AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020527

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020527.t1 MTR_1g115415 98.190 442 8 0 1 442 60 501 0.0 873
MS.gene020527.t1 MTR_8g105560 83.182 440 21 2 1 440 38 424 0.0 707
MS.gene020527.t1 MTR_5g068860 53.250 400 185 1 12 409 103 502 1.94e-152 443
MS.gene020527.t1 MTR_4g070060 25.278 360 259 5 45 397 235 591 1.17e-34 136
MS.gene020527.t1 MTR_6g003960 27.164 335 238 6 65 397 263 593 4.25e-27 114
MS.gene020527.t1 MTR_2g034600 26.168 321 191 6 79 396 210 487 1.43e-26 112
MS.gene020527.t1 MTR_2g034600 22.348 264 179 5 8 267 189 430 4.94e-12 68.2
MS.gene020527.t1 MTR_2g034600 27.972 286 162 5 149 396 269 548 1.59e-26 112
MS.gene020527.t1 MTR_2g034600 22.348 264 179 5 8 267 250 491 4.55e-12 68.2
MS.gene020527.t1 MTR_2g034600 27.972 286 162 5 149 396 273 552 1.84e-26 112
MS.gene020527.t1 MTR_2g034600 22.348 264 179 5 8 267 254 495 4.57e-12 68.2
MS.gene020527.t1 MTR_4g084630 22.436 312 198 5 86 392 47 319 8.14e-26 107
MS.gene020527.t1 MTR_4g084630 25.108 231 147 3 23 252 45 250 2.97e-17 82.8
MS.gene020527.t1 MTR_5g094610 24.373 279 200 7 102 373 176 450 1.42e-19 91.3
MS.gene020527.t1 MTR_5g094610 24.627 268 186 8 22 277 194 457 5.92e-16 80.5
MS.gene020527.t1 MTR_2g049780 26.923 208 116 3 222 393 99 306 4.95e-16 79.0
MS.gene020527.t1 MTR_5g041630 27.059 255 138 7 151 397 88 302 6.43e-14 72.8
MS.gene020527.t1 MTR_2g067230 23.659 317 151 11 86 397 75 305 1.69e-13 71.6
MS.gene020527.t1 MTR_3g092710 22.819 298 184 7 99 391 235 491 1.55e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020527.t1 AT2G44020 80.587 443 85 1 1 442 65 507 0.0 733
MS.gene020527.t1 AT4G02990 53.181 393 184 0 13 405 112 504 8.61e-153 444
MS.gene020527.t1 AT4G02990 53.181 393 184 0 13 405 112 504 8.61e-153 444
MS.gene020527.t1 AT2G21710 24.716 352 261 3 77 427 278 626 8.59e-39 148
MS.gene020527.t1 AT2G21710 26.070 257 176 5 7 252 272 525 2.37e-17 85.1
MS.gene020527.t1 AT1G78930 26.488 336 240 6 65 397 249 580 1.56e-31 127
MS.gene020527.t1 AT4G38160 25.547 274 195 4 128 395 54 324 7.63e-29 116
MS.gene020527.t1 AT4G38160 24.020 204 153 2 65 266 63 266 1.09e-15 78.2
MS.gene020527.t1 AT4G38160 25.547 274 195 4 128 395 54 324 7.63e-29 116
MS.gene020527.t1 AT4G38160 24.020 204 153 2 65 266 63 266 1.09e-15 78.2
MS.gene020527.t1 AT4G38160 25.547 274 195 4 128 395 54 324 1.52e-28 115
MS.gene020527.t1 AT4G38160 23.902 205 154 2 65 267 63 267 1.59e-15 78.2
MS.gene020527.t1 AT4G38160 25.547 274 195 4 128 395 54 324 1.56e-28 116
MS.gene020527.t1 AT4G38160 23.902 205 154 2 65 267 63 267 1.42e-15 78.6
MS.gene020527.t1 AT2G21710 23.571 280 212 2 77 355 278 556 1.20e-26 112
MS.gene020527.t1 AT2G21710 26.070 257 176 5 7 252 272 525 1.93e-17 85.1
