AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023628

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023628.t1 MTR_8g087770 96.889 675 20 1 1 675 1 674 0.0 1328
MS.gene023628.t1 MTR_8g087750 82.143 672 110 5 6 672 11 677 0.0 1109
MS.gene023628.t1 MTR_8g087763 76.991 226 51 1 374 599 4 228 7.01e-124 372
MS.gene023628.t1 MTR_4g093750 37.846 325 180 9 67 376 35 352 6.92e-42 162
MS.gene023628.t1 MTR_4g093750 39.726 292 154 9 67 343 35 319 2.55e-41 161
MS.gene023628.t1 MTR_2g009690 36.624 314 187 6 63 366 40 351 1.94e-40 159
MS.gene023628.t1 MTR_1g032550 35.689 283 162 7 69 337 35 311 1.08e-33 137
MS.gene023628.t1 MTR_8g105580 35.816 282 162 6 69 337 35 310 1.96e-33 135
MS.gene023628.t1 MTR_8g105580 35.816 282 162 6 69 337 35 310 2.00e-33 136
MS.gene023628.t1 MTR_5g009150 42.473 186 105 2 69 252 35 220 2.71e-32 132
MS.gene023628.t1 MTR_7g451010 35.616 292 164 8 69 346 40 321 3.95e-32 132
MS.gene023628.t1 MTR_8g099550 42.632 190 99 3 69 252 34 219 1.46e-31 130
MS.gene023628.t1 MTR_8g009510 34.810 316 182 9 69 369 40 346 2.01e-31 130
MS.gene023628.t1 MTR_8g027675 42.211 199 104 5 69 264 34 224 2.31e-31 130
MS.gene023628.t1 MTR_4g085860 75.641 78 18 1 206 282 2 79 7.19e-31 115
MS.gene023628.t1 MTR_4g098920 34.483 319 179 10 69 369 34 340 7.57e-31 128
MS.gene023628.t1 MTR_6g045107 74.359 78 19 1 206 282 2 79 6.21e-29 110
MS.gene023628.t1 MTR_8g463270 30.736 231 141 8 54 269 27 253 1.16e-16 84.7
MS.gene023628.t1 MTR_8g009510 30.488 246 151 6 136 369 2 239 4.87e-16 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023628.t1 AT1G60500 56.101 672 283 4 3 668 2 667 0.0 768
MS.gene023628.t1 AT1G60530 72.069 290 73 1 18 307 19 300 3.94e-143 419
MS.gene023628.t1 AT4G33650 30.485 433 277 10 35 452 29 452 8.24e-40 157
MS.gene023628.t1 AT4G33650 30.485 433 277 10 35 452 29 452 9.50e-40 157
MS.gene023628.t1 AT2G14120 32.647 340 205 7 25 346 1 334 4.29e-39 154
MS.gene023628.t1 AT2G14120 32.022 356 217 8 25 362 1 349 5.60e-39 154
MS.gene023628.t1 AT2G14120 32.647 340 205 7 25 346 1 334 6.12e-39 154
MS.gene023628.t1 AT2G14120 32.022 356 217 8 25 362 1 349 6.29e-39 154
MS.gene023628.t1 AT1G14830 35.252 278 170 5 69 337 35 311 2.75e-35 142
MS.gene023628.t1 AT3G60190 33.226 310 195 6 69 369 40 346 1.58e-32 134
MS.gene023628.t1 AT5G42080 33.871 310 193 6 69 369 34 340 2.43e-32 130
MS.gene023628.t1 AT5G42080 33.871 310 193 6 69 369 34 340 6.22e-32 132
MS.gene023628.t1 AT5G42080 33.871 310 193 6 69 369 34 340 6.22e-32 132
MS.gene023628.t1 AT5G42080 33.871 310 193 6 69 369 34 340 6.48e-32 131
MS.gene023628.t1 AT2G44590 32.986 288 175 7 69 345 35 315 2.93e-31 129
MS.gene023628.t1 AT3G61760 41.667 204 109 5 69 268 34 231 8.11e-31 126
MS.gene023628.t1 AT3G61760 41.667 204 109 5 69 268 34 231 1.00e-30 128
MS.gene023628.t1 AT3G61760 41.667 204 109 5 69 268 34 231 1.29e-30 127
MS.gene023628.t1 AT2G44590 29.861 288 167 8 69 345 35 298 8.64e-22 100
MS.gene023628.t1 AT2G44590 29.861 288 167 8 69 345 35 298 9.90e-22 100
MS.gene023628.t1 AT1G53140 28.151 238 155 8 44 269 36 269 1.96e-15 80.9

