AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015095

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015095.t1 MTR_3g102920 83.032 442 41 2 1 441 1 409 0.0 723
MS.gene015095.t1 MTR_4g045707 66.402 378 119 4 66 440 14 386 2.97e-175 496
MS.gene015095.t1 MTR_3g101010 68.338 379 112 4 66 440 13 387 1.49e-172 489
MS.gene015095.t1 MTR_3g048000 61.779 416 125 7 31 442 7 392 1.02e-169 482
MS.gene015095.t1 MTR_3g018740 64.286 378 128 4 66 440 13 386 2.23e-167 476
MS.gene015095.t1 MTR_8g027395 61.386 404 144 6 41 441 2 396 1.08e-163 467
MS.gene015095.t1 MTR_8g461160 62.051 390 134 4 62 442 10 394 1.52e-161 461
MS.gene015095.t1 MTR_3g101030 58.950 419 144 6 46 440 13 427 2.57e-161 462
MS.gene015095.t1 MTR_7g451890 57.214 402 156 4 53 440 1 400 2.72e-161 461
MS.gene015095.t1 MTR_3g101130 59.740 385 144 3 62 441 10 388 2.52e-158 453
MS.gene015095.t1 MTR_7g047780 57.214 402 156 4 53 440 1 400 9.53e-158 452
MS.gene015095.t1 MTR_7g050750 59.481 385 148 3 62 440 12 394 3.45e-157 450
MS.gene015095.t1 MTR_4g088525 62.810 363 127 3 82 441 11 368 3.54e-153 439
MS.gene015095.t1 MTR_3g101180 58.524 393 149 4 62 442 10 400 8.84e-153 439
MS.gene015095.t1 MTR_8g027420 60.526 380 129 5 65 441 13 374 1.95e-152 437
MS.gene015095.t1 MTR_7g050810 56.235 409 162 6 40 440 3 402 3.15e-152 438
MS.gene015095.t1 MTR_2g046030 59.791 383 139 6 65 440 12 386 2.64e-148 427
MS.gene015095.t1 MTR_2g045980 58.486 383 144 6 65 440 12 386 2.27e-144 417
MS.gene015095.t1 MTR_3g101050 58.854 384 129 6 66 442 37 398 1.30e-140 408
MS.gene015095.t1 MTR_4g088535 56.266 391 145 6 54 441 3 370 5.30e-140 406
MS.gene015095.t1 MTR_3g015620 56.771 384 130 5 65 440 23 378 1.62e-136 397
MS.gene015095.t1 MTR_3g101200 52.882 399 147 8 56 442 23 392 2.81e-120 356
MS.gene015095.t1 MTR_3g100995 66.016 256 80 2 188 440 11 262 4.59e-116 340
MS.gene015095.t1 MTR_3g101190 49.036 363 118 4 82 441 13 311 4.92e-110 327
MS.gene015095.t1 MTR_7g050830 50.303 330 128 4 46 352 3 319 6.19e-109 325
MS.gene015095.t1 MTR_3g018747 63.740 262 73 4 184 440 9 253 2.11e-107 318
MS.gene015095.t1 MTR_3g100010 54.045 309 94 2 80 355 37 330 2.53e-107 321
MS.gene015095.t1 MTR_3g015760 55.714 280 108 4 169 440 8 279 2.56e-98 296
MS.gene015095.t1 MTR_3g111160 63.636 220 74 4 225 440 1 218 1.34e-82 253
MS.gene015095.t1 MTR_3g114310 42.627 373 196 11 82 439 38 407 9.11e-80 253
MS.gene015095.t1 MTR_3g100520 58.371 221 75 4 225 441 1 208 2.80e-78 242
MS.gene015095.t1 MTR_3g099970 64.141 198 49 4 184 378 20 198 5.75e-76 236
MS.gene015095.t1 MTR_2g046410 61.836 207 69 4 241 440 144 347 8.13e-76 241
MS.gene015095.t1 MTR_3g101110 53.750 240 79 3 187 420 71 284 2.24e-73 232
MS.gene015095.t1 MTR_3g101020 56.831 183 54 1 258 440 1 158 3.84e-60 194
MS.gene015095.t1 MTR_3g102870 59.494 158 59 2 247 401 1 156 4.01e-53 176
MS.gene015095.t1 MTR_3g101150 60.625 160 53 4 290 441 1 158 8.96e-53 174
MS.gene015095.t1 MTR_3g111150 48.851 174 63 3 65 238 6 153 4.31e-50 167
MS.gene015095.t1 MTR_3g100990 57.664 137 49 2 49 185 3 130 4.84e-41 153
MS.gene015095.t1 MTR_4g093550 53.247 77 34 1 67 143 15 89 4.94e-19 82.0
