AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015648

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015648.t1 MTR_1g115415 98.190 442 8 0 1 442 60 501 0.0 873
MS.gene015648.t1 MTR_8g105560 83.182 440 21 2 1 440 38 424 0.0 707
MS.gene015648.t1 MTR_5g068860 53.250 400 185 1 12 409 103 502 1.94e-152 443
MS.gene015648.t1 MTR_4g070060 25.278 360 259 5 45 397 235 591 1.17e-34 136
MS.gene015648.t1 MTR_6g003960 27.164 335 238 6 65 397 263 593 4.25e-27 114
MS.gene015648.t1 MTR_2g034600 26.168 321 191 6 79 396 210 487 1.43e-26 112
MS.gene015648.t1 MTR_2g034600 22.348 264 179 5 8 267 189 430 4.94e-12 68.2
MS.gene015648.t1 MTR_2g034600 27.972 286 162 5 149 396 269 548 1.59e-26 112
MS.gene015648.t1 MTR_2g034600 22.348 264 179 5 8 267 250 491 4.55e-12 68.2
MS.gene015648.t1 MTR_2g034600 27.972 286 162 5 149 396 273 552 1.84e-26 112
MS.gene015648.t1 MTR_2g034600 22.348 264 179 5 8 267 254 495 4.57e-12 68.2
MS.gene015648.t1 MTR_4g084630 22.436 312 198 5 86 392 47 319 8.14e-26 107
MS.gene015648.t1 MTR_4g084630 25.108 231 147 3 23 252 45 250 2.97e-17 82.8
MS.gene015648.t1 MTR_5g094610 24.373 279 200 7 102 373 176 450 1.42e-19 91.3
MS.gene015648.t1 MTR_5g094610 24.627 268 186 8 22 277 194 457 5.92e-16 80.5
MS.gene015648.t1 MTR_2g049780 26.923 208 116 3 222 393 99 306 4.95e-16 79.0
MS.gene015648.t1 MTR_5g041630 27.059 255 138 7 151 397 88 302 6.43e-14 72.8
MS.gene015648.t1 MTR_2g067230 23.659 317 151 11 86 397 75 305 1.69e-13 71.6
MS.gene015648.t1 MTR_3g092710 22.819 298 184 7 99 391 235 491 1.55e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015648.t1 AT2G44020 80.587 443 85 1 1 442 65 507 0.0 733
MS.gene015648.t1 AT4G02990 53.181 393 184 0 13 405 112 504 8.61e-153 444
MS.gene015648.t1 AT4G02990 53.181 393 184 0 13 405 112 504 8.61e-153 444
MS.gene015648.t1 AT2G21710 24.716 352 261 3 77 427 278 626 8.59e-39 148
MS.gene015648.t1 AT2G21710 26.070 257 176 5 7 252 272 525 2.37e-17 85.1
MS.gene015648.t1 AT1G78930 26.488 336 240 6 65 397 249 580 1.56e-31 127
MS.gene015648.t1 AT4G38160 25.547 274 195 4 128 395 54 324 7.63e-29 116
MS.gene015648.t1 AT4G38160 24.020 204 153 2 65 266 63 266 1.09e-15 78.2
MS.gene015648.t1 AT4G38160 25.547 274 195 4 128 395 54 324 7.63e-29 116
MS.gene015648.t1 AT4G38160 24.020 204 153 2 65 266 63 266 1.09e-15 78.2
MS.gene015648.t1 AT4G38160 25.547 274 195 4 128 395 54 324 1.52e-28 115
MS.gene015648.t1 AT4G38160 23.902 205 154 2 65 267 63 267 1.59e-15 78.2
MS.gene015648.t1 AT4G38160 25.547 274 195 4 128 395 54 324 1.56e-28 116
MS.gene015648.t1 AT4G38160 23.902 205 154 2 65 267 63 267 1.42e-15 78.6
MS.gene015648.t1 AT2G21710 23.571 280 212 2 77 355 278 556 1.20e-26 112
MS.gene015648.t1 AT2G21710 26.070 257 176 5 7 252 272 525 1.93e-17 85.1
