AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene018579

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018579.t1 MTR_7g099570 45.719 584 231 10 9 506 6 589 1.92e-158 464
MS.gene018579.t1 MTR_1g069110 46.019 515 199 9 4 517 10 446 5.09e-140 412
MS.gene018579.t1 MTR_7g099580 90.148 203 20 0 1 203 1 203 1.04e-127 376
MS.gene018579.t1 MTR_7g099600 90.805 87 8 0 410 496 1 87 2.53e-46 157
MS.gene018579.t1 MTR_2g101310 27.133 457 275 15 43 470 137 564 3.90e-34 137
MS.gene018579.t1 MTR_8g069880 24.736 473 260 12 15 474 10 399 4.27e-34 134
MS.gene018579.t1 MTR_7g099560 30.201 298 95 8 214 510 1 186 7.80e-33 125
MS.gene018579.t1 MTR_8g062410 26.050 476 249 15 11 472 9 395 8.89e-30 122
MS.gene018579.t1 MTR_2g101310 27.717 368 226 12 43 384 137 490 1.50e-29 122
MS.gene018579.t1 MTR_7g099550 55.046 109 44 2 71 179 136 239 4.35e-29 115
MS.gene018579.t1 MTR_3g016210 23.611 504 293 14 11 497 9 437 5.06e-29 119
MS.gene018579.t1 MTR_8g073260 25.653 421 228 11 6 415 5 351 9.50e-29 119
MS.gene018579.t1 MTR_8g039620 26.104 521 275 19 7 509 9 437 1.85e-28 118
MS.gene018579.t1 MTR_2g101320 26.681 461 279 14 40 470 134 565 2.84e-28 119
MS.gene018579.t1 MTR_7g021090 25.111 450 240 15 10 445 7 373 6.21e-27 113
MS.gene018579.t1 MTR_8g066670 23.214 504 283 16 9 497 1072 1486 3.00e-25 111
MS.gene018579.t1 MTR_8g467780 24.805 512 285 13 11 501 10 442 6.24e-25 107
MS.gene018579.t1 MTR_8g068990 29.018 224 135 2 15 231 15 221 1.18e-23 104
MS.gene018579.t1 MTR_8g072510 30.137 219 142 3 15 226 13 227 4.03e-23 102
MS.gene018579.t1 MTR_7g100390 24.406 463 270 14 12 461 53 448 7.39e-21 96.3
MS.gene018579.t1 MTR_1g059930 32.759 232 132 8 12 231 12 231 8.48e-21 95.5
MS.gene018579.t1 MTR_3g105650 22.484 467 259 13 15 473 8 379 3.42e-20 93.6
MS.gene018579.t1 MTR_4g062100 23.819 508 303 19 12 510 74 506 3.89e-20 94.0
MS.gene018579.t1 MTR_1g115575 24.324 518 278 20 12 510 135 557 5.77e-20 94.0
MS.gene018579.t1 MTR_8g019290 28.571 182 109 2 321 499 6 169 1.88e-18 83.6
MS.gene018579.t1 MTR_8g019260 32.026 153 103 1 348 499 15 167 6.40e-18 82.0
MS.gene018579.t1 MTR_8g019110 32.026 153 103 1 348 499 15 167 6.40e-18 82.0
MS.gene018579.t1 MTR_2g096190 25.000 468 272 18 7 461 65 466 6.47e-18 87.4
MS.gene018579.t1 MTR_7g117620 32.447 188 114 5 47 233 119 294 9.87e-18 86.7
MS.gene018579.t1 MTR_7g117620 23.918 439 270 15 44 461 81 476 2.56e-15 79.0
MS.gene018579.t1 MTR_7g451420 29.437 231 147 6 9 231 6 228 1.15e-17 84.3
MS.gene018579.t1 MTR_8g040930 22.326 430 245 10 54 472 1 352 4.39e-17 83.6
MS.gene018579.t1 MTR_8g041240 22.326 430 245 10 54 472 1 352 4.39e-17 83.6
MS.gene018579.t1 MTR_7g087540 39.669 121 68 3 336 453 24 142 1.13e-16 78.6
MS.gene018579.t1 MTR_8g019450 30.769 195 102 4 343 505 32 225 1.97e-16 79.3
MS.gene018579.t1 MTR_8g019280 30.935 139 93 1 364 499 1 139 2.30e-16 76.6
MS.gene018579.t1 MTR_5g085500 33.333 153 96 3 81 232 8 155 2.58e-16 82.0
MS.gene018579.t1 MTR_5g085480 25.180 278 196 4 12 285 54 323 4.63e-16 81.3
MS.gene018579.t1 MTR_5g085480 25.180 278 196 4 12 285 214 483 6.32e-16 81.3
MS.gene018579.t1 MTR_0514s0010 27.232 224 122 6 314 505 13 227 6.66e-16 77.8
MS.gene018579.t1 MTR_0470s0010 30.058 173 110 5 345 509 15 184 7.78e-16 76.3
MS.gene018579.t1 MTR_8g019020 30.058 173 110 5 345 509 15 184 7.78e-16 76.3
MS.gene018579.t1 MTR_1g032100 24.831 443 255 17 12 448 60 430 1.38e-15 79.7