MS.gene015095.t1 MTR_3g101140 45.000 120 50 2 323 440 9 114 1.16e-17 79.0
MS.gene015095.t1 MTR_6g046890 53.247 77 33 1 75 151 14 87 2.86e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015095.t1 AT1G47710 48.819 381 188 4 65 440 12 390 1.53e-127 375
MS.gene015095.t1 AT1G47710 46.429 420 200 6 26 440 18 417 1.93e-127 375
MS.gene015095.t1 AT2G25240 46.891 386 187 8 60 439 9 382 1.49e-110 331
MS.gene015095.t1 AT3G45220 46.448 366 181 6 84 439 30 390 2.33e-107 323
MS.gene015095.t1 AT2G26390 44.125 383 196 6 66 439 13 386 4.00e-102 310
MS.gene015095.t1 AT1G62170 40.274 365 191 3 81 439 88 431 4.28e-91 283
MS.gene015095.t1 AT2G14540 43.717 382 184 6 65 439 40 397 1.50e-90 281
MS.gene015095.t1 AT1G64030 43.158 380 189 7 66 439 13 371 2.81e-90 279
MS.gene015095.t1 AT1G62170 36.935 398 191 4 81 439 88 464 4.72e-84 266
MS.gene015095.t1 AT2G35580 41.011 356 184 6 91 439 35 371 1.29e-81 256
MS.gene015095.t1 AT1G64010 44.907 216 97 4 225 439 1 195 9.01e-54 178
MS.gene015095.t1 AT1G64010 44.776 201 89 4 240 439 2 181 3.13e-47 161
MS.gene015095.t1 AT1G63280 52.174 115 51 2 184 296 8 120 7.91e-31 115
MS.gene015095.t1 AT1G62160 41.758 182 83 2 260 439 58 218 3.40e-29 114
MS.gene015095.t1 AT1G51330 47.748 111 55 2 183 290 30 140 3.67e-25 102
MS.gene015095.t1 AT1G64020 36.036 111 68 3 65 174 12 120 1.55e-13 67.4

Find 111 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTTCTCTTTGTCTAAAA+TGG 0.207924 3.2:-85347426 MS.gene015095:CDS
AATGGAGAGAAGCAGTTTAT+TGG 0.238977 3.2:-85347707 MS.gene015095:CDS
TGAAAGCCTTGTGAAATATC+TGG 0.242102 3.2:+85347363 None:intergenic
AATCGTCTCTACTGATTATA+AGG 0.274934 3.2:-85348281 MS.gene015095:CDS
GTATCTTCTCCTTTGTTATA+AGG 0.293458 3.2:+85347653 None:intergenic
AAGGCCATTTGTCTCCGTTT+TGG 0.321136 3.2:+85347898 None:intergenic
TCAAATGAAACCTTGAATCT+TGG 0.321190 3.2:+85347491 None:intergenic
GACAGTCCATCTCTTGCATT+TGG 0.337846 3.2:+85347602 None:intergenic
AAGAGATGGACTGTCTTCTT+TGG 0.343723 3.2:-85347594 MS.gene015095:CDS
TCTGATATGCTGAAGGAGTT+AGG 0.356782 3.2:-85347458 MS.gene015095:CDS
TGAGCACATGTCCAACAAAA+AGG 0.363030 3.2:+85347195 None:intergenic
GAAATATCTGGGAAACAAAA+TGG 0.364280 3.2:+85347375 None:intergenic
TGGCGGCTCCGGTGAGGGCG+AGG 0.367351 3.2:+85348757 None:intergenic
TGAAGGAAGGTTGAAGAGAA+AGG 0.399705 3.2:+85348308 None:intergenic
CTAATGCATTGTACTTCAAA+GGG 0.400535 3.2:-85347820 MS.gene015095:CDS
GAATCAACAATTTGATGCTT+CGG 0.418605 3.2:-85347792 MS.gene015095:CDS
CTTCAGAATTCCAAGATTCA+AGG 0.421035 3.2:-85347501 MS.gene015095:CDS
AGTAGAGACGATTTGTTTGA+AGG 0.421065 3.2:+85348291 None:intergenic
GGCGGCTCCGGTGAGGGCGA+GGG 0.431624 3.2:+85348758 None:intergenic
CTCTATCCCCTCGCCCTCAC+CGG 0.434026 3.2:-85348765 MS.gene015095:CDS
CTCTCGTTCTCCTGTGCCAC+GGG 0.438774 3.2:-85348671 MS.gene015095:CDS
GCTAATGCATTGTACTTCAA+AGG 0.441327 3.2:-85347821 MS.gene015095:CDS
GTTCTTGTTTGGAGAGCAAA+TGG 0.441830 3.2:+85348563 None:intergenic
GAAGGAAGGTTGAAGAGAAA+GGG 0.442090 3.2:+85348309 None:intergenic
GTCTTCTTTGGTTGAGAAGG+TGG 0.446506 3.2:-85347582 MS.gene015095:CDS
CGTCTTTCTAAAGTCGAAGT+AGG 0.447049 3.2:-85347527 MS.gene015095:CDS