MS.gene020527.t1 AT2G21710 23.381 278 211 2 77 353 278 554 8.79e-26 110
MS.gene020527.t1 AT2G21710 26.070 257 176 5 7 252 272 525 2.01e-17 85.1
MS.gene020527.t1 AT4G14605 25.130 386 268 9 14 392 97 468 6.50e-24 104
MS.gene020527.t1 AT4G14605 25.130 386 268 9 14 392 97 468 6.50e-24 104
MS.gene020527.t1 AT5G55580 26.484 219 154 4 158 371 119 335 2.87e-19 89.7
MS.gene020527.t1 AT5G55580 25.385 260 179 7 20 267 79 335 1.46e-16 81.6
MS.gene020527.t1 AT5G55580 23.853 218 160 3 84 299 117 330 2.31e-14 74.7
MS.gene020527.t1 AT5G55580 26.484 219 154 4 158 371 225 441 1.83e-18 88.2
MS.gene020527.t1 AT5G55580 25.385 260 179 7 20 267 185 441 1.98e-16 81.6
MS.gene020527.t1 AT5G55580 23.767 223 164 3 84 304 223 441 6.22e-14 73.9
MS.gene020527.t1 AT5G54180 23.311 296 184 7 101 393 235 490 3.66e-15 77.8
MS.gene020527.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.16e-14 75.9
MS.gene020527.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.16e-14 75.9
MS.gene020527.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.16e-14 75.9
MS.gene020527.t1 AT2G34620 26.887 212 119 4 222 397 85 296 1.65e-13 71.6
MS.gene020527.t1 AT2G03050 29.317 249 151 9 158 395 38 272 4.67e-13 69.7
MS.gene020527.t1 AT2G03050 29.317 249 151 9 158 395 39 273 4.77e-13 69.7
MS.gene020527.t1 AT5G55580 25.000 172 127 2 84 253 223 394 2.12e-12 68.9
MS.gene020527.t1 AT5G55580 26.943 193 129 5 20 200 185 377 2.93e-12 68.6
MS.gene020527.t1 AT2G36000 23.506 251 169 8 158 399 79 315 4.98e-12 67.0
MS.gene020527.t1 AT2G36000 23.387 248 167 8 158 396 79 312 1.11e-11 66.2

Find 98 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAGTATCCTTTGATTT+TGG 0.073063 4.1:+1542200 MS.gene020527:CDS
GTTGAGTTTATTCAGAAATT+AGG 0.154219 4.1:+1542159 MS.gene020527:CDS
GTTGATTACTTGGTTAATTT+AGG 0.165369 4.1:+1542579 MS.gene020527:CDS
GCTCAGTATCCGCAGATTAT+TGG 0.227610 4.1:+1542741 MS.gene020527:CDS
TCCTGATGAATTGCAGGATT+TGG 0.241747 4.1:+1542101 MS.gene020527:CDS
TTGATTACTTGGTTAATTTA+GGG 0.248506 4.1:+1542580 MS.gene020527:CDS
GGATAAAATGTTCGTTGAAT+TGG 0.261081 4.1:+1543129 MS.gene020527:CDS
AGAATACTTCACTTATAGTT+TGG 0.295127 4.1:+1543064 MS.gene020527:CDS
CTCAGTATCCGCAGATTATT+GGG 0.297010 4.1:+1542742 MS.gene020527:CDS
CTTCGATTATCTCAAGGGTT+TGG 0.298946 4.1:+1542071 MS.gene020527:CDS
GTTGATTGTTTGATTAGTTT+TGG 0.307251 4.1:+1542687 MS.gene020527:CDS
CTCTTAAGCCTCTGGTATCT+AGG 0.309752 4.1:-1543101 None:intergenic
TTCGATTATCTCAAGGGTTT+GGG 0.314201 4.1:+1542072 MS.gene020527:CDS
TTGAGTTTATTCAGAAATTA+GGG 0.329984 4.1:+1542160 MS.gene020527:CDS
GATTATTGGGTTGCCTCTTA+AGG 0.337713 4.1:+1542755 MS.gene020527:CDS
CTTCAACCGTTGAAGTTATG+AGG 0.356463 4.1:+1542130 MS.gene020527:CDS
AATTATTCCTGATGAATTGC+AGG 0.358521 4.1:+1542095 MS.gene020527:CDS