Find 126 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGTCTCCATCTATTTC+TGG 0.166668 4.4:+13146512 MS.gene023628:CDS
CATTGTTGGTATTCCTGTTT+TGG 0.198486 4.4:+13145515 MS.gene023628:CDS
GCAGTTCTTGGCATGAAATT+TGG 0.208693 4.4:-13145912 None:intergenic
TTAGTACTTGTGAGTCTATT+AGG 0.242558 4.4:+13145265 MS.gene023628:CDS
ATCAAGTTTGTTTGCCTTTG+TGG 0.244719 4.4:-13144629 None:intergenic
CTTGAGGAAGCTAAGTTAAT+TGG 0.255531 4.4:+13145885 MS.gene023628:CDS
GCCTGCCAATTTGACTTCAT+TGG 0.264901 4.4:+13145647 MS.gene023628:CDS
TGACAATGTGTGGAGCTATT+TGG 0.298324 4.4:+13145995 MS.gene023628:CDS
TCTGAGAAAGATTCTTCTAA+TGG 0.298751 4.4:+13145719 MS.gene023628:CDS
AATGTTAGAAAAGCAGTTCT+TGG 0.299019 4.4:-13145924 None:intergenic
ATCTGATCATAAATGTTATC+AGG 0.304162 4.4:-13145168 None:intergenic
TAACATTTATGATCAGATTA+AGG 0.317964 4.4:+13145173 MS.gene023628:CDS
CTTCCAACCATTGTTGTTGT+TGG 0.322456 4.4:+13144811 MS.gene023628:CDS
TGATGATGTTAACATTGGTT+TGG 0.323376 4.4:+13145383 MS.gene023628:CDS
TTCATTTCTTGCTTCTTCAT+AGG 0.344245 4.4:-13145442 None:intergenic
AGAGACTGTGGCCAACATTA+TGG 0.346194 4.4:+13146583 MS.gene023628:CDS
ATCAGCCAATGAAGTCAAAT+TGG 0.365085 4.4:-13145652 None:intergenic
CAAGGGTACCTTTGGTTATG+AGG 0.366182 4.4:+13144911 MS.gene023628:CDS
CAATGCATTTGTTTATCATC+AGG 0.370755 4.4:-13145759 None:intergenic
TGCAAACTATTACCAGTTGC+AGG 0.374084 4.4:+13146052 MS.gene023628:CDS
CTTACAATGGTTGATCTTCC+AGG 0.382214 4.4:+13145117 MS.gene023628:CDS
ACTTTCTCCAACAAACCTTC+AGG 0.384947 4.4:-13145354 None:intergenic
CTGAGAAAGATTCTTCTAAT+GGG 0.390787 4.4:+13145720 MS.gene023628:CDS
GATGATGTTAACATTGGTTT+GGG 0.391771 4.4:+13145384 MS.gene023628:CDS
GCTCTGGCTGTGGAAGTAAA+TGG 0.394731 4.4:-13144942 None:intergenic
TTCCATTAGGAAATCCTTTG+TGG 0.394982 4.4:-13145850 None:intergenic
TTGATCTTCTTAATTATCTC+AGG 0.396848 4.4:-13145585 None:intergenic
TCACAGATAGTTGATAAAAC+TGG 0.398319 4.4:+13145291 MS.gene023628:CDS
AGGCAAGATTAATTGCCTAT+TGG 0.399341 4.4:+13146360 MS.gene023628:CDS
AAGATTGACAAATCCATTGT+TGG 0.402415 4.4:+13145501 MS.gene023628:CDS
TCCGATCAATGTGTTGAATC+AGG 0.406853 4.4:+13146325 MS.gene023628:CDS
TTGAGGATAATGCTCTCTTC+AGG 0.409426 4.4:-13145219 None:intergenic
GAATCAGGCCTTTGACTTGA+AGG 0.413968 4.4:+13146340 MS.gene023628:CDS
ATGGACAGGATTGGAGTCTA+TGG 0.415221 4.4:+13146602 MS.gene023628:CDS
TTGATGCTCTTGAAAATCTA+AGG 0.416842 4.4:+13144758 MS.gene023628:CDS
TCCACAGCCAGAGCTTGTAT+TGG 0.421767 4.4:+13144951 MS.gene023628:CDS
TTGCAATCCGGAGTACTTGC+AGG 0.423704 4.4:+13146178 MS.gene023628:CDS
AATTTGTACAAGGGTACCTT+TGG 0.424069 4.4:+13144903 MS.gene023628:CDS
TCAAGTACTGTGAAGCTTGA+AGG 0.424080 4.4:+13146266 MS.gene023628:CDS
ACAGCTACTGAAGTGCTTGC+TGG 0.424935 4.4:+13145030 MS.gene023628:CDS
TGTCTCTTACCAGAAATAGA+TGG 0.425419 4.4:-13146521 None:intergenic
GAGCTTGTATTGGAGTATAA+TGG 0.426836 4.4:+13144961 MS.gene023628:CDS
GGACTACGATCTCTTCCATT+AGG 0.434291 4.4:-13145863 None:intergenic
TGTGATGCAAGCTGTTGATA+TGG 0.436114 4.4:+13146136 MS.gene023628:CDS
TAAATCACCTGAAGGTTTGT+TGG 0.440077 4.4:+13145347 MS.gene023628:CDS
TTTGGAAAGGGTTGTTATCT+CGG 0.442091 4.4:+13146013 MS.gene023628:CDS