MS.gene015648.t1 AT2G21710 23.381 278 211 2 77 353 278 554 8.79e-26 110
MS.gene015648.t1 AT2G21710 26.070 257 176 5 7 252 272 525 2.01e-17 85.1
MS.gene015648.t1 AT4G14605 25.130 386 268 9 14 392 97 468 6.50e-24 104
MS.gene015648.t1 AT4G14605 25.130 386 268 9 14 392 97 468 6.50e-24 104
MS.gene015648.t1 AT5G55580 26.484 219 154 4 158 371 119 335 2.87e-19 89.7
MS.gene015648.t1 AT5G55580 25.385 260 179 7 20 267 79 335 1.46e-16 81.6
MS.gene015648.t1 AT5G55580 23.853 218 160 3 84 299 117 330 2.31e-14 74.7
MS.gene015648.t1 AT5G55580 26.484 219 154 4 158 371 225 441 1.83e-18 88.2
MS.gene015648.t1 AT5G55580 25.385 260 179 7 20 267 185 441 1.98e-16 81.6
MS.gene015648.t1 AT5G55580 23.767 223 164 3 84 304 223 441 6.22e-14 73.9
MS.gene015648.t1 AT5G54180 23.311 296 184 7 101 393 235 490 3.66e-15 77.8
MS.gene015648.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.16e-14 75.9
MS.gene015648.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.16e-14 75.9
MS.gene015648.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.16e-14 75.9
MS.gene015648.t1 AT2G34620 26.887 212 119 4 222 397 85 296 1.65e-13 71.6
MS.gene015648.t1 AT2G03050 29.317 249 151 9 158 395 38 272 4.67e-13 69.7
MS.gene015648.t1 AT2G03050 29.317 249 151 9 158 395 39 273 4.77e-13 69.7
MS.gene015648.t1 AT5G55580 25.000 172 127 2 84 253 223 394 2.12e-12 68.9
MS.gene015648.t1 AT5G55580 26.943 193 129 5 20 200 185 377 2.93e-12 68.6
MS.gene015648.t1 AT2G36000 23.506 251 169 8 158 399 79 315 4.98e-12 67.0
MS.gene015648.t1 AT2G36000 23.387 248 167 8 158 396 79 312 1.11e-11 66.2

Find 98 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAGTATCCTTTGATTT+TGG 0.073063 4.4:-1965408 MS.gene015648:CDS
GTTGAGTTTATTCAGAAATT+AGG 0.154219 4.4:-1965449 MS.gene015648:CDS
GTTGATTACTTGGTTAATTT+AGG 0.165369 4.4:-1965029 MS.gene015648:CDS
GCTCAGTATCCGCAGATTAT+TGG 0.227610 4.4:-1964867 MS.gene015648:CDS
TCCTGATGAATTGCAGGATT+TGG 0.241747 4.4:-1965507 MS.gene015648:CDS
TTGATTACTTGGTTAATTTA+GGG 0.248506 4.4:-1965028 MS.gene015648:CDS
GGATAAAATGTTCGTTGAAT+TGG 0.261081 4.4:-1964479 MS.gene015648:CDS
AGAATACTTCACTTATAGTT+TGG 0.295127 4.4:-1964544 MS.gene015648:CDS
CTCAGTATCCGCAGATTATT+GGG 0.297010 4.4:-1964866 MS.gene015648:CDS
CTTCGATTATCTCAAGGGTT+TGG 0.298946 4.4:-1965537 MS.gene015648:CDS
GTTGATTGTTTGATTAGTTT+TGG 0.307251 4.4:-1964921 MS.gene015648:CDS
CTCTTAAGCCTCTGGTATCT+AGG 0.309752 4.4:+1964507 None:intergenic
TTCGATTATCTCAAGGGTTT+GGG 0.314201 4.4:-1965536 MS.gene015648:CDS
TTGAGTTTATTCAGAAATTA+GGG 0.329984 4.4:-1965448 MS.gene015648:CDS
GATTATTGGGTTGCCTCTTA+AGG 0.337713 4.4:-1964853 MS.gene015648:CDS
CTTCAACCGTTGAAGTTATG+AGG 0.356463 4.4:-1965478 MS.gene015648:CDS
AATTATTCCTGATGAATTGC+AGG 0.358521 4.4:-1965513 MS.gene015648:CDS
CCTGTTATTAAGTTTCTTAG+AGG 0.361016 4.4:-1965251 MS.gene015648:CDS
