Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013168.t1 | XP_003602781.1 | 98.4 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 4.80E-214 | 753.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013168.t1 | Q0E0Y3 | 69.0 | 377 | 106 | 3 | 3 | 369 | 8 | 383 | 8.5e-151 | 534.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013168.t1 | G7J3D1 | 98.4 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 3.5e-214 | 753.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013168 | MS.gene005659 | PPI |
| MS.gene013168 | MS.gene07153 | PPI |
| MS.gene02998 | MS.gene013168 | PPI |
| MS.gene013168 | MS.gene21545 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013168.t1 | MTR_3g098940 | 98.374 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 0.0 | 756 |
| MS.gene013168.t1 | MTR_3g098940 | 98.225 | 338 | 6 | 0 | 1 | 338 | 12 | 349 | 0.0 | 686 |
| MS.gene013168.t1 | MTR_1g095580 | 26.616 | 263 | 163 | 7 | 42 | 275 | 29 | 290 | 8.12e-17 | 80.9 |
| MS.gene013168.t1 | MTR_1g095580 | 26.616 | 263 | 163 | 7 | 42 | 275 | 29 | 290 | 1.43e-16 | 80.1 |
| MS.gene013168.t1 | MTR_1g095580 | 25.490 | 306 | 185 | 9 | 42 | 313 | 29 | 325 | 3.11e-16 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013168.t1 | AT5G51140 | 68.232 | 362 | 95 | 4 | 16 | 369 | 46 | 395 | 6.51e-178 | 500 |
| MS.gene013168.t1 | AT5G51140 | 67.989 | 353 | 93 | 4 | 16 | 360 | 46 | 386 | 6.83e-171 | 483 |
| MS.gene013168.t1 | AT3G52260 | 25.667 | 300 | 191 | 8 | 45 | 313 | 34 | 332 | 1.25e-17 | 84.0 |
| MS.gene013168.t1 | AT3G52260 | 26.894 | 264 | 162 | 7 | 45 | 277 | 34 | 297 | 2.80e-17 | 82.4 |
| MS.gene013168.t1 | AT3G52260 | 26.357 | 258 | 159 | 7 | 45 | 271 | 34 | 291 | 4.97e-15 | 75.1 |
| MS.gene013168.t1 | AT3G52260 | 26.772 | 254 | 149 | 7 | 71 | 294 | 91 | 337 | 1.50e-12 | 68.9 |
Find 88 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCCCTTCGGTGCCAAATT+TGG | 0.184524 | 3.3:+81360297 | None:intergenic |
| CGCGTTCATTTACAGTCTTC+TGG | 0.226994 | 3.3:-81360513 | MS.gene013168:CDS |
| TAGCATTTCTGGCTATAATA+AGG | 0.255944 | 3.3:+81365114 | None:intergenic |
| GTGTACCAACTGTCCAAATT+TGG | 0.306598 | 3.3:-81360310 | MS.gene013168:CDS |
| AGTTTCGCCCAATCAGGATA+TGG | 0.308758 | 3.3:+81359586 | None:intergenic |
| TTGTTTGCCGAGGAATTTAA+AGG | 0.318138 | 3.3:-81367267 | MS.gene013168:CDS |
| TCGAGCATTATAGTCTATAT+TGG | 0.345385 | 3.3:+81362414 | None:intergenic |
| GCTTCTTTGACATCAAATTT+CGG | 0.348641 | 3.3:-81360393 | MS.gene013168:CDS |
| TGCGGTAAAGGAGGATTTGC+TGG | 0.374155 | 3.3:+81367579 | None:intergenic |
| AATATATTGCAAAAGTAGTT+GGG | 0.377867 | 3.3:-81363289 | MS.gene013168:CDS |
| TACAGCATGTCATTGGCTAT+TGG | 0.388217 | 3.3:+81360486 | None:intergenic |
| GAGTACCATTGCTACTAATC+CGG | 0.396097 | 3.3:+81361121 | None:intergenic |