TTCATTGAAGTGAATGAAGA+AGG 0.449995 3.2:-85347344 MS.gene015095:CDS
CGGTTGTAAGAAGGAGGTGG+AGG 0.454172 3.2:+85348694 None:intergenic
GAGTGATGCTGTGGCGGCTC+CGG 0.454778 3.2:+85348746 None:intergenic
TGCGGCTGGTCCCGTGGCAC+AGG 0.459049 3.2:+85348661 None:intergenic
CAAGGCCCACCGGCAGGAGA+AGG 0.460774 3.2:+85348367 None:intergenic
CTTCTCTCCATTGGTCATGA+AGG 0.460859 3.2:+85347718 None:intergenic
GAGACGATTTGTTTGAAGGA+AGG 0.460863 3.2:+85348295 None:intergenic
ATGCTCAGCATCATCGTGGC+TGG 0.463381 3.2:-85348496 MS.gene015095:CDS
ATTGAGAAAATTCTTCCTCC+AGG 0.468590 3.2:-85347875 MS.gene015095:CDS
AGGAGAAGGATCATTGACTA+TGG 0.472377 3.2:+85348381 None:intergenic
TGGTCATGGAAACCTTGATT+AGG 0.475061 3.2:+85348593 None:intergenic
TGCTGTGGCGGCTCCGGTGA+GGG 0.488839 3.2:+85348752 None:intergenic
AATAAACTGCTTCTCTCCAT+TGG 0.490699 3.2:+85347709 None:intergenic
TGACGAATGATGTGAATTCA+TGG 0.493920 3.2:-85347922 MS.gene015095:CDS
GTCAATGATCCTTCTCCTGC+CGG 0.494111 3.2:-85348376 MS.gene015095:CDS
GCGGCTCCGGTGAGGGCGAG+GGG 0.495260 3.2:+85348759 None:intergenic
ACTGTCTTCTTTGGTTGAGA+AGG 0.496004 3.2:-85347585 MS.gene015095:CDS
CTCCTTTGTTATAAGGAAGA+CGG 0.496566 3.2:+85347660 None:intergenic
TACCTTGTTCTTGAAATCTA+CGG 0.497341 3.2:+85348246 None:intergenic
GAACGAGAGCGGTTGTAAGA+AGG 0.500652 3.2:+85348685 None:intergenic
GAGACGCAAGGCCCACCGGC+AGG 0.502939 3.2:+85348361 None:intergenic
GAAAGCCTTGTGAAATATCT+GGG 0.503802 3.2:+85347364 None:intergenic
CAAATGGTTGGCCATGGTCA+TGG 0.504652 3.2:+85348579 None:intergenic
TTTCTCTTTGTCTAAAATGG+TGG 0.506008 3.2:-85347423 MS.gene015095:CDS
TTTCTTCCAAATGCAAGAGA+TGG 0.506976 3.2:-85347608 MS.gene015095:CDS
CATCATCGTGGCTGGATCTG+AGG 0.508886 3.2:-85348488 MS.gene015095:CDS
TCTCTAATTAGAAATAAGAA+AGG 0.509129 3.2:+85347236 None:intergenic
AAAAGAGACGCAAGGCCCAC+CGG 0.509729 3.2:+85348357 None:intergenic
TGGGCCAAAACGGAGACAAA+TGG 0.522116 3.2:-85347902 MS.gene015095:CDS
GTGAATTTGTTGACTGACCC+TGG 0.527918 3.2:+85347857 None:intergenic
TGAAAGGAGATGAGAGGCAA+AGG 0.527949 3.2:+85348405 None:intergenic
GAGGGCGAGGGGATAGAGAA+TGG 0.531179 3.2:+85348770 None:intergenic
TGTGAATTCATGGGCCAAAA+CGG 0.535516 3.2:-85347912 MS.gene015095:CDS
CTCTAATTAGAAATAAGAAA+GGG 0.536391 3.2:+85347237 None:intergenic
CAAAAGCTTCTCGACTTCCT+CGG 0.543373 3.2:-85348454 MS.gene015095:CDS
TTGAGAAAATTCTTCCTCCA+GGG 0.549599 3.2:-85347874 MS.gene015095:CDS
ATCATCGTGGCTGGATCTGA+GGG 0.551620 3.2:-85348487 MS.gene015095:CDS
CTCCGTCTTCCTTATAACAA+AGG 0.551836 3.2:-85347662 MS.gene015095:CDS
TTCTCTCCATTGGTCATGAA+GGG 0.552364 3.2:+85347719 None:intergenic
GCTCTCGTTCTCCTGTGCCA+CGG 0.552729 3.2:-85348672 MS.gene015095:CDS
CGCTAAGAAAACTTAACAAG+AGG 0.552754 3.2:+85347170 None:intergenic
AATGATCCTTCTCCTGCCGG+TGG 0.553899 3.2:-85348373 MS.gene015095:CDS
AGAAATTTCTGATATGCTGA+AGG 0.554669 3.2:-85347465 MS.gene015095:CDS
TTGTTCAACCCATAGGCCAT+TGG 0.557077 3.2:+85348333 None:intergenic
CATTGTACTTCAAAGGGGTG+TGG 0.558354 3.2:-85347814 MS.gene015095:CDS
AAAGGAGTTGAGATGATCAA+TGG 0.558689 3.2:+85348423 None:intergenic
TAGAAATAAGAAAGGGTGGT+CGG 0.562218 3.2:+85347244 None:intergenic
GGAGAGCAAATGGTTGGCCA+TGG 0.562406 3.2:+85348573 None:intergenic
