AlfalfaGEDB | MS.gene01558

M. sativa cultivar XinJiangDaYe / MS.gene01558

KEGG: -
GO: GO:0005575 GO:0005615 GO:0110165
PFAM: PF01190.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01558.t1	XP_013462553.1	97.6	168	4	0	1	168	1	168	1.60E-87	332

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01558.t1	O49813	56.4	163	69	2	1	162	1	162	2.0e-46	186.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01558.t1	B7FGN2	97.6	168	4	0	1	168	1	168	1.1e-87	332.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene002573	MS.gene01558	PPI
MS.gene54304	MS.gene01558	PPI
MS.gene01558	MS.gene54304	PPI
MS.gene01558	MS.gene004245	PPI
MS.gene01558	MS.gene002573	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01558.t1	MTR_2g016020	97.619	168	4	0	1	168	1	168	2.55e-120	336
MS.gene01558.t1	MTR_2g077940	35.928	167	88	4	8	161	11	171	2.81e-27	100
MS.gene01558.t1	MTR_2g077960	36.025	161	90	4	8	161	11	165	4.50e-27	100
MS.gene01558.t1	MTR_3g087540	34.091	132	79	5	28	155	25	152	6.91e-22	86.7
MS.gene01558.t1	MTR_3g073990	32.911	158	102	2	1	157	1	155	1.28e-21	86.3
MS.gene01558.t1	MTR_0390s0010	35.032	157	92	2	8	161	11	160	2.86e-19	80.1
MS.gene01558.t1	MTR_0786s0020	34.395	157	93	2	8	161	11	160	2.01e-18	77.8
MS.gene01558.t1	MTR_2g450190	33.758	157	91	3	8	161	11	157	3.47e-16	72.0
MS.gene01558.t1	MTR_0390s0040	33.758	157	91	3	8	161	11	157	3.47e-16	72.0
MS.gene01558.t1	MTR_2g077080	31.915	141	87	2	23	161	31	164	4.76e-14	66.2
MS.gene01558.t1	MTR_1678s0010	28.082	146	95	5	28	168	38	178	1.37e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01558.t1	AT1G29140	49.412	170	80	4	1	164	1	170	2.26e-50	159
MS.gene01558.t1	AT5G45880	53.571	140	63	2	27	164	34	173	8.99e-50	158
MS.gene01558.t1	AT4G18596	47.953	171	82	4	1	164	1	171	1.16e-49	158
MS.gene01558.t1	AT4G18596	47.953	171	82	4	1	164	47	217	6.37e-49	157
MS.gene01558.t1	AT4G08685	39.241	158	89	5	8	162	6	159	1.88e-31	111
MS.gene01558.t1	AT5G10130	37.349	166	94	3	1	162	1	160	6.64e-28	102
MS.gene01558.t1	AT1G78040	32.143	168	103	5	1	162	1	163	4.30e-21	85.1
MS.gene01558.t1	AT1G78040	32.143	168	103	5	1	162	1	163	4.30e-21	85.1
MS.gene01558.t1	AT1G78040	32.143	168	103	5	1	162	1	163	4.30e-21	85.1

