Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019486.t1 | XP_013444928.1 | 94.4 | 445 | 17 | 2 | 1 | 437 | 1 | 445 | 4.20E-233 | 817 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019486.t1 | P45432 | 72.4 | 445 | 111 | 4 | 1 | 437 | 1 | 441 | 2.9e-182 | 639.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019486.t1 | A0A072TQ51 | 94.4 | 445 | 17 | 2 | 1 | 437 | 1 | 445 | 3.0e-233 | 817.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene019486 | MS.gene024064 | PPI |
| MS.gene019486 | MS.gene52605 | PPI |
| MS.gene019486 | MS.gene033505 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019486.t1 | MTR_8g432630 | 94.382 | 445 | 17 | 2 | 1 | 437 | 1 | 445 | 0.0 | 862 |
| MS.gene019486.t1 | MTR_7g076160 | 69.283 | 446 | 125 | 5 | 1 | 437 | 1 | 443 | 0.0 | 610 |
| MS.gene019486.t1 | MTR_6g025300 | 43.599 | 289 | 138 | 6 | 78 | 352 | 315 | 592 | 4.91e-62 | 214 |
| MS.gene019486.t1 | MTR_4g104080 | 26.768 | 198 | 143 | 2 | 211 | 408 | 176 | 371 | 1.64e-17 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019486.t1 | AT3G61140 | 72.360 | 445 | 111 | 4 | 1 | 437 | 1 | 441 | 0.0 | 673 |
| MS.gene019486.t1 | AT4G24820 | 25.253 | 198 | 146 | 2 | 211 | 408 | 176 | 371 | 8.93e-16 | 79.0 |
| MS.gene019486.t1 | AT4G24820 | 25.253 | 198 | 146 | 2 | 211 | 408 | 176 | 371 | 8.93e-16 | 79.0 |
| MS.gene019486.t1 | AT3G12850 | 26.875 | 320 | 165 | 12 | 38 | 335 | 11 | 283 | 1.05e-15 | 78.6 |
Find 108 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCGACAGATTTGATTTC+AGG | 0.161626 | 8.1:-69564062 | None:intergenic |
| CAAATGGGATACAATGATTT+TGG | 0.170498 | 8.1:+69562570 | MS.gene019486:CDS |
| TTAAACATGACTTCAATATT+AGG | 0.175164 | 8.1:+69564636 | MS.gene019486:CDS |
| GAGAGAATACGCAGTTGTTT+AGG | 0.234580 | 8.1:+69553871 | MS.gene019486:CDS |
| ATTTGATAGGGCAGAGTTAA+AGG | 0.247989 | 8.1:+69563163 | MS.gene019486:CDS |
| AGCTACAATTACTTCATTAT+AGG | 0.256084 | 8.1:-69563099 | None:intergenic |
| GAGAATGAGCTTAATGCTTA+TGG | 0.257163 | 8.1:+69553999 | MS.gene019486:CDS |
| GAGGGGTATGAGATGGATTT+GGG | 0.259340 | 8.1:+69553927 | MS.gene019486:CDS |
| TGAGGGGTATGAGATGGATT+TGG | 0.292623 | 8.1:+69553926 | MS.gene019486:CDS |
| TGGATTTGGGTTGGTGTGAT+GGG | 0.299034 | 8.1:+69553940 | MS.gene019486:CDS |
| TAAACATGACTTCAATATTA+GGG | 0.299578 | 8.1:+69564637 | MS.gene019486:CDS |
| GTGCACTTGCAACATTTGAT+AGG | 0.300346 | 8.1:+69563150 | MS.gene019486:CDS |
| AGAGAATACGCAGTTGTTTA+GGG | 0.301080 | 8.1:+69553872 | MS.gene019486:CDS |
| TTTCAAAGGGTTCTAGAAAC+TGG | 0.314327 | 8.1:+69564563 | MS.gene019486:CDS |
| TTATAGGTACTACCCAGTTC+TGG | 0.318469 | 8.1:-69563083 | None:intergenic |
| ATGGATTTGGGTTGGTGTGA+TGG | 0.340791 | 8.1:+69553939 | MS.gene019486:CDS |
| CGTAGCTTGGAAATTATTAT+CGG | 0.348012 | 8.1:-69562786 | None:intergenic |