CCTGTTATTAAGTTTCTTAG+AGG 0.361016 4.1:+1542357 MS.gene020527:CDS
AAACTATAAGTGAAGTATTC+TGG 0.362016 4.1:-1543062 None:intergenic
TCAAAATGTGATTATGAAAC+CGG 0.364444 4.1:+1542881 MS.gene020527:CDS
CTAATCAAACAATCAACATT+AGG 0.365413 4.1:-1542681 None:intergenic
CCTCTAAGAAACTTAATAAC+AGG 0.368936 4.1:-1542357 None:intergenic
TTAGTATTGGTGTTAATCCT+AGG 0.373470 4.1:+1542484 MS.gene020527:CDS
TCCCCTTGCTCTTAAGCCTC+TGG 0.375995 4.1:-1543109 None:intergenic
GAGATTTGAAGAGAGATTGC+AGG 0.401297 4.1:+1543172 MS.gene020527:CDS
GTCGCAGGATGTGGCTAGTA+TGG 0.403205 4.1:+1542932 MS.gene020527:CDS
GTAGCTTATCTTGTTAGTAT+TGG 0.406186 4.1:+1542471 MS.gene020527:CDS
CTCATAACTTCAACGGTTGA+AGG 0.413170 4.1:-1542129 None:intergenic
TCTGAAACTATGTCGTTTCC+TGG 0.421365 4.1:-1543257 None:intergenic
GAGAAAAGAGCTTATTTACT+TGG 0.423347 4.1:+1542633 MS.gene020527:CDS
ACCACCCTCGCAAACCCTTC+CGG 0.428125 4.1:-1542828 None:intergenic
GAAGACAATTTAGCCTTAAG+AGG 0.431995 4.1:-1542768 None:intergenic
GCAATCTCTCTTCAAATCTC+TGG 0.437449 4.1:-1543169 None:intergenic
GAATGAAGGACCTATACTTT+CGG 0.437668 4.1:-1543210 None:intergenic
TCCGGAAGGGTTTGCGAGGG+TGG 0.445823 4.1:+1542827 MS.gene020527:CDS
GGTGTTAATCCTAGGGATAT+TGG 0.451217 4.1:+1542492 MS.gene020527:CDS
TACTTCTTCAAAAGTGAGAT+GGG 0.459036 4.1:+1543011 MS.gene020527:CDS
GGAAGGGTTTGCGAGGGTGG+TGG 0.459551 4.1:+1542830 MS.gene020527:CDS
GAGGAAGGAATGTTTGCCTT+CGG 0.461475 4.1:+1542713 MS.gene020527:CDS
CGAGATGCCTACTGTTACAT+GGG 0.462699 4.1:+1542002 None:intergenic
TCCAAATCCTGCAATTCATC+AGG 0.465291 4.1:-1542102 None:intergenic
CATAGTTTCAGATGAGGAAG+AGG 0.486125 4.1:+1543268 MS.gene020527:CDS
ATCCTAGCTAGAATCTTCTT+AGG 0.491214 4.1:-1542606 None:intergenic
GGGATTTCTAGGTCTAAACT+TGG 0.492563 4.1:+1542276 MS.gene020527:CDS
ATAGGTCCTTCATTCCGTAT+TGG 0.501976 4.1:+1543218 MS.gene020527:CDS
TACCAGAGGCTTAAGAGCAA+GGG 0.502139 4.1:+1543107 MS.gene020527:CDS
CATCATCTTCGATTATCTCA+AGG 0.503798 4.1:+1542065 MS.gene020527:CDS
GGCAATACCGTCGCAGGATG+TGG 0.504137 4.1:+1542923 MS.gene020527:CDS
TTTGAAGCTTAAGATTGATC+CGG 0.506836 4.1:+1542809 MS.gene020527:CDS
TTACTTCTTCAAAAGTGAGA+TGG 0.508844 4.1:+1543010 MS.gene020527:CDS
TCCTAGGGATATTGGTCCAA+TGG 0.513309 4.1:+1542500 MS.gene020527:CDS
GTTACATGGGGTGTAATTCA+AGG 0.515509 4.1:+1542015 MS.gene020527:CDS
TTAACCAAGTAATCAACAAA+AGG 0.515918 4.1:-1542573 None:intergenic
GATGTGGAGAAAGATGATAT+TGG 0.522013 4.1:+1542384 MS.gene020527:CDS
TAGTATTGGTGTTAATCCTA+GGG 0.522188 4.1:+1542485 MS.gene020527:CDS
ACGAGATGCCTACTGTTACA+TGG 0.523419 4.1:+1542001 None:intergenic
TATATTGAAACCGAAAGTAT+AGG 0.527577 4.1:+1543200 MS.gene020527:CDS
TAAGGATACTGTGTAACCAT+TGG 0.531997 4.1:-1542516 None:intergenic
ATCATCTTCGATTATCTCAA+GGG 0.534022 4.1:+1542066 MS.gene020527:CDS
GCTCTAATTTCCCACCAATA+CGG 0.538425 4.1:-1543232 None:intergenic
