AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023354

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023354.t1 MTR_8g079940 89.194 546 47 3 1 542 1 538 0.0 994
MS.gene023354.t1 MTR_3g086100 62.238 572 164 10 12 542 1 561 0.0 656
MS.gene023354.t1 MTR_3g086100 59.800 500 150 9 84 542 1 490 0.0 544
MS.gene023354.t1 MTR_1g032570 57.249 269 110 2 25 288 11 279 1.79e-95 303
MS.gene023354.t1 MTR_4g121020 59.690 258 100 1 23 276 29 286 5.22e-93 298
MS.gene023354.t1 MTR_4g121020 59.690 258 100 1 23 276 29 286 5.26e-93 298
MS.gene023354.t1 MTR_2g034960 57.143 252 106 1 23 274 27 276 1.33e-87 282
MS.gene023354.t1 MTR_3g106220 54.373 263 108 2 23 276 6 265 1.81e-86 280
MS.gene023354.t1 MTR_1g013170 55.303 264 96 4 23 274 3 256 8.29e-81 261
MS.gene023354.t1 MTR_4g131600 51.984 252 112 4 23 271 18 263 3.98e-78 257
MS.gene023354.t1 MTR_4g131580 50.958 261 101 4 23 270 15 261 8.27e-76 251
MS.gene023354.t1 MTR_2g450070 47.152 316 127 6 22 298 15 329 1.76e-75 252
MS.gene023354.t1 MTR_2g450070 47.152 316 127 6 22 298 15 329 2.35e-75 252
MS.gene023354.t1 MTR_4g131570 52.016 248 117 1 25 270 17 264 1.32e-74 248
MS.gene023354.t1 MTR_1g013180 54.365 252 101 3 23 266 154 399 5.53e-73 245
MS.gene023354.t1 MTR_7g117705 48.000 275 121 4 23 285 3 267 7.64e-72 237
MS.gene023354.t1 MTR_1g032570 59.788 189 71 2 84 267 1 189 2.89e-67 227
MS.gene023354.t1 MTR_3g102600 45.487 277 83 4 23 298 6 215 2.64e-65 212
MS.gene023354.t1 MTR_3g102600 47.059 238 66 2 23 260 6 183 5.30e-60 197
MS.gene023354.t1 MTR_4g021760 41.085 258 121 6 24 281 10 236 1.87e-57 194
MS.gene023354.t1 MTR_4g021790 42.798 243 113 4 24 266 9 225 3.09e-56 190
MS.gene023354.t1 MTR_3g102590 45.736 258 87 4 23 277 6 213 4.15e-54 183
MS.gene023354.t1 MTR_4g021845 40.426 235 114 4 24 258 9 217 2.99e-53 180
MS.gene023354.t1 MTR_3g102600 43.534 232 63 4 68 298 2 166 4.43e-52 176
MS.gene023354.t1 MTR_4g021855 39.506 243 121 3 24 266 9 225 6.09e-52 179
MS.gene023354.t1 MTR_3g102590 46.414 237 76 3 23 258 6 192 5.39e-50 171
MS.gene023354.t1 MTR_3g102600 45.078 193 46 2 68 260 2 134 2.97e-47 161
MS.gene023354.t1 MTR_4g121020 51.977 177 81 1 104 276 6 182 1.39e-46 172
MS.gene023354.t1 MTR_6g045327 36.364 242 124 5 25 264 14 227 9.10e-43 157
MS.gene023354.t1 MTR_0450s0040 34.643 280 156 5 27 303 6 261 3.05e-42 154
MS.gene023354.t1 MTR_8g105600 36.220 254 154 3 23 275 7 253 3.60e-42 159
MS.gene023354.t1 MTR_1g013160 48.734 158 59 4 87 232 1 148 1.94e-34 127
MS.gene023354.t1 MTR_7g026400 34.310 239 143 3 55 293 17 241 1.06e-33 135
MS.gene023354.t1 MTR_5g055260 31.276 243 114 5 24 266 9 198 1.16e-31 123
MS.gene023354.t1 MTR_8g093040 31.579 247 128 4 25 271 14 219 1.41e-28 115
MS.gene023354.t1 MTR_4g023980 33.129 163 84 2 72 234 36 173 8.47e-27 107
MS.gene023354.t1 MTR_8g032710 29.412 255 158 7 27 261 19 271 4.88e-24 105
MS.gene023354.t1 MTR_6g016850 34.266 143 86 4 25 160 9 150 3.95e-23 96.7
MS.gene023354.t1 MTR_3g092780 35.673 171 96 5 29 194 56 217 7.83e-23 102
MS.gene023354.t1 MTR_3g092780 35.673 171 96 5 29 194 56 217 8.27e-23 103
MS.gene023354.t1 MTR_3g092780 35.673 171 95 5 29 194 56 216 2.40e-22 101
MS.gene023354.t1 MTR_3g092780 35.673 171 95 5 29 194 56 216 2.63e-22 101
MS.gene023354.t1 MTR_7g118260 37.500 128 71 2 26 147 11 135 1.97e-20 95.5
MS.gene023354.t1 MTR_7g118260 37.500 128 71 2 26 147 11 135 2.13e-20 95.1
MS.gene023354.t1 MTR_4g061360 36.585 123 73 2 29 147 89 210 6.63e-19 90.9
MS.gene023354.t1 MTR_3g037390 34.146 123 78 1 29 148 24 146 2.44e-18 88.6
MS.gene023354.t1 MTR_8g024260 36.585 123 73 2 31 149 38 159 7.57e-18 87.4
MS.gene023354.t1 MTR_4g064730 58.333 72 29 1 207 277 129 200 8.65e-18 84.7
MS.gene023354.t1 MTR_1g067110 36.066 122 75 1 29 147 85 206 1.45e-17 86.7
MS.gene023354.t1 MTR_1g067110 36.066 122 75 1 29 147 85 206 1.70e-17 86.7
MS.gene023354.t1 MTR_4g108880 34.959 123 77 1 29 148 33 155 1.91e-17 85.9
MS.gene023354.t1 MTR_7g089010 59.677 62 25 0 207 268 89 150 1.12e-16 81.3
MS.gene023354.t1 MTR_4g098870 54.795 73 32 1 208 279 309 381 1.06e-14 77.4
MS.gene023354.t1 MTR_4g098870 35.484 124 76 2 25 147 14 134 2.17e-13 73.2