MS.gene018579.t1 MTR_2g033050 28.761 226 144 8 12 232 169 382 5.73e-15 77.4
MS.gene018579.t1 MTR_0470s0020 28.205 195 105 5 344 505 33 225 9.31e-15 74.7
MS.gene018579.t1 MTR_8g019050 28.205 195 105 5 344 505 33 225 9.31e-15 74.7
MS.gene018579.t1 MTR_7g100430 26.357 258 150 5 4 233 91 336 2.34e-14 76.3
MS.gene018579.t1 MTR_8g018980 29.231 195 103 5 344 505 33 225 2.83e-14 73.2
MS.gene018579.t1 MTR_8g019060 27.273 242 153 7 302 526 42 277 3.72e-13 72.0
MS.gene018579.t1 MTR_7g055630 28.235 170 116 2 348 511 16 185 1.42e-12 66.6
MS.gene018579.t1 MTR_7g087560 30.857 175 83 6 336 508 24 162 2.28e-12 65.9
MS.gene018579.t1 MTR_2g096760 30.481 187 113 7 55 231 22 201 4.44e-12 68.6
MS.gene018579.t1 MTR_8g105260 25.926 216 145 6 47 261 101 302 9.52e-12 67.8
MS.gene018579.t1 MTR_5g085810 25.641 390 226 14 42 424 299 631 9.57e-12 68.2
MS.gene018579.t1 MTR_8g019440 32.704 159 96 3 356 505 7 163 1.83e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018579.t1 AT5G51160 27.465 426 221 11 61 472 38 389 2.11e-36 140
MS.gene018579.t1 AT4G10720 25.349 501 272 17 19 510 12 419 1.05e-33 133
MS.gene018579.t1 AT5G15500 25.581 516 275 17 10 510 4 425 1.35e-33 133
MS.gene018579.t1 AT4G10720 26.253 419 225 13 19 428 12 355 8.06e-31 124
MS.gene018579.t1 AT5G50140 24.416 471 288 13 43 470 26 471 1.70e-26 113
MS.gene018579.t1 AT1G14500 23.404 517 292 14 11 511 4 432 1.15e-25 110
MS.gene018579.t1 AT1G14480 23.361 488 284 13 12 491 5 410 1.72e-24 106
MS.gene018579.t1 AT5G60070 24.706 425 251 13 47 461 111 476 1.90e-22 101
MS.gene018579.t1 AT5G54620 29.437 231 148 5 10 232 3 226 2.13e-22 100
MS.gene018579.t1 AT5G54610 32.127 221 137 5 18 232 11 224 4.59e-22 99.4
MS.gene018579.t1 AT3G13950 35.838 173 104 4 338 506 22 191 6.68e-22 94.4
MS.gene018579.t1 AT2G31820 25.870 460 263 17 12 461 193 584 1.77e-21 98.6
MS.gene018579.t1 AT4G13266 32.955 176 106 6 338 506 24 194 1.27e-19 88.2
MS.gene018579.t1 AT1G10340 23.013 478 270 15 2 471 126 513 4.63e-19 90.9
MS.gene018579.t1 AT1G10340 22.791 430 239 13 46 471 169 509 6.46e-18 87.4
MS.gene018579.t1 AT1G05640 25.054 459 269 18 12 461 157 549 1.22e-17 86.7
MS.gene018579.t1 AT3G12360 25.630 476 259 17 12 468 133 532 1.41e-17 86.3
MS.gene018579.t1 AT3G12360 25.630 476 259 17 12 468 121 520 1.51e-17 86.3
MS.gene018579.t1 AT1G14480 32.927 164 101 4 12 171 5 163 2.04e-17 84.7
MS.gene018579.t1 AT5G54710 25.000 468 262 18 15 471 143 532 3.99e-16 82.0
MS.gene018579.t1 AT5G54710 24.230 487 277 17 15 486 188 597 5.17e-16 81.6
MS.gene018579.t1 AT3G09550 25.260 384 208 14 48 427 182 490 7.72e-15 77.8
MS.gene018579.t1 AT3G09550 25.260 384 208 14 48 427 182 490 7.72e-15 77.8
MS.gene018579.t1 AT3G09550 25.260 384 208 14 48 427 189 497 8.09e-15 77.8
MS.gene018579.t1 AT1G07710 27.273 220 146 5 12 230 70 276 1.06e-14 77.4
MS.gene018579.t1 AT1G07710 27.273 220 146 5 12 230 70 276 1.06e-14 77.4
MS.gene018579.t1 AT5G02620 24.454 458 239 16 15 461 98 459 4.25e-14 75.1
MS.gene018579.t1 AT5G02620 24.454 458 239 16 15 461 98 459 4.25e-14 75.1
MS.gene018579.t1 AT5G02620 24.454 458 239 16 15 461 98 459 4.25e-14 75.1
MS.gene018579.t1 AT2G01680 22.829 403 244 12 47 448 97 433 7.03e-14 74.7
MS.gene018579.t1 AT4G03480 26.724 232 157 6 7 231 314 539 1.52e-13 73.9
MS.gene018579.t1 AT4G03480 26.724 232 157 6 7 231 311 536 1.58e-13 73.9
MS.gene018579.t1 AT5G02620 23.799 458 278 15 44 461 56 482 2.23e-13 73.2
