AlfalfaGEDB | MS.gene020527

M. sativa cultivar XinJiangDaYe / MS.gene020527

KEGG: K15032
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020527.t1	XP_013470448.1	99.3	442	3	0	1	442	60	501	3.00E-138	501.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020527.t1	Q9ZT96	69.0	393	122	0	13	405	112	504	5.5e-48	193.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020527.t1	A0A072VS35	99.3	442	3	0	1	442	60	501	2.2e-138	501.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene020527.t1	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene020527	MS.gene013885	PPI
MS.gene042550	MS.gene020527	PPI
MS.gene41848	MS.gene020527	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020527.t1	MTR_1g115415	98.190	442	8	0	1	442	60	501	0.0	873
MS.gene020527.t1	MTR_8g105560	83.182	440	21	2	1	440	38	424	0.0	707
MS.gene020527.t1	MTR_5g068860	53.250	400	185	1	12	409	103	502	1.94e-152	443
MS.gene020527.t1	MTR_4g070060	25.278	360	259	5	45	397	235	591	1.17e-34	136
MS.gene020527.t1	MTR_6g003960	27.164	335	238	6	65	397	263	593	4.25e-27	114
MS.gene020527.t1	MTR_2g034600	26.168	321	191	6	79	396	210	487	1.43e-26	112
MS.gene020527.t1	MTR_2g034600	22.348	264	179	5	8	267	189	430	4.94e-12	68.2
MS.gene020527.t1	MTR_2g034600	27.972	286	162	5	149	396	269	548	1.59e-26	112
MS.gene020527.t1	MTR_2g034600	22.348	264	179	5	8	267	250	491	4.55e-12	68.2
MS.gene020527.t1	MTR_2g034600	27.972	286	162	5	149	396	273	552	1.84e-26	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020527.t1	AT2G44020	80.587	443	85	1	1	442	65	507	0.0	733
MS.gene020527.t1	AT4G02990	53.181	393	184	0	13	405	112	504	8.61e-153	444
MS.gene020527.t1	AT4G02990	53.181	393	184	0	13	405	112	504	8.61e-153	444
MS.gene020527.t1	AT2G21710	24.716	352	261	3	77	427	278	626	8.59e-39	148
MS.gene020527.t1	AT2G21710	26.070	257	176	5	7	252	272	525	2.37e-17	85.1
MS.gene020527.t1	AT1G78930	26.488	336	240	6	65	397	249	580	1.56e-31	127
MS.gene020527.t1	AT4G38160	25.547	274	195	4	128	395	54	324	7.63e-29	116
MS.gene020527.t1	AT4G38160	24.020	204	153	2	65	266	63	266	1.09e-15	78.2
MS.gene020527.t1	AT4G38160	25.547	274	195	4	128	395	54	324	7.63e-29	116
MS.gene020527.t1	AT4G38160	24.020	204	153	2	65	266	63	266	1.09e-15	78.2

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Find 98 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGACATAGTTTCAGATG+AGG	0.658299	4.1:+1543262	MS.gene020527:CDS
AAACTATAAGTGAAGTATTC+TGG	0.362016	4.1:-1543062	None:intergenic
AAATCAGTATCCTTTGATTT+TGG	0.073063	4.1:+1542200	MS.gene020527:CDS
AAGCTTAAGATTGATCCGGA+AGG	0.621599	4.1:+1542813	MS.gene020527:CDS
AATTATTCCTGATGAATTGC+AGG	0.358521	4.1:+1542095	MS.gene020527:CDS
ACACTACAGCCCAAAATCAA+AGG	0.590303	4.1:-1542210	None:intergenic
ACACTAGCATGCAAAACTTG+TGG	0.577739	4.1:-1542318	None:intergenic
ACCACCCTCGCAAACCCTTC+CGG	0.428125	4.1:-1542828	None:intergenic
ACCAGAGGCTTAAGAGCAAG+GGG	0.605082	4.1:+1543108	MS.gene020527:CDS
ACCATTGGACCAATATCCCT+AGG	0.638044	4.1:-1542501	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTATTCCTGATGAATTGC+AGG	+	chr4.1:1542095-1542114	MS.gene020527:CDS	30.0%
ACTCTCATCCCTAAAAAATA+AGG	-	chr4.1:1542537-1542556	None:intergenic	30.0%
AGTTTAAACCCTAAAAGTTG+AGG	-	chr4.1:1542429-1542448	None:intergenic	30.0%
ATCATCTTCGATTATCTCAA+GGG	+	chr4.1:1542066-1542085	MS.gene020527:CDS	30.0%
CCTGTTATTAAGTTTCTTAG+AGG	+	chr4.1:1542357-1542376	MS.gene020527:CDS	30.0%
GAGAAAAGAGCTTATTTACT+TGG	+	chr4.1:1542633-1542652	MS.gene020527:CDS	30.0%
GGATAAAATGTTCGTTGAAT+TGG	+	chr4.1:1543129-1543148	MS.gene020527:CDS	30.0%
GTAGCTTATCTTGTTAGTAT+TGG	+	chr4.1:1542471-1542490	MS.gene020527:CDS	30.0%
TACTTCTTCAAAAGTGAGAT+GGG	+	chr4.1:1543011-1543030	MS.gene020527:CDS	30.0%
TGAAATGCTATACAGAAGAA+CGG	+	chr4.1:1543301-1543320	MS.gene020527:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	1542006	1543334	1542006	ID=MS.gene020527
chr4.1	mRNA	1542006	1543334	1542006	ID=MS.gene020527.t1;Parent=MS.gene020527
chr4.1	exon	1542006	1543334	1542006	ID=MS.gene020527.t1.exon1;Parent=MS.gene020527.t1
chr4.1	CDS	1542006	1543334	1542006	ID=cds.MS.gene020527.t1;Parent=MS.gene020527.t1