GAGCGGTTGTAAGAAGGAGG+TGG 0.563038 3.2:+85348691 None:intergenic
ATGCTGTGGCGGCTCCGGTG+AGG 0.567495 3.2:+85348751 None:intergenic
AATGAGAATGTTGTTGCGGC+TGG 0.567626 3.2:+85348647 None:intergenic
AAGGTTCCCTTCATGACCAA+TGG 0.569018 3.2:-85347725 MS.gene015095:CDS
TGGAAACCTTGATTAGGTCA+TGG 0.569544 3.2:+85348599 None:intergenic
TTCCGTAGATTTCAAGAACA+AGG 0.570481 3.2:-85348248 MS.gene015095:intron
ATGATCCTTCTCCTGCCGGT+GGG 0.572827 3.2:-85348372 MS.gene015095:CDS
TATGAACCATGACCTAATCA+AGG 0.575160 3.2:-85348605 MS.gene015095:CDS
TTGTTTGGAGAGCAAATGGT+TGG 0.575521 3.2:+85348567 None:intergenic
AAAGGGATTGTTCAACCCAT+AGG 0.579225 3.2:+85348326 None:intergenic
GATCATTGACTATGGCTGAA+AGG 0.579763 3.2:+85348389 None:intergenic
GAAATCTACGGAAGACAAAG+TGG 0.582565 3.2:+85348258 None:intergenic
TGTTGCTTGTCTCGTGGCAG+AGG 0.585574 3.2:+85348724 None:intergenic
TGTTGTTGCGGCTGGTCCCG+TGG 0.588060 3.2:+85348655 None:intergenic
AGCCACGATGATGCTGAGCA+TGG 0.595040 3.2:+85348498 None:intergenic
AGAAGGAACTGAAGCTGCTG+CGG 0.604436 3.2:-85347327 MS.gene015095:CDS
CGATGATGCTGAGCATGGAG+TGG 0.617758 3.2:+85348503 None:intergenic
CGAGAGCGGTTGTAAGAAGG+AGG 0.618378 3.2:+85348688 None:intergenic
GCAGAGGAGTGATGCTGTGG+CGG 0.619829 3.2:+85348740 None:intergenic
TGTTTCCCAGATATTTCACA+AGG 0.624736 3.2:-85347369 MS.gene015095:CDS
TCATAATGGAAGCTCAGTCA+AGG 0.629742 3.2:-85347744 MS.gene015095:CDS
GTGGCAGAGGAGTGATGCTG+TGG 0.644396 3.2:+85348737 None:intergenic
CTTTAGAAAGACGAAACTTG+TGG 0.653242 3.2:+85347537 None:intergenic
GACGAATGATGTGAATTCAT+GGG 0.653709 3.2:-85347921 MS.gene015095:CDS
AATCAAGGTTTCCATGACCA+TGG 0.656656 3.2:-85348590 MS.gene015095:CDS
AGCATGGAGTGGATTAACAA+TGG 0.657918 3.2:+85348514 None:intergenic
CTCCATGCTCAGCATCATCG+TGG 0.659516 3.2:-85348500 MS.gene015095:CDS
TATGGCTGAAAGGAGATGAG+AGG 0.661161 3.2:+85348399 None:intergenic
GTGGCACAGGAGAACGAGAG+CGG 0.663796 3.2:+85348674 None:intergenic
AGAGAATGAGAATGTTGTTG+CGG 0.669476 3.2:+85348643 None:intergenic
TAATTAGAAATAAGAAAGGG+TGG 0.671650 3.2:+85347240 None:intergenic
GAATGATGTTGCTTGTCTCG+TGG 0.678057 3.2:+85348718 None:intergenic
TCGGCTACAAAGTCTAGTCG+AGG 0.687215 3.2:+85347263 None:intergenic
TAATGCATTGTACTTCAAAG+GGG 0.696482 3.2:-85347819 MS.gene015095:CDS
CCATTGGCAAAAGAGACGCA+AGG 0.707823 3.2:+85348349 None:intergenic
ATAACAAAGGAGAAGATACG+CGG 0.735798 3.2:-85347649 MS.gene015095:CDS
AATTTGTTGACTGACCCTGG+AGG 0.835836 3.2:+85347860 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAATAAATTATGCAAA+TGG + chr3.2:85347952-85347971 None:intergenic 10.0%
!! TCATAACATAAATAATTTAG+AGG - chr3.2:85347817-85347836 MS.gene015095:CDS 15.0%
!!! CTTTATTTTCTGTTAATTAA+TGG - chr3.2:85347761-85347780 MS.gene015095:CDS 15.0%
!!! GCATACTTTATTTTAAAATT+TGG - chr3.2:85347982-85348001 MS.gene015095:intron 15.0%
!! CTCTAATTAGAAATAAGAAA+GGG + chr3.2:85348716-85348735 None:intergenic 20.0%
!! TCTCTAATTAGAAATAAGAA+AGG + chr3.2:85348717-85348736 None:intergenic 20.0%
!!! AAAATTTGGTATGATTTTGT+AGG - chr3.2:85347996-85348015 MS.gene015095:intron 20.0%
!!! TATGATTTTCACATTCATAA+TGG - chr3.2:85348192-85348211 MS.gene015095:intron 20.0%