MS.gene018579.t1 AT5G02620 23.799 458 278 15 44 461 56 482 2.23e-13 73.2
MS.gene018579.t1 AT5G54700 27.778 162 102 4 109 263 28 181 3.00e-11 65.5
MS.gene018579.t1 AT1G34050 27.570 214 139 7 33 243 140 340 7.93e-11 65.1
MS.gene018579.t1 AT4G03500 26.522 230 152 7 7 233 191 406 9.65e-11 64.7
MS.gene018579.t1 AT4G03500 26.522 230 152 7 7 233 191 406 9.65e-11 64.7

Find 89 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTAATGATTTCGGTTTC+TGG 0.183664 7.4:+10818550 None:intergenic
GTTCACTTCAGCTGCTAAAT+TGG 0.191406 7.4:+10818753 None:intergenic
TCCAACTACCTTGGTTTATT+AGG 0.226783 7.4:+10817297 None:intergenic
TGGATTTCCACTTGGGAATA+AGG 0.247146 7.4:-10817015 MS.gene018579:CDS
CTCTACAATGCATCATTAAA+TGG 0.285327 7.4:-10818922 MS.gene018579:CDS
AAATTACAAGAGTGGTGTTT+TGG 0.298168 7.4:-10816818 MS.gene018579:CDS
AAACATCACCTTAATGATTT+CGG 0.302136 7.4:+10818543 None:intergenic
TCTTTGGATCAATGTTCTTA+AGG 0.306596 7.4:+10818888 None:intergenic
AGGACGTGTGGAAGCAATTA+AGG 0.310242 7.4:-10818590 MS.gene018579:CDS
TGTGTTGACTGTGAATTCTT+TGG 0.332031 7.4:+10817392 None:intergenic
TCTTTCTTACTTCTAGTTGT+TGG 0.336507 7.4:-10816849 MS.gene018579:CDS
TCGCAAACTGGGATACGTTT+TGG 0.338912 7.4:-10816883 MS.gene018579:CDS
TGCTTCTCTGTGTGTGGTGT+CGG 0.350237 7.4:-10817048 MS.gene018579:CDS
TCAAAATGAGAGGGTCTCTT+TGG 0.353711 7.4:+10818872 None:intergenic
GCCAGAAACCGAAATCATTA+AGG 0.359546 7.4:-10818551 MS.gene018579:CDS
AGGACGTTGCCCTCTTCATT+TGG 0.363159 7.4:-10818725 MS.gene018579:CDS
ATTCTATAATAAACATGATT+AGG 0.365920 7.4:+10816906 None:intergenic
CTTCATTTGGCTTCTGCAAA+AGG 0.368217 7.4:-10818712 MS.gene018579:CDS
CTGCAGTTGTGGGTTATGTT+TGG 0.382850 7.4:-10817118 MS.gene018579:CDS
GCAGCTGAAGTGAACTTAGA+AGG 0.388437 7.4:-10818745 MS.gene018579:CDS
AAAGACAAAAGAACAATTCA+TGG 0.391103 7.4:-10817267 MS.gene018579:CDS
TGTCAGACGTGCACAAATAA+AGG 0.392121 7.4:-10818368 MS.gene018579:intron
AATATAAATTTCATAAAATC+TGG 0.393458 7.4:+10817092 None:intergenic
TTCACTTCAGCTGCTAAATT+GGG 0.395520 7.4:+10818754 None:intergenic
CTCACATACATGTGGGCACT+TGG 0.410282 7.4:-10816939 MS.gene018579:CDS
TAAGTCCACCAGGCAGTGTT+TGG 0.416873 7.4:-10817202 MS.gene018579:CDS
TTATTATAGAATTCGCAAAC+TGG 0.421423 7.4:-10816895 MS.gene018579:CDS
TTGCACATAGCCTGTTTGCT+TGG 0.442386 7.4:-10818814 MS.gene018579:CDS
GCTTCTCTGTGTGTGGTGTC+GGG 0.443308 7.4:-10817047 MS.gene018579:CDS
CTCGAAGTTTCCAAGCAAAC+AGG 0.446295 7.4:+10818804 None:intergenic
GTTTGTGTTGGTAGTGGCTG+TGG 0.452555 7.4:+10817419 None:intergenic
CTTAACACCATTGGATATGC+TGG 0.453545 7.4:-10817636 MS.gene018579:CDS
TGAAGCAGGCACTGCAGTTG+TGG 0.460591 7.4:-10817129 MS.gene018579:CDS
CTCATCAGTGGATTTCCACT+TGG 0.461862 7.4:-10817023 MS.gene018579:CDS
AATTAGCTATTCAACTGATC+AGG 0.473868 7.4:+10816744 None:intergenic
TCTTCTTGCCAAACACTGCC+TGG 0.477194 7.4:+10817194 None:intergenic
ACCTAATAAACCAAGGTAGT+TGG 0.477504 7.4:-10817298 MS.gene018579:CDS
AGCTAAAGAAGGAGCTCAAA+GGG 0.484282 7.4:-10817561 MS.gene018579:CDS
TTCATTTGGCTTCTGCAAAA+GGG 0.485852 7.4:-10818711 MS.gene018579:CDS
ACTTATGATTTCTGTGAAGC+AGG 0.486383 7.4:-10817143 MS.gene018579:CDS
GTTGGTAGTGGCTGTGGTTG+TGG 0.490786 7.4:+10817425 None:intergenic
TTCTTTGGTGATGTTTGTGT+TGG 0.490831 7.4:+10817407 None:intergenic
GGTGATGTTTGTGTTGGTAG+TGG 0.503491 7.4:+10817413 None:intergenic
TGCAATGGTGTTTCAGAGAA+GGG 0.506363 7.4:+10818832 None:intergenic