Find 42 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAGAACCTCACCACATTT+AGG	0.300765	2.2:+67531943	None:intergenic
CGGTCAATCTGGTGATGAAT+TGG	0.316647	2.2:+67532625	None:intergenic
GTGGAATGCAAAGAAGAAAA+TGG	0.335509	2.2:-67532213	MS.gene01558:CDS
AGAGGGCTAGCAGCACGAAC+TGG	0.354642	2.2:+67531991	None:intergenic
CCTGAACGCTTCTTTGTAGA+AGG	0.366196	2.2:-67532675	MS.gene01558:CDS
AAATACAGTGTGGAGATAGA+TGG	0.398924	2.2:-67532147	MS.gene01558:CDS
GTTCGTGCTGCTAGCCCTCT+TGG	0.402448	2.2:-67531988	MS.gene01558:CDS
TAGGTAGACGCTCTTTCTTA+AGG	0.405456	2.2:+67531962	None:intergenic
TTCAATGAACTCGGTCAATC+TGG	0.407937	2.2:+67532614	None:intergenic
TACAAAGAAGCGTTCAGGCT+TGG	0.439377	2.2:+67532680	None:intergenic
GAACTTGGAATTAATGAAGA+TGG	0.440677	2.2:-67531919	MS.gene01558:CDS
ACCTCTGAGCAATCTTTCCT+TGG	0.443518	2.2:+67532081	None:intergenic
TGATCCTGGCAGCTTGTTGT+AGG	0.452351	2.2:+67532043	None:intergenic
GAAGCAACAACAGATAAATC+AGG	0.454577	2.2:-67532171	MS.gene01558:CDS
TAATGAAGATGGAACTGAAA+AGG	0.471543	2.2:-67531908	MS.gene01558:CDS
CCTTCTACAAAGAAGCGTTC+AGG	0.474728	2.2:+67532675	None:intergenic
GTTGTTTGTGACACTGATCC+TGG	0.479217	2.2:+67532029	None:intergenic
GGAATAAGCCGCACCTAAGA+AGG	0.479861	2.2:+67532701	None:intergenic
GAGGGCTAGCAGCACGAACT+GGG	0.491624	2.2:+67531992	None:intergenic
AGATTGACCGAGTTCATTGA+AGG	0.500832	2.2:-67532612	MS.gene01558:intron
GGTGAGGTTCTCAAAGAACT+TGG	0.517638	2.2:-67531934	MS.gene01558:CDS
GGTCAATCTGGTGATGAATT+GGG	0.518245	2.2:+67532626	None:intergenic
CTTCACATGTTTCATCCTCA+TGG	0.533134	2.2:+67532121	None:intergenic
TTATTCTTTGCAAGAGTCCA+AGG	0.534437	2.2:-67532098	MS.gene01558:CDS
CTCACCTACAACAAGCTGCC+AGG	0.535934	2.2:-67532047	MS.gene01558:CDS
AAAAGCAAAGGGTGAAGACA+AGG	0.570670	2.2:+67532727	None:intergenic
TGAAGGTGCAACGGTGCGTG+TGG	0.602260	2.2:-67532232	MS.gene01558:intron
CTTTCTTAAGGAAACCAAGA+GGG	0.608256	2.2:+67531974	None:intergenic
ATCAGTGTCACAAACAACAA+TGG	0.609110	2.2:-67532024	MS.gene01558:CDS
AAGCAAAGGGTGAAGACAAG+GGG	0.617019	2.2:+67532729	None:intergenic
TGAAGATGGAACTGAAAAGG+AGG	0.627791	2.2:-67531905	MS.gene01558:CDS
GTACGTACCTTCAATGAACT+CGG	0.638216	2.2:+67532605	None:intergenic
AAAGAGCGTCTACCTAAATG+TGG	0.642536	2.2:-67531955	MS.gene01558:CDS
AGGGCTAGCAGCACGAACTG+GGG	0.655258	2.2:+67531993	None:intergenic
TATATGTAATGAAGGTGCAA+CGG	0.656510	2.2:-67532241	MS.gene01558:intron
AAAGCAAAGGGTGAAGACAA+GGG	0.660463	2.2:+67532728	None:intergenic
GCGTCTACCTAAATGTGGTG+AGG	0.675591	2.2:-67531950	MS.gene01558:CDS
TCTTTCTTAAGGAAACCAAG+AGG	0.685975	2.2:+67531973	None:intergenic
TAAATCAGGAAAATACAGTG+TGG	0.693438	2.2:-67532157	MS.gene01558:CDS
TCCAAGGAAAGATTGCTCAG+AGG	0.698789	2.2:-67532082	MS.gene01558:CDS
AGCAAAGGGTGAAGACAAGG+GGG	0.721824	2.2:+67532730	None:intergenic
GGAGATAGATGGAGACCATG+AGG	0.745188	2.2:-67532136	MS.gene01558:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATAATCTGAATTTTACTTTT+AGG	+	chr2.2:67532246-67532265	None:intergenic	15.0%
!!!	GACATAAATAATAATTTTTG+TGG	-	chr2.2:67532380-67532399	MS.gene01558:intron	15.0%
!	ACACATTTATTACTAAGAAG+CGG	-	chr2.2:67532306-67532325	MS.gene01558:intron	25.0%
!	TAAGAAGGATAAAAAGCAAA+GGG	+	chr2.2:67531934-67531953	None:intergenic	25.0%
!!!	TTTTACTTTTAGGTATTACG+AGG	+	chr2.2:67532236-67532255	None:intergenic	25.0%
	CTAAGAAGGATAAAAAGCAA+AGG	+	chr2.2:67531935-67531954	None:intergenic	30.0%
	GAACTTGGAATTAATGAAGA+TGG	-	chr2.2:67532728-67532747	MS.gene01558:CDS	30.0%
	TAAATCAGGAAAATACAGTG+TGG	-	chr2.2:67532490-67532509	MS.gene01558:intron	30.0%
	TAATGAAGATGGAACTGAAA+AGG	-	chr2.2:67532739-67532758	MS.gene01558:CDS	30.0%
	TTGCACCTTCATTACATATA+TGG	+	chr2.2:67532406-67532425	None:intergenic	30.0%
!	TATATGTAATGAAGGTGCAA+CGG	-	chr2.2:67532406-67532425	MS.gene01558:intron	30.0%
!!!	CTTTGCTTTTTATCCTTCTT+AGG	-	chr2.2:67531933-67531952	MS.gene01558:CDS	30.0%
	AAATACAGTGTGGAGATAGA+TGG	-	chr2.2:67532500-67532519	MS.gene01558:intron	35.0%
	ACACGATACATATTTGACAC+CGG	+	chr2.2:67532122-67532141	None:intergenic	35.0%