MS.gene023354.t1 MTR_4g098870 54.795 73 32 1 208 279 329 401 1.10e-14 77.4
MS.gene023354.t1 MTR_4g098870 35.484 124 76 2 25 147 34 154 3.00e-13 72.8
MS.gene023354.t1 MTR_4g098870 54.795 73 32 1 208 279 348 420 1.16e-14 77.4
MS.gene023354.t1 MTR_4g098870 35.484 124 76 2 25 147 53 173 3.04e-13 72.8
MS.gene023354.t1 MTR_4g098870 54.795 73 32 1 208 279 309 381 1.18e-14 77.0
MS.gene023354.t1 MTR_4g098870 35.484 124 76 2 25 147 14 134 2.93e-13 72.8
MS.gene023354.t1 MTR_4g098870 54.795 73 32 1 208 279 329 401 1.23e-14 77.0
MS.gene023354.t1 MTR_4g098870 35.484 124 76 2 25 147 34 154 2.68e-13 72.8
MS.gene023354.t1 MTR_5g014040 62.500 56 21 0 208 263 310 365 1.93e-14 76.3
MS.gene023354.t1 MTR_5g014040 36.800 125 73 3 25 147 14 134 2.42e-13 72.8
MS.gene023354.t1 MTR_5g014040 36.800 125 73 3 25 147 14 134 2.36e-13 73.2
MS.gene023354.t1 MTR_5g014040 57.377 61 21 1 208 263 310 370 1.74e-12 70.1
MS.gene023354.t1 MTR_2g090675 46.377 69 37 0 208 276 160 228 3.23e-13 72.0
MS.gene023354.t1 MTR_4g078810 46.377 69 37 0 208 276 157 225 4.18e-13 71.6
MS.gene023354.t1 MTR_4g106590 30.070 143 84 4 26 152 20 162 7.27e-13 68.6
MS.gene023354.t1 MTR_8g038620 29.861 144 81 3 31 154 11 154 1.29e-12 66.6
MS.gene023354.t1 MTR_5g014040 57.377 61 21 1 208 263 270 330 1.52e-12 70.5
MS.gene023354.t1 MTR_7g088070 49.315 73 34 2 209 278 50 122 5.72e-12 67.0
MS.gene023354.t1 MTR_6g088950 32.941 85 57 0 64 148 43 127 8.42e-12 65.1
MS.gene023354.t1 MTR_7g490310 25.294 170 104 3 24 170 4 173 9.24e-12 65.5
MS.gene023354.t1 MTR_1g093080 46.479 71 37 1 208 277 210 280 1.85e-11 66.2
MS.gene023354.t1 MTR_6g032990 54.545 55 24 1 209 262 42 96 2.06e-11 66.2
MS.gene023354.t1 MTR_2g086450 46.667 75 33 2 209 282 45 113 2.58e-11 65.9
MS.gene023354.t1 MTR_5g041350 52.727 55 25 1 209 262 15 69 4.04e-11 65.1
MS.gene023354.t1 MTR_6g453220 75.000 44 11 0 224 267 378 421 5.14e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023354.t1 AT1G67710 49.299 499 199 15 26 507 8 469 8.63e-126 379
MS.gene023354.t1 AT1G67710 45.125 441 188 15 84 507 1 404 2.08e-90 285
MS.gene023354.t1 AT3G16857 53.289 304 115 7 22 302 30 329 1.40e-86 281
MS.gene023354.t1 AT3G16857 53.289 304 115 7 22 302 30 329 2.86e-86 281
MS.gene023354.t1 AT2G01760 52.416 269 107 4 20 273 2 264 1.98e-82 262
MS.gene023354.t1 AT2G25180 54.826 259 103 2 22 274 10 260 6.30e-82 267
MS.gene023354.t1 AT2G01760 47.475 297 107 5 20 273 2 292 4.23e-76 246
MS.gene023354.t1 AT4G31920 50.943 265 104 4 22 280 10 254 3.22e-75 248
MS.gene023354.t1 AT1G67710 42.280 421 189 15 104 507 12 395 1.01e-73 241
MS.gene023354.t1 AT3G16857 52.290 262 98 7 64 302 2 259 1.21e-67 230
MS.gene023354.t1 AT5G58080 50.000 258 117 4 23 276 12 261 2.01e-65 224
MS.gene023354.t1 AT2G01760 50.732 205 80 4 84 273 1 199 1.15e-56 192
MS.gene023354.t1 AT5G49240 39.407 236 140 2 25 258 38 272 6.88e-47 166
MS.gene023354.t1 AT1G49190 33.083 266 145 5 22 268 27 278 5.20e-38 144
MS.gene023354.t1 AT5G07210 32.601 273 148 7 29 270 16 283 3.30e-34 137
MS.gene023354.t1 AT3G62670 34.728 239 137 5 30 261 42 268 9.47e-33 130
MS.gene023354.t1 AT5G07210 31.673 281 148 9 29 270 16 291 1.89e-32 132
MS.gene023354.t1 AT2G27070 30.584 291 159 6 29 283 16 299 5.63e-31 127
MS.gene023354.t1 AT1G49190 41.406 128 75 0 22 149 27 154 1.49e-26 114
MS.gene023354.t1 AT5G24470 38.400 125 72 2 28 148 49 172 1.27e-21 99.0
MS.gene023354.t1 AT2G46790 38.211 123 71 2 29 147 37 158 1.94e-21 97.4
MS.gene023354.t1 AT2G46790 38.211 123 71 2 29 147 37 158 2.18e-21 97.8
MS.gene023354.t1 AT5G60100 39.855 138 77 3 29 162 64 199 4.42e-20 93.2
MS.gene023354.t1 AT5G60100 39.855 138 77 3 29 162 64 199 4.42e-20 93.2
MS.gene023354.t1 AT5G60100 39.855 138 77 3 29 162 64 199 4.42e-20 93.2
MS.gene023354.t1 AT1G68210 25.091 275 163 4 29 267 13 280 6.32e-20 94.4
MS.gene023354.t1 AT5G60100 40.310 129 71 3 29 153 64 190 6.37e-20 93.6
MS.gene023354.t1 AT5G60100 39.855 138 77 3 29 162 64 199 7.60e-20 93.2
MS.gene023354.t1 AT5G60100 39.855 138 77 3 29 162 64 199 7.60e-20 93.2
MS.gene023354.t1 AT5G60100 39.855 138 77 3 29 162 64 199 7.60e-20 93.2