Gene Sequence

>MS.gene020527

ATGCCTACTGTTACATGGGGTGTAATTCAAGGTCGCAAAGAGAAACTCGTTTCGCGGGTCATCATCTTCGATTATCTCAAGGGTTTGGGAATTATTCCTGATGAATTGCAGGATTTGGAGCTTCCTTCAACCGTTGAAGTTATGAGGGAACGTGTTGAGTTTATTCAGAAATTAGGGTTAACGATTGATGATATAAATCAGTATCCTTTGATTTTGGGCTGTAGTGTTCGGAAAAACATGATTCCTGTTTTAGGGTATTTAGAAAAAATTGGGATTTCTAGGTCTAAACTTGGTGAATTTATTAAGAATTATCCACAAGTTTTGCATGCTAGTGTTATTGTTGAGCTTGCTCCTGTTATTAAGTTTCTTAGAGGACTTGATGTGGAGAAAGATGATATTGGATTTGTGTTGCAAAAATATCCTCAACTTTTAGGGTTTAAACTTGAAGGTACTATGAGTACTTCAGTAGCTTATCTTGTTAGTATTGGTGTTAATCCTAGGGATATTGGTCCAATGGTTACACAGTATCCTTATTTTTTAGGGATGAGAGTTGGTACTATGATAAAGCCTTTTGTTGATTACTTGGTTAATTTAGGGTTGCCTAAGAAGATTCTAGCTAGGATGTTGGAGAAAAGAGCTTATTTACTTGGCTATGATCTTGAAGAGACTGTGAAACCTAATGTTGATTGTTTGATTAGTTTTGGTTTGAGGAAGGAATGTTTGCCTTCGGTTATTGCTCAGTATCCGCAGATTATTGGGTTGCCTCTTAAGGCTAAATTGTCTTCCCAACAATATTTTTTCAGTTTGAAGCTTAAGATTGATCCGGAAGGGTTTGCGAGGGTGGTGGAGAAGATGCCACAGGTGGTTAGTCTTCATCAAAATGTGATTATGAAACCGGTTGAGTTTTTACTTGGTAGGGCAATACCGTCGCAGGATGTGGCTAGTATGGTGATCAAGTGTCCTCAATTGATTGCGCTGCGAGTTGAGCTCATGAAGAACAGTTATTACTTCTTCAAAAGTGAGATGGGGAGGCCTGTAAAAGAGCTTGTGGAGTTTCCAGAATACTTCACTTATAGTTTGGAATCAAGAATTAAACCTAGATACCAGAGGCTTAAGAGCAAGGGGATAAAATGTTCGTTGAATTGGATGCTGAATTGTAGTGACCAGAGATTTGAAGAGAGATTGCAGGGCAATTATATTGAAACCGAAAGTATAGGTCCTTCATTCCGTATTGGTGGGAAATTAGAGCTACCAGGAAACGACATAGTTTCAGATGAGGAAGAGGAGAGTGATGATGAAATGCTATACAGAAGAACGGTTTCTCTTTAG

Protein sequence

>MS.gene020527.t1

MPTVTWGVIQGRKEKLVSRVIIFDYLKGLGIIPDELQDLELPSTVEVMRERVEFIQKLGLTIDDINQYPLILGCSVRKNMIPVLGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLRGLDVEKDDIGFVLQKYPQLLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPFVDYLVNLGLPKKILARMLEKRAYLLGYDLEETVKPNVDCLISFGLRKECLPSVIAQYPQIIGLPLKAKLSSQQYFFSLKLKIDPEGFARVVEKMPQVVSLHQNVIMKPVEFLLGRAIPSQDVASMVIKCPQLIALRVELMKNSYYFFKSEMGRPVKELVEFPEYFTYSLESRIKPRYQRLKSKGIKCSLNWMLNCSDQRFEERLQGNYIETESIGPSFRIGGKLELPGNDIVSDEEEESDDEMLYRRTVSL