AAACTATAAGTGAAGTATTC+TGG 0.362016 4.4:+1964546 None:intergenic
TCAAAATGTGATTATGAAAC+CGG 0.364444 4.4:-1964727 MS.gene015648:CDS
CTAATCAAACAATCAACATT+AGG 0.365413 4.4:+1964927 None:intergenic
CCTCTAAGAAACTTAATAAC+AGG 0.368936 4.4:+1965251 None:intergenic
TTAGTATTGGTGTTAATCCT+AGG 0.373470 4.4:-1965124 MS.gene015648:CDS
TCCCCTTGCTCTTAAGCCTC+TGG 0.375995 4.4:+1964499 None:intergenic
GAGATTTGAAGAGAGATTGC+AGG 0.401297 4.4:-1964436 MS.gene015648:CDS
GTCGCAGGATGTGGCTAGTA+TGG 0.403205 4.4:-1964676 MS.gene015648:CDS
GTAGCTTATCTTGTTAGTAT+TGG 0.406186 4.4:-1965137 MS.gene015648:CDS
CTCATAACTTCAACGGTTGA+AGG 0.413170 4.4:+1965479 None:intergenic
TCTGAAACTATGTCGTTTCC+TGG 0.421365 4.4:+1964351 None:intergenic
GAGAAAAGAGCTTATTTACT+TGG 0.423347 4.4:-1964975 MS.gene015648:CDS
ACCACCCTCGCAAACCCTTC+CGG 0.428125 4.4:+1964780 None:intergenic
GAAGACAATTTAGCCTTAAG+AGG 0.431995 4.4:+1964840 None:intergenic
GCAATCTCTCTTCAAATCTC+TGG 0.437449 4.4:+1964439 None:intergenic
GAATGAAGGACCTATACTTT+CGG 0.437668 4.4:+1964398 None:intergenic
TCCGGAAGGGTTTGCGAGGG+TGG 0.445823 4.4:-1964781 MS.gene015648:CDS
GGTGTTAATCCTAGGGATAT+TGG 0.451217 4.4:-1965116 MS.gene015648:CDS
TACTTCTTCAAAAGTGAGAT+GGG 0.459036 4.4:-1964597 MS.gene015648:CDS
GGAAGGGTTTGCGAGGGTGG+TGG 0.459551 4.4:-1964778 MS.gene015648:CDS
GAGGAAGGAATGTTTGCCTT+CGG 0.461475 4.4:-1964895 MS.gene015648:CDS
CGAGATGCCTACTGTTACAT+GGG 0.462699 4.4:-1965606 None:intergenic
TCCAAATCCTGCAATTCATC+AGG 0.465291 4.4:+1965506 None:intergenic
CATAGTTTCAGATGAGGAAG+AGG 0.486125 4.4:-1964340 MS.gene015648:CDS
ATCCTAGCTAGAATCTTCTT+AGG 0.491214 4.4:+1965002 None:intergenic
GGGATTTCTAGGTCTAAACT+TGG 0.492563 4.4:-1965332 MS.gene015648:CDS
ATAGGTCCTTCATTCCGTAT+TGG 0.501976 4.4:-1964390 MS.gene015648:CDS
TACCAGAGGCTTAAGAGCAA+GGG 0.502139 4.4:-1964501 MS.gene015648:CDS
CATCATCTTCGATTATCTCA+AGG 0.503798 4.4:-1965543 MS.gene015648:CDS
GGCAATACCGTCGCAGGATG+TGG 0.504137 4.4:-1964685 MS.gene015648:CDS
TTACTTCTTCAAAAGTGAGA+TGG 0.508844 4.4:-1964598 MS.gene015648:CDS
TCCTAGGGATATTGGTCCAA+TGG 0.513309 4.4:-1965108 MS.gene015648:CDS
GTTACATGGGGTGTAATTCA+AGG 0.515509 4.4:-1965593 MS.gene015648:CDS
TTAACCAAGTAATCAACAAA+AGG 0.515918 4.4:+1965035 None:intergenic
TTTGAAGCTGAAGATTGATC+CGG 0.520863 4.4:-1964799 MS.gene015648:CDS
GATGTGGAGAAAGATGATAT+TGG 0.522013 4.4:-1965224 MS.gene015648:CDS
TAGTATTGGTGTTAATCCTA+GGG 0.522188 4.4:-1965123 MS.gene015648:CDS
ACGAGATGCCTACTGTTACA+TGG 0.523419 4.4:-1965607 None:intergenic
TATATTGAAACCGAAAGTAT+AGG 0.527577 4.4:-1964408 MS.gene015648:CDS
TAAGGATACTGTGTAACCAT+TGG 0.531997 4.4:+1965092 None:intergenic
ATCATCTTCGATTATCTCAA+GGG 0.534022 4.4:-1965542 MS.gene015648:CDS
GCTCTAATTTCCCACCAATA+CGG 0.538425 4.4:+1964376 None:intergenic