GGTCCTTCATTCCGTATTGG+TGG 0.539928 4.1:+1543221 MS.gene020527:CDS
AGTTTAAACCCTAAAAGTTG+AGG 0.546911 4.1:-1542426 None:intergenic
TTCCCACCAATACGGAATGA+AGG 0.553754 4.1:-1543224 None:intergenic
ACTCGCAGCGCAATCAATTG+AGG 0.555993 4.1:-1542966 None:intergenic
AGCTTAAGATTGATCCGGAA+GGG 0.556415 4.1:+1542814 MS.gene020527:CDS
GTCCTTCATTCCGTATTGGT+GGG 0.557863 4.1:+1543222 MS.gene020527:CDS
TTCAACCGTTGAAGTTATGA+GGG 0.560167 4.1:+1542131 MS.gene020527:CDS
AGATTTGAAGAGAGATTGCA+GGG 0.561148 4.1:+1543173 MS.gene020527:CDS
ATACCAGAGGCTTAAGAGCA+AGG 0.565715 4.1:+1543106 MS.gene020527:CDS
CAAAGAGAAACTCGTTTCGC+GGG 0.570276 4.1:+1542041 MS.gene020527:CDS
GAAGATTCTAGCTAGGATGT+TGG 0.572863 4.1:+1542611 MS.gene020527:CDS
GCAAAGAGAAACTCGTTTCG+CGG 0.577091 4.1:+1542040 MS.gene020527:CDS
ACACTAGCATGCAAAACTTG+TGG 0.577739 4.1:-1542318 None:intergenic
TGGTAGGGCAATACCGTCGC+AGG 0.580954 4.1:+1542917 MS.gene020527:CDS
ACGTTCCCTCATAACTTCAA+CGG 0.581773 4.1:-1542136 None:intergenic
GAGGCCTGTAAAAGAGCTTG+TGG 0.585209 4.1:+1543034 MS.gene020527:CDS
ACACTACAGCCCAAAATCAA+AGG 0.590303 4.1:-1542210 None:intergenic
ACCAGAGGCTTAAGAGCAAG+GGG 0.605082 4.1:+1543108 MS.gene020527:CDS
GGTGGGAAATTAGAGCTACC+AGG 0.611291 4.1:+1543239 MS.gene020527:CDS
TTGATCCGGAAGGGTTTGCG+AGG 0.614047 4.1:+1542823 MS.gene020527:CDS
TGCCTAAGAAGATTCTAGCT+AGG 0.620780 4.1:+1542604 MS.gene020527:CDS
AAGCTTAAGATTGATCCGGA+AGG 0.621599 4.1:+1542813 MS.gene020527:CDS
TCTTCAAAAGTGAGATGGGG+AGG 0.627180 4.1:+1543015 MS.gene020527:CDS
GTTTCTTAGAGGACTTGATG+TGG 0.627869 4.1:+1542368 MS.gene020527:CDS
GGTGGTGGAGAAGATGCCAC+AGG 0.628330 4.1:+1542845 MS.gene020527:CDS
ACCATTGGACCAATATCCCT+AGG 0.638044 4.1:-1542501 None:intergenic
ATTACACCCCATGTAACAGT+AGG 0.644138 4.1:-1542009 None:intergenic
ACTTCTTCAAAAGTGAGATG+GGG 0.645282 4.1:+1543012 MS.gene020527:CDS
TGATGAAGACTAACCACCTG+TGG 0.651476 4.1:-1542861 None:intergenic
TGATCCGGAAGGGTTTGCGA+GGG 0.652425 4.1:+1542824 MS.gene020527:CDS
TGAAATGCTATACAGAAGAA+CGG 0.652624 4.1:+1543301 MS.gene020527:CDS
AAACGACATAGTTTCAGATG+AGG 0.658299 4.1:+1543262 MS.gene020527:CDS
ATACTAGCCACATCCTGCGA+CGG 0.678965 4.1:-1542930 None:intergenic
GGTGGAGAAGATGCCACAGG+TGG 0.679410 4.1:+1542848 MS.gene020527:CDS
GGATACTGAGCAATAACCGA+AGG 0.689064 4.1:-1542729 None:intergenic
AGAGGCAACCCAATAATCTG+CGG 0.697237 4.1:-1542750 None:intergenic
GAATTAAACCTAGATACCAG+AGG 0.721224 4.1:+1543093 MS.gene020527:CDS
GAGATGCCTACTGTTACATG+GGG 0.725465 4.1:+1542003 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAACTGAAAAAATATTGTT+GGG - chr4.1:1542793-1542812 None:intergenic 20.0%
!! TCAAACTGAAAAAATATTGT+TGG - chr4.1:1542794-1542813 None:intergenic 20.0%
!! TTGAGTTTATTCAGAAATTA+GGG + chr4.1:1542160-1542179 MS.gene020527:CDS 20.0%
