Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020858.t1 | XP_003617922.1 | 99.8 | 473 | 1 | 0 | 1 | 473 | 1 | 473 | 1.10E-268 | 935.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020858.t1 | Q9ZW34 | 82.6 | 472 | 82 | 0 | 1 | 472 | 1 | 472 | 1.3e-228 | 793.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020858.t1 | G7K4W1 | 99.8 | 473 | 1 | 0 | 1 | 473 | 1 | 473 | 8.3e-269 | 935.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene007304 | MS.gene020858 | PPI |
| MS.gene045798 | MS.gene020858 | PPI |
| MS.gene78187 | MS.gene020858 | PPI |
| MS.gene83234 | MS.gene020858 | PPI |
| MS.gene020556 | MS.gene020858 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020858.t1 | MTR_5g096970 | 99.789 | 473 | 1 | 0 | 1 | 473 | 1 | 473 | 0.0 | 972 |
| MS.gene020858.t1 | MTR_7g103620 | 59.361 | 438 | 167 | 7 | 45 | 472 | 2 | 438 | 1.76e-180 | 512 |
| MS.gene020858.t1 | MTR_2g056190 | 58.605 | 430 | 175 | 3 | 44 | 472 | 62 | 489 | 1.87e-177 | 506 |
| MS.gene020858.t1 | MTR_6g060570 | 56.979 | 437 | 159 | 8 | 47 | 472 | 5 | 423 | 2.69e-165 | 473 |
| MS.gene020858.t1 | MTR_7g103620 | 58.713 | 373 | 145 | 5 | 109 | 472 | 16 | 388 | 1.16e-151 | 437 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020858.t1 | AT2G29560 | 82.627 | 472 | 82 | 0 | 1 | 472 | 1 | 472 | 0.0 | 822 |
| MS.gene020858.t1 | AT2G36530 | 58.998 | 439 | 169 | 7 | 45 | 473 | 2 | 439 | 3.30e-179 | 509 |
| MS.gene020858.t1 | AT1G74030 | 58.503 | 441 | 173 | 5 | 40 | 472 | 37 | 475 | 1.57e-177 | 506 |
| MS.gene020858.t1 | AT2G36530 | 58.134 | 418 | 164 | 7 | 66 | 473 | 12 | 428 | 3.94e-167 | 478 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACGGGGTCGGAGGTTT+TGG | 0.248202 | 5.4:-76127882 | None:intergenic |
| TGAAACCTATCATCATTTAA+AGG | 0.252430 | 5.4:+76132957 | MS.gene020858:CDS |
| GCAGGCAAAGGAAGCGCATT+GGG | 0.283596 | 5.4:+76137144 | MS.gene020858:CDS |
| CCTTGACAAAACAGAGAAAA+AGG | 0.305149 | 5.4:+76130590 | MS.gene020858:CDS |
| GGATCTTCTATTGATACAAT+TGG | 0.313466 | 5.4:-76135858 | None:intergenic |
| TTGTAATGCTCTTCTTCTTA+AGG | 0.320244 | 5.4:+76136607 | MS.gene020858:CDS |
| GGCTCTGACGGCGGCATTGA+CGG | 0.326530 | 5.4:-76127861 | None:intergenic |
| TGACGGCGTCTTCGAGTTTA+CGG | 0.335239 | 5.4:-76127844 | None:intergenic |
| AATCACCTTTAAATGATGAT+AGG | 0.353600 | 5.4:-76132962 | None:intergenic |
| TCAAATCTGCTTGCTCCAAT+TGG | 0.357446 | 5.4:-76132913 | None:intergenic |
| TTCATAGCTGATTTATCTGT+TGG | 0.364955 | 5.4:+76137208 | MS.gene020858:CDS |
| ATCCATTTGACAAGGAAGAT+TGG | 0.376004 | 5.4:+76135877 | MS.gene020858:CDS |
| TCCATTGCTGCATGTAAAGC+TGG | 0.377107 | 5.4:+76131524 | MS.gene020858:CDS |
| TGAATGATTCTGCAGGTAGT+AGG | 0.386036 | 5.4:+76136527 | MS.gene020858:intron |
| GTATGAGGCTGTTGAGTTAC+GGG | 0.389583 | 5.4:+76130437 | MS.gene020858:CDS |
| ATCATAACCGGTTCTACTAA+TGG | 0.389720 | 5.4:-76134307 | None:intergenic |
| CAGTTTATGCTGGTGAAGAT+TGG | 0.398772 | 5.4:+76137975 | MS.gene020858:CDS |
| TTTCGTGCTTCTGTTCCATC+TGG | 0.400465 | 5.4:+76128120 | MS.gene020858:CDS |
| CTATCATAGCATGATCAATC+TGG | 0.408498 | 5.4:-76130566 | None:intergenic |
| TCCATTTGACAAGGAAGATT+GGG | 0.410260 | 5.4:+76135878 | MS.gene020858:CDS |
| GATGATTCATTTCAGGTATG+AGG | 0.417338 | 5.4:+76130422 | MS.gene020858:intron |
