Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013093.t1 | XP_003602889.2 | 99.4 | 2341 | 14 | 0 | 16 | 2356 | 914 | 3254 | 0.00E+00 | 4638.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013093.t1 | F4I9T0 | 66.9 | 2346 | 748 | 17 | 18 | 2354 | 278 | 2604 | 0.0e+00 | 3132.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013093.t1 | G7J4K4 | 99.4 | 2341 | 14 | 0 | 16 | 2356 | 914 | 3254 | 0.0e+00 | 4638.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013093 | MS.gene029229 | PPI |
| MS.gene013093 | MS.gene071666 | PPI |
| MS.gene013093 | MS.gene037346 | PPI |
| MS.gene071668 | MS.gene013093 | PPI |
| MS.gene013093 | MS.gene44408 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013093.t1 | MTR_3g100060 | 99.402 | 2341 | 14 | 0 | 16 | 2356 | 914 | 3254 | 0.0 | 4825 |
| MS.gene013093.t1 | MTR_8g035750 | 31.996 | 997 | 537 | 26 | 1326 | 2266 | 1884 | 2795 | 1.59e-126 | 448 |
| MS.gene013093.t1 | MTR_8g035750 | 23.182 | 729 | 438 | 23 | 98 | 738 | 269 | 963 | 3.16e-29 | 129 |
| MS.gene013093.t1 | MTR_7g075660 | 32.427 | 993 | 529 | 30 | 1333 | 2266 | 1898 | 2807 | 1.45e-122 | 435 |
| MS.gene013093.t1 | MTR_7g075660 | 22.085 | 729 | 446 | 19 | 98 | 738 | 283 | 977 | 3.14e-29 | 129 |
| MS.gene013093.t1 | MTR_1g071260 | 40.901 | 555 | 289 | 11 | 1612 | 2150 | 2908 | 3439 | 7.81e-109 | 391 |
| MS.gene013093.t1 | MTR_1g102710 | 32.517 | 286 | 143 | 14 | 1702 | 1985 | 328 | 565 | 1.16e-24 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013093.t1 | AT1G58230 | 66.965 | 2346 | 747 | 17 | 18 | 2354 | 278 | 2604 | 0.0 | 3218 |
| MS.gene013093.t1 | AT1G58230 | 66.965 | 2346 | 747 | 17 | 18 | 2354 | 72 | 2398 | 0.0 | 3217 |
| MS.gene013093.t1 | AT1G58230 | 66.922 | 2346 | 748 | 17 | 18 | 2354 | 278 | 2604 | 0.0 | 3216 |
| MS.gene013093.t1 | AT2G45540 | 31.837 | 980 | 517 | 24 | 1353 | 2266 | 1933 | 2827 | 1.62e-122 | 435 |
| MS.gene013093.t1 | AT2G45540 | 23.553 | 743 | 456 | 22 | 98 | 758 | 269 | 981 | 4.16e-31 | 135 |
| MS.gene013093.t1 | AT2G45540 | 31.837 | 980 | 517 | 24 | 1353 | 2266 | 1933 | 2827 | 1.62e-122 | 435 |
| MS.gene013093.t1 | AT2G45540 | 23.553 | 743 | 456 | 22 | 98 | 758 | 269 | 981 | 4.16e-31 | 135 |
| MS.gene013093.t1 | AT2G45540 | 31.837 | 980 | 517 | 24 | 1353 | 2266 | 1933 | 2827 | 1.62e-122 | 435 |
| MS.gene013093.t1 | AT2G45540 | 23.553 | 743 | 456 | 22 | 98 | 758 | 269 | 981 | 4.16e-31 | 135 |
| MS.gene013093.t1 | AT2G45540 | 31.837 | 980 | 517 | 24 | 1353 | 2266 | 1933 | 2827 | 1.62e-122 | 435 |
| MS.gene013093.t1 | AT2G45540 | 23.553 | 743 | 456 | 22 | 98 | 758 | 269 | 981 | 4.16e-31 | 135 |
| MS.gene013093.t1 | AT2G45540 | 31.837 | 980 | 517 | 24 | 1353 | 2266 | 1933 | 2827 | 1.62e-122 | 435 |
| MS.gene013093.t1 | AT2G45540 | 23.553 | 743 | 456 | 22 | 98 | 758 | 269 | 981 | 4.16e-31 | 135 |
| MS.gene013093.t1 | AT2G45540 | 31.837 | 980 | 517 | 24 | 1353 | 2266 | 1988 | 2882 | 1.97e-122 | 435 |
| MS.gene013093.t1 | AT2G45540 | 23.810 | 567 | 338 | 16 | 98 | 590 | 269 | 815 | 7.32e-27 | 121 |