| CAATATATTGCAAAAGTAGT+TGG | 0.398952 | 3.3:-81363290 | MS.gene013168:CDS |
| CCAGCCCATGTTCTGCTTGA+AGG | 0.399831 | 3.3:+81365455 | None:intergenic |
| ACTGTTGATAGATCTATTAA+TGG | 0.400312 | 3.3:-81360450 | MS.gene013168:CDS |
| ATGAATGCCCATATCCTGAT+TGG | 0.401822 | 3.3:-81359594 | MS.gene013168:CDS |
| ATCAATGCTAAACTCCTCAT+TGG | 0.413066 | 3.3:+81360338 | None:intergenic |
| TTACAAACAGTTAGCACATC+TGG | 0.415839 | 3.3:+81366844 | None:intergenic |
| AGCTAAAGTTTCGCCCAATC+AGG | 0.416919 | 3.3:+81359580 | None:intergenic |
| ACGATGTAATCTTGCGGTAA+AGG | 0.424137 | 3.3:+81367567 | None:intergenic |
| TCTGGTTCTGTATGAAGAAT+AGG | 0.424198 | 3.3:+81366862 | None:intergenic |
| ACAGCATGTCATTGGCTATT+GGG | 0.433925 | 3.3:+81360487 | None:intergenic |
| TATCGCAAGAACACTGTTGT+TGG | 0.444093 | 3.3:-81365481 | MS.gene013168:CDS |
| AAGGTCAGAGACTCTACAAA+AGG | 0.453239 | 3.3:-81361168 | MS.gene013168:CDS |
| CGGTGCCAAATTTGGACAGT+TGG | 0.457022 | 3.3:+81360305 | None:intergenic |
| AGTCACTGGCCGCACTCATC+AGG | 0.471463 | 3.3:-81361076 | MS.gene013168:intron |
| GATTTAACTACGTATGAAAC+CGG | 0.475395 | 3.3:+81367019 | None:intergenic |
| GATTCAAGTTGACGGTGAAA+TGG | 0.476238 | 3.3:-81367044 | MS.gene013168:CDS |
| CTGTCCAAATTTGGCACCGA+AGG | 0.478848 | 3.3:-81360301 | MS.gene013168:CDS |
| CTGTTGATAGATCTATTAAT+GGG | 0.482702 | 3.3:-81360449 | MS.gene013168:CDS |
| CCTTCAAGCAGAACATGGGC+TGG | 0.487356 | 3.3:-81365455 | MS.gene013168:CDS |
| AAACTTACATAATATTCATA+AGG | 0.492762 | 3.3:+81367240 | None:intergenic |
| CGACTGGATCGCCTTGTCTC+CGG | 0.493010 | 3.3:-81365140 | MS.gene013168:CDS |
| TGATGAGTGCGGCCAGTGAC+TGG | 0.494032 | 3.3:+81361078 | None:intergenic |
| TGAGATGTACAGCATGTCAT+TGG | 0.495292 | 3.3:+81360479 | None:intergenic |
| TGGTTGTTACAGAGTGAGGC+TGG | 0.502839 | 3.3:-81363326 | MS.gene013168:intron |
| TAAAGAATTGCATACCCCTT+CGG | 0.504219 | 3.3:+81360285 | None:intergenic |
| GCGATGAATTCGAAGTAGTA+TGG | 0.504359 | 3.3:+81367430 | None:intergenic |
| GTTTCGCCCAATCAGGATAT+GGG | 0.504686 | 3.3:+81359587 | None:intergenic |
| TGAATGCCCATATCCTGATT+GGG | 0.506186 | 3.3:-81359593 | MS.gene013168:CDS |
| TTACATTGTATGCGGTACTC+TGG | 0.510906 | 3.3:-81359631 | MS.gene013168:CDS |
| CAAATAAAATACCTCATCCT+CGG | 0.512397 | 3.3:+81363259 | None:intergenic |
| AAATAAAATACCTCATCCTC+GGG | 0.514851 | 3.3:+81363260 | None:intergenic |
| GGCATCCTTCAAGCAGAACA+TGG | 0.516228 | 3.3:-81365460 | MS.gene013168:CDS |
| TGTCTCAGCTGTCCATCGAC+TGG | 0.517338 | 3.3:-81365156 | MS.gene013168:intron |
| TGGGGAATTTCCCGAGGATG+AGG | 0.518507 | 3.3:-81363270 | MS.gene013168:intron |
| CAGGCATGAACCACCTGTGA+TGG | 0.518717 | 3.3:-81366896 | MS.gene013168:CDS |
| TCTGGCTATAATAAGGAGTC+CGG | 0.521448 | 3.3:+81365121 | None:intergenic |
| AGTACCATTGCTACTAATCC+GGG | 0.522821 | 3.3:+81361122 | None:intergenic |