| TTTATCAGTTCCCTTACTTC+GGG | 0.364996 | 8.1:-69563325 | None:intergenic |
| TCTAGAAGCAGGCCCAGAAC+TGG | 0.386575 | 8.1:+69563070 | MS.gene019486:CDS |
| ATGGGAGTGTATACTGGATA+AGG | 0.390701 | 8.1:-69564140 | None:intergenic |
| CAGCCACTATGCTTCCTGTA+TGG | 0.396394 | 8.1:+69564026 | MS.gene019486:intron |
| GCTCAAGATGTTGCAACATA+TGG | 0.403151 | 8.1:+69563119 | MS.gene019486:CDS |
| GATTAAAGAAAGCATACAAA+TGG | 0.413217 | 8.1:+69562554 | MS.gene019486:intron |
| ATACAAATGGGAGTGTATAC+TGG | 0.417410 | 8.1:-69564146 | None:intergenic |
| TAGAGTTGGTACCCGAAGTA+AGG | 0.420745 | 8.1:+69563314 | MS.gene019486:CDS |
| AGAATGAGCTTAATGCTTAT+GGG | 0.420807 | 8.1:+69554000 | MS.gene019486:CDS |
| TACTATTCTCATGGTCTACA+AGG | 0.422311 | 8.1:+69562600 | MS.gene019486:CDS |
| CTCTAAGAAATTGCGGAAGT+TGG | 0.423840 | 8.1:-69563296 | None:intergenic |
| GCATTCAAGACAACTGTTGT+TGG | 0.425392 | 8.1:+69564195 | MS.gene019486:CDS |
| TTCAAATCAACAGATACAAA+TGG | 0.429576 | 8.1:-69564159 | None:intergenic |
| GTGTAGATTTCGTCGAGCAT+TGG | 0.438562 | 8.1:-69553651 | None:intergenic |
| ATTTATCAGTTCCCTTACTT+CGG | 0.447966 | 8.1:-69563326 | None:intergenic |
| TGTAGATTTCGTCGAGCATT+GGG | 0.448276 | 8.1:-69553650 | None:intergenic |
| GATTGATGGGAGGTTGGGTG+AGG | 0.449464 | 8.1:+69553908 | MS.gene019486:CDS |
| GCAGCTCGACGTGCTTCGAT+TGG | 0.453625 | 8.1:+69553827 | MS.gene019486:CDS |
| ATCTGTTGATTTGAATATGA+TGG | 0.461634 | 8.1:+69564167 | MS.gene019486:CDS |
| TATAGGTACTACCCAGTTCT+GGG | 0.466137 | 8.1:-69563082 | None:intergenic |
| TTACTGCAGTTTCTAGAAGC+AGG | 0.470103 | 8.1:+69563059 | MS.gene019486:intron |
| TTCGTCGAGCATTGGGTTTG+AGG | 0.471588 | 8.1:-69553643 | None:intergenic |
| CTATGTTAACAAGGCAGAGC+AGG | 0.472146 | 8.1:+69562746 | MS.gene019486:CDS |
| GGATTTGGGTTGGTGTGATG+GGG | 0.475054 | 8.1:+69553941 | MS.gene019486:CDS |
| CTTCCGCAATTTCTTAGAGT+TGG | 0.479444 | 8.1:+69563300 | MS.gene019486:CDS |
| GCTTATGATGAGATTAAGAA+AGG | 0.480124 | 8.1:+69553849 | MS.gene019486:CDS |
| GAAGTTGTGATGAAGATTGA+TGG | 0.481600 | 8.1:+69553894 | MS.gene019486:CDS |
| GCTACGATGTGCCGCAGGAT+TGG | 0.487352 | 8.1:+69562803 | MS.gene019486:CDS |
| GTTGGTGTGATGGGGTTGAG+AGG | 0.492699 | 8.1:+69553949 | MS.gene019486:CDS |
| ACGGTTGGTACGACTGTTGT+CGG | 0.497826 | 8.1:-69553688 | None:intergenic |
| GATTACTGATAATCAAATAC+AGG | 0.501737 | 8.1:+69564242 | MS.gene019486:CDS |
| TCAAATCAACAGATACAAAT+GGG | 0.503406 | 8.1:-69564158 | None:intergenic |
| GATGAAGATTGATGGGAGGT+TGG | 0.508290 | 8.1:+69553902 | MS.gene019486:CDS |
| ATGAAGATTGATGGGAGGTT+GGG | 0.508768 | 8.1:+69553903 | MS.gene019486:CDS |
| TTGATGGGAGGTTGGGTGAG+GGG | 0.509103 | 8.1:+69553910 | MS.gene019486:CDS |
| GTTGTCGGCGTCGCCGCCGT+TGG | 0.509524 | 8.1:-69553673 | None:intergenic |
| TCCTGCGGCACATCGTAGCT+TGG | 0.511342 | 8.1:-69562799 | None:intergenic |