GGTCCTTCATTCCGTATTGG+TGG 0.539928 4.4:-1964387 MS.gene015648:CDS
AGTTTAAACCCTAAAAGTTG+AGG 0.546911 4.4:+1965182 None:intergenic
AGCTGAAGATTGATCCGGAA+GGG 0.553058 4.4:-1964794 MS.gene015648:CDS
TTCCCACCAATACGGAATGA+AGG 0.553754 4.4:+1964384 None:intergenic
ACTCGCAGCGCAATCAATTG+AGG 0.555993 4.4:+1964642 None:intergenic
GTCCTTCATTCCGTATTGGT+GGG 0.557863 4.4:-1964386 MS.gene015648:CDS
TTCAACCGTTGAAGTTATGA+GGG 0.560167 4.4:-1965477 MS.gene015648:CDS
AGATTTGAAGAGAGATTGCA+GGG 0.561148 4.4:-1964435 MS.gene015648:CDS
ATACCAGAGGCTTAAGAGCA+AGG 0.565715 4.4:-1964502 MS.gene015648:CDS
CAAAGAGAAACTCGTTTCGC+GGG 0.570276 4.4:-1965567 MS.gene015648:CDS
GAAGATTCTAGCTAGGATGT+TGG 0.572863 4.4:-1964997 MS.gene015648:CDS
GCAAAGAGAAACTCGTTTCG+CGG 0.577091 4.4:-1965568 MS.gene015648:CDS
ACACTAGCATGCAAAACTTG+TGG 0.577739 4.4:+1965290 None:intergenic
TGGTAGGGCAATACCGTCGC+AGG 0.580954 4.4:-1964691 MS.gene015648:CDS
ACGTTCCCTCATAACTTCAA+CGG 0.581773 4.4:+1965472 None:intergenic
GAGGCCTGTAAAAGAGCTTG+TGG 0.585209 4.4:-1964574 MS.gene015648:CDS
ACACTACAGCCCAAAATCAA+AGG 0.590303 4.4:+1965398 None:intergenic
ACCAGAGGCTTAAGAGCAAG+GGG 0.605082 4.4:-1964500 MS.gene015648:CDS
GGTGGGAAATTAGAGCTACC+AGG 0.611291 4.4:-1964369 MS.gene015648:CDS
TTGATCCGGAAGGGTTTGCG+AGG 0.614047 4.4:-1964785 MS.gene015648:CDS
TGCCTAAGAAGATTCTAGCT+AGG 0.620780 4.4:-1965004 MS.gene015648:CDS
TCTTCAAAAGTGAGATGGGG+AGG 0.627180 4.4:-1964593 MS.gene015648:CDS
GTTTCTTAGAGGACTTGATG+TGG 0.627869 4.4:-1965240 MS.gene015648:CDS
GGTGGTGGAGAAGATGCCAC+AGG 0.628330 4.4:-1964763 MS.gene015648:CDS
ACCATTGGACCAATATCCCT+AGG 0.638044 4.4:+1965107 None:intergenic
ATTACACCCCATGTAACAGT+AGG 0.644138 4.4:+1965599 None:intergenic
ACTTCTTCAAAAGTGAGATG+GGG 0.645282 4.4:-1964596 MS.gene015648:CDS
TGATGAAGACTAACCACCTG+TGG 0.651476 4.4:+1964747 None:intergenic
TGATCCGGAAGGGTTTGCGA+GGG 0.652425 4.4:-1964784 MS.gene015648:CDS
TGAAATGCTATACAGAAGAA+CGG 0.652624 4.4:-1964307 MS.gene015648:CDS
AAACGACATAGTTTCAGATG+AGG 0.658299 4.4:-1964346 MS.gene015648:CDS
AAGCTGAAGATTGATCCGGA+AGG 0.664980 4.4:-1964795 MS.gene015648:CDS
ATACTAGCCACATCCTGCGA+CGG 0.678965 4.4:+1964678 None:intergenic
GGTGGAGAAGATGCCACAGG+TGG 0.679410 4.4:-1964760 MS.gene015648:CDS
GGATACTGAGCAATAACCGA+AGG 0.689064 4.4:+1964879 None:intergenic
AGAGGCAACCCAATAATCTG+CGG 0.697237 4.4:+1964858 None:intergenic
GAATTAAACCTAGATACCAG+AGG 0.721224 4.4:-1964515 MS.gene015648:CDS
GAGATGCCTACTGTTACATG+GGG 0.725465 4.4:-1965605 MS.gene015648:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAACTGAAAAAATATTGTT+GGG + chr4.4:1965083-1965102 None:intergenic 20.0%
!! TCAAACTGAAAAAATATTGT+TGG + chr4.4:1965084-1965103 None:intergenic 20.0%
!! TTGAGTTTATTCAGAAATTA+GGG - chr4.4:1964450-1964469 MS.gene015648:CDS 20.0%