MS.gene023354.t1 AT5G61380 36.585 123 75 1 29 148 19 141 1.37e-18 89.7
MS.gene023354.t1 AT3G46640 50.633 79 38 1 199 277 135 212 2.09e-17 84.0
MS.gene023354.t1 AT3G46640 50.633 79 38 1 199 277 135 212 2.09e-17 84.0
MS.gene023354.t1 AT3G46640 53.521 71 33 0 207 277 142 212 3.56e-17 83.2
MS.gene023354.t1 AT2G40970 59.677 62 25 0 207 268 103 164 4.03e-17 81.6
MS.gene023354.t1 AT5G59570 60.656 61 24 0 208 268 140 200 5.84e-17 82.0
MS.gene023354.t1 AT5G59570 60.656 61 24 0 208 268 140 200 5.84e-17 82.0
MS.gene023354.t1 AT5G02810 38.211 123 71 2 29 147 78 199 6.40e-17 84.7
MS.gene023354.t1 AT5G05090 46.667 105 53 2 207 311 79 180 6.95e-17 81.3
MS.gene023354.t1 AT3G10760 59.677 62 25 0 207 268 103 164 7.86e-17 82.4
MS.gene023354.t1 AT5G44190 50.000 76 38 0 208 283 146 221 3.93e-15 77.4
MS.gene023354.t1 AT5G44190 50.000 76 38 0 208 283 146 221 4.01e-15 77.8
MS.gene023354.t1 AT2G20570 48.000 75 39 0 208 282 152 226 2.10e-14 75.9
MS.gene023354.t1 AT2G20570 45.783 83 45 0 200 282 160 242 5.58e-14 74.7
MS.gene023354.t1 AT3G57040 28.313 166 96 4 24 167 4 168 9.58e-14 71.2
MS.gene023354.t1 AT3G57040 28.313 166 96 4 24 167 4 168 9.58e-14 71.2
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 4.83e-13 72.0
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 9.60e-13 70.9
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 4.83e-13 72.0
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 9.60e-13 70.9
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 4.83e-13 72.0
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 9.60e-13 70.9
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 4.83e-13 72.0
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 9.60e-13 70.9
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 4.83e-13 72.0
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 9.60e-13 70.9
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 4.83e-13 72.0
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 9.60e-13 70.9
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 6.96e-13 71.6
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 8.08e-13 71.2
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 6.96e-13 71.6
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 8.08e-13 71.2
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 6.96e-13 71.6
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 8.08e-13 71.2
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 6.96e-13 71.6
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 8.08e-13 71.2
MS.gene023354.t1 AT4G18020 34.074 135 80 3 25 152 14 146 6.96e-13 71.6
MS.gene023354.t1 AT4G18020 50.769 65 32 0 209 273 296 360 8.08e-13 71.2
MS.gene023354.t1 AT5G18240 56.364 55 23 1 209 262 45 99 8.00e-12 67.8
MS.gene023354.t1 AT5G18240 56.364 55 23 1 209 262 45 99 8.00e-12 67.8
MS.gene023354.t1 AT5G18240 56.364 55 23 1 209 262 45 99 8.36e-12 67.8
MS.gene023354.t1 AT5G18240 56.364 55 23 1 209 262 45 99 8.82e-12 67.4
MS.gene023354.t1 AT5G18240 56.364 55 23 1 209 262 45 99 8.82e-12 67.4
MS.gene023354.t1 AT3G04030 54.545 55 24 1 209 262 45 99 1.14e-11 67.0
MS.gene023354.t1 AT3G04030 54.545 55 24 1 209 262 45 99 1.37e-11 67.0

Find 85 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAACTCTTTCTTATCTT+TGG 0.198401 4.4:+16845108 None:intergenic
TCTTGGTTGCATTTATGATA+TGG 0.215616 4.4:-16844716 MS.gene023354:CDS
TGTTGCTGTGTAACATTTAA+TGG 0.220612 4.4:+16844931 None:intergenic
TTGAAAGTGATTGGTCAATT+TGG 0.229447 4.4:+16844905 None:intergenic
ATCATTTGAGATGAGGCTTT+TGG 0.254966 4.4:+16844795 None:intergenic
GAACACACTTGTACAATTAA+AGG 0.258335 4.4:-16844971 MS.gene023354:CDS
ATTATTTGCTTAAGCCTATA+AGG 0.266042 4.4:-16846175 MS.gene023354:CDS
CGCAATTCTTTCATCCTTAT+AGG 0.272142 4.4:+16846161 None:intergenic
TACCCAAAAGTAAGTTTCTT+TGG 0.274466 4.4:+16844686 None:intergenic
CTACCAAAGAAACTTACTTT+TGG 0.276715 4.4:-16844689 MS.gene023354:CDS
TGGAATTAATTGAAAGTGAT+TGG 0.324987 4.4:+16844896 None:intergenic
TTTGAAGTTAAAATGATATC+AGG 0.326062 4.4:+16844766 None:intergenic
AAGTTCGATCTACGAGTTCT+TGG 0.327718 4.4:+16845958 None:intergenic