!! TTGATTACTTGGTTAATTTA+GGG + chr4.1:1542580-1542599 MS.gene020527:CDS 20.0%
!!! AAAAATATCCTCAACTTTTA+GGG + chr4.1:1542418-1542437 MS.gene020527:CDS 20.0%
!!! TAGGGTATTTAGAAAAAATT+GGG + chr4.1:1542256-1542275 MS.gene020527:CDS 20.0%
!!! TTAGGGTATTTAGAAAAAAT+TGG + chr4.1:1542255-1542274 MS.gene020527:CDS 20.0%
! AGAATACTTCACTTATAGTT+TGG + chr4.1:1543064-1543083 MS.gene020527:CDS 25.0%
! CTAATCAAACAATCAACATT+AGG - chr4.1:1542684-1542703 None:intergenic 25.0%
! GTTGAGTTTATTCAGAAATT+AGG + chr4.1:1542159-1542178 MS.gene020527:CDS 25.0%
! GTTGATTACTTGGTTAATTT+AGG + chr4.1:1542579-1542598 MS.gene020527:CDS 25.0%
! TAGAAAAAATTGGGATTTCT+AGG + chr4.1:1542265-1542284 MS.gene020527:CDS 25.0%
! TCAAAATGTGATTATGAAAC+CGG + chr4.1:1542881-1542900 MS.gene020527:CDS 25.0%
! TTAACCAAGTAATCAACAAA+AGG - chr4.1:1542576-1542595 None:intergenic 25.0%
!! AAAAACATGATTCCTGTTTT+AGG + chr4.1:1542237-1542256 MS.gene020527:CDS 25.0%
!! AAAACATGATTCCTGTTTTA+GGG + chr4.1:1542238-1542257 MS.gene020527:CDS 25.0%
!! AAACTATAAGTGAAGTATTC+TGG - chr4.1:1543065-1543084 None:intergenic 25.0%
!! CAAAAATATCCTCAACTTTT+AGG + chr4.1:1542417-1542436 MS.gene020527:CDS 25.0%
!! TATATTGAAACCGAAAGTAT+AGG + chr4.1:1543200-1543219 MS.gene020527:CDS 25.0%
!! TTTTCTAAATACCCTAAAAC+AGG - chr4.1:1542252-1542271 None:intergenic 25.0%
!!! AAATCAGTATCCTTTGATTT+TGG + chr4.1:1542200-1542219 MS.gene020527:CDS 25.0%
!!! AATCAGTATCCTTTGATTTT+GGG + chr4.1:1542201-1542220 MS.gene020527:CDS 25.0%
!!! ACACAGTATCCTTATTTTTT+AGG + chr4.1:1542525-1542544 MS.gene020527:CDS 25.0%
!!! CACAGTATCCTTATTTTTTA+GGG + chr4.1:1542526-1542545 MS.gene020527:CDS 25.0%
!!! GTTGATTGTTTGATTAGTTT+TGG + chr4.1:1542687-1542706 MS.gene020527:CDS 25.0%
AATTATTCCTGATGAATTGC+AGG + chr4.1:1542095-1542114 MS.gene020527:CDS 30.0%
ACTCTCATCCCTAAAAAATA+AGG - chr4.1:1542537-1542556 None:intergenic 30.0%
AGTTTAAACCCTAAAAGTTG+AGG - chr4.1:1542429-1542448 None:intergenic 30.0%
ATCATCTTCGATTATCTCAA+GGG + chr4.1:1542066-1542085 MS.gene020527:CDS 30.0%
CCTGTTATTAAGTTTCTTAG+AGG + chr4.1:1542357-1542376 MS.gene020527:CDS 30.0%
GAGAAAAGAGCTTATTTACT+TGG + chr4.1:1542633-1542652 MS.gene020527:CDS 30.0%
GGATAAAATGTTCGTTGAAT+TGG + chr4.1:1543129-1543148 MS.gene020527:CDS 30.0%
GTAGCTTATCTTGTTAGTAT+TGG + chr4.1:1542471-1542490 MS.gene020527:CDS 30.0%
TACTTCTTCAAAAGTGAGAT+GGG + chr4.1:1543011-1543030 MS.gene020527:CDS 30.0%
TGAAATGCTATACAGAAGAA+CGG + chr4.1:1543301-1543320 MS.gene020527:CDS 30.0%
TTACTTCTTCAAAAGTGAGA+TGG + chr4.1:1543010-1543029 MS.gene020527:CDS 30.0%
! CCTCTAAGAAACTTAATAAC+AGG - chr4.1:1542360-1542379 None:intergenic 30.0%
! TTTGAAGCTTAAGATTGATC+CGG + chr4.1:1542809-1542828 MS.gene020527:CDS 30.0%
!! AAAGCCTTTTGTTGATTACT+TGG + chr4.1:1542569-1542588 MS.gene020527:CDS 30.0%