!! TTGATTACTTGGTTAATTTA+GGG - chr4.4:1964870-1964889 MS.gene015648:CDS 20.0%
!!! AAAAATATCCTCAACTTTTA+GGG - chr4.4:1964708-1964727 MS.gene015648:CDS 20.0%
!!! TAGGGTATTTAGAAAAAATT+GGG - chr4.4:1964546-1964565 MS.gene015648:CDS 20.0%
!!! TTAGGGTATTTAGAAAAAAT+TGG - chr4.4:1964545-1964564 MS.gene015648:CDS 20.0%
! AGAATACTTCACTTATAGTT+TGG - chr4.4:1965354-1965373 MS.gene015648:CDS 25.0%
! CTAATCAAACAATCAACATT+AGG + chr4.4:1964974-1964993 None:intergenic 25.0%
! GTTGAGTTTATTCAGAAATT+AGG - chr4.4:1964449-1964468 MS.gene015648:CDS 25.0%
! GTTGATTACTTGGTTAATTT+AGG - chr4.4:1964869-1964888 MS.gene015648:CDS 25.0%
! TAGAAAAAATTGGGATTTCT+AGG - chr4.4:1964555-1964574 MS.gene015648:CDS 25.0%
! TCAAAATGTGATTATGAAAC+CGG - chr4.4:1965171-1965190 MS.gene015648:CDS 25.0%
! TTAACCAAGTAATCAACAAA+AGG + chr4.4:1964866-1964885 None:intergenic 25.0%
!! AAAAACATGATTCCTGTTTT+AGG - chr4.4:1964527-1964546 MS.gene015648:CDS 25.0%
!! AAAACATGATTCCTGTTTTA+GGG - chr4.4:1964528-1964547 MS.gene015648:CDS 25.0%
!! AAACTATAAGTGAAGTATTC+TGG + chr4.4:1965355-1965374 None:intergenic 25.0%
!! CAAAAATATCCTCAACTTTT+AGG - chr4.4:1964707-1964726 MS.gene015648:CDS 25.0%
!! TATATTGAAACCGAAAGTAT+AGG - chr4.4:1965490-1965509 MS.gene015648:CDS 25.0%
!! TTTTCTAAATACCCTAAAAC+AGG + chr4.4:1964542-1964561 None:intergenic 25.0%
!!! AAATCAGTATCCTTTGATTT+TGG - chr4.4:1964490-1964509 MS.gene015648:CDS 25.0%
!!! AATCAGTATCCTTTGATTTT+GGG - chr4.4:1964491-1964510 MS.gene015648:CDS 25.0%
!!! ACACAGTATCCTTATTTTTT+AGG - chr4.4:1964815-1964834 MS.gene015648:CDS 25.0%
!!! CACAGTATCCTTATTTTTTA+GGG - chr4.4:1964816-1964835 MS.gene015648:CDS 25.0%
!!! GTTGATTGTTTGATTAGTTT+TGG - chr4.4:1964977-1964996 MS.gene015648:CDS 25.0%
AATTATTCCTGATGAATTGC+AGG - chr4.4:1964385-1964404 MS.gene015648:CDS 30.0%
ACTCTCATCCCTAAAAAATA+AGG + chr4.4:1964827-1964846 None:intergenic 30.0%
AGTTTAAACCCTAAAAGTTG+AGG + chr4.4:1964719-1964738 None:intergenic 30.0%
ATCATCTTCGATTATCTCAA+GGG - chr4.4:1964356-1964375 MS.gene015648:CDS 30.0%
CCTGTTATTAAGTTTCTTAG+AGG - chr4.4:1964647-1964666 MS.gene015648:CDS 30.0%
GAGAAAAGAGCTTATTTACT+TGG - chr4.4:1964923-1964942 MS.gene015648:CDS 30.0%
GGATAAAATGTTCGTTGAAT+TGG - chr4.4:1965419-1965438 MS.gene015648:CDS 30.0%
GTAGCTTATCTTGTTAGTAT+TGG - chr4.4:1964761-1964780 MS.gene015648:CDS 30.0%
TACTTCTTCAAAAGTGAGAT+GGG - chr4.4:1965301-1965320 MS.gene015648:CDS 30.0%
TGAAATGCTATACAGAAGAA+CGG - chr4.4:1965591-1965610 MS.gene015648:CDS 30.0%
TTACTTCTTCAAAAGTGAGA+TGG - chr4.4:1965300-1965319 MS.gene015648:CDS 30.0%
! CCTCTAAGAAACTTAATAAC+AGG + chr4.4:1964650-1964669 None:intergenic 30.0%
!! AAAGCCTTTTGTTGATTACT+TGG - chr4.4:1964859-1964878 MS.gene015648:CDS 30.0%
!! TAGTATTGGTGTTAATCCTA+GGG - chr4.4:1964775-1964794 MS.gene015648:CDS 30.0%