| AGGTCGGCCTTTAAATTCCT+CGG | 0.523733 | 3.3:+81367260 | None:intergenic |
| AGATATGATACTGATGAAGA+AGG | 0.536074 | 3.3:-81359661 | MS.gene013168:CDS |
| GGGCATTCATATGTCCATCC+AGG | 0.555137 | 3.3:+81359607 | None:intergenic |
| AGTTGACGGTGAAATGGTGC+CGG | 0.558967 | 3.3:-81367038 | MS.gene013168:CDS |
| TAGGTTAGTGCGGTGAAATG+TGG | 0.563936 | 3.3:-81367070 | MS.gene013168:CDS |
| TGTCCAAATTTGGCACCGAA+GGG | 0.570374 | 3.3:-81360300 | MS.gene013168:CDS |
| AGGTCAGAGACTCTACAAAA+GGG | 0.571744 | 3.3:-81361167 | MS.gene013168:CDS |
| TGTGGAAGGATTCAAGTTGA+CGG | 0.572120 | 3.3:-81367052 | MS.gene013168:CDS |
| CAAGATTACATCGTTCGAAA+CGG | 0.576471 | 3.3:-81367558 | MS.gene013168:intron |
| TTGCCTCCACAAAATTTACC+CGG | 0.581737 | 3.3:-81361140 | MS.gene013168:CDS |
| TGCGGTACTCTGGACCTGGA+TGG | 0.588015 | 3.3:-81359621 | MS.gene013168:CDS |
| ATACTGATGAAGAAGGTTTG+TGG | 0.590363 | 3.3:-81359654 | MS.gene013168:CDS |
| AGACAAGGCGATCCAGTCGA+TGG | 0.592536 | 3.3:+81365144 | None:intergenic |
| TTACATAATATTCATAAGGT+CGG | 0.595153 | 3.3:+81367244 | None:intergenic |
| TATTGTAGATTTGTTTGCCG+AGG | 0.596682 | 3.3:-81367277 | MS.gene013168:CDS |
| ATAGACTATAATGCTCGAGA+AGG | 0.597554 | 3.3:-81362409 | MS.gene013168:CDS |
| TGTATGCGGTACTCTGGACC+TGG | 0.597884 | 3.3:-81359625 | MS.gene013168:CDS |
| TAATAAGGAGTCCGGAGACA+AGG | 0.599949 | 3.3:+81365129 | None:intergenic |
| TTTACCCGGATTAGTAGCAA+TGG | 0.600058 | 3.3:-81361126 | MS.gene013168:CDS |
| GGAACATCACAAGCCATCAC+AGG | 0.601035 | 3.3:+81366883 | None:intergenic |
| AGAAGGAAGAAGCACAGCAG+AGG | 0.605852 | 3.3:-81362392 | MS.gene013168:intron |
| ATATATTGCAAAAGTAGTTG+GGG | 0.609841 | 3.3:-81363288 | MS.gene013168:CDS |
| TTACTTACAGGGACAGACGC+AGG | 0.614034 | 3.3:+81366820 | None:intergenic |
| TTAGTGCGGTGAAATGTGGA+AGG | 0.615192 | 3.3:-81367066 | MS.gene013168:CDS |
| AGCCAGAAATGCTACAAAAG+CGG | 0.615290 | 3.3:-81365105 | MS.gene013168:CDS |
| ATGTAATCTTGCGGTAAAGG+AGG | 0.615710 | 3.3:+81367570 | None:intergenic |
| TTTGGACAGTTGGTACACAT+AGG | 0.616779 | 3.3:+81360315 | None:intergenic |
| CAGAGACTCTACAAAAGGGA+AGG | 0.630227 | 3.3:-81361163 | MS.gene013168:CDS |
| GTATGGATCTTAGGTTAGTG+CGG | 0.630653 | 3.3:-81367080 | MS.gene013168:intron |
| GTTTGTGGTTACATTGTATG+CGG | 0.636819 | 3.3:-81359639 | MS.gene013168:CDS |
| GTCCAAATTTGGCACCGAAG+GGG | 0.645456 | 3.3:-81360299 | MS.gene013168:intron |
| GCATCCTTCAAGCAGAACAT+GGG | 0.646483 | 3.3:-81365459 | MS.gene013168:CDS |
| TTCTTGCGATATTGACCACA+TGG | 0.665528 | 3.3:+81365493 | None:intergenic |
| TTGTCAAGAAAATTCCAATG+AGG | 0.670627 | 3.3:-81360352 | MS.gene013168:CDS |
| ACATCACAAGCCATCACAGG+TGG | 0.674685 | 3.3:+81366886 | None:intergenic |
| AGTAGTTGGGGAATTTCCCG+AGG | 0.679238 | 3.3:-81363276 | MS.gene013168:CDS |
| TTTCGAACGATGTAATCTTG+CGG | 0.684194 | 3.3:+81367561 | None:intergenic |