! AATTGAAGCATATAATTCAG+AGG - chr3.2:85347851-85347870 MS.gene015095:CDS 25.0%
! AGATTTCTCTTTGTCTAAAA+TGG - chr3.2:85348524-85348543 MS.gene015095:CDS 25.0%
! TAATTAGAAATAAGAAAGGG+TGG + chr3.2:85348713-85348732 None:intergenic 25.0%
!!! CTTTTCTTTAAGTTCTTGTT+TGG + chr3.2:85347401-85347420 None:intergenic 25.0%
AACAGAAAATAAAGCAGCTA+GGG + chr3.2:85347755-85347774 None:intergenic 30.0%
AGAAATTTCTGATATGCTGA+AGG - chr3.2:85348485-85348504 MS.gene015095:CDS 30.0%
AGTACACTAATTGATTGTGA+AGG + chr3.2:85347792-85347811 None:intergenic 30.0%
CATATAATTCAGAGGACATA+TGG - chr3.2:85347859-85347878 MS.gene015095:CDS 30.0%
CTAATGCATTGTACTTCAAA+GGG - chr3.2:85348130-85348149 MS.gene015095:intron 30.0%
GAAATATCTGGGAAACAAAA+TGG + chr3.2:85348578-85348597 None:intergenic 30.0%
GAATCAACAATTTGATGCTT+CGG - chr3.2:85348158-85348177 MS.gene015095:intron 30.0%
GTACACTAATTGATTGTGAA+GGG + chr3.2:85347791-85347810 None:intergenic 30.0%
GTTAGTAAAGAACGACTTAA+AGG + chr3.2:85347908-85347927 None:intergenic 30.0%
TAACAGAAAATAAAGCAGCT+AGG + chr3.2:85347756-85347775 None:intergenic 30.0%
TAATGCATTGTACTTCAAAG+GGG - chr3.2:85348131-85348150 MS.gene015095:intron 30.0%
TACCTTGTTCTTGAAATCTA+CGG + chr3.2:85347707-85347726 None:intergenic 30.0%
TCAAATGAAACCTTGAATCT+TGG + chr3.2:85348462-85348481 None:intergenic 30.0%
TTCATTGAAGTGAATGAAGA+AGG - chr3.2:85348606-85348625 MS.gene015095:CDS 30.0%
! AATCGTCTCTACTGATTATA+AGG - chr3.2:85347669-85347688 MS.gene015095:CDS 30.0%
! GAGGAAGAATTTTCTCAATA+AGG + chr3.2:85348074-85348093 None:intergenic 30.0%
! GTATCTTCTCCTTTGTTATA+AGG + chr3.2:85348300-85348319 None:intergenic 30.0%
! TTTCTCTTTGTCTAAAATGG+TGG - chr3.2:85348527-85348546 MS.gene015095:CDS 30.0%
!! AAATGGTCTTGATTTTGATC+TGG + chr3.2:85348561-85348580 None:intergenic 30.0%
AAAGGAGTTGAGATGATCAA+TGG + chr3.2:85347530-85347549 None:intergenic 35.0%
AATAAACTGCTTCTCTCCAT+TGG + chr3.2:85348244-85348263 None:intergenic 35.0%
AATGGAGAGAAGCAGTTTAT+TGG - chr3.2:85348243-85348262 MS.gene015095:intron 35.0%
AGAGAATGAGAATGTTGTTG+CGG + chr3.2:85347310-85347329 None:intergenic 35.0%
ATAACAAAGGAGAAGATACG+CGG - chr3.2:85348301-85348320 MS.gene015095:CDS 35.0%
ATTGAGAAAATTCTTCCTCC+AGG - chr3.2:85348075-85348094 MS.gene015095:intron 35.0%
CTTCAGAATTCCAAGATTCA+AGG - chr3.2:85348449-85348468 MS.gene015095:CDS 35.0%
GACGAATGATGTGAATTCAT+GGG - chr3.2:85348029-85348048 MS.gene015095:intron 35.0%
GCTAATGCATTGTACTTCAA+AGG - chr3.2:85348129-85348148 MS.gene015095:intron 35.0%
TAGAAATAAGAAAGGGTGGT+CGG + chr3.2:85348709-85348728 None:intergenic 35.0%
TATGAACCATGACCTAATCA+AGG - chr3.2:85347345-85347364 MS.gene015095:CDS 35.0%
TCCAACAAAAAGGATCGTTT+CGG + chr3.2:85348748-85348767 None:intergenic 35.0%
TGACGAATGATGTGAATTCA+TGG - chr3.2:85348028-85348047 MS.gene015095:intron 35.0%
TGTTTCCCAGATATTTCACA+AGG - chr3.2:85348581-85348600 MS.gene015095:CDS 35.0%
TTCCGTAGATTTCAAGAACA+AGG - chr3.2:85347702-85347721 MS.gene015095:CDS 35.0%
TTGAGAAAATTCTTCCTCCA+GGG - chr3.2:85348076-85348095 MS.gene015095:intron 35.0%
TTTCTTCCAAATGCAAGAGA+TGG - chr3.2:85348342-85348361 MS.gene015095:CDS 35.0%