CCTCAGCACAATCTTCCAAT+AGG 0.443004 4.4:-13146375 None:intergenic
TTCCACAAAGGATTTCCTAA+TGG 0.449900 4.4:+13145848 MS.gene023628:CDS
ACATTGTCAACAAAATTAGT+TGG 0.454264 4.4:-13145981 None:intergenic
GCATTGTACTGCTCGGTTAG+TGG 0.455271 4.4:+13145776 MS.gene023628:CDS
TCATTATAAGAAGATACAAT+TGG 0.458646 4.4:-13144718 None:intergenic
ATTGGAGTATAATGGCAAAC+AGG 0.459418 4.4:+13144969 MS.gene023628:CDS
TACGAGTAGCAACCTGCAAC+TGG 0.461514 4.4:-13146064 None:intergenic
ACAATTGGTGCAACAGATAG+AGG 0.467988 4.4:-13144703 None:intergenic
ACAAGCTTCTGAGCCAAAAC+AGG 0.473524 4.4:-13145528 None:intergenic
TGATACCAGCTAATGATTCA+AGG 0.474056 4.4:-13144861 None:intergenic
GTTGTTGGAGATCAATCATC+TGG 0.485196 4.4:+13144826 MS.gene023628:CDS
TGGAGTGTTTGAAATCCCTT+TGG 0.490204 4.4:-13145058 None:intergenic
TTATACTCCAATACAAGCTC+TGG 0.492061 4.4:-13144958 None:intergenic
GCCAATGAAGTCAAATTGGC+AGG 0.500514 4.4:-13145648 None:intergenic
GCGTCTCAACATTGCTAAAG+AGG 0.501157 4.4:+13144780 MS.gene023628:CDS
AGCTTGCATCACATGTTGAA+TGG 0.501675 4.4:-13146126 None:intergenic
CACGGATTACACTTGCAATC+CGG 0.511102 4.4:+13146166 MS.gene023628:CDS
CTGGAAGATCAACCATTGTA+AGG 0.513573 4.4:-13145116 None:intergenic
ATAACTCGTGTTCCTGTACA+TGG 0.514492 4.4:+13145141 MS.gene023628:CDS
TTTGTGTGAGAAATCGCATT+GGG 0.515555 4.4:+13145412 MS.gene023628:CDS
GTAAGAGACAAAGTTTAAGC+AGG 0.516534 4.4:+13146534 MS.gene023628:CDS
AATGCATTTGTTTATCATCA+GGG 0.517273 4.4:-13145758 None:intergenic
TCCAATACAAGCTCTGGCTG+TGG 0.519083 4.4:-13144952 None:intergenic
TCCAATCCTGTCCATAATGT+TGG 0.522411 4.4:-13146594 None:intergenic
AGTGTCCTTGAATCATTAGC+TGG 0.523168 4.4:+13144856 MS.gene023628:CDS
GGTGCAACAGATAGAGGTTG+AGG 0.525331 4.4:-13144697 None:intergenic
GTGCTTGCTGGAACTGCCAA+AGG 0.525520 4.4:+13145042 MS.gene023628:CDS
AGATCAACCATTGTAAGGTC+AGG 0.531215 4.4:-13145111 None:intergenic
TAATCTTGCCTTCAAGTCAA+AGG 0.534214 4.4:-13146348 None:intergenic
GCCAACATTATGGACAGGAT+TGG 0.534644 4.4:+13146593 MS.gene023628:CDS
GCCTGATTCAACACATTGAT+CGG 0.536471 4.4:-13146326 None:intergenic
AAGGAGATTTGTAATGAACT+TGG 0.538785 4.4:+13146467 MS.gene023628:CDS
ACTTTGATTGTGAAGAAGAA+TGG 0.539337 4.4:+13145084 MS.gene023628:CDS
ATCAGCTTGCCACGTGGACA+AGG 0.540401 4.4:+13144880 MS.gene023628:CDS
TCAGATTAAGGATATTATCA+TGG 0.543580 4.4:+13145185 MS.gene023628:CDS
TTATATTCCTGCAAGTACTC+CGG 0.543933 4.4:-13146185 None:intergenic
ATGTGTGGAGCTATTTGGAA+AGG 0.549884 4.4:+13146000 MS.gene023628:CDS
AACAAATGCATTGTACTGCT+CGG 0.550416 4.4:+13145769 MS.gene023628:CDS
TCTCCAACAACAACAATGGT+TGG 0.551269 4.4:-13144814 None:intergenic
GAGATTTGTAATGAACTTGG+TGG 0.560761 4.4:+13146470 MS.gene023628:CDS
TTCTTCACAATCAAAGTCAG+TGG 0.564699 4.4:-13145078 None:intergenic
TAGTTGATAAAACTGGTTTG+AGG 0.569831 4.4:+13145298 MS.gene023628:CDS
GTACTGCTCGGTTAGTGGAG+AGG 0.571484 4.4:+13145781 MS.gene023628:CDS
CTGTGGCCAACATTATGGAC+AGG 0.573065 4.4:+13146588 MS.gene023628:CDS
GACATTAGCTGTTGTAACAA+AGG 0.576294 4.4:+13145320 MS.gene023628:CDS
ATTACTTCAGAGAAAAGTGA+AGG 0.577066 4.4:+13145944 MS.gene023628:CDS
TTATCAGGTTGACCATGTAC+AGG 0.577742 4.4:-13145153 None:intergenic