| AAGAGTGTATGGATATCGTG+TGG | 0.692668 | 3.3:-81367608 | None:intergenic |
| GGTACATAGGTTCATCCATG+TGG | 0.702272 | 3.3:-81365508 | MS.gene013168:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 81359582 | 81367622 | 81359582 | ID=MS.gene013168 |
| chr3.3 | mRNA | 81359582 | 81367622 | 81359582 | ID=MS.gene013168.t1;Parent=MS.gene013168 |
| chr3.3 | exon | 81367559 | 81367622 | 81367559 | ID=MS.gene013168.t1.exon1;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81367559 | 81367622 | 81367559 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81367426 | 81367469 | 81367426 | ID=MS.gene013168.t1.exon2;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81367426 | 81367469 | 81367426 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81367248 | 81367325 | 81367248 | ID=MS.gene013168.t1.exon3;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81367248 | 81367325 | 81367248 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81366989 | 81367089 | 81366989 | ID=MS.gene013168.t1.exon4;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81366989 | 81367089 | 81366989 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81366828 | 81366915 | 81366828 | ID=MS.gene013168.t1.exon5;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81366828 | 81366915 | 81366828 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81365443 | 81365521 | 81365443 | ID=MS.gene013168.t1.exon6;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81365443 | 81365521 | 81365443 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81365088 | 81365170 | 81365088 | ID=MS.gene013168.t1.exon7;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81365088 | 81365170 | 81365088 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81363271 | 81363336 | 81363271 | ID=MS.gene013168.t1.exon8;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81363271 | 81363336 | 81363271 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81362393 | 81362449 | 81362393 | ID=MS.gene013168.t1.exon9;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81362393 | 81362449 | 81362393 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81361077 | 81361187 | 81361077 | ID=MS.gene013168.t1.exon10;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81361077 | 81361187 | 81361077 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81360300 | 81360538 | 81360300 | ID=MS.gene013168.t1.exon11;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81360300 | 81360538 | 81360300 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| chr3.3 | exon | 81359582 | 81359681 | 81359582 | ID=MS.gene013168.t1.exon12;Parent=MS.gene013168.t1 |
| chr3.3 | CDS | 81359582 | 81359681 | 81359582 | ID=cds.MS.gene013168.t1;Parent=MS.gene013168.t1 |
| Gene Sequence |
| Protein sequence |