! AGTAGAGACGATTTGTTTGA+AGG + chr3.2:85347662-85347681 None:intergenic 35.0%
! CTCCTTTGTTATAAGGAAGA+CGG + chr3.2:85348293-85348312 None:intergenic 35.0%
! CTTTAGAAAGACGAAACTTG+TGG + chr3.2:85348416-85348435 None:intergenic 35.0%
! GAAAGCCTTGTGAAATATCT+GGG + chr3.2:85348589-85348608 None:intergenic 35.0%
! TGAAAGCCTTGTGAAATATC+TGG + chr3.2:85348590-85348609 None:intergenic 35.0%
AAAATAAAGCAGCTAGGGCA+TGG + chr3.2:85347750-85347769 None:intergenic 40.0%
AAAGGGATTGTTCAACCCAT+AGG + chr3.2:85347627-85347646 None:intergenic 40.0%
AATCAAGGTTTCCATGACCA+TGG - chr3.2:85347360-85347379 MS.gene015095:CDS 40.0%
ACTGTCTTCTTTGGTTGAGA+AGG - chr3.2:85348365-85348384 MS.gene015095:CDS 40.0%
CTCCGTCTTCCTTATAACAA+AGG - chr3.2:85348288-85348307 MS.gene015095:CDS 40.0%
GAAATCTACGGAAGACAAAG+TGG + chr3.2:85347695-85347714 None:intergenic 40.0%
GAAGGAAGGTTGAAGAGAAA+GGG + chr3.2:85347644-85347663 None:intergenic 40.0%
TCTGATATGCTGAAGGAGTT+AGG - chr3.2:85348492-85348511 MS.gene015095:CDS 40.0%
TGAAGGAAGGTTGAAGAGAA+AGG + chr3.2:85347645-85347664 None:intergenic 40.0%
TGAGCACATGTCCAACAAAA+AGG + chr3.2:85348758-85348777 None:intergenic 40.0%
TGGAAACCTTGATTAGGTCA+TGG + chr3.2:85347354-85347373 None:intergenic 40.0%
TGGTCATGGAAACCTTGATT+AGG + chr3.2:85347360-85347379 None:intergenic 40.0%
TGTGAATTCATGGGCCAAAA+CGG - chr3.2:85348038-85348057 MS.gene015095:intron 40.0%
TTCTCTCCATTGGTCATGAA+GGG + chr3.2:85348234-85348253 None:intergenic 40.0%
TTGTTTGGAGAGCAAATGGT+TGG + chr3.2:85347386-85347405 None:intergenic 40.0%
! ACCGAAACGATCCTTTTTGT+TGG - chr3.2:85348744-85348763 MS.gene015095:CDS 40.0%
! AGGAGAAGGATCATTGACTA+TGG + chr3.2:85347572-85347591 None:intergenic 40.0%
! GAGACGATTTGTTTGAAGGA+AGG + chr3.2:85347658-85347677 None:intergenic 40.0%
! GATCATTGACTATGGCTGAA+AGG + chr3.2:85347564-85347583 None:intergenic 40.0%
! GTTCTTGTTTGGAGAGCAAA+TGG + chr3.2:85347390-85347409 None:intergenic 40.0%
! TCATAATGGAAGCTCAGTCA+AGG - chr3.2:85348206-85348225 MS.gene015095:intron 40.0%
! TCGAGAAGCTTTTGATGTGT+GGG + chr3.2:85347490-85347509 None:intergenic 40.0%
!! AAGAGATGGACTGTCTTCTT+TGG - chr3.2:85348356-85348375 MS.gene015095:CDS 40.0%
!! AGCATGGAGTGGATTAACAA+TGG + chr3.2:85347439-85347458 None:intergenic 40.0%
!! CGTCTTTCTAAAGTCGAAGT+AGG - chr3.2:85348423-85348442 MS.gene015095:CDS 40.0%
AAGGTTCCCTTCATGACCAA+TGG - chr3.2:85348225-85348244 MS.gene015095:intron 45.0%
AATGAGAATGTTGTTGCGGC+TGG + chr3.2:85347306-85347325 None:intergenic 45.0%
CAAAAGCTTCTCGACTTCCT+CGG - chr3.2:85347496-85347515 MS.gene015095:CDS 45.0%
CATTGTACTTCAAAGGGGTG+TGG - chr3.2:85348136-85348155 MS.gene015095:intron 45.0%
CTTCTCTCCATTGGTCATGA+AGG + chr3.2:85348235-85348254 None:intergenic 45.0%
GACAGTCCATCTCTTGCATT+TGG + chr3.2:85348351-85348370 None:intergenic 45.0%
GTCTTCTTTGGTTGAGAAGG+TGG - chr3.2:85348368-85348387 MS.gene015095:CDS 45.0%
TATGGCTGAAAGGAGATGAG+AGG + chr3.2:85347554-85347573 None:intergenic 45.0%
TGAAAGGAGATGAGAGGCAA+AGG + chr3.2:85347548-85347567 None:intergenic 45.0%
TTGTTCAACCCATAGGCCAT+TGG + chr3.2:85347620-85347639 None:intergenic 45.0%
! AAGGCCATTTGTCTCCGTTT+TGG + chr3.2:85348055-85348074 None:intergenic 45.0%