TGCTTGCTGGAACTGCCAAA+GGG 0.584310 4.4:+13145043 MS.gene023628:CDS
CGTCTCAACATTGCTAAAGA+GGG 0.585949 4.4:+13144781 MS.gene023628:CDS
GTGGACAAGGAATTTGTACA+AGG 0.592993 4.4:+13144893 MS.gene023628:CDS
CAGTGTTGAGAGGCTGCTTG+AGG 0.594487 4.4:+13146493 MS.gene023628:CDS
GGAAGAGATCGTAGTCCTTG+AGG 0.596070 4.4:+13145869 MS.gene023628:CDS
ATTGGAAGATTGTGCTGAGG+AGG 0.597029 4.4:+13146378 MS.gene023628:CDS
ACTGTGAAGCTTGAAGGAGT+TGG 0.602736 4.4:+13146272 MS.gene023628:CDS
TGTGAGAAATCGCATTGGGG+AGG 0.608396 4.4:+13145416 MS.gene023628:CDS
GGTTGCTACTCGTAGAGCTG+TGG 0.612719 4.4:+13146073 MS.gene023628:CDS
TGTGTGGAGCTATTTGGAAA+GGG 0.616400 4.4:+13146001 MS.gene023628:CDS
CAATTAACTTAGCTTCCTCA+AGG 0.618556 4.4:-13145884 None:intergenic
ACAGGAACACGAGTTATACC+TGG 0.620303 4.4:-13145135 None:intergenic
CAAAACAGGAATACCAACAA+TGG 0.623000 4.4:-13145514 None:intergenic
GATTAACAATGATCTGCAGA+AGG 0.625104 4.4:+13146448 MS.gene023628:CDS
ACTGCTGATGATGTTAACAT+TGG 0.625783 4.4:+13145378 MS.gene023628:CDS
AAGGCTGATAAATCACCTGA+AGG 0.627415 4.4:+13145339 MS.gene023628:CDS
TTGATCTCCAACAACAACAA+TGG 0.648908 4.4:-13144818 None:intergenic
TAGAGAAAGCAAAGAGACTG+TGG 0.654791 4.4:+13146571 MS.gene023628:CDS
GTACAAATTCCTTGTCCACG+TGG 0.660130 4.4:-13144889 None:intergenic
TTACTTCAGAGAAAAGTGAA+GGG 0.662697 4.4:+13145945 MS.gene023628:CDS
TGTTGATATGGAGAAGCACA+CGG 0.665028 4.4:+13146148 MS.gene023628:CDS
AACTTGGTGGCAGTGTTGAG+AGG 0.665158 4.4:+13146483 MS.gene023628:CDS
CAGTAGCAGAAAGAACATTG+AGG 0.676780 4.4:-13145236 None:intergenic
GTTTGTGTGAGAAATCGCAT+TGG 0.677044 4.4:+13145411 MS.gene023628:CDS
TGGTGTTCCTGACCTTACAA+TGG 0.692132 4.4:+13145104 MS.gene023628:CDS
TGCTGAAAGTGATTCCACAA+AGG 0.697479 4.4:+13145836 MS.gene023628:CDS
GCTGGTATCAGCTTGCCACG+TGG 0.699523 4.4:+13144874 MS.gene023628:CDS
GTTGATCATCAACATCAACA+TGG 0.706155 4.4:-13144675 None:intergenic
TGGAAACAAGAGTTCCACAA+AGG 0.707464 4.4:+13144615 MS.gene023628:CDS
CCTATTGGAAGATTGTGCTG+AGG 0.716472 4.4:+13146375 MS.gene023628:CDS
TGGACAAGGAATTTGTACAA+GGG 0.728664 4.4:+13144894 MS.gene023628:CDS
TTGTGTGAGAAATCGCATTG+GGG 0.740526 4.4:+13145413 MS.gene023628:CDS
ACTGGTAATAGTTTGCAGAG+TGG 0.741343 4.4:-13146046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACATTTATGATCAGATTA+AGG + chr4.4:13145173-13145192 MS.gene023628:CDS 20.0%
!! TCATTATAAGAAGATACAAT+TGG - chr4.4:13144721-13144740 None:intergenic 20.0%
!!! AGCATTTTTGAAAAAAGTTT+TGG + chr4.4:13146229-13146248 MS.gene023628:CDS 20.0%
! AATGCATTTGTTTATCATCA+GGG - chr4.4:13145761-13145780 None:intergenic 25.0%
! ACATTGTCAACAAAATTAGT+TGG - chr4.4:13145984-13146003 None:intergenic 25.0%
! ATCTGATCATAAATGTTATC+AGG - chr4.4:13145171-13145190 None:intergenic 25.0%
! TCAGATTAAGGATATTATCA+TGG + chr4.4:13145185-13145204 MS.gene023628:CDS 25.0%
! TTGATCTTCTTAATTATCTC+AGG - chr4.4:13145588-13145607 None:intergenic 25.0%
AAGATTGACAAATCCATTGT+TGG + chr4.4:13145501-13145520 MS.gene023628:CDS 30.0%
AAGGAGATTTGTAATGAACT+TGG + chr4.4:13146467-13146486 MS.gene023628:CDS 30.0%
AATGTTAGAAAAGCAGTTCT+TGG - chr4.4:13145927-13145946 None:intergenic 30.0%