ACTTGGGAATAAGGCCATGA+TGG 0.509567 7.4:-10817006 MS.gene018579:CDS
CTTGGGAATAAGGCCATGAT+GGG 0.510342 7.4:-10817005 MS.gene018579:CDS
CAACATGGGCTTAACACCAT+TGG 0.511085 7.4:-10817645 MS.gene018579:CDS
GATCATATGTTAGCTAAAGA+AGG 0.518709 7.4:-10817572 MS.gene018579:CDS
GCGAACACACAAGTGTAGAA+TGG 0.520677 7.4:+10818501 None:intergenic
ACTTCAGTTGACGAGGAAGC+TGG 0.530077 7.4:+10817512 None:intergenic
TTTCTTTGCTTCTCTGTGTG+TGG 0.530696 7.4:-10817054 MS.gene018579:CDS
TATTATAGAATTCGCAAACT+GGG 0.531747 7.4:-10816894 MS.gene018579:CDS
ATAAATAATTTGAACAACAT+GGG 0.540801 7.4:-10817659 MS.gene018579:CDS
TATAAATAATTTGAACAACA+TGG 0.543688 7.4:-10817660 MS.gene018579:CDS
AACTGCTAAAATCCCCATCA+TGG 0.551879 7.4:+10816992 None:intergenic
ATACATGTGGGCACTTGGAT+TGG 0.556164 7.4:-10816934 MS.gene018579:CDS
TCATCAGTGGATTTCCACTT+GGG 0.562235 7.4:-10817022 MS.gene018579:CDS
GTGCAATGGTGTTTCAGAGA+AGG 0.565252 7.4:+10818831 None:intergenic
GAAGCAGGCACTGCAGTTGT+GGG 0.565583 7.4:-10817128 MS.gene018579:CDS
AATCATAAGTAGTGCATGCA+TGG 0.567093 7.4:+10817156 None:intergenic
TTTGATTCAAATTACAAGAG+TGG 0.567229 7.4:-10816826 MS.gene018579:CDS
TTGCAGAAGCCAAATGAAGA+GGG 0.567237 7.4:+10818716 None:intergenic
AACAACACAGACGCAACAGA+TGG 0.576839 7.4:-10817349 MS.gene018579:CDS
GTCCTCTCATTGCAGCAAAG+TGG 0.579978 7.4:+10818608 None:intergenic
TTATATGTCAAAGACAAAGA+AGG 0.586486 7.4:-10818412 MS.gene018579:CDS
TAGCTAAAGAAGGAGCTCAA+AGG 0.590879 7.4:-10817562 MS.gene018579:CDS
CAATCAGTAATAAGTCCACC+AGG 0.590985 7.4:-10817212 MS.gene018579:CDS
ATCCACTTTGCTGCAATGAG+AGG 0.593694 7.4:-10818610 MS.gene018579:CDS
TTCCTCTCACTAGCTGCACG+AGG 0.594845 7.4:+10818452 None:intergenic
AGCAAACAGGCTATGTGCAA+TGG 0.598670 7.4:+10818817 None:intergenic
CTCATTGCAGCAAAGTGGAT+AGG 0.598957 7.4:+10818613 None:intergenic
ATGTTTGAGACTGATCATCA+TGG 0.605579 7.4:-10818526 MS.gene018579:CDS
TCATGTTGCTCACATACATG+TGG 0.606238 7.4:-10816947 MS.gene018579:CDS
TTTGCAGAAGCCAAATGAAG+AGG 0.607614 7.4:+10818715 None:intergenic
GAAACTTTGCTCAAAATGAG+AGG 0.611802 7.4:+10818862 None:intergenic
AAACTTTGCTCAAAATGAGA+GGG 0.617414 7.4:+10818863 None:intergenic
ATCCTCGTGCAGCTAGTGAG+AGG 0.624423 7.4:-10818454 MS.gene018579:CDS
GTGGTGTCGGGTCTCATCAG+TGG 0.633735 7.4:-10817035 MS.gene018579:CDS
TCTTGCCAAACACTGCCTGG+TGG 0.647998 7.4:+10817197 None:intergenic
CATGTTGCTCACATACATGT+GGG 0.656114 7.4:-10816946 MS.gene018579:CDS
TGTTCGCTATAACCATCTAG+AGG 0.665347 7.4:-10818485 MS.gene018579:CDS
TATGTTAACTTCAGTTGACG+AGG 0.670555 7.4:+10817505 None:intergenic
ATCATGGCCTTATTCCCAAG+TGG 0.672138 7.4:+10817008 None:intergenic
AACTAGAAGTAAGAAAGACC+AGG 0.672399 7.4:+10816854 None:intergenic
TGCTGCAATGAGAGGACGTG+TGG 0.676824 7.4:-10818602 MS.gene018579:CDS
TTGGGAATAAGGCCATGATG+GGG 0.695312 7.4:-10817004 MS.gene018579:CDS
CTGTGATCCAGCATATCCAA+TGG 0.704887 7.4:+10817629 None:intergenic
ACGACATACCTAATAAACCA+AGG 0.719709 7.4:-10817305 MS.gene018579:CDS
TGGCAAGAAGATACAAAACA+CGG 0.732277 7.4:-10817182 MS.gene018579:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAATTTCATAAAATC+TGG + chr7.4:10818612-10818631 None:intergenic 10.0%
!! ATAAATAATTTGAACAACAT+GGG - chr7.4:10818042-10818061 MS.gene018579:intron 15.0%
!! ATTCTATAATAAACATGATT+AGG + chr7.4:10818798-10818817 None:intergenic 15.0%
!! GTGATTTGATATAAATAATT+TGG + chr7.4:10817416-10817435 None:intergenic 15.0%
!! TATAAATAATTTGAACAACA+TGG - chr7.4:10818041-10818060 MS.gene018579:intron 15.0%