! CTCTTTTGCCAATGGCCTAT+GGG - chr3.2:85347609-85347628 MS.gene015095:CDS 45.0%
! GAATGATGTTGCTTGTCTCG+TGG + chr3.2:85347235-85347254 None:intergenic 45.0%
! GTCGAGAAGCTTTTGATGTG+TGG + chr3.2:85347491-85347510 None:intergenic 45.0%
! TCTCTTTTGCCAATGGCCTA+TGG - chr3.2:85347608-85347627 MS.gene015095:CDS 45.0%
!! AATTTGTTGACTGACCCTGG+AGG + chr3.2:85348093-85348112 None:intergenic 45.0%
!! GATCAATGGATTTTGAGCCG+AGG + chr3.2:85347516-85347535 None:intergenic 45.0%
!! GTGAATTTGTTGACTGACCC+TGG + chr3.2:85348096-85348115 None:intergenic 45.0%
AGAAGGAACTGAAGCTGCTG+CGG - chr3.2:85348623-85348642 MS.gene015095:CDS 50.0%
ATCATCGTGGCTGGATCTGA+GGG - chr3.2:85347463-85347482 MS.gene015095:CDS 50.0%
CCATTGGCAAAAGAGACGCA+AGG + chr3.2:85347604-85347623 None:intergenic 50.0%
GAACGAGAGCGGTTGTAAGA+AGG + chr3.2:85347268-85347287 None:intergenic 50.0%
GTCAATGATCCTTCTCCTGC+CGG - chr3.2:85347574-85347593 MS.gene015095:CDS 50.0%
TCGGCTACAAAGTCTAGTCG+AGG + chr3.2:85348690-85348709 None:intergenic 50.0%
TGGGCCAAAACGGAGACAAA+TGG - chr3.2:85348048-85348067 MS.gene015095:intron 50.0%
! CAAATGGTTGGCCATGGTCA+TGG + chr3.2:85347374-85347393 None:intergenic 50.0%
! CCTTGCGTCTCTTTTGCCAA+TGG - chr3.2:85347601-85347620 MS.gene015095:CDS 50.0%
AAAAGAGACGCAAGGCCCAC+CGG + chr3.2:85347596-85347615 None:intergenic 55.0%
AATGATCCTTCTCCTGCCGG+TGG - chr3.2:85347577-85347596 MS.gene015095:CDS 55.0%
AGCCACGATGATGCTGAGCA+TGG + chr3.2:85347455-85347474 None:intergenic 55.0%
ATGATCCTTCTCCTGCCGGT+GGG - chr3.2:85347578-85347597 MS.gene015095:CDS 55.0%
ATGCTCAGCATCATCGTGGC+TGG - chr3.2:85347454-85347473 MS.gene015095:CDS 55.0%
CATCATCGTGGCTGGATCTG+AGG - chr3.2:85347462-85347481 MS.gene015095:CDS 55.0%
CGAGAGCGGTTGTAAGAAGG+AGG + chr3.2:85347265-85347284 None:intergenic 55.0%
CGATGATGCTGAGCATGGAG+TGG + chr3.2:85347450-85347469 None:intergenic 55.0%
CGGTTGTAAGAAGGAGGTGG+AGG + chr3.2:85347259-85347278 None:intergenic 55.0%
CTCCATGCTCAGCATCATCG+TGG - chr3.2:85347450-85347469 MS.gene015095:CDS 55.0%
GAGCGGTTGTAAGAAGGAGG+TGG + chr3.2:85347262-85347281 None:intergenic 55.0%
! GGAGAGCAAATGGTTGGCCA+TGG + chr3.2:85347380-85347399 None:intergenic 55.0%
! TGTTGCTTGTCTCGTGGCAG+AGG + chr3.2:85347229-85347248 None:intergenic 55.0%
CTCTCGTTCTCCTGTGCCAC+GGG - chr3.2:85347279-85347298 MS.gene015095:CDS 60.0%
GAGGGCGAGGGGATAGAGAA+TGG + chr3.2:85347183-85347202 None:intergenic 60.0%
GCAGAGGAGTGATGCTGTGG+CGG + chr3.2:85347213-85347232 None:intergenic 60.0%
GCTCTCGTTCTCCTGTGCCA+CGG - chr3.2:85347278-85347297 MS.gene015095:CDS 60.0%
GTGGCACAGGAGAACGAGAG+CGG + chr3.2:85347279-85347298 None:intergenic 60.0%
GTGGCAGAGGAGTGATGCTG+TGG + chr3.2:85347216-85347235 None:intergenic 60.0%
CTCTATCCCCTCGCCCTCAC+CGG - chr3.2:85347185-85347204 MS.gene015095:CDS 65.0%
GAGTGATGCTGTGGCGGCTC+CGG + chr3.2:85347207-85347226 None:intergenic 65.0%
! TGTTGTTGCGGCTGGTCCCG+TGG + chr3.2:85347298-85347317 None:intergenic 65.0%
ATGCTGTGGCGGCTCCGGTG+AGG + chr3.2:85347202-85347221 None:intergenic 70.0%
CAAGGCCCACCGGCAGGAGA+AGG + chr3.2:85347586-85347605 None:intergenic 70.0%