ACTTTGATTGTGAAGAAGAA+TGG + chr4.4:13145084-13145103 MS.gene023628:CDS 30.0%
ATTACTTCAGAGAAAAGTGA+AGG + chr4.4:13145944-13145963 MS.gene023628:CDS 30.0%
CAATGCATTTGTTTATCATC+AGG - chr4.4:13145762-13145781 None:intergenic 30.0%
CTGAGAAAGATTCTTCTAAT+GGG + chr4.4:13145720-13145739 MS.gene023628:CDS 30.0%
TCTGAGAAAGATTCTTCTAA+TGG + chr4.4:13145719-13145738 MS.gene023628:CDS 30.0%
TTACTTCAGAGAAAAGTGAA+GGG + chr4.4:13145945-13145964 MS.gene023628:CDS 30.0%
TTAGTACTTGTGAGTCTATT+AGG + chr4.4:13145265-13145284 MS.gene023628:CDS 30.0%
TTCATTTCTTGCTTCTTCAT+AGG - chr4.4:13145445-13145464 None:intergenic 30.0%
TTGATGCTCTTGAAAATCTA+AGG + chr4.4:13144758-13144777 MS.gene023628:CDS 30.0%
! CTAATTTTGTTGACAATGTG+TGG + chr4.4:13145985-13146004 MS.gene023628:CDS 30.0%
! TGATGATGTTAACATTGGTT+TGG + chr4.4:13145383-13145402 MS.gene023628:CDS 30.0%
!! GATGATGTTAACATTGGTTT+GGG + chr4.4:13145384-13145403 MS.gene023628:CDS 30.0%
!! TAGTTGATAAAACTGGTTTG+AGG + chr4.4:13145298-13145317 MS.gene023628:CDS 30.0%
!! TCACAGATAGTTGATAAAAC+TGG + chr4.4:13145291-13145310 MS.gene023628:CDS 30.0%
AACAAATGCATTGTACTGCT+CGG + chr4.4:13145769-13145788 MS.gene023628:CDS 35.0%
AGGCAAGATTAATTGCCTAT+TGG + chr4.4:13146360-13146379 MS.gene023628:CDS 35.0%
ATCAGCCAATGAAGTCAAAT+TGG - chr4.4:13145655-13145674 None:intergenic 35.0%
ATTGGAGTATAATGGCAAAC+AGG + chr4.4:13144969-13144988 MS.gene023628:CDS 35.0%
CAAAACAGGAATACCAACAA+TGG - chr4.4:13145517-13145536 None:intergenic 35.0%
CAATTAACTTAGCTTCCTCA+AGG - chr4.4:13145887-13145906 None:intergenic 35.0%
CTTGAGGAAGCTAAGTTAAT+TGG + chr4.4:13145885-13145904 MS.gene023628:CDS 35.0%
GACATTAGCTGTTGTAACAA+AGG + chr4.4:13145320-13145339 MS.gene023628:CDS 35.0%
GAGATTTGTAATGAACTTGG+TGG + chr4.4:13146470-13146489 MS.gene023628:CDS 35.0%
GAGCTTGTATTGGAGTATAA+TGG + chr4.4:13144961-13144980 MS.gene023628:CDS 35.0%
GATTAACAATGATCTGCAGA+AGG + chr4.4:13146448-13146467 MS.gene023628:CDS 35.0%
GTAAGAGACAAAGTTTAAGC+AGG + chr4.4:13146534-13146553 MS.gene023628:CDS 35.0%
GTTGATCATCAACATCAACA+TGG - chr4.4:13144678-13144697 None:intergenic 35.0%
TAAATCACCTGAAGGTTTGT+TGG + chr4.4:13145347-13145366 MS.gene023628:CDS 35.0%
TAATCTTGCCTTCAAGTCAA+AGG - chr4.4:13146351-13146370 None:intergenic 35.0%
TGATACCAGCTAATGATTCA+AGG - chr4.4:13144864-13144883 None:intergenic 35.0%
TGGACAAGGAATTTGTACAA+GGG + chr4.4:13144894-13144913 MS.gene023628:CDS 35.0%
TGTCTCTTACCAGAAATAGA+TGG - chr4.4:13146524-13146543 None:intergenic 35.0%
TTATACTCCAATACAAGCTC+TGG - chr4.4:13144961-13144980 None:intergenic 35.0%
TTATATTCCTGCAAGTACTC+CGG - chr4.4:13146188-13146207 None:intergenic 35.0%
TTCCACAAAGGATTTCCTAA+TGG + chr4.4:13145848-13145867 MS.gene023628:CDS 35.0%
TTCCATTAGGAAATCCTTTG+TGG - chr4.4:13145853-13145872 None:intergenic 35.0%
TTCTTCACAATCAAAGTCAG+TGG - chr4.4:13145081-13145100 None:intergenic 35.0%
TTGATCTCCAACAACAACAA+TGG - chr4.4:13144821-13144840 None:intergenic 35.0%
TTTGGAAAGGGTTGTTATCT+CGG + chr4.4:13146013-13146032 MS.gene023628:CDS 35.0%
TTTGTGTGAGAAATCGCATT+GGG + chr4.4:13145412-13145431 MS.gene023628:CDS 35.0%