| TCCCAATCTTCCTTGTCAAA+TGG | 0.418960 | 5.4:-76135879 | None:intergenic |
| GGTATGAGGCTGTTGAGTTA+CGG | 0.423365 | 5.4:+76130436 | MS.gene020858:intron |
| TGGCCTCCTTTACAAGATCC+AGG | 0.431446 | 5.4:-76134287 | None:intergenic |
| AAAGCTTGCCTGGAGACATT+AGG | 0.436617 | 5.4:-76133259 | None:intergenic |
| AACAGGTAAAAGATATGATC+TGG | 0.438662 | 5.4:+76135650 | MS.gene020858:intron |
| AAAACATGCTGGAAATAATC+TGG | 0.443130 | 5.4:+76131755 | MS.gene020858:CDS |
| GATATTACGATGAAGAGAAC+GGG | 0.448791 | 5.4:-76127896 | None:intergenic |
| GAAATCATGATTCTTCCAAT+TGG | 0.450349 | 5.4:+76132898 | MS.gene020858:CDS |
| TAAAGCTGGGGCTGCTGAAA+AGG | 0.454878 | 5.4:+76131538 | MS.gene020858:CDS |
| GATCTTCTATTGATACAATT+GGG | 0.456790 | 5.4:-76135857 | None:intergenic |
| CTTCGGATTGAGGAAGAGCT+TGG | 0.459854 | 5.4:+76137944 | MS.gene020858:CDS |
| CTCAAGCCTTGGAATTTGCC+AGG | 0.463723 | 5.4:+76135917 | MS.gene020858:CDS |
| GAATGATTCTGCAGGTAGTA+GGG | 0.464232 | 5.4:+76136528 | MS.gene020858:intron |
| TTATCTGTTGGCCTTGCTGT+TGG | 0.466785 | 5.4:+76137220 | MS.gene020858:CDS |
| GGGGACAAAGGTGTATATCT+AGG | 0.471083 | 5.4:+76130462 | MS.gene020858:CDS |
| ATCAAGTATATCTCAAGCCT+TGG | 0.478687 | 5.4:+76135906 | MS.gene020858:CDS |
| CACCAGCTTTAGAGAAGCCC+TGG | 0.479064 | 5.4:+76134269 | MS.gene020858:intron |
| AAGGAGGCCATTAGTAGAAC+CGG | 0.479320 | 5.4:+76134300 | MS.gene020858:CDS |
| GACGGCGTCTTCGAGTTTAC+GGG | 0.479590 | 5.4:-76127843 | None:intergenic |
| AAAATATCTGAGGCGCTGAT+TGG | 0.482231 | 5.4:+76130522 | MS.gene020858:CDS |
| TGGAAATAATCTGGCAATTG+AGG | 0.486178 | 5.4:+76131764 | MS.gene020858:CDS |
| AAGCACATCGCTGACCTGTC+GGG | 0.488085 | 5.4:+76131681 | MS.gene020858:CDS |
| CCATTGCTGCATGTAAAGCT+GGG | 0.490656 | 5.4:+76131525 | MS.gene020858:CDS |
| AAAGCAGGAACAGGAAGCAC+TGG | 0.490991 | 5.4:-76131714 | None:intergenic |
| CCTCGGTAACAGTTCCAACT+TGG | 0.491628 | 5.4:-76137105 | None:intergenic |
| GATACAGTTACTTCGGATTG+AGG | 0.493277 | 5.4:+76137934 | MS.gene020858:intron |
| GGCTGTTGAGTTACGGGATG+GGG | 0.493888 | 5.4:+76130443 | MS.gene020858:CDS |
| ATAACAGTAAAAGCAGGAAC+AGG | 0.498354 | 5.4:-76131723 | None:intergenic |
| GCTGTTGGTCAGATTAAAGC+AGG | 0.505927 | 5.4:+76137235 | MS.gene020858:CDS |
| ATCTGAGGCGCTGATTGGTA+TGG | 0.509293 | 5.4:+76130527 | MS.gene020858:CDS |
| AGCAGGCAAAGGAAGCGCAT+TGG | 0.509873 | 5.4:+76137143 | MS.gene020858:CDS |
| GAAATTTGATACAGTTACTT+CGG | 0.514281 | 5.4:+76137927 | MS.gene020858:intron |
| AACTGTAATGTCGGTGAAGA+TGG | 0.515053 | 5.4:+76133226 | MS.gene020858:CDS |
| TTTAAATCTCCTCAAAAGTC+GGG | 0.515504 | 5.4:+76135675 | MS.gene020858:CDS |
| AAGATCGCTCTTGACGTCGC+TGG | 0.516723 | 5.4:+76134339 | MS.gene020858:CDS |
| ATTTGATGAAGCACTGCGAA+TGG | 0.517360 | 5.4:+76132930 | MS.gene020858:CDS |
| GCTTTGCTCCTAATGTCTCC+AGG | 0.523451 | 5.4:+76133251 | MS.gene020858:CDS |
| GTTCCATCTGGAAACTCCAC+TGG | 0.529470 | 5.4:+76128132 | MS.gene020858:CDS |
| TTTGATGAAGCACTGCGAAT+GGG | 0.531009 | 5.4:+76132931 | MS.gene020858:CDS |
| TACAGATCAACTTCAACAGT+TGG | 0.534257 | 5.4:-76128081 | None:intergenic |
| CTTTAAATCTCCTCAAAAGT+CGG | 0.534503 | 5.4:+76135674 | MS.gene020858:CDS |
| TTTATGCTGGTGAAGATTGG+AGG | 0.538769 | 5.4:+76137978 | MS.gene020858:CDS |
