50.0% | |
| ACCGGAACTATCGCCTTTGT+TGG | + | chr3.3:83735047-83735066 | MS.gene013029:CDS | 50.0% | |
| ATTGAGCACCTGCCCAACAA+AGG | - | chr3.3:83735063-83735082 | None:intergenic | 50.0% | |
| CCATCGACAAAAGAGAGACG+AGG | - | chr3.3:83734037-83734056 | None:intergenic | 50.0% | |
| CCCAACAAAGGCGATAGTTC+CGG | - | chr3.3:83735051-83735070 | None:intergenic | 50.0% | |
| CCGGAACTATCGCCTTTGTT+GGG | + | chr3.3:83735048-83735067 | MS.gene013029:CDS | 50.0% | |
| CGATGATGCTGAGCACAACT+TGG | - | chr3.3:83733883-83733902 | None:intergenic | 50.0% | |
| GCCAACTTTGCTTGAGAAGC+TGG | + | chr3.3:83734665-83734684 | MS.gene013029:CDS | 50.0% | |
| GCCAGCTTCTCAAGCAAAGT+TGG | - | chr3.3:83734669-83734688 | None:intergenic | 50.0% | |
| GTCAACCCTCCATTCGTGAA+AGG | - | chr3.3:83734825-83734844 | None:intergenic | 50.0% | |
| GTTGGAATCGGAGGAAGTCA+AGG | - | chr3.3:83733760-83733779 | None:intergenic | 50.0% | |
| TGGTGAGGGAAACGTTGCTT+TGG | - | chr3.3:83733793-83733812 | None:intergenic | 50.0% | |
| ! | ACGTTGCTTTGGTTGTTGGC+GGG | - | chr3.3:83733782-83733801 | None:intergenic | 50.0% |
| ! | AGTCAAGGGCGTATTCGGAT+CGG | - | chr3.3:83733745-83733764 | None:intergenic | 50.0% |
| ! | CATCATCGCTGATGGTTCTG+AGG | + | chr3.3:83733895-83733914 | MS.gene013029:CDS | 50.0% |
| ! | CCTCGTCTCTCTTTTGTCGA+TGG | + | chr3.3:83734034-83734053 | MS.gene013029:CDS | 50.0% |
| ! | GAGGAAGTCAAGGGCGTATT+CGG | - | chr3.3:83733750-83733769 | None:intergenic | 50.0% |
| ! | GGCAGCCCATCTTTTGCATT+TGG | - | chr3.3:83734648-83734667 | None:intergenic | 50.0% |
| ! | TTTTGTCGATGGTGTGTGGG+TGG | + | chr3.3:83734045-83734064 | MS.gene013029:CDS | 50.0% |
| AAGTTGTCGACCGACCCTAG+AGG | - | chr3.3:83734390-83734409 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | + | chr3.3:83735071-83735090 | MS.gene013029:CDS | 55.0% | |
| GTGCTCAGCATCATCGCTGA+TGG | + | chr3.3:83733887-83733906 | MS.gene013029:CDS | 55.0% | |
| ! | AAGGGCGTATTCGGATCGGA+GGG | - | chr3.3:83733741-83733760 | None:intergenic | 55.0% |
| ! | TGCTTTGGTTGTTGGCGGGT+TGG | - | chr3.3:83733778-83733797 | None:intergenic | 55.0% |
| !! | GGGAGCACCGTCTTTGAGAA+TGG | - | chr3.3:83734005-83734024 | None:intergenic | 55.0% |
| !! | GGTTGTTGGCGGGTTGGAAT+CGG | - | chr3.3:83733772-83733791 | None:intergenic | 55.0% |
| ! | CAAGGGCGTATTCGGATCGG+AGG | - | chr3.3:83733742-83733761 | None:intergenic | 60.0% |
| !! | TGTTGGCGGGTTGGAATCGG+AGG | - | chr3.3:83733769-83733788 | None:intergenic | 60.0% |
| AAAGACGGTGCTCCCGCAGG+TGG | + | chr3.3:83734010-83734029 | MS.gene013029:CDS | 65.0% | |
| AAGACGGTGCTCCCGCAGGT+GGG | + | chr3.3:83734011-83734030 | MS.gene013029:CDS | 65.0% | |
| AGAGAGACGAGGCCCACCTG+CGG | - | chr3.3:83734026-83734045 | None:intergenic | 65.0% | |
| CTCAAAGACGGTGCTCCCGC+AGG | + | chr3.3:83734007-83734026 | MS.gene013029:CDS | 65.0% | |
| GACGGTGGCTGCAGCAGCTT+TGG | - | chr3.3:83734934-83734953 | None:intergenic | 65.0% | |
| GAGAGACGAGGCCCACCTGC+GGG | - | chr3.3:83734025-83734044 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 83733592 | 83735097 | 83733592 | ID=MS.gene013029 |
| chr3.3 | mRNA | 83733592 | 83735097 | 83733592 | ID=MS.gene013029.t1;Parent=MS.gene013029 |
| chr3.3 | exon | 83733592 | 83733630 | 83733592 | ID=MS.gene013029.t1.exon1;Parent=MS.gene013029.t1 |
| chr3.3 | CDS | 83733592 | 83733630 | 83733592 | ID=cds.MS.gene013029.t1;Parent=MS.gene013029.t1 |
| chr3.3 | exon | 83733701 | 83734156 | 83733701 | ID=MS.gene013029.t1.exon2;Parent=MS.gene013029.t1 |
| chr3.3 | CDS | 83733701 | 83734156 | 83733701 | ID=cds.MS.gene013029.t1;Parent=MS.gene013029.t1 |
| chr3.3 | exon | 83734315 | 83735097 | 83734315 | ID=MS.gene013029.t1.exon3;Parent=MS.gene013029.t1 |
| chr3.3 | CDS | 83734315 | 83735097 | 83734315 | ID=cds.MS.gene013029.t1;Parent=MS.gene013029.t1 |
| Gene Sequence |
| Protein sequence |