! ACTGCTGATGATGTTAACAT+TGG + chr4.4:13145378-13145397 MS.gene023628:CDS 35.0%
! ATCAAGTTTGTTTGCCTTTG+TGG - chr4.4:13144632-13144651 None:intergenic 35.0%
! CATTGTTGGTATTCCTGTTT+TGG + chr4.4:13145515-13145534 MS.gene023628:CDS 35.0%
!! AATTTGTACAAGGGTACCTT+TGG + chr4.4:13144903-13144922 MS.gene023628:CDS 35.0%
AAGGCTGATAAATCACCTGA+AGG + chr4.4:13145339-13145358 MS.gene023628:CDS 40.0%
ACAATTGGTGCAACAGATAG+AGG - chr4.4:13144706-13144725 None:intergenic 40.0%
ACTTTCTCCAACAAACCTTC+AGG - chr4.4:13145357-13145376 None:intergenic 40.0%
AGATCAACCATTGTAAGGTC+AGG - chr4.4:13145114-13145133 None:intergenic 40.0%
AGCTTGCATCACATGTTGAA+TGG - chr4.4:13146129-13146148 None:intergenic 40.0%
AGTGTCCTTGAATCATTAGC+TGG + chr4.4:13144856-13144875 MS.gene023628:CDS 40.0%
ATAACTCGTGTTCCTGTACA+TGG + chr4.4:13145141-13145160 MS.gene023628:CDS 40.0%
ATGTGTGGAGCTATTTGGAA+AGG + chr4.4:13146000-13146019 MS.gene023628:CDS 40.0%
CAGTAGCAGAAAGAACATTG+AGG - chr4.4:13145239-13145258 None:intergenic 40.0%
CGTCTCAACATTGCTAAAGA+GGG + chr4.4:13144781-13144800 MS.gene023628:CDS 40.0%
CTGGAAGATCAACCATTGTA+AGG - chr4.4:13145119-13145138 None:intergenic 40.0%
CTTACAATGGTTGATCTTCC+AGG + chr4.4:13145117-13145136 MS.gene023628:CDS 40.0%
GCAGTTCTTGGCATGAAATT+TGG - chr4.4:13145915-13145934 None:intergenic 40.0%
GCCTGATTCAACACATTGAT+CGG - chr4.4:13146329-13146348 None:intergenic 40.0%
GTGGACAAGGAATTTGTACA+AGG + chr4.4:13144893-13144912 MS.gene023628:CDS 40.0%
GTTGTTGGAGATCAATCATC+TGG + chr4.4:13144826-13144845 MS.gene023628:CDS 40.0%
GTTTGTGTGAGAAATCGCAT+TGG + chr4.4:13145411-13145430 MS.gene023628:CDS 40.0%
TAGAGAAAGCAAAGAGACTG+TGG + chr4.4:13146571-13146590 MS.gene023628:CDS 40.0%
TCCAATCCTGTCCATAATGT+TGG - chr4.4:13146597-13146616 None:intergenic 40.0%
TCCGATCAATGTGTTGAATC+AGG + chr4.4:13146325-13146344 MS.gene023628:CDS 40.0%
TCTCCAACAACAACAATGGT+TGG - chr4.4:13144817-13144836 None:intergenic 40.0%
TGACAATGTGTGGAGCTATT+TGG + chr4.4:13145995-13146014 MS.gene023628:CDS 40.0%
TGCAAACTATTACCAGTTGC+AGG + chr4.4:13146052-13146071 MS.gene023628:CDS 40.0%
TGCTGAAAGTGATTCCACAA+AGG + chr4.4:13145836-13145855 MS.gene023628:CDS 40.0%
TGGAAACAAGAGTTCCACAA+AGG + chr4.4:13144615-13144634 MS.gene023628:CDS 40.0%
TGTGATGCAAGCTGTTGATA+TGG + chr4.4:13146136-13146155 MS.gene023628:CDS 40.0%
TGTGTGGAGCTATTTGGAAA+GGG + chr4.4:13146001-13146020 MS.gene023628:CDS 40.0%
TTGAGGATAATGCTCTCTTC+AGG - chr4.4:13145222-13145241 None:intergenic 40.0%
TTGTGTGAGAAATCGCATTG+GGG + chr4.4:13145413-13145432 MS.gene023628:CDS 40.0%
! ACTGGTAATAGTTTGCAGAG+TGG - chr4.4:13146049-13146068 None:intergenic 40.0%
! CTTCCAACCATTGTTGTTGT+TGG + chr4.4:13144811-13144830 MS.gene023628:CDS 40.0%
! TCAAGTACTGTGAAGCTTGA+AGG + chr4.4:13146266-13146285 MS.gene023628:CDS 40.0%
! TGGAGTGTTTGAAATCCCTT+TGG - chr4.4:13145061-13145080 None:intergenic 40.0%
! TGTTGATATGGAGAAGCACA+CGG + chr4.4:13146148-13146167 MS.gene023628:CDS 40.0%
! TTTTGCAGCCTCATAACCAA+AGG - chr4.4:13144922-13144941 None:intergenic 40.0%
!! TTATCAGGTTGACCATGTAC+AGG - chr4.4:13145156-13145175 None:intergenic 40.0%