| MS.gene013093.t1 | AT4G02660 | 37.941 | 680 | 312 | 20 | 1614 | 2276 | 2870 | 3456 | 1.32e-112 | 403 |
| MS.gene013093.t1 | AT4G02660 | 37.941 | 680 | 312 | 20 | 1614 | 2276 | 2821 | 3407 | 1.50e-112 | 403 |
| MS.gene013093.t1 | AT4G02660 | 37.941 | 680 | 312 | 20 | 1614 | 2276 | 2821 | 3407 | 1.50e-112 | 403 |
| MS.gene013093.t1 | AT1G03060 | 41.652 | 557 | 282 | 13 | 1612 | 2150 | 2878 | 3409 | 9.34e-110 | 394 |
| MS.gene013093.t1 | AT1G03060 | 41.652 | 557 | 282 | 13 | 1612 | 2150 | 2849 | 3380 | 9.43e-110 | 394 |
| MS.gene013093.t1 | AT1G03060 | 41.652 | 557 | 282 | 13 | 1612 | 2150 | 2848 | 3379 | 9.51e-110 | 394 |
| MS.gene013093.t1 | AT1G03060 | 41.652 | 557 | 282 | 13 | 1612 | 2150 | 2877 | 3408 | 1.06e-109 | 394 |
| MS.gene013093.t1 | AT3G60920 | 46.091 | 243 | 119 | 5 | 1606 | 1847 | 1597 | 1828 | 1.23e-54 | 212 |
| MS.gene013093.t1 | AT3G60920 | 22.317 | 587 | 365 | 17 | 184 | 708 | 7 | 564 | 1.08e-20 | 101 |
| MS.gene013093.t1 | AT3G60920 | 46.091 | 243 | 119 | 5 | 1606 | 1847 | 1648 | 1879 | 1.47e-54 | 212 |
| MS.gene013093.t1 | AT3G60920 | 22.411 | 647 | 402 | 20 | 127 | 708 | 4 | 615 | 6.21e-23 | 108 |
| MS.gene013093.t1 | AT5G18525 | 32.271 | 251 | 139 | 12 | 1685 | 1932 | 333 | 555 | 8.07e-25 | 114 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 21210 | gene | 335 | 17252 | 335 | ID=MS.gene013093 |
| 21210 | mRNA | 335 | 17252 | 335 | ID=MS.gene013093.t1;Parent=MS.gene013093 |
| 21210 | exon | 335 | 538 | 335 | ID=MS.gene013093.t1.exon1;Parent=MS.gene013093.t1 |
| 21210 | CDS | 335 | 538 | 335 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 1766 | 1807 | 1766 | ID=MS.gene013093.t1.exon2;Parent=MS.gene013093.t1 |
| 21210 | CDS | 1766 | 1807 | 1766 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 1929 | 2273 | 1929 | ID=MS.gene013093.t1.exon3;Parent=MS.gene013093.t1 |
| 21210 | CDS | 1929 | 2273 | 1929 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 3287 | 3460 | 3287 | ID=MS.gene013093.t1.exon4;Parent=MS.gene013093.t1 |
| 21210 | CDS | 3287 | 3460 | 3287 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 4238 | 4398 | 4238 | ID=MS.gene013093.t1.exon5;Parent=MS.gene013093.t1 |
| 21210 | CDS | 4238 | 4398 | 4238 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 4502 | 4820 | 4502 | ID=MS.gene013093.t1.exon6;Parent=MS.gene013093.t1 |
| 21210 | CDS | 4502 | 4820 | 4502 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 4969 | 5398 | 4969 | ID=MS.gene013093.t1.exon7;Parent=MS.gene013093.t1 |
| 21210 | CDS | 4969 | 5398 | 4969 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 5500 | 5836 | 5500 | ID=MS.gene013093.t1.exon8;Parent=MS.gene013093.t1 |
| 21210 | CDS | 5500 | 5836 | 5500 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 5961 | 6149 | 5961 | ID=MS.gene013093.t1.exon9;Parent=MS.gene013093.t1 |
| 21210 | CDS | 5961 | 6149 | 5961 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 6395 | 6650 | 6395 | ID=MS.gene013093.t1.exon10;Parent=MS.gene013093.t1 |