!! TAGTATTGGTGTTAATCCTA+GGG + chr4.1:1542485-1542504 MS.gene020527:CDS 30.0%
!! TTAGTATTGGTGTTAATCCT+AGG + chr4.1:1542484-1542503 MS.gene020527:CDS 30.0%
!!! CTTTTAGGGTTTAAACTTGA+AGG + chr4.1:1542432-1542451 MS.gene020527:CDS 30.0%
!!! GTTTGATTAGTTTTGGTTTG+AGG + chr4.1:1542694-1542713 MS.gene020527:CDS 30.0%
!!! TATTTTTTAGGGATGAGAGT+TGG + chr4.1:1542537-1542556 MS.gene020527:CDS 30.0%
AAACGACATAGTTTCAGATG+AGG + chr4.1:1543262-1543281 MS.gene020527:CDS 35.0%
ACTTCTTCAAAAGTGAGATG+GGG + chr4.1:1543012-1543031 MS.gene020527:CDS 35.0%
AGATTTGAAGAGAGATTGCA+GGG + chr4.1:1543173-1543192 MS.gene020527:CDS 35.0%
ATCCTAGCTAGAATCTTCTT+AGG - chr4.1:1542609-1542628 None:intergenic 35.0%
CATCATCTTCGATTATCTCA+AGG + chr4.1:1542065-1542084 MS.gene020527:CDS 35.0%
CTACCAAGTAAAAACTCAAC+CGG - chr4.1:1542903-1542922 None:intergenic 35.0%
GAATGAAGGACCTATACTTT+CGG - chr4.1:1543213-1543232 None:intergenic 35.0%
GAATTAAACCTAGATACCAG+AGG + chr4.1:1543093-1543112 MS.gene020527:CDS 35.0%
GATGTGGAGAAAGATGATAT+TGG + chr4.1:1542384-1542403 MS.gene020527:CDS 35.0%
TAAGGATACTGTGTAACCAT+TGG - chr4.1:1542519-1542538 None:intergenic 35.0%
TTCAACCGTTGAAGTTATGA+GGG + chr4.1:1542131-1542150 MS.gene020527:CDS 35.0%
TTCGATTATCTCAAGGGTTT+GGG + chr4.1:1542072-1542091 MS.gene020527:CDS 35.0%
! AAACCGGTTGAGTTTTTACT+TGG + chr4.1:1542897-1542916 MS.gene020527:CDS 35.0%
! GGTTGAGTTTTTACTTGGTA+GGG + chr4.1:1542902-1542921 MS.gene020527:CDS 35.0%
!! GAAGACAATTTAGCCTTAAG+AGG - chr4.1:1542771-1542790 None:intergenic 35.0%
!!! GATTAGTTTTGGTTTGAGGA+AGG + chr4.1:1542698-1542717 MS.gene020527:CDS 35.0%
AAGCTTAAGATTGATCCGGA+AGG + chr4.1:1542813-1542832 MS.gene020527:CDS 40.0%
ACACTACAGCCCAAAATCAA+AGG - chr4.1:1542213-1542232 None:intergenic 40.0%
ACACTAGCATGCAAAACTTG+TGG - chr4.1:1542321-1542340 None:intergenic 40.0%
ACGTTCCCTCATAACTTCAA+CGG - chr4.1:1542139-1542158 None:intergenic 40.0%
AGCTTAAGATTGATCCGGAA+GGG + chr4.1:1542814-1542833 MS.gene020527:CDS 40.0%
ATAGGTCCTTCATTCCGTAT+TGG + chr4.1:1543218-1543237 MS.gene020527:CDS 40.0%
ATTACACCCCATGTAACAGT+AGG - chr4.1:1542012-1542031 None:intergenic 40.0%
CATAGTTTCAGATGAGGAAG+AGG + chr4.1:1543268-1543287 MS.gene020527:CDS 40.0%
CTCAGTATCCGCAGATTATT+GGG + chr4.1:1542742-1542761 MS.gene020527:CDS 40.0%
CTCATAACTTCAACGGTTGA+AGG - chr4.1:1542132-1542151 None:intergenic 40.0%
CTTCAACCGTTGAAGTTATG+AGG + chr4.1:1542130-1542149 MS.gene020527:CDS 40.0%
CTTCGATTATCTCAAGGGTT+TGG + chr4.1:1542071-1542090 MS.gene020527:CDS 40.0%
GAGATTTGAAGAGAGATTGC+AGG + chr4.1:1543172-1543191 MS.gene020527:CDS 40.0%
GCAATCTCTCTTCAAATCTC+TGG - chr4.1:1543172-1543191 None:intergenic 40.0%
GCTCTAATTTCCCACCAATA+CGG - chr4.1:1543235-1543254 None:intergenic 40.0%
GGTGTTAATCCTAGGGATAT+TGG + chr4.1:1542492-1542511 MS.gene020527:CDS 40.0%