TGCTGTGGCGGCTCCGGTGA+GGG + chr3.2:85347201-85347220 None:intergenic 70.0%
GAGACGCAAGGCCCACCGGC+AGG + chr3.2:85347592-85347611 None:intergenic 75.0%
! TGCGGCTGGTCCCGTGGCAC+AGG + chr3.2:85347292-85347311 None:intergenic 75.0%
! GCGGCTCCGGTGAGGGCGAG+GGG + chr3.2:85347194-85347213 None:intergenic 80.0%
! GGCGGCTCCGGTGAGGGCGA+GGG + chr3.2:85347195-85347214 None:intergenic 80.0%
! TGGCGGCTCCGGTGAGGGCG+AGG + chr3.2:85347196-85347215 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 85347172 85348800 85347172 ID=MS.gene015095
chr3.2 mRNA 85347172 85348800 85347172 ID=MS.gene015095.t1;Parent=MS.gene015095
chr3.2 exon 85348249 85348800 85348249 ID=MS.gene015095.t1.exon1;Parent=MS.gene015095.t1
chr3.2 CDS 85348249 85348800 85348249 ID=cds.MS.gene015095.t1;Parent=MS.gene015095.t1
chr3.2 exon 85347172 85347954 85347172 ID=MS.gene015095.t1.exon2;Parent=MS.gene015095.t1
chr3.2 CDS 85347172 85347954 85347172 ID=cds.MS.gene015095.t1;Parent=MS.gene015095.t1
Gene Sequence

>MS.gene015095

ATGGAACGCCATTCTCTATCCCCTCGCCCTCACCGGAGCCGCCACAGCATCACTCCTCTGCCACGAGACAAGCAACATCATTCTCCTCCACCTCCTTCTTACAACCGCTCTCGTTCTCCTGTGCCACGGGACCAGCCGCAACAACATTCTCATTCTCTAACTAATAGAAGATATATGAACCATGACCTAATCAAGGTTTCCATGACCATGGCCAACCATTTGCTCTCCAAACAAGAACTTAAAGAAAAGAATGTTGTGTTTTCGCCATTGTTAATCCACTCCATGCTCAGCATCATCGTGGCTGGATCTGAGGGTCCCACACATCAAAAGCTTCTCGACTTCCTCGGCTCAAAATCCATTGATCATCTCAACTCCTTTGCCTCTCATCTCCTTTCAGCCATAGTCAATGATCCTTCTCCTGCCGGTGGGCCTTGCGTCTCTTTTGCCAATGGCCTATGGGTTGAACAATCCCTTTCTCTTCAACCTTCCTTCAAACAAATCGTCTCTACTGATTATAAGGCCACTTTGTCTTCCGTAGATTTCAAGAACAAGGTATTTTTCTTTTTCACTACAATCCATGCCCTAGCTGCTTTATTTTCTGTTAATTAATGGATCTCCCTTCACAATCAATTAGTGTACTTTATTTCATAACATAAATAATTTAGAGGACATGTTTTACAATTGAAGCATATAATTCAGAGGACATATGGTTTTACAATTGAAGCATAAATAACCTTTAAGTCGTTCTTTACTAACAAGTATTGCTTTTATTTTTATCCATTTGCATAATTTATTTATAAATTTGATGATGCATACTTTATTTTAAAATTTGGTATGATTTTGTAGGCTGATAAAGTGACGAATGATGTGAATTCATGGGCCAAAACGGAGACAAATGGCCTTATTGAGAAAATTCTTCCTCCAGGGTCAGTCAACAAATTCACAAAACTCATCTTTGCTAATGCATTGTACTTCAAAGGGGTGTGGAATCAACAATTTGATGCTTCGGAAACAAAAGACTATGATTTTCACATTCATAATGGAAGCTCAGTCAAGGTTCCCTTCATGACCAATGGAGAGAAGCAGTTTATTGGAGCTTTTGATGATTTTAAAGTTCTCCGTCTTCCTTATAACAAAGGAGAAGATACGCGGAAATTCTCTATGTACATTTTTCTTCCAAATGCAAGAGATGGACTGTCTTCTTTGGTTGAGAAGGTGGCTTCTGAATCTGAGTTACTACACCACAAGTTTCGTCTTTCTAAAGTCGAAGTAGGTGACTTCAGAATTCCAAGATTCAAGGTTTCATTTGAACAAGAAATTTCTGATATGCTGAAGGAGTTAGGAGTGAGTTTAGATTTCTCTTTGTCTAAAATGGTGGACTCTCTTCCAGATCAAAATCAAGACCATTTTGTTTCCCAGATATTTCACAAGGCTTTCATTGAAGTGAATGAAGAAGGAACTGAAGCTGCTGCGGTCACTGCTAATACTGTTGAAATCAAGAGCTTTAGTATAAGTCCTCGACTAGACTTTGTAGCCGACCACCCTTTCTTATTTCTAATTAGAGAAGATTTGACCGAAACGATCCTTTTTGTTGGACATGTGCTCAATCCTCTTGTTAAGTTTTCTTAG
Protein sequence

>MS.gene015095.t1

MERHSLSPRPHRSRHSITPLPRDKQHHSPPPPSYNRSRSPVPRDQPQQHSHSLTNRRYMNHDLIKVSMTMANHLLSKQELKEKNVVFSPLLIHSMLSIIVAGSEGPTHQKLLDFLGSKSIDHLNSFASHLLSAIVNDPSPAGGPCVSFANGLWVEQSLSLQPSFKQIVSTDYKATLSSVDFKNKADKVTNDVNSWAKTETNGLIEKILPPGSVNKFTKLIFANALYFKGVWNQQFDASETKDYDFHIHNGSSVKVPFMTNGEKQFIGAFDDFKVLRLPYNKGEDTRKFSMYIFLPNARDGLSSLVEKVASESELLHHKFRLSKVEVGDFRIPRFKVSFEQEISDMLKELGVSLDFSLSKMVDSLPDQNQDHFVSQIFHKAFIEVNEEGTEAAAVTANTVEIKSFSISPRLDFVADHPFLFLIREDLTETILFVGHVLNPLVKFS