| 21210 | CDS | 6395 | 6650 | 6395 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 6842 | 7033 | 6842 | ID=MS.gene013093.t1.exon11;Parent=MS.gene013093.t1 |
| 21210 | CDS | 6842 | 7033 | 6842 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 7114 | 7332 | 7114 | ID=MS.gene013093.t1.exon12;Parent=MS.gene013093.t1 |
| 21210 | CDS | 7114 | 7332 | 7114 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 7480 | 7656 | 7480 | ID=MS.gene013093.t1.exon13;Parent=MS.gene013093.t1 |
| 21210 | CDS | 7480 | 7656 | 7480 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 8410 | 8522 | 8410 | ID=MS.gene013093.t1.exon14;Parent=MS.gene013093.t1 |
| 21210 | CDS | 8410 | 8522 | 8410 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 8637 | 9101 | 8637 | ID=MS.gene013093.t1.exon15;Parent=MS.gene013093.t1 |
| 21210 | CDS | 8637 | 9101 | 8637 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 9251 | 9464 | 9251 | ID=MS.gene013093.t1.exon16;Parent=MS.gene013093.t1 |
| 21210 | CDS | 9251 | 9464 | 9251 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 9943 | 10164 | 9943 | ID=MS.gene013093.t1.exon17;Parent=MS.gene013093.t1 |
| 21210 | CDS | 9943 | 10164 | 9943 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 10283 | 10753 | 10283 | ID=MS.gene013093.t1.exon18;Parent=MS.gene013093.t1 |
| 21210 | CDS | 10283 | 10753 | 10283 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 10847 | 11170 | 10847 | ID=MS.gene013093.t1.exon19;Parent=MS.gene013093.t1 |
| 21210 | CDS | 10847 | 11170 | 10847 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 11278 | 11730 | 11278 | ID=MS.gene013093.t1.exon20;Parent=MS.gene013093.t1 |
| 21210 | CDS | 11278 | 11730 | 11278 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 11822 | 11868 | 11822 | ID=MS.gene013093.t1.exon21;Parent=MS.gene013093.t1 |
| 21210 | CDS | 11822 | 11868 | 11822 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 12256 | 12349 | 12256 | ID=MS.gene013093.t1.exon22;Parent=MS.gene013093.t1 |
| 21210 | CDS | 12256 | 12349 | 12256 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 14584 | 14790 | 14584 | ID=MS.gene013093.t1.exon23;Parent=MS.gene013093.t1 |
| 21210 | CDS | 14584 | 14790 | 14584 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 15207 | 15332 | 15207 | ID=MS.gene013093.t1.exon24;Parent=MS.gene013093.t1 |
| 21210 | CDS | 15207 | 15332 | 15207 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 15441 | 15812 | 15441 | ID=MS.gene013093.t1.exon25;Parent=MS.gene013093.t1 |
| 21210 | CDS | 15441 | 15812 | 15441 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 15930 | 16161 | 15930 | ID=MS.gene013093.t1.exon26;Parent=MS.gene013093.t1 |
| 21210 | CDS | 15930 | 16161 | 15930 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| 21210 | exon | 16567 | 17252 | 16567 | ID=MS.gene013093.t1.exon27;Parent=MS.gene013093.t1 |
| 21210 | CDS | 16567 | 17252 | 16567 | ID=cds.MS.gene013093.t1;Parent=MS.gene013093.t1 |
| Gene Sequence |
| Protein sequence |