| AGGCAAAGGAAGCGCATTGG+GGG | 0.540352 | 5.4:+76137146 | MS.gene020858:CDS |
| GGAGATCAAGCAGTTTATGC+TGG | 0.544508 | 5.4:+76137965 | MS.gene020858:CDS |
| GGTCATTGAAGTGGTCAAGC+AGG | 0.547758 | 5.4:+76137126 | MS.gene020858:CDS |
| TCACCGACATTACAGTTATG+TGG | 0.549326 | 5.4:-76133220 | None:intergenic |
| TATCATAGCATGATCAATCT+GGG | 0.550616 | 5.4:-76130565 | None:intergenic |
| CAGGCAAAGGAAGCGCATTG+GGG | 0.551427 | 5.4:+76137145 | MS.gene020858:CDS |
| GTGATCCAGGTCAACCAAGT+TGG | 0.555500 | 5.4:+76137091 | MS.gene020858:intron |
| TCTAATCGCTCTCCTCTGCA+AGG | 0.555562 | 5.4:-76137262 | None:intergenic |
| GGCCTCCTTTACAAGATCCA+GGG | 0.557045 | 5.4:-76134286 | None:intergenic |
| AAAGGTGTATATCTAGGAAA+CGG | 0.559992 | 5.4:+76130468 | MS.gene020858:CDS |
| TCAATCTGGGACTGCAGTGT+TGG | 0.560963 | 5.4:-76130552 | None:intergenic |
| AGAAGCCCTGGATCTTGTAA+AGG | 0.562004 | 5.4:+76134281 | MS.gene020858:CDS |
| ATAATACCTGGCAAATTCCA+AGG | 0.562772 | 5.4:-76135923 | None:intergenic |
| AGGCTGTTGAGTTACGGGAT+GGG | 0.576344 | 5.4:+76130442 | MS.gene020858:CDS |
| AGATATTACGATGAAGAGAA+CGG | 0.576440 | 5.4:-76127897 | None:intergenic |
| TGAAGTGGTCAAGCAGGCAA+AGG | 0.584411 | 5.4:+76137132 | MS.gene020858:CDS |
| GATGAAGAGAACGGGGTCGG+AGG | 0.585100 | 5.4:-76127888 | None:intergenic |
| ATAAGCGGTGGAAAACATGC+TGG | 0.585973 | 5.4:+76131744 | MS.gene020858:CDS |
| ATGCCAGTGGAGTTTCCAGA+TGG | 0.588263 | 5.4:-76128135 | None:intergenic |
| TTTATCAAGATATTCTTGAA+CGG | 0.588908 | 5.4:-76127810 | None:intergenic |
| GAGGCTGTTGAGTTACGGGA+TGG | 0.592923 | 5.4:+76130441 | MS.gene020858:CDS |
| AATGATTCTGCAGGTAGTAG+GGG | 0.598973 | 5.4:+76136529 | MS.gene020858:intron |
| TACGATGAAGAGAACGGGGT+CGG | 0.599870 | 5.4:-76127891 | None:intergenic |
| CCCAGCTTTACATGCAGCAA+TGG | 0.606579 | 5.4:-76131525 | None:intergenic |
| TCAGCGATGTGCTTGTAGAG+TGG | 0.606837 | 5.4:-76131672 | None:intergenic |
| GTGGTGACATCTCACAGAAG+TGG | 0.607193 | 5.4:+76137169 | MS.gene020858:CDS |
| CAAGCACATCGCTGACCTGT+CGG | 0.615526 | 5.4:+76131680 | MS.gene020858:CDS |
| AAGCAGGAACAGGAAGCACT+GGG | 0.619719 | 5.4:-76131713 | None:intergenic |
| TTGTACAAGGAACTATGTAC+CGG | 0.620708 | 5.4:+76135732 | MS.gene020858:CDS |
| AGCCCTGGATCTTGTAAAGG+AGG | 0.621208 | 5.4:+76134284 | MS.gene020858:CDS |
| GAGTTACGGGATGGGGACAA+AGG | 0.621649 | 5.4:+76130450 | MS.gene020858:CDS |
| CCGCTTATAACAGTAAAAGC+AGG | 0.623476 | 5.4:-76131729 | None:intergenic |
| AGGCAAATTCTTGATAGTAG+AGG | 0.625871 | 5.4:+76128054 | MS.gene020858:CDS |
| CATTGCTGCATGTAAAGCTG+GGG | 0.626891 | 5.4:+76131526 | MS.gene020858:CDS |
| CCAAGTTGGAACTGTTACCG+AGG | 0.633838 | 5.4:+76137105 | MS.gene020858:CDS |
| AAAGCAGGCGCTCCTTGCAG+AGG | 0.636542 | 5.4:+76137250 | MS.gene020858:CDS |
| ATTAACATCTTACATGCCAG+TGG | 0.640506 | 5.4:-76128148 | None:intergenic |
| CTTGACCACTTCAATGACCT+CGG | 0.640611 | 5.4:-76137122 | None:intergenic |
| TATAAATGAGAAAATATCTG+AGG | 0.641810 | 5.4:+76130512 | MS.gene020858:CDS |
| CTTTAATCTGACCAACAGCA+AGG | 0.642755 | 5.4:-76137231 | None:intergenic |
| TGTTACCGAGGTCATTGAAG+TGG | 0.644212 | 5.4:+76137117 | MS.gene020858:CDS |
| GGCAAAGGAAGCGCATTGGG+GGG | 0.654274 | 5.4:+76137147 | MS.gene020858:CDS |
| GGATATGATAGAATTGTACA+AGG | 0.666629 | 5.4:+76135719 | MS.gene020858:CDS |