!! TTTATAGACTTGAAAATTTA+AGG - chr7.4:10817728-10817747 MS.gene018579:intron 15.0%
!!! CTATTTTAATCTATAACAAA+CGG - chr7.4:10817438-10817457 MS.gene018579:CDS 15.0%
!!! TTCAAGTTATTTTAGAAAAA+TGG + chr7.4:10817368-10817387 None:intergenic 15.0%
!!! TTTGTTATAGATTAAAATAG+AGG + chr7.4:10817438-10817457 None:intergenic 15.0%
!! ATATAAACCATTACTGTTAT+TGG - chr7.4:10817626-10817645 MS.gene018579:CDS 20.0%
!! TATAAACCATTACTGTTATT+GGG - chr7.4:10817627-10817646 MS.gene018579:CDS 20.0%
!! TCAACATAAAACAATAGAAA+AGG - chr7.4:10817951-10817970 MS.gene018579:intron 20.0%
!! TGGTTTATATACAAGAATTA+AGG + chr7.4:10817616-10817635 None:intergenic 20.0%
! AAACATCACCTTAATGATTT+CGG + chr7.4:10817161-10817180 None:intergenic 25.0%
! AAAGACAAAAGAACAATTCA+TGG - chr7.4:10818434-10818453 MS.gene018579:CDS 25.0%
! ATGAACATGTAAGTTTCATA+CGG + chr7.4:10817932-10817951 None:intergenic 25.0%
! TATTATAGAATTCGCAAACT+GGG - chr7.4:10818807-10818826 MS.gene018579:CDS 25.0%
! TTATATGTCAAAGACAAAGA+AGG - chr7.4:10817289-10817308 MS.gene018579:CDS 25.0%
! TTATTATAGAATTCGCAAAC+TGG - chr7.4:10818806-10818825 MS.gene018579:CDS 25.0%
! TTTGATTCAAATTACAAGAG+TGG - chr7.4:10818875-10818894 MS.gene018579:CDS 25.0%
!! TATGAATGTGTTCTTTTAGA+CGG + chr7.4:10817821-10817840 None:intergenic 25.0%
!! TATTTTGTGACATTGTCATT+TGG + chr7.4:10818224-10818243 None:intergenic 25.0%
AAACTTTGCTCAAAATGAGA+GGG + chr7.4:10816841-10816860 None:intergenic 30.0%
CAATCAATACTCTTCACATT+GGG + chr7.4:10817683-10817702 None:intergenic 30.0%
CTCTACAATGCATCATTAAA+TGG - chr7.4:10816779-10816798 MS.gene018579:CDS 30.0%
GATCATATGTTAGCTAAAGA+AGG - chr7.4:10818129-10818148 MS.gene018579:intron 30.0%
TCAATCAATACTCTTCACAT+TGG + chr7.4:10817684-10817703 None:intergenic 30.0%
TCTTTGGATCAATGTTCTTA+AGG + chr7.4:10816816-10816835 None:intergenic 30.0%
TGAACATGTAAGTTTCATAC+GGG + chr7.4:10817931-10817950 None:intergenic 30.0%
! AAATTACAAGAGTGGTGTTT+TGG - chr7.4:10818883-10818902 MS.gene018579:CDS 30.0%
! ATCCAAAAACTTGCTTCAAT+AGG - chr7.4:10817047-10817066 MS.gene018579:CDS 30.0%
! GTACATTTGAAGTGATATTG+CGG + chr7.4:10817770-10817789 None:intergenic 30.0%
! TCCAAAAACTTGCTTCAATA+GGG - chr7.4:10817048-10817067 MS.gene018579:CDS 30.0%
! TCTATAAAGTCTAGTCGTTT+TGG + chr7.4:10817716-10817735 None:intergenic 30.0%
! TCTTTCTTACTTCTAGTTGT+TGG - chr7.4:10818852-10818871 MS.gene018579:CDS 30.0%
! TTTTATCAATCCAACTACCT+TGG + chr7.4:10818416-10818435 None:intergenic 30.0%
!! ATTTTCGGAGCATTTTTACT+TGG + chr7.4:10817878-10817897 None:intergenic 30.0%
!! TTTTGGATTAGGAAAAAACG+TGG - chr7.4:10818900-10818919 MS.gene018579:CDS 30.0%
AACTAGAAGTAAGAAAGACC+AGG + chr7.4:10818850-10818869 None:intergenic 35.0%
AATCATAAGTAGTGCATGCA+TGG + chr7.4:10818548-10818567 None:intergenic 35.0%
ACCTAATAAACCAAGGTAGT+TGG - chr7.4:10818403-10818422 MS.gene018579:CDS 35.0%
ACCTTAATGATTTCGGTTTC+TGG + chr7.4:10817154-10817173 None:intergenic 35.0%
ACGACATACCTAATAAACCA+AGG - chr7.4:10818396-10818415 MS.gene018579:CDS 35.0%
ACTTATGATTTCTGTGAAGC+AGG - chr7.4:10818558-10818577 MS.gene018579:CDS 35.0%
GAAACTTTGCTCAAAATGAG+AGG + chr7.4:10816842-10816861 None:intergenic 35.0%
GGATAAATTAAAGAGAGCGA+AGG + chr7.4:10817910-10817929 None:intergenic 35.0%
TATGTTAACTTCAGTTGACG+AGG + chr7.4:10818199-10818218 None:intergenic 35.0%
TCCAACTACCTTGGTTTATT+AGG + chr7.4:10818407-10818426 None:intergenic 35.0%