| TGGGAGTGTATACTGGATAA+GGG | 0.513868 | 8.1:-69564139 | None:intergenic |
| AAGGAATGCCACCTTTCAAA+GGG | 0.513987 | 8.1:+69564550 | MS.gene019486:CDS |
| CTTCCTGTATGGAATACCTT+GGG | 0.515326 | 8.1:+69564037 | MS.gene019486:CDS |
| GTTGGGTGAGGGGTATGAGA+TGG | 0.516428 | 8.1:+69553920 | MS.gene019486:CDS |
| TGCACTTGCAACATTTGATA+GGG | 0.517580 | 8.1:+69563151 | MS.gene019486:CDS |
| ATTAAAGAAAGCATACAAAT+GGG | 0.517865 | 8.1:+69562555 | MS.gene019486:intron |
| AAAGGAATGCCACCTTTCAA+AGG | 0.518395 | 8.1:+69564549 | MS.gene019486:CDS |
| TGGAAATTATTATCGGGTCA+AGG | 0.518981 | 8.1:-69562779 | None:intergenic |
| GTTCCCAAGGTATTCCATAC+AGG | 0.521148 | 8.1:-69564040 | None:intergenic |
| ATTGATGGGAGGTTGGGTGA+GGG | 0.521930 | 8.1:+69553909 | MS.gene019486:CDS |
| CCTGAAATCAAATCTGTCGC+TGG | 0.526883 | 8.1:+69564062 | MS.gene019486:CDS |
| TGATGGGGTTGAGAGGAAGA+TGG | 0.528170 | 8.1:+69553956 | MS.gene019486:CDS |
| AATTCTAGTCAGCATTGAGA+TGG | 0.528374 | 8.1:+69562701 | MS.gene019486:CDS |
| GCTCATGATTGCGCGACGGT+TGG | 0.532016 | 8.1:-69553703 | None:intergenic |
| TCCAAGCTACGATGTGCCGC+AGG | 0.532976 | 8.1:+69562798 | MS.gene019486:CDS |
| GTATAAGCTTGCTGCTCGAA+AGG | 0.538438 | 8.1:+69562845 | MS.gene019486:CDS |
| TTCTAGAACCCTTTGAAAGG+TGG | 0.540033 | 8.1:-69564558 | None:intergenic |
| CTAGAAGCAGGCCCAGAACT+GGG | 0.542861 | 8.1:+69563071 | MS.gene019486:CDS |
| TGATGTGGTGCAATAATCTC+GGG | 0.544295 | 8.1:-69562649 | None:intergenic |
| GGTATGAGATGGATTTGGGT+TGG | 0.544573 | 8.1:+69553931 | MS.gene019486:CDS |
| TTGATGTGGTGCAATAATCT+CGG | 0.550945 | 8.1:-69562650 | None:intergenic |
| GTAGCTTGGAAATTATTATC+GGG | 0.552194 | 8.1:-69562785 | None:intergenic |
| GGAAATTATTATCGGGTCAA+GGG | 0.556947 | 8.1:-69562778 | None:intergenic |
| GCTTCCTGTATGGAATACCT+TGG | 0.561842 | 8.1:+69564036 | MS.gene019486:CDS |
| ACTTCAATATTAGGGCATCA+AGG | 0.566142 | 8.1:+69564645 | MS.gene019486:CDS |
| ACTATTCTCATGGTCTACAA+GGG | 0.570467 | 8.1:+69562601 | MS.gene019486:CDS |
| GATTTGATTTCAGGTTCCCA+AGG | 0.575211 | 8.1:-69564053 | None:intergenic |
| CGCCGCTCATGATTGCGCGA+CGG | 0.583779 | 8.1:-69553707 | None:intergenic |
| GCTTACTCCTCTCTCTACTC+AGG | 0.588216 | 8.1:+69553747 | MS.gene019486:CDS |
| AGTTTCTAGAACCCTTTGAA+AGG | 0.589137 | 8.1:-69564561 | None:intergenic |
| CAAGATGTTGCAACATATGG+AGG | 0.589980 | 8.1:+69563122 | MS.gene019486:CDS |
| CGCAGGATTGGCAAATCTAG+AGG | 0.590488 | 8.1:+69562815 | MS.gene019486:CDS |
| AGATGGAGCAGAAGAAAGAG+AGG | 0.591119 | 8.1:+69553973 | MS.gene019486:CDS |
| AGAGTTGGTACCCGAAGTAA+GGG | 0.593120 | 8.1:+69563315 | MS.gene019486:CDS |
| ATTCTAGTCAGCATTGAGAT+GGG | 0.597136 | 8.1:+69562702 | MS.gene019486:CDS |
| AGTAAGCTTCGATGTCGAGT+TGG | 0.604462 | 8.1:-69553731 | None:intergenic |