GAAGGTGCAATCGATAGTTT+TGG 0.347432 4.4:-16845213 MS.gene023354:CDS
ATCTGCTGAAGCATACAAAT+TGG 0.351333 4.4:+16844528 None:intergenic
TGAGTCAGCTTCCACACCTT+TGG 0.366813 4.4:-16844662 MS.gene023354:CDS
AAATGTTGCTAGTCACTTGC+AGG 0.372643 4.4:-16845617 MS.gene023354:intron
TAAAGAAAGCTAGAGTAGTT+TGG 0.378977 4.4:-16845932 MS.gene023354:CDS
AATGTAATCATTCTTGTAAT+CGG 0.392849 4.4:+16844867 None:intergenic
GGAATTCACTTAATGAGAAA+TGG 0.405209 4.4:-16846074 MS.gene023354:CDS
TTACTTCTGATTCCAGAGAT+TGG 0.407149 4.4:+16845073 None:intergenic
AAAACACCAAGATTTAATCT+TGG 0.410977 4.4:-16844733 MS.gene023354:CDS
CACATGTTGCTTAGCAATAG+AGG 0.420963 4.4:-16846597 MS.gene023354:CDS
CCATACCAACACAGTACTCT+TGG 0.432925 4.4:-16845029 MS.gene023354:CDS
ATTCTTGTAATCGGAGGGAT+TGG 0.437838 4.4:+16844876 None:intergenic
CATATGCATCTTTACTTTCG+CGG 0.445631 4.4:+16846561 None:intergenic
AGCTTGAAACCATCCATGTC+AGG 0.458164 4.4:+16846512 None:intergenic
GGTAGGTCTTAACCAATCTC+TGG 0.459702 4.4:-16845085 MS.gene023354:CDS
CATATTCACCTTGTAACAAA+AGG 0.468727 4.4:+16844623 None:intergenic
AAATGAACTTAATGAAGAAA+AGG 0.469526 4.4:-16846007 MS.gene023354:CDS
ATGCAACCAAGATTAAATCT+TGG 0.480002 4.4:+16844727 None:intergenic
TGAAAGAATTGCGAAACATA+TGG 0.480046 4.4:-16846151 MS.gene023354:CDS
GATTTACCTTCAAAAGATCC+AGG 0.483223 4.4:-16845276 MS.gene023354:CDS
TTCAAGCTTCTTGAACATGT+TGG 0.489231 4.4:-16846497 MS.gene023354:CDS
TTGAGAAAGCTTAAACATCC+TGG 0.491396 4.4:+16845258 None:intergenic
CAAACATCATGGTACACAAC+AGG 0.500098 4.4:-16847294 None:intergenic
TATGCAGTGATGTCTGTTGA+TGG 0.505268 4.4:-16846242 MS.gene023354:intron
AGCTTCCACACCTTTGGACG+AGG 0.506847 4.4:-16844656 MS.gene023354:CDS
GTTAAAGCTGTGAATCAACT+TGG 0.507253 4.4:-16845885 MS.gene023354:CDS
GGATCAGATCACACTGATGA+TGG 0.509373 4.4:-16846053 MS.gene023354:CDS
ACGAGAACTCGTAGACCAGC+TGG 0.523070 4.4:+16847211 None:intergenic
CATTAGTCCTGTGAATTCAT+CGG 0.523463 4.4:-16844848 MS.gene023354:CDS
TGAACATGTTGGACTTGAGA+TGG 0.525184 4.4:-16846486 MS.gene023354:CDS
AAAATTCAAATGGATACTAC+AGG 0.527020 4.4:-16845171 MS.gene023354:CDS
CAAATGGATACTACAGGTCA+TGG 0.528328 4.4:-16845165 MS.gene023354:CDS
GAGATTGATCATTTGAGATG+AGG 0.529752 4.4:+16844788 None:intergenic
GTGTAACATTTAATGGCAAA+TGG 0.530650 4.4:+16844938 None:intergenic
GGAACTTCAGCAATAAACCT+TGG 0.534763 4.4:+16844553 None:intergenic
TAAAAGAACTTACTAATGAC+AGG 0.538868 4.4:+16846458 None:intergenic
GTGTTCTGTTCTTCTTCGAG+TGG 0.552522 4.4:+16844988 None:intergenic
CGATGTAAACATGCCTGACA+TGG 0.555999 4.4:-16846525 MS.gene023354:CDS
GCAACCTACTCAAGTATAGC+CGG 0.557803 4.4:+16845346 None:intergenic
TAATCATTCTTGTAATCGGA+GGG 0.562863 4.4:+16844871 None:intergenic
TGGTGGAGTCAAATGCTGTA+TGG 0.564365 4.4:+16845049 None:intergenic
AAAGCATCAAGGTGTGGAGA+AGG 0.568756 4.4:+16847235 None:intergenic
CCAAGAGTACTGTGTTGGTA+TGG 0.569363 4.4:+16845029 None:intergenic
AATGATCAGAAATTGTCCTC+AGG 0.572148 4.4:-16845315 MS.gene023354:CDS
GCTGAAGCATACAAATTGGT+AGG 0.582128 4.4:+16844532 None:intergenic
TCATTCCAAGAGTACTGTGT+TGG 0.583367 4.4:+16845024 None:intergenic
TGTCAGAGTATTATGATCCA+AGG 0.585355 4.4:-16844570 MS.gene023354:CDS
CTGTTGATGGAGAAACAAGC+AGG 0.593651 4.4:-16846229 MS.gene023354:CDS
GTGATGAAAGGTGTTCAACA+TGG 0.594525 4.4:-16846206 MS.gene023354:CDS
GTAATCATTCTTGTAATCGG+AGG 0.596210 4.4:+16844870 None:intergenic
ATAAGAAAGAGTTCAGAGGT+AGG 0.597933 4.4:-16845102 MS.gene023354:CDS
AAGTTTAAGTCCTCGTCCAA+AGG 0.599539 4.4:+16844646 None:intergenic
AGAGTACTGTGTTGGTATGG+TGG 0.600173 4.4:+16845032 None:intergenic
GACCAGCTGGAAAAGCATCA+AGG 0.602271 4.4:+16847224 None:intergenic
TTGTTGATGATGATCTAACT+TGG 0.602339 4.4:-16847189 MS.gene023354:CDS
AGCTAGAGTAGTTTGGTCTG+TGG 0.615227 4.4:-16845925 MS.gene023354:CDS
GAAGACACCGATGAATTCAC+AGG 0.619719 4.4:+16844841 None:intergenic
AATACCGGCTATACTTGAGT+AGG 0.620491 4.4:-16845350 MS.gene023354:CDS
CTCTATTGCTAAGCAACATG+TGG 0.620636 4.4:+16846598 None:intergenic
TAAGTCCTCGTCCAAAGGTG+TGG 0.621176 4.4:+16844651 None:intergenic
AAAGATAAGAAAGAGTTCAG+AGG 0.625719 4.4:-16845106 MS.gene023354:CDS