TGAGAACCCAATAACAGTAA+TGG + chr7.4:10817636-10817655 None:intergenic 35.0%
TGGCAAGAAGATACAAAACA+CGG - chr7.4:10818519-10818538 MS.gene018579:CDS 35.0%
TTAAGGAAAAAGCAGACTTG+AGG + chr7.4:10817599-10817618 None:intergenic 35.0%
TTCACTTCAGCTGCTAAATT+GGG + chr7.4:10816950-10816969 None:intergenic 35.0%
! ATGTTTGAGACTGATCATCA+TGG - chr7.4:10817175-10817194 MS.gene018579:CDS 35.0%
! TCCCTATTGAAGCAAGTTTT+TGG + chr7.4:10817052-10817071 None:intergenic 35.0%
! TTCATTTGGCTTCTGCAAAA+GGG - chr7.4:10816990-10817009 MS.gene018579:CDS 35.0%
!! CGAAGGTGTACATACATTTT+CGG + chr7.4:10817893-10817912 None:intergenic 35.0%
!! TGTGTTGACTGTGAATTCTT+TGG + chr7.4:10818312-10818331 None:intergenic 35.0%
!! TTCTTTGGTGATGTTTGTGT+TGG + chr7.4:10818297-10818316 None:intergenic 35.0%
!! TTGGAAACTTCGAGTTTTGT+CGG - chr7.4:10816906-10816925 MS.gene018579:CDS 35.0%
AAAGGCTCAACTTGAACACA+AGG + chr7.4:10817572-10817591 None:intergenic 40.0%
AACTGCTAAAATCCCCATCA+TGG + chr7.4:10818712-10818731 None:intergenic 40.0%
ACACAAGGTCAGATAGAATC+AGG + chr7.4:10817557-10817576 None:intergenic 40.0%
AGAATCAGGGTGAGCTAAAA+TGG + chr7.4:10817543-10817562 None:intergenic 40.0%
AGCTAAAGAAGGAGCTCAAA+GGG - chr7.4:10818140-10818159 MS.gene018579:intron 40.0%
CAATCAGTAATAAGTCCACC+AGG - chr7.4:10818489-10818508 MS.gene018579:CDS 40.0%
CACAAGGTCAGATAGAATCA+GGG + chr7.4:10817556-10817575 None:intergenic 40.0%
CATGTTGCTCACATACATGT+GGG - chr7.4:10818755-10818774 MS.gene018579:CDS 40.0%
CTTAACACCATTGGATATGC+TGG - chr7.4:10818065-10818084 MS.gene018579:intron 40.0%
GCCAGAAACCGAAATCATTA+AGG - chr7.4:10817150-10817169 MS.gene018579:CDS 40.0%
GTTCACTTCAGCTGCTAAAT+TGG + chr7.4:10816951-10816970 None:intergenic 40.0%
TAGCTAAAGAAGGAGCTCAA+AGG - chr7.4:10818139-10818158 MS.gene018579:intron 40.0%
TCAAAATGAGAGGGTCTCTT+TGG + chr7.4:10816832-10816851 None:intergenic 40.0%
TCATGTTGCTCACATACATG+TGG - chr7.4:10818754-10818773 MS.gene018579:CDS 40.0%
TGCAATGGTGTTTCAGAGAA+GGG + chr7.4:10816872-10816891 None:intergenic 40.0%
TGGATTTCCACTTGGGAATA+AGG - chr7.4:10818686-10818705 MS.gene018579:CDS 40.0%
TGTCAGACGTGCACAAATAA+AGG - chr7.4:10817333-10817352 MS.gene018579:CDS 40.0%
TGTTATTGGGTTCTCATAGC+AGG - chr7.4:10817640-10817659 MS.gene018579:CDS 40.0%
TGTTCGCTATAACCATCTAG+AGG - chr7.4:10817216-10817235 MS.gene018579:CDS 40.0%
TTGCAGAAGCCAAATGAAGA+GGG + chr7.4:10816988-10817007 None:intergenic 40.0%
TTTCTTTGCTTCTCTGTGTG+TGG - chr7.4:10818647-10818666 MS.gene018579:CDS 40.0%
TTTGCAGAAGCCAAATGAAG+AGG + chr7.4:10816989-10817008 None:intergenic 40.0%
! CTTCATTTGGCTTCTGCAAA+AGG - chr7.4:10816989-10817008 MS.gene018579:CDS 40.0%
! GGATTTTCAAAGCCTCTAGA+TGG + chr7.4:10817231-10817250 None:intergenic 40.0%
! TCATCAGTGGATTTCCACTT+GGG - chr7.4:10818679-10818698 MS.gene018579:CDS 40.0%
!! AAACTGGGATACGTTTTGGT+TGG - chr7.4:10818822-10818841 MS.gene018579:CDS 40.0%
!! CAAGAGTGGTGTTTTGGATT+AGG - chr7.4:10818889-10818908 MS.gene018579:CDS 40.0%
AACAACACAGACGCAACAGA+TGG - chr7.4:10818352-10818371 MS.gene018579:intron 45.0%
AACTCACCTAGCAAGACAGT+TGG + chr7.4:10817504-10817523 None:intergenic 45.0%
ACTTGGGAATAAGGCCATGA+TGG - chr7.4:10818695-10818714 MS.gene018579:CDS 45.0%
AGCAAACAGGCTATGTGCAA+TGG + chr7.4:10816887-10816906 None:intergenic 45.0%
AGCTAAAATGGTATGCAGGC+AGG + chr7.4:10817531-10817550 None:intergenic 45.0%
AGGACGTGTGGAAGCAATTA+AGG - chr7.4:10817111-10817130 MS.gene018579:CDS 45.0%