!! TTAGTATTGGTGTTAATCCT+AGG - chr4.4:1964774-1964793 MS.gene015648:CDS 30.0%
!!! CTTTTAGGGTTTAAACTTGA+AGG - chr4.4:1964722-1964741 MS.gene015648:CDS 30.0%
!!! GTTTGATTAGTTTTGGTTTG+AGG - chr4.4:1964984-1965003 MS.gene015648:CDS 30.0%
!!! TATTTTTTAGGGATGAGAGT+TGG - chr4.4:1964827-1964846 MS.gene015648:CDS 30.0%
AAACGACATAGTTTCAGATG+AGG - chr4.4:1965552-1965571 MS.gene015648:CDS 35.0%
ACTTCTTCAAAAGTGAGATG+GGG - chr4.4:1965302-1965321 MS.gene015648:CDS 35.0%
AGATTTGAAGAGAGATTGCA+GGG - chr4.4:1965463-1965482 MS.gene015648:CDS 35.0%
ATCCTAGCTAGAATCTTCTT+AGG + chr4.4:1964899-1964918 None:intergenic 35.0%
CATCATCTTCGATTATCTCA+AGG - chr4.4:1964355-1964374 MS.gene015648:CDS 35.0%
CTACCAAGTAAAAACTCAAC+CGG + chr4.4:1965193-1965212 None:intergenic 35.0%
GAATGAAGGACCTATACTTT+CGG + chr4.4:1965503-1965522 None:intergenic 35.0%
GAATTAAACCTAGATACCAG+AGG - chr4.4:1965383-1965402 MS.gene015648:CDS 35.0%
GATGTGGAGAAAGATGATAT+TGG - chr4.4:1964674-1964693 MS.gene015648:CDS 35.0%
TAAGGATACTGTGTAACCAT+TGG + chr4.4:1964809-1964828 None:intergenic 35.0%
TTCAACCGTTGAAGTTATGA+GGG - chr4.4:1964421-1964440 MS.gene015648:CDS 35.0%
TTCGATTATCTCAAGGGTTT+GGG - chr4.4:1964362-1964381 MS.gene015648:CDS 35.0%
! AAACCGGTTGAGTTTTTACT+TGG - chr4.4:1965187-1965206 MS.gene015648:CDS 35.0%
! GGTTGAGTTTTTACTTGGTA+GGG - chr4.4:1965192-1965211 MS.gene015648:CDS 35.0%
! TTTGAAGCTGAAGATTGATC+CGG - chr4.4:1965099-1965118 MS.gene015648:CDS 35.0%
!! GAAGACAATTTAGCCTTAAG+AGG + chr4.4:1965061-1965080 None:intergenic 35.0%
!!! GATTAGTTTTGGTTTGAGGA+AGG - chr4.4:1964988-1965007 MS.gene015648:CDS 35.0%
ACACTACAGCCCAAAATCAA+AGG + chr4.4:1964503-1964522 None:intergenic 40.0%
ACACTAGCATGCAAAACTTG+TGG + chr4.4:1964611-1964630 None:intergenic 40.0%
ACGTTCCCTCATAACTTCAA+CGG + chr4.4:1964429-1964448 None:intergenic 40.0%
ATAGGTCCTTCATTCCGTAT+TGG - chr4.4:1965508-1965527 MS.gene015648:CDS 40.0%
ATTACACCCCATGTAACAGT+AGG + chr4.4:1964302-1964321 None:intergenic 40.0%
CATAGTTTCAGATGAGGAAG+AGG - chr4.4:1965558-1965577 MS.gene015648:CDS 40.0%
CTCAGTATCCGCAGATTATT+GGG - chr4.4:1965032-1965051 MS.gene015648:CDS 40.0%
CTCATAACTTCAACGGTTGA+AGG + chr4.4:1964422-1964441 None:intergenic 40.0%
CTTCAACCGTTGAAGTTATG+AGG - chr4.4:1964420-1964439 MS.gene015648:CDS 40.0%
CTTCGATTATCTCAAGGGTT+TGG - chr4.4:1964361-1964380 MS.gene015648:CDS 40.0%
GAGATTTGAAGAGAGATTGC+AGG - chr4.4:1965462-1965481 MS.gene015648:CDS 40.0%
GCAATCTCTCTTCAAATCTC+TGG + chr4.4:1965462-1965481 None:intergenic 40.0%
GCTCTAATTTCCCACCAATA+CGG + chr4.4:1965525-1965544 None:intergenic 40.0%
GGTGTTAATCCTAGGGATAT+TGG - chr4.4:1964782-1964801 MS.gene015648:CDS 40.0%
GTTACATGGGGTGTAATTCA+AGG - chr4.4:1964305-1964324 MS.gene015648:CDS 40.0%
GTTTCTTAGAGGACTTGATG+TGG - chr4.4:1964658-1964677 MS.gene015648:CDS 40.0%