GAAACAAGCAGGGTGATGAA+AGG 0.627020 4.4:-16846218 MS.gene023354:CDS
GCTGGAAAAGCATCAAGGTG+TGG 0.628160 4.4:+16847229 None:intergenic
GTCTCTTGTCTATGACAACA+TGG 0.628850 4.4:-16847272 MS.gene023354:CDS
AAGAGAATTTGAAAGTCTCG+AGG 0.633493 4.4:-16846096 MS.gene023354:CDS
TGAATGCTTTATTCTACCTG+AGG 0.641744 4.4:+16845299 None:intergenic
GATGGTAACATGTTTGCTGT+AGG 0.648059 4.4:-16846035 MS.gene023354:CDS
TAGATTTGATGAATGTTCCG+TGG 0.652142 4.4:-16845651 MS.gene023354:CDS
TTCAGCAAGCAACAAAACGA+AGG 0.652173 4.4:-16845231 MS.gene023354:CDS
AGAGAATTTGAAAGTCTCGA+GGG 0.654792 4.4:-16846095 MS.gene023354:CDS
GTAAACATGCCTGACATGGA+TGG 0.678855 4.4:-16846521 MS.gene023354:CDS
TGTTGATGGAGAAACAAGCA+GGG 0.723682 4.4:-16846228 MS.gene023354:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTTTAGTATAAATT+AGG + chr4.4:16845094-16845113 None:intergenic 10.0%
!!! TGATAAAAATAATTTCTTTT+TGG - chr4.4:16846107-16846126 MS.gene023354:CDS 10.0%
!!! TTTTCTTTGAAAAAAAAAAA+AGG + chr4.4:16844858-16844877 None:intergenic 10.0%
!! TTGAAAAAAAAAAAAGGAAT+TGG + chr4.4:16844852-16844871 None:intergenic 15.0%
!!! AAGAGCAAAATTTTATTATT+AGG + chr4.4:16846431-16846450 None:intergenic 15.0%
!!! ATTTTTTTAGCATTTTTTCA+AGG + chr4.4:16844650-16844669 None:intergenic 15.0%
!!! TCTTTTAACTTAATTAACAA+AGG - chr4.4:16844959-16844978 MS.gene023354:CDS 15.0%
!!! TTTCTTAAATTTGCAAATTT+TGG - chr4.4:16845127-16845146 MS.gene023354:CDS 15.0%
!! AAATGAACTTAATGAAGAAA+AGG - chr4.4:16845798-16845817 MS.gene023354:intron 20.0%
!! AATGTAATCATTCTTGTAAT+CGG + chr4.4:16846941-16846960 None:intergenic 20.0%
!!! AAGTGTTGTTTGTAAAATAA+TGG + chr4.4:16845535-16845554 None:intergenic 20.0%
!!! ATCAAATCTAGAATTTTCTT+GGG + chr4.4:16846144-16846163 None:intergenic 20.0%
!!! TAAACTTTTAACTTTAGTAG+AGG + chr4.4:16846235-16846254 None:intergenic 20.0%
!!! TTTGAAGTTAAAATGATATC+AGG + chr4.4:16847042-16847061 None:intergenic 20.0%
! AAAACACCAAGATTTAATCT+TGG - chr4.4:16847072-16847091 MS.gene023354:intron 25.0%
! AAAATTCAAATGGATACTAC+AGG - chr4.4:16846634-16846653 MS.gene023354:intron 25.0%
! ATTATTTGCTTAAGCCTATA+AGG - chr4.4:16845630-16845649 MS.gene023354:CDS 25.0%
! CTTAAGAAAAAGAATACATG+AGG - chr4.4:16845685-16845704 MS.gene023354:intron 25.0%
! TAAAAGAACTTACTAATGAC+AGG + chr4.4:16845350-16845369 None:intergenic 25.0%
! TGGAATTAATTGAAAGTGAT+TGG + chr4.4:16846912-16846931 None:intergenic 25.0%
! TGGGACATTAAAAATTCAAA+TGG - chr4.4:16846624-16846643 MS.gene023354:intron 25.0%
! TTGAATTGTACTGAACTTAT+TGG + chr4.4:16844776-16844795 None:intergenic 25.0%
!! TTAAACTTCCTTTTGTTACA+AGG - chr4.4:16847174-16847193 MS.gene023354:CDS 25.0%
!!! AATATTTCTGAGATGTGTAT+TGG + chr4.4:16846341-16846360 None:intergenic 25.0%
!!! ATAGTTTTGGATATTCAGAT+GGG - chr4.4:16846605-16846624 MS.gene023354:intron 25.0%
!!! CATCAAATCTAGAATTTTCT+TGG + chr4.4:16846145-16846164 None:intergenic 25.0%
!!! TACCAAAGAAACTTACTTTT+GGG - chr4.4:16847117-16847136 MS.gene023354:intron 25.0%
!!! TGTCTTCTAGCTTTTTTTTT+CGG + chr4.4:16845464-16845483 None:intergenic 25.0%
!!! TTTTGAGTTTGAAAACTTAC+AGG - chr4.4:16846403-16846422 MS.gene023354:intron 25.0%
AAAGATAAGAAAGAGTTCAG+AGG - chr4.4:16846699-16846718 MS.gene023354:intron 30.0%
ATGCAACCAAGATTAAATCT+TGG + chr4.4:16847081-16847100 None:intergenic 30.0%
ATTAGAAGCCAACAAAAATG+TGG + chr4.4:16844827-16844846 None:intergenic 30.0%
CATATTCACCTTGTAACAAA+AGG + chr4.4:16847185-16847204 None:intergenic 30.0%
CTCTTACACAAAAGATATGA+TGG + chr4.4:16846080-16846099 None:intergenic 30.0%
GAACACACTTGTACAATTAA+AGG - chr4.4:16846834-16846853 MS.gene023354:intron 30.0%
GGAATTCACTTAATGAGAAA+TGG - chr4.4:16845731-16845750 MS.gene023354:intron 30.0%
GTACCTACTAGTAATATTCT+TGG - chr4.4:16845437-16845456 MS.gene023354:intron 30.0%
GTAGATTTATTTAGTGTGCT+TGG + chr4.4:16846010-16846029 None:intergenic 30.0%
GTGTAACATTTAATGGCAAA+TGG + chr4.4:16846870-16846889 None:intergenic 30.0%
TAAAGAAAGCTAGAGTAGTT+TGG - chr4.4:16845873-16845892 MS.gene023354:intron 30.0%
TAATCATTCTTGTAATCGGA+GGG + chr4.4:16846937-16846956 None:intergenic 30.0%
TACCCAAAAGTAAGTTTCTT+TGG + chr4.4:16847122-16847141 None:intergenic 30.0%