ATACATGTGGGCACTTGGAT+TGG - chr7.4:10818767-10818786 MS.gene018579:CDS 45.0%
ATCCACTTTGCTGCAATGAG+AGG - chr7.4:10817091-10817110 MS.gene018579:CDS 45.0%
CAACATGGGCTTAACACCAT+TGG - chr7.4:10818056-10818075 MS.gene018579:intron 45.0%
CTAGCAAGACAGTTGGAGTT+GGG + chr7.4:10817497-10817516 None:intergenic 45.0%
CTCATTGCAGCAAAGTGGAT+AGG + chr7.4:10817091-10817110 None:intergenic 45.0%
CTCGAAGTTTCCAAGCAAAC+AGG + chr7.4:10816900-10816919 None:intergenic 45.0%
CTGTGATCCAGCATATCCAA+TGG + chr7.4:10818075-10818094 None:intergenic 45.0%
CTTGGGAATAAGGCCATGAT+GGG - chr7.4:10818696-10818715 MS.gene018579:CDS 45.0%
GCAGCTGAAGTGAACTTAGA+AGG - chr7.4:10816956-10816975 MS.gene018579:CDS 45.0%
GCGAACACACAAGTGTAGAA+TGG + chr7.4:10817203-10817222 None:intergenic 45.0%
GGTGAGCTAAAATGGTATGC+AGG + chr7.4:10817535-10817554 None:intergenic 45.0%
GTGCAATGGTGTTTCAGAGA+AGG + chr7.4:10816873-10816892 None:intergenic 45.0%
TTGCACATAGCCTGTTTGCT+TGG - chr7.4:10816887-10816906 MS.gene018579:CDS 45.0%
TTGGGAATAAGGCCATGATG+GGG - chr7.4:10818697-10818716 MS.gene018579:CDS 45.0%
! CTCATCAGTGGATTTCCACT+TGG - chr7.4:10818678-10818697 MS.gene018579:CDS 45.0%
! CTGCAGTTGTGGGTTATGTT+TGG - chr7.4:10818583-10818602 MS.gene018579:CDS 45.0%
! GGTGATGTTTGTGTTGGTAG+TGG + chr7.4:10818291-10818310 None:intergenic 45.0%
! TCGCAAACTGGGATACGTTT+TGG - chr7.4:10818818-10818837 MS.gene018579:CDS 45.0%
!! ATCATGGCCTTATTCCCAAG+TGG + chr7.4:10818696-10818715 None:intergenic 45.0%
!! GTGTTCAAGTTGAGCCTTTG+AGG - chr7.4:10817573-10817592 MS.gene018579:CDS 45.0%
!!! GATACGTTTTGGTTGGTACC+TGG - chr7.4:10818829-10818848 MS.gene018579:CDS 45.0%
AAGCAGACTTGAGGCCTCAA+AGG + chr7.4:10817590-10817609 None:intergenic 50.0%
ACTTCAGTTGACGAGGAAGC+TGG + chr7.4:10818192-10818211 None:intergenic 50.0%
AGGACGTTGCCCTCTTCATT+TGG - chr7.4:10816976-10816995 MS.gene018579:CDS 50.0%
CCAACTCCAACTGTCTTGCT+AGG - chr7.4:10817495-10817514 MS.gene018579:CDS 50.0%
CCTAGCAAGACAGTTGGAGT+TGG + chr7.4:10817498-10817517 None:intergenic 50.0%
CTCACATACATGTGGGCACT+TGG - chr7.4:10818762-10818781 MS.gene018579:CDS 50.0%
GAAGTGATATTGCGGTGCGT+GGG + chr7.4:10817762-10817781 None:intergenic 50.0%
GTCCTCTCATTGCAGCAAAG+TGG + chr7.4:10817096-10817115 None:intergenic 50.0%
TAAGTCCACCAGGCAGTGTT+TGG - chr7.4:10818499-10818518 MS.gene018579:CDS 50.0%
TCTTCTTGCCAAACACTGCC+TGG + chr7.4:10818510-10818529 None:intergenic 50.0%
TGAAGTGATATTGCGGTGCG+TGG + chr7.4:10817763-10817782 None:intergenic 50.0%
TGCTTCTCTGTGTGTGGTGT+CGG - chr7.4:10818653-10818672 MS.gene018579:CDS 50.0%
!! GTTTGTGTTGGTAGTGGCTG+TGG + chr7.4:10818285-10818304 None:intergenic 50.0%
ATCCTCGTGCAGCTAGTGAG+AGG - chr7.4:10817247-10817266 MS.gene018579:CDS 55.0%
GCAAGACAGTTGGAGTTGGG+AGG + chr7.4:10817494-10817513 None:intergenic 55.0%
GCTTCTCTGTGTGTGGTGTC+GGG - chr7.4:10818654-10818673 MS.gene018579:CDS 55.0%
TCTTGCCAAACACTGCCTGG+TGG + chr7.4:10818507-10818526 None:intergenic 55.0%
TGCTGCAATGAGAGGACGTG+TGG - chr7.4:10817099-10817118 MS.gene018579:CDS 55.0%
TTCCTCTCACTAGCTGCACG+AGG + chr7.4:10817252-10817271 None:intergenic 55.0%
! GAAGCAGGCACTGCAGTTGT+GGG - chr7.4:10818573-10818592 MS.gene018579:CDS 55.0%
! TGAAGCAGGCACTGCAGTTG+TGG - chr7.4:10818572-10818591 MS.gene018579:CDS 55.0%
!! GTTGGTAGTGGCTGTGGTTG+TGG + chr7.4:10818279-10818298 None:intergenic 55.0%
! GTGGTGTCGGGTCTCATCAG+TGG - chr7.4:10818666-10818685 MS.gene018579:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 10816746 10818977 10816746 ID=MS.gene018579