TCCAAATCCTGCAATTCATC+AGG + chr4.4:1964395-1964414 None:intergenic 40.0%
TCCTGATGAATTGCAGGATT+TGG - chr4.4:1964391-1964410 MS.gene015648:CDS 40.0%
! AACTCCACAAGCTCTTTTAC+AGG + chr4.4:1965331-1965350 None:intergenic 40.0%
! CGGTTGAGTTTTTACTTGGT+AGG - chr4.4:1965191-1965210 MS.gene015648:CDS 40.0%
! GAAGATTCTAGCTAGGATGT+TGG - chr4.4:1964901-1964920 MS.gene015648:CDS 40.0%
! GATTATTGGGTTGCCTCTTA+AGG - chr4.4:1965045-1965064 MS.gene015648:CDS 40.0%
! GGGATTTCTAGGTCTAAACT+TGG - chr4.4:1964566-1964585 MS.gene015648:CDS 40.0%
! TCTGAAACTATGTCGTTTCC+TGG + chr4.4:1965550-1965569 None:intergenic 40.0%
! TGATTTTGGGCTGTAGTGTT+CGG - chr4.4:1964504-1964523 MS.gene015648:CDS 40.0%
! TGCCTAAGAAGATTCTAGCT+AGG - chr4.4:1964894-1964913 MS.gene015648:CDS 40.0%
AAGCTGAAGATTGATCCGGA+AGG - chr4.4:1965103-1965122 MS.gene015648:CDS 45.0%
ACCATTGGACCAATATCCCT+AGG + chr4.4:1964794-1964813 None:intergenic 45.0%
AGAGGCAACCCAATAATCTG+CGG + chr4.4:1965043-1965062 None:intergenic 45.0%
AGCTGAAGATTGATCCGGAA+GGG - chr4.4:1965104-1965123 MS.gene015648:CDS 45.0%
ATACCAGAGGCTTAAGAGCA+AGG - chr4.4:1965396-1965415 MS.gene015648:CDS 45.0%
CAAAGAGAAACTCGTTTCGC+GGG - chr4.4:1964331-1964350 MS.gene015648:CDS 45.0%
CTCTTAAGCCTCTGGTATCT+AGG + chr4.4:1965394-1965413 None:intergenic 45.0%
GAGGAAGGAATGTTTGCCTT+CGG - chr4.4:1965003-1965022 MS.gene015648:CDS 45.0%
GCAAAGAGAAACTCGTTTCG+CGG - chr4.4:1964330-1964349 MS.gene015648:CDS 45.0%
GCTCAGTATCCGCAGATTAT+TGG - chr4.4:1965031-1965050 MS.gene015648:CDS 45.0%
GGATACTGAGCAATAACCGA+AGG + chr4.4:1965022-1965041 None:intergenic 45.0%
TACCAGAGGCTTAAGAGCAA+GGG - chr4.4:1965397-1965416 MS.gene015648:CDS 45.0%
TCCTAGGGATATTGGTCCAA+TGG - chr4.4:1964790-1964809 MS.gene015648:CDS 45.0%
TCTTCAAAAGTGAGATGGGG+AGG - chr4.4:1965305-1965324 MS.gene015648:CDS 45.0%
TGATGAAGACTAACCACCTG+TGG + chr4.4:1965154-1965173 None:intergenic 45.0%
TTCCCACCAATACGGAATGA+AGG + chr4.4:1965517-1965536 None:intergenic 45.0%
! GTCCTTCATTCCGTATTGGT+GGG - chr4.4:1965512-1965531 MS.gene015648:CDS 45.0%
ACCAGAGGCTTAAGAGCAAG+GGG - chr4.4:1965398-1965417 MS.gene015648:CDS 50.0%
ACTCGCAGCGCAATCAATTG+AGG + chr4.4:1965259-1965278 None:intergenic 50.0%
ATACTAGCCACATCCTGCGA+CGG + chr4.4:1965223-1965242 None:intergenic 50.0%
GAGGCCTGTAAAAGAGCTTG+TGG - chr4.4:1965324-1965343 MS.gene015648:CDS 50.0%
GGTCCTTCATTCCGTATTGG+TGG - chr4.4:1965511-1965530 MS.gene015648:CDS 50.0%
GGTGGGAAATTAGAGCTACC+AGG - chr4.4:1965529-1965548 MS.gene015648:CDS 50.0%
GTCGCAGGATGTGGCTAGTA+TGG - chr4.4:1965222-1965241 MS.gene015648:CDS 55.0%
TCCCCTTGCTCTTAAGCCTC+TGG + chr4.4:1965402-1965421 None:intergenic 55.0%
TGATCCGGAAGGGTTTGCGA+GGG - chr4.4:1965114-1965133 MS.gene015648:CDS 55.0%
TTGATCCGGAAGGGTTTGCG+AGG - chr4.4:1965113-1965132 MS.gene015648:CDS 55.0%