TCTGAACTCTTTCTTATCTT+TGG + chr4.4:16846700-16846719 None:intergenic 30.0%
TCTTGGTTGCATTTATGATA+TGG - chr4.4:16847089-16847108 MS.gene023354:intron 30.0%
TGAAAGAATTGCGAAACATA+TGG - chr4.4:16845654-16845673 MS.gene023354:CDS 30.0%
TGAATGAAAGAAAGAAACCT+CGG + chr4.4:16844680-16844699 None:intergenic 30.0%
TGTTGCTGTGTAACATTTAA+TGG + chr4.4:16846877-16846896 None:intergenic 30.0%
! ATTTTGCTCTTCAGAAATAC+CGG - chr4.4:16846440-16846459 MS.gene023354:intron 30.0%
! CTAAAAAAATGCTTTTACCG+AGG - chr4.4:16844660-16844679 MS.gene023354:CDS 30.0%
! CTACCAAAGAAACTTACTTT+TGG - chr4.4:16847116-16847135 MS.gene023354:intron 30.0%
! TTGAAAGTGATTGGTCAATT+TGG + chr4.4:16846903-16846922 None:intergenic 30.0%
!! AATTTCTTTTTGGCAGAAGT+TGG - chr4.4:16846117-16846136 MS.gene023354:CDS 30.0%
!! TATATACCACTTTTAGCACA+TGG + chr4.4:16844723-16844742 None:intergenic 30.0%
!! TCTGATATGTTGTTTCTGTT+AGG - chr4.4:16846271-16846290 MS.gene023354:intron 30.0%
!! TTGTTGATGATGATCTAACT+TGG - chr4.4:16844616-16844635 MS.gene023354:CDS 30.0%
!!! GATAGTTTTGGATATTCAGA+TGG - chr4.4:16846604-16846623 MS.gene023354:CDS 30.0%
AAGAGAATTTGAAAGTCTCG+AGG - chr4.4:16845709-16845728 MS.gene023354:intron 35.0%
AAGCCAACAAAAATGTGGAT+TGG + chr4.4:16844822-16844841 None:intergenic 35.0%
AATGATCAGAAATTGTCCTC+AGG - chr4.4:16846490-16846509 MS.gene023354:CDS 35.0%
AGAGAATTTGAAAGTCTCGA+GGG - chr4.4:16845710-16845729 MS.gene023354:intron 35.0%
ATAAGAAAGAGTTCAGAGGT+AGG - chr4.4:16846703-16846722 MS.gene023354:intron 35.0%
ATCTGCTGAAGCATACAAAT+TGG + chr4.4:16847280-16847299 None:intergenic 35.0%
CATATGCATCTTTACTTTCG+CGG + chr4.4:16845247-16845266 None:intergenic 35.0%
CATTAGTCCTGTGAATTCAT+CGG - chr4.4:16846957-16846976 MS.gene023354:intron 35.0%
CGCAATTCTTTCATCCTTAT+AGG + chr4.4:16845647-16845666 None:intergenic 35.0%
CTAGGATGAGATGATAATAC+TGG - chr4.4:16844934-16844953 MS.gene023354:CDS 35.0%
GATTTACCTTCAAAAGATCC+AGG - chr4.4:16846529-16846548 MS.gene023354:CDS 35.0%
GGACCAAGAATATTACTAGT+AGG + chr4.4:16845443-16845462 None:intergenic 35.0%
GTAATCATTCTTGTAATCGG+AGG + chr4.4:16846938-16846957 None:intergenic 35.0%
GTTAAAGCTGTGAATCAACT+TGG - chr4.4:16845920-16845939 MS.gene023354:CDS 35.0%
TAGATTTGATGAATGTTCCG+TGG - chr4.4:16846154-16846173 MS.gene023354:CDS 35.0%
TTACTTCTGATTCCAGAGAT+TGG + chr4.4:16846735-16846754 None:intergenic 35.0%
TTCAAGCTTCTTGAACATGT+TGG - chr4.4:16845308-16845327 MS.gene023354:CDS 35.0%
TTGAGAAAGCTTAAACATCC+TGG + chr4.4:16846550-16846569 None:intergenic 35.0%
! AAACATCCTGGATCTTTTGA+AGG + chr4.4:16846538-16846557 None:intergenic 35.0%
! GAGATTGATCATTTGAGATG+AGG + chr4.4:16847020-16847039 None:intergenic 35.0%
! TACGACTCATGTGTCTTATT+TGG - chr4.4:16845031-16845050 MS.gene023354:CDS 35.0%
! TCACCAATCCACATTTTTGT+TGG - chr4.4:16844816-16844835 MS.gene023354:CDS 35.0%
! TGAATGCTTTATTCTACCTG+AGG + chr4.4:16846509-16846528 None:intergenic 35.0%
! TGTCAGAGTATTATGATCCA+AGG - chr4.4:16847235-16847254 MS.gene023354:CDS 35.0%
!! ATCATTTGAGATGAGGCTTT+TGG + chr4.4:16847013-16847032 None:intergenic 35.0%
!! TATTACTAGTAGGTACTGAC+TGG + chr4.4:16845433-16845452 None:intergenic 35.0%
AAATGTTGCTAGTCACTTGC+AGG - chr4.4:16846188-16846207 MS.gene023354:CDS 40.0%
AAGTTCGATCTACGAGTTCT+TGG + chr4.4:16845850-16845869 None:intergenic 40.0%
AAGTTTAAGTCCTCGTCCAA+AGG + chr4.4:16847162-16847181 None:intergenic 40.0%
ACTAACCAATAACGCTAGCT+AGG - chr4.4:16844916-16844935 MS.gene023354:CDS 40.0%
AGTCTTAATAGCTGCGTGAT+GGG - chr4.4:16845065-16845084 MS.gene023354:CDS 40.0%
ATTCTTGTAATCGGAGGGAT+TGG + chr4.4:16846932-16846951 None:intergenic 40.0%
CAAATGGATACTACAGGTCA+TGG - chr4.4:16846640-16846659 MS.gene023354:intron 40.0%
CTAGAACTAGAAGGCTAGTA+GGG - chr4.4:16846295-16846314 MS.gene023354:intron 40.0%
CTCTATTGCTAAGCAACATG+TGG + chr4.4:16845210-16845229 None:intergenic 40.0%
CTTCTTCCATGTGCTAAAAG+TGG - chr4.4:16844714-16844733 MS.gene023354:CDS 40.0%
GCTGAAGCATACAAATTGGT+AGG + chr4.4:16847276-16847295 None:intergenic 40.0%
GGAACTTCAGCAATAAACCT+TGG + chr4.4:16847255-16847274 None:intergenic 40.0%
GTCTCTTGTCTATGACAACA+TGG - chr4.4:16844533-16844552 MS.gene023354:CDS 40.0%