!!! TTTTCAAGAGCATCAAGCAC+TGG - chr4.4:13144754-13144773 None:intergenic 40.0%
ACAAGCTTCTGAGCCAAAAC+AGG - chr4.4:13145531-13145550 None:intergenic 45.0%
ACAGGAACACGAGTTATACC+TGG - chr4.4:13145138-13145157 None:intergenic 45.0%
ACTGTGAAGCTTGAAGGAGT+TGG + chr4.4:13146272-13146291 MS.gene023628:CDS 45.0%
AGAGACTGTGGCCAACATTA+TGG + chr4.4:13146583-13146602 MS.gene023628:CDS 45.0%
ATGGACAGGATTGGAGTCTA+TGG + chr4.4:13146602-13146621 MS.gene023628:CDS 45.0%
ATTGGAAGATTGTGCTGAGG+AGG + chr4.4:13146378-13146397 MS.gene023628:CDS 45.0%
CCTATTGGAAGATTGTGCTG+AGG + chr4.4:13146375-13146394 MS.gene023628:CDS 45.0%
CCTCAGCACAATCTTCCAAT+AGG - chr4.4:13146378-13146397 None:intergenic 45.0%
GAGGAGTCTCCATCTATTTC+TGG + chr4.4:13146512-13146531 MS.gene023628:CDS 45.0%
GCCAACATTATGGACAGGAT+TGG + chr4.4:13146593-13146612 MS.gene023628:CDS 45.0%
GCCAATGAAGTCAAATTGGC+AGG - chr4.4:13145651-13145670 None:intergenic 45.0%
GCGTCTCAACATTGCTAAAG+AGG + chr4.4:13144780-13144799 MS.gene023628:CDS 45.0%
GGACTACGATCTCTTCCATT+AGG - chr4.4:13145866-13145885 None:intergenic 45.0%
GTACAAATTCCTTGTCCACG+TGG - chr4.4:13144892-13144911 None:intergenic 45.0%
TGGTGTTCCTGACCTTACAA+TGG + chr4.4:13145104-13145123 MS.gene023628:CDS 45.0%
! CACGGATTACACTTGCAATC+CGG + chr4.4:13146166-13146185 MS.gene023628:CDS 45.0%
! GCCTGCCAATTTGACTTCAT+TGG + chr4.4:13145647-13145666 MS.gene023628:CDS 45.0%
!! CAAGGGTACCTTTGGTTATG+AGG + chr4.4:13144911-13144930 MS.gene023628:CDS 45.0%
!! GAATCAGGCCTTTGACTTGA+AGG + chr4.4:13146340-13146359 MS.gene023628:CDS 45.0%
CTGTGGCCAACATTATGGAC+AGG + chr4.4:13146588-13146607 MS.gene023628:CDS 50.0%
GCATTGTACTGCTCGGTTAG+TGG + chr4.4:13145776-13145795 MS.gene023628:CDS 50.0%
GCTCTGGCTGTGGAAGTAAA+TGG - chr4.4:13144945-13144964 None:intergenic 50.0%
GGAAGAGATCGTAGTCCTTG+AGG + chr4.4:13145869-13145888 MS.gene023628:CDS 50.0%
GGTGCAACAGATAGAGGTTG+AGG - chr4.4:13144700-13144719 None:intergenic 50.0%
TACGAGTAGCAACCTGCAAC+TGG - chr4.4:13146067-13146086 None:intergenic 50.0%
TCCACAGCCAGAGCTTGTAT+TGG + chr4.4:13144951-13144970 MS.gene023628:CDS 50.0%
TGTGAGAAATCGCATTGGGG+AGG + chr4.4:13145416-13145435 MS.gene023628:CDS 50.0%
! AACTTGGTGGCAGTGTTGAG+AGG + chr4.4:13146483-13146502 MS.gene023628:CDS 50.0%
! ACAGCTACTGAAGTGCTTGC+TGG + chr4.4:13145030-13145049 MS.gene023628:CDS 50.0%
! TCCAATACAAGCTCTGGCTG+TGG - chr4.4:13144955-13144974 None:intergenic 50.0%
! TGCTTGCTGGAACTGCCAAA+GGG + chr4.4:13145043-13145062 MS.gene023628:CDS 50.0%
! TTGCAATCCGGAGTACTTGC+AGG + chr4.4:13146178-13146197 MS.gene023628:CDS 50.0%
ATCAGCTTGCCACGTGGACA+AGG + chr4.4:13144880-13144899 MS.gene023628:CDS 55.0%
GGTTGCTACTCGTAGAGCTG+TGG + chr4.4:13146073-13146092 MS.gene023628:CDS 55.0%
GTACTGCTCGGTTAGTGGAG+AGG + chr4.4:13145781-13145800 MS.gene023628:CDS 55.0%
! CAGTGTTGAGAGGCTGCTTG+AGG + chr4.4:13146493-13146512 MS.gene023628:CDS 55.0%
! GTGCTTGCTGGAACTGCCAA+AGG + chr4.4:13145042-13145061 MS.gene023628:CDS 55.0%
GCTGGTATCAGCTTGCCACG+TGG + chr4.4:13144874-13144893 MS.gene023628:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 13144607 13146634 13144607 ID=MS.gene023628