GTTACATGGGGTGTAATTCA+AGG + chr4.1:1542015-1542034 MS.gene020527:CDS 40.0%
GTTTCTTAGAGGACTTGATG+TGG + chr4.1:1542368-1542387 MS.gene020527:CDS 40.0%
TCCAAATCCTGCAATTCATC+AGG - chr4.1:1542105-1542124 None:intergenic 40.0%
TCCTGATGAATTGCAGGATT+TGG + chr4.1:1542101-1542120 MS.gene020527:CDS 40.0%
! AACTCCACAAGCTCTTTTAC+AGG - chr4.1:1543041-1543060 None:intergenic 40.0%
! CGGTTGAGTTTTTACTTGGT+AGG + chr4.1:1542901-1542920 MS.gene020527:CDS 40.0%
! GAAGATTCTAGCTAGGATGT+TGG + chr4.1:1542611-1542630 MS.gene020527:CDS 40.0%
! GATTATTGGGTTGCCTCTTA+AGG + chr4.1:1542755-1542774 MS.gene020527:CDS 40.0%
! GGGATTTCTAGGTCTAAACT+TGG + chr4.1:1542276-1542295 MS.gene020527:CDS 40.0%
! TCTGAAACTATGTCGTTTCC+TGG - chr4.1:1543260-1543279 None:intergenic 40.0%
! TGATTTTGGGCTGTAGTGTT+CGG + chr4.1:1542214-1542233 MS.gene020527:CDS 40.0%
! TGCCTAAGAAGATTCTAGCT+AGG + chr4.1:1542604-1542623 MS.gene020527:CDS 40.0%
ACCATTGGACCAATATCCCT+AGG - chr4.1:1542504-1542523 None:intergenic 45.0%
AGAGGCAACCCAATAATCTG+CGG - chr4.1:1542753-1542772 None:intergenic 45.0%
ATACCAGAGGCTTAAGAGCA+AGG + chr4.1:1543106-1543125 MS.gene020527:CDS 45.0%
CAAAGAGAAACTCGTTTCGC+GGG + chr4.1:1542041-1542060 MS.gene020527:CDS 45.0%
CTCTTAAGCCTCTGGTATCT+AGG - chr4.1:1543104-1543123 None:intergenic 45.0%
GAGGAAGGAATGTTTGCCTT+CGG + chr4.1:1542713-1542732 MS.gene020527:CDS 45.0%
GCAAAGAGAAACTCGTTTCG+CGG + chr4.1:1542040-1542059 MS.gene020527:CDS 45.0%
GCTCAGTATCCGCAGATTAT+TGG + chr4.1:1542741-1542760 MS.gene020527:CDS 45.0%
GGATACTGAGCAATAACCGA+AGG - chr4.1:1542732-1542751 None:intergenic 45.0%
TACCAGAGGCTTAAGAGCAA+GGG + chr4.1:1543107-1543126 MS.gene020527:CDS 45.0%
TCCTAGGGATATTGGTCCAA+TGG + chr4.1:1542500-1542519 MS.gene020527:CDS 45.0%
TCTTCAAAAGTGAGATGGGG+AGG + chr4.1:1543015-1543034 MS.gene020527:CDS 45.0%
TGATGAAGACTAACCACCTG+TGG - chr4.1:1542864-1542883 None:intergenic 45.0%
TTCCCACCAATACGGAATGA+AGG - chr4.1:1543227-1543246 None:intergenic 45.0%
! GTCCTTCATTCCGTATTGGT+GGG + chr4.1:1543222-1543241 MS.gene020527:CDS 45.0%
ACCAGAGGCTTAAGAGCAAG+GGG + chr4.1:1543108-1543127 MS.gene020527:CDS 50.0%
ACTCGCAGCGCAATCAATTG+AGG - chr4.1:1542969-1542988 None:intergenic 50.0%
ATACTAGCCACATCCTGCGA+CGG - chr4.1:1542933-1542952 None:intergenic 50.0%
GAGGCCTGTAAAAGAGCTTG+TGG + chr4.1:1543034-1543053 MS.gene020527:CDS 50.0%
GGTCCTTCATTCCGTATTGG+TGG + chr4.1:1543221-1543240 MS.gene020527:CDS 50.0%
GGTGGGAAATTAGAGCTACC+AGG + chr4.1:1543239-1543258 MS.gene020527:CDS 50.0%
GTCGCAGGATGTGGCTAGTA+TGG + chr4.1:1542932-1542951 MS.gene020527:CDS 55.0%
TCCCCTTGCTCTTAAGCCTC+TGG - chr4.1:1543112-1543131 None:intergenic 55.0%
TGATCCGGAAGGGTTTGCGA+GGG + chr4.1:1542824-1542843 MS.gene020527:CDS 55.0%
TTGATCCGGAAGGGTTTGCG+AGG + chr4.1:1542823-1542842 MS.gene020527:CDS 55.0%