chr7.4 mRNA 10816746 10818977 10816746 ID=MS.gene018579.t1;Parent=MS.gene018579
chr7.4 exon 10818369 10818977 10818369 ID=MS.gene018579.t1.exon1;Parent=MS.gene018579.t1
chr7.4 CDS 10818369 10818977 10818369 ID=cds.MS.gene018579.t1;Parent=MS.gene018579.t1
chr7.4 exon 10816746 10817723 10816746 ID=MS.gene018579.t1.exon2;Parent=MS.gene018579.t1
chr7.4 CDS 10816746 10817723 10816746 ID=cds.MS.gene018579.t1;Parent=MS.gene018579.t1
Gene Sequence

>MS.gene018579

ATGGAGATTTTACAGGATGATACAATGAGAGAGCTCTACAATGCATCATTAAATGGAAGTGTAAGTTCCTTAAGAACATTGATCCAAAGAGACCCTCTCATTTTGAGCAAAGTTTCGTTGTATCCCTTCTCTGAAACACCATTGCACATAGCCTGTTTGCTTGGAAACTTCGAGTTTTGTCGGATTCTTCTTGATATAGATCCCAATTTAGCAGCTGAAGTGAACTTAGAAGGACGTTGCCCTCTTCATTTGGCTTCTGCAAAAGGGTACACTGAGATTGTGAGAGCAATATTGTTGACAAATCCAAAAACTTGCTTCAATAGGGACAAAGATGACAAGCTTCCTATCCACTTTGCTGCAATGAGAGGACGTGTGGAAGCAATTAAGGAATTGAATAGTGTCATGCCAGAAACCGAAATCATTAAGGTGATGTTTGAGACTGATCATCATGGTTCCATTCTACACTTGTGTGTTCGCTATAACCATCTAGAGGCTTTGAAAATCCTCGTGCAGCTAGTGAGAGGAAACAATAGATTACAATTCTTATATGTCAAAGACAAAGAAGGTAACACTGTTCTGCATTTAGCTGTCAGACGTGCACAAATAAAGGTACTGCTTGCCATTTTTCTAAAATAACTTGAATGTTTTCTCAACTATAGTATTTTCTCCAAATTATTTATATCAAATCACCTCTATTTTAATCTATAACAAACGGACTTATAGTTGACTCAATCAATACATTTTACCTCCCAACTCCAACTGTCTTGCTAGGTGAGTTGTGACCTGCCTGCATACCATTTTAGCTCACCCTGATTCTATCTGACCTTGTGTTCAAGTTGAGCCTTTGAGGCCTCAAGTCTGCTTTTTCCTTAATTCTTGTATATAAACCATTACTGTTATTGGGTTCTCATAGCAGGTTGAACTTTGAGAGCGACCCAATGTGAAGAGTATTGATTGAGATATCGATCCAAAACGACTAGACTTTATAGACTTGAAAATTTAAGGAAGAACTTCCCACGCACCGCAATATCACTTCAAATGTACATGTCATAAAAAAAATGTAAATTTTATGCCGTCTAAAAGAACACATTCATACAAAACATGTCATTCAAAAGTACACAAATGTGTGCCAAGTAAAAATGCTCCGAAAATGTATGTACACCTTCGCTCTCTTTAATTTATCCCGTATGAAACTTACATGTTCATCAACATAAAACAATAGAAAAGGCTCTACTAACTTGTGTAACATTGCAGATCATTAAATACTTGCTATCACTGTCTGAAATGAGCACAGCTATAAATAATTTGAACAACATGGGCTTAACACCATTGGATATGCTGGATCACAGTTCAAGAGATTTTGATTTTATTAGTCTTACAATCGATCATATGTTAGCTAAAGAAGGAGCTCAAAGGGCTACAAATATTGTCACAACACATCTACCAGCTTCCTCGTCAACTGAAGTTAACATACACCAAATGACAATGTCACAAAATATAGTTTCTGCACAACAAGATCATATGCTCTCGCCACAACCACAGCCACTACCAACACAAACATCACCAAAGAATTCACAGTCAACACAAACATCAAACACTACTAATGAACAACACAGACGCAACAGATGGAACATGTTTGAGAACTTCTGCACGACATACCTAATAAACCAAGGTAGTTGGATTGATAAAAAGACAAAAGAACAATTCATGGTAGCAGCAACTGTAATCGCAACAATGACATTTCAATCAGTAATAAGTCCACCAGGCAGTGTTTGGCAAGAAGATACAAAACACGGTAACCATGCATGCACTACTTATGATTTCTGTGAAGCAGGCACTGCAGTTGTGGGTTATGTTTGGTCACCAGATTTTATGAAATTTATATTTTTCAACTCTGCATCTTTCTTTGCTTCTCTGTGTGTGGTGTCGGGTCTCATCAGTGGATTTCCACTTGGGAATAAGGCCATGATGGGGATTTTAGCAGTTTTAATGATAATTGCTGTCACGTTCATGTTGCTCACATACATGTGGGCACTTGGATTGGTGAGTCCTAATCATGTTTATTATAGAATTCGCAAACTGGGATACGTTTTGGTTGGTACCTGGTCTTTCTTACTTCTAGTTGTTGGTTTGATTCAAATTACAAGAGTGGTGTTTTGGATTAGGAAAAAACGTGGATTATCGACAAATGCTTTGTTGAGTCAAAATTCTCCTGATCAGTTGAATAGCTAA
Protein sequence

>MS.gene018579.t1

MEILQDDTMRELYNASLNGSVSSLRTLIQRDPLILSKVSLYPFSETPLHIACLLGNFEFCRILLDIDPNLAAEVNLEGRCPLHLASAKGYTEIVRAILLTNPKTCFNRDKDDKLPIHFAAMRGRVEAIKELNSVMPETEIIKVMFETDHHGSILHLCVRYNHLEALKILVQLVRGNNRLQFLYVKDKEGNTVLHLAVRRAQIKIIKYLLSLSEMSTAINNLNNMGLTPLDMLDHSSRDFDFISLTIDHMLAKEGAQRATNIVTTHLPASSSTEVNIHQMTMSQNIVSAQQDHMLSPQPQPLPTQTSPKNSQSTQTSNTTNEQHRRNRWNMFENFCTTYLINQGSWIDKKTKEQFMVAATVIATMTFQSVISPPGSVWQEDTKHGNHACTTYDFCEAGTAVVGYVWSPDFMKFIFFNSASFFASLCVVSGLISGFPLGNKAMMGILAVLMIIAVTFMLLTYMWALGLVSPNHVYYRIRKLGYVLVGTWSFLLLVVGLIQITRVVFWIRKKRGLSTNALLSQNSPDQLNS