chr4.4 mRNA 13144607 13146634 13144607 ID=MS.gene023628.t1;Parent=MS.gene023628
chr4.4 exon 13144607 13146634 13144607 ID=MS.gene023628.t1.exon1;Parent=MS.gene023628.t1
chr4.4 CDS 13144607 13146634 13144607 ID=cds.MS.gene023628.t1;Parent=MS.gene023628.t1
Gene Sequence

>MS.gene023628

ATGACAATTGGAAACAAGAGTTCCACAAAGGCAAACAAACTTGATGAATCATCATCACTTGCATTAGTCCATGTTGATGTTGATGATCAACCTCAACCTCTATCTGTTGCACCAATTGTATCTTCTTATAATGAGAAGATACGTCCAGTGCTTGATGCTCTTGAAAATCTAAGGCGTCTCAACATTGCTAAAGAGGGAATTCAGCTTCCAACCATTGTTGTTGTTGGAGATCAATCATCTGGAAAGTCTAGTGTCCTTGAATCATTAGCTGGTATCAGCTTGCCACGTGGACAAGGAATTTGTACAAGGGTACCTTTGGTTATGAGGCTGCAAAACCATTTACTTCCACAGCCAGAGCTTGTATTGGAGTATAATGGCAAACAGGTTTTAACTGATGAAGCAAATGTCTCTGATGCTATTAACACAGCTACTGAAGTGCTTGCTGGAACTGCCAAAGGGATTTCAAACACTCCACTGACTTTGATTGTGAAGAAGAATGGTGTTCCTGACCTTACAATGGTTGATCTTCCAGGTATAACTCGTGTTCCTGTACATGGTCAACCTGATAACATTTATGATCAGATTAAGGATATTATCATGGAGTATATAACTCCTGAAGAGAGCATTATCCTCAATGTTCTTTCTGCTACTGTCGATTTTAGTACTTGTGAGTCTATTAGGATGTCACAGATAGTTGATAAAACTGGTTTGAGGACATTAGCTGTTGTAACAAAGGCTGATAAATCACCTGAAGGTTTGTTGGAGAAAGTAACTGCTGATGATGTTAACATTGGTTTGGGCTATGTTTGTGTGAGAAATCGCATTGGGGAGGAATCCTATGAAGAAGCAAGAAATGAAGAACAGAAGTTGTTTGAGTCTCACTCACTTCTTTCTAAGATTGACAAATCCATTGTTGGTATTCCTGTTTTGGCTCAGAAGCTTGTTCATGTGCAAGCTATGATTATTTCAAAAACTTTGCCTGAGATAATTAAGAAGATCAATGAGAAGCTTTATAATAGTGTACATGAGTTGAAGACTTTGCCTGCCAATTTGACTTCATTGGCTGATGCTATGTCTGCTTTTCTGCATATTGTTTCTCTGTCAAGAGATTCTCTGAGAAAGATTCTTCTAATGGGAGAATTTGAAGAATACCCTGATGATAAACAAATGCATTGTACTGCTCGGTTAGTGGAGAGGTTGAATTCATATGCAAGTGATCTTCAAAACTGTGCTGAAAGTGATTCCACAAAGGATTTCCTAATGGAAGAGATCGTAGTCCTTGAGGAAGCTAAGTTAATTGGTCTTCCAAATTTCATGCCAAGAACTGCTTTTCTAACATTACTTCAGAGAAAAGTGAAGGGCATTTCTCATATGCCAACTAATTTTGTTGACAATGTGTGGAGCTATTTGGAAAGGGTTGTTATCTCGGTCATGAATCGCCACTCTGCAAACTATTACCAGTTGCAGGTTGCTACTCGTAGAGCTGTGGAGCACGTTATTGCTAAGAAGAAGAGCAATTCCATTCAACATGTGATGCAAGCTGTTGATATGGAGAAGCACACGGATTACACTTGCAATCCGGAGTACTTGCAGGAATATAACGAGCTGATGTCTTATCAAGAAGCATTTTTGAAAAAAGTTTTGGATGATGATCGATGTTCAAGTACTGTGAAGCTTGAAGGAGTTGGTAACATTAAAGTTAATCATCTGATAGAGTATCCGATCAATGTGTTGAATCAGGCCTTTGACTTGAAGGCAAGATTAATTGCCTATTGGAAGATTGTGCTGAGGAGGCTCATTGATAGTATTGCTTTGCATTTGATGCTAAGCATCAATGAACTGATTAACAATGATCTGCAGAAGGAGATTTGTAATGAACTTGGTGGCAGTGTTGAGAGGCTGCTTGAGGAGTCTCCATCTATTTCTGGTAAGAGACAAAGTTTAAGCAGGAGTGTGAAAGTTCTTAGAGAAAGCAAAGAGACTGTGGCCAACATTATGGACAGGATTGGAGTCTATGGTAATAACTAG
Protein sequence

>MS.gene023628.t1

MTIGNKSSTKANKLDESSSLALVHVDVDDQPQPLSVAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLVMRLQNHLLPQPELVLEYNGKQVLTDEANVSDAINTATEVLAGTAKGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFSTCESIRMSQIVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGEESYEEARNEEQKLFESHSLLSKIDKSIVGIPVLAQKLVHVQAMIISKTLPEIIKKINEKLYNSVHELKTLPANLTSLADAMSAFLHIVSLSRDSLRKILLMGEFEEYPDDKQMHCTARLVERLNSYASDLQNCAESDSTKDFLMEEIVVLEEAKLIGLPNFMPRTAFLTLLQRKVKGISHMPTNFVDNVWSYLERVVISVMNRHSANYYQLQVATRRAVEHVIAKKKSNSIQHVMQAVDMEKHTDYTCNPEYLQEYNELMSYQEAFLKKVLDDDRCSSTVKLEGVGNIKVNHLIEYPINVLNQAFDLKARLIAYWKIVLRRLIDSIALHLMLSINELINNDLQKEICNELGGSVERLLEESPSISGKRQSLSRSVKVLRESKETVANIMDRIGVYGNN