ACCACCCTCGCAAACCCTTC+CGG - chr4.1:1542831-1542850 None:intergenic 60.0%
GGCAATACCGTCGCAGGATG+TGG + chr4.1:1542923-1542942 MS.gene020527:CDS 60.0%
GGTGGAGAAGATGCCACAGG+TGG + chr4.1:1542848-1542867 MS.gene020527:CDS 60.0%
TGGTAGGGCAATACCGTCGC+AGG + chr4.1:1542917-1542936 MS.gene020527:CDS 60.0%
!! GGTGGTGGAGAAGATGCCAC+AGG + chr4.1:1542845-1542864 MS.gene020527:CDS 60.0%
GGAAGGGTTTGCGAGGGTGG+TGG + chr4.1:1542830-1542849 MS.gene020527:CDS 65.0%
TCCGGAAGGGTTTGCGAGGG+TGG + chr4.1:1542827-1542846 MS.gene020527:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 1542006 1543334 1542006 ID=MS.gene020527
chr4.1 mRNA 1542006 1543334 1542006 ID=MS.gene020527.t1;Parent=MS.gene020527
chr4.1 exon 1542006 1543334 1542006 ID=MS.gene020527.t1.exon1;Parent=MS.gene020527.t1
chr4.1 CDS 1542006 1543334 1542006 ID=cds.MS.gene020527.t1;Parent=MS.gene020527.t1
Gene Sequence

>MS.gene020527

ATGCCTACTGTTACATGGGGTGTAATTCAAGGTCGCAAAGAGAAACTCGTTTCGCGGGTCATCATCTTCGATTATCTCAAGGGTTTGGGAATTATTCCTGATGAATTGCAGGATTTGGAGCTTCCTTCAACCGTTGAAGTTATGAGGGAACGTGTTGAGTTTATTCAGAAATTAGGGTTAACGATTGATGATATAAATCAGTATCCTTTGATTTTGGGCTGTAGTGTTCGGAAAAACATGATTCCTGTTTTAGGGTATTTAGAAAAAATTGGGATTTCTAGGTCTAAACTTGGTGAATTTATTAAGAATTATCCACAAGTTTTGCATGCTAGTGTTATTGTTGAGCTTGCTCCTGTTATTAAGTTTCTTAGAGGACTTGATGTGGAGAAAGATGATATTGGATTTGTGTTGCAAAAATATCCTCAACTTTTAGGGTTTAAACTTGAAGGTACTATGAGTACTTCAGTAGCTTATCTTGTTAGTATTGGTGTTAATCCTAGGGATATTGGTCCAATGGTTACACAGTATCCTTATTTTTTAGGGATGAGAGTTGGTACTATGATAAAGCCTTTTGTTGATTACTTGGTTAATTTAGGGTTGCCTAAGAAGATTCTAGCTAGGATGTTGGAGAAAAGAGCTTATTTACTTGGCTATGATCTTGAAGAGACTGTGAAACCTAATGTTGATTGTTTGATTAGTTTTGGTTTGAGGAAGGAATGTTTGCCTTCGGTTATTGCTCAGTATCCGCAGATTATTGGGTTGCCTCTTAAGGCTAAATTGTCTTCCCAACAATATTTTTTCAGTTTGAAGCTTAAGATTGATCCGGAAGGGTTTGCGAGGGTGGTGGAGAAGATGCCACAGGTGGTTAGTCTTCATCAAAATGTGATTATGAAACCGGTTGAGTTTTTACTTGGTAGGGCAATACCGTCGCAGGATGTGGCTAGTATGGTGATCAAGTGTCCTCAATTGATTGCGCTGCGAGTTGAGCTCATGAAGAACAGTTATTACTTCTTCAAAAGTGAGATGGGGAGGCCTGTAAAAGAGCTTGTGGAGTTTCCAGAATACTTCACTTATAGTTTGGAATCAAGAATTAAACCTAGATACCAGAGGCTTAAGAGCAAGGGGATAAAATGTTCGTTGAATTGGATGCTGAATTGTAGTGACCAGAGATTTGAAGAGAGATTGCAGGGCAATTATATTGAAACCGAAAGTATAGGTCCTTCATTCCGTATTGGTGGGAAATTAGAGCTACCAGGAAACGACATAGTTTCAGATGAGGAAGAGGAGAGTGATGATGAAATGCTATACAGAAGAACGGTTTCTCTTTAG
Protein sequence

>MS.gene020527.t1

MPTVTWGVIQGRKEKLVSRVIIFDYLKGLGIIPDELQDLELPSTVEVMRERVEFIQKLGLTIDDINQYPLILGCSVRKNMIPVLGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLRGLDVEKDDIGFVLQKYPQLLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPFVDYLVNLGLPKKILARMLEKRAYLLGYDLEETVKPNVDCLISFGLRKECLPSVIAQYPQIIGLPLKAKLSSQQYFFSLKLKIDPEGFARVVEKMPQVVSLHQNVIMKPVEFLLGRAIPSQDVASMVIKCPQLIALRVELMKNSYYFFKSEMGRPVKELVEFPEYFTYSLESRIKPRYQRLKSKGIKCSLNWMLNCSDQRFEERLQGNYIETESIGPSFRIGGKLELPGNDIVSDEEEESDDEMLYRRTVSL