TAGAACTAGAAGGCTAGTAG+GGG - chr4.4:16846296-16846315 MS.gene023354:intron 40.0%
TATGCAGTGATGTCTGTTGA+TGG - chr4.4:16845563-16845582 MS.gene023354:intron 40.0%
TGAACATGTTGGACTTGAGA+TGG - chr4.4:16845319-16845338 MS.gene023354:CDS 40.0%
TTCAGCAAGCAACAAAACGA+AGG - chr4.4:16846574-16846593 MS.gene023354:CDS 40.0%
! AATACCGGCTATACTTGAGT+AGG - chr4.4:16846455-16846474 MS.gene023354:intron 40.0%
! ACATTTTCTCTAGTCAGCCA+CGG + chr4.4:16846174-16846193 None:intergenic 40.0%
! CACATGTTGCTTAGCAATAG+AGG - chr4.4:16845208-16845227 MS.gene023354:CDS 40.0%
! TAGTCTTAATAGCTGCGTGA+TGG - chr4.4:16845064-16845083 MS.gene023354:CDS 40.0%
! TGTTGATGGAGAAACAAGCA+GGG - chr4.4:16845577-16845596 MS.gene023354:intron 40.0%
!! GAAGGTGCAATCGATAGTTT+TGG - chr4.4:16846592-16846611 MS.gene023354:CDS 40.0%
!! GATGGTAACATGTTTGCTGT+AGG - chr4.4:16845770-16845789 MS.gene023354:intron 40.0%
!! GTGATGAAAGGTGTTCAACA+TGG - chr4.4:16845599-16845618 MS.gene023354:intron 40.0%
!! TCATTCCAAGAGTACTGTGT+TGG + chr4.4:16846784-16846803 None:intergenic 40.0%
AGCTAGAGTAGTTTGGTCTG+TGG - chr4.4:16845880-16845899 MS.gene023354:CDS 45.0%
AGCTTGAAACCATCCATGTC+AGG + chr4.4:16845296-16845315 None:intergenic 45.0%
CCATACCAACACAGTACTCT+TGG - chr4.4:16846776-16846795 MS.gene023354:intron 45.0%
CCTAGAACTAGAAGGCTAGT+AGG - chr4.4:16846294-16846313 MS.gene023354:intron 45.0%
CGATGTAAACATGCCTGACA+TGG - chr4.4:16845280-16845299 MS.gene023354:CDS 45.0%
CTCATCCTAGCTAGCGTTAT+TGG + chr4.4:16844924-16844943 None:intergenic 45.0%
CTGTTAGGCCTAGAACTAGA+AGG - chr4.4:16846286-16846305 MS.gene023354:intron 45.0%
GAAACAAGCAGGGTGATGAA+AGG - chr4.4:16845587-16845606 MS.gene023354:intron 45.0%
GAAGACACCGATGAATTCAC+AGG + chr4.4:16846967-16846986 None:intergenic 45.0%
GCAACCTACTCAAGTATAGC+CGG + chr4.4:16846462-16846481 None:intergenic 45.0%
GGATCAGATCACACTGATGA+TGG - chr4.4:16845752-16845771 MS.gene023354:intron 45.0%
GGTAGGTCTTAACCAATCTC+TGG - chr4.4:16846720-16846739 MS.gene023354:intron 45.0%
GTAAACATGCCTGACATGGA+TGG - chr4.4:16845284-16845303 MS.gene023354:CDS 45.0%
GTGTTCTGTTCTTCTTCGAG+TGG + chr4.4:16846820-16846839 None:intergenic 45.0%
TGGTGGAGTCAAATGCTGTA+TGG + chr4.4:16846759-16846778 None:intergenic 45.0%
! AAAGCATCAAGGTGTGGAGA+AGG + chr4.4:16844573-16844592 None:intergenic 45.0%
! ATGAGGCTTTTGGACTGACT+AGG + chr4.4:16847003-16847022 None:intergenic 45.0%
! CTGTTGATGGAGAAACAAGC+AGG - chr4.4:16845576-16845595 MS.gene023354:intron 45.0%
! GCTTTTGGACTGACTAGGAA+AGG + chr4.4:16846998-16847017 None:intergenic 45.0%
!! AGAGTACTGTGTTGGTATGG+TGG + chr4.4:16846776-16846795 None:intergenic 45.0%
!! CCAAGAGTACTGTGTTGGTA+TGG + chr4.4:16846779-16846798 None:intergenic 45.0%
!! CCTACTAGCCTTCTAGTTCT+AGG + chr4.4:16846297-16846316 None:intergenic 45.0%
ACTGGTCCGGAAAAAAGAGC+TGG + chr4.4:16845415-16845434 None:intergenic 50.0%
CTGGTCCGGAAAAAAGAGCT+GGG + chr4.4:16845414-16845433 None:intergenic 50.0%
TAAGTCCTCGTCCAAAGGTG+TGG + chr4.4:16847157-16847176 None:intergenic 50.0%
TGAGTCAGCTTCCACACCTT+TGG - chr4.4:16847143-16847162 MS.gene023354:intron 50.0%
! CACCTTGATGCTTTTCCAGC+TGG - chr4.4:16844579-16844598 MS.gene023354:CDS 50.0%
!! CTAGTAGGTACTGACTGGTC+CGG + chr4.4:16845428-16845447 None:intergenic 50.0%
!! GACCAGCTGGAAAAGCATCA+AGG + chr4.4:16844584-16844603 None:intergenic 50.0%
!! GCTGGAAAAGCATCAAGGTG+TGG + chr4.4:16844579-16844598 None:intergenic 50.0%
!!! CCTCTCCCAGCTCTTTTTTC+CGG - chr4.4:16845406-16845425 MS.gene023354:intron 50.0%
ACGAGAACTCGTAGACCAGC+TGG + chr4.4:16844597-16844616 None:intergenic 55.0%
AGCTTCCACACCTTTGGACG+AGG - chr4.4:16847149-16847168 MS.gene023354:CDS 55.0%
CCGGAAAAAAGAGCTGGGAG+AGG + chr4.4:16845409-16845428 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 16844519 16847308 16844519 ID=MS.gene023354
chr4.4 mRNA 16844519 16847308 16844519 ID=MS.gene023354.t1;Parent=MS.gene023354
chr4.4 exon 16847146 16847308 16847146 ID=MS.gene023354.t1.exon1;Parent=MS.gene023354.t1
chr4.4 CDS 16847146 16847308 16847146 ID=cds.MS.gene023354.t1;Parent=MS.gene023354.t1
chr4.4 exon 16846471 16846623 16846471 ID=MS.gene023354.t1.exon2;Parent=MS.gene023354.t1