ACCACCCTCGCAAACCCTTC+CGG + chr4.4:1965121-1965140 None:intergenic 60.0%
GGCAATACCGTCGCAGGATG+TGG - chr4.4:1965213-1965232 MS.gene015648:CDS 60.0%
GGTGGAGAAGATGCCACAGG+TGG - chr4.4:1965138-1965157 MS.gene015648:CDS 60.0%
TGGTAGGGCAATACCGTCGC+AGG - chr4.4:1965207-1965226 MS.gene015648:CDS 60.0%
!! GGTGGTGGAGAAGATGCCAC+AGG - chr4.4:1965135-1965154 MS.gene015648:CDS 60.0%
GGAAGGGTTTGCGAGGGTGG+TGG - chr4.4:1965120-1965139 MS.gene015648:CDS 65.0%
TCCGGAAGGGTTTGCGAGGG+TGG - chr4.4:1965117-1965136 MS.gene015648:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 1964296 1965624 1964296 ID=MS.gene015648
chr4.4 mRNA 1964296 1965624 1964296 ID=MS.gene015648.t1;Parent=MS.gene015648
chr4.4 exon 1964296 1965624 1964296 ID=MS.gene015648.t1.exon1;Parent=MS.gene015648.t1
chr4.4 CDS 1964296 1965624 1964296 ID=cds.MS.gene015648.t1;Parent=MS.gene015648.t1
Gene Sequence

>MS.gene015648

ATGCCTACTGTTACATGGGGTGTAATTCAAGGTCGCAAAGAGAAACTCGTTTCGCGGGTCATCATCTTCGATTATCTCAAGGGTTTGGGAATTATTCCTGATGAATTGCAGGATTTGGAGCTTCCTTCAACCGTTGAAGTTATGAGGGAACGTGTTGAGTTTATTCAGAAATTAGGGTTAACGATTGATGATATAAATCAGTATCCTTTGATTTTGGGCTGTAGTGTTCGGAAAAACATGATTCCTGTTTTAGGGTATTTAGAAAAAATTGGGATTTCTAGGTCTAAACTTGGTGAATTTATTAAGAATTATCCACAAGTTTTGCATGCTAGTGTTATTGTTGAGCTTGCTCCTGTTATTAAGTTTCTTAGAGGACTTGATGTGGAGAAAGATGATATTGGATTTGTGTTGCAAAAATATCCTCAACTTTTAGGGTTTAAACTTGAAGGTACTATGAGTACTTCAGTAGCTTATCTTGTTAGTATTGGTGTTAATCCTAGGGATATTGGTCCAATGGTTACACAGTATCCTTATTTTTTAGGGATGAGAGTTGGTACTATGATAAAGCCTTTTGTTGATTACTTGGTTAATTTAGGGTTGCCTAAGAAGATTCTAGCTAGGATGTTGGAGAAAAGAGCTTATTTACTTGGCTATGATCTTGAAGAGACTGTGAAACCTAATGTTGATTGTTTGATTAGTTTTGGTTTGAGGAAGGAATGTTTGCCTTCGGTTATTGCTCAGTATCCGCAGATTATTGGGTTGCCTCTTAAGGCTAAATTGTCTTCCCAACAATATTTTTTCAGTTTGAAGCTGAAGATTGATCCGGAAGGGTTTGCGAGGGTGGTGGAGAAGATGCCACAGGTGGTTAGTCTTCATCAAAATGTGATTATGAAACCGGTTGAGTTTTTACTTGGTAGGGCAATACCGTCGCAGGATGTGGCTAGTATGGTGATCAAGTGTCCTCAATTGATTGCGCTGCGAGTTGAGCTCATGAAGAACAGTTATTACTTCTTCAAAAGTGAGATGGGGAGGCCTGTAAAAGAGCTTGTGGAGTTTCCAGAATACTTCACTTATAGTTTGGAATCAAGAATTAAACCTAGATACCAGAGGCTTAAGAGCAAGGGGATAAAATGTTCGTTGAATTGGATGCTGAATTGTAGTGACCAGAGATTTGAAGAGAGATTGCAGGGCAATTATATTGAAACCGAAAGTATAGGTCCTTCATTCCGTATTGGTGGGAAATTAGAGCTACCAGGAAACGACATAGTTTCAGATGAGGAAGAGGAGAGTGATGATGAAATGCTATACAGAAGAACGGTTTCTCTTTAG
Protein sequence

>MS.gene015648.t1

MPTVTWGVIQGRKEKLVSRVIIFDYLKGLGIIPDELQDLELPSTVEVMRERVEFIQKLGLTIDDINQYPLILGCSVRKNMIPVLGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLRGLDVEKDDIGFVLQKYPQLLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPFVDYLVNLGLPKKILARMLEKRAYLLGYDLEETVKPNVDCLISFGLRKECLPSVIAQYPQIIGLPLKAKLSSQQYFFSLKLKIDPEGFARVVEKMPQVVSLHQNVIMKPVEFLLGRAIPSQDVASMVIKCPQLIALRVELMKNSYYFFKSEMGRPVKELVEFPEYFTYSLESRIKPRYQRLKSKGIKCSLNWMLNCSDQRFEERLQGNYIETESIGPSFRIGGKLELPGNDIVSDEEEESDDEMLYRRTVSL