| AAGATTTGATCTCAGCAACA+TGG | 0.606774 | 8.1:-69564612 | None:intergenic |
| CTATTCTCATGGTCTACAAG+GGG | 0.607573 | 8.1:+69562602 | MS.gene019486:CDS |
| ATGCACGACATGCAGATCAA+AGG | 0.613681 | 8.1:+69564531 | MS.gene019486:CDS |
| TGTTCGACCTGAGTAGAGAG+AGG | 0.615845 | 8.1:-69553754 | None:intergenic |
| AAGTTGTGATGAAGATTGAT+GGG | 0.618821 | 8.1:+69553895 | MS.gene019486:CDS |
| TGTCTCAAGCTATGTTAACA+AGG | 0.619773 | 8.1:+69562737 | MS.gene019486:CDS |
| AAGGGTTCTAGAAACTGGAA+AGG | 0.619982 | 8.1:+69564568 | MS.gene019486:CDS |
| ATGAATTATATGCTTTGATG+TGG | 0.624861 | 8.1:-69562664 | None:intergenic |
| AACCGTCGCGCAATCATGAG+CGG | 0.626141 | 8.1:+69553705 | MS.gene019486:CDS |
| ATTCCATACAGGAAGCATAG+TGG | 0.633208 | 8.1:-69564029 | None:intergenic |
| GTACCAACTCTAAGAAATTG+CGG | 0.634001 | 8.1:-69563303 | None:intergenic |
| TTGTGATGAAGATTGATGGG+AGG | 0.641300 | 8.1:+69553898 | MS.gene019486:CDS |
| GAAGAAGAGCAAGAACGCTA+CGG | 0.644102 | 8.1:+69553803 | MS.gene019486:CDS |
| CTCGACGAAATCTACACCAA+CGG | 0.649035 | 8.1:+69553657 | MS.gene019486:CDS |
| CTCTAGATTTGCCAATCCTG+CGG | 0.651687 | 8.1:-69562814 | None:intergenic |
| GATCGTAAAGTGTCTCAACA+TGG | 0.668297 | 8.1:-69564101 | None:intergenic |
| CAGGTCGAACAAAGATCATG+CGG | 0.684498 | 8.1:+69553766 | MS.gene019486:CDS |
| GACGAAATCTACACCAACGG+CGG | 0.698207 | 8.1:+69553660 | MS.gene019486:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 69553624 | 69564676 | 69553624 | ID=MS.gene019486 |
| chr8.1 | mRNA | 69553624 | 69564676 | 69553624 | ID=MS.gene019486.t1;Parent=MS.gene019486 |
| chr8.1 | exon | 69553624 | 69554021 | 69553624 | ID=MS.gene019486.t1.exon1;Parent=MS.gene019486.t1 |
| chr8.1 | CDS | 69553624 | 69554021 | 69553624 | ID=cds.MS.gene019486.t1;Parent=MS.gene019486.t1 |
| chr8.1 | exon | 69562566 | 69562866 | 69562566 | ID=MS.gene019486.t1.exon2;Parent=MS.gene019486.t1 |
| chr8.1 | CDS | 69562566 | 69562866 | 69562566 | ID=cds.MS.gene019486.t1;Parent=MS.gene019486.t1 |
| chr8.1 | exon | 69563068 | 69563184 | 69563068 | ID=MS.gene019486.t1.exon3;Parent=MS.gene019486.t1 |
| chr8.1 | CDS | 69563068 | 69563184 | 69563068 | ID=cds.MS.gene019486.t1;Parent=MS.gene019486.t1 |
| chr8.1 | exon | 69563277 | 69563362 | 69563277 | ID=MS.gene019486.t1.exon4;Parent=MS.gene019486.t1 |
| chr8.1 | CDS | 69563277 | 69563362 | 69563277 | ID=cds.MS.gene019486.t1;Parent=MS.gene019486.t1 |
| chr8.1 | exon | 69564029 | 69564263 | 69564029 | ID=MS.gene019486.t1.exon5;Parent=MS.gene019486.t1 |
| chr8.1 | CDS | 69564029 | 69564263 | 69564029 | ID=cds.MS.gene019486.t1;Parent=MS.gene019486.t1 |
| chr8.1 | exon | 69564500 | 69564676 | 69564500 | ID=MS.gene019486.t1.exon6;Parent=MS.gene019486.t1 |
| chr8.1 | CDS | 69564500 | 69564676 | 69564500 | ID=cds.MS.gene019486.t1;Parent=MS.gene019486.t1 |
| Gene Sequence |
| Protein sequence |