| TGATTACGAAAGTAAAAGCG+AGG | 0.674184 | 5.4:+76128034 | MS.gene020858:CDS |
| AATAGAAGATCCATTTGACA+AGG | 0.686135 | 5.4:+76135869 | MS.gene020858:CDS |
| GGGCCACATAACTGTAATGT+CGG | 0.693032 | 5.4:+76133217 | MS.gene020858:CDS |
| TGTAATGTCGGTGAAGATGG+TGG | 0.702441 | 5.4:+76133229 | MS.gene020858:CDS |
| ATATTACGATGAAGAGAACG+GGG | 0.743082 | 5.4:-76127895 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 76127806 | 76138006 | 76127806 | ID=MS.gene020858 |
| chr5.4 | mRNA | 76127806 | 76138006 | 76127806 | ID=MS.gene020858.t1;Parent=MS.gene020858 |
| chr5.4 | exon | 76127806 | 76127910 | 76127806 | ID=MS.gene020858.t1.exon1;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76127806 | 76127910 | 76127806 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76128003 | 76128157 | 76128003 | ID=MS.gene020858.t1.exon2;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76128003 | 76128157 | 76128003 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76130437 | 76130611 | 76130437 | ID=MS.gene020858.t1.exon3;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76130437 | 76130611 | 76130437 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76131491 | 76131562 | 76131491 | ID=MS.gene020858.t1.exon4;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76131491 | 76131562 | 76131491 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76131669 | 76131785 | 76131669 | ID=MS.gene020858.t1.exon5;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76131669 | 76131785 | 76131669 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76132898 | 76132978 | 76132898 | ID=MS.gene020858.t1.exon6;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76132898 | 76132978 | 76132898 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76133196 | 76133272 | 76133196 | ID=MS.gene020858.t1.exon7;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76133196 | 76133272 | 76133196 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76134275 | 76134378 | 76134275 | ID=MS.gene020858.t1.exon8;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76134275 | 76134378 | 76134275 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76135655 | 76135753 | 76135655 | ID=MS.gene020858.t1.exon9;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76135655 | 76135753 | 76135655 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76135853 | 76135938 | 76135853 | ID=MS.gene020858.t1.exon10;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76135853 | 76135938 | 76135853 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76136542 | 76136628 | 76136542 | ID=MS.gene020858.t1.exon11;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76136542 | 76136628 | 76136542 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76137100 | 76137297 | 76137100 | ID=MS.gene020858.t1.exon12;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76137100 | 76137297 | 76137100 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| chr5.4 | exon | 76137941 | 76138006 | 76137941 | ID=MS.gene020858.t1.exon13;Parent=MS.gene020858.t1 |
| chr5.4 | CDS | 76137941 | 76138006 | 76137941 | ID=cds.MS.gene020858.t1;Parent=MS.gene020858.t1 |
| Gene Sequence |
| Protein sequence |