	ATATATGTGACAGTGTGTGT+TGG	+	chr2.2:67532151-67532170	None:intergenic	35.0%
	ATCAGTGTCACAAACAACAA+TGG	-	chr2.2:67532623-67532642	MS.gene01558:CDS	35.0%
	CTTTCTTAAGGAAACCAAGA+GGG	+	chr2.2:67532676-67532695	None:intergenic	35.0%
	GAAGCAACAACAGATAAATC+AGG	-	chr2.2:67532476-67532495	MS.gene01558:intron	35.0%
	GTGGAATGCAAAGAAGAAAA+TGG	-	chr2.2:67532434-67532453	MS.gene01558:intron	35.0%
	TCTTTCTTAAGGAAACCAAG+AGG	+	chr2.2:67532677-67532696	None:intergenic	35.0%
	TGTGGCCATATATGTAATGA+AGG	-	chr2.2:67532398-67532417	MS.gene01558:intron	35.0%
	TTATTCTTTGCAAGAGTCCA+AGG	-	chr2.2:67532549-67532568	MS.gene01558:intron	35.0%
!	CTTTTTATCCTTCTTAGGTG+CGG	-	chr2.2:67531938-67531957	MS.gene01558:CDS	35.0%
	AAAAGCAAAGGGTGAAGACA+AGG	+	chr2.2:67531923-67531942	None:intergenic	40.0%
	AAAGCAAAGGGTGAAGACAA+GGG	+	chr2.2:67531922-67531941	None:intergenic	40.0%
	AGATTGACCGAGTTCATTGA+AGG	-	chr2.2:67532035-67532054	MS.gene01558:CDS	40.0%
	CTTCACATGTTTCATCCTCA+TGG	+	chr2.2:67532529-67532548	None:intergenic	40.0%
	GCAAGACATTCGATATGCTA+AGG	+	chr2.2:67532355-67532374	None:intergenic	40.0%
	GTACGTACCTTCAATGAACT+CGG	+	chr2.2:67532045-67532064	None:intergenic	40.0%
	TAGGTAGACGCTCTTTCTTA+AGG	+	chr2.2:67532688-67532707	None:intergenic	40.0%
	TATACTCCTCGAATTATGCG+TGG	-	chr2.2:67532095-67532114	MS.gene01558:CDS	40.0%
	TGAAGATGGAACTGAAAAGG+AGG	-	chr2.2:67532742-67532761	MS.gene01558:CDS	40.0%
	TTCAATGAACTCGGTCAATC+TGG	+	chr2.2:67532036-67532055	None:intergenic	40.0%
	TTGAGAACCTCACCACATTT+AGG	+	chr2.2:67532707-67532726	None:intergenic	40.0%
!	GGTCAATCTGGTGATGAATT+GGG	+	chr2.2:67532024-67532043	None:intergenic	40.0%
!!	AAAGAGCGTCTACCTAAATG+TGG	-	chr2.2:67532692-67532711	MS.gene01558:CDS	40.0%
	AAGCAAAGGGTGAAGACAAG+GGG	+	chr2.2:67531921-67531940	None:intergenic	45.0%
	ACCTCTGAGCAATCTTTCCT+TGG	+	chr2.2:67532569-67532588	None:intergenic	45.0%
	CCTGAACGCTTCTTTGTAGA+AGG	-	chr2.2:67531972-67531991	MS.gene01558:CDS	45.0%
	GGTGAGGTTCTCAAAGAACT+TGG	-	chr2.2:67532713-67532732	MS.gene01558:CDS	45.0%
	GTTGTTTGTGACACTGATCC+TGG	+	chr2.2:67532621-67532640	None:intergenic	45.0%
	TCCAAGGAAAGATTGCTCAG+AGG	-	chr2.2:67532565-67532584	MS.gene01558:intron	45.0%
!	CGGTCAATCTGGTGATGAAT+TGG	+	chr2.2:67532025-67532044	None:intergenic	45.0%
!!	CCTTCTACAAAGAAGCGTTC+AGG	+	chr2.2:67531975-67531994	None:intergenic	45.0%
!!	TACAAAGAAGCGTTCAGGCT+TGG	+	chr2.2:67531970-67531989	None:intergenic	45.0%
	AGCAAAGGGTGAAGACAAGG+GGG	+	chr2.2:67531920-67531939	None:intergenic	50.0%
	GCGTCTACCTAAATGTGGTG+AGG	-	chr2.2:67532697-67532716	MS.gene01558:CDS	50.0%
	GGAATAAGCCGCACCTAAGA+AGG	+	chr2.2:67531949-67531968	None:intergenic	50.0%
	GGAGATAGATGGAGACCATG+AGG	-	chr2.2:67532511-67532530	MS.gene01558:intron	50.0%
	TCCTCGAATTATGCGTGGTC+CGG	-	chr2.2:67532100-67532119	MS.gene01558:CDS	50.0%
!!	TGATCCTGGCAGCTTGTTGT+AGG	+	chr2.2:67532607-67532626	None:intergenic	50.0%
	ACCGGACCACGCATAATTCG+AGG	+	chr2.2:67532104-67532123	None:intergenic	55.0%
	CTCACCTACAACAAGCTGCC+AGG	-	chr2.2:67532600-67532619	MS.gene01558:intron	55.0%
!	AGAGGGCTAGCAGCACGAAC+TGG	+	chr2.2:67532659-67532678	None:intergenic	60.0%
!	AGGGCTAGCAGCACGAACTG+GGG	+	chr2.2:67532657-67532676	None:intergenic	60.0%
!	GAGGGCTAGCAGCACGAACT+GGG	+	chr2.2:67532658-67532677	None:intergenic	60.0%
!	GTTCGTGCTGCTAGCCCTCT+TGG	-	chr2.2:67532659-67532678	MS.gene01558:CDS	60.0%
!	TGAAGGTGCAACGGTGCGTG+TGG	-	chr2.2:67532415-67532434	MS.gene01558:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	67531900	67532769	67531900	ID=MS.gene01558
chr2.2	mRNA	67531900	67532769	67531900	ID=MS.gene01558.t1;Parent=MS.gene01558
chr2.2	exon	67532613	67532769	67532613	ID=MS.gene01558.t1.exon1;Parent=MS.gene01558.t1
chr2.2	CDS	67532613	67532769	67532613	ID=cds.MS.gene01558.t1;Parent=MS.gene01558.t1
chr2.2	exon	67531900	67532249	67531900	ID=MS.gene01558.t1.exon2;Parent=MS.gene01558.t1
chr2.2	CDS	67531900	67532249	67531900	ID=cds.MS.gene01558.t1;Parent=MS.gene01558.t1