chr4.4 CDS 16846471 16846623 16846471 ID=cds.MS.gene023354.t1;Parent=MS.gene023354.t1
chr4.4 exon 16845877 16846257 16845877 ID=MS.gene023354.t1.exon3;Parent=MS.gene023354.t1
chr4.4 CDS 16845877 16846257 16845877 ID=cds.MS.gene023354.t1;Parent=MS.gene023354.t1
chr4.4 exon 16845618 16845694 16845618 ID=MS.gene023354.t1.exon4;Parent=MS.gene023354.t1
chr4.4 CDS 16845618 16845694 16845618 ID=cds.MS.gene023354.t1;Parent=MS.gene023354.t1
chr4.4 exon 16844519 16845373 16844519 ID=MS.gene023354.t1.exon5;Parent=MS.gene023354.t1
chr4.4 CDS 16844519 16845373 16844519 ID=cds.MS.gene023354.t1;Parent=MS.gene023354.t1
Gene Sequence

>MS.gene023354

ATGGTACACAACAGGTCTCTTGTCTATGACAACATGGATAATTCTTGCTTTCCTTCTCCACACCTTGATGCTTTTCCAGCTGGTCTACGAGTTCTCGTTGTTGATGATGATCTAACTTGGCTCAGAATCCTTGAAAAAATGCTAAAAAAATGCTTTTACCGAGGTTTCTTTCTTTCATTCATATTCACTCTGCTTCTTCTTCCATGTGCTAAAAGTGGTATATATGAATCTGACTGAATTAATCTCAACAAATGCCAATAAGTTCAGTACAATTCAACTTTAAGAAAAGCAAGCATTTCACCAATCCACATTTTTGTTGGCTTCTAATTTCCAATTCCTTTTTTTTTTTTCAAAGAAAAAATGAATCTTGTTACTTGTTCATTTAAGATAATTTTGTACTAACCAATAACGCTAGCTAGGATGAGATGATAATACTGGTTTCTTTTAACTTAATTAACAAAGGTGTTTAAATTCACACTCATAACAACGTTAAAGCTATTGAAGAAGAGTTTTACGACTCATGTGTCTTATTTGGTTCGAGATATTAGTCTTAATAGCTGCGTGATGGGATACCTAATTTATACTAAAAAAAAACTTCAAAGCTGTTTTTTCTTAAATTTGCAAATTTTGGAAGCAAACACATCTTATATTTATCTTCTAACAGTTATCTATCATGTGCTCTCAGTGACCACATGTTGCTTAGCAATAGAGGCTCTTAAGAAGCTCCGCGAAAGTAAAGATGCATATGACATAGTGATCAGCGATGTAAACATGCCTGACATGGATGGTTTCAAGCTTCTTGAACATGTTGGACTTGAGATGGATCTTCCTGTCATTAGTAAGTTCTTTTATTAATAACATTTTCGCTTCAAATTATGTGTAACATTCCTCTCCCAGCTCTTTTTTCCGGACCAGTCAGTACCTACTAGTAATATTCTTGGTCCGAAAAAAAAAGCTAGAAGACAAGTGTTACAATATATGTACATAATCTTTTGATTTTATTGACTAAAATTCCATTATTTTACAAACAACACTTGATAATGTTATGCAGTGATGTCTGTTGATGGAGAAACAAGCAGGGTGATGAAAGGTGTTCAACATGGAGCATGTGATTATTTGCTTAAGCCTATAAGGATGAAAGAATTGCGAAACATATGGCAACATGTCTTAAGAAAAAGAATACATGAGGCAAGAGAATTTGAAAGTCTCGAGGGAATTCACTTAATGAGAAATGGATCAGATCACACTGATGATGGTAACATGTTTGCTGTAGGAAAAGAAATGAACTTAATGAAGAAAAGGAAAAATTTCGATAGTAAACATGATGACCAAGAACTCGTAGATCGAACTTTCATAAAGAAAGCTAGAGTAGTTTGGTCTGTGGATCTTCATCAGAAGTTTGTTAAAGCTGTGAATCAACTTGGATTCGATAGTAAGTTTCTTATCAACATTCTCTATGCAGTCACATTCAAATAAATCTACTCATTGCCAAGCACACTAAATAAATCTACTTTTATCATATTATTGTTCTTGTTAATTTTATGTATGAAGTTATGAACCATCATATCTTTTGTGTAAGAGAATGTAATGATAAAAATAATTTCTTTTTGGCAGAAGTTGGTCCCAAGAAAATTCTAGATTTGATGAATGTTCCGTGGCTGACTAGAGAAAATGTTGCTAGTCACTTGCAGGTATATGATTTGTATTTGTGAACCTCTACTAAAGTTAAAAGTTTATATTATGAGTATTGCATCTGATATGTTGTTTCTGTTAGGCCTAGAACTAGAAGGCTAGTAGGGGTTCAATTTAAATTAAGCATCCAATACACATCTCAGAAATATTGACATAAATGCAGACTATCTTATAAAATTCAAATTGAATAAATTTTGAGTTTGAAAACTTACAGGTTCCTAATAATAAAATTTTGCTCTTCAGAAATACCGGCTATACTTGAGTAGGTTGCAAAAGAAAAATGATCAGAAATTGTCCTCAGGTAGAATAAAGCATTCAGATTTACCTTCAAAAGATCCAGGATGTTTAAGCTTTCTCAACTCTTTCAGCAAGCAACAAAACGAAGGTGCAATCGATAGTTTTGGATATTCAGATGGGACATTAAAAATTCAAATGGATACTACAGGTCATGGAAAAAGAAGAGCTTCAACTAGTGAAATTTCTGATCCAAAGATAAGAAAGAGTTCAGAGGTAGGTCTTAACCAATCTCTGGAATCAGAAGTAAACCATACAGCATTTGACTCCACCATACCAACACAGTACTCTTGGAATGAAGTTTCTAAAACGCCACTCGAAGAAGAACAGAACACACTTGTACAATTAAAGGATAGCTTCAGCCATTTGCCATTAAATGTTACACAGCAACACTTCCAAATTGACCAATCACTTTCAATTAATTCCAATCCCTCCGATTACAAGAATGATTACATTAGTCCTGTGAATTCATCGGTGTCTTCAGTTGATACCTTTCCTAGTCAGTCCAAAAGCCTCATCTCAAATGATCAATCTCCTGATATCATTTTAACTTCAAATTTGAGTTCAAAAACACCAAGATTTAATCTTGGTTGCATTTATGATATGGAAATCTACCAAAGAAACTTACTTTTGGGTATTGAGTCAGCTTCCACACCTTTGGACGAGGACTTAAACTTCCTTTTGTTACAAGGTGAATATGATATGAACTTTGACATGCAGAATATAGAGATGTCAGAGTATTATGATCCAAGGTTTATTGCTGAAGTTCCTACCAATTTGTATGCTTCAGCAGATTATTCTTAG
Protein sequence

>MS.gene023354.t1

MVHNRSLVYDNMDNSCFPSPHLDAFPAGLRVLVVDDDLTWLRILEKMLKKCFYRVTTCCLAIEALKKLRESKDAYDIVISDVNMPDMDGFKLLEHVGLEMDLPVIMMSVDGETSRVMKGVQHGACDYLLKPIRMKELRNIWQHVLRKRIHEAREFESLEGIHLMRNGSDHTDDGNMFAVGKEMNLMKKRKNFDSKHDDQELVDRTFIKKARVVWSVDLHQKFVKAVNQLGFDKVGPKKILDLMNVPWLTRENVASHLQKYRLYLSRLQKKNDQKLSSGRIKHSDLPSKDPGCLSFLNSFSKQQNEGAIDSFGYSDGTLKIQMDTTGHGKRRASTSEISDPKIRKSSEVGLNQSLESEVNHTAFDSTIPTQYSWNEVSKTPLEEEQNTLVQLKDSFSHLPLNVTQQHFQIDQSLSINSNPSDYKNDYISPVNSSVSSVDTFPSQSKSLISNDQSPDIILTSNLSSKTPRFNLGCIYDMEIYQRNLLLGIESASTPLDEDLNFLLLQGEYDMNFDMQNIEMSEYYDPRFIAEVPTNLYASADYS