Gene Sequence

>MS.gene01558

ATGGCAAAGTCTACAATCCCCCTTGTCTTCACCCTTTGCTTTTTATCCTTCTTAGGTGCGGCTTATTCCAAGCCTGAACGCTTCTTTGTAGAAGGTGTTGTTTACTGTGACACATGTCGCACCCAATTCATCACCAGATTGACCGAGTTCATTGAAGGTACGTACATATTTTTTAATGTGAATCTATGAAGCACATATACTCCTCGAATTATGCGTGGTCCGGTGTCAAATATGTATCGTGTCTGACACCAACACACACTGTCACATATATGTCATTTTCTTAAATCATAATCGATGTTCACATGTCATGTCGTGTCTAGTGTATAGTCTGTGCCTCGTAATACCTAAAAGTAAAATTCAGATTATTGATTTTTATTTTTTTAAAGAGTCTAACGATGAAACTCACACACATTTATTACTAAGAAGCGGAGAATTAAGAGTTCTAACTCGTGCCTTAGCATATCGAATGTCTTGCAACTTGACATAAATAATAATTTTTGTGGCCATATATGTAATGAAGGTGCAACGGTGCGTGTGGAATGCAAAGAAGAAAATGGAACATTGACATTCAACAAAGAAGCAACAACAGATAAATCAGGAAAATACAGTGTGGAGATAGATGGAGACCATGAGGATGAAACATGTGAAGTTATTCTTTGCAAGAGTCCAAGGAAAGATTGCTCAGAGGTTGACTCAGAAGCTCACCTACAACAAGCTGCCAGGATCAGTGTCACAAACAACAATGGAATTGTGTCCCCAGTTCGTGCTGCTAGCCCTCTTGGTTTCCTTAAGAAAGAGCGTCTACCTAAATGTGGTGAGGTTCTCAAAGAACTTGGAATTAATGAAGATGGAACTGAAAAGGAGGACTAG

Protein sequence

>MS.gene01558.t1

MAKSTIPLVFTLCFLSFLGAAYSKPERFFVEGVVYCDTCRTQFITRLTEFIEGATVRVECKEENGTLTFNKEATTDKSGKYSVEIDGDHEDETCEVILCKSPRKDCSEVDSEAHLQQAARISVTNNNGIVSPVRAASPLGFLKKERLPKCGEVLKELGINEDGTEKED